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claudin gene regulation

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https://www.readbyqxmd.com/read/29745557/-effects-of-silencing-snail1-gene-on-the-expression-of-tight-junction-proteins-and-the-migration-ability-of-hep-2-cells
#1
Shuangfeng Liu, Yang Shen, Tang Feng, Xiaoheng Liu
To investigate the effects of Snail1 gene silence on the expression of tight junction proteins and the migration ability of Hep-2 cells, Hep-2 cells were transfected with plasmids which is containing the shRNA of Snail1 gene, and cultured till the cells could be passaged stably (named Sh-snail1 cells). The expression of tight junction proteins (ZO-1, Occludin, Claudin-5) were detected by Western blot. The migration ability of Sh-snail1 cells was investigated by wound healing assay, and the protein expression of members of RhoGTPase family (RhoA, Cdc42) was detected by Western blot, which is closely related to the migration ability...
August 1, 2017: Sheng Wu Yi Xue Gong Cheng Xue za Zhi, Journal of Biomedical Engineering, Shengwu Yixue Gongchengxue Zazhi
https://www.readbyqxmd.com/read/29731129/early-onset-pediatric-atopic-dermatitis-is-characterized-by-t-h-2-t-h-17-t-h-22-centered-inflammation-and-lipid-alterations
#2
Patrick M Brunner, Ariel Israel, Ning Zhang, Alexandra Leonard, Huei-Chi Wen, Thy Huynh, Gary Tran, Sarah Lyon, Giselle Rodriguez, Supriya Immaneni, Annette Wagner, Xiuzhong Zheng, Yeriel D Estrada, Hui Xu, James G Krueger, Amy S Paller, Emma Guttman-Yassky
BACKGROUND: Although atopic dermatitis (AD) often starts in early childhood, detailed tissue profiling of early-onset AD in children is lacking, hindering therapeutic development for this patient population with a particularly high unmet need for better treatments. OBJECTIVE: We sought to globally profile the skin of infants with AD compared with that of adults with AD and healthy control subjects. METHODS: We performed microarray, RT-PCR, and fluorescence microscopy studies in infants and young children (<5 years old) with early-onset AD (<6 months disease duration) compared with age-matched control subjects and adults with longstanding AD...
April 19, 2018: Journal of Allergy and Clinical Immunology
https://www.readbyqxmd.com/read/29722942/-role-of-tgf-%C3%AE-1-in-sertoli-cells-and-tight-junction
#3
Jiang-Tao Lou, Ren-Xiong Wei, Lang-Lang Yu, Jian-Wei Chen, Yun Cui
Objective: To explore the role of TGF-β1 in the proliferation and apoptosis of Sertoli cells and its effect on the expressions of tight junction-related proteins and genes in rats. METHODS: Rat Sertoli cells were isolated in vitro, primarily cultured, and divided into groups A (blank control), B (TGF-β1 receptor blocker), C (TGF-β1), and D (TGF-β1 + receptor blocker). The proliferation and apoptosis of the cells were detected by CCK-8 and flow cytometry, respectively...
June 2017: Zhonghua Nan Ke Xue, National Journal of Andrology
https://www.readbyqxmd.com/read/29705796/clostridium-tyrobutyricum-protect-intestinal-barrier-function-from-lps-induced-apoptosis-via-p38-jnk-signaling-pathway-in-ipec-j2-cells
#4
Zhiping Xiao, Lujie Liu, Wenjing Tao, Xun Pei, Geng Wang, Minqi Wang
BACKGROUND/AIMS: The intestinal mucosa forms a physical and metabolic barrier against the diffusion of pathogens, toxins, and allergens from the lumen into the circulatory system. Early weaning, a critical phase in swine production, can compromise intestinal barrier function through mucosal damage and alteration of tight junction integrity Maintenance of intestinal barrier function plays a pivotal role in optimum gastrointestinal health. In this study, we investigated the effects of Clostridium tyrobutyricum (C...
