keyword
MENU ▼
Read by QxMD icon Read
search

3' utr

keyword
https://www.readbyqxmd.com/read/28102759/antisense-transcription-of-the-myotonic-dystrophy-locus-yields-low-abundant-rnas-with-and-without-cag-n-repeat
#1
Anke E E G Gudde, Simon J van Heeringen, Amanda I de Oude, Ingeborg D G van Kessel, Joseph Estabrook, Eric T Wang, Bé Wieringa, Derick G Wansink
The unstable (CTG·CAG)n trinucleotide repeat in the myotonic dystrophy type 1 (DM1) locus is bidirectionally transcribed from genes with terminal overlap. By transcription in the sense direction, the DMPK gene produces various alternatively spliced mRNAs with a (CUG)n repeat in their 3' UTR. Expression in opposite orientation reportedly yields (CAG)n-repeat containing RNA, but both structure and biological significance of this antisense gene (DM1-AS) are largely unknown. Via a combinatorial approach of computational and experimental analyses of RNA from unaffected individuals and DM1 patients we discovered that DM1-AS spans >6 kb, contains alternative transcription start sites and uses alternative polyadenylation sites up- and downstream of the (CAG)n repeat...
January 19, 2017: RNA Biology
https://www.readbyqxmd.com/read/28101524/the-role-of-dopamine-in-anticipatory-pursuit-eye-movements-insights-from-genetic-polymorphisms-in-healthy-adults
#2
Jutta Billino, Jürgen Hennig, Karl R Gegenfurtner
There is a long history of eye movement research in patients with psychiatric diseases for which dysfunctions of neurotransmission are considered to be the major pathologic mechanism. However, neuromodulation of oculomotor control is still hardly understood. We aimed to investigate in particular the impact of dopamine on smooth pursuit eye movements. Systematic variability in dopaminergic transmission due to genetic polymorphisms in healthy subjects offers a noninvasive opportunity to determine functional associations...
November 2016: ENeuro
https://www.readbyqxmd.com/read/28101191/microrna-212-suppresses-the-proliferation-and-migration-of-osteosarcoma-cells-by-targeting-forkhead-box-protein-a1
#3
Jian Liu, Bohua Chen, Bin Yue, Junde Yang
MicroRNAs (miRNAs) are a class of small non-coding RNAs that function as critical gene regulators by targeting the 3' untranslated region (UTR) of mRNA, causing translational repression or mRNA degradation. Deregulation of specific miRNAs, including miR-212, has been identified in patients with osteosarcoma. However, the underlying mechanism is yet to be fully elucidated. The present study aimed to reveal the regulatory mechanism of miR-212 in osteosarcoma cell viability and migration. Quantitative polymerase chain reaction data revealed that miR-212 was significantly downregulated in osteosarcoma tissues compared with normal bone tissues...
December 2016: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/28100304/iron-responsive-like-elements-in-the-parasite-entamoeba-histolytica
#4
Liliana Soto-Castro, Laura Yuliana Plata-Guzman, Elisa Elvira Figueroa-Angulo, Jaeson Calla-Choque, Magda Reyes-Lopez, Mireya DE LA Garza, Nidia Leon-Sicairos, Jose Antonio Garzon-Tiznado, Rossana Arroyo, Claudia Leon-Sicairos
In Entamoeba histolytica, iron modulates virulence and gene expression via unknown regulatory mechanisms. The existence of a posttranscriptional iron regulatory system parallel to the IRE/IRP (Iron-Responsive Element/Iron Regulatory Protein) system in the protozoan Trichomonas vaginalis has recently been reported. Due to their evolutionary closeness and the importance of iron for growth and virulence in these protozoa, we hypothesized the existence of an IRE/IRP-like mechanism in E. histolytica. To determine the presence of IRE-like elements in some mRNAs from this parasite, we performed in silico analyses of the 5'- and 3'-UTRs of mRNAs encoding virulence factors and cytoskeleton, ribosomal, and metabolism proteins...
