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https://www.readbyqxmd.com/read/28634044/mir-218-suppresses-gastric-cancer-cell-cycle-progression-through-the-cdk6-cyclin-d1-e2f1-axis-in-a-feedback-loop
#1
Min Deng, Chao Zeng, Xihong Lu, Xiusheng He, Ruixin Zhang, Qinwei Qiu, Guopei Zheng, Xiaoting Jia, Hao Liu, Zhimin He
Studies in several cancers have suggested that miR-218 has anti-tumor activities, but its function is yet to be elucidated. In this study, we investigated the regulation and function of miR-218 (miR-218-5p) in the cell cycle progression of gastric cancer (GC). We found that miR-218 could suppress proliferation of gastric cancer cells, induce cell cycle arrest at the G1 phase and inhibit tumor growth and metastasis in vivo. We also demonstrated that miR-218 specifically targeted the 3'-UTR regions of CDK6 and cyclin D1 and inhibited the expression of these molecules, which in turn repressesed the pRb/E2F1 signaling pathway...
June 17, 2017: Cancer Letters
https://www.readbyqxmd.com/read/28631392/-detection-of-negative-and-positive-rna-strand-of-poliovirus-sabin-1-and-echovirus-e19-by-a-stem-loop-reverse-transcription-pcr
#2
A Fikatas, T G Dimitriou, Z Kyriakopoulou, G Moschonas, G D Amoutzias, D Mossialos, C Gartzonika, S Levidiotou-Stefanou, P Markoulatos
In this report a strand specific RT-PCR was established for the detection of the replicative negative RNA strand of poliovirus sabin 1 (Sabin1) and Echovirus 19 (E19) strains. The key for the successful conduction of the assay was the use of a specific reverse transcription primer targeting the 5'-UTR of enteroviruses that consisted of a stem-loop structure at the 5'-end and an enteroviral-specific sequence at the 3'-end. The stem loop RT-PCR was found to be an accurate and sensitive method, detecting even 10(-2) CCID50 of poliovirus sabin 1 (Sabin1) and E19 strains 6(h) post infection (p...
June 20, 2017: Letters in Applied Microbiology
https://www.readbyqxmd.com/read/28630279/obesity-and-p16-ink4a-down-regulation-activate-breast-adipocytes-and-promote-their-pro-tumorigenicity
#3
Huda H Al-Khalaf, Mrad Amir, Falah Al-Mohanna, Asma Tulbah, Adher Al-Sayed, Abdelilah Aboussekhra
Obesity is increasingly recognized as a risk factor for breast cancer development. However, the molecular basis of obesity-related breast carcinogenesis remains elusive. In this study, we have shown that obesity reduces the level of the tumor suppressor p16(INK4A) protein in breast adipocytes, which showed active features and strong pro-carcinogenic potential both in vitro and in orthotopic tumor xenografts, as compared to mature adipocytes from lean women. Furthermore, obesity triggered epithelial-to-mesenchymal transition (EMT) in breast ductal epithelial cells...
June 19, 2017: Molecular and Cellular Biology
https://www.readbyqxmd.com/read/28628119/differential-expression-of-mir16-in-glioblastoma-and-glioblastoma-stem-cells-their-correlation-with-proliferation-differentiation-metastasis-and-prognosis
#4
R Tian, J Wang, H Yan, J Wu, Q Xu, X Zhan, Z Gui, M Ding, J He
The function of miR16 in multiforme glioblastoma multiforme (GBM) and its stem cells (GSCs) remains elusive. To this end, we investigated the patterns of miR16 expression in these cells and their correlation with malignant behaviors and clinical outcomes. The levels of miR16 and its targeted genes in tumor tissue of GBM and GBM SGH44, U87, U251 cells as well as their stem cell counterparts were measured by qRT-PCR or western blot or immunohistochemistry. Luciferase reporter assay was used to confirm the binding of miR16 to 3'-UTR of its target genes...
June 19, 2017: Oncogene
https://www.readbyqxmd.com/read/28627582/mir%C3%A2-27-inhibits-the-nf-%C3%AE%C2%BAb-signaling-pathway-by-targeting-leptin-in-osteoarthritic-chondrocytes
#5
Bin Zhou, Hongwei Li, Jide Shi
Osteoarthritis (OA) is a chronic degenerative joint disease. An increasing number of studies point to the role of microRNAs (miRNAs or miRs) in the pathogenesis of OA. An earlier study suggested that miR‑27b was associated with OA; however, the precise mechanisms regarding the involvement of the miR‑27 in the progression of OA remain unclear. In the present study, we first analyzed the effects of miR‑27 on OA. In vitro, the degree of miR‑27 expression was decreased in chondrocytes obtained from patients with OA...
