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secretome analysis

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https://www.readbyqxmd.com/read/29352743/co-transplantation-of-adipose-tissue-derived-stromal-cells-and-olfactory-ensheathing-cells-for-spinal-cord-injury-repair
#1
Eduardo D Gomes, Sofia S Mendes, Rita C Assunção-Silva, Fábio G Teixeira, Ana O Pires, Sandra I Anjo, Bruno Manadas, Hugo Leite-Almeida, Jeffrey M Gimble, Nuno Sousa, Angelo C Lepore, Nuno A Silva, António J Salgado
Patients suffering from Spinal Cord Injury (SCI) still have a dismal prognosis. Despite all the efforts developed in this area, currently there are no effective treatments. Therefore, cell therapies have been proposed as a viable alternative to the current treatments employed. Adipose tissue-derived Stem Cells (ASCs) and Olfactory Ensheathing Cells (OECs) have been used with promising results in different models of SCI, namely due to the regenerative properties of the secretome of the first, and the guidance capability of the second...
January 20, 2018: Stem Cells
https://www.readbyqxmd.com/read/29348510/genome-and-secretome-analysis-of-pochonia-chlamydosporia-provide-new-insight-into-egg-parasitic-mechanisms
#2
Runmao Lin, Feifei Qin, Baoming Shen, Qianqian Shi, Chichuan Liu, Xi Zhang, Yang Jiao, Jun Lu, Yaoyao Gao, Marta Suarez-Fernandez, Federico Lopez-Moya, Luis Vicente Lopez-Llorca, Gang Wang, Zhenchuan Mao, Jian Ling, Yuhong Yang, Xinyue Cheng, Bingyan Xie
Pochonia chlamydosporia infects eggs and females of economically important plant-parasitic nematodes. The fungal isolates parasitizing different nematodes are genetically distinct. To understand their intraspecific genetic differentiation, parasitic mechanisms, and adaptive evolution, we assembled seven putative chromosomes of P. chlamydosporia strain 170 isolated from root-knot nematode eggs (~44 Mb, including 7.19% of transposable elements) and compared them with the genome of the strain 123 (~41 Mb) isolated from cereal cyst nematode...
January 18, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29343217/ceratocystis-cacaofunesta-genome-analysis-reveals-a-large-expansion-of-extracellular-phosphatidylinositol-specific-phospholipase-c-genes-pi-plc
#3
Eddy Patricia Lopez Molano, Odalys García Cabrera, Juliana Jose, Leandro Costa do Nascimento, Marcelo Falsarella Carazzolle, Paulo José Pereira Lima Teixeira, Javier Correa Alvarez, Ricardo Augusto Tiburcio, Paulo Massanari Tokimatu Filho, Gustavo Machado Alvares de Lima, Rafael Victório Carvalho Guido, Thamy Lívia Ribeiro Corrêa, Adriana Franco Paes Leme, Piotr Mieczkowski, Gonçalo Amarante Guimarães Pereira
BACKGROUND: The Ceratocystis genus harbors a large number of phytopathogenic fungi that cause xylem parenchyma degradation and vascular destruction on a broad range of economically important plants. Ceratocystis cacaofunesta is a necrotrophic fungus responsible for lethal wilt disease in cacao. The aim of this work is to analyze the genome of C. cacaofunesta through a comparative approach with genomes of other Sordariomycetes in order to better understand the molecular basis of pathogenicity in the Ceratocystis genus...
