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https://www.readbyqxmd.com/read/28931551/endoplasmic-reticulum-membrane-protein-complex-subunit-10-emc10-is-a-bone-marrow-derived-angiogenic-growth-factor-promoting-tissue-repair-after-myocardial-infarction
#1
Marc R Reboll, Mortimer Korf-Klingebiel, Stefanie Klede, Felix Polten, Eva Brinkmann, Ines Reimann, Hans-Joachim Schönfeld, Maria Bobadilla, Jan Faix, George Kensah, Ina Gruh, Michael Klintschar, Matthias Gaestel, Hans W Niessen, Andreas Pich, Johann Bauersachs, Joseph A Gogos, Yong Wang, Kai C Wollert
Background -Clinical trials of bone marrow cell (BMC)-based therapies after acute myocardial infarction (MI) have produced mostly neutral results. Treatment with specific BMC-derived secreted proteins may provide an alternative biologic approach to improving tissue repair and heart function after MI. We recently performed a bioinformatic secretome analysis in BMCs from patients with acute MI and discovered a poorly characterized secreted protein, endoplasmic reticulum membrane protein complex subunit 10 (EMC10), showing activity in an angiogenic screen...
September 20, 2017: Circulation
https://www.readbyqxmd.com/read/28919884/secretome-of-intestinal-bacilli-a-natural-guard-against-pathologies
#2
REVIEW
Olga N Ilinskaya, Vera V Ulyanova, Dina R Yarullina, Ilgiz G Gataullin
Current studies of human gut microbiome usually do not consider the special functional role of transient microbiota, although some of its members remain in the host for a long time and produce broad spectrum of biologically active substances. Getting into the gastrointestinal tract (GIT) with food, water and probiotic preparations, two representatives of Bacilli class, genera Bacillus and Lactobacillus, colonize epithelium blurring the boundaries between resident and transient microbiota. Despite their minor proportion in the microbiome composition, these bacteria can significantly affect both the intestinal microbiota and the entire body thanks to a wide range of secreted compounds...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28903359/expression-of-sialyl-tn-sugar-antigen-in-bladder-cancer-cells-affects-response-to-bacillus-calmette-gu%C3%A3-rin-bcg-and-to-oxidative-damage
#3
Paulo F Severino, Mariana Silva, Mylene Carrascal, Nadia Malagolini, Mariella Chiricolo, Giulia Venturi, Annalisa Astolfi, Mariangela Catera, Paula A Videira, Fabio Dall'Olio
The sialyl-Tn (sTn) antigen is an O-linked carbohydrate chain aberrantly expressed in bladder cancer (BC), whose biosynthesis is mainly controlled by the sialyltransferase ST6GALNAC1. Treatment with Bacillus Calmette-Guérin (BCG) is the most effective adjuvant immunotherapy for superficial BC but one third of the patients fail to respond. A poorly understood correlation between the expression of sTn and BC patient's response to BCG was previously observed. By analyzing tumor tissues, we showed that patients with high ST6GALNAC1 and IL-6 mRNA expression were BCG responders...
August 15, 2017: Oncotarget
https://www.readbyqxmd.com/read/28898534/proteomic-analysis-of-the-secretome-of-porcine-alveolar-macrophages-infected-with-porcine-reproductive-and-respiratory-syndrome-virus
#4
Yuming Li, Zhuanchang Wu, Ke Liu, Pengfei Qi, Jinpeng Xu, Jianchao Wei, Beibei Li, Donghua Shao, Yuanyuan Shi, Yafeng Qiu, Zhiyong Ma
Porcine reproductive and respiratory syndrome virus (PRRSV) causes porcine reproductive and respiratory syndrome, which is characterized by reproductive failure and respiratory disorders. The secretome of PRRSV-infected porcine alveolar macrophages (PAMs), which are the primary target cells of PRRSV, was analyzed by label-free quantitative proteomics to gain a profile of proteins secreted during PRRSV infection. A total of 95 secreted proteins with differentially expressed levels between PRRSV- and mock-infected PAMs was screened...
