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https://www.readbyqxmd.com/read/29140457/genistein-specific-g6dt-gene-for-the-inducible-production-of-wighteone-in-lotus-japonicus
#1
Jinyue Liu, Wenbo Jiang, Yaying Xia, Xuemin Wang, Guoan Shen, Yongzhen Pang
Prenylated isoflavonoids were found in several legume plants, and they possess various biological activities that play important roles in both plant defense and human health. However, it is still unknown whether prenylated isoflavonoids are present in the model legume plant L. japonicus. In the present study, we found that the prenylated isoflavonoid wighteone was produced in L. japonicus when the leaf was supplemented with genistein. Furthermore, a novel prenyltransferase gene LjG6DT was identified, which shared high similarity and was closely related with several known prenyltransferase genes involved in isoflavonoid biosynthesis...
November 10, 2017: Plant & Cell Physiology
https://www.readbyqxmd.com/read/29139179/involvement-of-lemrp-an-atp-binding-cassette-transporter-in-shikonin-transport-and-biosynthesis-in-lithospermum-erythrorhizon
#2
Yu Zhu, Shu-Juan Chu, Yue-Lin Luo, Jiang-Yan Fu, Cheng-Yi Tang, Gui-Hua Lu, Yan-Jun Pang, Xiao-Ming Wang, Rong-Wu Yang, Jin-Liang Qi, Yong-Hua Yang
·Shikonin and its derivatives are important medicinal secondary metabolites accumulating in the roots of Lithospermum erythrorhizon. Although some membrane proteins have been identified as transporters of secondary metabolites, the mechanisms underlying shikonin transport and accumulation in L. erythrorhizon cells still remain largely unknown. ·In this study, we isolated a cDNA encoding LeMRP, an ATP-binding cassette transporter from L. erythrorhizon and further investigated its functions in the transport and biosynthesis of shikonin with the yeast transformation and transgenic hairy root method, respectively...
November 15, 2017: Plant Biology
https://www.readbyqxmd.com/read/29132307/involvement-of-lemdr-an-atp-binding-cassette-protein-gene-in-shikonin-transport-and-biosynthesis-in-lithospermum-erythrorhizon
#3
Yu Zhu, Gui-Hua Lu, Zhuo-Wu Bian, Feng-Yao Wu, Yan-Jun Pang, Xiao-Ming Wang, Rong-Wu Yang, Cheng-Yi Tang, Jin-Liang Qi, Yong-Hua Yang
BACKGROUND: Shikonin is a naphthoquinone secondary metabolite with important medicinal value and is found in Lithospermum erythrorhizon. Considering the limited knowledge on the membrane transport mechanism of shikonin, this study investigated such molecular mechanism. RESULTS: We successfully isolated an ATP-binding cassette protein gene, LeMDR, from L. erythrorhizon. LeMDR is predominantly expressed in L. erythrorhizon roots, where shikonin accumulated. Functional analysis of LeMDR by using the yeast cell expression system revealed that LeMDR is possibly involved in the shikonin efflux transport...
November 13, 2017: BMC Plant Biology
https://www.readbyqxmd.com/read/29105731/expression-of-tabersonine-16-hydroxylase-and-16-hydroxytabersonine-o-methyltransferase-in-catharanthus-roseus-hairy-roots
#4
Jiayi Sun, Le Zhao, Zengyi Shao, Jacqueline Shanks, Christie A M Peebles
The monoterpene indole alkaloids vindoline and catharanthine, which are exclusively synthesized in the medicinal plant Catharanthus roseus, are the two important precursors for the production of pharmaceutically important anti-cancer medicines vinblastine and vincristine. Hairy root culture is an ideal platform for alkaloids production due to its industrial scalability, genetic and chemical stability, and availability of genetic engineering tools. However, C. roseus hairy roots do not produce vindoline due to the lack of expression of the seven-step pathway from tabersonine to vindoline (Murata and De Luca, 2005)...
November 4, 2017: Biotechnology and Bioengineering
https://www.readbyqxmd.com/read/29098590/identification-and-characterization-of-key-circadian-clock-genes-of-tobacco-hairy-roots-putative-regulatory-role-in-xenobiotic-metabolism
#5
Lucas G Sosa Alderete, Mario E Guido, Elizabeth Agostini, Paloma Mas
The circadian clock is an endogenous system that allows organisms to daily adapt and optimize their physiology and metabolism. We studied the key circadian clock gene (CCG) orthologs in Nicotiana tabacum seedlings and in hairy root cultures (HRC). Putative genes involved in the metabolism of xenobiotic compounds (MXC) were selected and their expression profiles were also analyzed. Seedlings and HRC displayed similar diurnal variations in the expression profiles for the CCG examined under control conditions (CC)...
