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fibroblast secretome analysis

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https://www.readbyqxmd.com/read/29425822/microfluidics-for-secretome-analysis-under-enhanced-endogenous-signaling
#1
Qianjiang Hu, Camilla Luni, Nicola Elvassore
Cell secretome, the complex set of proteins that are secreted by the cells, is a fundamental mechanism of cell-cell communication both in vitro and in vivo. In vivo, the analysis of proteins secreted into body fluids can bring to the identification of biomarkers for important physiopathological conditions. However, due to the complexity of the protein content of body fluids, a better understanding of the secreted proteins by different cell types is highly desirable and can be performed in vitro for dissection...
February 6, 2018: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29275043/signatures-of-protein-expression-revealed-by-secretome-analyses-of-cancer-associated-fibroblasts-and-melanoma-cell-lines
#2
Tarcísio Liberato, Dayelle S Pessotti, Isabella Fukushima, Eduardo S Kitano, Solange M T Serrano, André Zelanis
The imbalance of cellular homeostasis during oncogenesis together with the high heterogeneity of tumor-associated stromal cells have a marked effect on the repertoire of the proteins secreted by malignant cells (the secretome). Hence, the study of tumoral secretomes provides insights for understanding the cross-talk between cells within the tumor microenvironment as well as the key effectors for the establishment of the pre-metastatic niche in distant tumor sites. In this context, we performed a proteomic analysis of the secretomes derived from four cell lines: a paired set of fibroblasts - Hs 895...
December 21, 2017: Journal of Proteomics
https://www.readbyqxmd.com/read/29106524/cardiac-myocyte-%C3%AE-3-adrenergic-receptors-prevent-myocardial-fibrosis-by-modulating-oxidant-stress-dependent-paracrine-signaling
#3
Nerea Hermida, Lauriane Michel, Hrag Esfahani, Emilie Dubois-Deruy, Joanna Hammond, Caroline Bouzin, Andreas Markl, Henri Colin, Anne Van Steenbergen, Christophe De Meester, Christophe Beauloye, Sandrine Horman, Xiaoke Yin, Manuel Mayr, Jean-Luc Balligand
Aims: Human and mouse cardiac beta3-adrenergic receptors (beta3AR) exert antipathetic effects to those of beta1-2AR stimulation. We examined their role in modulating myocardial remodelling, particularly fibrosis in response to haemodynamic stress. Methods and results: Mice with cardiac myocyte-specific expression of beta3AR (ADRB3-tg) or tamoxifen-inducible homozygous deletion (c-Adrb3-ko, with loxP-targeted Adrb3) were submitted to transaortic constriction. A superfusion assay was used for proteomic analysis of paracrine mediators between beta3AR-expressing cardiac myocytes and cardiac fibroblasts cultured separately...
July 6, 2017: European Heart Journal
https://www.readbyqxmd.com/read/29101181/endothelial-dysfunction-is-a-super-inducer-of-syndecan-4-fibrogenic-role-of-its-ectodomain
#4
Mark Lipphardt, Jong W Song, Brian B Ratliff, Hassan Dihazi, Gerhard A Müller, Michael S Goligorsky
Syndecan 4 (Synd4) is a member of membrane-spanning, glycocalyx-forming proteoglycan family. It has been suggested that Synd4 participates in renal fibrosis. We compared wild type and fibrosis-prone endothelial sirtuin1-deficient (Sirt1(endo)-/-) mice, the latter being a model of global endothelial dysfunction. We performed mass-spectrometry analysis, which revealed that Synd4 was highly enriched in the secretome of renal microvascular endothelial cells (RMVEC) obtained from Sirt1(endo)-/- mice upon stimulation with TGFβ1; notably all detectable peptides were confined to the ectodomain of Synd4...
