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https://www.readbyqxmd.com/read/28323882/mir-181-interacts-with-signaling-adaptor-molecule-denn-madd-and-enhances-tnf-induced-cell-death
#1
Samira Ghorbani, Farideh Talebi, Sedigheh Ghasemi, Ali Jahanbazi Jahan Abad, Mohammed Vojgani, Farshid Noorbakhsh
MicroRNAs are small noncoding RNAs, which regulate the expression of protein coding transcripts through mRNA degradation or translational inhibition. Numerous reports have highlighted the role of miRNAs in regulating cell death pathways including the expression of genes involved in the induction of apoptosis. Tumor necrosis factor alpha (TNF-α) is a proinflammatory cytokine which can send pro-death signals through its receptor TNFR1. Diverse adaptor molecules including DENN/MADD adaptor protein have been shown to modulate TNF-α pro-death signaling via recruitment of MAP kinases to TNFR1 and activation of pro-survival NFκB signaling...
2017: PloS One
https://www.readbyqxmd.com/read/28320108/mir-103-regulates-triple-negative-breast-cancer-cells-migration-and-invasion-through-targeting-olfactomedin-4
#2
Bin Xiong, Xuefeng Lei, Lei Zhang, Jia Fu
Our previous study showed olfactomedin 4 (OLFM4) suppressed triple-negative breast cancer cells migration, invasion and metastasis-associated protein MMP 9 expression. OLFM4 was identified as a potential target of miR-103 according to microRNA target databases and published studies. The aim of this study is to validate the relationship between miR-103 and OLFM4, and explore the function and clinical significance of miR-103 in triple-negative breast cancer patients. In our results, miR-103 negatively regulated OLFM4 expression by directly targeting its 3'-UTR...
March 18, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28315363/sider2-retroposon-mediated-mrna-decay-in-leishmania-is-coupled-to-translation
#3
Hiva Azizi, Michaela Müller-McNicoll, Barbara Papadopoulou
We previously reported that Short Interspersed Degenerate Retroposons of the SIDER2 subfamily predominantly located within 3' untranslated regions (UTRs) of Leishmania transcripts promote rapid turnover that is initiated by endonucleolytic cleavage. Here, we investigated whether SIDER2-mediated decay is linked to translation. We show that preventing translation initiation by inserting a hairpin structure at the 5'-end of a SIDER2-containing mRNA blocks degradation. Similarly, global inhibition of translation elongation by cycloheximide or termination by puromycin causes stabilization of SIDER2-containing transcripts...
March 14, 2017: International Journal for Parasitology
https://www.readbyqxmd.com/read/28314168/mir-137-downregulates-c-kit-expression-in-acute-myeloid-leukemia
#4
Yanping Hu, Xiaolong Dong, Guoming Chu, Guangrui Lai, Bijun Zhang, Leitong Wang, Yanyan Zhao
The oncogene c-kit plays a vital role in the pathogenesis of acute myeloid leukemia (AML). However, the mechanism of microRNAs targeting c-kit in AML has not been determined in detail. Moreover, the role miR-137 in tumor cell proliferation remains controversial. The aim of this work was to verify whether miR-137 targets c-kit and to research the biological effects of restoring miR-137 expression in leukemia cells. We found that miR-137 binds specifically to the 3'-UTR of c-kit and suppresses the expression and activities of c-kit...
February 16, 2017: Leukemia Research
https://www.readbyqxmd.com/read/28302726/the-rna-binding-protein-tristetraprolin-ttp-is-a-critical-negative-regulator-of-the-nlrp3-inflammasome
#5
Moritz Haneklaus, John D O'Neil, Andrew R Clark, Seth L Masters, Luke A J O'Neill
The NLRP3 inflammasome is a central regulator of inflammation in many common diseases, including atherosclerosis and Type 2 diabetes, driving the production of pro-inflammatory mediators such as IL-1β and IL-18. Due to its function as an inflammatory gatekeeper, expression and activation of NLRP3 need to be tightly regulated. In this study, we highlight novel post-transcriptional mechanisms that can modulate NLRP3 expression. We have identified the RNA-binding protein Tristetraprolin (TTP) as a negative regulator of NLRP3 in human macrophages...
