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https://www.readbyqxmd.com/read/28539504/strategies-for-prevention-and-treatment-of-trichomonas-vaginalis-infections
#1
REVIEW
Kawthar Bouchemal, Christian Bories, Philippe M Loiseau
The last estimated annual incidence of Trichomonas vaginalis worldwide exceeds that of chlamydia and gonorrhea combined. This critical review updates the state of the art on advances in T. vaginalis diagnostics and strategies for treatment and prevention of trichomoniasis. In particular, new data on treatment outcomes for topical administration of formulations are reviewed and discussed.
July 2017: Clinical Microbiology Reviews
https://www.readbyqxmd.com/read/28531212/epidemiological-investigation-of-the-relationship-between-common-lower-genital-tract-infections-and-high-risk-human-papillomavirus-infections-among-women-in-beijing-china
#2
Dai Zhang, Ting Li, Lei Chen, Xiaosong Zhang, Gengli Zhao, Zhaohui Liu
BACKGROUND: The incidence of lower genital tract infections in China has been increasing in recent years. The link between high-risk human papillomavirus (HR-HPV) and other sexually transmitted diseases (STDs) remains unclear. METHODS: From March to October 2014, gynecological examinations and questionnaires were conducted on 1218 married women. Cervical secretions and vaginal swab specimens were tested for Chlamydia trachomatis (CT), Neisseria gonorrhoeae (NG), Ureaplasma urealyticum (UU), yeast, clue cells and HR-HPV...
2017: PloS One
https://www.readbyqxmd.com/read/28525339/molecular-analysis-of-trichomonas-gallinae-in-racing-pigeons-from-upper-silesia-poland
#3
K Bobrek, J Urbanowicz, P Chorbiński, A Gaweł
The aim of the study was the molecular analysis of ITS1/5.8S rRNA/ITS2 region of Trichomonas gallinae isolates from racing pigeon lofts in Upper Silesia, Poland. The analysed region is very useful for the taxonomy of the Trichomonadidae family and indicates the possible existence of different genotypes or species within the T. gallinae. A comparison of the complete ITS1-5.8S-ITS2 region of obtained sequences revealed two different sequences. Twenty-three of the isolates (62%) showed the first sequence (KU954106) while fourteen isolates (38%) showed the second sequence type (KU954107), which were homologous with sequences from Genbank...
March 28, 2017: Polish Journal of Veterinary Sciences
https://www.readbyqxmd.com/read/28521843/high-prevalence-of-trichomonas-gallinae-in-wild-columbids-across-western-and-southern-europe
#4
Melanie Marx, Gerald Reiner, Hermann Willems, Gregorio Rocha, Klaus Hillerich, Juan F Masello, Sylvia L Mayr, Sarah Moussa, Jenny C Dunn, Rebecca C Thomas, Simon J Goodman, Keith C Hamer, Benjamin Metzger, Jacopo G Cecere, Fernando Spina, Steffen Koschkar, Luciano Calderón, Tanja Romeike, Petra Quillfeldt
BACKGROUND: Avian trichomonosis is known as a widespread disease in columbids and passerines, and recent findings have highlighted the pathogenic character of some lineages found in wild birds. Trichomonosis can affect wild bird populations including endangered species, as has been shown for Mauritian pink pigeons Nesoenas mayeri in Mauritius and suggested for European turtle doves Streptopelia turtur in the UK. However, the disease trichomonosis is caused only by pathogenic lineages of the parasite Trichomonas gallinae...
May 18, 2017: Parasites & Vectors
https://www.readbyqxmd.com/read/28511414/a-community-based-study-on-pelvic-inflammatory-disease-in-postmenopausal-females-microbiological-spectrum-and-socio-demographic-correlates
#5
Samreen Khan, M Athar Ansari, Shaista M Vasenwala, Zehra Mohsin
INTRODUCTION: Pelvic Inflammatory Disease (PID) is a common and serious complication of sexually transmitted diseases in young women but is rarely diagnosed in the postmenopausal women. Very few studies have been done regarding post-menopausal PID. AIM: To determine the burden of PID, associated micro-organisms, pathophysiology and risk factors among post-menopausal women of the community. MATERIALS AND METHODS: A cross-sectional study was conducted among 530 post-menopausal women in the registered field practice areas of the JN Medical College and Hospital, Aligarh, Uttar Pradesh, India...
