Hongxia Yan, Abdullah Ali, Lionel Blanc, Anupama Narla, Joseph M Lane, Erjing Gao, Julien Papoin, John Hale, Christopher D Hillyer, Naomi Taylor, Patrick G Gallagher, Azra Raza, Sandrina Kinet, Narla Mohandas
Identification of stage-specific erythroid cells is critical for studies of normal and disordered human erythropoiesis. While immunophenotypic strategies have previously been developed to identify cells at each stage of terminal erythroid differentiation, erythroid progenitors are currently defined very broadly. Refined strategies to identify and characterize BFU-E and CFU-E subsets are critically needed. To address this unmet need, a flow cytometry-based technique was developed that combines the established surface markers CD34 and CD36 with CD117, CD71, and CD105...
September 1, 2021: American Journal of Hematology