November 3, 2017: American Journal of Physiology. Heart and Circulatory Physiology
https://www.readbyqxmd.com/read/28734678/improvement-of-adipose-tissue-derived-cells-by-low-energy-extracorporeal-shock-wave-therapy
#5
Eleni Priglinger, Christina M A P Schuh, Carolin Steffenhagen, Christoph Wurzer, Julia Maier, Sylvia Nuernberger, Wolfgang Holnthoner, Christiane Fuchs, Susanne Suessner, Dominik Rünzler, Heinz Redl, Susanne Wolbank
BACKGROUND: Cell-based therapies with autologous adipose tissue-derived cells have shown great potential in several clinical studies in the last decades. The majority of these studies have been using the stromal vascular fraction (SVF), a heterogeneous mixture of fibroblasts, lymphocytes, monocytes/macrophages, endothelial cells, endothelial progenitor cells, pericytes and adipose-derived stromal/stem cells (ASC) among others. Although possible clinical applications of autologous adipose tissue-derived cells are manifold, they are limited by insufficient uniformity in cell identity and regenerative potency...
July 19, 2017: Cytotherapy
https://www.readbyqxmd.com/read/28713871/immune-dysfunctionality-of-replicative-senescent-mesenchymal-stromal-cells-is-corrected-by-ifn%C3%AE-priming
#6
Raghavan Chinnadurai, Devi Rajan, Spencer Ng, Kenneth McCullough, Dalia Arafat, Edmund K Waller, Larry J Anderson, Greg Gibson, Jacques Galipeau
Industrial-scale expansion of mesenchymal stromal cells (MSCs) is often used in clinical trials, and the effect of replicative senescence on MSC functionality is of mechanistic interest. Senescent MSCs exhibit cell-cycle arrest, cellular hypertrophy, and express the senescent marker β-galactosidase. Although both fit and senescent MSCs display intact lung-homing properties in vivo, senescent MSCs acquire a significant defect in inhibiting T-cell proliferation and cytokine secretion in vitro. IFNγ does not upregulate HLA-DR on senescent MSCs, whereas its silencing did not reverse fit MSCs' immunosuppressive properties...
April 25, 2017: Blood Advances
https://www.readbyqxmd.com/read/28641008/hypoxia-induced-changes-in-the-fibroblast-secretome-exosome-and-whole-cell-proteome-using-cultured-cardiac-derived-cells-isolated-from-neonatal-mice
#7
Jake Cosme, Hongbo Guo, Sina Hadipour-Lakmehsari, Andrew Emili, Anthony O Gramolini
Cardiac fibroblasts (CFs) represent a major subpopulation of cells in the developing and adult heart. Cardiomyocyte (CM) and CF intercellular communication occurs through paracrine interactions and modulate myocyte development and stress response. Detailed proteomic analysis of the CF secretome in normal and stressed conditions may offer insights into the role of CF in heart development and disease. Primary neonatal mouse CFs were isolated and cultured for 24 h in 21% (normoxic) or 2% (hypoxic) O2. Conditioned medium was separated to obtain exosomes (EXO) and EXO-depleted secretome fractions...
July 6, 2017: Journal of Proteome Research
https://www.readbyqxmd.com/read/28542481/effect-of-hyperbaric-oxygen-on-bdnf-release-and-neuroprotection-investigations-with-human-mesenchymal-stem-cells-and-genetically-modified-nih3t3-fibroblasts-as-putative-cell-therapeutics
#8
Jennifer Schulze, Odett Kaiser, Gerrit Paasche, Hans Lamm, Andreas Pich, Andrea Hoffmann, Thomas Lenarz, Athanasia Warnecke
Hyperbaric oxygen therapy (HBOT) is a noninvasive widely applied treatment that increases the oxygen pressure in tissues. In cochlear implant (CI) research, intracochlear application of neurotrophic factors (NTFs) is able to improve survival of spiral ganglion neurons (SGN) after deafness. Cell-based delivery of NTFs such as brain-derived neurotrophic factor (BDNF) may be realized by cell-coating of the surface of the CI electrode. Human mesenchymal stem cells (MSC) secrete a variety of different neurotrophic factors and may be used for the development of a biohybrid electrode in order to release endogenously-derived neuroprotective factors for the protection of residual SGN and for a guided outgrowth of dendrites in the direction of the CI electrode...