November 3, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/29079721/diverse-peptide-hormones-affecting-root-growth-identified-in-the-medicago-truncatula-secreted-peptidome
#6
Neha Patel, Nadiatul A Mohd-Radzman, Leo Corcilius, Ben Crossett, Angela Connolly, Stuart J Cordwell, Ariel Ivanovici, Katia Taylor, James Wlilliams, Steve Binos, Michael Mariani, Richard J Payne, Michael A Djordjevic
Multigene families encoding diverse secreted peptide hormones play important roles in plant development. A need exists to efficiently elucidate the structures and post-translational modifications of these difficult-to-isolate peptide hormones in planta so that their biological functions can be determined. A mass spectrometry and bioinformatics approach was developed to comprehensively analyse the secreted peptidome of Medicago hairy root cultures and xylem sap. We identified 759 spectra corresponding to the secreted products of twelve peptide hormones including four CEP (C-TERMINALLY ENCODED PEPTIDE), two CLE (CLV3/ENDOSPERM SURROUNDING REGION RELATED) and six XAP (XYLEM SAP ASSOCIATED PEPTIDE) peptides...
October 27, 2017: Molecular & Cellular Proteomics: MCP
https://www.readbyqxmd.com/read/29065529/immobilization-of-lead-migrating-from-contaminated-soil-in-rhizosphere-soil-of-barley-hordeum-vulgare-l-and-hairy-vetch-vicia-villosa-using-hydroxyapatite
#7
Masahiko Katoh, Elsya Risky, Takeshi Sato
This study conducted plant growth tests using a rhizobox system to quantitatively determine the distance of immobilization lead migrating from contaminated soil into uncontaminated rhizosphere soil, and to assess the lead phases accumulated in rhizosphere soil by sequential extraction. Without the hydroxyapatite, exchangeable lead fractions increased as the rhizosphere soil got closer to the contaminated soil. Exchangeable lead fractions were higher even in the rhizosphere soil that shares a boundary with the root surface than in the soil before being planted...
October 23, 2017: International Journal of Environmental Research and Public Health
https://www.readbyqxmd.com/read/29063187/composite-poplars-a-novel-tool-for-ectomycorrhizal-research
#8
Dimitri Neb, Arpita Das, Annette Hintelmann, Uwe Nehls
Composite poplars were used for ectomycorrhiza formation. Structurally normal mycorrhizas of transgenic roots revealed better fungal sugar support. Targeting fluorescent proteins to peroxisomes allowed easy in planta visualization of successful transformation. A bottle neck in ectomycorrhizal research is the time demand for generation of transgenic plants. An alternative strategy for such root-centered research might be the formation of the so-called composite plants, where transgenic roots are formed by non-transgenic shoots...
December 2017: Plant Cell Reports
https://www.readbyqxmd.com/read/29045000/characterization-of-the-soybean-gmalmt-family-genes-and-the-function-of-gmalmt5-in-response-to-phosphate-starvation
#9
Wenting Peng, Weiwei Wu, Junchu Peng, Jiaojiao Li, Yan Lin, Yanan Wang, Jiang Tian, Lili Sun, Cuiyue Liang, Hong Liao
A potential mechanism to enhance utilization of sparingly soluble forms of phosphorus (P) is the root secretion of malate, which is mainly mediated by the ALMT gene family in plants. In this study, a total of 34 GmALMT genes were identified in the soybean genome. Expression patterns diverged considerably among GmALMTs in response to phosphate (Pi) starvation in leaves, roots and flowers, with expression altered by P availability in 26 of the 34 GmALMTs. One root-specific GmALMT whose expression was significantly enhanced by Pi-starvation, GmALMT5, was studied in more detail to determine its possible role in soybean P nutrition...
October 17, 2017: Journal of Integrative Plant Biology
https://www.readbyqxmd.com/read/28992334/gmphr25-a-gmphr-member-up-regulated-by-phosphate-starvation-controls-phosphate-homeostasis-in-soybean
#10
Ying-Bin Xue, Bi-Xian Xiao, Sheng-Nan Zhu, Xiao-Hui Mo, Cui-Yue Liang, Jiang Tian, Hong Liao
As an essential nutrient element, phosphorus (P) plays an important role in plant growth and development. Low P availability is a limiting factor for crop production, especially for legume crops (e.g. soybean), which require additional P to sustain nitrogen fixation through symbiotic associations with rhizobia. Although PHOSPHATE STARVATION RESPONSE 1 (PHR1) or PHR1-like is considered as a central regulator of phosphate (Pi) homeostasis in several plant species, it remains undefined in soybean. In this study, 35 GmPHR members were cloned from the soybean genome and expression patterns in soybean were assayed under nitrogen (N) and P deficiency conditions...