January 18, 2017: Microbiology
https://www.readbyqxmd.com/read/28098912/microrna-9-limits-hepatic-fibrosis-by-suppressing-the-activation-and-proliferation-of-hepatic-stellate-cells-by-directly-targeting-mrp1-abcc1
#5
Jie Sun, Huanying Zhang, Liying Li, Lianfeng Yu, Lifang Fu
Liver fibrosis is a chronic liver disease characterized by the proliferation and activation of hepatic stellate cells (HSCs) and excessive deposition of extracellular matrix (ECM). Research suggests that microRNAs (miRNAs) are a new type of regulator of liver fibrosis. In the present study, we investigated the role of microRNA-9 (miR-9) in the process of liver fibrosis, as well as the underlying mechanism of action. Downregulated levels of miR-9 were found in fibrotic liver tissues and activated HSCs as detected by qRT-PCR; whereas, expression of multidrug resistance‑associated protein 1 (MRP1/ABCC1) was upregulated in the fibrotic liver tissues and activated HSCs...
January 17, 2017: Oncology Reports
https://www.readbyqxmd.com/read/28098870/mir-148a-increases-the-sensitivity-to-cisplatin-by-targeting-rab14-in-renal-cancer-cells
#6
Eun-Ae Kim, Tae Ghab Kim, Eon-Gi Sung, In-Hwan Song, Joo-Young Kim, Kyung-Oh Doh, Tae-Jin Lee
MicroRNA (miR) can exert various biological functions by targeting oncogenes or tumor suppressor genes in numerous human malignancies. Recent evidence has shown that miR-148a increases the drug sensitivity of various cancer cells. Herein, we show that ectopic expression of miR-148a induces apoptosis, reduces clonogenicity, and increases the sensitivity to TRAIL and cisplatin in renal cancer cells. The luciferase reporter assay showed that miR-148a negatively regulated ras-related protein 14 (Rab14) expression by binding to the miR-148a binding site in the 3' untranslated region (3'UTR) of Rab14...
January 17, 2017: International Journal of Oncology
https://www.readbyqxmd.com/read/28097495/the-expression-of-hypoxia-inducible-factor-1%C3%AE-gene-is-not-affected-by-low-oxygen-conditions-in-yellow-perch-perca-flavescens-juveniles
#7
Karolina Kwasek, Simona Rimoldi, Anna Giulia Cattaneo, Timothy Parker, Konrad Dabrowski, Genciana Terova
Hypoxia can affect various fish populations, including yellow perch Perca flavescens, which is an economically and ecologically important species in Lake Erie, a freshwater system that often experiences hypoxia in the hypolimnetic part of the lake. Fish, similarly to mammals, possess molecular oxygen sensor-hypoxia-inducible factor-1 (HIF-1), a transcription factor that can affect expression of many downstream genes related to animal growth and locomotion, protein synthesis, as well as ATP and amino acid metabolism...
January 18, 2017: Fish Physiology and Biochemistry
https://www.readbyqxmd.com/read/28097093/vascular-endothelial-growth-factor-suppresses-tnfsf15-production-in-endothelial-cells-by-stimulating-mir-31-and-mir-20a-expression-via-activation-of-akt-and-erk-signals
#8
Hui-Ting Deng, Hai-Lin Liu, Bei-Bei Zhai, Kun Zhang, Guo-Ce Xu, Xue-Mei Peng, Qiang-Zhe Zhang, Lu-Yuan Li
Tumor necrosis factor superfamily-15 (TNFSF15; VEGI; TL1A) is a negative modulator of angiogenesis for blood vessel homeostasis and is produced by endothelial cells in a mature vasculature. It is known to be downregulated by vascular endothelial growth factor (VEGF), a major regulator of neovascularization but the mechanism of this interaction is unclear. Here we report that VEGF is able to stimulate the production of two microRNAs, miR-20a and miR-31, which directly target the 3'-UTR of TNFSF15. Additionally, we show that two VEGF-stimulated cell growth signals, Erk and Akt, are responsible for promoting the expression of miR-20a and miR-31...
