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https://www.readbyqxmd.com/read/28433165/efficiency-of-xylanases-from-families-10-and-11-in-production-of-xylo-oligosaccharides-from-wheat-arabinoxylans
#1
Natalie K Morgan, Andrew Wallace, Michael R Bedford, Mingan Choct
This study investigated the effect of xylanases from families 10 and 11 on the production of small chain arabinoxylo-oligosaccharides (AXOS) (X2-X4) from wheat arabinoxylans, and identified the impact that pH, temperature and time exposed to xylanase have on this process. Xylanase from family 10 had greater hydrolytic efficiency and resulted in heightened AXOS production compared with xylanase from family 11 or family 10 and 11 combined. The pH of the environment had a significant effect on AXOS production (P<0...
July 1, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/28433150/production-of-prebiotic-xylooligosaccharides-from-alkali-pretreated-mahogany-and-mango-wood-sawdust-by-using-purified-xylanase-of-clostridium-strain-boh3
#2
Gobinath Rajagopalan, Kavitha Shanmugavelu, Kun-Lin Yang
Xylooligosaccharides (XOS) are emerging prebiotics which can be produced from lignocellulosic biomass including agro-residues and hardwood. In this study, we report the production of XOS from thermal-alkali pretreated hardwood such as mahogany and mango by using a purified xylanase from Clostridium strain BOH3. In the first approach, pure xylan is extracted from mahogany and mango hardwood and then the pure xylan is hydrolyzed by using the xylanase. In this case, 572 and 504mg XOS/g pure xylan were obtained from mahogany and mango woods, respectively...
July 1, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/28432102/two-distinct-%C3%AE-l-arabinofuranosidases-in-caldicellulosiruptor-species-drive-degradation-of-arabinose-based-polysaccharides
#3
Md Abu Saleh, Wen-Jie Han, Ming Lu, Bing Wang, Huayue Li, Robert M Kelly, Fu-Li Li
Species in the extremely thermophilic genus Caldicellulosiruptor can degrade unpretreated plant biomass through the action of multi-modular glycoside hydrolases. To date, most focus with these bacteria has been on hydrolysis of glucans and xylans, while biodegradation mechanism for arabinose-based polysaccharides remains unclear. Here, putative α-L-arabinofuranosidases (AbFs) were identified in Caldicellulosiruptor species by homology to less thermophilic versions of these enzymes. From this screen, an extracellular XynF was determined to be a key factor in hydrolyzing α-1,2-, α-1,3-, and α-1,5-L-arabinofuranosyl residues of arabinose-based polysaccharides...
April 21, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/28431149/next-generation-non-starch-polysaccharide-degrading-multi-carbohydrase-complex-rich-in-xylanase-and-arabinofuranosidase-to-enhance-broiler-feed-digestibility
#4
Pierre Cozannet, Michael T Kidd, Roberto Montanhini Neto, Pierre-André Geraert
This study was carried out to evaluate the effect of a multi-carbohydrase complex ( ) rich in xylanase ( ) and arabinofuranosidase ( ) on overall broiler feed digestibility in broilers. Energy utilization and digestibility of dry matter ( ), organic matter ( ), protein, starch, fat, and insoluble and soluble fibers were measured using the mass-balance method. The experiment was carried out on 120 broilers (3-week-old chickens). Broilers were distributed over 8 treatments to evaluate the effect of the dietary arabinoxylan content and nutrient density with and without MCC (Rovabio® Advance)...
April 20, 2017: Poultry Science
https://www.readbyqxmd.com/read/28431146/response-of-broiler-chickens-fed-wheat-based-diets-to-xylanase-supplementation
#5
G Gonzalez-Ortiz, D Sola-Oriol, M Martinez-Mora, J F Perez, M R Bedford
The objective of this study was to evaluate the effect of xylanase supplementation on performance, footpad score (FPS), nutrient digestibility, and intestinal morphometry in broiler chickens. Two-hundred-eighty-eight Ross 308 broiler chicks (one d old) were placed in one of 3 experimental treatments: positive control (PC), negative control (NC) (-150 kcal/kg), and XYL (NC supplemented with xylanase). Each treatment had 8 replicates with 12 animals each. Starter (zero to 21 d) and grower (21 to 42 d) diets, based on wheat and soy-bean meal, were available ad libitum...
