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https://www.readbyqxmd.com/read/28339473/characterization-of-lignocellulolytic-activities-from-fungi-isolated-from-the-deep-sea-sponge-stelletta-normani
#1
Ramón Alberto Batista-García, Thomas Sutton, Stephen A Jackson, Omar Eduardo Tovar-Herrera, Edgar Balcázar-López, María Del Rayo Sánchez-Carbente, Ayixon Sánchez-Reyes, Alan D W Dobson, Jorge Luis Folch-Mallol
Extreme habitats have usually been regarded as a source of microorganisms that possess robust proteins that help enable them to survive in such harsh conditions. The deep sea can be considered an extreme habitat due to low temperatures (<5°C) and high pressure, however marine sponges survive in these habitats. While bacteria derived from deep-sea marine sponges have been studied, much less information is available on fungal biodiversity associated with these sponges. Following screening of fourteen fungi isolated from the deep-sea sponge Stelletta normani sampled at a depth of 751 metres, three halotolerant strains (TS2, TS11 and TS12) were identified which displayed high CMCase and xylanase activities...
2017: PloS One
https://www.readbyqxmd.com/read/28331163/in-vitro-evaluation-of-candidate-bacillus-spp-for-animal-feed
#2
Sirima Mingmongkolchai, Watanalai Panbangred
The aim of this study was to select aerobic spore-formers for animal feed based on their in vitro probiotic potential, including their enzyme-producing ability and safety assessment. Seven isolates out of 187 spore-forming bacteria were selected for their ability to produce cellulase (89.21-1668.32 U/ml), xylanase (1399.68-4351.10 U/ml), and phytase (2.72-28.70 U/ml). Among seven isolates, five had activities towards a broad range of p-nitrophenyl esters with acyl chain lengths from C2 to C12. The probiotic properties of all selected isolates varied with respect to their acid and bile salt tolerance under simulated gastrointestinal tract (GIT) conditions, and their adherence ability to human intestinal cell lines (Caco-2 and HT-29)...
March 22, 2017: Journal of General and Applied Microbiology
https://www.readbyqxmd.com/read/28330308/construction-of-aspergillus-niger-integrated-with-cellulase-gene-from-ampullaria-gigas-spix-for-improved-enzyme-production-and-saccharification-of-alkaline-pretreated-rice-straw
#3
Peizhou Yang, Haifeng Zhang, Lili Cao, Zhi Zheng, Shaotong Jiang
Aspergillus niger is an important microorganism that has been used for decades to produce extracellular enzymes. In this study, a novel Aspergillus niger strain integrated with a eukaryotic expression vector harboring the gpd-Shi promoter of shiitake mushrooms and cellulase gene of Ampullaria gigas Spix was engineered to improve cellulase production for the achievement of highly efficient saccharification of agricultural residues. In one strain, designated ACShi27, which exhibited the highest total cellulase expression, total cellulase, endoglucanase, exoglucanase, and xylanase expression levels were 1...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330264/construction-of-recombinant-sestc-saccharomyces-cerevisiae-for-consolidated-bioprocessing-cellulase-characterization-and-ethanol-production-by-in-situ-fermentation
#4
Peizhou Yang, Haifeng Zhang, Shaotong Jiang
Bioethanol is an important oil substitute produced by the sugar fermentation process. To improve the efficiency of cellulase expression of Saccharomyces cerevisiae, a eukaryotic expression vector harboring a single-enzyme-system-three-cellulase gene (sestc) was integrated into the S. cerevisiae genome by the protoplast method. Using PCR screening, RT-PCR, and "transparent circle" detection, several recombinant S. cerevisiae strains, capable of efficiently expressing the heterogeneous cellulase, were selected...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330241/purification-characterization-gene-cloning-and-expression-of-gh-10-xylanase-penicillium-citrinum-isolate-hzn13
#5
Zabin K Bagewadi, Sikandar I Mulla, Harichandra Z Ninnekar
An extracellular thermostable xylanase (Xyl-IIb) produced by Penicillium citrinum isolate HZN13 was purified to homogeneity using DEAE-Sepharose, Sephadex G-100 and Bio-Gel P-60 chromatography with specific activity of 6272.7 U/mg and 19.6-fold purification. The purification revealed the occurrence of multiple forms of xylanases (Xyl-I, Xyl-IIa, Xyl-IIb and Xyl-III). The molecular mass of highly purified Xyl-IIb was ~31 kDa with SDS-PAGE. The enzyme was cellulase-free, thermostable (55-75 °C) and acidophilic (3...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330237/biocatalytic-activity-of-aspergillus-niger-xylanase-in-paper-pulp-biobleaching
#6
A Sridevi, A Sandhya, G Ramanjaneyulu, G Narasimha, P Suvarnalatha Devi
