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Lijing Liu, Hong Qian, Hua Xiao, Jianbin He, Maofeng Xie, Zaiyan Wang, Xingyun Long
Objective To explore the role of transforming growth factor-β1 (TGF-β1)/a disintegrin-like and metalloproteinase with thrombospondin type 1 motif (ADAMTS-1) signaling pathway in emodin's anti-pulmonary fibrosis. Methods Sixty SD rats were randomly divided into 6 groups: normal control group, sham-operated group, model group, low-dose emodin intervention group (20 mg/kg), high-dose emodin intervention group (80 mg/kg) and prednisone group (5 mg/kg). Each group included 10 animals. Rats in the latter 4 groups were intratracheally injected with bleomycin A5 to induce pulmonary fibrosis, whereas bleomycin A5 was replaced by normal saline in sham-operated group...
October 2016: Xi Bao Yu Fen Zi Mian Yi Xue za Zhi, Chinese Journal of Cellular and Molecular Immunology
S Balachandar, M Sethuram, P Muthuraja, T Shanmugavadivu, M Dhandapani
Biologically active Lewis acid-base compound, pyrazolium 3-nitro phthalate (P3NP) has been synthesized and crystallized by slow evaporation - solution method at 30°C. Spectral and single crystal X-Ray diffraction (XRD) were used to characterize the compound. The stability of the P3NP was confirmed by UV-Visible spectral analysis. P3NP crystallizes in monoclinic P21/C space group with cell parameters, a=13.009 (3) Å, b=12.584 (3) Å, c=7.529 (18) Å and β=93.052 (4)(o) with Z=4. Crystal packing was stabilized by N(+)H⋯O(-), OH⋯O and CH⋯O intermolecular hydrogen bonds...
October 2016: Journal of Photochemistry and Photobiology. B, Biology
Rasha Tarif Hamza, Alaa Youssef Ahmed, Doaa Gamal Rezk, Amira Ibrahim Hamed
BACKGROUND: Excessive use of fructose has been incriminated as a risk factor for hepatic steatosis. Procollagen type III N-terminal peptide (P3NP) is a marker for steatohepatitis. Thus, we aimed to assess fructose intake in obese children and its relation to nonalcoholic fatty liver disease (NAFLD) and P3NP. METHODS: Fifty-five obese children were compared to 30 controls. All were subjected to dietary fructose and anthropometric assessment, fasting blood sugar (FBS), fasting insulin (FI) and homeostasis model assessment of insulin resistance (HOMA-IR), lipid profile, uric acid, alanine aminotransferase (ALT), P3NP and abdominal ultrasound...
July 21, 2016: Journal of Pediatric Endocrinology & Metabolism: JPEM
Ilaria Barchetta, Maria Del Ben, Francesco Angelico, Michele Di Martino, Antonio Fraioli, Giuseppe La Torre, Rosella Saulle, Ludovica Perri, Sergio Morini, Claudio Tiberti, Laura Bertoccini, Flavia Agata Cimini, Francesca Panimolle, Carlo Catalano, Marco Giorgio Baroni, Maria Gisella Cavallo
BACKGROUND: Non-alcoholic fatty liver disease (NAFLD) is the most common hepatic disorder worldwide, reaching prevalence up to 90 % in obese patients with type 2 diabetes (T2D), and representing an independent risk factor for cardiovascular mortality. Furthermore, the coexistence of T2D and NAFLD leads to higher incidence of diabetes' complications and additive detrimental liver outcomes. The existence of a close association between NAFLD and hypovitaminosis D, along with the anti-inflammatory and insulin-sensitizing properties of vitamin D, have been largely described, but vitamin D effects on hepatic fat content have never been tested in a randomized controlled trial...
June 29, 2016: BMC Medicine
C M Maybury, E Samarasekera, A Douiri, J N Barker, C H Smith
People with psoriasis taking methotrexate may be at increased risk of developing liver fibrosis compared with the general population. Noninvasive methods of detecting fibrosis have been widely adopted but their clinical utility is uncertain. To evaluate the diagnostic accuracy of noninvasive methods to detect fibrosis compared with liver biopsy (reference standard) in people with psoriasis taking methotrexate. A systematic search using Ovid/Medline, Embase, Cumulative Index to Nursing and Allied Health Literature, the Cochrane Library and Clinical Trials Register was performed...
