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secretome analysis

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https://www.readbyqxmd.com/read/28102936/unbiased-and-quantitative-proteomics-reveals-highly-increased-angiogenesis-induction-by-the-secretome-of-mesenchymal-stromal-cells-isolated-from-fetal-rather-than-adult-skin
#1
Massimiliano Gaetani, Cinzia Maria Chinnici, Anna Paola Carreca, Claudia Di Pasquale, Giandomenico Amico, Pier Giulio Conaldi
Scarless wound healing and functional regeneration are typical processes of the fetus, gradually lost during post-natal life, and maximally attributed to fetal skin tissue and induced by fetal skin fibroblasts. The latter have been successfully applied to post-natal wounds, with clear advantages compared with autologous dermis grafts or adult fibroblast applications. Our goal was to functionally identify and uncover key factors and mechanisms through the analysis of secretomes, the principal players in all cell therapies based on mesenchymal stromal cells (MSCs)...
January 19, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28087649/identification-of-unique-essential-proteins-from-a-m-tuberculosis-f15-lam4-kzn-phage-secretome-library
#2
Thamsanqa Emmanuel Chiliza, Manormoney Pillay, Balakrishna Pillay
Mycobacterium tuberculosis (Mtb) is the causative agent of tuberculosis disease (TB), the leading cause of death from bacterial infection worldwide. Although treatable, the resurgence of multi- and extensively-drug resistance TB is a major setback for the fight against TB globally. Consequently, there is an urgent need for new Mtb derived biomarkers for use in the design of new drugs and rapid point-of-care diagnostic or prognostic tools for management of TB transmission. Therefore, the present study aimed to identify unique Mtb secreted proteins from the extensively drug resistant Mtb F15/LAM4/KZN phage secretome library...
January 12, 2017: Pathogens and Disease
https://www.readbyqxmd.com/read/28070933/secretome-analysis-of-diarrhea-inducing-strains-of-escherichia-coli
#3
Raja Sekhar Nirujogi, Babylakshmi Muthusamy, Min-Sik Kim, Gajanan J Sathe, P T V Lakshmi, Olga N Kovbasnjuk, T S Keshava Prasad, Mary Wade, Rabih E Jabbour
Secreted proteins constitute a major part of virulence factors that are responsible for pathogenesis caused by gram negative bacteria. Enterohemorrhagic Escherichia coli (E. coli), EHEC O157:H7 is the major pathogen often causing outbreaks. However, studies have reported that the significant outbreaks caused by non O157:H7 E. coli strains, also known as "Big-Six" serogroup strains are increasing. There is no systematic study describing differential secreted proteins from these non-O157:H7 E. coli strains. In this study, we carried out mass spectrometry-based differential secretome analysis using tandem mass tags labeling strategy of non-O157:H7 E...
January 9, 2017: Proteomics
https://www.readbyqxmd.com/read/28063828/genome-wide-computational-analysis-of-the-secretome-of-brown-algae-phaeophyceae
#4
Makoto Terauchi, Takahiro Yamagishi, Takeaki Hanyuda, Hiroshi Kawai
Brown algae have evolved complex multicellularity in the heterokont lineage. They are phylogenetically distant to land plants, fungi and animals. Especially, the members of Laminariales (so-called kelps) have developed highly differentiated tissues. Extracellular matrix (ECM) plays pivotal roles in a number of essential processes in multicellular organisms, such as cell adhesion, cell and tissue differentiations, cell-to-cell communication, and responses to environmental stimuli. In these processes, a set of extracellular secreted proteins called the secretome operates remodeling of the physicochemical nature of ECM and signal transduction by interacting with cell surface proteins and signaling molecules...
January 4, 2017: Marine Genomics
https://www.readbyqxmd.com/read/28060763/comparison-of-the-tumor-cell-secretome-and-patient-sera-for-an-accurate-serum-based-diagnosis-of-pancreatic-ductal-adenocarcinoma
#5
Shakhawan Mustafa, Longqiang Pan, Aseel Marzoq, Malak Fawaz, Laureen Sander, Felix Rückert, Andrea Schrenk, Christina Hartl, Rico Uhler, Adem Yildirim, Oliver Strobel, Thilo Hackert, Nathalia Giese, Markus W Büchler, Jörg D Hoheisel, Mohamed Saiel Saeed Alhamdani
Pancreatic cancer is the currently most lethal malignancy. Toward an accurate diagnosis of the disease in body liquids, we studied the protein composition of the secretomes of 16 primary and established cell lines of pancreatic ductal adenocarcinoma (PDAC). Compared to the secretome of non-tumorous cells, 112 proteins exhibited significantly different abundances. Functionally, the proteins were associated with PDAC features, such as decreased apoptosis, better cell survival and immune cell regulation. The result was compared to profiles obtained from 164 serum samples from two independent cohorts - a training and a test set - of patients with PDAC or chronic pancreatitis and healthy donors...