January 17, 2018: BMC Genomics
https://www.readbyqxmd.com/read/29340032/c3g-promotes-a-selective-release-of-angiogenic-factors-from-activated-mouse-platelets-to-regulate-angiogenesis-and-tumor-metastasis
#4
Víctor Martín-Granado, Sara Ortiz-Rivero, Rita Carmona, Sara Gutiérrez-Herrero, Mario Barrera, Laura San-Segundo, Celia Sequera, Pedro Perdiguero, Francisco Lozano, Francisco Martín-Herrero, José Ramón González-Porras, Ramón Muñoz-Chápuli, Almudena Porras, Carmen Guerrero
Previous observations indicated that C3G (RAPGEF1) promotes α-granule release, evidenced by the increase in P-selectin exposure on the platelet surface following its activation. The goal of the present study is to further characterize the potential function of C3G as a modulator of the platelet releasate and its implication in the regulation of angiogenesis. Proteomic analysis revealed a decreased secretion of anti-angiogenic factors from activated transgenic C3G and C3G∆Cat platelets. Accordingly, the secretome from both transgenic platelets had an overall pro-angiogenic effect as evidenced by an in vitro capillary-tube formation assay with HUVECs (human umbilical vein endothelial cells) and by two in vivo models of heterotopic tumor growth...
December 19, 2017: Oncotarget
https://www.readbyqxmd.com/read/29322779/mining-the-secretome-of-c2c12-muscle-cells-data-dependent-experimental-approach-to-analyze-protein-secretion-using-label-free-quantification-and-peptide-based-analysis
#5
Leonie Grube, Rafael Dellen, Fabian Kruse, Holger Schwender, Kai Stuehler, Gereon Poschmann
Secretome analysis faces several challenges, including detection of low abundant proteins and the discrimination of bona fide secreted proteins from false-positive identifications stemming from cell leakage or serum. Here, we developed a two-step secretomics approach and applied it to the analysis of secreted proteins of C2C12 skeletal muscle cells since the skeletal muscle has been identified as an important endocrine organ secreting myokines as signaling molecules. First, we compared culture supernatants with corresponding cell lysates by mass spectrometry-based proteomics and label-free quantification...
January 11, 2018: Journal of Proteome Research
https://www.readbyqxmd.com/read/29322262/partial-secretome-analysis-of-caldariomyces-fumago-reveals-extracellular-production-of-the-cpo-co-substrate-h2o2-and-provides-a-coproduction-concept-for-cpo-and-glucose-oxidase
#6
Markus Buchhaupt, Karin Lintz, Sonja Hüttmann, Jens Schrader
The culture supernatant of Caldariomyces fumago strains grown in a minimal medium with fructose contains mainly the biotechnologically relevant enzyme chloroperoxidase (CPO) and only minor amounts of other proteins. Our approach to identify the nature of these proteins via peptide mass fingerprinting and transcriptome analysis demonstrated the presence of putative glycosyl hydrolase and glucose oxidase (GOx) enzymes. These activities had been described earlier as parts of the fungus´ halogenation machinery, as they provide CPO with the co-substrate H2O2...
January 10, 2018: World Journal of Microbiology & Biotechnology
https://www.readbyqxmd.com/read/29321187/infection-by-the-helminth-parasite-fasciola-hepatica-requires-rapid-regulation-of-metabolic-virulence-and-invasive-factors-to-adjust-to-its-mammalian-host
#7
Krystyna Cwiklinski, Heather Jewhurst, Paul McVeigh, Tara Barbour, Aaron G Maule, Jose Tort, Sandra M O'Neill, Mark W Robinson, Sheila Donnelly, John P Dalton
The parasite Fasciola hepatica infects a broad range of mammals with impunity.  Following ingestion of parasites (metacercariae) by the host, newly excysted juveniles (NEJ) emerge from their cysts, rapidly penetrate the duodenal wall and migrate to the liver.  Successful infection takes just a few hours and involves negotiating hurdles presented by host macromolecules, tissues and micro-environments, as well as the immune system.  Here, transcriptome and proteome analysis of ex vivo F. hepatica metacercariae and NEJ reveal the rapidity and multitude of metabolic and developmental alterations that take place in order for the parasite to establish infection...