June 2014: British Journal of Dermatology
Sarah D Berry, Vasan S Ramachandran, Peggy M Cawthon, Philimon Gona, Robert R McLean, L Adrienne Cupples, Douglas P Kiel
BACKGROUND: Procollagen type III N-terminal peptide (P3NP) is released during collagen synthesis in muscle. Increased circulating P3NP is a marker not only of muscle growth, but also of muscle repair and fibrosis. Thus, P3NP may be a potential biomarker for sarcopenia. OBJECTIVE: To determine the association between plasma P3NP and lean mass and strength. DESIGN SETTING AND PARTICIPANTS: A cross-sectional study of men and women from the Framingham Offspring Study...
2013: Journal of Frailty & Aging
Maren S Fragala, Adam R Jajtner, Kyle S Beyer, Jeremy R Townsend, Nadia S Emerson, Tyler C Scanlon, Leonardo P Oliveira, Jay R Hoffman, Jeffrey R Stout
BACKGROUND: N-terminal peptide of procollagen type III (P3NP) and C-terminal agrin fragment (CAF) are circulating biomarkers that are related to lean body mass in older adults. P3NP is a circulating marker reflective of muscular structural remodeling while CAF is a circulating marker of neuromuscular remodeling. As resistance exercise is an established intervention that can effectively improve muscle quality, we sought to evaluate circulating biomarker changes corresponding to a resistance exercise intervention in older adults...
June 2014: Journal of Cachexia, Sarcopenia and Muscle
Diana J Leeming, Inger Byrjalsen, Wladimiro Jiménez, Claus Christiansen, Morten A Karsdal
BACKGROUND/AIM: We investigated nine novel biomarkers of extracellular matrix (ECM) remodelling in a rat model of liver fibrosis. METHODS: Liver fibrosis was induced in 52 male Wistar rats by inhalation of carbon tetrachloride and the level of hepatic fibrosis was assessed by Sirius red staining compared with controls. The novel serum biochemical markers assessed in the model were type I-(C1M), type III-(C3M), type IV-(C4M) and type VI-(C6M) collagen, citrullinated vimentin (VICM) and biglycan (BGM) all protein fragments generated by matrix metalloproteinases; and formation markers of type III-(P3NP), type VI (P4NP 7S) and type V (P5CP) collagen; hepatic mRNA type I collagen alpha-1 chain levels, serum potassium, sodium, osmolarity, alanine aminotransferase, lactate dehydrogenase, albumin and creatinine...
March 2013: Liver International: Official Journal of the International Association for the Study of the Liver
Anne Sofie Siebuhr, Jianxia Wang, Morten Karsdal, Anne-C Bay-Jensen, Jin Y, Zheng Q
BACKGROUND: Rheumatoid arthritis is a disease affecting the extracellular matrix of especially synovial joints. The thickness of the synovial membrane increases and surrounding tissue degrades, leading to altered collagen balance in the tissues. In this study, we investigated the altered tissue balance of cartilage, synovial membrane, and connective tissue in collagen induced arthritis (CIA) in rats. METHODS: Six newly developed ELISAs quantifying MMP-derived collagen degradation (C1M, C2M, and C3M) and formation (P1NP, P2NP, and P3NP) was used to detect cartilage turnover in rats with CIA...
2012: Journal of Translational Medicine
Nick Sheron, Mike Moore, Stacey Ansett, Camille Parsons, Adrian Bateman
BACKGROUND: Liver disease develops silently and presents late, with often fatal complications. AIM: To develop a 'traffic light' test for liver disease suitable for community use that could enhance assessment of liver risk and allow rational referral of more severe disease to specialist care. DESIGN AND SETTING: Two cohorts from Southampton University Hospital Trust Liver Unit: model development and a validation cohort to evaluate prognosis...
