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https://www.readbyqxmd.com/read/28076691/removal-of-copper-from-aqueous-solutions-by-rhizofiltration-using-genetically-modified-hairy-roots-expressing-a-bacterial-cu-binding-protein
#1
Patricia Pérez-Palacios, Elizabeth Agostini, Sabrina G Ibáñez, Melina A Talano, Ignacio D Rodríguez-Llorente, Miguel A Caviedes, Eloísa Pajuelo
The aim of this work was developing a biotechnological tool to hyperaccumulate high copper (Cu) concentrations from wastewaters. Transgenic tobacco hairy roots were obtained by expressing, either the wild type version of the gene copC from Pseudomonas fluorescens in the cytoplasm of plant cells (CuHR), or a modified version targeted to the vacuole (CuHR-V). Control hairy roots transformed with the empty vector (HR) were also generated. The roots were incubated in the presence of solutions containing Cu (from 1 to 50 mM)...
January 11, 2017: Environmental Technology
https://www.readbyqxmd.com/read/28070655/synthetic-introns-help-identify-sequences-in-the-5-utr-intron-of-the-glycine-max-polyubiquitin-gmubi-promoter-that-give-increased-promoter-activity
#2
Trudi N L Grant, Carola M De La Torre, Ning Zhang, John J Finer
Specific sequences within the leader intron of a soybean polyubiquitin gene stimulated gene expression when placed either within a synthetic intron or upstream of a core promoter. The intron in the 5' untranslated region of the soybean polyubiquitin promoter, Gmubi, seems to contribute to the high activity of this promoter. To identify the stimulatory sequences within the intron, ten different sequential intronic sequences of 40 nt were isolated, cloned as tetrameric repeats and placed upstream of a minimal cauliflower mosaic virus 35S (35S) core promoter, which was used to control expression of the green fluorescent protein...
January 9, 2017: Planta
https://www.readbyqxmd.com/read/28052433/diverse-functions-of-multidrug-and-toxin-extrusion-mate-transporters-in-citric-acid-efflux-and-metal-homeostasis-in-medicago-truncatula
#3
Junjie Wang, Qiuqiang Hou, Penghui Li, Lina Yang, Xuecheng Sun, Vagner A Benedito, Jiangqi Wen, Beibei Chen, Kirankumar S Mysore, Jian Zhao
The MATE transporter family comprises 70 members in the Medicago truncatula genome, and they play seemingly important, yet mostly uncharacterized, physiological functions. Here, we employed bioinformatics and molecular genetics to identify and characterize MATE transporters involved in citric acid export, Al(3+) tolerance and Fe translocation. MtMATE69 is a citric acid transporter induced by Fe-deficiency. Overexpression of MtMATE69 in hairy roots altered Fe homeostasis and hormone levels under Fe-deficient or Fe-oversupplied conditions...
January 4, 2017: Plant Journal: for Cell and Molecular Biology
https://www.readbyqxmd.com/read/28025661/erratum-to-increasing-the-synthesis-of-bioactive-abietane-diterpenes-in-salvia-sclarea-hairy-roots-by-elicited-transcriptional-reprogramming
#4
M C Vaccaro, A Mariaevelina, N Malafronte, N De Tommasi, A Leone
No abstract text is available yet for this article.
December 27, 2016: Plant Cell Reports
https://www.readbyqxmd.com/read/28018397/function-of-ap2-erf-transcription-factors-involved-in-the-regulation-of-specialized-metabolism-in-ophiorrhiza-pumila-revealed-by-transcriptomics-and-metabolomics
#5
Nirin Udomsom, Amit Rai, Hideyuki Suzuki, Jun Okuyama, Ryosuke Imai, Tetsuya Mori, Ryo Nakabayashi, Kazuki Saito, Mami Yamazaki
The hairy roots (HR) of Ophiorrhiza pumila produce camptothecin (CPT), a monoterpenoid indole alkaloid used as a precursor in the synthesis of chemotherapeutic drugs. O. pumila HR culture is considered as a promising alternative source of CPT, however, the knowledge about the biosynthetic pathway and regulatory mechanism is still limited. In this study, five genes that encode AP2/ERF transcription factors, namely OpERF1-OpERF5, were isolated from HR of O. pumila. Phylogenetic analysis of AP2/ERF protein sequences suggested the close evolutionary relationship of OpERF1 with stress-responsive ERF factors in Arabidopsis and of OpERF2 with ERF factors reported to regulate alkaloid production, such as ORCA3 in Catharanthus roseus, NIC2 locus ERF in tobacco, and JRE4 in tomato...