September 12, 2017: Proteomics
https://www.readbyqxmd.com/read/28886382/distinct-mesenchymal-lineages-and-niches-promote-epithelial-self-renewal-and-myofibrogenesis-in-the-lung
#5
Jarod A Zepp, William J Zacharias, David B Frank, Christina A Cavanaugh, Su Zhou, Michael P Morley, Edward E Morrisey
The lung is an architecturally complex organ comprising a heterogeneous mixture of various epithelial and mesenchymal lineages. We use single-cell RNA sequencing and signaling lineage reporters to generate a spatial and transcriptional map of the lung mesenchyme. We find that each mesenchymal lineage has a distinct spatial address and transcriptional profile leading to unique niche regulatory functions. The mesenchymal alveolar niche cell is Wnt responsive, expresses Pdgfrα, and is critical for alveolar epithelial cell growth and self-renewal...
September 7, 2017: Cell
https://www.readbyqxmd.com/read/28874693/comparative-secretome-analysis-of-rhizoctonia-solani-isolates-with-different-host-ranges-reveals-unique-secretomes-and-cell-death-inducing-effectors
#6
Jonathan P Anderson, Jana Sperschneider, Joe Win, Brendan Kidd, Kentaro Yoshida, James Hane, Diane G O Saunders, Karam B Singh
Rhizoctonia solani is a fungal pathogen causing substantial damage to many of the worlds' largest food crops including wheat, rice, maize and soybean. Despite impacting global food security, little is known about the pathogenicity mechanisms employed by R. solani. To enable prediction of effectors possessing either broad efficacy or host specificity, a combined secretome was constructed from a monocot specific isolate, a dicot specific isolate and broad host range isolate infecting both monocot and dicot hosts...
September 5, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28861815/bioinformatics-analysis-of-protein-secretion-in-plants
#7
Liyuan Chen
In sessile plants, the dynamic protein secretion pathways orchestrate the cellular responses to internal signals and external environmental changes in almost every aspect of plant developmental events. The cohort of plant proteins, secreted from the plant cells into the extracellular matrix, has been annotated as plant secretome. Therefore, the identification and characterization of secreted proteins will discover novel secretory potentials and establish the functional connection between cellular protein secretion and plant physiological phenomena...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28845240/human-protein-secretory-pathway-genes-are-expressed-in-a-tissue-specific-pattern-to-match-processing-demands-of-the-secretome
#8
Amir Feizi, Francesco Gatto, Mathias Uhlen, Jens Nielsen
Protein secretory pathway in eukaryal cells is responsible for delivering functional secretory proteins. The dysfunction of this pathway causes a range of important human diseases from congenital disorders to cancer. Despite the piled-up knowledge on the molecular biology and biochemistry level, the tissue-specific expression of the secretory pathway genes has not been analyzed on the transcriptome level. Based on the recent RNA-sequencing studies, the largest fraction of tissue-specific transcriptome encodes for the secretome (secretory proteins)...
2017: NPJ Systems Biology and Applications
https://www.readbyqxmd.com/read/28842514/impaired-innate-immune-gene-profiling-in-airway-smooth-muscle-cells-from-chronic-cough-patients
#9
Christos Rossios, Stelios Pavlidis, David Gibeon, Sharon Mumby, Andrew Durham, Oluwaseun Ojo, Daniel Horowitz, Matt Loza, Fred Baribaud, Navin Rao, Kian Fan Chung, Ian M Adcock
Chronic cough is associated with airway inflammation and remodelling. Abnormal airway smooth muscle cell (ASMC) function may underlie mechanisms of chronic cough.  Our objective was to examine the transcriptome and focused secretome of ASMCs from chronic cough patients and healthy non-cough volunteers.  ASMC gene expression profiling was performed at baseline and/or after stimulation with polyinosinic:polycytidylic acid (poly(I:C)) to mimic viral infection.  Supernatants were collected for multiplex analysis...
August 25, 2017: Bioscience Reports
https://www.readbyqxmd.com/read/28837649/integrative-analysis-for-the-discovery-of-lung-cancer-serological-markers-and-validation-by-mrm-ms
#10
Jihye Shin, Sang-Yun Song, Hee-Sung Ahn, Byung Chull An, Yoo-Duk Choi, Eun Gyeong Yang, Kook-Joo Na, Seung-Taek Lee, Jae-Il Park, Seon-Young Kim, Cheolju Lee, Seung-Won Lee
Non-small-cell lung cancer (NSCLC) constitutes approximately 80% of all diagnosed lung cancers, and diagnostic markers detectable in the plasma/serum of NSCLC patients are greatly needed. In this study, we established a pipeline for the discovery of markers using 9 transcriptome datasets from publicly available databases and profiling of six lung cancer cell secretomes. Thirty-one out of 312 proteins that overlapped between two-fold differentially expressed genes and identified cell secretome proteins were detected in the pooled plasma of lung cancer patients...
