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Xylanase

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https://www.readbyqxmd.com/read/28741666/assessment-of-the-potential-of-the-multi-enzyme-producer-bacillus-amyloliquefaciens-us573-as-alternative-feed-additive
#1
Ameny Farhat-Khemakhem, Monia Blibech, Ines Boukhris, Mohamed Makni, Hichem Chouayekh
BACKGROUND: Recently, probiotics have increasingly been used as feed additives in poultry diets as an alternative to antibiotic growth promoters fostering resistance development. RESULTS: This study was aimed at assessing the potential of Bacillus amyloliquefaciens US573 as a direct-fed microbial. The US573 strain was found to be free of harmful enzymatic activities and sensitive to antibiotics. In addition, it showed a good acid and bovine bile tolerance, high adhesion efficacy to chicken enterocytes, and an ability to form biofilms, which may favor its survival and persistence in the animal gastrointestinal tract...
July 25, 2017: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/28738544/a-xylanase-aided-enzymatic-pretreatment-facilitates-cellulose-nanofibrillation
#2
Lingfeng Long, Dong Tian, Jinguang Hu, Fei Wang, Jack Saddler
Although biological pretreatment of cellulosic fiber based on endoglucanases has shown some promise to facilitate cellulose nanofibrillation, its efficacy is still limited. In this study, a xylanase-aided endoglucanase pretreatment was assessed on the bleached hardwood and softwood Kraft pulps to facilitate the downstream cellulose nanofibrillation. Four commercial xylanase preparations were compared and the changes of major fiber physicochemical characteristics such as cellulose/hemicellulose content, gross fiber properties, fiber morphologies, cellulose accessibility/degree of polymerization (DP)/crystallinity were systematically evaluated before and after enzymatic pretreatment...
July 9, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28736694/biochemical-properties-of-a-new-thermo-and-solvent-stable-xylanase-recovered-using-three-phase-partitioning-from-the-extract-of-bacillus-oceanisediminis-strain-sj3
#3
Nawel Boucherba, Mohammed Gagaoua, Amel Bouanane-Darenfed, Cilia Bouiche, Khelifa Bouacem, Mohamed Yacine Kerbous, Yacine Maafa, Said Benallaoua
The present study investigates the production and partial biochemical characterization of an extracellular thermostable xylanase from the Bacillus oceanisediminis strain SJ3 newly recovered from Algerian soil using three phase partitioning (TPP). The maximum xylanase activity recorded after 2 days of incubation at 37 °C was 20.24 U/ml in the presence of oat spelt xylan. The results indicated that the enzyme recovered in the middle phase of TPP system using the optimum parameters were determined as 50% ammonium sulfate saturation with 1...
2017: Bioresources and Bioprocessing
https://www.readbyqxmd.com/read/28732724/recombinant-bacillus-amyloliquefaciens-xylanase-a-expressed-in-pichia-pastoris-and-generation-of-xylooligosaccharides-from-xylans-and-wheat-bran
#4
Ming-Qi Liu, Wen-Kang Huo, Xin Xu, Xiao-Yan Weng
In this study, BaxA (GenBank: KM624029), which encodes the Bacillus amyloliquefaciens xylanase A (BaxA), was highly expressed in Pichia pastoris GS115 under the control of the AOX1 promoter. The recombinant xylanase, namely rePBaxA, was purified to homogeneity by using Ni-affinity resin and its molecular weight was 35.0kDa. The optimum temperature and pH of rePBaxA were 50°C and 5.0, respectively. The kinetic parameters Michaelis-Menten constant (Km) and maximum reaction rate (Vmax) of rePBaxA were 5.41mg/mL and 22...
July 18, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28728936/cell-free-pipeline-for-discovery-of-thermotolerant-xylanases-and-endo-1-4-%C3%AE-glucanases
#5
Dejan Gagoski, Zhenyu Shi, Lars Keld Nielsen, Claudia E Vickers, Stephen Mahler, Robert Speight, Wayne A Johnston, Kirill Alexandrov
The rapid expansion in the number of sequenced genomes and metagenomes provides an exceptional resource for mining of the enzymes with biotechnologically relevant properties. However, the majority of protein production and analysis methods are not sufficiently cost-efficient and scalable to experimentally verify the results of computational genomic mining. Here, we present a pipeline based on Leishmania tarentolae cell-free system that was used to characterise 30 putative thermostable endo-1,4-β-glucanases and xylanases identified in public genomic databases...