January 2017: FEBS Open Bio
https://www.readbyqxmd.com/read/28095464/molecular-control-of-innate-immune-response-to-pseudomonas-aeruginosa-infection-by-intestinal-let-7-in-caenorhabditis-elegans
#9
Lingtong Zhi, Yonglin Yu, Xueying Li, Daoyong Wang, Dayong Wang
The microRNA (miRNA) let-7 is an important miRNA identified in Caenorhabditis elegans and has been shown to be involved in the control of innate immunity. The underlying molecular mechanisms for let-7 regulation of innate immunity remain largely unclear. In this study, we investigated the molecular basis for intestinal let-7 in the regulation of innate immunity. Infection with Pseudomonas aeruginosa PA14 decreased let-7::GFP expression. Intestine- or neuron-specific activity of let-7 was required for its function in the regulation of innate immunity...
January 17, 2017: PLoS Pathogens
https://www.readbyqxmd.com/read/28094803/mir-124-inhibits-the-migration-and-invasion-of-human-hepatocellular-carcinoma-cells-by-suppressing-integrin-%C3%AE-v-expression
#10
Qian Qian Cai, Yi Wei Dong, Rong Wang, Bing Qi, Jun Xia Guo, Jing Pan, Yuan Yuan Liu, Chun Yi Zhang, Xing Zhong Wu
Tumor metastasis is the major cause of cancer-related death especially in human hepatocellular carcinoma (HCC). Although microRNAs have been implicated in tumor development, the roles of miR-124 in HCC metastasis are still not well understood. We conducted functional analysis in this study to investigate miR-124. We observed that miR-124 significantly retarded the wound healing and migration of HCC SMMC-7721 and BEL-7404 cells. Further analysis indicated miR-124 directly targeting the transcriptional factor Sp1 which is an important transcription factor for the integrin αV subunit gene transcription...
January 17, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28092654/promoter-polymorphism-of-toll-like-receptor-4-is-associated-with-a-decreased-risk-of-coronary-artery-disease-a-case-control-study-in-the-chinese-han-population
#11
Dandan Sun, Liping Sun, Qian Xu, Honghu Wang, Jun Yang, Yuan Yuan
BACKGROUND Coronary artery disease (CAD) is considered a chronic inflammatory disease of the blood vessels. Toll-like receptor 4 (TLR4) is a transmembrane receptor involved in inflammatory reactions. The aim of this study was to determine the association between polymorphisms in the promoter region and 3'-untranslated region (3'-UTR) of TLR4, and the associated CAD risk. MATERIAL AND METHODS This study enrolled 424 participants with CAD and 424 controls without CAD. The polymorphisms in the promoter region and 3'-UTR of TLR4 were identified from the HapMap database, including rs10116253, rs10983755, and rs11536889...
January 16, 2017: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
https://www.readbyqxmd.com/read/28089945/zearalenone-exposure-impairs-ovarian-primordial-follicle-formation-via-down-regulation-of-lhx8-expression-in-vitro
#12
Guo-Liang Zhang, Xiao-Feng Sun, Yan-Zhong Feng, Bo Li, Ya-Peng Li, Fan Yang, Charles Martin Nyachoti, Wei Shen, Shi-Duo Sun, Lan Li
Zearalenone (ZEA) is an estrogenic mycotoxin mainly produced as a secondary metabolite by numerous species of Fusarium. Previous work showed that ZEA had a negative impact on domestic animals with regard to reproduction. The adverse effects and the mechanisms of ZEA on mammalian ovarian folliculogenesis remain largely unknown, particularly its effect on primordial follicle formation. Thus, we investigated the biological effects of ZEA exposure on murine ovarian germ cell cyst breakdown and primordial follicle assembly...