April 18, 2017: Poultry Science
https://www.readbyqxmd.com/read/28428823/combining-manipulation-of-transcription-factors-and-overexpression-of-the-target-genes-to-enhance-lignocellulolytic-enzyme-production-in-penicillium-oxalicum
#6
Liwei Gao, Zhonghai Li, Chengqiang Xia, Yinbo Qu, Meng Liu, Piao Yang, Lele Yu, Xin Song
BACKGROUND: Lignocellulolytic enzymes are the main enzymes to saccharify lignocellulose from renewable plant biomass in the bio-based economy. The production of these enzymes is transcriptionally regulated by multiple transcription factors. We previously engineered Penicillium oxalicum for improved cellulase production via manipulation of three genes in the cellulase expression regulatory network. However, the potential of combinational engineering of multiple regulators and their targets at protein abundance and activity levels has not been fully explored...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/28419372/nutrient-and-fiber-utilization-responses-of-supplemental-xylanase-in-broiler-chickens-fed-wheat-based-diets-are-independent-of-the-adaptation-period-to-test-diets2
#7
E Kiarie, M C Walsh, L F Romero, S Arent, V Ravindran
The effects of adaptation (AD) to xylanase-supplemented diets on nutrient and fiber utilization in 21-d-old broilers were investigated. Six treatments, arranged in two levels of AD (starting at d 0 or d 14 of age) and three levels of xylanase (0 or 2,500 or 5,000 xylanase units/kg feed) were used. All diets had 500 phytase U/kg and 0.3% TiO2 as indigestible marker. A total of 384 d old male broiler (Ross 308) chicks were divided into two groups. The first group was assigned on weight basis to 24 cages (8 chicks per cage) and randomly allocated to the diets from d 0...
April 17, 2017: Poultry Science
https://www.readbyqxmd.com/read/28413445/valorization-of-untreated-rice-bran-towards-bioflocculant-using-a-lignocellulose-degrading-strain-and-its-use-in-microalgal-biomass-harvest
#8
Cong Liu, Yan Hao, Jihong Jiang, Weijie Liu
BACKGROUND: Microalgae are currently considered as a promising feedstock for the production of biofuels and high-value products. However, the efficient harvest of microalgal biomasses from their culture broth is a major challenge. The harvesting of algal biomass by flocculation combined with gravity sedimentation is more convenient and cost-effective than traditional methods such as centrifugation and filtration. Compared to inorganic and chemically synthetic flocculants, bioflocculants are a suitable choice for microalgal harvest due to their biodegradable and nontoxic properties...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/28412647/ecofriendly-lignocellulose-pretreatment-to-enhance-the-carboxylate-production-of-a-rumen-derived-microbial-consortium
#9
Adèle Lazuka, Cécile Roland, Abdellatif Barakat, Fabienne Guillon, Michael O'Donohue, Guillermina Hernandez-Raquet
Innovative dry chemo- and chemo-mechanical pretreatments form an interesting approach for modifying the native physico-chemical composition of lignocellulose facilitating its microbial conversion to carboxylates. Here, the impact of four dry-pretreatment conditions on the microbial transformation of wheat straw was assessed: milling to 2mm and 100µm, and NaOH chemical impregnation at high substrate concentrations combined with milling at 2mm and 100µm. Pretreatment effect was assessed in the light of substrate structure and composition, its impact on the acidogenic potential and the major enzyme activities of a rumen-derived microbial consortium RWS...
March 16, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28409383/functional-analysis-of-arabinofuranosidases-and-a-xylanase-of-corynebacterium-alkanolyticum-for-arabinoxylan-utilization-in-corynebacterium-glutamicum
#10
Takayuki Kuge, Akira Watanabe, Satoshi Hasegawa, Haruhiko Teramoto, Masayuki Inui
Xylooligosaccharides (XOSs) and arabinoxylooligosaccharides (AXOSs) are major oligosaccharides derived from arabinoxylan. In our previous report, Corynebacterium glutamicum was engineered to utilize XOSs by introducing Corynebacterium alkanolyticum xyloside transporter and β-xylosidase. However, this strain was unable to consume AXOSs due to the absence of α-L-arabinofuranosidase activity. In this study, to confer AXOS utilization ability on C. glutamicum, two putative arabinofuranosidase genes (abf51A and abf51B) were isolated from C...