January 2, 2017: Oncotarget
https://www.readbyqxmd.com/read/28053006/co-option-and-de-novo-gene-evolution-underlie-molluscan-shell-diversity
#6
Felipe Aguilera, Carmel McDougall, Bernard M Degnan
Molluscs fabricate shells of incredible diversity and complexity by localized secretions from the dorsal epithelium of the mantle. Although distantly-related molluscs express remarkably different secreted gene products, it remains unclear if the evolution of shell structure and pattern is underpinned by the differential co-option of conserved genes or the integration of lineage-specific genes into the mantle regulatory program. To address this, we compare the mantle transcriptomes of 11 bivalves and gastropods of varying relatedness...
January 4, 2017: Molecular Biology and Evolution
https://www.readbyqxmd.com/read/28035653/non-classical-monocytes-levels-increased-by-subcutaneous-fat-secretome-are-associated-with-less-rehospitalization-after-heart-failure-admission
#7
Sonia Eiras, Alfonso Varela-Román, Mariléia Cháves Andrade, Ana Castro, Rocío González-Ferreiro, Juan E Viñuela, Ángel Fernández-Trasancos, Marcos C Carreira, Ezequiel Álvarez, Felipe F Casanueva, José R González-Juanatey
Differential monocyte subsets are increased in obesity and heart failure (HF). We studied their role as predictors of rehospitalization for HF and their regulation by adipose tissue. Monocyte subsets and body fat composition were determined from 136 patients at the discharge after HF admission. Regulation of monocytes by SAT secretomes from obese/non-obese patients with HF was studied in a cell culture method. Proteomic analysis of secretome SAT was performed by LC-MALDI TOF/TOF. High CD14(-)CD16(+) monocyte levels indicated less rehospitalization for HF (p = 0...
December 29, 2016: Journal of Cardiovascular Translational Research
https://www.readbyqxmd.com/read/28010772/characterization-of-clostridium-thermocellum-b8-secretome-and-purified-cellulosomes-for-lignocellulosic-biomass-degradation
#8
Karen O Osiro, Brenda R de Camargo, Rachel Satomi, Pedro Ricardo V Hamann, Jéssica Pinheiro Silva, Marcelo Valle de Sousa, Betania F Quirino, Elaine N Aquino, Carlos R Felix, André Melro Murad, Eliane F Noronha
The main goal of the present study was a complete proteomic characterization of total proteins eluted from residual substrate-bound proteins (RSBP), and cellulosomes secreted by Clostridium thermocellum B8 during growth in the presence of microcrystalline cellulose as a carbon source. The second goal was to evaluate their potential use as enzymatic blends for hydrolyzing agro-industrial residues to produce fermentable sugars. Protein identification through LC-MS/MS mass spectrometry showed that the RSBP sample, in addition to cellulosomal proteins, contains a wide variety of proteins, including those without a well-characterized role in plant cell wall degradation...
February 2017: Enzyme and Microbial Technology
https://www.readbyqxmd.com/read/28003119/secretomes-of-mycoplasma-hyopneumoniae-and-mycoplasma-flocculare-reveal-differences-associated-to-pathogenesis
#9
Jéssica A Paes, Karina R Lorenzatto, Sofia N de Moraes, Hercules Moura, John R Barr, Henrique B Ferreira
: Mycoplasma hyopneumoniae and Mycoplasma flocculare cohabit the porcine respiratory tract. However, M. hyopneumoniae causes the porcine enzootic pneumonia, while M. flocculare is a commensal bacterium. Comparative analyses demonstrated high similarity between these species, which includes the sharing of all predicted virulence factors. Nevertheless, studies related to soluble secretomes of mycoplasmas were little known, although they are important for bacterial-host interactions. The aim of this study was to perform a comparative analysis between the soluble secreted proteins repertoires of the pathogenic Mycoplasma hyopneumoniae and its closely related commensal Mycoplasma flocculare...
December 18, 2016: Journal of Proteomics
https://www.readbyqxmd.com/read/27991726/an-optimized-version-of-the-secretome-protein-enrichment-with-click-sugars-specs-method-leads-to-enhanced-coverage-of-the-secretome
#10
Alperen Serdaroglu, Stephan A Müller, Ute Schepers, Stefan Bräse, Stefan F Lichtenthaler, Peer-Hendrik Kuhn
The secretome, the entirety of all soluble proteins either being secreted or proteolytically released by a cell, plays a key role in intercellular communication of multicellular organisms. Pathological alterations contribute to diseases such as hypertension, cancer, autoimmune disorders or neurodegenerative diseases. Hence, studying disease related perturbations of the secretome and the secretome itself covers an important aspect of cellular physiology. We recently developed the Secretome Protein Enrichment with Click Sugars (SPECS) method which enables the analysis of secretomes of in vitro cell cultures even in the presence of fetal calf serum with mass spectrometry...
