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claudin gene regulation

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https://www.readbyqxmd.com/read/28279791/methionine-hydroxy-analogue-improves-intestinal-immunological-and-physical-barrier-function-in-young-grass-carp-ctenopharyngodon-idella
#1
Fei-Yu Pan, Pei Wu, Lin Feng, Wei-Dan Jiang, Sheng-Yao Kuang, Ling Tang, Wu-Neng Tang, Yong-An Zhang, Xiao-Qiu Zhou, Yang Liu
This study was conducted to test the hypothesis that methionine hydroxy analogue (MHA) enhances the defense against enteritis occurrence via improving intestinal barrier function in fish. After 630 young grass carp (Ctenopharyngodon idella) (259.70 ± 0.47 g) fed six graded levels of MHA (0, 2.4, 4.4, 6.4, 8.5 and 10.5 g/kg diet) and one dl-methionine group (6.4 g/kg diet) for 8 weeks. At the end of feeding trial, 15 fish from each treatment were challenged with Aeromonas hydrophila for 14 days. The results indicated that optimal MHA enhanced the capacity of fish against enteritis emergence, which might be related to the positive effects of MHA on intestinal immunological and physical barrier function in fish...
March 6, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28219650/dendritic-cells-death-induced-by-contact-sensitizers-is-controlled-by-nrf2-and-depends-on-glutathione-levels
#2
Zeina El Ali, Claudine Deloménie, Jérémie Botton, Marc Pallardy, Saadia Kerdine-Römer
Dendritic cells (DC) are known to play a major role during contact allergy induced by contact sensitizers (CS). Our previous studies showed that Nrf2 was induced in DC and controlled allergic skin inflammation in mice in response to chemicals. In this work, we raised the question of the role of Nrf2 in response to a stress provoked by chemical sensitizers in DC. We used two well-described chemical sensitizers, dinitrochlorobenzene (DNCB) and cinnamaldehyde (CinA), known to have different chemical reactivity and mechanism of action...
February 20, 2017: Toxicology and Applied Pharmacology
https://www.readbyqxmd.com/read/28216386/serotonin-disturbs-colon-epithelial-tolerance-of-commensal-e-coli-by-increasing-nox2-derived-superoxide
#3
Suhrid Banskota, Sushil Chandra Regmi, Jaya Gautam, Pallavi Gurung, Yu-Jeong Lee, Sae Kwang Ku, Jin-Hyung Lee, Jintae Lee, Hyeun Wook Chang, Sang Joon Park, Jung-Ae Kim
Adherent-invasive E. coli colonization and Toll-like receptor (TLR) expression are increased in the gut of inflammatory bowel disease (IBD) patients. However, the underlying mechanism of such changes has not been determined. In the current study, it was examined whether gut serotonin (5-hydroxytryptamine, 5-HT) can induce adherent-invasive E. coli colonization and increase TLR expression. In a co-culture system, commensal E. coli strain (BW25113, BW) adhered minimally to colon epithelial cells, but this was significantly enhanced by 5-HT to the level of a pathogenic strain (EDL933)...
February 17, 2017: Free Radical Biology & Medicine
https://www.readbyqxmd.com/read/28193461/gill-structural-integrity-changes-in-fish-deficient-or-excessive-in-dietary-isoleucine-towards-the-modulation-of-tight-junction-protein-inflammation-apoptosis-and-antioxidant-defense-via-nf-%C3%AE%C2%BAb-tor-and-nrf2-signaling-pathways
#4
Lin Feng, Lu Gan, Wei-Dan Jiang, Pei Wu, Yang Liu, Jun Jiang, Ling Tang, Sheng-Yao Kuang, Wu-Neng Tang, Yong-An Zhang, Xiao-Qiu Zhou
This study firstly aimed to test the impact of dietary isoleucine (Ile) on tight junction protein, inflammation, apoptosis, antioxidant defense and related signaling molecule gene expression in the gill of fish. Young grass carp (Ctenopharyngodon idella) (weighing 256.8 ± 3.5 g) were fed six diets containing graded levels of Ile, namely, 3.8, 6.6, 9.3, 12.5, 15.2 and 18.5 g/kg diet for 8 weeks. The results firstly revealed that Ile deficiency down-regulated the mRNA expressions of claudin-3, claudin-b, claudin-c, occludin and zonula occludens-1 (ZO-1) and up-regulated the mRNA expression of claudin-12, which led to the intercellular structure damage of fish gill...