2016: Frontiers in Plant Science
https://www.readbyqxmd.com/read/27975997/glycyrrhizin-production-in-hairy-root-cultures-of-glycyrrhiza-uralensis-induced-triterpenoid-biosynthetic-gene
#6
M Kojoma, H Seki, K Ohyama, S Y Kim, T Muranaka
No abstract text is available yet for this article.
December 2016: Planta Medica
https://www.readbyqxmd.com/read/27975983/establishment-of-hairy-root-cultures-of-chili-peppers-capsicum-annuum-using-different-explants
#7
B Pongkitwitoon, A Pitirattanaworranat, S Prathanturarug
No abstract text is available yet for this article.
December 2016: Planta Medica
https://www.readbyqxmd.com/read/27973421/enhanced-stilbene-production-and-excretion-in-vitis-vinifera-cv-pinot-noir-hairy-root-cultures
#8
Leo-Paul Tisserant, Aziz Aziz, Nathalie Jullian, Philippe Jeandet, Christophe Clément, Eric Courot, Michèle Boitel-Conti
Stilbenes are defense molecules produced by grapevine in response to stresses including various elicitors and signal molecules. Together with their prominent role in planta, stilbenes have been the center of much attention in recent decades due to their pharmaceutical properties. With the aim of setting up a cost-effective and high purity production of resveratrol derivatives, hairy root lines were established from Vitis vinifera cv Pinot Noir 40024 to study the organ-specific production of various stilbenes...
December 10, 2016: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/27943249/metabolic-engineering-of-fatty-alcohol-production-in-transgenic-hairy-roots-of-crambe-abyssinica
#9
Magdalena Miklaszewska, Antoni Banaś, Aleksandra Królicka
Biotechnological production of fatty alcohols, important raw materials in the chemical industry, has been receiving considerable attention in recent years. Fatty alcohols are formed by the reduction of fatty acyl-CoAs or fatty acyl-ACPs catalyzed by a fatty acyl reductase (FAR). In this study, we introduced genes encoding FARs from Arabidopsis thaliana (AtFAR5) and Simmondsia chinensis (ScFAR) into Crambe abyssinica hairy roots via Agrobacterium rhizogenes-mediated transformation. The efficiency of the transformation ranged between 30-45%...
December 12, 2016: Biotechnology and Bioengineering
https://www.readbyqxmd.com/read/27941644/host-derived-artificial-microrna-as-an-alternative-method-to-improve-soybean-resistance-to-soybean-cyst-nematode
#10
Bin Tian, Jiarui Li, Thomas R Oakley, Timothy C Todd, Harold N Trick
The soybean cyst nematode (SCN), Heterodera glycines, is one of the most important pests limiting soybean production worldwide. Novel approaches to managing this pest have focused on gene silencing of target nematode sequences using RNA interference (RNAi). With the discovery of endogenous microRNAs as a mode of gene regulation in plants, artificial microRNA (amiRNA) methods have become an alternative method for gene silencing, with the advantage that they can lead to more specific silencing of target genes than traditional RNAi vectors...
December 8, 2016: Genes
https://www.readbyqxmd.com/read/27933900/-13-c-nmr-and-lc-ms-profiling-of-stilbenes-from-elicited-grapevine-hairy-root-cultures
#11
Leo-Paul Tisserant, Jane Hubert, Michelle Lequart, Nicolas Borie, Nicolas Maurin, Serge Pilard, Philippe Jeandet, Aziz Aziz, Jean-Hugues Renault, Jean-Marc Nuzillard, Christophe Clément, Michèle Boitel-Conti, Eric Courot
Resveratrol and related oligostilbenes are defense molecules produced by grapevine in response to stresses including various elicitors or signal molecules. Together with their prominent role in planta, these compounds have been the center of much attention in recent decades due to their pharmacological properties. The cost-effective production of resveratrol derivatives such as viniferins or more structurally complex stilbene oligomers remains a challenging task. In this study, the chemical diversity of stilbenes produced by Vitis vinifera Pinot Noir hairy roots was investigated after elicitation for 4 days with a mixture of methyl jasmonate (100 μM) and cyclodextrins (50 mM)...