July 17, 2017: Journal of Biotechnology
https://www.readbyqxmd.com/read/28726354/high-performance-of-agaricus-blazei-fungus-for-the-biological-pre-treatment-of-elephant-grass
#6
Thais Dal Picolli, Kaliane Regalin Aver, Roselei Claudete Fontana, Marli Camassola
Biological pre-treatment seems to be promising being an eco-friendly process, with no inhibitor generated during the process. The potential for elephant grass pre-treatment with white degradation fungi Pleurotus ostreatus, Agaricus blazei, Lentinula edodes, Pleurotus citrinopileatus and Pleurotus djamor, in isolated or mixed cultures of these strains, was evaluated. The highest activities of enzymes involved in the degradation of lignocellulosic biomass (laccases, endoglucanases, xylanases and β-glucosidases) were observed for A...
July 20, 2017: Biotechnology Progress
https://www.readbyqxmd.com/read/28725557/genome-assembly-of-chryseobacterium-sp-strain-ihbb-10212-from-glacier-top-surface-soil-in-the-indian-trans-himalayas-with-potential-for-hydrolytic-enzymes
#7
Mohinder Pal, Mohit Kumar Swarnkar, Hena Dhar, Sanjay Chhibber, Arvind Gulati
The cold-active esterases are gaining importance due to their catalytic activities finding applications in chemical industry, food processes and detergent industry as additives, and organic synthesis of unstable compounds as catalysts. In the present study, the complete genome sequence of 4,843,645 bp with an average 34.08% G + C content and 4260 protein-coding genes are reported for the low temperature-active esterase-producing novel strain of Chrysobacterium isolated from the top-surface soil of a glacier in the cold deserts of the Indian trans-Himalayas...
September 2017: Genomics Data
https://www.readbyqxmd.com/read/28720949/rheological-properties-and-microstructure-of-xylanase-containing-whole-wheat-bread-dough
#8
G Ghoshal, U S Shivhare, U C Banerjee
The present research work was undertaken to investigate the effect of xylanase, produced by Penicillium citrinum, on rheological behavior of whole wheat bread dough at large and small deformation respectively. Dough attributes including textural properties (penetration) and structure related characteristics (oscillatory tests) were evaluated. Change in visco-elastic properties of xylanase containing dough was evaluated by oscillatory and creep measurements. The flow experiments were conducted under steady-state condition with shear rate ranging from 0...
June 2017: Journal of Food Science and Technology
https://www.readbyqxmd.com/read/28713028/the-effect-of-fusarium-culmorum-infection-and-deoxynivalenol-don-application-on-proteome-response-in-barley-cultivars-chevron-and-pedant
#9
Klára Kosová, Jana Chrpová, Jiří Šantrůček, Radovan Hynek, Lenka Štěrbová, Pavel Vítámvás, Jana Bradová, Ilja Tom Prášil
Fusarium head blight (FHB) disease adversely affects grain quality and final yield in small-grain cereals including barley. In the present study, the effect of an artificial infection with Fusarium culmorum and an application of deoxynivalenol (DON) on barley spikes of cultivars Chevron and Pedant during flowering was investigated at grain mid-dough stage (BBCH 73) 10days after pathogen inoculation (10 dai). Proteomic analysis using a two-dimensional differential gel electrophoresis (2D-DIGE) technique coupled with LC-MS/MS investigated 98 protein spots revealing quantitative or qualitative differences between the experimental variants...
July 13, 2017: Journal of Proteomics
https://www.readbyqxmd.com/read/28711616/isolation-screening-and-characterization-of-a-novel-extracellular-xylanase-from-aspergillus-niger-kp874102-1-and-its-application-in-orange-peel-hydrolysis
#10
Uma Shankar Prasad Uday, Ria Majumdar, Onkar Nath Tiwari, Umesh Mishra, Abhijit Mondal, Tarun Kanti Bandyopadhyay, Biswanath Bhunia
In the present work, a potent xylanase producing fungal strain Aspergillus niger (KP874102.1) was isolated through cultural and morphological observations from soil sample of Baramura forest, Tripura west, India. 28S rDNA technique was applied for genomic identification of this fungal strain. The isolated strain was found to be phylogenetically closely related to Aspergillus niger. Kinetic constants such as Km and Vmax for extracellular xylanase were determined using various substrate such as beech wood xylan, oat spelt xylan and CM cellulose through Lineweaver-Burk plot...