March 2017: Journal of Clinical and Diagnostic Research: JCDR
https://www.readbyqxmd.com/read/28506046/interaction-between-trichomonas-vaginalis-and-the-prostate-epithelium
#6
Jung-Hyun Kim, Ik-Hwan Han, Sang-Su Kim, Soon-Jung Park, Duk-Young Min, Myoung-Hee Ahn, Jae-Sook Ryu
Most men infected with Trichomonas vaginalis are asymptomatic and can remain undiagnosed and untreated. This has been hypothesized to result in chronic persistent prostatic infection. Adhesion of the protozoan organisms to mucosal cells is considered a first and prerequisite step for T. vaginalis infection. Adhesion of T. vaginalis to prostate epithelial cells has not yet been observed; however, there are several reports about inflammation of prostate epithelial cells induced by T. vaginalis. The aim of this study was to investigate whether adhesion and cytotoxicity of T...
April 2017: Korean Journal of Parasitology
https://www.readbyqxmd.com/read/28506033/differential-protein-expressions-in-virus-infected-and-uninfected-trichomonas-vaginalis
#7
Ding He, Gong Pengtao, Yang Ju, Li Jianhua, Li He, Zhang Guocai, Zhang Xichen
Protozoan viruses may influence the function and pathogenicity of the protozoa. Trichomonas vaginalis is a parasitic protozoan that could contain a double stranded RNA (dsRNA) virus, T. vaginalis virus (TVV). However, there are few reports on the properties of the virus. To further determine variations in protein expression of T. vaginalis, we detected 2 strains of T. vaginalis; the virus-infected (V(+)) and uninfected (V(-)) isolates to examine differentially expressed proteins upon TVV infection. Using a stable isotope N-terminal labeling strategy (iTRAQ) on soluble fractions to analyze proteomes, we identified 293 proteins, of which 50 were altered in V(+) compared with V(-) isolates...
April 2017: Korean Journal of Parasitology
https://www.readbyqxmd.com/read/28502874/aerobic-vaginitis-no-longer-a-stranger
#8
Gilbert G G Donders, Gert Bellen, Svitrigaile Grinceviciene, Kateryna Ruban, Pedro Vieira-Baptista
Aerobic vaginitis (AV) is the name given in 2002 to a vaginal infectious entity which was not recognized as such before. It is characterized by abnormal (dysbiotic) vaginal microflora containing aerobic, enteric bacteria, variable levels of vaginal inflammation and deficient epithelial maturation. Although AV and bacterial vaginosis (BV) share some characteristics, such as a diminished number or absence of lactobacilli, increased discharge (fishy smelling in BV, while in severe forms of AV, a foul, rather rotten smell may be present) and increased pH (often more pronounced in AV), there are also striking differences between the two...
May 11, 2017: Research in Microbiology
https://www.readbyqxmd.com/read/28476913/prevalence-and-risk-factors-associated-with-stis-among-women-initiating-contraceptive-implants-in-kingston-jamaica
#9
Margaret Christine Snead, Jeffrey Wiener, Sinmisola Ewumi, Christi Phillips, Lisa Flowers, Tina Hylton-Kong, Natalie Medley-Singh, Jennifer Legardy-Williams, Elizabeth Costenbader, John Papp, Lee Warner, Carolyn Black, Athena P Kourtis
BACKGROUND: There is limited information on rates of STIs in Jamaica due to syndromic management and limited aetiological surveillance. We examined the prevalence of Chlamydia trachomatis (CT), Neisseria gonorrhoeae (NG) and Trichomonas vaginalis (TV) and characteristics associated with STIs among sexually active women who participated in a randomised trial of a progestin implant initiation in Jamaica (the Sino-Implant Study (SIS)). METHODS: SIS was a randomised trial conducted in Kingston, Jamaica, from 2012 to 2014 to evaluate whether initiation of the Sino-Implant (II) led to more unprotected sex among women ages 18-44 years...
May 5, 2017: Sexually Transmitted Infections
https://www.readbyqxmd.com/read/28474007/organelles-that-illuminate-the-origins-of-trichomonas-hydrogenosomes-and-giardia-mitosomes
#10
Michelle M Leger, Martin Kolisko, Ryoma Kamikawa, Courtney W Stairs, Keitaro Kume, Ivan Čepička, Jeffrey D Silberman, Jan O Andersson, Feifei Xu, Akinori Yabuki, Laura Eme, Qianqian Zhang, Kiyotaka Takishita, Yuji Inagaki, Alastair G B Simpson, Tetsuo Hashimoto, Andrew J Roger
No abstract text is available yet for this article.