Xylanase is a hemicellulase enzyme that catalyses the hydrolysis of xylan to xylose which is widely used in processing of feed, pulp and paper. It is produced by many microorganisms especially filamentous fungi like Trichoderma and Aspergillus. A potential xylanolytic fungal isolate Aspergillus niger was isolated from forest soils of Tirumala, AP, India, and its crude enzyme was checked for its potential in paper bleaching. Under submerged fermentation, production of xylanase, cellulase, biomass, total protein and sugar released were analysed after 7 days of incubation at room temperature...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330236/xylanase-production-from-penicillium-citrinum-isolate-hzn13-using-response-surface-methodology-and-characterization-of-immobilized-xylanase-on-glutaraldehyde-activated-calcium-alginate-beads
#7
Zabin K Bagewadi, Sikandar I Mulla, Yogesh Shouche, Harichandra Z Ninnekar
The present study reports the production of high-level cellulase-free xylanase from Penicillium citrinum isolate HZN13. The variability in xylanase titers was assessed under both solid-state (SSF) and submerged (SmF) fermentation. SSF was initially optimized with different agro-waste residues, among them sweet sorghum bagasse was found to be the best substrate that favored maximum xylanase production (9643 U/g). Plackett-Burman and response surface methodology employing central composite design were used to optimize the process parameters for the production of xylanase under SSF...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330222/bacterial-xylanases-biology-to-biotechnology
#8
REVIEW
Hillol Chakdar, Murugan Kumar, Kuppusamy Pandiyan, Arjun Singh, Karthikeyan Nanjappan, Prem Lal Kashyap, Alok Kumar Srivastava
In this review, a comprehensive discussion exclusively on bacterial xylanases; their gene organization; different factors and conditions affecting enzyme yield and activity; and their commercial application have been deliberated in the light of recent research findings and extensive information mining. Improved understanding of biological properties and genetics of bacterial xylanase will enable exploitation of these enzymes for many more ingenious biotechnological and industrial applications.
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330216/potential-application-of-waste-from-castor-bean-ricinus-communis-l-for-production-for-xylanase-of-interest-in-the-industry
#9
Polyanna Nunes Herculano, Keila Aparecida Moreira, Raquel Pedrosa Bezerra, Tatiana Souza Porto, Cristina Maria de Souza-Motta, Ana Lúcia Figueiredo Porto
Xylanases activity (XY) from Aspergillus japonicus URM5620 produced by Solid-State Fermentation (SSF) of castor press cake (Ricinus communis) on different conditions of production and extraction by PEG/citrate aqueous two-phase system (ATPS) were investigated. XY production was influenced by substrate amount (5-10 g), initial moisture (15-35 %), pH (4.0-6.0) and temperature (25-35 °C), obtaining the maximum activity of 29,085 ± 1808 U g ds(-1) using 5.0 g of substrate with initial moisture of 15 % at 25 °C and pH 6...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330199/optimization-of-fermentation-media-and-growth-conditions-for-microbial-xylanase-production
#10
Bushra Kalim, Nazish Mazhar Ali
Efficiency of cellulase-free xylanases is one of the determining factors in paper and pulp industries. Use of microbes which can produce cellulase-free xylanases may help to overcome the current challenges in kraft pulp processing. Isolation and screening of microorganisms from local samples offers a possibility for obtaining the potential microbes for this purpose. This research was therefore aimed to collect, screen, characterize and identify potential cellulase-free xylanase producers. A total of 313 microbial isolates were collected while using selective media (EBAM and XAM) to determine the xylanolytic potential of microbes...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330103/profiling-and-production-of-hemicellulases-by-thermophilic-fungus-malbranchea-flava-and-the-role-of-xylanases-in-improved-bioconversion-of-pretreated-lignocellulosics-to-ethanol
#11
Manju Sharma, Chhavi Mahajan, Manpreet S Bhatti, Bhupinder Singh Chadha
This study reports thermophilic fungus Malbranchea flava as a potent source of xylanase and xylan-debranching accessory enzymes. M. flava produced high levels of xylanase on sorghum straw containing solidified culture medium. The optimization of culture conditions for production of hemicellulases was carried out using one factor at a time approach and Box-Behnken design of experiments with casein (%), inoculum age (h) and inoculum level (ml) as process variables and xylanase, β-xylosidase, acetyl esterases and arabinofuranosidase as response variables...