November 23, 2016: Journal of Natural Products
https://www.readbyqxmd.com/read/27898492/growth-hormone-regulates-the-sensitization-of-developing-peripheral-nociceptors-during-cutaneous-inflammation
#12
Xiaohua Liu, Kathryn J Green, Zachary K Ford, Luis F Queme, Peilin Lu, Jessica L Ross, Frank B Lee, Aaron T Shank, Renita C Hudgins, Michael P Jankowski
Cutaneous inflammation alters the function of primary afferents and gene expression in the affected dorsal root ganglia (DRG). However, specific mechanisms of injury-induced peripheral afferent sensitization and behavioral hypersensitivity during development are not fully understood. Recent studies in children suggest a potential role for growth hormone (GH) in pain modulation. Growth hormone modulates homeostasis and tissue repair after injury, but how GH affects nociception in neonates is not known. To determine whether GH played a role in modulating sensory neuron function and hyperresponsiveness during skin inflammation in young mice, we examined behavioral hypersensitivity and the response properties of cutaneous afferents using an ex vivo hairy skin-saphenous nerve-DRG-spinal cord preparation...
February 2017: Pain
https://www.readbyqxmd.com/read/27898105/chemical-induction-of-hairpin-rnai-molecules-to-silence-vital-genes-in-plant-roots
#13
Siming Liu, John I Yoder
Understanding the functions encoded by plant genes can be facilitated by reducing transcript levels by hairpin RNA (hpRNA) mediated silencing. A bottleneck to this technology occurs when a gene encodes a phenotype that is necessary for cell viability and silencing the gene inhibits transformation. Here we compared the use of two chemically inducible plant promoter systems to drive hpRNA mediated gene silencing in transgenic, hairy roots. We cloned the gene encoding the Yellow Fluorescence Protein (YFP) into the dexamethasone inducible vector pOpOff2 and into the estradiol induced vector pER8...
November 29, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27871141/root-isoflavonoids-and-hairy-root-transformation-influence-key-bacterial-taxa-in-the-soybean-rhizosphere
#14
Laura J White, Xijin Ge, Volker S Brözel, Senthil Subramanian
Rhizodeposits play a key role in shaping rhizosphere microbial communities. In soybean, isoflavonoids are a key rhizodeposit component that aid in plant defense and enable symbiotic associations with rhizobia. However, it is uncertain if and how they influence rhizosphere microbial communities. Isoflavonoid biosynthesis was silenced via RNA interference of isoflavone synthase in soybean hairy root composite plants. Rhizosphere soil fractions tightly associated with roots were isolated, and PCR amplicons from 16S rRNA gene variable regions V1-V3 and V3-V5 from these fractions were sequenced using 454...
November 21, 2016: Environmental Microbiology
https://www.readbyqxmd.com/read/27853836/increasing-the-synthesis-of-bioactive-abietane-diterpenes-in-salvia-sclarea-hairy-roots-by-elicited-transcriptional-reprogramming
#15
M C Vaccaro, A Mariaevelina, N Malafronte, N De Tommasi, A Leone
Transcriptional activation of genes belonging to the plastidial MEP-derived isoprenoid pathway by elicitation with methyl jasmonate and coronatine enhanced the content of bioactive abietane diterpenes in Salvia sclarea hairy roots. We have shown that aethiopinone, an abietane diterpene synthesized in Salvia sclarea roots is cytotoxic and induces apoptosis in human melanoma cells. To develop a production platform for this compound and other abietane diterpenes, hairy root technology was combined with the elicitation of methyl jasmonate (MeJA) or the phytotoxin coronatine (Cor)...