April 13, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28402035/screening-and-investigation-lactobacillius-spp-to-improve-secale-cereale-silage-quality
#11
Hye Sun Kim, Ouk Kyu Han, Sam Churl Kim, Min Jung Kim, Youn-Sig Kwak
Silage is a high-moisture-content forage that is used to feed livestock. Using silage as feed is economically feasible and suitable for cattle management. Secale cereale is considered as a high-quality forage plant. After the heading stage the biomass of S. cereale increases by up to 30%; however, lignification in the cell wall causes low fermentation efficiency and coefficient digestibility, and it may also be contaminated by mycotoxin-producing fungi and can impair the quality of the silage. Therefore, the aim of this study was to screen beneficial Lactobacillius spp...
April 12, 2017: Animal Science Journal, Nihon Chikusan Gakkaihō
https://www.readbyqxmd.com/read/28401458/purification-characterization-and-thermostability-improvement-of-xylanase-from-bacillus-amyloliquefaciens-and-its-application-in-pre-bleaching-of-kraft-pulp
#12
Sharad Kumar, Izharul Haq, Jyoti Prakash, Sudheer Kumar Singh, Shivaker Mishra, Abhay Raj
Xylanases have important industrial applications but are most extensively utilized in the pulp and paper industry as a pre-bleaching agent. We characterized a xylanase from Bacillus amyloliquefaciens strain SK-3 and studied it for kraft pulp bleaching. The purified enzyme had a molecular weight of ~50 kDa with optimal activity at pH 9.0 and 50 °C. The enzyme showed good activity retention (85%) after 2 h incubation at 50 °C and pH 9.0. This enzyme obeyed Michaelis-Menten kinetics with regard to beechwood xylan with K m and V max values of 5...
May 2017: 3 Biotech
https://www.readbyqxmd.com/read/28397123/synergetic-effect-of-rhamnolipid-from-pseudomonas-aeruginosa-c1501-and-phytotoxic-metabolite-from-lasiodiplodia-pseudotheobromae-c1136-on-amaranthus-hybridus-l-and-echinochloa-crus-galli-weeds
#13
Charles Adetunji, Julius Oloke, Anil Kumar, Singh Swaranjit, Benjamin Akpor
Rhamnolipid (Rh) is a biosurfactant produced by the bacterial Pseudomonas aeruginosa. This present study investigates rhizospheric strain C1501 of P. aeruginosa with an accession number KF976394 with the best production of rhamnolipid: a biosurfactant. The partially purified rhamnolipid from strain C1501 and Tween 80 was tested on mycelial growth of wild strain C1136. The enzyme activities involved in biodegradation, as well as necrosis induction on the tested weeds, were performed using scanning electron microscopy...
April 10, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28393500/enhanced-direct-ethanol-production-by-cofactor-optimization-of-cell-surface-displayed-xylose-isomerase-in-yeast
#14
Yusuke Sasaki, Toshiyuki Takagi, Keisuke Motone, Kouichi Kuroda, Mitsuyoshi Ueda
Xylose isomerase (XylC) from Clostridium cellulovorans can simultaneously perform isomerization and fermentation of d-xylose, the main component of lignocellulosic biomass, and is an attractive candidate enzyme. In this study, we optimized a specified metal cation in a previously established Saccharomyces cerevisiae strain displaying XylC. We investigated the effect of each metal cation on the catalytic function of the XylC-displaying S. cerevisiae. Results showed that the divalent cobalt cations (Co(2+) ), especially enhanced the activity by 46-fold...
April 10, 2017: Biotechnology Progress
https://www.readbyqxmd.com/read/28391478/concurrent-production-of-cellulase-and-xylanase%C3%A2-from%C3%A2-trichoderma-reesei%C3%A2-ncim-1186-%C3%A2-enhancement-of-production-by%C3%A2-desirability-based-multi-objective-method
#15
Preethi Jampala, Satish Tadikamalla, M Preethi, Swathy Ramanujam, Kiran Babu Uppuluri
Application of multiple response optimizations using desirability function in the production of microbial metabolites improves economy and efficiency. Concurrent production of cellulase and xylanase in Trichoderma reesei NCIM 1186 using an agricultural weed, Prosopis juliflora pods, was studied. The main aim of the study was to optimize significant medium nutrient parameters for maximization of cellulase and xylanase by multi-objective optimization strategy using biomass. Process parameters such as the nutrient concentrations (pods, sucrose, and yeast extract) and pH were investigated to improve cellulase and xylanase activities by one factor at a time approach, single response optimization and multi-objective optimization...