June 2016: 3 Biotech
https://www.readbyqxmd.com/read/28323242/investigation-of-the-indigenous-fungal-community-populating-barley-grains-secretomes-and-xylanolytic-potential
#12
Abida Sultan, Jens C Frisvad, Birgit Andersen, Birte Svensson, Christine Finnie
The indigenous fungal species populating cereal grains produce numerous plant cell wall-degrading enzymes including xylanases, which could play important role in plant-pathogen interactions and in adaptation of the fungi to varying carbon sources. To gain more insight into the grain surface-associated enzyme activity, members of the populating fungal community were isolated, and their secretomes and xylanolytic activities assessed. Twenty-seven different fungal species were isolated from grains of six barley cultivars over different harvest years and growing sites...
March 17, 2017: Journal of Proteomics
https://www.readbyqxmd.com/read/28316134/feruloylation-and-structure-of-arabinoxylan-in-wheat-endosperm-cell-walls-from-rnai-lines-with-suppression-of-genes-responsible-for-backbone-synthesis-and-decoration
#13
Jackie Freeman, Jane L Ward, Ondrej Kosik, Alison Lovegrove, Mark D Wilkinson, Peter R Shewry, Rowan A C Mitchell
Arabinoxylan (AX) is the major component of the cell walls of wheat grain (70% in starchy endosperm) and is an important determinant of end-use qualities affecting food processing, use for animal feed and distilling and is a major source of dietary fibre in the human diet. AX is a heterogeneous polysaccharide composed of fractions which can be sequentially extracted by water (WE-AX), then xylanase action (XE-AX) leaving an unextractable (XU-AX) fraction. We determined arabinosylation and feruloylation of AX in these fractions in both wild-type wheat and RNAi lines with decreased AX content (TaGT43_2 RNAi, TaGT47_2 RNAi) or decreased arabinose 3-linked to mono-substituted xylose (TaXAT1 RNAi)...
March 17, 2017: Plant Biotechnology Journal
https://www.readbyqxmd.com/read/28315372/soybean-hull-induced-production-of-carbohydrases-and-protease-among-aspergillus-and-their-effectiveness-in-soy-flour-carbohydrate-and-protein-separation
#14
Qian Li, Abdullah Al Loman, Anthony M Coffman, Lu-Kwang Ju
Soybean hull consists mainly of three major plant carbohydrates, i.e., cellulose, hemicellulose and pectin. It is inexpensive and a good potential substrate for carbohydrase production because it is capable of inducing a complete spectrum of activities to hydrolyze complex biomass. Aspergillus is known for carbohydrase production but no studies have evaluated and compared, among Aspergillus species and strains, the soybean hull induced production of various carbohydrases. In this study, A. aculeatus, A. cinnamomeus, A...
March 14, 2017: Journal of Biotechnology
https://www.readbyqxmd.com/read/28300184/corrigendum-high-level-expression-of-improved-thermo-stable-alkaline-xylanase-variant-in-pichia-pastoris-through-codon-optimization-multiple-gene-insertion-and-high-density-fermentation
#15
Yihong Lu, Cheng Fang, Qinhong Wang, Yuling Zhou, Guimin Zhang, Yanhe Ma
No abstract text is available yet for this article.