January 10, 2018: Molecular & Cellular Proteomics: MCP
https://www.readbyqxmd.com/read/29297499/differential-regulation-of-the-transcriptomic-and-secretomic-landscape-of-sensor-and-effector-functions-of-human-airway-epithelial-cells
#8
Roland Lehmann, Mario M Müller, Tilman E Klassert, Dominik Driesch, Magdalena Stock, Anina Heinrich, Theresia Conrad, Christoph Moore, Uta K Schier, Reinhard Guthke, Hortense Slevogt
Protein secretion upon TLR, TNFR1, and IFNGR ligation in the human airways is considered to be central for the orchestration of pulmonary inflammatory and immune responses. In this study, we compared the gene expression and protein secretion profiles in response to specific stimulation of all expressed TLRs and in further comparison to TNFR1 and IFNGR in primary human airway epithelial cells. In addition to 22 cytokines, we observed the receptor-induced regulation of 571 genes and 1,012 secreted proteins. Further analysis revealed high similarities between the transcriptional TLR sensor and TNFR1 effector responses...
January 3, 2018: Mucosal Immunology
https://www.readbyqxmd.com/read/29290811/development-and-comparison-of-two-immuno-disaggregation-based-bioassays-for-cell-secretome-analysis
#9
Pawan Kc, Fan Liu, Jiang Zhe, Ge Zhang
Cell secretome analysis has gained increasing attention towards the development of effective strategies for disease treatment. Analysis of cell secretome enables the platform to monitor the status of disease progression, facilitating therapeutic outcomes. However, cell secretome analysis is very challenging due to its versatile and dynamic composition. Here, we report the development of two immuno-disaggregation bioassays using functionalized microparticles for the quantitative analysis of the cell secretome...
2018: Theranostics
https://www.readbyqxmd.com/read/29290529/histone-deacetylase-inhibitor-hdaci-upregulates-activin-a-and-activates-the-smad-signaling-pathway-in-melanomas
#10
Jeeyong Lee, Jiwon Ko, Jae Youn Yi
BACKGROUND: Histone deacetylase (HDAC) is an enzyme that regulates gene expression, cell cycle arrest, apoptosis and modulation of various pathways. HDAC inhibitors (HDACis) can modulate these pathways by hyper-acetylating target proteins, thereby acting as cancer chemotherapeutic agents. OBJECTIVE: One of HDACis, suberoylanilide hydroxamic acid (SAHA), has been found to regulate the Smad signaling pathway, by an as yet unclear mechanism. This study therefore investigated the mechanism by which SAHA regulates Smad signaling in the melanoma cell line SK-Mel-5...
December 21, 2017: Journal of Dermatological Science
https://www.readbyqxmd.com/read/29285222/a-primary-tumor-gene-expression-signature-identifies-a-crucial-role-played-by-tumor-stroma-myofibroblasts-in-lymph-node-involvement-in-oral-squamous-cell-carcinoma
#11
Gianluigi Mazzoccoli, Stefano Castellana, Massimo Carella, Orazio Palumbo, Cristiana Tiberio, Caterina Fusilli, Daniele Capocefalo, Tommaso Biagini, Tommaso Mazza, Lorenzo Lo Muzio
Oral squamous cell carcinoma (OSCC) is the most common oral and pharyngeal cancer, and is responsible of approximately 3% of cancers in men and 2% in women in the Western World, with increasing incidence rates in developing countries. Early detection by screening is necessary to prevent fatal disease because early, curable lesions are rarely symptomatic. The overall 5-yr survival rate is approximately 50% when surgery, radiation, or both are employed as treatment options, but lymph node involvement greatly influences this estimate, by decreasing the survival rate by about 50%...
December 1, 2017: Oncotarget
https://www.readbyqxmd.com/read/29275043/signatures-of-protein-expression-revealed-by-secretome-analyses-of-cancer-associated-fibroblasts-and-melanoma-cell-lines
#12
Tarcísio Liberato, Dayelle S Pessotti, Isabella Fukushima, Eduardo S Kitano, Solange M T Serrano, André Zelanis
The imbalance of cellular homeostasis during oncogenesis together with the high heterogeneity of tumor-associated stromal cells have a marked effect on the repertoire of the proteins secreted by malignant cells (the secretome). Hence, the study of tumoral secretomes provides insights for understanding the cross-talk between cells within the tumor microenvironment as well as the key effectors for the establishment of the pre-metastatic niche in distant tumor sites. In this context, we performed a proteomic analysis of the secretomes derived from four cell lines: a paired set of fibroblasts - Hs 895...
