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https://www.readbyqxmd.com/read/29474232/protein-content-of-cervicovaginal-fluid-is-altered-during-bacterial-vaginosis
#1
Carolina Sanitá Tafner Ferreira, Márcia Guimarães da Silva, Letícia Gomes de Pontes, Lucilene Delazari Dos Santos, Camila Marconi
OBJECTIVES: The aim of the study was to compare, using a proteomic approach, cervicovaginal fluid (CVF) proteins of women with bacterial vaginosis (BV) with those presenting normal microbiota. MATERIALS AND METHODS: A total of 309 reproductive-aged women were cross-sectionally enrolled. Participants were tested for vaginal candidosis, Trichomonas vaginalis, Chlamydia trachomatis, and Neisseria gonorrhoeae and excluded if positive. Vaginal microbiota was classified microscopically according to Nugent criteria in normal, intermediate, and BV...
February 23, 2018: Journal of Lower Genital Tract Disease
https://www.readbyqxmd.com/read/29471763/risk-factors-associated-with-sexually-transmitted-infections-among-women-under-community-supervision-in-new-york-city
#2
Alissa Davis, Dawn Goddard-Eckrich, Anindita Dasgupta, Nabila El-Bassel
The number of women under community supervision in the United States has increased, and this population has a high risk for sexually transmitted infections (STIs). We examined STI prevalence and multiple risk factors among drug-involved women under community supervision in New York City. Data were from a randomized controlled trial testing the efficacy of a behavioral HIV/STI intervention (Women on the Road to Health [WORTH]) among drug-involved women in the community corrections system in New York City from 2009 to 2012...
January 1, 2018: International Journal of STD & AIDS
https://www.readbyqxmd.com/read/29465686/prevalence-and-detection-of-trichomonas-vaginalis-in-human-immunodeficiency-virus-infected-pregnant-women
#3
Collin M Price, Remco P H Peters, Janré Steyn, Maanda Mudau, Dawie Olivier, Lindsey De Vos, Erika Morikawa, Marleen M Kock, Andrew Medina-Marino, Jeffrey D Klausner
BACKGROUND: Trichomonas vaginalis is a STI associated with increased transmission of HIV and significant adverse birth outcomes; culture and PCR are commonly used in diagnosis. METHODS: Consenting HIV-infected pregnant women were recruited from clinics in South Africa and screened for T. vaginalis using PCR. PCR-positive women provided an additional sample for culture. We compared T. vaginalis detection between PCR and culture, and investigated how PCR cycle threshold (Ct) values differ among culture results...
November 7, 2017: Sexually Transmitted Diseases
https://www.readbyqxmd.com/read/29465684/screening-for-trichomonas-vaginalis-in-a-large-high-risk-population-prevalence-among-males-and-females-determined-by-nucleic-acid-amplification-testing
#4
J Schwebke, A Merriweather, S Massingale, M Scisney, C Hill, D Getman
Men and women attending family planning and sexually transmitted disease clinics for STI screening in 2012-2013 were tested for Trichomonas vaginalis (TV) using a sensitive nucleic acid amplification test. TV prevalence in urogenital samples from 77,740 women was 11.3%, 6.1% in 12,604 men, and increased with age in both genders.This is an open-access article distributed under the terms of the Creative Commons Attribution-Non Commercial-No Derivatives License 4.0 (CCBY-NC-ND), where it is permissible to download and share the work provided it is properly cited...
November 14, 2017: Sexually Transmitted Diseases
https://www.readbyqxmd.com/read/29465674/the-etiology-of-vaginal-discharge-syndrome-in-zimbabwe-results-from-the-zimbabwe-sti-etiology-study
#5
Z Mike Chirenje, Nicholas Dhibi, H Hunter Handsfield, Elizabeth Gonese, Beth Tippett Barr, Lovemore Gwanzura, Ahmed S Latif, Dumisili Venessa Maseko, Ranmini S Kularatne, Mufuta Tshimanga, Peter H Kilmarx, Anna Machiha, Owen Mugurungi, Cornelis A Rietmeijer
INTRODUCTION: Symptomatic vaginal discharge is a common gynecological condition managed syndromically in most developing countries. In Zimbabwe, women presenting with symptomatic vaginal discharge are treated with empirical regimens that commonly cover both sexually transmitted infections (STI) and reproductive tract infections, typically including a combination of an intramuscular injection of kanamycin, and oral doxycycline and metronidazole regimens. This study was conducted to determine the current etiology of symptomatic vaginal discharge and assess adequacy of current syndromic management guidelines...