January 12, 2017: Toxicology and Applied Pharmacology
https://www.readbyqxmd.com/read/28077561/micrornas-stimulate-translation-initiation-mediated-by-hcv-like-ireses
#13
Chloé Mengardi, Taran Limousin, Emiliano P Ricci, Ricardo Soto-Rifo, Didier Decimo, Théophile Ohlmann
MicroRNAs (miRNAs) are small non-coding RNAs that control gene expression by recognizing and hybridizing to a specific sequence generally located in the 3' untranslated region (UTR) of targeted mRNAs. miRNA-induced inhibition of translation occurs during the initiation step, most probably at the level of ribosome scanning. In this process, the RNA-induced silencing complex interacts both with PABP and the 43S pre-initiation complex to disrupt scanning of the 40S ribosome. However, in some specific cases, miRNAs can stimulate translation...
January 10, 2017: Nucleic Acids Research
https://www.readbyqxmd.com/read/28077174/mutant-hspb1-causes-loss-of-translational-repression-by-binding-to-pcbp1-an-rna-binding-protein-with-a-possible-role-in-neurodegenerative-disease
#14
Thomas Geuens, Vicky De Winter, Nicholas Rajan, Tilmann Achsel, Ligia Mateiu, Leonardo Almeida-Souza, Bob Asselbergh, Delphine Bouhy, Michaela Auer-Grumbach, Claudia Bagni, Vincent Timmerman
The small heat shock protein HSPB1 (Hsp27) is an ubiquitously expressed molecular chaperone able to regulate various cellular functions like actin dynamics, oxidative stress regulation and anti-apoptosis. So far disease causing mutations in HSPB1 have been associated with neurodegenerative diseases such as distal hereditary motor neuropathy, Charcot-Marie-Tooth disease and amyotrophic lateral sclerosis. Most mutations in HSPB1 target its highly conserved α-crystallin domain, while other mutations affect the C- or N-terminal regions or its promotor...
January 11, 2017: Acta Neuropathologica Communications
https://www.readbyqxmd.com/read/28077169/distinct-5-methylcytosine-profiles-in-poly-a-rna-from-mouse-embryonic-stem-cells-and-brain
#15
Thomas Amort, Dietmar Rieder, Alexandra Wille, Daria Khokhlova-Cubberley, Christian Riml, Lukas Trixl, Xi-Yu Jia, Ronald Micura, Alexandra Lusser
BACKGROUND: Recent work has identified and mapped a range of posttranscriptional modifications in mRNA, including methylation of the N6 and N1 positions in adenine, pseudouridylation, and methylation of carbon 5 in cytosine (m5C). However, knowledge about the prevalence and transcriptome-wide distribution of m5C is still extremely limited; thus, studies in different cell types, tissues, and organisms are needed to gain insight into possible functions of this modification and implications for other regulatory processes...
January 5, 2017: Genome Biology
https://www.readbyqxmd.com/read/28076852/mir-874-3p-is-down-regulated-in-hepatocellular-carcinoma-and-negatively-regulates-pin1-expression
#16
Ka-Wai Leong, Chi-Wai Cheng, Chun-Ming Wong, Irene Oi-Lin Ng, Yok-Lam Kwong, Eric Tse
PIN1 is a peptidyl-prolyl cis/trans isomerase (PPIase) that regulates multiple signaling pathways to control cell fate and is found to be over-expressed in cancers, including hepatocellular carcinoma (HCC). However, the regulation of PIN1 in HCC remains poorly defined. Micro-RNAs (miRNAs) have been reported to play a pivotal role in oncogenesis by targeting the 3'-untranslated region (UTR) of mRNAs encoded by oncogenes and tumour suppressor genes, thereby suppressing the levels of both oncoproteins and tumour suppressors...