2017: PloS One
https://www.readbyqxmd.com/read/28102936/unbiased-and-quantitative-proteomics-reveals-highly-increased-angiogenesis-induction-by-the-secretome-of-mesenchymal-stromal-cells-isolated-from-fetal-rather-than-adult-skin
#9
Massimiliano Gaetani, Cinzia Maria Chinnici, Anna Paola Carreca, Claudia Di Pasquale, Giandomenico Amico, Pier Giulio Conaldi
Scarless wound healing and functional regeneration are typical processes of the fetus, gradually lost during post-natal life, and maximally attributed to fetal skin tissue and induced by fetal skin fibroblasts. The latter have been successfully applied to post-natal wounds, with clear advantages compared with autologous dermis grafts or adult fibroblast applications. Our goal was to functionally identify and uncover key factors and mechanisms through the analysis of secretomes, the principal players in all cell therapies based on mesenchymal stromal cells (MSCs)...
January 19, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/27909049/control-of-secreted-protein-gene-expression-and-the-mammalian-secretome-by-the-metabolic-regulator-pgc-1%C3%AE
#10
Neri Minsky, Robert G Roeder
Secreted proteins serve pivotal roles in the development of multicellular organisms, acting as structural matrix, extracellular enzymes, and signal molecules. However, how the secretome is regulated remains incompletely understood. Here we demonstrate, unexpectedly, that peroxisome proliferator-activated receptor γ coactivator 1-α (PGC-1α), a critical transcriptional co-activator of metabolic gene expression, functions to down-regulate the expression of diverse genes encoding secreted molecules and extracellular matrix components to modulate the secretome...
January 6, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/27797378/stromal-pten-inhibits-the-expansion-of-mammary-epithelial-stem-cells-through-jagged-1
#11
G M Sizemore, S Balakrishnan, A M Hammer, K A Thies, A J Trimboli, J A Wallace, S T Sizemore, R D Kladney, S A Woelke, L Yu, S A Fernandez, A Chakravarti, G Leone, M C Ostrowski
Fibroblasts within the mammary tumor microenvironment are active participants in carcinogenesis mediating both tumor initiation and progression. Our group has previously demonstrated that genetic loss of phosphatase and tensin homolog (PTEN) in mammary fibroblasts induces an oncogenic secretome that remodels the extracellular milieu accelerating ErbB2-driven mammary tumor progression. While these prior studies highlighted a tumor suppressive role for stromal PTEN, how the adjacent normal epithelium transforms in response to PTEN loss was not previously addressed...
April 20, 2017: Oncogene
https://www.readbyqxmd.com/read/27725197/in%C3%A2-vitro-secretomics-study-of-pterygium-derived-fibroblasts-by-itraq-based-quantitative-proteomics-strategy
#12
Aihua Hou, Kai Pong Law, Min Qi Tin, Yoon Pin Lim, Louis Tong
Pterygium is a triangular shaped ocular fibrous surface lesion growing from conjunctiva towards central cornea, causing ocular irritation, astigmatism, and visual disturbance. The condition is characterized by epithelial proliferation, fibrovascular growth, chronic inflammation, and prominent extracellular matrix remodeling. Studies have suggested that aberrant extracellular proteins secreted by fibroblasts lead to abnormal matrix production and tissue invasion contributing to the development of the disease...