November 29, 2017: Sexually Transmitted Diseases
https://www.readbyqxmd.com/read/29465667/prospective-evaluation-of-cervico-vaginal-self-and-cervical-physician-collection-for-the-detection-of-chlamydia-trachomatis-neisseria-gonorrhoeae-trichomonas-vaginalis-and-mycoplasma-genitalium-infections
#6
Alexandre Lockhart, Matt Psioda, Jie Ting, Sara Campbell, Nelly Mugo, Jessie Kwatampora, Michael Chitwa, Joshua Kimani, Anne Gakure, Jennifer S Smith
BACKGROUND: To examine the agreement between sexually transmitted infection (STI) screening using self-collected specimens and physician-collected specimens, and to investigate the acceptability of self-collection for screening in an 18-month study of female sex-workers (FSW) in a high-risk, low-resource setting. METHODS: A total of 350 FSW in Nairobi, Kenya participated in a prospective study from 2009-2011. Women self-collected a cervico-vaginal specimen. Next, a physician conducted a pelvic examination to obtain a cervical specimen...
January 2, 2018: Sexually Transmitted Diseases
https://www.readbyqxmd.com/read/29444981/protein-palmitoylation-plays-an-important-role-in-trichomonas-vaginalis-adherence
#7
Yesica R Nievas, Ajay A Vashisht, Maria M Corvi, Sebastian Metz, Patricia J Johnson, James A Wohlschlegel, Natalia de Miguel
The flagellated protozoan parasite Trichomonas vaginalis is the etiologic agent of trichomoniasis, the most common non-viral sexually transmitted infection worldwide. As an obligate extracellular pathogen, adherence to epithelial cells is critical for parasite survival within the human host and a better understanding of this process is a prerequisite for the development of therapies to combat infection. In this sense, recent work has shown S-acylation as a key modification that regulates pathogenesis in different protozoan parasites...
February 14, 2018: Molecular & Cellular Proteomics: MCP
https://www.readbyqxmd.com/read/29444462/photodynamic-therapy-as-a-new-approach-to-trichomonas-vaginalis-inactivation
#8
Thaisa Helena Silva Fonseca, Marina Alacoque, Fabrício Marcus Silva Oliveira, Betânia Maria Soares, Henrique Vitor Leite, Marcelo Vidigal Caliari, Maria Aparecida Gomes, Haendel Busatti
The emergence of nitroimidazole resistant isolates has been an aggravating factor in the treatment of trichomoniasis, the most common non-viral sexually transmitted disease in the world. This particularity highlights the importance of new technologies that are safe, effective, and have minor side effects or resistance. Hence, we evaluated the effectiveness of photodynamic therapy on the inactivation of Trichomonas vaginalis in vitro. We used methylene blue as a photosensitizing substance, and a light-emitting diode (LED) for irradiation of metronidazole sensitive and resistant strains...
February 11, 2018: Photodiagnosis and Photodynamic Therapy
https://www.readbyqxmd.com/read/29413946/trichomonas-vaginalis-cathepsin-d-like-aspartic-proteinase-tv-catd-is-positively-regulated-by-glucose-and-degrades-human-hemoglobin
#9
Maria Inocente Mancilla-Olea, Jaime Ortega-López, Elisa E Figueroa-Angulo, Leticia Avila-González, Rosa Elena Cárdenas-Guerra, Jesús F T Miranda-Ozuna, Arturo González-Robles, Mar Saraí Hernández-García, Lizbeth Sánchez-Ayala, Rossana Arroyo
Trichomonas vaginalis genome encodes ∼440 proteases, six of which are aspartic proteases (APs). However, only one belongs to a clan AA (EC 3.4.23.5), family A1 (pepsin A), cathepsin D-like protease. This AP is encoded by an 1113-bp gene (tv-catd), which translates into a 370-aa residues zymogen of 40.7-kDa and a theoretical pI of 4.6, generating a ∼35 kDa active enzyme after maturation (Tv-CatD). The goal of this study was to identify and analyze the effect of glucose on the expression of Tv-CatD at the transcript and protein levels, subcellular localization, and proteolytic activity...
January 29, 2018: International Journal of Biochemistry & Cell Biology
https://www.readbyqxmd.com/read/29409759/prevalence-of-chlamydia-trachomatis-neisseria-gonorrhoeae-and-trichomonas-vaginalis-infection-in-chilean-adolescents-and-young-adults
#10
Andrea Huneeus, Andrea Schilling, Mario I Fernandez
STUDY OBJECTIVE: To determine the prevalence of Chlamydia trachomatis, Neisseria gonorrhoeae, and Trichomonas vaginalis infection in sexually active Chilean adolescents and young adults. A comparative analysis was performed between genders in order to identify demographic, clinical, and sexual behavior characteristics to predict the occurrence of C.trachomatis. STUDY DESIGN: Analytical observational study SETTING: Santiago, Chile. PARTICIPANTS: 286 sexually active volunteers aged ≤24 (171 females and 115 males); 82% of them were classified as having high socioeconomic status...