March 16, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28298907/mir-21a-5p-contributes-to-porcine-hemagglutinating-encephalomyelitis-virus-proliferation-via-targeting-cask-interactive-protein1-in-vivo-and-vitro
#6
Xiaoling Lv, Kui Zhao, Yungang Lan, Zi Li, Ning Ding, Jingjing Su, Huijun Lu, Deguang Song, Feng Gao, Wenqi He
Porcine hemagglutinating encephalomyelitis virus (PHEV) is a highly neurovirulent coronavirus that can cause nervous symptoms in piglets with muscle tremors, hind limb paralysis, and nystagmus. Whether some factors affect virus replication and proliferation had not been fully understood in the course of nerve damage caused by PHEV infection. In recent years, some reports suggested that miRNA might play a key regulatory role in viral infection. In this study, we found the miR-21a-5p is notably up-regulated in the brains of mice and N2a cells infected with PHEV, and it down-regulated the expression of CASK-interactive protein1 (Caskin1) by directly targeting the 3'-UTR of Caskin1 using a Dual-Luciferase reporter assay...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28298474/tumor-protein-d52-expression-is-post-transcriptionally-regulated-by-t-cell-intercellular-antigen-tia-1-and-tia-related-protein-via-mrna-stability
#7
Hiromi Motohashi, Yoshiki Mukudai, Chihiro Ito, Kosuke Kato, Toshikazu Shimane, Seiji Kondo, Tatsuo Shirota
Although tumor protein D52 (TPD) family proteins were first identified nearly 20 years ago, their molecular regulatory mechanisms remain unclear. Therefore, we investigated the post-transcriptional regulation of TPD52 family genes. An RNA immunoprecipitation assay showed the potential binding ability of TPD52 family mRNAs to several RNA-binding proteins, and an RNA degradation assay revealed that TPD52 is subject to more prominent post-transcriptional regulation than TPD53 and 54. We subsequently focused on the 3'-untranslated region (3'-UTR) of TPD52 as a cis -acting element in post-transcriptional gene regulation...
March 15, 2017: Biochemical Journal
https://www.readbyqxmd.com/read/28298306/il-1%C3%AE-induces-nf-%C3%AE%C2%BAb-and-upregulates-microrna-372-to-inhibit-spinal-cord-injury-recovery
#8
Wei Zhou, Tongzhou Yuan, Youshui Gao, Peipei Yin, Wei Liu, Chenhao Pan, Yingjie Liu, Xiaowei Yu
Excessive inflammation including IL-1β-initiated signaling is among the earlies reactions that can cause neuronal damage following spinal cord injury (SCI). It has been suggested that microRNAs may participate in stem cell repair to facilitate functional recovery after SCI. Here we showed that in cultured human neural stem cells (hNSC) IL-1β reduced the expressions of both KIF3B (kinesin family member 3B) and NOSIP (nitric oxide synthase interacting protein), two key modulators for restricting inflammation and promoting neuronal regeneration...
March 15, 2017: Journal of Neurophysiology
https://www.readbyqxmd.com/read/28294412/association-of-758-g-a-polymorphism-of-3-untranslated-region-of-prohibitin-with-risk-of-gastric-cancer
#9
Fenfen Xiang, Zhenhua Ni, Yueping Zhan, Jian Xu, Rong Wu, Xiangdong Kang
BACKGROUND: A polymorphic variant allele (T-allele) in the 3'-UTR of prohibitin (C-to-T at nucleotide 729) was reported to be associated with an increased risk of breast cancer. However, the association between the 3'-UTR polymorphism of prohibitin and the susceptibility to gastric cancer remains unknown. Thus, we investigated the distribution of prohibitin genotypes in Chinese patients with gastric cancer and subsequently analyzed the association between the 3'-UTR polymorphism of prohibitin and the risk of gastric cancer in that population...
March 13, 2017: Journal of Clinical Laboratory Analysis
https://www.readbyqxmd.com/read/28294375/identification-of-the-interaction-between-bta-mir-370-and-olr1-gene-in-bovine-adipocyte
#10
H F Li, S H Wang, Y Guo, H B Zhao, X Y Li, X Wang
It has been shown that the oxidized low density lipoprotein receptor 1 (OLR1) gene plays an important role in the degradation of oxidized low density lipoprotein. Previous studies found a SNP in the 3'-untranslated region (3'-UTR) of the OLR1 gene associated with milk production traits in different dairy cattle populations and with loin eye area and marbling depth in beef cattle. MicroRNAs can regulate gene expression by binding the 3'-UTR of target genes to degrade or to repress the translation of target genes...