December 2016: Experimental Eye Research
https://www.readbyqxmd.com/read/27494767/impact-of-fibroblast-growth-factor-21-on-the-secretome-of-human-perivascular-preadipocytes-and-adipocytes-a-targeted-proteomics-approach
#13
Lucia Berti, Sonja Hartwig, Martin Irmler, Bernhard Rädle, Dorothea Siegel-Axel, Johannes Beckers, Stefan Lehr, Hadi Al-Hasani, Hans-Ulrich Häring, Martin Hrabě de Angelis, Harald Staiger
CONTEXT: Perivascular adipose tissue (PVAT) is suggested to impact on vascular cells via humoral factors, possibly contributing to endothelial dysfunction and atherosclerosis. OBJECTIVE: To address whether the hepatokine fibroblast growth factor (FGF) 21 affects the PVAT secretome. METHODS: Human perivascular (pre)adipocytes were subjected to targeted proteomics and whole-genome gene expression analysis. RESULTS: Preadipocytes, as compared to adipocytes, secreted higher amounts of inflammatory cytokines and chemokines...
December 2016: Archives of Physiology and Biochemistry
https://www.readbyqxmd.com/read/27468573/circulating-reticulocalbin-1-and-reticulocalbin-3-in-systemic-sclerosis-patients-results-of-a-case-control-study
#14
Paul Bălănescu, Anca Lădaru, Eugenia Bălănescu, Valer Pompilian, Daniela Gologanu, Simona Caraiola, Cristian Băicuş, Gheorghe A Dan
BACKGROUND: Proteomic candidate biomarkers for systemic sclerosis (Ssc) useful for appropriate patient evaluation and follow-up were identified in mass-spectrometry studies; however, most of these biomarkers were not evaluated and confirmed on independent patient samples. Up-regulation of reticulocalbin 1 (RCN1) and reticulocalbin 3 (RCN3) in the dermal fibroblast secretome originating from Ssc patients was previously described. The aim of the study was to evaluate circulating RCN1 and RCN3 as candidate biomarkers for Ssc clinical expression...
2016: Clinical Laboratory
https://www.readbyqxmd.com/read/27393124/post-invasion-events-after-infection-with-staphylococcus-aureus-are-strongly-dependent-on-both-the-host-cell-type-and-the-infecting-s-%C3%A2-aureus-strain
#15
M Strobel, H Pförtner, L Tuchscherr, U Völker, F Schmidt, N Kramko, H-J Schnittler, M J Fraunholz, B Löffler, G Peters, S Niemann
Host cell invasion is a major feature of Staphylococcus aureus and contributes to infection development. The intracellular metabolically active bacteria can induce host cell activation and death but they can also persist for long time periods. In this study a comparative analysis was performed of different well-characterized S. aureus strains in their interaction with a variety of host cell types. Staphylococcus aureus (strains 6850, USA300, LS1, SH1000, Cowan1) invasion was compared in different human cell types (epithelial and endothelial cells, keratinocytes, fibroblasts, osteoblasts)...
September 2016: Clinical Microbiology and Infection
https://www.readbyqxmd.com/read/27322879/stanniocalcin-1-expression-in-normal-human-endometrium-and-dysregulation-in-endometriosis
#16
MULTICENTER STUDY
Lusine Aghajanova, Signe Altmäe, Sergo Kasvandik, Andres Salumets, Anneli Stavreus-Evers, Linda C Giudice
OBJECTIVE: To determine expression of stanniocalcin-1 (STC1) in human endometrium with and without endometriosis and its regulation by steroid hormones. DESIGN: Laboratory study. SETTING: University. PATIENT(S): Nineteen women with endometriosis and 33 control women. INTERVENTION(S): Endometrial biopsy and fluid sampling. MAIN OUTCOME MEASURE(S): Analysis of early secretory (ESE) and midsecretory (MSE) endometrial secretomes from fertile women with the use of nano-liquid chromatography-dual mass spectrometry; real-time quantitative polymerase chain reaction, and immunohistochemistry for STC1 and its receptor calcium-sensing receptor (CASR) mRNA and proteins in endometrium with and without endometriosis; evaluation of STC1 and CASR mRNA expression in endometrial stromal fibroblasts (eSF) from women with and without endometriosis decidualized with the use of E2P or 8-bromo-cyclic adenosine monophosphate (cAMP)...
