keyword
MENU ▼
Read by QxMD icon Read
search

hairy root

keyword
https://www.readbyqxmd.com/read/28733871/a-mdr-transporter-contributes-to-the-different-extracellular-production-of-sesquiterpene-pyridine-alkaloids-between-adventitious-root-and-hairy-root-liquid-cultures-of-tripterygium-wilfordii-hook-f
#1
Guo-Peng Miao, Juan Han, Ji-Feng Zhang, Chuan-Shu Zhu, Xing Zhang
TwMDR1 transports sesquiterpene pyridine alkaloids, wilforine and wilforgine, into the hairy roots of T. wilfordii Hook.f. resulting in low secretion ratio of alkaloids. Hairy roots (HRs) exhibit high growth rate and biochemical and genetic stability. However, varying secondary metabolites in HR liquid cultures mainly remain in root tissues, and this condition may affect cell growth and cause inconvenience in downstream extraction. Studies pay less attention to adventitious root (AR) liquid cultures though release ratio of some metabolites in AR liquid cultures is significantly higher than that of HR...
July 21, 2017: Plant Molecular Biology
https://www.readbyqxmd.com/read/28722263/ftsad2-and-ftjaz1-regulate-activity-of-the-ftmyb11-transcription-repressor-of-the-phenylpropanoid-pathway-in-fagopyrum-tataricum
#2
Meiliang Zhou, Zhanmin Sun, Mengqi Ding, Maria D Logacheva, Ivan Kreft, Dan Wang, Mingli Yan, Jirong Shao, Yixiong Tang, Yanmin Wu, Xuemei Zhu
Little is known about the molecular mechanism of the R2R3-MYB transcriptional repressors involved in plant phenylpropanoid metabolism. Here, we describe one R2R3-type MYB repressor, FtMYB11 from Fagopyrum tataricum. It contains the SID-like motif GGDFNFDL and it is regulated by both the importin protein 'Sensitive to ABA and Drought 2' (SAD2) and the jasmonates signalling cascade repressor JAZ protein. Yeast two hybrid and bimolecular fluorescence complementation assays demonstrated that FtMYB11 interacts with SAD2 and FtJAZ1...
July 19, 2017: New Phytologist
https://www.readbyqxmd.com/read/28722156/chemometric-evaluation-of-hypericin-and-related-phytochemicals-in-17-in-vitro-cultured-hypericum-species-hairy-root-cultures-and-hairy-root-derived-transgenic-plants
#3
Katarína Nigutová, Souvik Kusari, Selahaddin Sezgin, Linda Petijová, Jana Henzelyová, Miroslava Bálintová, Michael Spiteller, Eva Čellárová
OBJECTIVES: The objective of this study was to ascertain the presence and correlations among eight important secondary metabolites viz. hypericin, pseudohypericin, emodin, hyperforin, rutin, hyperoside, quercetin and quercitrin in different organs of 17 in vitro cultured Hypericum species, along with H. tomentosum and H. tetrapterum hairy root cultures, and hairy root-derived transgenic plants of H. tomentosum. METHODS: Samples were extracted and analysed by LC-MS...
July 19, 2017: Journal of Pharmacy and Pharmacology
https://www.readbyqxmd.com/read/28714761/gamma-irradiation-of-medicinally-important-plants-and-the-enhancement-of-secondary-metabolite-production
#4
P Vivek Vardhan, Lata I Shukla
PURPOSE: The profitable production of some important plant-based secondary metabolites (ginsenosides, saponins, camptothecin, shikonins etc.) in vitro by gamma irradiation is a current area of interest. We reviewed different types of secondary metabolites, their mode of synthesis and effect of γ-radiation on their yield for different plants, organs and in vitro cultures (callus, suspension, hairy root). Special effort has been made to review the biochemical mechanisms underlying the increase in secondary metabolites...
July 17, 2017: International Journal of Radiation Biology
https://www.readbyqxmd.com/read/28709614/generation-of-recombinant-protein-shells-of-johnson-grass-chlorotic-stripe-mosaic-virus-in-tobacco-plants-and-their-use-as-drug-carrier
#5
E Alemzadeh, K Izadpanah, F Ahmadi
The development and use of virus-like particles (VLPs) is a growing field with a powerful potential in generation of nanoparticles. In the present study we have attempted to generate and use empty shells of Johnson grass chlorotic stripe mosaic virus (JgCSMV, a member of the genus Aureusvirus, family Tombusviridae) as VLP nanoparticles for drug loading. In order to successfully produce recombinant JgCSMV-derived VLPs, we followed an approach based on cloning of the JgCSMV CP gene into pBI121 vector and introduction of the latter into Agrobacterium rhizogenes and transformation of tobacco cells for coat protein expression...