November 16, 2016: Plant Cell Reports
https://www.readbyqxmd.com/read/27815671/integrating-qtl-mapping-and-transcriptomics-identifies-candidate-genes-underlying-qtls-associated-with-soybean-tolerance-to-low-phosphorus-stress
#16
Dan Zhang, Hengyou Zhang, Shanshan Chu, Hongyan Li, Yingjun Chi, Daniella Triebwasser-Freese, Haiyan Lv, Deyue Yu
Soybean is a high phosphorus (P) demand species that is sensitive to low-P stress. Although many quantitative trait loci (QTL) for P efficiency have been identified in soybean, but few of these have been cloned and agriculturally applied mainly due to various limitations on identifying suitable P efficiency candidate genes. Here, we combined QTL mapping, transcriptome profiling, and plant transformation to identify candidate genes underlying QTLs associated with low-P tolerance and response mechanisms to low-P stress in soybean...
November 4, 2016: Plant Molecular Biology
https://www.readbyqxmd.com/read/27813337/still-stable-after-11-years-a-catharanthus-roseus-hairy-root-line-maintains-inducible-expression-of-anthranilate-synthase
#17
Jiayi Sun, Li Ma, Ka-Yiu San, Christie A M Peebles
Hairy root cultures generated using Agrobacterium rhizogenes are an extensively investigated system for the overproduction of various secondary metabolite based pharmaceuticals and chemicals. This study demonstrated a transgenic Catharanthus roseus hairy root line carrying a feedback-insensitive anthranilate synthase (AS) maintained chemical and genetic stability for 11 years. The AS gene was originally inserted in the hairy root genome under the control of a glucocorticoid inducible promoter. After 11 years continuous maintenance of this hairy root line, genomic PCR of the ASA gene showed the presence of ASA gene in the genome...
November 4, 2016: Biotechnology Progress
https://www.readbyqxmd.com/read/27809756/recent-patents-in-agricultural-biotechnology-focus-on-health
#18
Abdullah Makhzoum, Srividhya Venkataraman, Jocelyne Tremouillaux-Guiller, Kathleen L Hefferon
BACKGROUND: Agricultural biotechnology, including the generation of genetically modified food crops, has been the subject of much controversy over the last few years. Initially serving the basic needs of farmers, Ag Biotech has more recently gained much appeal for its opportunities with respect to both the nutritional and pharmaceutical sciences. METHODS: The following review describes a number of recently approved patents that could have direct implications for the field of medicine...
November 1, 2016: Recent Patents on Food, Nutrition & Agriculture
https://www.readbyqxmd.com/read/27806110/a-leader-intron-of-a-soybean-elongation-factor-1a-eef1a-gene-interacts-with-proximal-promoter-elements-to-regulate-gene-expression-in-synthetic-promoters
#19
Ning Zhang, Leah K McHale, John J Finer
Introns, especially the first intron in the 5' untranslated region (5'UTR), can significantly impact gene expression via intron-mediated enhancement (IME). In this study, we demonstrate the leader intron of a soybean elongation factor 1A (eEF1A) gene (GmScreamM8) was essential for the high activity of the native promoter. Furthermore, the interaction of the GmScreamM8 leader intron with regulatory element sequences from several soybean eEF1A promoters was studied using synthetic promoters, which consisted of element tetramers upstream of a core promoter used to regulate a green fluorescent protein (gfp) reporter gene...
2016: PloS One
https://www.readbyqxmd.com/read/27801944/a-differentially-regulated-ap2-erf-transcription-factor-gene-cluster-acts-downstream-of-a-map-kinase-cascade-to-modulate-terpenoid-indole-alkaloid-biosynthesis-in-catharanthus-roseus
#20
Priyanka Paul, Sanjay K Singh, Barunava Patra, Xueyi Sui, Sitakanta Pattanaik, Ling Yuan
Catharanthus roseus produces bioactive terpenoid indole alkaloids (TIAs), including the chemotherapeutics, vincristine and vinblastine. Transcriptional regulation of TIA biosynthesis is not fully understood. The jasmonic acid (JA)-responsive AP2/ERF transcription factor (TF), ORCA3, and its regulator, CrMYC2, play key roles in TIA biosynthesis. ORCA3 forms a physical cluster with two uncharacterized AP2/ERFs, ORCA4 and 5. Here, we report that (1) the ORCA gene cluster is differentially regulated; (2) ORCA4, while overlapping functionally with ORCA3, modulates an additional set of TIA genes...
November 1, 2016: New Phytologist
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