March 16, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28298671/optimization-of-additive-content-and-their-combination-to-improve-the-quality-of-pure-barley-bread
#16
Milica Pojić, Tamara Dapčević Hadnađev, Miroslav Hadnađev, Slađana Rakita, Aleksandra Torbica
The objective of this study was to model the influence of pregelatinized OSA starch (OSA), wheat gluten (Gl) and xylanase (Xyl) on breadmaking potential of barley flour by using response surface methodology. Addition of these ingredients had significant effect on specific bread volume, crust and crumb lightness, crumb texture, average cell size and crumb density. OSA showed the most pronounced effect on specific bread volume, average cell size, crumb density and hardness. Interaction between OSA and Gl, as well as Gl and Xyl, respectively, increased and decreased the specific bread volume and crumb chewiness, while the interaction between OSA and Xyl decreased the specific volume decrease and increased crumb chewiness...
March 2017: Journal of Food Science and Technology
https://www.readbyqxmd.com/read/28286012/development-of-a-novel-ultrasound-assisted-alkali-pretreatment-strategy-for-the-production-of-bioethanol-and-xylanases-from-chili-post-harvest-residue
#17
Raveendran Sindhu, Parameswaran Binod, Anil Kuruvilla Mathew, Amith Abraham, Edgard Gnansounou, Sabeela Beevi Ummalyma, Leya Thomas, Ashok Pandey
A novel ultrasound-assisted alkali pretreatment strategy was developed which could effectively remove lignin and hemicelluloses and improve the sugar yield from chili post harvest residue. Operational parameters that affect the pretreatment efficiency were studied and optimized. Inhibitor analysis of the hydrolyzate revealed that major fermentation inhibitors like furfural, 5-hydroxymethyl furfural as well as organic acids like citric acid, succinic acid and propionic acid were absent. Hence fermentation can be carried out without detoxification of the hydrolyzate...
March 4, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28285179/expanding-the-feruloyl-esterase-gene-family-of-aspergillus-niger-by-characterization-of-a-feruloyl-esterase-faec
#18
Adiphol Dilokpimol, Miia R Mäkelä, Sadegh Mansouri, Olga Belova, Martin Waterstraat, Mirko Bunzel, Ronald P de Vries, Kristiina S Hildén
A feruloyl esterase (FAE) from Aspergillus niger N402, FaeC was heterologously produced in Pichia pastoris X-33 in a yield of 10mg/L. FaeC was most active at pH 7.0 and 50°C, and showed broad substrate specificity and catalyzed the hydrolysis of methyl 3,4-dimethoxycinnamate, ethyl ferulate, methyl ferulate, methyl p-coumarate, ethyl coumarate, methyl sinapate, and methyl caffeate. The enzyme released both ferulic acid and p-coumaric acid from wheat arabinoxylan and sugar beet pectin (up to 3mg/g polysaccharide), and acted synergistically with a commercial xylanase increasing the release of ferulic acid up to six-fold...
March 8, 2017: New Biotechnology
https://www.readbyqxmd.com/read/28284313/one-step-combined-focused-eppcr-and-saturation-mutagenesis-for-thermostability-evolution-of-a-new-cold-active-xylanase
#19
Juan Pablo Acevedo, Manfred T Reetz, Juan A Asenjo, Loreto P Parra
Enzymes active at low temperature are of great interest for industrial bioprocesses due to their high efficiency at a low energy cost. One of the particularities of naturally evolved cold-active enzymes is their increased enzymatic activity at low temperature, however the low thermostability presented in this type of enzymes is still a major drawback for their application in biocatalysis. Directed evolution of cold-adapted enzymes to a more thermostable version, appears as an attractive strategy to fulfill the stability and activity requirements for the industry...
May 2017: Enzyme and Microbial Technology
https://www.readbyqxmd.com/read/28270131/identification-of-two-transcription-factors-activating-the-expression-of-osxip-in-rice-defence-response
#20
Yihua Zhan, Xiangyu Sun, Guozeng Rong, Chunxiao Hou, Yingying Huang, Dean Jiang, Xiaoyan Weng
BACKGROUND: Xylanase inhibitors have been confirmed to be involved in plant defence. OsXIP is a XIP-type rice xylanase inhibitor, yet its transcriptional regulation remains unknown. RESULTS: Herbivore infestation, wounding and methyl jasmonate (MeJA) treatment enhanced mRNA levels and protein levels of OsXIP. By analyzing different 5' deletion mutants of OsXIP promoter exposed to rice brown planthopper Nilaparvata lugens stress, a 562 bp region (-1451 - -889) was finally identified as the key sequence for the herbivores stress response...
March 7, 2017: BMC Biotechnology
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