April 25, 2018: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/29696536/transgenic-up-regulation-of-claudin-6-decreases-fine-diesel-particulate-matter-dpm-induced-pulmonary-inflammation
#5
Joshua B Lewis, Jared S Bodine, Jason R Gassman, Samuel Arce Muñoz, Dallin C Milner, Todd M Dunaway, Kaleb M Egbert, Troy D Monson, Dallin S Broberg, Juan A Arroyo, Paul R Reynolds
Claudin-6 (Cldn6) is a tetraspanin transmembrane protein that contributes to tight junctional complexes and has been implicated in the maintenance of lung epithelial barriers. In the present study, we tested the hypothesis that genetic up-regulation of Cldn-6 influences inflammation in mice exposed to short-term environmental diesel particulate matter (DPM). Mice were subjected to ten exposures of nebulized DPM (PM2.5) over a period of 20 days via a nose-only inhalation system (Scireq, Montreal, Canada). Using real-time RT-PCR, we discovered that the Cldn6 gene was up-regulated in control mice exposed to DPM and in lung-specific transgenic mice that up-regulate Cldn-6 (Cldn-6 TG)...
April 25, 2018: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/29686978/novel-aspects-of-renal-magnesium-homeostasis
#6
REVIEW
Paula Giménez-Mascarell, Carlotta Else Schirrmacher, Luis Alfonso Martínez-Cruz, Dominik Müller
Magnesium (Mg2+ ) is indispensable for several vital functions, such as neurotransmission, cardiac conductance, blood glucose, blood pressure regulation, and proper function of more than 300 enzymes. Thus, Mg2+ homeostasis is subject to tight regulation. Besides the fast and immediate regulation of plasma Mg2+ , a major part of Mg2+ homeostasis is realized by a concerted action of epithelial molecular structures that tightly control intestinal uptake and renal absorption. This mechanism is provided by a combination of para- and transcellular pathways...
2018: Frontiers in Pediatrics
https://www.readbyqxmd.com/read/29650692/microrna-mediated-therapy-modulating-blood-brain-barrier-disruption-improves-vascular-cognitive-impairment
#7
Kensuke Toyama, Joshua M Spin, Alicia C Deng, Ting-Ting Huang, Ke Wei, Markus U Wagenhäuser, Takuya Yoshino, Huy Nguyen, Joscha Mulorz, Soumajit Kundu, Uwe Raaz, Matti Adam, Isabel N Schellinger, Ann Jagger, Philip S Tsao
OBJECTIVE: There are currently no effective treatments for the prevention of dementia associated with vascular cognitive impairment. MicroRNAs regulate gene expression at the post-transcriptional level and play key roles in vascular disorders. TNFα (tumor necrosis factor-α) regulates blood-brain barrier breakdown through modification of cerebral tight junctions. Here, we sought key TNFα-responsive microRNAs that might influence blood-brain barrier breakdown via cerebral tight junction disruption in vascular cognitive impairment...
April 12, 2018: Arteriosclerosis, Thrombosis, and Vascular Biology
https://www.readbyqxmd.com/read/29582532/extracellular-htra-serine-proteases-an-emerging-new-strategy-in-bacterial-pathogenesis
#8
REVIEW
Steffen Backert, Sabine Bernegger, Joanna Skórko-Glonek, Silja Wessler
The HtrA family of chaperones and serine proteases is important for regulating stress responses and controlling protein quality in the periplasm of bacteria. HtrA is also associated with infectious diseases since inactivation of htrA genes results in significantly reduced virulence properties by various bacterial pathogens. These virulence features of HtrA can be attributed to reduced fitness of the bacteria, higher susceptibility to environmental stress and/or diminished secretion of virulence factors. In some Gram-negative and Gram-positive pathogens, HtrA itself can be exposed to the extracellular environment promoting bacterial colonization and invasion of host tissues...