January 5, 2017: Oncotarget
https://www.readbyqxmd.com/read/28075468/microrna-138-5p-controls-sensitivity-of-nasopharyngeal-carcinoma-to-radiation-by-targeting-eif4ebp1
#17
Wei Gao, Jacky Wei Kei Lam, John Zeng-Hong Li, Si-Qi Chen, Raymond King-Yin Tsang, Jimmy Yu-Wai Chan, Thian-Sze Wong
Radiation therapy is the standard treatment for primary nasopharyngeal carcinoma (NPC). MicroRNA regulates cancer responsiveness to radiation therapy by controlling the genes involved in radiation responses. Recent studies suggested that downregulation of microRNA-138-5p was clinically significant in NPC. Here, we evaluated the effect of miR-138-5p on radiosensitivity of NPC cells and explored the underlying mechanisms by identifying its target gene that impacted sensitivity to radiation. Our results revealed that overexpression of miR-138-5p reduced the ability to form colonies, inhibited proliferation, and enhanced radiation-induced DNA damage and autophagy in NPC cells upon radiation treatment...
February 2017: Oncology Reports
https://www.readbyqxmd.com/read/28074855/mir-218-targets-mecp2-and-inhibits-heroin-seeking-behavior
#18
Biao Yan, Zhaoyang Hu, Wenqing Yao, Qiumin Le, Bo Xu, Xing Liu, Lan Ma
MicroRNAs (miRNAs) are a class of evolutionarily conserved, 18-25 nucleotide non-coding sequences that post-transcriptionally regulate gene expression. Recent studies implicated their roles in the regulation of neuronal functions, such as learning, cognition and memory formation. Here we report that miR-218 inhibits heroin-induced behavioral plasticity. First, network propagation-based method was used to predict candidate miRNAs that played potential key roles in regulating drug addiction-related genes. Microarray screening was also carried out to identify miRNAs responding to chronic heroin administration in the nucleus accumbens (NAc)...
January 11, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28074308/influence-of-ts-and-abcb1%C3%A2-gene-polymorphisms-on-survival-outcomes-of-5%C3%A2-fu-based-chemotherapy-in-a%C3%A2-chinese-population-of-advanced-gastric-cancer-patients
#19
Jun Chen, Xueming Ying, Ling Zhang, Xiaojun Xiang, Jianping Xiong
To investigate the impacts of gene variations on survival outcomes of advanced gastric cancer (AGC) patients treated with 5‑fluorouracil (5-FU)-based chemotherapy, we analyzed the associations of 2 indels of the TS gene rs34743033 (double or triple tandem repeats of a 28 bp sequence in 5'-UTR, denoted as 2R or 3R allele) and rs16430 (a 6 bp variation at 1494 bp in 3'-UTR, denoted as ins6 or del6 allele) and 2 single nucleotide polymorphisms (SNPs) of ABCB1gene rs2032582 in exon 21 and rs1045642 in exon 26, with clinical outcomes after 5‑FU treatment...
January 10, 2017: Wiener Klinische Wochenschrift
https://www.readbyqxmd.com/read/28070856/mir-29c-prkci-regulates-axonal-growth-of-dorsal-root-ganglia-neurons-under-hyperglycemia
#20
Longfei Jia, Lei Wang, Michael Chopp, Chao Li, Yi Zhang, Alexandra Szalad, Zheng Gang Zhang
Diabetes initially induces distal axonal damage of peripheral nerves, but molecular mechanisms that mediate axonal injury are not fully understood. MircoRNAs (miRNAs) regulate axonal growth. We found that diabetic db/db mice exhibited substantial upregulation of miR-29c in dorsal root ganglia (DRG) neurons, sciatic nerve, and foot pad tissues. Bioinformatic analysis revealed PRKCI, a gene that encodes a member of the protein kinase C (PKC) iota, as a putative target for miR-29c. Western blot analysis showed that diabetic mice exhibited a considerable reduction of PRKCI protein levels in sciatic nerve tissues and DRG neurons...
January 9, 2017: Molecular Neurobiology
keyword
keyword
74313
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"