July 12, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28704553/screening-and-characterizing-of-xylanolytic-and-xylose-fermenting-yeasts-isolated-from-the-wood-feeding-termite-reticulitermes-chinensis
#11
Sameh Samir Ali, Jian Wu, Rongrong Xie, Feng Zhou, Jianzhong Sun, Miao Huang
The effective fermentation of xylose remains an intractable challenge in bioethanol industry. The relevant xylanase enzyme is also in a high demand from industry for several biotechnological applications that inevitably in recent times led to many efforts for screening some novel microorganisms for better xylanase production and fermentation performance. Recently, it seems that wood-feeding termites can truly be considered as highly efficient natural bioreactors. The highly specialized gut systems of such insects are not yet fully realized, particularly, in xylose fermentation and xylanase production to advance industrial bioethanol technology as well as industrial applications of xylanases...
2017: PloS One
https://www.readbyqxmd.com/read/28698996/genome-sequencing-of-strain-cellulosimicrobium-sp-th-20-with-ginseng-biotransformation-ability
#12
REVIEW
Fei Zheng, Wei Zhang, Xiaodan Chu, Yulin Dai, Jing Li, Huanxi Zhao, Liankui Wen, Hao Yue, Shanshan Yu
Biotransformation for increasing the pharmaceutical effect of ginsenosides is getting more and more attractions. Strain Cellulosimicrobium sp. TH-20 isolated from ginseng soil samples was identified to produce enzymes contributing to its excellent biotransformation activity against ginsenosides, the main active components of ginseng. Based on phylogenetic tree and homology analysis, the strain can be designated as Cellulosimicrobium sp. Genome sequencing was performed using the Illumina Miseq to explore the functional genes involved in ginsenoside transformation...
August 2017: 3 Biotech
https://www.readbyqxmd.com/read/28693992/characterization-of-a-recombinant-xylose-tolerant-%C3%AE-xylosidase-from-humicola-grisea-var-thermoidea-and-its-use-in-sugarcane-bagasse-hydrolysis
#13
Lorena Cardoso Cintra, Amanda Gregorim Fernandes, Izadora Cristina Moreira de Oliveira, Saulo J Linhares Siqueira, Ilítia Ganaê Oliveira Costa, Francieli Colussi, Rosália Santos Amorim Jesuíno, Cirano José Ulhoa, Fabrícia Paula de Faria
One full-length β-xylosidase gene (hxylA) was identified from the Humicola grisea var. thermoidea genome and the cDNA was successfully expressed by Pichia pastoris SMD1168. An optimization of enzyme production was carried out, and methanol was found to be the most important parameter. The purified enzyme was characterized and showed the optimal conditions for the highest activity at pH 7.0 and 50°C, being thermostable by maintaining 41% of its activity after 12h incubated at 50°C. HXYLA is a bifunctional enzyme; it showed both β-xylosidase and α-arabinfuranosidase activities...
July 7, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28676961/production-of-high-commercial-value-xylooligosaccharides-from-meranti-wood-sawdust-using-immobilised-xylanase
#14
Siti Sabrina Mohd Sukri, A M Mimi Sakinah
The present study explores the utilisation of a new raw material from lignocellulose biomass, Meranti wood sawdust (MWS) for high commercial value xylooligosaccharides (XOS) production using immobilised xylanase. The xylanase was immobilised by a combination of entrapment and covalent binding techniques. The hemicellulosic xylan from MWS was extracted using a standard chlorite delignification method. The production of total and derivatives of XOS from the degradation of the hemicellulosic xylan of MWS were compared to the production from the commercial xylan from Beechwood...
July 4, 2017: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/28674832/heterologous-expression-and-characterization-of-an-acidic-gh11-family-xylanase-from-hypocrea-orientalis
#15
Hailong Li, Hongli Wu, Fengjiao Jiang, Jinlian Wu, Yong Xue, Lihui Gan, Jian Liu, Minnan Long
A gene encoding glycoside hydrolase family 11 xylanase (HoXyn11B) from Hypocrea orientalis EU7-22 was expressed in Pichia pastoris with a high activity (413 IU/ml). HoXyn11B was partly N-glycosylated and appeared two protein bands (19-29 kDa) on SDS-PAGE. The recombinant enzyme exhibited optimal activity at pH 4.5 and 55 °C, and retained more than 90% of the original activity after incubation at 50 °C for 60 min. The determined apparent K m and V max values using beechwood xylan were 10.43 mg/ml and 3246...