September 2012: British Journal of General Practice: the Journal of the Royal College of General Practitioners
E Y H Khoo, M C Stevenson, E Leverton, R Cross, J W Eriksson, S M Poucher, I Spendlove, P G Morris, I A Macdonald, P Mansell, G P Aithal
BACKGROUND: Hyperalimentation for 4 weeks is associated with raised liver enzymes and liver fat content (LFC), which are two common features found in individuals with diabetes. AIM: We evaluated the effect of two mixed meal challenges on LFC, liver enzymes and serum bio-markers of liver injury and fibrosis in 16 healthy volunteers (HV) and subjects with type 2 diabetes (T2DM). METHODS: Subjects (HV: 9 male, 7 female, aged 57.9 ± 1.7 years, body mass index (BMI) 27...
November 2012: Digestive Diseases and Sciences
Indra Neil Guha, Sumit Bokhandi, Zaheer Ahmad, Nick Sheron, Richard Cope, Caroline Marshall, Gruschen Veldtman
BACKGROUND: The liver is known to be structurally abnormal in long-standing Fontan circulation. The degree of liver dysfunction associated with such abnormalities is however largely unknown. We assessed structural changes (serum fibrosis markers) and function (indocyanine green clearance (ICG)) in Fontan patients. METHODS: 21 stable Fontan patients were prospectively assessed and compared with 8 histologically proven compensated viral cirrhotic patients. All subjects had standard liver profile, "Enhanced Liver Fibrosis" (ELF) score (including hyaluronic acid, aminoterminal type III procollagen peptide P3NP and tissue inhibitor of metalloproteinase TIMP-1 levels), and ICG using the LiMON Device...
March 20, 2013: International Journal of Cardiology
Fabian Chen, Raymond Lam, David Shaywitz, Ronald C Hendrickson, Gregory J Opiteck, Dana Wishengrad, Andy Liaw, Qinghua Song, Adrian J Stewart, Corinne E Cummings, Chan Beals, Kevin E Yarasheski, Alise Reicin, Marcella Ruddy, Xuguang Hu, Nathan A Yates, Joseph Menetski, Gary A Herman
BACKGROUND: Early biomarkers of skeletal muscle anabolism will facilitate the development of therapies for sarcopenia and frailty. METHODS AND RESULTS: We examined plasma type III collagen N-terminal propeptide (P3NP), skeletal muscle protein fractional synthesis rate, and gene and protein expression profiles to identify testosterone-induced changes in muscle anabolism. Two placebo-controlled studies enrolled community-dwelling men (study 1, 60-75 years; study 2, 18-40 years) with low to normal testosterone levels...
March 2011: Journal of Cachexia, Sarcopenia and Muscle
Shalender Bhasin, E Jiaxiu He, Miwa Kawakubo, E Todd Schroeder, Kevin Yarasheski, Gregory J Opiteck, Alise Reicin, Fabian Chen, Raymond Lam, Jeffrey A Tsou, Carmen Castaneda-Sceppa, Ellen F Binder, Stanley P Azen, Fred R Sattler
CONTEXT: Biomarkers that predict musculoskeletal response to anabolic therapies should expedite drug development. During collagen synthesis in soft lean tissue, N-terminal propeptide of type III procollagen (P3NP) is released into circulation. We investigated P3NP as a biomarker of lean body mass (LBM) and muscle strength gains in response to testosterone and GH. DESIGN: Community-dwelling older men received GnRH agonist plus 5 or 10 g testosterone gel plus 0, 3, or 5 microg recombinant human GH daily...
November 2009: Journal of Clinical Endocrinology and Metabolism
S Khan, D Subedi, M M U Chowdhury
Hepatic fibrosis continues to be a risk in patients receiving methotrexate for psoriasis. Measurement of amino terminal levels of type III procollagen (P3NP) has been advocated as an effective non-invasive test for ongoing hepatic fibrogenesis that could avoid liver biopsies. An audit was conducted to assess the practice of P3NP monitoring using guidelines produced by Manchester and whether the agreed levels correlate with histological severity. Sixty five patients with 174 P3NP assays and 30 liver biopsies were reviewed between the years 1999 and 2003...