January 31, 2018: Journal of Pediatric and Adolescent Gynecology
https://www.readbyqxmd.com/read/29408891/neutrophils-kill-the-parasite-trichomonas-vaginalis-using-trogocytosis
#11
Frances Mercer, Shek Hang Ng, Taylor M Brown, Grace Boatman, Patricia J Johnson
T. vaginalis, a human-infective parasite, causes the most common nonviral sexually transmitted infection (STI) worldwide and contributes to adverse inflammatory disorders. The immune response to T. vaginalis is poorly understood. Neutrophils (polymorphonuclear cells [PMNs]) are the major immune cell present at the T. vaginalis-host interface and are thought to clear T. vaginalis. However, the mechanism of PMN clearance of T. vaginalis has not been characterized. We demonstrate that human PMNs rapidly kill T...
February 6, 2018: PLoS Biology
https://www.readbyqxmd.com/read/29406595/tritrichomonas-foetus-and-mycoplasma-felis-coinfection-in-the-upper-respiratory-tract-of-a-cat-with-chronic-purulent-discharge
#12
Luca Pazzini, Linda Mugnaini, Francesca Mancianti, Lorenzo Ressel
A 5-year-old indoor male neutered Siamese cat was presented with clinical signs of sneezing and chronic bilateral purulent nasal discharge. Multiple nasal cavity swabs were submitted for bacterial cultures, Mycoplasma felis-DNA qPCR, and cytology. M felisqPCR was positive and cytomorphologic diagnosis was severe, acute, purulent, rhinitis with intralesional protozoal microorganisms consistent with a Trichomonas spp. Nested PCR (nPCR) confirmed the diagnosis of Tritrichomonas foetus. Systemic therapy with doxycycline for M felis and metronidazole for T foetus was started with remission of clinical signs within 2 weeks; however, symptoms relapsed shortly after therapy was discontinued...
February 6, 2018: Veterinary Clinical Pathology
https://www.readbyqxmd.com/read/29396352/risk-of-high-risk-human-papillomavirus-infection-and-cervical-precancerous-lesions-with-past-or-current-trichomonas-infection-a-pooled-analysis-of-25-054-women-in-rural-china
#13
Rui-Mei Feng, Margaret Z Wang, Jennifer S Smith, Li Dong, Feng Chen, Qin-Jing Pan, Xun Zhang, You-Lin Qiao, Fang-Hui Zhao
BACKGROUND: Trichomonas vaginitis (TV) infection has obviously been implicated in gynecological morbidity but still unclear in cervical lesions. OBJECTIVE: To evaluate the risk of hr-HPV infection and cervical intraepithelial neoplasia grade 2 or worse (CIN2 + ) by TV infection. STUDY DESIGN: The pooled study was conducted among 12 population-based, cervical cancer screening studies throughout China (N = 24,054). HPV was detected by Hybrid Capture®2 (HC2) test...
December 30, 2017: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/29393008/intravenous-metronidazole-liquid-tinidazole-and-intra-vaginal-boric-acid-to-cure-trichomonas-in-a-patient-with-gastric-bypass-surgery
#14
Saira Butt, Amir Tirmizi
This study presents a case report of a female patient with symptomatic refractory Trichomonas vaginalis infection who was not able to clear her infection with high-dose oral metronidazole, oral tinidazole, intra-vaginal zinc sulfate, intra-vaginal metronidazole, intra-vaginal tinidazole, and intra-vaginal boric acid. She was unable to tolerate intra-vaginal paromomycin. A combination of intravenous metronidazole, oral tinidazole liquid suspension, and intra-vaginal boric acid for 14 days subsequently achieved a complete symptomatic and laboratory cure...
January 1, 2018: International Journal of STD & AIDS
https://www.readbyqxmd.com/read/29391293/a-case-of-non-treated-trichomonas-vaginalis-infection-and-severe-preterm-labour-with-positive-obstetrical-outcome
#15
E Salakos, D Korb, C Morin, O Sibony
Trichomonas vaginalis is a very common, sexually transmitted, infection that may sometimes be asymptomatic or cause vaginitis and urethritis. Recently, it has been associated with adverse obstetric outcomes such as preterm delivery, low birth weight and premature rupture of membranes. Trichomonas vaginalis can be vertically transmitted at birth. It has been found in pharynx and low respiratory tract of neonates with respiratory disease. It has also been involved in some cases of intellectual disability. The recommended treatment is a 2g metronidazole oral single dose, even for asymptomatic patients...