September 1, 2016: Fertility and Sterility
https://www.readbyqxmd.com/read/27199661/erratum-an-optimized-approach-to-recover-secreted-proteins-from-fibroblast-conditioned-media-for-secretomic-analysis
#17
(no author information available yet)
[This corrects the article on p. 70 in vol. 10, PMID: 27064649.].
2016: Frontiers in Cellular Neuroscience
https://www.readbyqxmd.com/read/27064649/an-optimized-approach-to-recover-secreted-proteins-from-fibroblast-conditioned-media-for-secretomic-analysis
#18
Bastien Paré, Lydia T Deschênes, Roxane Pouliot, Nicolas Dupré, Francois Gros-Louis
The proteins secreted by a particular type of cell, the secretome, play important roles in the regulation of many physiological processes via paracrine/autocrine mechanisms, and they are of increasing interest to help understanding rare diseases and to identify potential biomarkers and therapeutic targets. To facilitate ongoing research involving secreted proteins, we revisited cell culture protocols and whole secreted protein enrichment protocols. A reliable method for culturing and precipitating secreted protein from patient-derived fibroblast conditioned-medium was established...
2016: Frontiers in Cellular Neuroscience
https://www.readbyqxmd.com/read/26892343/combined-secretomics-and-transcriptomics-revealed-cancer-derived-gdf15-is-involved-in-diffuse-type-gastric-cancer-progression-and-fibroblast-activation
#19
Takayuki Ishige, Motoi Nishimura, Mamoru Satoh, Mai Fujimoto, Masaki Fukuyo, Toshihisa Semba, Sayaka Kado, Sachio Tsuchida, Setsu Sawai, Kazuyuki Matsushita, Akira Togawa, Hisahiro Matsubara, Atsushi Kaneda, Fumio Nomura
Gastric cancer is classified into two subtypes, diffuse and intestinal. The diffuse-type gastric cancer (DGC) has poorer prognosis, and the molecular pathology is not yet fully understood. The purpose of this study was to identify functional secreted molecules involved in DGC progression. We integrated the secretomics of six gastric cancer cell lines and gene expression analysis of gastric cancer tissues with publicly available microarray data. Hierarchical clustering revealed characteristic gene expression differences between diffuse- and intestinal-types...
February 19, 2016: Scientific Reports
https://www.readbyqxmd.com/read/26762409/secretome-profiling-of-oral-squamous-cell-carcinoma-associated-fibroblasts-reveals-organization-and-disassembly-of-extracellular-matrix-and-collagen-metabolic-process-signatures
#20
Elizabete Bagordakis, Iris Sawazaki-Calone, Carolina Carneiro Soares Macedo, Carolina M Carnielli, Carine Ervolino de Oliveira, Priscila Campioni Rodrigues, Ana Lucia C A Rangel, Jean Nunes Dos Santos, Juha Risteli, Edgard Graner, Tuula Salo, Adriana Franco Paes Leme, Ricardo D Coletta
An important role has been attributed to cancer-associated fibroblasts (CAFs) in the tumorigenesis of oral squamous cell carcinoma (OSCC), the most common tumor of the oral cavity. Previous studies demonstrated that CAF-secreted molecules promote the proliferation and invasion of OSCC cells, inducing a more aggressive phenotype. In this study, we searched for differences in the secretome of CAFs and normal oral fibroblasts (NOF) using mass spectrometry-based proteomics and biological network analysis. Comparison of the secretome profiles revealed that upregulated proteins involved mainly in extracellular matrix organization and disassembly and collagen metabolism...
July 2016: Tumour Biology: the Journal of the International Society for Oncodevelopmental Biology and Medicine
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