April 1, 2017: Nat Ecol Evol
https://www.readbyqxmd.com/read/28469158/evaluating-the-utility-of-syndromic-case-management-for-three-sexually-transmitted-infections-in-women-visiting-hospitals-in-delhi-india
#11
Subash Chandra Sonkar, Kirti Wasnik, Anita Kumar, Vineeta Sharma, Pratima Mittal, Prashant Kumar Mishra, Mausumi Bharadwaj, Daman Saluja
Utility of syndromic case management (SCM) in women visiting obstetrics & gynecology department needs to be evaluated as it is subjective and imperfect. Consequently, antibiotic resistance has accelerated along with increased risk of infection to the partners. To understand the effectiveness and/or inadequacies of SCM, 11000 women were recruited and examined by clinicians for infection by Chlamydia trachomatis (CT), Neisseria gonorrhoeae (NG), Trichomonas vaginalis (TV), Bacterial vaginosis (BV) and others...
May 3, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28465215/glycogen-synthase-from-the-parabasalian-parasite-trichomonas-vaginalis-an-unusual-member-of-the-starch-glycogen-synthase-family
#12
Wayne A Wilson, Prajakta Pradhan, Nayasha Madhan, Galen C Gist, Andrew Brittingham
Trichomonas vaginalis, a parasitic protist, is the causative agent of the common sexually-transmitted infection trichomoniasis. The organism has long been known to synthesize substantial glycogen as a storage polysaccharide, presumably mobilizing this compound during periods of carbohydrate limitation, such as might be encountered during transmission between hosts. However, little is known regarding the enzymes of glycogen metabolism in T. vaginalis. We had previously described the identification and characterization of two forms of glycogen phosphorylase in the organism...
April 29, 2017: Biochimie
https://www.readbyqxmd.com/read/28457846/women-with-symptoms-of-a-urinary-tract-infection-but-a-negative-urine-culture-pcr-based-quantification-of-escherichia-coli-suggests-infection-in-most-cases
#13
S Heytens, A De Sutter, L Coorevits, P Cools, J Boelens, L Van Simaey, T Christiaens, M Vaneechoutte, G Claeys
OBJECTIVES: Our objective was to examine whether or not women with symptoms of a urinary tract infection but with a negative culture (20%-30%) do have an infection. METHODS: We performed quantitative PCR (qPCR) for Escherichia coli and Staphylococcus saprophyticus, on top of a standard culture, in urine samples from 220 women with dysuria and/or frequency and/or urgency and from 86 women without symptoms. For symptomatic women, qPCR was also carried out for four sexually transmitted agents...
April 21, 2017: Clinical Microbiology and Infection
https://www.readbyqxmd.com/read/28453175/prevalence-of-human-papillomavirus-chlamydia-trachomatis-and-trichomonas-vaginalis-infections-in-amazonian-women-with-normal-and-abnormal-cytology
#14
E Costa-Lira, A H V L Jacinto, L M Silva, P F R Napoleão, R A A Barbosa-Filho, G J S Cruz, S Astolfi-Filho, C M Borborema-Santos
Sexually transmitted infections are an important cause of morbidity among sexually active women worldwide, and have been implicated as cofactors in the pathogenesis of cervical cancer. We investigated the prevalence of human papillomavirus (HPV), Chlamydia trachomatis (CT), and Trichomonas vaginalis (TV), and accessed the diversity of HPV in women with normal and abnormal cytology in Manaus, Brazil. We used polymerase chain reaction and HPV genotyping by direct sequencing. The chi-square test was used to calculate the absolute and relative frequencies of the categorical variables, and Fisher's test was used when P < 0...
April 28, 2017: Genetics and Molecular Research: GMR
https://www.readbyqxmd.com/read/28442805/proton-pump-inhibitors-are-associated-with-a-reduced-likelihood-for-sexually-transmitted-diseases-in-women-in-the-emergency-department
#15
Johnathan Michael Sheele, Nathan Morris, Donald Byars, Frank Counselman
BACKGROUND: Proton pump inhibitors (PPIs) have been shown in cell culture to kill Trichomonas vaginalis (TV) at lower half maximal inhibitory concentration values than metronidazole (Flagyl), the most common medication used to treat the infection. However, there have been no previous clinical investigations to determine if PPIs are associated with reduced risk for TV. MATERIALS AND METHODS: We examined the records of female patients who received testing in the emergency department for TV, Neisseria gonorrhoea (GC), and Chlamydia trachomatis (CT) between 2010 and 2014 at two academic medical centers to determine if PPI and histamine type 2 receptor antagonist (H2RA) drugs were associated with TV and GC/CT infections...