March 26, 2018: Cellular Microbiology
https://www.readbyqxmd.com/read/29562342/diquat-induced-oxidative-stress-increases-intestinal-permeability-impairs-mitochondrial-function-and-triggers-mitophagy-in-piglets
#9
Shuting Cao, Huan Wu, ChunChun Wang, Qianhui Zhang, Lefei Jiao, Fanghui Lin, Caihong Hu
In the present study, we investigated the influence of diquat-induced oxidative stress on intestinal barrier, mitochondrial function and the level of mitophagy in piglets. Twelve male Duroc×Landrace×Yorkshire 35-d-old pigs (weaned at 21d of age), with an average body of 9.6 kg, were allotted to two treatments of six piglets each including the challenged group and the control group. The challenged pigs were injected with 100 mg/kg bodyweight diquat and control pigs injected with 0.9% (w/v) NaCl solution. The results showed that diquat injection decreased average daily feed intake and average daily gain...
March 17, 2018: Journal of Animal Science
https://www.readbyqxmd.com/read/29541196/assessment-of-cxc-ligand-12-mediated-calcium-signalling-and-its-regulators-in-basal-like-breast-cancer-cells
#10
S Y N Jamaludin, I Azimi, F M Davis, A A Peters, T J Gonda, E W Thompson, S J Roberts-Thomson, G R Monteith
CXC ligand (L)12 is a chemokine implicated in the migration, invasion and metastasis of cancer cells via interaction with its receptors CXC chemokine receptor (CXCR)4 and CXCR7. In the present study, CXCL12-mediated Ca2+ signalling was compared with two basal-like breast cancer cell lines, MDA-MB-231 and MDA-MB-468, which demonstrate distinct metastatic potential. CXCL12 treatment induced Ca2+ responses in the more metastatic MDA-MB-231 cells but not in the less metastatic MDA-MB-468 cells. Assessment of mRNA levels of CXCL12 receptors and their potential modulators in both cell lines revealed that CXCR4 and CXCR7 levels were increased in MDA-MB-231 cells compared with MDA-MB-468 cells...
April 2018: Oncology Letters
https://www.readbyqxmd.com/read/29538454/infection-with-hepatitis-c-virus-depends-on-tacstd2-a-regulator-of-claudin-1-and-occludin-highly-downregulated-in-hepatocellular-carcinoma
#11
Vandana Sekhar, Teresa Pollicino, Giacomo Diaz, Ronald E Engle, Farah Alayli, Marta Melis, Juraj Kabat, Ashley Tice, Anna Pomerenke, Nihal Altan-Bonnet, Fausto Zamboni, Paolo Lusso, Suzanne U Emerson, Patrizia Farci
Entry of hepatitis C virus (HCV) into hepatocytes is a complex process that involves numerous cellular factors, including the scavenger receptor class B type 1 (SR-B1), the tetraspanin CD81, and the tight junction (TJ) proteins claudin-1 (CLDN1) and occludin (OCLN). Despite expression of all known HCV-entry factors, in vitro models based on hepatoma cell lines do not fully reproduce the in vivo susceptibility of liver cells to primary HCV isolates, implying the existence of additional host factors which are critical for HCV entry and/or replication...
March 14, 2018: PLoS Pathogens
https://www.readbyqxmd.com/read/29515533/-vibrio-tapetis-displays-an-original-type-iv-secretion-system-in-strains-pathogenic-for-bivalve-molluscs
#12
Graciela M Dias, Adeline Bidault, Patrick Le Chevalier, Gwenaëlle Choquet, Clio Der Sarkissian, Ludovic Orlando, Claudine Medigue, Valerie Barbe, Sophie Mangenot, Cristiane C Thompson, Fabiano L Thompson, Annick Jacq, Vianney Pichereau, Christine Paillard
The Brown Ring Disease (BRD) caused high mortality rates since 1986 in the Manila clam Venerupis philippinarum introduced and cultured in Western Europe from the 1970s. The causative agent of BRD is a Gram-Negative bacterium, Vibrio tapetis , which is also pathogenic to fish. Here we report the first assembly of the complete genome of V. tapetis CECT4600T , together with the genome sequences of 16 additional strains isolated across a broad host and geographic range. Our extensive genome dataset allowed us to describe the pathogen pan- and core genomes and to identify putative virulence factors...