February 11, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28191699/ndrg1-is-important-to-maintain-the-integrity-of-airway-epithelial-barrier-through-claudin-9-expression
#5
Yasuhiro Gon, Shuichiro Maruoka, Hiroyuki Kishi, Yutaka Kozu, Kuroda Kazumichi, Yasuyuki Nomura, Ikuko Takeshita, Takeshi Oshima, Shu Hashimoto
Impairment of epithelial barrier integrity caused by environmental triggers is associated with the pathogenesis of airway inflammation. Using human airway epithelial cells, we attempted to identify molecule(s) that promote airway epithelial barrier integrity. Microarray analyses were conducted using the Affimetrix human whole genome gene chip, and we identified the N-myc downstream-regulated gene 1 (NDRG1) gene, which was induced during the development of the epithelial cell barrier. Immunohistochemical analysis revealed strong NDRG1 expression in ciliated epithelial cells in nasal tissues sampled from patients with chronic rhinosinusitis (CRS), and the low expression of NDRG1 was observed in goblet cells or damaged epithelial cells...
February 13, 2017: Cell Biology International
https://www.readbyqxmd.com/read/28190892/slit2-robo4-signaling-potential-role-of-a-vegf-antagonist-pathway-to-regulate-luteal-permeability
#6
I Bekes, V Haunerdinger, R Sauter, I Holzheu, W Janni, A Wöckel, C Wulff
Introduction The corpus luteum (CL) is dependent on luteal vascular permeability, which is controlled by human chorionic gonadotropin (hCG) via vascular endothelial growth factor (VEGF). In this study we investigated the role of a potential VEGF antagonist pathway - Slit2/Robo4 - and its influence on endothelial cell adhesion. Materials and Methods Luteinized granulosa cells (LGCs) were stimulated with hCG in the absence or presence of a VEGF inhibitor. The expression of VEGF and Slit2 were measured. Human umbilical vein endothelial cells (HUVECs) were stimulated with Slit2 or VEGF, and gene expressions of cadherin 5 (CDH5) and claudin 5 (CLDN5) were measured...
January 2017: Geburtshilfe und Frauenheilkunde
https://www.readbyqxmd.com/read/28188749/loss-of-par-3-protein-expression-is-associated-with-invasion-lymph-node-metastasis-and-poor-survival-in-esophageal-squamous-cell-carcinoma
#7
Tomoko Kitaichi, Kohichiroh Yasui, Yasuyuki Gen, Osamu Dohi, Naoto Iwai, Akira Tomie, Nobuhisa Yamada, Kei Terasaki, Atsushi Umemura, Taichiro Nishikawa, Kanji Yamaguchi, Michihisa Moriguchi, Yoshio Sumida, Hironori Mitsuyoshi, Yuji Naito, Yoh Zen, Yoshito Itoh
Disrupted cell polarity is a feature of epithelial cancers. The partitioning defective 3 (PAR-3) protein, a key component of the PAR complex that regulates the polarization of cells, is involved in tight junction formation at epithelial cell-cell contacts. Our previous study detected a homozygous deletion of the PAR-3 gene in esophageal squamous cell carcinoma (ESCC) cell lines and frequent copy number loss of the PAR-3 gene in primary ESCC. Here, we aimed to investigate the clinicopathological relevance of altered expression of the PAR-3 protein in primary ESCC...