March 10, 2017: Animal Genetics
https://www.readbyqxmd.com/read/28294061/the-association-of-mme-microrna-binding-site-polymorphism-with-risk-of-late-onset-alzheimer-s-disease-in-northern-han-chinese
#11
Chun-Xia Liu, Lin Tan, Fu-Rong Sun, Wei Zhang, Dan Miao, Meng-Shan Tan, Yu Wan, Chen-Chen Tan, Jin-Tai Yu, Lan Tan
Background Although amyloid β (Aβ) degradation has been normally implicated in the pathogenesis of late onset Alzheimer's disease (LOAD) through cellular biological studies, genetic studies linking Aβ degradation and LOAD are still not deeply investigated. Neprilysin (NEP), as one of the most crucial Aβ-degrading enzymes in AD, is the metalloendopeptidases which particularly participates in the monomeric Aβ species degradation. MicroRNAs (miRNAs) exert posttranscriptional dysregulation and their target sequence on the 3' untranslated regions (3'UTR) may be regulated by single nucleotide polymorphisms (SNPs)...
March 13, 2017: Current Neurovascular Research
https://www.readbyqxmd.com/read/28291810/microrna-532-and-microrna-3064-inhibit-cell-proliferation-and-invasion-by-acting-as-direct-regulators-of-human-telomerase-reverse-transcriptase-in-ovarian-cancer
#12
Lin Bai, Hui Wang, Ai-Hua Wang, Luo-Ying Zhang, Jie Bai
Human telomerase reverse transcriptase (hTERT) plays a crucial role in ovarian cancer (OC) progression. However, the mechanisms underlying hTERT upregulation in OC, and the specific microRNAs (miRNAs) involved in the regulation of hTERT in OC cells, remains unclear. We performed a bioinformatics search to identify potential miRNAs that bind to the 3'-untranslated region (3'-UTR) region of the hTERT mRNA. We examined the expression levels of miR-532/miR-3064 in OC tissues and normal ovarian tissues, and analyzed the correlation between miRNA expression and OC patient outcomes...
2017: PloS One
https://www.readbyqxmd.com/read/28290615/mir-214-regulates-oral-cancer-kb-cell-apoptosis-through-targeting-rassf5
#13
T K Li, K Yin, Z Chen, Y Bao, S X Zhang
Ras association domain family member 5 (RASSF5), a member of the Ras association domain family, induces cell apoptosis by phosphorylating FOXO3a, which triggers target gene BIM (pro-apoptotic factor) activation. MiR-214 is overexpressed in oral cancer tissue, indicating its possible involvement in oral cancer pathogenesis. Bioinformatics analysis has revealed a complimentary sequence between miR-214 and the 3'-UTR of RASSF5 mRNA. However, whether miR-124 regulates RASSF5 in oral cancer remains poorly understood...
March 8, 2017: Genetics and Molecular Research: GMR
https://www.readbyqxmd.com/read/28289143/transcription-factor-wilms-tumor-1-regulates-developmental-rnas-through-3-utr-interaction
#14
Ruthrothaselvi Bharathavikru, Tatiana Dudnakova, Stuart Aitken, Joan Slight, Mara Artibani, Peter Hohenstein, David Tollervey, Nick Hastie
Wilms' tumor 1 (WT1) is essential for the development and homeostasis of multiple mesodermal tissues. Despite evidence for post-transcriptional roles, no endogenous WT1 target RNAs exist. Using RNA immunoprecipitation and UV cross-linking, we show that WT1 binds preferentially to 3' untranslated regions (UTRs) of developmental targets. These target mRNAs are down-regulated upon WT1 depletion in cell culture and developing kidney mesenchyme. Wt1 deletion leads to rapid turnover of specific mRNAs. WT1 regulates reporter gene expression through interaction with 3' UTR-binding sites...
March 13, 2017: Genes & Development
https://www.readbyqxmd.com/read/28288910/characterization-and-functional-analysis-of-a-novel-c-type-lectin-from-the-swimming-crab-portunus-trituberculatus
#15
Junkai Lu, Zuoben Yu, Changkao Mu, Ronghua Li, Weiwei Song, Chunlin Wang
C-type lectins (CTLs) are a family of calcium-dependent carbohydrate-binding proteins. In the present study, a novel C-type lectin (designated as PtCTL1) was identified and characterized from Portunus trituberculatus. The full-length cDNA of PtCTL1 was of 702 bp, containing a 5' untranslated region (UTR) of 91 bp, a 3' UTR of 110 bp with a poly (A) tail, and an open reading frame (ORF) of 501 bp encoding a polypeptide of 166 amino acids with a putative signaling peptide of 21 amino acids. A C-type lectin carbohydrate-recognition domain (CRD) containing four conserved cysteines was identified in the amino acid sequence of PtCTL1...