2017: PloS One
https://www.readbyqxmd.com/read/28835855/chemotherapeutic-xct-inhibitors-sorafenib-and-erastin-unraveled-with-the-synaptic-optogenetic-function-analysis-tool
#11
Marc Dahlmanns, Eduard Yakubov, Daishi Chen, Tina Sehm, Manfred Rauh, Nicolai Savaskan, Jana Katharina Wrosch
In the search for new potential chemotherapeutics, the compounds' toxicity to healthy cells is an important factor. The brain with its functional units, the neurons, is especially endangered during the radio- and chemotherapeutic treatment of brain tumors. The effect of the potential compounds not only on neuronal survival but also neuronal function needs to be taken into account. Therefore, in this study we aimed to comprehend the biological effects of chemotherapeutic xCT inhibition on healthy neuronal cells with our synaptic optogenetic function analysis tool (SOFA)...
2017: Cell Death Discovery
https://www.readbyqxmd.com/read/28831735/identification-heterologous-expression-and-characterization-of-a-dye-decolorizing-peroxidase-of-pleurotus-sapidus
#12
Christiane Lauber, Tatiana Schwarz, Quoc Khanh Nguyen, Patrick Lorenz, Guenter Lochnit, Holger Zorn
The coding sequence of a peroxidase from the secretome of Pleurotus sapidus was cloned from a cDNA library. Bioinformatic analyses revealed an open reading frame of 1551 bp corresponding to a primary translation product of 516 amino acids. The DyP-type peroxidase was heterologously produced in Trichoderma reesei with an activity of 55,000 U L(-1). The enzyme was purified from the culture supernatant, biochemically characterized and the kinetic parameters were determined. The enzyme has an N-terminal signal peptide composed of 62 amino acids...
August 23, 2017: AMB Express
https://www.readbyqxmd.com/read/28829806/genome-engineering-in-bacillus-anthracis-using-tyrosine-site-specific-recombinases
#13
Andrei P Pomerantsev, Rita M McCall, Margaret Chahoud, Nathan K Hepler, Rasem Fattah, Stephen H Leppla
Tyrosine site-specific recombinases (T-SSR) are polynucleotidyltransferases that catalyze cutting and joining reactions between short specific DNA sequences. We developed three systems for performing genetic modifications in Bacillus anthracis that use T-SSR and their cognate target sequences, namely Escherichia coli bacteriophage P1 Cre-loxP, Saccharomyces cerevisiae Flp-FRT, and a newly discovered IntXO-PSL system from B. anthracis plasmid pXO1. All three tyrosine recombinase systems were used for creation of a B...
2017: PloS One
https://www.readbyqxmd.com/read/28827399/identification-of-nidogen-1-as-a-lung-metastasis-protein-through-secretome-analysis
#14
Maša Alečković, Yong Wei, Gary LeRoy, Simone Sidoli, Daniel D Liu, Benjamin A Garcia, Yibin Kang
Secreted proteins play crucial roles in mediating tumor-stroma interactions during metastasis of cancer to different target organs. To comprehensively profile secreted proteins involved in lung metastasis, we applied quantitative mass spectrometry-based proteomics and identified 392 breast cancer-derived and 302 melanoma-derived proteins secreted from highly lung metastatic cells. The cancer-specific lung metastasis secretome signatures (LMSSs) displayed significant prognostic value in multiple cancer clinical data sets...
July 15, 2017: Genes & Development
https://www.readbyqxmd.com/read/28827309/withdrawn-identification-of-an-endogenous-redox-partner-for-lytic-polysaccharide-monooxygenase-based-oxidative-cleavage-of-polysaccharides
#15
Nidhi Adlakha, Girish H Rajacharya, Rakesh Bhatnagar
This article has been withdrawn by the authors. Figs 4B and 6C were inappropriately presented.