February 8, 2017: Human Pathology
https://www.readbyqxmd.com/read/28185956/long-non-coding-rna-neat1-regulates-permeability-of-the-blood-tumor-barrier-via-mir-181d-5p-mediated-expression-changes-in-zo-1-occludin-and-claudin-5
#8
Junqing Guo, Heng Cai, Jun Ma, Xiaobai Liu, Yunhui Liu, Jian Zheng, Zhongyou Que, Wei Gong, Yana Gao, Wei Tao, Yixue Xue
The blood-tumor barrier (BTB) constitutes an efficient organization of tight junctions that limits the delivery of chemotherapeutic drugs to brain tumor tissues and impacts the treatment of glioma. Long non-coding RNAs (lncRNAs) are non-protein coding RNAs regulating gene expression, some lncRNAs play a crucial role in BTB permeability. However, the function of lncRNAs in BTB permeability is still largely unclear. Here, we have identified lncRNA nuclear paraspeckle assembly transcript 1 (NEAT1), was remarkably up-regulated in glioma endothelial cells (GECs) obtained from an in vitro BTB model...
February 6, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28148288/metalloprotease-disintegrin-adam12-actively-promotes-the-stem-cell-like-phenotype-in-claudin-low-breast-cancer
#9
Sara Duhachek-Muggy, Yue Qi, Randi Wise, Linda Alyahya, Hui Li, Jacob Hodge, Anna Zolkiewska
BACKGROUND: ADAM12 is upregulated in human breast cancers and is a predictor of chemoresistance in estrogen receptor-negative tumors. ADAM12 is induced during epithelial-to-mesenchymal transition, a feature associated with claudin-low breast tumors, which are enriched in cancer stem cell (CSC) markers. It is currently unknown whether ADAM12 plays an active role in promoting the CSC phenotype in breast cancer cells. METHODS: ADAM12 expression was downregulated in representative claudin-low breast cancer cell lines, SUM159PT and Hs578T, using siRNA transfection or inducible shRNA expression...
February 1, 2017: Molecular Cancer
https://www.readbyqxmd.com/read/28118611/the-influence-of-neoadjuvant-chemotherapy-on-gastric-cancer-patients-postoperative-infectious-complications-what-is-the-negative-role-played-by-the-intestinal-barrier-dysfunction
#10
Zhiliang Wei, Bin Tan, Shougen Cao, Shanglong Liu, Xiaojie Tan, Zengwu Yao, Na Yin, Jiante Li, Dongfeng Zhang, Yanbing Zhou
Evidence has shown that neoadjuvant chemotherapy (NACT) is correlated with patients' overall postoperative complications. But investigations on relationship between NACT and postoperative infectious complications, which is closely linked to intestinal barrier damage, were scanty. Accordingly, 90 patients with advanced gastric cancer were included in this study. The differences in postoperative infectious complications were determined between NACT group in which patients received NACT before surgery and SURG group in which received surgical treatment immediately after diagnosis...
January 18, 2017: Oncotarget
https://www.readbyqxmd.com/read/28094766/matriptase-mediated-cleavage-of-epcam-destabilizes-claudins-and-dysregulates-intestinal-epithelial-homeostasis
#11
Chuan-Jin Wu, Xu Feng, Michael Lu, Sohshi Morimura, Mark C Udey
Congenital tufting enteropathy (CTE) is a severe autosomal recessive human diarrheal disorder with characteristic intestinal epithelial dysplasia. CTE can be caused by mutations in genes encoding EpCAM, a putative adhesion molecule, and HAI-2, a cell surface protease inhibitor. A similar phenotype occurs in mice whose intestinal epithelial cells (IECs) fail to express the tight junction-associated protein claudin-7. EpCAM stabilizes claudin-7 in IECs, and HAI-2 regulates the cell surface serine protease matriptase, a known modifier of intestinal epithelial physiology...
February 1, 2017: Journal of Clinical Investigation
https://www.readbyqxmd.com/read/28068922/functional-changes-in-mrna-expression-and-alternative-pre-mrna-splicing-associated-with-the-effects-of-nutrition-on-apoptosis-and-spermatogenesis-in-the-adult-testis
#12
Yongjuan Guan, Guanxiang Liang, Graeme B Martin, Le Luo Guan
BACKGROUND: The effects of nutrition on testis mass in the sexually mature male have long been known, however, the cellular and molecular processes of the testis response to nutrition was not fully understood. METHODS: We tested whether the defects in spermatogenesis and increases in germ cell apoptosis in the testis that are induced by under-nutrition are associated with changes in mRNA expression and pre-mRNA alternative splicing using groups of 8 male sheep fed for a 10% increase or 10% decrease in body mass over 65 days...