August 23, 2017: Journal of Experimental Botany
https://www.readbyqxmd.com/read/28979275/soybean-lec2-regulates-subsets-of-genes-involved-in-controlling-the-biosynthesis-and-catabolism-of-seed-storage-substances-and-seed-development
#11
Sehrish Manan, Muhammad Z Ahmad, Gaoyang Zhang, Beibei Chen, Basir U Haq, Jihong Yang, Jian Zhao
Soybean is an important oilseed crop and major dietary protein resource, yet the molecular processes and regulatory mechanisms involved in biosynthesis of seed storage substances are not fully understood. The B3 domain transcription factor (TF) LEC2 essentially regulates embryo development and seed maturation in other plants, but is not functionally characterized in soybean. Here, we characterize the function of a soybean LEC2 homolog, GmLEC2a, in regulating carbohydrate catabolism, triacylglycerol (TAG) biosynthesis, and seed development...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28966626/natural-agrobacterium-transformants-recent-results-and-some-theoretical-considerations
#12
REVIEW
Ke Chen, Léon Otten
Agrobacterium rhizogenes causes hairy root growth on a large number of plant species. It does so by transferring specific DNA fragments (T-DNA) from its root-inducing plasmid (pRi) into plant cells. Expression of T-DNA genes leads to abnormal root growth and production of specific metabolites (opines) which are taken up by the bacterium and used for its growth. Recent work has shown that several Nicotiana, Linaria, and Ipomoea species contain T-DNA genes from A. rhizogenes in their genomes. Plants carrying such T-DNAs (called cellular T-DNA or cT-DNA) can be considered as natural transformants...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28957691/differential-rubisco-content-and-photosynthetic-efficiency-of-rol-gene-integrated-vinca-minor-transgenic-plant-correlating-factors-associated-with-morpho-anatomical-changes-gene-expression-and-alkaloid-productivity
#13
Priyanka Verma, Shamshad Ahmad Khan, Nusrat Masood, N Manika, Abhishek Sharma, Neha Verma, Suaib Luqman, Ajay K Mathur
Transgenic plants obtained from a hairy root line (PVG) of Vinca minor were characterized in relation to terpenoid indole alkaloids (TIAs) pathway gene expression and vincamine production. The hairy roots formed callus with green nodular protuberances when transferred onto agar-gelled MS medium containing 3.0mg/l zeatin. These meristematic zones developed into shoot buds on medium with 1.0mg/l 2, 4-dichlorophenoxyacetic acid and 40mg/l ascorbic acid. These shoot buds subsequently formed rooted plants when shifted onto a hormone-free MS medium with 6% sucrose...
September 20, 2017: Journal of Plant Physiology
https://www.readbyqxmd.com/read/28956383/-factors-influencing-induction-and-in-vitro-culture-of-hairy-roots-in-phytolacca-americana-l
#14
Heping Shi, Yuanfeng Zhu, Po Keung Eric Tsang, Cheuk Fai Stephen Chow, Zhen'ao Yu, Shengqin Huang
To use hairy roots for producing medicinal ingredients of Phytolacca americana L. we studied the factors influencing the induction and in vitro culture. Hairy roots could be incited from the veins of cut surface (morphological lower) of P. americana L. leaf explants around 18 days after infection with the strain of Agrobacterium rhizogenes ATCC15834. The highest rooting rate, 70%, was obtained when leaf explants were pre-cultured for 1 day, infected for 20 min, and co-cultured for 4 days. The transformation was confirmed by PCR amplification of rolC of Ri plasmid and silica gel thin-layer chromatography of opines from P...
February 25, 2017: Sheng Wu Gong Cheng Xue Bao, Chinese Journal of Biotechnology
https://www.readbyqxmd.com/read/28953930/molecular-cloning-and-characterization-of-five-smgras-genes-associated-with-tanshinone-biosynthesis-in-salvia-miltiorrhiza-hairy-roots
#15
Zhenqing Bai, Pengguo Xia, Ruilin Wang, Jie Jiao, Mei Ru, Jingling Liu, Zongsuo Liang
The gibberellin-responsive element binding factor (GRAS) family of proteins plays an important role in the transcriptional regulation of plant development and hormone signaling. To date, there are no reports on GRAS family proteins expressed in Salvia miltiorrhiza. In this study, 28 ESTs that contained the GRAS domain were identified from a S. miltiorrhiza cDNA library. Of these, full-length sequences of five genes were cloned and sequence analysis indicated that all five proteins contain only one GRAS domain and therefore, belong to the GRAS family...