July 12, 2017: Journal of Virological Methods
https://www.readbyqxmd.com/read/28707113/genetically-engineered-hairy-root-cultures-of-hyoscyamus-senecionis-and-h-muticus-ploidy-as-a-promising-parameter-in-the-metabolic-engineering-of-tropane-alkaloids
#6
Esmaeil Dehghan, Darwin W Reed, Patrick S Covello, Zeinab Hasanpour, Javier Palazon, Kirsi-Marja Oksman-Caldentey, Farajollah Shahriari Ahmadi
Tetraploidy improves overexpression of h6h and scopolamine production of H. muticus, while in H. senecionis, pmt overexpression and elicitation can be used as effective methods for increasing tropane alkaloids. The effects of metabolic engineering in a polyploid context were studied by overexpression of h6h in the tetraploid hairy root cultures of H. muticus. Flow cytometry analysis indicated genetic stability in the majority of the clones, while only a few clones showed genetic instability. Among all the diploid and tetraploid clones, the highest level of h6h transgene expression and scopolamine accumulation was interestingly observed in the tetraploid clones of H...
July 13, 2017: Plant Cell Reports
https://www.readbyqxmd.com/read/28702035/the-soybean-basic-helix-loop-helix-transcription-factor-org3-like-enhances-cadmium-tolerance-via-increased-iron-and-reduced-cadmium-uptake-and-transport-from-roots-to-shoots
#7
Zhaolong Xu, Xiaoqing Liu, Xiaolan He, Ling Xu, Yihong Huang, Hongbo Shao, Dayong Zhang, Boping Tang, Hongxiang Ma
Cadmium (Cd) is one of the most dangerous heavy metal pollutants in the environment and is toxic to animal and plant cells. On the other hand, iron (Fe) is an essential element for plant growth and development. The chlorosis of plant leaves under cadmium stress is similar to the typical symptom of iron deficiency. Recently, several Arabidopsis basic/helix-loop-helix (bHLH) transcription factors have been identified that are involved in the interactions between Cd and Fe. In the present study, over-expression the ORG3-like gene GmORG3, a bHLH transcription factor OBP3-responsive gene (ORG), enhanced Cd tolerance and stabilized Fe homeostasis...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28698552/smmyb36-a-novel-r2r3-myb-transcription-factor-enhances-tanshinone-accumulation-and-decreases-phenolic-acid-content-in-salvia-miltiorrhiza-hairy-roots
#8
Kai Ding, Tianlin Pei, Zhengqing Bai, Yanyan Jia, Pengda Ma, Zongsuo Liang
Phenolic acids and tanshinones are two major bioactive components in Salvia miltiorrhiza Bunge. A novel endogenous R2R3-MYB transcription factor, SmMYB36, was identified in this research. This transcript factor can simultaneously influence the content of two types of components in SmMYB36 overexpression hairy roots. SmMYB36 was mainly localized in the nucleus of onion epidermis and it has transactivation activity. The overexpression of SmMYB36 promoted tanshinone accumulation but inhibited phenolic acid and flavonoid biosynthesis in Salvia miltiorrhiza hairy roots...
July 11, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28694825/rapid-separation-of-indole-glucosinolates-in-roots-of-chinese-cabbage-brassica-rapa-subsp-pekinensis-by-high-performance-liquid-chromatography-with-diode-array-detection
#9
Alfredo Aires, Rosa Carvalho
Glucosinolates are a class of sulphur-containing plant compounds with diverse biological properties. They have been found exclusively in the Brassicaceae family plants and studied exhaustively in biosynthetic and application perspectives. The aim of this current study is to present a simple and updated method to quantify indole glucosinolate content in hairy root cultures of Chinese cabbage by HPLC-DAD-UV/Vis. Method validation controls were performed and recovery experiments were assayed. The data was statically treated and compared with published works...