January 10, 2017: BMC Genomics
https://www.readbyqxmd.com/read/28018313/outer-membrane-vesicles-and-soluble-factors-released-by-probiotic-escherichia-coli-nissle-1917-and-commensal-ecor63-enhance-barrier-function-by-regulating-expression-of-tight-junction-proteins-in-intestinal-epithelial-cells
#13
Carina-Shianya Alvarez, Josefa Badia, Manel Bosch, Rosa Giménez, Laura Baldomà
The gastrointestinal epithelial layer forms a physical and biochemical barrier that maintains the segregation between host and intestinal microbiota. The integrity of this barrier is critical in maintaining homeostasis in the body and its dysfunction is linked to a variety of illnesses, especially inflammatory bowel disease. Gut microbes, and particularly probiotic bacteria, modulate the barrier integrity by reducing gut permeability and reinforcing tight junctions. Probiotic Escherichia coli Nissle 1917 (EcN) is a good colonizer of the human gut with proven therapeutic efficacy in the remission of ulcerative colitis in humans...
2016: Frontiers in Microbiology
https://www.readbyqxmd.com/read/27999452/microrna-regulation-of-endothelial-junction-proteins-and-clinical-consequence
#14
REVIEW
Yugang Zhuang, Hu Peng, Victoria Mastej, Weiguo Chen
Cellular junctions play a critical role in structural connection and signal communication between cells in various tissues. Although there are structural and functional varieties, cellular junctions include tight junctions, adherens junctions, focal adhesion junctions, and tissue specific junctions such as PECAM-1 junctions in endothelial cells (EC), desmosomes in epithelial cells, and hemidesmosomes in EC. Cellular junction dysfunction and deterioration are indicative of clinical diseases. MicroRNAs (miRNA) are ~20 nucleotide, noncoding RNAs that play an important role in posttranscriptional regulation for almost all genes...
2016: Mediators of Inflammation
https://www.readbyqxmd.com/read/27982694/cigarette-smoke-and-decreased-oxygen-tension-inhibit-pulmonary-claudin-6-expression
#15
Felix R Jimenez, Josh B Lewis, Samuel T Belgique, Dallin C Milner, Adam L Lewis, Todd M Dunaway, Kaleb M Egbert, Duane R Winden, Juan A Arroyo, Paul R Reynolds
PURPOSE: Chronic obstructive pulmonary disease is a condition involving perturbed barrier integrity coincident with both emphysema and inflammation of the airways, and smoking is considered a major risk factor. Claudins (Cldns) stabilize barriers and contribute to tight junctions by preventing paracellular transport of extracellular fluid constituents. METHODS: To determine Cldn6 was differentially influenced by tobacco smoke, Cldn6 was evaluated in cells and tissues by q-PCR, immunoblotting, and immunohistochemistry following exposure...
December 16, 2016: Experimental Lung Research
https://www.readbyqxmd.com/read/27942952/one-gene-two-paracellular-ion-channels-claudin-10-in-the-kidney
#16
REVIEW
Susanne Milatz, Tilman Breiderhoff
Claudins are tight junction membrane proteins and regulate the paracellular passage of ions and water. They can seal the paracellular cleft against solute passage but also form paracellular channels. They are tetraspan proteins with two extracellular segments. Claudin-10 exists in at least two functional isoforms, claudin-10a and claudin-10b, that differ in their first transmembrane segment and first extracellular segment. Both isoforms act as selective paracellular ion channels, either for anions (claudin-10a) or for cations (claudin-10b)...