July 3, 2017: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/28673305/oligomerization-triggered-by-foldon-a-simple-method-to-enhance-the-catalytic-efficiency-of-lichenase-and-xylanase
#16
Xinzhe Wang, Huihua Ge, Dandan Zhang, Shuyu Wu, Guangya Zhang
BACKGROUND: Effective and simple methods that lead to higher enzymatic efficiencies are highly sough. Here we proposed a foldon-triggered trimerization of the target enzymes with significantly improved catalytic performances by fusing a foldon domain at the C-terminus of the enzymes via elastin-like polypeptides (ELPs). The foldon domain comprises 27 residues and can forms trimers with high stability. RESULTS: Lichenase and xylanase can hydrolyze lichenan and xylan to produce value added products and biofuels, and they have great potentials as biotechnological tools in various industrial applications...
July 3, 2017: BMC Biotechnology
https://www.readbyqxmd.com/read/28669823/biochemical-and-structural-analyses-of-two-cryptic-esterases-in-bacteroides-intestinalis-and-their-synergistic-activities-with-cognate-xylanases
#17
Daniel Wefers, Janaina J V Cavalcante, Rachel R Schendel, Jaigeeth Deveryshetty, Kui Wang, Zdzislaw Wawrzak, Roderick I Mackie, Nicole M Koropatkin, Isaac Cann
Arabinoxylans are constituents of the human diet. Although not utilizable by the human host, they can be fermented by colonic bacteria. The arabinoxylan backbone is decorated with arabinose side-chains that may be substituted with ferulic acid, thus limiting depolymerization to fermentable sugars. We investigated the polypeptides encoded by two genes upregulated during growth of the colonic bacterium Bacteroides intestinalis on wheat arabinoxylan. The recombinant proteins, designated BiFae1A and BiFae1B, were functionally assigned esterase activities...
June 29, 2017: Journal of Molecular Biology
https://www.readbyqxmd.com/read/28660507/raw-sugarcane-bagasse-as-carbon-source-for-xylanase-production-by-paenibacillus-species-a-potential-degrader-of-agricultural-wastes
#18
Enzo Di Marco, Pablo M Soraire, Cintia M Romero, Liliana B Villegas, María Alejandra Martínez
Paenibacillus species isolated from a variety of natural sources have shown to be important glycoside hydrolases producers. These enzymes play a key role in bio-refining applications, as they are central biocatalysts for the processing of different types of polymers from vegetal biomass. Xylanase production by three native isolates belonging to the genus Paenibacillus was approached by utilizing mineral-based medium and agricultural by-products as a convenient source to produce biocatalysts suitable for their degradation...
June 28, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28660456/sorghum-husk-biomass-as-a-potential-substrate-for-production-of-cellulolytic-and-xylanolytic-enzymes-by-nocardiopsis-sp-knu
#19
Siddheshwar D Kshirsagar, Bhumika N Bhalkar, Pankajkumar R Waghmare, Ganesh D Saratale, Rijuta G Saratale, Sanjay P Govindwar
Nocardiopsis sp. KNU was found to degrade various lignocellulosic waste materials, namely, sorghum husk, sugarcane tops and leaves, wheat straw, and rice husk very efficiently. The strain was found to produce high amounts of cellulase and hemicellulase. Augmentation of cotton seed cake as an organic nitrogen source revealed inductions in activities of endoglucanase, glucoamylase, and xylanase up to 70.03, 447.89, and 275.10 U/ml, respectively. Nonionic surfactant Tween-80 addition was found to enhance the activity of endoglucanase enzyme...
July 2017: 3 Biotech
https://www.readbyqxmd.com/read/28659894/thyme-oil-reduces-biofilm-formation-and-impairs-virulence-of-xanthomonas-oryzae
#20
Akanksha Singh, Rupali Gupta, Sudeep Tandon, Rakesh Pandey
Xanthomonas oryzae pv. oryzae (Xoo), a common bacterial plant pathogen regulates its virulence and biofilm formation attribute via a chemical method of communication. Disabling this mechanism offers a promising alternative to reduce the virulence and pathogencity of the microorganism. In this study, the effect of thyme (THY) oil on Quorum Sensing mediated synthesis of various virulence factors and biofilm formation was analyzed. Treatment of Xoo with 500 ppm THY oil displayed a significant diminution in swimming, swarming, exopolysaccharide and xanthomonadin secretion...
2017: Frontiers in Microbiology
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