August 21, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28813021/enhanced-cell-growth-of-adipocyte-derived-mesenchymal-stem-cells-using-chemically-defined-serum-free-media
#16
Myung-Suk Lee, Christine Youn, Jeong Hyun Kim, Byoung Jun Park, Jongchan Ahn, Sungyoul Hong, Young-Deug Kim, Young Kee Shin, Sang Gyu Park
The multipotency and anti-inflammatory effects of mesenchymal stem cells (MSCs) make them attractive for cell therapy in regenerative medicine. A large number of MSCs is required for efficient therapy owing to the low homing efficiency of MSCs to target sites. Furthermore, owing to limitations in obtaining sufficient amounts of MSCs, in vitro expansion of MSCs that preserves their differentiation and proliferative potential is essential. The animal factor included in culture media also limits clinical application...
August 16, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28807829/the-magnaporthe-oryzae-alt-a-1-like-protein-mohrip1-binds-to-the-plant-plasma-membrane
#17
Yi Zhang, Yingbo Liang, Yijie Dong, Yuhan Gao, Xiufen Yang, Jingjing Yuan, Dewen Qiu
MoHrip1, a protein isolated from Magnaporthe oryzae, belongs to the Alt A 1 (AA1) family. mohrip1 mRNA levels showed inducible expression throughout the infection process in rice. To determine the location of MoHrip1 in M. oryzae, a mohrip1-gfp mutant was generated. Fluorescence microscopy observations and western blotting analysis showed that MoHrip1 was both present in the secretome and abundant in the fungal cell wall. To obtain MoHrip1 protein, we carried out high-yield expression of MoHrip1 in Pichia pastoris...
August 12, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28805233/analytic-and-dynamic-secretory-profile-of-patient-derived-cytokine-induced-killer-cells
#18
Giulia Mesiano, Roberta Zini, Giulia Montagner, Nicoletta Bianchi, Rossella Manfredini, Antonella Chillemi, Massimo Aglietta, Giovanni Grignani, Ilaria Lampronti, Erika Fiorino, Fabio Malavasi, Dario Sangiolo, Roberto Gambari, Davide Ferrari
Adoptive immunotherapy with Cytokine Induced Killer (CIK) cells has shown antitumor activity against several kinds of cancers in preclinical models and clinical trials. CIK cells are a subset of ex vivo expanded T lymphocytes with T-NK phenotype and MHC-unrestricted antitumor activity. Literature provides scanty information on cytokines, chemokines and growth factors secreted by CIK cells. Therefore, we investigated the secretory profile of CIK cells generated from tumor patients. The secretome analysis was performed at specific time points (day 1, day 14 and day 21) of CIK cells expansion...
August 9, 2017: Molecular Medicine
https://www.readbyqxmd.com/read/28782881/from-peptide-masses-to-pregnancy-maintenance-a-comprehensive-proteomic-analysis-of-the-early-equine-embryo-secretome-blastocoel-fluid-and-capsule
#19
Aleona Swegen, Christopher Gerald Grupen, Zamira Gibb, Mark Baker, Marta de Ruijter-Villani, Nathan Druery Smith, Tom A E Stout, R John Aitken
Early pregnancy in the mare is a poorly understood, high risk period during which the embryo communicates its presence to the maternal endometrium. Remarkably, the maternal recognition of pregnancy signal is unknown in the horse. This study aimed to profile the proteins secreted by equine blastocysts into their immediate environment, along with proteins contained in the blastocoel and within the acellular embryo capsule. Embryos were recovered on day 8 after ovulation and cultured for 48 hours. Secretomes of day 9 and day 10 embryos were analyzed by LC-MS/MS and supported by analysis of blastocoel fluid and embryo capsule...
August 7, 2017: Proteomics
https://www.readbyqxmd.com/read/28777000/quantitative-proteomics-reveals-temporal-proteomic-changes-in-signaling-pathways-during-bv2-mouse-microglial-cell-activation
#20
Jongmin Woo, Dohyun Han, Joseph Injae Wang, Joonho Park, Hyunsoo Kim, Youngsoo Kim
The development of systematic proteomic quantification techniques in systems biology research has enabled one to perform an in-depth analysis of cellular systems. We have developed a systematic proteomic approach that encompasses the spectrum from global to targeted analysis on a single platform. We have applied this technique to an activated microglia cell system to examine changes in the intracellular and extracellular proteomes. Microglia become activated when their homeostatic microenvironment is disrupted...
August 18, 2017: Journal of Proteome Research
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