2017: PloS One
https://www.readbyqxmd.com/read/28950698/enhanced-production-of-two-bioactive-isoflavone-aglycones-in-astragalus-membranaceus-hairy-root-cultures-by-combining-deglycosylation-and-elicitation-of-immobilized-edible-aspergillus-niger
#16
Jiao Jiao, Qing-Yan Gai, Li-Li Niu, Xi-Qing Wang, Na Guo, Yu-Ping Zang, Yu-Jie Fu
A cocultivation system of Astragalus membranaceus hairy root cultures (AMHRCs) and immobilized food-grade fungi was established for the enhanced production of calycosin (CA) and formononetin (FO). The highest accumulations of CA (730.88 ± 63.72 μg/g DW) and FO (1119.42 ± 95.85 μg/g DW) were achieved in 34 day-old AMHRCs cocultured with immobilized A. niger (IAN) for 54 h, which were 7.72- and 18.78-fold higher than CA and FO in nontreated control, respectively. IAN deglycosylation could promote the formation of CA and FO by conversion of their glycoside precursors...
October 5, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28937992/efficient-generation-of-mutations-mediated-by-crispr-cas9-in-the-hairy-root-transformation-system-of-brassica-carinata
#17
Thomas W Kirchner, Markus Niehaus, Thomas Debener, Manfred K Schenk, Marco Herde
A protocol for the induction of site-directed deletions and insertions in the genome of Brassica carinata with CRISPR is described. The construct containing the Cas9 nuclease and the guide RNA (gRNA) was delivered by the hairy root transformation technique, and a successful transformation was monitored by GFP fluorescence. PAGE analysis of an amplified region, presumably containing the deletions and insertions, demonstrated up to seven different indels in one transgenic root and in all analyzed roots a wildtype allele of the modified gene was not detectable...
2017: PloS One
https://www.readbyqxmd.com/read/28933110/-effect-of-lanthanum-on-accumulation-of-active-constituent-and-key-enzymes-expression-of-salvia-miltiorrhiza-hairy-root
#18
Li-Hua Bian, Lin Zou, Bing-Qian Zhou, Wei Liu, Jie Zhou, Xiao Wang
The effect of Lanthanum on the accumulation of active constituent and key enzymes expression of Salvia miltiorrhiza hairy root were studied and furthermore signaling molecules mediating the synthesis of secondary metabolism was also defined in order to provide references for the reveal of synthesis mechanism of active constituent of S. miltiorrhiza hairy root inducing by Lanthanum. The content of active constituents were detected by HPLC. RNA was extracted with RNA prep Pure RNA purification kit (Tiangen). The results shows that LaCl3 processing promoted the accumulation of tanshinones and phenolic acids in S...
December 2016: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
https://www.readbyqxmd.com/read/28922763/chicory-r2r3-myb-transcription-factors-cimyb5-and-cimyb3-regulate-fructan-1-exohydrolase-expression-in-response-to-abiotic-stress-and-hormonal-cues
#19
Hongbin Wei, Hongbo Zhao, Tao Su, Anja Bausewein, Steffen Greiner, Karsten Harms, Thomas Rausch
In the biennial Cichorium intybus, inulin-type fructans accumulate in the taproot during the first year. Upon cold or drought exposure, fructans are degraded by fructan exohydrolases, affecting inulin yield and degree of polymerization. While stress-induced expression of 1-FEH genes has been thoroughly explored, the transcriptional network mediating these responses has remained unknown. In this study, several R2R3-MYB transcriptional regulators were analysed for their possible involvement in 1-FEH regulation via transient transactivation of 1-FEH target promoters and for in vivo co-expression with target genes under different stress and hormone treatments...
July 10, 2017: Journal of Experimental Botany
https://www.readbyqxmd.com/read/28921050/characterization-of-ccstop1-a-c2h2-type-transcription-factor-regulates-al-tolerance-gene-in-pigeonpea
#20
Abhijit Arun Daspute, Yuriko Kobayashi, Sanjib Kumar Panda, Bashasab Fakrudin, Yasufumi Kobayashi, Mutsutomo Tokizawa, Satoshi Iuchi, Arbind Kumar Choudhary, Yoshiharu Y Yamamoto, Hiroyuki Koyama
Al-responsive citrate-transporting CcMATE1 function and its regulation by CcSTOP1 were analyzed using NtSTOP1 -KD tobacco- and pigeonpea hairy roots, respectively, CcSTOP1 binding sequence of CcMATE1 showed similarity with AtALMT1 promoter. The molecular mechanisms of Aluminum (Al) tolerance in pigeonpea (Cajanus cajan) were characterized to provide information for molecular breeding. Al-inducible citrate excretion was associated with the expression of MULTIDRUGS AND TOXIC COMPOUNDS EXCLUSION (CcMATE1), which encodes a citrate transporter...
September 18, 2017: Planta
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