2017: International Journal of Analytical Chemistry
https://www.readbyqxmd.com/read/28690624/transcriptome-wide-identification-of-novel-uv-b-and-light-modulated-flavonol-pathway-genes-controlled-by-vvimybf1
#10
Stefan Czemmel, Janine Höll, Rodrigo Loyola, Patricio Arce-Johnson, José Antonio Alcalde, José Tomás Matus, Jochen Bogs
Flavonols constitute a group of flavonoids with important photoprotective roles in plants. In addition, flavonol content and composition greatly influences fruit quality. We previously demonstrated that the grapevine R2R3-MYB transcription factor (TF) VviMYBF1 promotes flavonol accumulation by inducing the expression of flavonol synthase (VviFLS1/VviFLS4), a key step of the initial flavonol pathway. Despite this, gene networks underlying flavonol modification in grapevine including both structural and regulatory genes remain poorly understood...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28687763/a-tyrosine-aminotransferase-involved-in-rosmarinic-acid-biosynthesis-in-prunella-vulgaris-l
#11
Mei Ru, Kunru Wang, Zhenqing Bai, Liang Peng, Shaoxuan He, Yong Wang, Zongsuo Liang
Rosmarinic acid (RA) and its derivants are medicinal compounds that comprise the active components of several therapeutics. We isolated and characterised a tyrosine aminotransferase of Prunella vulgaris (PvTAT). Deduced PvTAT was markedly homologous to other known/putative plant TATs. Cytoplasmic localisation of PvTAT was observed in tobacco protoplasts. Recombinantly expressed and purified PvTAT had substrates preference for L-tyrosine and phenylpyruvate, with apparent K m of 0.40 and 0.48 mM, and favoured the conversion of tyrosine to 4-hydroxyphenylpyruvate...
July 7, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28670794/brassica-rapa-hairy-root-extracts-promote-skin-depigmentation-by-modulating-melanin-production-and-distribution
#12
Luigi Michele Sena, Claudia Zappelli, Fabio Apone, Ani Barbulova, Annalisa Tito, Antonella Leone, Teresa Oliviero, Rosalia Ferracane, Vincenzo Fogliano, Gabriella Colucci
BACKGROUND: Skin whitening products, used for ages by Asian people for cultural and esthetic purposes, are very popular nowadays in Western countries as well, where the need to inhibit skin spots after sun exposure has become not only a cosmetic but also a health-related issue. Thus, the development of effective and safe depigmenting agents derived from natural products gets continuous attention by cosmetic brands and consumers. OBJECTIVES: The aim of this study was to determine the effects of two preparations, obtained from the hairy root cultures of the species Brassica rapa, on melanogenesis and the expression of the extracellular matrix proteins involved in a correct pigment distribution...
July 3, 2017: Journal of Cosmetic Dermatology
https://www.readbyqxmd.com/read/28659950/mapping-and-characterization-of-the-fefe-gene-that-controls-iron-uptake-in-melon-cucumis-melo-l
#13
Raghuprakash Kastoori Ramamurthy, Brian M Waters
Iron (Fe) deficiency in plants limits crop growth and productivity. Molecular mechanisms that plants use to sense and respond to Fe deficiency by coordinated expression of Fe-uptake genes are not fully understood. The C940-fe chlorotic melon (Cucumis melo) mutant known as fefe is unable to upregulate Fe-uptake genes, however, the FeFe gene had not been identified. In this study, we used two F2 mapping populations to map and identify the FeFe gene as bHLH38, a homolog of subgroup Ib bHLH genes from Arabidopsis thaliana that are involved in transcriptional regulation of Fe-uptake genes in partnership with the FIT gene...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28644710/study-of-artemisinin-and-sugars-accumulation-in-artemisia-vulgaris-and-artemisia-dracunculus-hairy-root-cultures
#14
Kateryna O Drobot, Nadiia A Matvieieva, Andriy M Ostapchuk, Maxim A Kharkhota
We studied the effect of genetic transformation on biologically active compounds (artemisinin and its co-products (ART) as well as sugars) accumulation in Artemisia vulgaris and A.dracunculus "hairy" root cultures. Glucose, fructose, sucrose and mannitol were accumulated in A. vulgaris and A. dracunculus "hairy" root lines. Genetic transformation has led in some cases to the sugars content increasing or appearing of non-relevant for the control plants carbohydrates. Sucrose content was 1.6 times higher in A...
June 23, 2017: Preparative Biochemistry & Biotechnology
https://www.readbyqxmd.com/read/28633497/genome-wide-identification-and-functional-characterization-of-udp-glucosyltransferase-genes-involved-in-flavonoid-biosynthesis-in-glycine-max
#15
Qinggang Yin, Guoan Shen, Shaokang Di, Cunying Fan, Zhenzhan Chang, Yongzhen Pang
Flavonoids, natural products abundant in the model legume Glycine max, confer benefits to plants and animals health. Flavonoids are present in soybean mainly as glycoconjugates. However, the biosynthesis mechanisms of flavonoid glycosides are largely unknown in G. max. In the present study, 212 putative UDP-glycosyltransferase (UGTs) genes were identified in G. max by genome-wide searching. The GmUGT genes were distributed differentially among the twenty chromosomes, and they were expressed in various tissues with distinct expression profile...