December 19, 2016: Proteomics
https://www.readbyqxmd.com/read/27984345/vulvodynia-what-we-know-and-where-we-should-be-going
#11
Logan M Havemann, David R Cool, Pascal Gagneux, Michael P Markey, Jerome L Yaklic, Rose A Maxwell, Ashvin Iyer, Steven R Lindheim
OBJECTIVE: The aim of the study was to review the current nomenclature and literature examining microbiome cytokine, genomic, proteomic, and glycomic molecular biomarkers in identifying markers related to the understanding of the pathophysiology and diagnosis of vulvodynia (VVD). MATERIALS AND METHODS: Computerized searches of MEDLINE and PubMed were conducted focused on terminology, classification, and "omics" variations of VVD. Specific MESH terms used were VVD, vestibulodynia, metagenomics, vaginal fungi, cytokines, gene, protein, inflammation, glycomic, proteomic, secretomic, and genomic from 2001 to 2016...
December 15, 2016: Journal of Lower Genital Tract Disease
https://www.readbyqxmd.com/read/27977806/integrated-activity-and-genetic-profiling-of-secreted-peptidases-in-cryptococcus-neoformans-reveals-an-aspartyl-peptidase-required-for-low-ph-survival-and-virulence
#12
Starlynn C Clarke, Phillip A Dumesic, Christina M Homer, Anthony J O'Donoghue, Florencia La Greca, Lenka Pallova, Pavel Majer, Hiten D Madhani, Charles S Craik
The opportunistic fungal pathogen Cryptococcus neoformans is a major cause of mortality in immunocompromised individuals, resulting in more than 600,000 deaths per year. Many human fungal pathogens secrete peptidases that influence virulence, but in most cases the substrate specificity and regulation of these enzymes remains poorly understood. The paucity of such information is a roadblock to our understanding of the biological functions of peptidases and whether or not these enzymes are viable therapeutic targets...
December 2016: PLoS Pathogens
https://www.readbyqxmd.com/read/27976581/phosphatase-popx2-exhibits-dual-regulatory-functions-in-cancer-metastasis
#13
Songjing Zhang, Ting Weng, Elsie Cheruba, Tiannan Guo, Hei Chan, Siu Kwan Sze, Cheng-Gee Koh
Cancer metastasis is a complex mechanism involving multiple processes. Previously, our integrative proteome, transcriptome, and phosphoproteome study reported that the levels of serine/threonine phosphatase POPX2 were positively correlated with cancer cell motility through modulating MAPK signaling. Surprisingly, here we found that POPX2 knockdown cells induced more numerous and larger tumor nodules in lungs in longer term animal studies. Interestingly, our analysis of DNA microarray data from cancer patient samples that are available in public databases shows that low POPX2 expression is linked to distant metastasis and poor survival rate...
December 28, 2016: Journal of Proteome Research
https://www.readbyqxmd.com/read/27974393/temperature-regulated-expression-of-type-vi-secretion-systems-in-fish-pathogen-pseudomonas-plecoglossicida-revealed-by-comparative-secretome-analysis
#14
Zhen Tao, Tao Zhou, Suming Zhou, Guoliang Wang
Pseudomonas plecoglossicida is a facultative fish pathogen. Recent studies showed that P. plecoglossicida infection in fish was associated with temperature. The aim of this study was to compare the secretomes of P. plecoglossicida cultured in vitro at representative temperatures for pathogenic (20°C) and less pathogenic (30°C) phenotypes. Thirteen proteins in the culture supernatants of P. plecoglossicida showed significant difference in abundance at 20 vs. 30°C. Four proteins were strongly increased at 20°C, including two hemolysin co-regulated proteins (Hcp) that are part of the bacterial type VI secretion system (T6SS), flagellin and an unknown protein...
November 2016: FEMS Microbiology Letters
https://www.readbyqxmd.com/read/27966681/bottom-up-proteomics-analysis-of-the-secretome-of-murine-islets-of-langerhans-in-elevated-glucose-levels
#15
Andrew Schmudlach, Jeremy Felton, Robert T Kennedy, Norman J Dovichi
Glucotoxicity is a causative agent of type-2 diabetes, where high glucose levels damage the islets of Langerhans resulting in oxidative damage and endoplasmic reticulum stress. We evaluated the secretomes of healthy CD-1 murine islets. Three experimental conditions were investigated in biological triplicate: a control incubated with 11 mM glucose, 1-day incubation with 25 mM glucose, and 2-day incubation with 25 mM glucose. An SDS-based, filter-aided sample preparation protocol was used to prepare secretomes for analysis...