May 2006: Postgraduate Medical Journal
Louise F Bath, Patricia M Crofton, Agnes E M Evans, Michael B Ranke, Martin W Elmlinger, Christopher J H Kelnar, W Hamish B Wallace
Children who are treated for malignancy have been shown to have decreased bone mineral density. We investigated the effect of serial courses of chemotherapy on growth and bone turnover in children with solid tumors. We measured height, weight, and lower leg length (LLL; n = 10) and markers of bone formation [bone alkaline phosphatase (BALP) and C-terminal propeptide of type I collagen (P1CP)], bone resorption [C terminal telopeptide of type I collagen (1CTP)], soft tissue collagen turnover [N-terminal propeptide of type III procollagen (P3NP)], and the GH axis [IGF1 and its binding proteins (IGFBP3 and IGFBP2)] before and after each course (n = 25) and on completion of treatment (n = 12)...
February 2004: Pediatric Research
P M Crofton, S F Ahmed, J C Wade, M W Elmlinger, M B Ranke, C J Kelnar, W H Wallace
Children treated for acute lymphoblastic leukemia may develop reduced bone mineral density during treatment, but there is little information on the mechanisms involved. In a prospective, longitudinal study on 15 children with ALL, we undertook serial measurements of markers of bone and collagen turnover, insulin-like growth factor (IGF)-I and its binding proteins (IGFBPs)-3 and -2 during the second year of continuing chemotherapy. In eight patients we also measured lower leg length by knemometry. Height SD scores, lower leg length velocity, IGF-I, and markers of bone collagen turnover did not differ significantly from healthy children...
October 2000: Pediatric Research
P M Crofton, A Shrivastava, J C Wade, R Stephen, Kelnar CJH, N Mcintosh, A J Lyon
Dexamethasone is used commonly in the treatment of chronic lung disease of prematurity, but there are concerns about possible deleterious effects on growth and bone. Our aim in this study was to examine the effects of dexamethasone treatment on bone and collagen turnover in preterm infants. Bone-specific alkaline phosphatase, the C-terminal propeptide of type I collagen (PICP, reflecting whole-body type I collagen synthesis), and the N-terminal propeptide of type III procollagen (P3NP, reflecting soft tissue collagen turnover), together with the C-terminal telopeptide of type I collagen (ICTP), urinary pyridinoline (Pyd), and deoxypyridinoline (all markers of collagen breakdown) were measured at weekly intervals over the first 12 wk of life in 14 preterm infants with chronic lung disease treated with dexamethasone...
August 2000: Pediatric Research
P M Crofton, A Shrivastava, J C Wade, R Stephen, C J Kelnar, A J Lyon, N McIntosh
In a longitudinal study of 25 preterm infants, we have examined the relationship of bone-specific alkaline phosphatase (ALP), C-terminal propeptide of type I collagen (PICP), N-terminal propeptide of type III procollagen (P3NP), C-terminal telopeptide of type I collagen, urinary pyridinoline (Pyd) and deoxypyridinoline (Dpd), with rates of gain in weight, length, and lower leg length and with bone mineral content (BMC), all measured at weekly intervals over the first 10 wk of life. Concentrations of all collagen markers were 10-fold higher than in older children...
November 1999: Pediatric Research
P M Crofton, S F Ahmed, J C Wade, R Stephen, M W Elmlinger, M B Ranke, C J Kelnar, W H Wallace
To investigate the effects of disease and intensive chemotherapy on bone turnover and growth in children with acute lymphoblastic leukemia (ALL), a longitudinal prospective study was carried out in 22 children, aged 1.2-13.5 yr, enrolled in the Medical Research Council-funded randomized trial of childhood ALL treatment in the UK. We measured lower leg length and markers of bone formation [bone alkaline phosphatase (ALP) and procollagen type I C-terminal propeptide (PICP)], bone resorption [pyridinoline, deoxypyridinoline, and carboxyl-terminal telopeptide of type I collagen (ICTP)], soft tissue turnover [procollagen type III N-terminal propeptide (P3NP)], and the GH axis [IGF-I, IGF-binding protein-3 (IGFBP-3), IGFBP-2, and urinary GH] at 1- to 4-week intervals from diagnosis to week 27 of treatment...
September 1998: Journal of Clinical Endocrinology and Metabolism
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