December 21, 2017: Journal of Proteomics
https://www.readbyqxmd.com/read/29247453/plasma-adrenocorticotropic-hormone-acth-concentrations-in-ponies-measured-by-two-different-assays-suggests-seasonal-cross-reactivity-or-interference
#13
E J Knowles, M C Moreton-Clack, S Shaw, P A Harris, J Elliott, N J Menzies-Gow
BACKGROUND: Analysis of plasma adrenocorticotropic hormone concentration [ACTH] aids diagnosis of pituitary pars intermedia dysfunction (PPID). Comparisons of validated chemiluminescent-immunoassay (CI) and immunofluorescent (IF) assays are limited. OBJECTIVES: To compare the results of [ACTH] analysis by CI and IF methods of samples collected in autumn and spring and assess cross-reactivity. STUDY DESIGN: Method comparison. METHODS: Plasma from non-laminitic ponies was analysed concurrently using the IF and CI methods in autumn and the following spring...
December 16, 2017: Equine Veterinary Journal
https://www.readbyqxmd.com/read/29238343/thrombospondin-1-partly-mediates-the-cartilage-protective-effect-of-adipose-derived-mesenchymal-stem-cells-in-osteoarthritis
#14
Marie Maumus, Cristina Manferdini, Karine Toupet, Paul Chuchana, Louis Casteilla, Mélanie Gachet, Christian Jorgensen, Gina Lisignoli, Danièle Noël
Objective: Assuming that mesenchymal stem cells (MSCs) respond to the osteoarthritic joint environment to exert a chondroprotective effect, we aimed at investigating the molecular response setup by MSCs after priming by osteoarthritic chondrocytes in cocultures. Methods: We used primary human osteoarthritic chondrocytes and adipose stem cells (ASCs) in mono- and cocultures and performed a high-throughput secretome analysis. Among secreted proteins differentially induced in cocultures, we identified thrombospondin-1 (THBS1) as a potential candidate that could be involved in the chondroprotective effect of ASCs...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/29236046/advances-in-proteomic-techniques-for-cytokine-analysis-focus-on-melanoma-research
#15
REVIEW
Helena Kupcova Skalnikova, Jana Cizkova, Jakub Cervenka, Petr Vodicka
Melanoma is a skin cancer with permanently increasing incidence and resistance to therapies in advanced stages. Reports of spontaneous regression and tumour infiltration with T-lymphocytes makes melanoma candidate for immunotherapies. Cytokines are key factors regulating immune response and intercellular communication in tumour microenvironment. Cytokines may be used in therapy of melanoma to modulate immune response. Cytokines also possess diagnostic and prognostic potential and cytokine production may reflect effects of immunotherapies...
December 13, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29233912/dynamic-secretome-of-trichomonas-vaginalis-case-study-of-%C3%AE-amylases
#16
Jitka Štáfková, Petr Rada, Dionigia Meloni, Vojtěch Žárský, Tamara Smutná, Nadine Zimmann, Karel Harant, Petr Pompach, Ivan Hrdý, Jan Tachezy
The secretion of virulence factors by parasitic protists into the host environment plays a fundamental role in multifactorial host-parasite interactions. Several effector proteins are known to be secreted by Trichomonas vaginalis, a human parasite of the urogenital tract. However, a comprehensive profiling of the T. vaginalis secretome remains elusive, as do the mechanisms of protein secretion. In this study, we used high-resolution label-free quantitative MS to analyze the T. vaginalis secretome, considering that secretion is a time- and temperature-dependent process, to define the cutoff for secreted proteins...