2018: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29510139/elk3-gata3-axis-modulates-mda-mb-231-metastasis-by-regulating-cell-cell-adhesion-related-genes
#13
Kwang-Soo Kim, Jiewan Kim, Nuri Oh, Mi-Young Kim, Kyung-Soon Park
GATA3 is a master regulator that drives mammary epithelial cell differentiation, and the suppression of GATA3 expression is associated with the development of aggressive breast cancer. However, the mechanism through which GATA3 loss drives cancer development is poorly understood. Previously, we reported that ELK3 suppression in MDA-MB-231 (ELK3 KD) resulted in the reprogramming of these cells from a basal to luminal subtype, which was associated with the induction of GATA3 expression, and that the ELK3-GATA3 axis orchestrated the metastatic characteristics of MDA-MB-231...
April 6, 2018: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29508680/medium-chain-tag-improve-intestinal-integrity-by-suppressing-toll-like-receptor-4-nucleotide-binding-oligomerisation-domain-proteins-and-necroptosis-signalling-in-weanling-piglets-challenged-with-lipopolysaccharide
#14
Xiao Xu, Shaokui Chen, Haibo Wang, Zhixiao Tu, Shuhui Wang, Xiuying Wang, Huiling Zhu, Chunwei Wang, Jiadong Zhu, Yulan Liu
This study was conducted to evaluate whether medium-chain TAG (MCT) could alleviate Escherichia coli lipopolysaccharide (LPS)-induced intestinal injury by regulating intestinal epithelial inflammatory response, as well as necroptosis. A total of twenty-four weanling piglets were randomly allotted to one of four treatments in a 2×2 factorial arrangement including diet type (5 % maize oil v. 4 % MCT+1 % maize oil) and immune stress (saline v. E. coli LPS). The piglets were fed diets containing maize oil or MCT for 21 d...
May 2018: British Journal of Nutrition
https://www.readbyqxmd.com/read/29482498/high-level-cytoplasmic-claudin-3-expression-is-an-independent-predictor-of-poor-survival-in-triple-negative-breast-cancer
#15
Anniina Jääskeläinen, Ylermi Soini, Arja Jukkola-Vuorinen, Päivi Auvinen, Kirsi-Maria Haapasaari, Peeter Karihtala
BACKGROUND: The subtype of claudin-low breast cancer can be reliably determined only by gene-expression profiling. Attempts have been made to develop immunohistochemical surrogates, which nearly always focus on membranous claudin expression. METHODS: We assessed the immunohistochemical expression of both membranous and cytoplasmic claudins 3, 4 and 7 in a series of 197 non-metastatic breast cancers, enriched with triple-negative breast cancers (TNBCs; 60%). The expression of epithelial-to-mesenchymal transition-regulating transcription factors Sip1, Zeb1 and vimentin had previously been determined in the same material...
February 27, 2018: BMC Cancer
https://www.readbyqxmd.com/read/29467620/mir-429-regulated-by-endothelial-monocyte-activating-polypeptide-ii-emap-ii-influences-blood-tumor-barrier-permeability-by-inhibiting-the-expressions-of-zo-1-occludin-and-claudin-5
#16
Liangyu Chen, Yixue Xue, Jian Zheng, Xiaobai Liu, Jing Liu, Jiajia Chen, Zhen Li, Zhuo Xi, Hao Teng, Ping Wang, Libo Liu, Yunhui Liu
The blood-tumor barrier (BTB) hinders delivery of chemotherapeutic drugs to tumors in the brain; previous studies have shown that the BTB can be selectively opened by endothelial monocyte activating polypeptide-II (EMAP-II), but the specific mechanism involved remains elusive. In this study, we found that microRNA-429 (miR-429) expression in glioma vascular endothelial cells (GECs) was far lower than in human brain microvascular endothelial cells (ECs). miR-429 had lower expression in GECs and glioma tissues compared to ECs or normal tissues of the brain...