January 2017: Pflügers Archiv: European Journal of Physiology
https://www.readbyqxmd.com/read/27915449/common-variants-in-cldn14-are-associated-with-differential-excretion-of-magnesium-over-calcium-in-urine
#17
REVIEW
Tanguy Corre, Eric Olinger, Sarah E Harris, Michela Traglia, Sheila Ulivi, Stefania Lenarduzzi, Hendrica Belge, Sonia Youhanna, Natsuko Tokonami, Olivier Bonny, Pascal Houillier, Ozren Polasek, Ian J Deary, John M Starr, Daniela Toniolo, Paolo Gasparini, Peter Vollenweider, Caroline Hayward, Murielle Bochud, Olivier Devuyst
The nature and importance of genetic factors regulating the differential handling of Ca(2+) and Mg(2+) by the renal tubule in the general population are poorly defined. We conducted a genome-wide meta-analysis of urinary magnesium-to-calcium ratio to identify associated common genetic variants. We included 9320 adults of European descent from four genetic isolates and three urban cohorts. Urinary magnesium and calcium concentrations were measured centrally in spot urine, and each study conducted linear regression analysis of urinary magnesium-to-calcium ratio on ~2...
January 2017: Pflügers Archiv: European Journal of Physiology
https://www.readbyqxmd.com/read/27904960/active-and-passive-involvement-of-claudins-in-the-pathophysiology-of-intestinal-inflammatory-diseases
#18
REVIEW
Christian Barmeyer, Michael Fromm, Jörg-Dieter Schulzke
Intestinal inflammatory diseases, four of which are discussed here, are associated with alterations of claudins. In ulcerative colitis, diarrhea and antigen entry into the mucosa occurs. Claudin-2 is upregulated but data on other claudins are still limited or vary (e.g., claudin-1 and -4). Apart from that, tight junction changes contribute to diarrhea via a leak flux mechanism, while protection against antigen entry disappears behind epithelial gross lesions (erosions) and apoptotic foci. Crohn's disease is additionally characterized by a claudin-5 and claudin-8 reduction which plays an active role in antigen uptake already before gross lesions appear...
January 2017: Pflügers Archiv: European Journal of Physiology
https://www.readbyqxmd.com/read/27895742/comparative-analysis-of-gene-expression-profiles-of-gastric-cardia-adenocarcinoma-and-gastric-non-cardia-adenocarcinoma
#19
Bin Song, Juan Du, Neng Deng, Ji-Chen Ren, Zhen-Bo Shu
In the present study, gene expression profiles were analyzed to identify the molecular mechanisms underlying gastric cardia adenocarcinoma (GCA) and gastric non-cardia adenocarcinoma (GNCA). A gene expression dataset (accession number GSE29272) was downloaded from Gene Expression Omnibus, and consisted of 62 GCA samples and 62 normal controls, as well as 72 GNCA samples and 72 normal controls. The two groups of differentially-expressed genes (DEGs) were compared to obtain common and unique DEGs. A differential analysis was performed using the Linear Models for Microarray Data package in R...
November 2016: Oncology Letters
https://www.readbyqxmd.com/read/27888130/protective-role-of-phenylalanine-on-the-ros-induced-oxidative-damage-apoptosis-and-tight-junction-damage-via-nrf2-tor-and-nf-%C3%AE%C2%BAb-signalling-molecules-in-the-gill-of-fish
#20
Lin Feng, Wen Li, Yang Liu, Wei-Dan Jiang, Sheng-Yao Kuang, Pei Wu, Jun Jiang, Ling Tang, Wu-Neng Tang, Yong-An Zhang, Xiao-Qiu Zhou
This study explored the possible preventive effects of dietary phenylalanine (Phe) on antioxidant responses, apoptosis and tight junction protein transcription in the gills of young grass carp (Ctenopharyngodon idella). Fish were fed six different experimental diets containing graded levels of Phe (3.4-16.8 g kg(-1)) for 8 weeks. The results showed that Phe deficiency induced protein oxidation and lipid peroxidation by decreasing the glutathione content and the activities and mRNA levels of Cu/Zn superoxide dismutase (SOD1), catalase (CAT), glutathione peroxidase (GPx), glutathione reductase (GR) and glutathione-S-transferase (GST) in fish gill (P < 0...
November 23, 2016: Fish & Shellfish Immunology
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