May 2017: 3 Biotech
https://www.readbyqxmd.com/read/28391477/microbial-xylanases-and-their-industrial-application-in-pulp-and-paper-biobleaching-a-review
#16
Abhishek Walia, Shiwani Guleria, Preeti Mehta, Anjali Chauhan, Jyoti Parkash
Xylanases are hydrolytic enzymes which cleave the β-1, 4 backbone of the complex plant cell wall polysaccharide xylan. Xylan is the major hemicellulosic constituent found in soft and hard food. It is the next most abundant renewable polysaccharide after cellulose. Xylanases and associated debranching enzymes produced by a variety of microorganisms including bacteria, actinomycetes, yeast and fungi bring hydrolysis of hemicelluloses. Despite thorough knowledge of microbial xylanolytic systems, further studies are required to achieve a complete understanding of the mechanism of xylan degradation by xylanases produced by microorganisms and their promising use in pulp biobleaching...
May 2017: 3 Biotech
https://www.readbyqxmd.com/read/28389896/improved-efficacy-of-ultrafiltered-xylanase-pectinase-concoction-in-biobleaching-of-plywood-waste-soda-pulp
#17
Divya Sharma, Sharad Agrawal, Ravi Dutt Yadav, Ritu Mahajan
The effect of ultrafiltered xylanase-pectinase concoction produced simultaneously by a bacterial isolate using agro-waste-based media was assessed in prebleaching of plywood waste pulp. Ultrafiltered enzymes caused 12.5% reduction in kappa number at reduced enzyme dose of xylanase-pectinase (4.0-0.8 IU) per gram of pulp under optimized conditions at pH 8.5, temperature 55 °C, and treatment period of 2 h. Using this methodology, amount of Cl2-ClO2 consumption can be reduced up to 30 and 28.86%. Significant improvement in physical and optical properties of pulp was obtained along with an additional reduction in BOD and COD values up to 18...
May 2017: 3 Biotech
https://www.readbyqxmd.com/read/28386674/microplate-based-evaluation-of-the-sugar-yield-from-giant-reed-giant-miscanthus-and-switchgrass-after-mild-chemical-pre-treatments-and-hydrolysis-with-tailored-trichoderma-enzymatic-blends
#18
Stefano Cianchetta, Luca Bregoli, Stefania Galletti
Giant reed, miscanthus, and switchgrass are considered prominent lignocellulosic feedstocks to obtain fermentable sugars for biofuel production. The bioconversion into sugars requires a delignifying pre-treatment step followed by hydrolysis with cellulase and other accessory enzymes like xylanase, especially in the case of alkali pre-treatments, which retain the hemicellulose fraction. Blends richer in accessory enzymes than commercial mix can be obtained growing fungi on feedstock-based substrates, thus ten selected Trichoderma isolates, including the hypercellulolytic strain Trichoderma reesei Rut-C30, were grown on giant reed, miscanthus, or switchgrass-based substrates...
April 6, 2017: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/28384512/novel-substrates-for-the-automated-and-manual-assay-of-endo-1-4-%C3%AE-xylanase
#19
David Mangan, Claudio Cornaggia, Agnija Liadova, Niall McCormack, Ruth Ivory, Vincent A McKie, Aaron Ormerod, Barry V McCleary
endo-1,4-β-Xylanase (EC 3.2.1.8) is employed across a broad range of industries including animal feed, brewing, baking, biofuels, detergents and pulp (paper). Despite its importance, a rapid, reliable, reproducible, automatable assay for this enzyme that is based on the use of a chemically defined substrate has not been described to date. Reported herein is a new enzyme coupled assay procedure, termed the XylX6 assay, that employs a novel substrate, namely 4,6-O-(3-ketobutylidene)-4-nitrophenyl-β-4(5)-O-glucosyl-xylopentaoside...
March 4, 2017: Carbohydrate Research
https://www.readbyqxmd.com/read/28377764/carbohydrate-staple-food-modulates-gut-microbiota-of-mongolians-in-china
#20
Jing Li, Qiangchuan Hou, Jiachao Zhang, Haiyan Xu, Zhihong Sun, Bilige Menghe, Heping Zhang
Gut microbiota is a determining factor in human physiological functions and health. It is commonly accepted that diet has a major influence on the gut microbial community, however, the effects of diet is not fully understood. The typical Mongolian diet is characterized by high and frequent consumption of fermented dairy products and red meat, and low level of carbohydrates. In this study, the gut microbiota profile of 26 Mongolians whom consumed wheat, rice and oat as the sole carbohydrate staple food for a week each consecutively was determined...
2017: Frontiers in Microbiology
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