January 2017: Indian Journal of Sexually Transmitted Diseases
https://www.readbyqxmd.com/read/28430679/cervicovaginal-levels-of-human-%C3%AE-defensin-1-2-3-and-4-of-reproductive-aged-women-with-chlamydia-trachomatis-infection
#16
Nathália Mayumi Noda-Nicolau, Larissa Brito Bastos, Aline Nascimento Bolpetti, Gabriel Vitor Silva Pinto, Larissa Doddi Marcolino, Camila Marconi, Carolina Sanitá Tafner Ferreira, Jossimara Polettini, Eliane Passarelli Vieira, Márcia Guimarães da Silva
OBJECTIVE: This study included women attending primary health care units in Botucatu, São Paulo, Brazil, to assess the cervicovaginal levels of human β-defensin (hBD) 1, 2, 3, and 4 during Chlamydia trachomatis infection. PATIENTS AND METHODS: Cervicovaginal samples were collected for Pap testing and assessing the presence of infection by C. trachomatis, human papillomavirus, Neisseria gonorrhoeae, and Trichomonas vaginalis. Vaginal smears were taken to evaluate local microbiota...
April 21, 2017: Journal of Lower Genital Tract Disease
https://www.readbyqxmd.com/read/28425774/profile-of-the-triplex-assay-for-detection-of-chlamydia-gonorrhea-and-trichomonas-using-the-bd-max%C3%A2-system
#17
Barbara Van Der Pol
Chlamydia, gonorrhea and trichomonas are the most common curable STI. improved access to testing could reduce infection rates and prevent sequelae. nucleic acid amplification tests are the recommend class of diagnostic assay for these infections which are often asymptomatic. Areas covered: A description of the BD MAX™ System (MAX) and the BD MAX CT/GC/TV assay is provided along with data from a large US clinical trial. The capacity of the system for other tests and for lab developed assays is also described...
April 27, 2017: Expert Review of Molecular Diagnostics
https://www.readbyqxmd.com/read/28424786/immunocytochemistry-improving-the-diagnosis-of-trichomonas-vaginalis-infections
#18
Thaisa Helena S Fonseca, Fabrício M Silva Oliveira, Marina Alacoque, Marcella Israel Rocha, Henrique Vitor Leite, Joseph F Guimarães Santos, Haendel G N O Busatti, Marcelo Vidigal Caliari, Maria Aparecida Gomes
The aim of this study was to evaluate the immunocytochemistry (ICC) to diagnose trichomoniasis, particularly asymptomatic infections. By culture serial dilutions, ICC was able to detect 1 trophozoite/mL, while the culture was positive up to 100 trophozoites/mL. The ICC in vivo detection capability was assessed in vaginal secretions of mice experimentally infected and in vaginal swabs from asymptomatic HIV-positive pregnant women compared with culture. All vaginal secretion samples from mice were positive according to both methods...
2017: BioMed Research International
https://www.readbyqxmd.com/read/28419821/expression-of-the-enzymatically-active-legumain-like-cysteine-proteinase-tvlegu-1-of-trichomonas-vaginalis-in-pichia-pastoris
#19
Gerardo Reséndiz-Cardiel, Rossana Arroyo, Jaime Ortega-López
The legumain-like cysteine proteinase TvLEGU-1 from Trichomonas vaginalis plays a major role in trichomonal cytoadherence. However, its structure-function characterization has been limited by the lack of a reliable recombinant expression platform to produce this protein in its native folded conformation. TvLEGU-1 has been expressed in Escherichia coli as inclusion bodies and all efforts to refold it have failed. Here, we describe the expression of the synthetic codon-optimized tvlegu-1 (tvlegu-1-opt) gene in Pichia pastoris strain X-33 (Mut+) under the inducible AOX1 promoter...
April 15, 2017: Protein Expression and Purification
https://www.readbyqxmd.com/read/28413077/trichomonosis-outbreak-in-a-flock-of-canaries-serinus-canaria-f-domestica-caused-by-a-finch-epidemic-strain-of-trichomonas-gallinae
#20
Marko Zadravec, Brigita Slavec, Uroš Krapež, Mitja Gombač, Tanja Švara, Mateja Poljšak-Prijatelj, Igor Gruntar, Joško Račnik
In the present paper, an outbreak of trichomonosis in a flock of 15 breeding pairs of canaries is described. Trichomonosis was diagnosed on characteristic clinical signs, microscopic examination of crop/esophageal swabs, gross pathology and histopathology. Trichomonads were successfully grown in culture media and were characterized by multi-locus sequence typing. The three genomic loci ITS1-5.8S-ITS2, 18S rRNA and Fe-hydrogenase were analyzed. Molecular characterization confirmed the finch trichomonosis strain, identical to the strain that caused emerging disease in free-living passerine birds in Europe...
April 3, 2017: Veterinary Parasitology
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