2018: Frontiers in Molecular Neuroscience
https://www.readbyqxmd.com/read/29440295/microrna-203-inhibits-long-noncoding-rna-hotair-and-regulates-tumorigenesis-through-epithelial-to-mesenchymal-transition-pathway-in-renal-cell-carcinoma
#17
Pritha Dasgupta, Priyanka Kulkarni, Shahana Majid, Varahram Shahryari, Yutaka Hashimoto, Nadeem S Bhat, Marisa Shiina, Guoren Deng, Sharanjot Saini, Z Laura Tabatabai, Soichiro Yamamura, Yuichiro Tanaka, Rajvir Dahiya
This study aims to investigate the role of miR-203-HOTAIR interaction in the suppression of renal cell carcinoma (RCC). We employed series of in vitro assays such as proliferation, invasion, migration, and colony formation along with in vivo tumor xenograft model. Profiling of miR-203 and HOTAIR expression revealed that miR-203 was significantly underexpressed, whereas HOTAIR was overexpressed in RCC cell lines and clinical specimens compared with normal cell line and tissue. Both miR-203 and HOTAIR expression significantly distinguished malignant from normal tissues and significantly correlated with clinicopathologic characteristics of patients...
May 2018: Molecular Cancer Therapeutics
https://www.readbyqxmd.com/read/29435034/gadd45%C3%AE-silencing-impaired-viability-and-metastatic-phenotypes-in-cholangiocarcinoma-cells-by-modulating-the-emt-pathway
#18
Kyaw Zwar Myint, Pornparn Kongpracha, Panthip Rattanasinganchan, Kawin Leelawat, Penpak Moolthiya, Kittipong Chaiyabutr, Rutaiwan Tohtong
Growth arrest and DNA damage-inducible-β (Gadd45β) is a stress-response protein involved in a number of processes, including cell cycle control, DNA repair, survival and death control, and stress signaling, depending on its interactions. Gadd45β expression is dysregulated in numerous types of cancer, functioning as either a tumor promoter or a tumor suppressor. However, the functions of Gadd45β in cholangiocarcinoma (CCA), particularly in metastasis, has not been studied. The immunohistochemical analysis of Gadd45β expression revealed that 75% of histological specimens from patients with CCA expressed high levels of Gadd45β, and that high Gadd45β expression was associated with metastasis...
March 2018: Oncology Letters
https://www.readbyqxmd.com/read/29424664/chemotaxis-like-pathway-of-azorhizobium-caulinodans-controls-flagella-driven-motility-which-regulates-biofilms-formation-exopolysaccharide-biosynthesis-and-competitive-nodulation
#19
Zhihong Xie, Wei Liu, Yu Sun, Rimin Shen, Xiaoxiao Dang, Xiaolin Liu, Fu Sui, Yan Li, Zhenpeng Zhang, Gladys Alexandre, Claudine Elmerich
The genome of the Azorhizobium caulinodans ORS571 contains a unique chemotaxis gene cluster (che) including 5 chemotaxis genes: cheAWY1BR. Analysis of the role of the chemotaxis cluster of A. caulinodans, using deletion mutant strains revealed that CheA or Che signaling pathway controls chemotaxis behavior and flagella-driven motility, and plays important roles in biofilms formation and production of extracellular polysaccharides. Furthermore, the deletion mutants (ΔcheA and ΔcheA-R) were defective in competitive adsorption and colonization on the root surface of host plants...
February 9, 2018: Molecular Plant-microbe Interactions: MPMI
https://www.readbyqxmd.com/read/29402269/expression-of-a-recombinant-hybrid-antimicrobial-peptide-magainin-ii-cecropin-b-in-the-mycelium-of-the-medicinal-fungus-cordyceps-militaris-and-its-validation-in-mice
#20
Min Zhang, Yuanlong Shan, Hongtao Gao, Bin Wang, Xin Liu, Yuanyuan Dong, Xiuming Liu, Na Yao, Yonggang Zhou, Xiaowei Li, Haiyan Li
BACKGROUND: Antibiotic residues can cause antibiotic resistance in livestock and their food safety-related issues have increased the consumer demand for products lacking these residues. Hence, developing safe and effective antibiotic alternatives is important to the animal feed industry. With their strong antibacterial actions, antimicrobial peptides have potential as antibiotic alternatives. RESULTS: We investigated the antibacterial and immunomodulatory activities and the mechanisms of action of an antimicrobial peptide...
February 5, 2018: Microbial Cell Factories
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