June 13, 2017: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/28627026/two-nhx-type-transporters-from-helianthus-tuberosus-improve-the-tolerance-of-rice-to-salinity-and-nutrient-deficiency-stress
#6
Yang Zeng, Qing Li, Haiya Wang, Jianliang Zhang, Jia Du, Huimin Feng, Eduardo Blumwald, Ling Yu, Guohua Xu
The NHX-type cation/H(+) transporters in plants have been shown to mediate Na(+) (K(+) )/H(+) exchange for salinity tolerance and K(+) homeostasis. In this study, we identified and characterized two NHX homologues, HtNHX1 and HtNHX2 from an infertile and salinity tolerant species Helianthus tuberosus (cv. Nanyu No.1). HtNHX1 and HtNHX2 share identical 5'- and 3'-UTR and coding regions, except for a 342 bp segment encoding 114 amino acids (L272 to Q385 ) which is absent in HtNHX2. Both hydroponics and soil culture experiments showed that the expression of HtNHX1 or HtNHX2 improved the rice tolerance to salinity...
June 19, 2017: Plant Biotechnology Journal
https://www.readbyqxmd.com/read/28624790/co-targeting-of-igf1r-mtor-pathway-by-mir-497-and-mir-99a-impairs-hepatocellular-carcinoma-development
#7
Henghui Cheng, Jin Xue, Shouhua Yang, Yaobin Chen, Yu Wang, Yuanli Zhu, Xiaoyan Wang, Dong Kuang, Qiurong Ruan, Yaqi Duan, Guoping Wang
Persistent activation of IGF1R/mTOR signaling pathway plays crucial role in the development of hepatocellular carcinoma (HCC). Therefore, our goal was to elucidate microRNAs (miRNAs) targeting IGF1R/mTOR and the therapeutic potential of single or dual miRNA on HCC development. In this study, we found that miR-497 and miR-99a that target the 3'-UTR of both IGF1R and mTOR were down-regulated in HCC human tissues and cell lines. Functional assay revealed that ectopic expression of miR-497 or miR-99a in HCC cells resulted in a significant inhibition on tumor growth and invasiveness in vitro and tumor development in vivo via repressing the expression of IGF1R and mTOR...
May 24, 2017: Oncotarget
https://www.readbyqxmd.com/read/28624440/bag3-promotes-proliferation-of-ovarian-cancer-cells-via-post-transcriptional-regulation-of-skp2-expression
#8
Jing Yan, Chuan Liu, Jing-Yi Jiang, Hans Liu, Chao Li, Xin-Yu Li, Ye Yuan, Zhi-Hong Zong, Hua-Qin Wang
Bcl-2 associated athanogene 3 (BAG3) contains modular structure, through which BAG3 interacts with a wide range of proteins, thereby affording its capacity to regulate multifaceted biological processes. BAG3 is often highly expressed and functions as a pro-survival factor in many cancers. However, the oncogenic potential of BAG3 remains not fully understood. The cell cycle regulator, S-phase kinase associated protein 2 (Skp2) is increased in various cancers and plays an important role in tumorigenesis. The current study demonstrated that BAG3 promoted proliferation of ovarian cancer cells via upregulation of Skp2...
June 14, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28624222/targeting-dmpk-with-antisense-oligonucleotide-improves-muscle-strength-in-myotonic-dystrophy-type-1-mice
#9
Dominic Jauvin, Jessina Chrétien, Sanjay K Pandey, Laurie Martineau, Lucille Revillod, Guillaume Bassez, Aline Lachon, A Robert McLeod, Geneviève Gourdon, Thurman M Wheeler, Charles A Thornton, C Frank Bennett, Jack Puymirat
Myotonic dystrophy type 1 (DM1), a dominant hereditary muscular dystrophy, is caused by an abnormal expansion of a (CTG)n trinucleotide repeat in the 3' UTR of the human dystrophia myotonica protein kinase (DMPK) gene. As a consequence, mutant transcripts containing expanded CUG repeats are retained in nuclear foci and alter the function of splicing regulatory factors members of the MBNL and CELF families, resulting in alternative splicing misregulation of specific transcripts in affected DM1 tissues. In the present study, we treated DMSXL mice systemically with a 2'-4'-constrained, ethyl-modified (ISIS 486178) antisense oligonucleotide (ASO) targeted to the 3' UTR of the DMPK gene, which led to a 70% reduction in CUG(exp) RNA abundance and foci in different skeletal muscles and a 30% reduction in the heart...