December 12, 2017: Molecular & Cellular Proteomics: MCP
https://www.readbyqxmd.com/read/29230233/whole-genome-sequencing-of-fusarium-fujikuroi-provides-insight-into-the-role-of-secretory-proteins-and-cell-wall-degrading-enzymes-in-causing-bakanae-disease-of-rice
#17
Bishnu M Bashyal, Kirti Rawat, Sapna Sharma, Deepika Kulshreshtha, S Gopala Krishnan, Ashok K Singh, Himanshu Dubey, Amolkumar U Solanke, T R Sharma, Rashmi Aggarwal
Fusarium fujikuroi causing bakanae disease has emerged as one of the major pathogen of rice across the world. The study aims to comparative genomic analysis of Fusarium fujikuroi isolates and identification of the secretary proteins of the fungus involved in rice pathogenesis. In the present study, F. fujikuroi isolate "F250" was sequenced with an assembly size of 42.47 Mb providing coverage of 96.89% on reference IMI58289 genome. A total of 13,603 protein-coding genes were predicted from genome assembly. The average gene density in the F...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/29216817/characterization-of-sulfhydryl-oxidase-from-aspergillus-tubingensis
#18
Outi Nivala, Greta Faccio, Mikko Arvas, Perttu Permi, Johanna Buchert, Kristiina Kruus, Maija-Liisa Mattinen
BACKGROUND: Despite of the presence of sulfhydryl oxidases (SOXs) in the secretomes of industrially relevant organisms and their many potential applications, only few of these enzymes have been biochemically characterized. In addition, basic functions of most of the SOX enzymes reported so far are not fully understood. In particular, the physiological role of secreted fungal SOXs is unclear. RESULTS: The recently identified SOX from Aspergillus tubingensis (AtSOX) was produced, purified and characterized in the present work...
December 8, 2017: BMC Biochemistry
https://www.readbyqxmd.com/read/29213115/modulation-of-immune-signaling-and-metabolism-highlights-host-and-fungal-transcriptional-responses-in-mouse-models-of-invasive-pulmonary-aspergillosis
#19
Shiv D Kale, Tariq Ayubi, Dawoon Chung, Nuria Tubau-Juni, Andrew Leber, Ha X Dang, Saikumar Karyala, Raquel Hontecillas, Christopher B Lawrence, Robert A Cramer, Josep Bassaganya-Riera
Incidences of invasive pulmonary aspergillosis, an infection caused predominantly by Aspergillus fumigatus, have increased due to the growing number of immunocompromised individuals. While A. fumigatus is reliant upon deficiencies in the host to facilitate invasive disease, the distinct mechanisms that govern the host-pathogen interaction remain enigmatic, particularly in the context of distinct immune modulating therapies. To gain insights into these mechanisms, RNA-Seq technology was utilized to sequence RNA derived from lungs of 2 clinically relevant, but immunologically distinct murine models of IPA on days 2 and 3 post inoculation when infection is established and active disease present...
December 6, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29204926/alginate-liposomal-construct-for-bupivacaine-delivery-and-msc-function-regulation
#20
Mollie S Davis, Ileana Marrero-Berrios, Xiomara I Perez, Timothy Maguire, Palangat Radhakrishnan, Devasena Manchikalapati, Joseph SchianodiCola, Hattiyangangadi Kamath, Rene S Schloss, Joel Yarmush
Mesenchymal stromal cell (MSC) therapies have become potential treatment options for multiple ailments and traumatic injuries. In the clinical setting, MSC are likely to be co-administered with local anesthetics (LA) which have been shown to have dose- and potency-dependent detrimental effects on the viability and function of cells. We previously developed and characterized a sustained-release LA delivery formulation comprised of alginate-encapsulated liposomal bupivacaine. The current studies were designed to evaluate the effect of this formulation on the secretion of three key MSC regulatory molecules, interleukin 6 (IL-6), prostaglandin E2 (PGE2), and transforming growth factor-beta 1 (TGF-β1)...
December 4, 2017: Drug Delivery and Translational Research
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