June 19, 2017: Plant & Cell Physiology
https://www.readbyqxmd.com/read/28592666/root-determined-nodulation1-is-required-for-m-truncatula-cle12-but-not-cle13-peptide-signaling-through-the-sunn-receptor-kinase
#16
Tessema Kassaw, Stephen Nowak, Elise Schnabel, Julia A Frugoli
The combinatorial interaction of a receptor kinase and a modified CLE peptide is involved in several developmental processes in plants, including Autoregulation of Nodulation (AON), which allows legumes to limit the number of root nodules formed based on available nitrogen and previous rhizobial colonization. Evidence supports modification of CLE peptides by enzymes of the hydroxyproline O-arabinosyltransferase (HPAT/RDN) family. Here we show by grafting and genetic analysis that in the AON pathway, RDN1, functioning in the root, acts upstream of the receptor kinase SUNN, functioning in the shoot...
June 7, 2017: Plant Physiology
https://www.readbyqxmd.com/read/28587128/yeast-extract-stimulates-ginsenoside-production-in-hairy-root-cultures-of-american-ginseng-cultivated-in-shake-flasks-and-nutrient-sprinkle-bioreactors
#17
Ewa Kochan, Piotr Szymczyk, Łukasz Kuźma, Anna Lipert, Grażyna Szymańska
One of the most effective strategies to enhance metabolite biosynthesis and accumulation in biotechnological systems is the use of elicitation processes. This study assesses the influence of different concentrations of yeast extract (YE) on ginsenoside biosynthesis in Panax quinquefolium (American ginseng) hairy roots cultivated in shake flasks and in a nutrient sprinkle bioreactor after 3 and 7 days of elicitation. The saponin content was determined using HPLC. The maximum yield (20 mg g(-1) d.w.) of the sum of six examined ginsenosides (Rb1, Rb2, Rc, Rd, Re and Rg1) in hairy roots cultivated in shake flasks was achieved after application of YE at 50 mg L(-1) concentration and 3 day exposure time...
May 26, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28567046/transcriptome-analysis-of-salicylic-acid-treatment-in-rehmannia-glutinosa-hairy-roots-using-rna-seq-technique-for-identification-of-genes-involved-in-acteoside-biosynthesis
#18
Fengqing Wang, Jingyu Zhi, Zhongyi Zhang, Lina Wang, Yanfei Suo, Caixia Xie, Mingjie Li, Bao Zhang, Jiafang Du, Li Gu, Hongzheng Sun
Rehmannia glutinosa is a common bulk medicinal material that has been widely used in China due to its active ingredients. Acteoside, one of the ingredients, has antioxidant, antinephritic, anti-inflammatory, hepatoprotective, immunomodulatory, and neuroprotective effects, is usually selected as a quality-control component for R. glutinosa herb in the Chinese Pharmacopeia. The acteoside biosynthesis pathway in R. glutinosa has not yet been clearly established. Herein, we describe the establishment of a genetic transformation system for R...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28559900/isoflavone-malonyltransferases-gmimat1-and-gmimat3-differently-modify-isoflavone-glucosides-in-soybean-glycine-max-under-various-stresses
#19
Muhammad Z Ahmad, Penghui Li, Junjie Wang, Naveed Ur Rehman, Jian Zhao
Malonylated isoflavones are the major forms of isoflavonoids in soybean plants, the genes responsible for their biosyntheses are not well understood, nor their physiological functions. Here we report a new benzylalcohol O-acetyltransferase, anthocyanin O-hydroxycinnamoyltransferase, anthranilate N-hydroxycinnamoyl/benzoyltransferase, deacetylvindoline 4-O-acetyltransferase (BAHD) family isoflavone glucoside malonyltransferase GmIMaT1, and GmIMaT3, which is allelic to the previously characterized GmMT7 and GmIF7MaT...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28554842/jasmonate-induced-biosynthesis-of-steroidal-glycoalkaloids-depends-on-coi1-proteins-in-tomato
#20
Ayman Abdelkareem, Chonprakun Thagun, Masaru Nakayasu, Masaharu Mizutani, Takashi Hashimoto, Tsubasa Shoji
In tomato, perception of jasmonates by a receptor complex, which includes the F-box protein CORONATINE INSENSITIVE 1 (COI1), elicits biosynthesis of defensive steroidal glycoalkaloids (SGAs) via a jasmonate-responsive ERF transcription factor, JRE4/GAME9. Although JRE4 is upregulated by jasmonate and induces the expression of many metabolic genes involved in SGA biosynthesis, it is not known whether JRE4 alone is sufficient for increased SGA biosynthesis upon activation of jasmonate signaling. Here, we show that application of methyl jasmonate induces the expression of JRE4 and SGA biosynthesis genes in leaves and hairy roots of wild-type tomato, but not in jasmonic acid insensitive 1 (jai1), a loss-of-function mutant allele of the tomato COI1 gene...
May 26, 2017: Biochemical and Biophysical Research Communications
keyword
keyword
74302
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"