June 16, 2017: Molecular Therapy. Nucleic Acids
https://www.readbyqxmd.com/read/28624205/atg7-overexpression-is-crucial-for-tumorigenic-growth-of-bladder-cancer-in%C3%A2-vitro-and-in%C3%A2-vivo-by-targeting-the-ets2-mirna196b-foxo1-p27-axis
#10
Junlan Zhu, Yang Li, Zhongxian Tian, Xiaohui Hua, Jiayan Gu, Jingxia Li, Claire Liu, Honglei Jin, Yulei Wang, Guosong Jiang, Haishan Huang, Chuanshu Huang
Human bladder cancer (BC) is the fourth most common cancer in the United States. Investigation of the strategies aiming to elucidate the tumor growth and metastatic pathways in BC is critical for the management of this disease. Here we found that ATG7 expression was remarkably elevated in human bladder urothelial carcinoma and N-butyl-N-(4-hydroxybutyl)nitrosamine (BBN)-induced mouse invasive BC. Knockdown of ATG7 resulted in a significant inhibitory effect on tumorigenic growth of human BC cells both in vitro and in vivo by promoting p27 expression and inducing cell cycle arrest at G2/M phase...
June 16, 2017: Molecular Therapy. Nucleic Acids
https://www.readbyqxmd.com/read/28624189/mir30c-is-involved-in-diabetic-cardiomyopathy-through-regulation-of-cardiac-autophagy-via-becn1
#11
Chen Chen, Shenglan Yang, Huaping Li, Zhongwei Yin, Jiahui Fan, Yanru Zhao, Wei Gong, Mengwen Yan, Dao Wen Wang
Multiple factors have been shown to promote the progression of diabetic cardiomyopathy. A link has previously been found between Mir30 and autophagy in cancer cells and in the heart, but the role of Mir30 in diabetic heart has not been studied. Using in vitro and in vivo approaches, we found that the depletion of Mir30c and induction of BECN1 enhanced autophagy in diabetic (db/db) hearts and in cardiomyocytes treated with the fatty acid palmitate. We verified that Mir30c repressed BECN1 expression by direct binding to the BECN1 3' UTRs...
June 16, 2017: Molecular Therapy. Nucleic Acids
https://www.readbyqxmd.com/read/28620193/microrna-29a-counteracts-synovitis-in-knee-osteoarthritis-pathogenesis-by-targeting-vegf
#12
Jih-Yang Ko, Mel S Lee, Wei-Shiung Lian, Wen-Tsan Weng, Yi-Chih Sun, Yu-Shan Chen, Feng-Sheng Wang
Synovitis contributes to the development of osteoarthritis (OA) of the knee. MicroRNAs regulate joint microenvironment homeostasis and deterioration. This study was undertaken to characterize the actions of microRNA-29a (miR-29a) to synovial remodeling in OA joints. Synovial specimens isolated from patients with end-stage OA knees showed abundant fibrotic matrix and vessel histopathology concomitant with weak miR-29a expression. In vitro, miR-29a knockdown caused synovial fibroblasts to exhibit high expressions of collagen III, TGF-β1, MMP9, MMP13, and ADAMTS5, whereas miR-29a overexpression diminished these joint-deleterious factors...
June 15, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28620087/daily-activity-of-the-housefly-musca-domestica-is-influenced-by-temperature-independent-of-3-utr-period-gene-splicing
#13
Olga Bazalova, David Dolezel
Circadian clocks orchestrate daily activity patterns and free running periods of locomotor activity under constant conditions. While the first often depends on temperature, the latter is temperature-compensated over a physiologically relevant range. Here, we explored the locomotor activity of the temperate housefly, Musca domestica Under low temperatures, activity was centered round a major and broad afternoon peak, while high temperatures resulted in activity throughout the photophase with a mild mid-day depression, which was especially pronounced in males exposed to long photoperiods...
June 15, 2017: G3: Genes—Genomes—Genetics
https://www.readbyqxmd.com/read/28619362/peroxiredoxin-1-from-cuttlefish-sepiella-maindroni-molecular-characterization-of-development-and-its-immune-response-against-vibrio-alginolyticus
#14
Weiwei Song, Changkao Mu, Ronghua Li, Chunlin Wang
The aim of this work was constructive to understand the function of peroxiredoxin (PRDX) family member Peroxiredoxin 1 in Sepiella maindroni (SmPrx1) through molecular mechanisms of reproduction, embryonic development and immune responses to Vibrio alginolyticus. The full-length cDNA of SmPrx1 was of 1062 bp, contains a 5' untranslated region (UTR) of 79bp, a 3' UTR of 359 bp, an open reading frame of 624 bp encoding 207 amino acids. The conserved peroxidase catalytic center "FYPLDFTFVCPTEI" and "GEVCPA" were observed in the sequence of SmPrx1; this indicated that it was a member of 2-Cys Prx...