January 16, 2017: Analyst
https://www.readbyqxmd.com/read/27941947/bursaphelenchus-xylophilus-and-b-mucronatus-secretomes-a-comparative-proteomic-analysis
#16
Joana M S Cardoso, Sandra I Anjo, Luís Fonseca, Conceição Egas, Bruno Manadas, Isabel Abrantes
The pinewood nematode, Bursaphelenchus xylophilus, recognized as a worldwide major forest pest, is a migratory endoparasitic nematode with capacity to feed on pine tissues and also on fungi colonizing the trees. Bursaphelenchus mucronatus, the closest related species, differs from B. xylophilus on its pathogenicity, making this nematode a good candidate for comparative analyses. Secretome profiles of B. xylophilus and B. mucronatus were obtained and proteomic differences were evaluated by quantitative SWATH-MS...
December 12, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27934856/bulk-cell-density-and-wnt-tgfbeta-signalling-regulate-mesendodermal-patterning-of-human-pluripotent-stem-cells
#17
Henning Kempf, Ruth Olmer, Alexandra Haase, Annika Franke, Emiliano Bolesani, Kristin Schwanke, Diana Robles-Diaz, Michelle Coffee, Gudrun Göhring, Gerald Dräger, Oliver Pötz, Thomas Joos, Erik Martinez-Hackert, Axel Haverich, Falk F R Buettner, Ulrich Martin, Robert Zweigerdt
In vitro differentiation of human pluripotent stem cells (hPSCs) recapitulates early aspects of human embryogenesis, but the underlying processes are poorly understood and controlled. Here we show that modulating the bulk cell density (BCD: cell number per culture volume) deterministically alters anteroposterior patterning of primitive streak (PS)-like priming. The BCD in conjunction with the chemical WNT pathway activator CHIR99021 results in distinct paracrine microenvironments codifying hPSCs towards definitive endoderm, precardiac or presomitic mesoderm within the first 24 h of differentiation, respectively...
December 9, 2016: Nature Communications
https://www.readbyqxmd.com/read/27933904/global-analysis-of-secreted-proteins-and-glycoproteins-in-saccharomyces-cerevisiae
#18
Johanna M Smeekens, Haopeng Xiao, Ronghu Wu
Protein secretion is essential for numerous cellular activities, and secreted proteins in bodily fluids are a promising and noninvasive source of biomarkers for disease detection. Systematic analysis of secreted proteins and glycoproteins will provide insight into protein function and cellular activities. Yeast (Saccharomyces cerevisiae) is an excellent model system for eukaryotic cells, but global analysis of secreted proteins and glycoproteins in yeast is challenging due to the low abundances of secreted proteins and contamination from high-abundance intracellular proteins...
December 20, 2016: Journal of Proteome Research
https://www.readbyqxmd.com/read/27926865/tenascin-c-orchestrates-glioblastoma-angiogenesis-by-modulation-of-pro-and-anti-angiogenic-signaling
#19
Tristan Rupp, Benoit Langlois, Maria M Koczorowska, Agata Radwanska, Zhen Sun, Thomas Hussenet, Olivier Lefebvre, Devadarssen Murdamoothoo, Christiane Arnold, Annick Klein, Martin L Biniossek, Vincent Hyenne, Elise Naudin, Ines Velazquez-Quesada, Oliver Schilling, Ellen Van Obberghen-Schilling, Gertraud Orend
High expression of the extracellular matrix component tenascin-C in the tumor microenvironment correlates with decreased patient survival. Tenascin-C promotes cancer progression and a disrupted tumor vasculature through an unclear mechanism. Here, we examine the angiomodulatory role of tenascin-C. We find that direct contact of endothelial cells with tenascin-C disrupts actin polymerization, resulting in cytoplasmic retention of the transcriptional coactivator YAP. Tenascin-C also downregulates YAP pro-angiogenic target genes, thus reducing endothelial cell survival, proliferation, and tubulogenesis...
December 6, 2016: Cell Reports
https://www.readbyqxmd.com/read/27909049/control-of-secreted-protein-gene-expression-and-the-mammalian-secretome-by-the-metabolic-regulator-pgc-1%C3%AE
#20
Neri Minsky, Robert G Roeder
Secreted proteins serve pivotal roles in the development of multicellular organisms, acting as structural matrix, extracellular enzymes, and signal molecules. However, how the secretome is regulated remains incompletely understood. Here we demonstrate, unexpectedly, that peroxisome proliferator-activated receptor γ coactivator 1-α (PGC-1α), a critical transcriptional co-activator of metabolic gene expression, functions to down-regulate the expression of diverse genes encoding secreted molecules and extracellular matrix components to modulate the secretome...
January 6, 2017: Journal of Biological Chemistry
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