March 10, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28288641/sox2-regulates-multiple-malignant-processes-of-breast-cancer-development-through-the-sox2-mir-181a-5p-mir-30e-5p-tusc3-axis
#16
Kuancan Liu, Fuan Xie, Anding Gao, Rui Zhang, Long Zhang, Zhangwu Xiao, Qiong Hu, Weifeng Huang, Qiaojia Huang, Baoshun Lin, Jian Zhu, Haikun Wang, Jianwen Que, Xiaopeng Lan
BACKGROUND: High levels of SOX2 protein are correlated with increased dissemination of breast cancer. However, the underlying molecular mechanisms are not fully understood. METHODS: In this study we investigate the role of SOX2 in breast cancer metastasis using multiple in vitro and in vivo assays including cell culture, shRNA-mediated knockdown, wound healing, colony formation, transwell chamber, xenograft and tail vein injection. Moreover, western blot, immunostaining, microarray and real-time PCR were used to determine the change of protein and miRNA levels...
March 14, 2017: Molecular Cancer
https://www.readbyqxmd.com/read/28287144/mir-153-regulates-amelogenesis-by-targeting-endocytotic-and-endosomal-lysosomal-pathways-novel-insight-into-the-origins-of-enamel-pathologies
#17
Kaifeng Yin, Wenting Lin, Jing Guo, Toshihiro Sugiyama, Malcolm L Snead, Joseph G Hacia, Michael L Paine
Amelogenesis imperfecta (AI) is group of inherited disorders resulting in enamel pathologies. The involvement of epigenetic regulation in the pathogenesis of AI is yet to be clarified due to a lack of knowledge about amelogenesis. Our previous genome-wide microRNA and mRNA transcriptome analyses suggest a key role for miR-153 in endosome/lysosome-related pathways during amelogenesis. Here we show that miR-153 is significantly downregulated in maturation ameloblasts compared with secretory ameloblasts. Within ameloblast-like cells, upregulation of miR-153 results in the downregulation of its predicted targets including Cltc, Lamp1, Clcn4 and Slc4a4, and a number of miRNAs implicated in endocytotic pathways...
March 13, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28287067/translational-control-of-mrnas-by-3-untranslated-region-binding-proteins
#18
Akio Yamashita, Osamu Takeuchi
Eukaryotic gene expression is precisely regulated at all points between transcription and translation. In this review, we focus on translational control mediated by the 3'-untranslated regions (UTRs) of mRNAs. mRNA 3'-UTRs contain cis-acting elements that function in the regulation of protein translation or mRNA decay. RNA binding proteins that bind to these cis-acting elements regulate mRNA translation via various mechanisms targeting the mRNA cap structure, the eukaryotic initiation factor 4E (eIF4E)-eIF4G complex, ribosomes, and the poly (A) tail...
March 13, 2017: BMB Reports
https://www.readbyqxmd.com/read/28286314/a-norepinephrine-responsive-mirna-directly-promotes-cghsp90aa1-expression-in-oyster-haemocytes-during-desiccation
#19
Hao Chen, Lusheng Xin, Xiaorui Song, Lin Wang, Weilin Wang, Zhaoqun Liu, Huan Zhang, Lingling Wang, Zhi Zhou, Limei Qiu, Linsheng Song
Oyster Crassostrea gigas is one model mollusc inhabiting in the intertidal zone and is frequently stressed by desiccation. The adaptation mechanism of oyster to environmental stress involves multiple levels, and miRNA is one of the most important regulators in post-transcriptional level. In the present study, an oyster norepinephrine-responsive miRNA cgi-miR-365 was proved to contribute to the host adaptation against desiccation by directly promoting the expression of CgHSP90AA1. Briefly, a significant increase of cgi-miR-365 was observed from the first day after aerial exposure and the up-regulation was vigorously repressed when oysters were injected with adrenoceptors antagonists...
March 9, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28284149/microrna-93-alleviates-neuropathic-pain-through-targeting-signal-transducer-and-activator-of-transcription-3
#20
Xue-Tao Yan, Li-Juan Ji, Zhiyu Wang, Xingjun Wu, Quan Wang, Shujie Sun, Jing-Min Lu, Yang Zhang
Emerging evidence suggests that microRNAs (miRNAs) play a critical role in the pathogenesis of neuropathic pain. However, the exact role of miRNAs in regulating neuropathic pain remains largely unknown. In this study, we aimed to investigate the potential role of miR-93 in a rat model of neuropathic pain induced by chronic constriction sciatic nerve injury (CCI). We found a significant decrease of miR-93 in the spinal cord of CCI rats compared with sham rats. Overexpression of miR-93 significantly alleviated neuropathic pain development and reduced inflammatory cytokine expression, including interleukin (IL)-1β, tumor necrosis factor (TNF)-α, and IL-6 in CCI rats...
March 8, 2017: International Immunopharmacology
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