June 12, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28618167/neuropilin-1-regulated-by-mir-320-contributes-to-the-growth-and-metastasis-of-cholangiocarcinoma-cells
#15
Huaqiang Zhu, Xian Jiang, Xu Zhou, Xuesong Dong, Kai Xie, Chuncheng Yang, Hongchi Jiang, Xueying Sun, Jun Lu
BACKGROUND: Neuropilin-1 (NRP-1) activates signaling pathways as multifunctional co-receptors in cancer cells. However, its role and how it is regulated by miRNAs in cholangiocarcinoma (CCA) have not yet been investigated. METHODS: The expression of NRP-1, miR-320 and key molecules involved in cell proliferation, migration and related signaling pathways were detected by immunohistochemistry, immunoblotting and qRT-PCR. Stable transfectants depleted of NRP-1 were generated...
June 15, 2017: Liver International: Official Journal of the International Association for the Study of the Liver
https://www.readbyqxmd.com/read/28617558/up-regulation-of-mir-124-inhibits-invasion-and-proliferation-of-prostate-cancer-cells-through-mediating-jak-stat3-signaling-pathway
#16
Z Wu, W Huang, B Chen, P-D Bai, X-G Wang, J-C Xing
OBJECTIVE: Signal transducer and activator of transcription 3 (STAT3) is an important protein in Janus kinase (JAK)-STAT signaling pathway, and can facilitate expression of Bcl-2 and Cyclin D1 gene, thus playing a role in tumor pathogenesis. Bioinformatics analysis revealed targeted binding sites between mircroRNA-124 (miR-124) and 3'-UTR of STAT3 mRNA. This study aims to investigate the role of miR-124 in regulating STAT3 expression and proliferation, cycle, apoptosis and invasion of prostate cancer cells...
May 2017: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/28615029/micrornas-control-mrna-fate-by-compartmentalization-based-on-3-utr-length-in-male-germ-cells
#17
Ying Zhang, Chong Tang, Tian Yu, Ruirui Zhang, Huili Zheng, Wei Yan
BACKGROUND: Post-transcriptional regulation of gene expression can be achieved through the control of mRNA stability, cytoplasmic compartmentalization, 3' UTR length and translational efficacy. Spermiogenesis, a process through which haploid male germ cells differentiate into spermatozoa, represents an ideal model for studying post-transcriptional regulation in vivo because it involves a large number of transcripts that are physically sequestered in ribonucleoprotein particles (RNPs) and thus subjected to delayed translation...
June 15, 2017: Genome Biology
https://www.readbyqxmd.com/read/28614186/mir-34c-5p-functions-as-pronociceptive-microrna-in-cancer-pain-by-targeting-cav2-3-containing-calcium-channels
#18
Jagadeesh Gandla, Santosh Kumar Lomada, Jianning Lu, Rohini Kuner, Kiran Kumar Bali
Pathophysiological mechanisms underlying pain associated with cancer are poorly understood. microRNAs are a class of non-coding RNAs with emerging functional importance in chronic pain. In a genome-wide screen for microRNAs regulated in dorsal root ganglia (DRG) neurons in a mouse model of bone metastatic pain, we identified miR-34c-5p as a functionally important pronociceptive microRNA. Despite these functional insights and therapeutic potential for miR-34c-5p, its molecular mechanism of action in peripheral sensory neurons remains unknown...
June 9, 2017: Pain
https://www.readbyqxmd.com/read/28614059/apigenin-sensitizes-bel-7402-adm-cells-to-doxorubicin-through-inhibiting-mir-101-nrf2-pathway
#19
Ai-Mei Gao, Xiao-Yu Zhang, Zun-Ping Ke
Chemo-resistance is one of the main obstacle in hepatocellular carcinoma therapy. Apigenin as a natural bioflavonoid has been exhibited anti-cancer properties in various malignant cancers. The aim of this study is to evaluate the potential chemo-sensitization effect of apigenin in doxorubicin-resistant hepatocellular carcinoma cell line BEL-7402/ADM and to investigate its possible mechanism. We found that apigenin significantly reversed doxorubicin sensitivity and induced caspase-dependent apoptosis in BEL-7402/ADM cells...
May 30, 2017: Oncotarget
https://www.readbyqxmd.com/read/28612854/fragile-x-mental-retardation-protein-recognizes-a-g-quadruplex-structure-within-the-survival-motor-neuron-domain-containing-1-mrna-5-utr
#20
Damian S McAninch, Ashley M Heinaman, Cara N Lang, Kathryn R Moss, Gary J Bassell, Mihaela Rita Mihailescu, Timothy L Evans
G quadruplex structures have been predicted by bioinformatics to form in the 5'- and 3'-untranslated regions (UTRs) of several thousand mature mRNAs and are believed to play a role in translation regulation. Elucidation of these roles has primarily been focused on the 3'-UTR, with limited focus on characterizing the G quadruplex structures and functions in the 5'-UTR. Investigation of the affinity and specificity of RNA binding proteins for 5'-UTR G quadruplexes and the resulting regulatory effects have also been limited...
June 14, 2017: Molecular BioSystems
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