January 29, 2018: Journal of Gynecology Obstetrics and Human Reproduction
https://www.readbyqxmd.com/read/29390009/chemical-composition-immunostimulatory-cytotoxic-and-antiparasitic-activities-of-the-essential-oil-from-brazilian-red-propolis
#16
Ângela Sena-Lopes, Francisco Silvestre Brilhante Bezerra, Raquel Nascimento das Neves, Rodrigo Barros de Pinho, Mara Thais de Oliveira Silva, Lucielli Savegnago, Tiago Collares, Fabiana Seixas, Karine Begnini, João Antonio Pêgas Henriques, Mariana Roesch Ely, Luciane C Rufatto, Sidnei Moura, Thiago Barcellos, Francine Padilha, Odir Dellagostin, Sibele Borsuk
Most studies of Brazilian red propolis have explored the composition and biological properties of its ethanolic extracts. In this work, we chemically extracted and characterized the essential oil of Brazilian red propolis (EOP) and assessed its adjuvant, antiparasitic and cytotoxic activities. The chemical composition of EOP was analyzed using gas chromatography with mass spectrometry (GC-MS). EOP was tested for in vitro activity against Trichomonas vaginalis (ATCC 30236 isolate); trophozoites were treated with different concentrations of EOP (ranging from 25 to 500 μg/mL) in order to establish the MIC and IC50 values...
2018: PloS One
https://www.readbyqxmd.com/read/29380356/association-between-visual-inspection-of-the-cervix-with-acetic-acid-examination-and-high-risk-human-papillomavirus-infection-chlamydia-trachomatis-neisseria-gonorrhoeae-and-trichomonas-vaginalis-in-papua-new-guinea
#17
Andrew J Vallely, Pamela J Toliman, Claire Ryan, Glennis Rai, Johanna Wapling, Josephine Gabuzzi, Antonia Kumbia, Benny Kombuk, Zure Kombati, Lisa M Vallely, Angela Kelly-Hanku, Handan Wand, Sepehr N Tabrizi, Glen D L Mola, John M Kaldor
BACKGROUND: Papua New Guinea (PNG) has among the highest estimated burdens of cervical cancer globally but currently has no national cervical screening program. Visual inspection of the cervix with acetic acid (VIA) is a low-cost screening strategy endorsed by the World Health Organization that has been adopted in many low-resource settings but not previously evaluated in PNG. AIM: To evaluate the association between VIA examination findings and high-risk HPV (hrHPV) infection; and the impact of concomitant genital Chlamydia trachomatis, Neisseria gonorrhoeae and Trichomonas vaginalis on the interpretation of VIA findings...
January 30, 2018: Australian & New Zealand Journal of Obstetrics & Gynaecology
https://www.readbyqxmd.com/read/29376609/-concomitant-urogenital-infections-in-men
#18
O A Chubukova, V V Shkarin
The article presents possible combinations of urogenital infections of various etiologies and some pathogenetic, clinical and epidemiological features, and issues of epidemiological surveillance for co-infection. The authors describe in detail combinations with each other and with other diseases of such pathogens as Chlamydia trachomatis, Ureaplasma urealyticum, Mycoplasma spp., Neisseria gonorrhoeae, Trichomonas vaginalis. They also focus on the problem of co-occurrence of human papillomavirus (HPV) with other urogenital pathogens...
December 2017: Urologii︠a︡
https://www.readbyqxmd.com/read/29368037/mycoplasma-hominis-impacts-gene-expression-in-trichomonas-vaginalis
#19
Ursula Fürnkranz, Birgit Henrich, Julia Walochnik
In Europe, up to 90% of isolated Trichomonas vaginalis strains are naturally infected with Mycoplasma hominis, a facultative pathogen of the human genital tract. The consequences of this endosymbiosis are not yet well understood. The aim of the current study was to evaluate the impact of natural and artificial infections with M. hominis on the RNA expression levels of metronidazole susceptibility-associated genes of T. vaginalis. Three T. vaginalis strains (TVSS10-, TVSS25-, G3) without M. hominis, as well as the same strains naturally (TVSS10+, TVSS25+) and artificially (G3-MhSS25, TVSS25-MhSS25) infected with M...
January 24, 2018: Parasitology Research
https://www.readbyqxmd.com/read/29326178/impact-of-replacing-cytology-with-human-papillomavirus-testing-for-cervical-cancer-screening-on-the-prevalence-of-trichomonas-vaginalis-a-modelling-study
#20
Ben B Hui, Caitlin P Reulein, Rebecca J Guy, Basil Donovan, Jane S Hocking, Matthew G Law, David G Regan
OBJECTIVES: Trichomonas vaginalis (TV) is the most common curable STI worldwide and is associated with increased risk of HIV acquisition and serious reproductive morbidities. The prevalence of TV infection is very low in Australian cities, and this is thought to be at least partly due to incidental detection and treatment of TV in women participating in the cervical cytology screening programme. In 2017, the national cervical screening programme will transition to a new model based on testing for high-risk (HR) human papillomavirus (HPV), with a reduced frequency and commencement at an older age...
January 11, 2018: Sexually Transmitted Infections
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