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Amiloride

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https://www.readbyqxmd.com/read/29663016/gallic-acid-a-phenolic-compound-isolated-from-mimosa-bimucronata-dc-kuntze-leaves-induces-diuresis-and-saluresis-in-rats
#1
Fabile Schlickmann, Thaise Boeing, Luisa Nathália Bolda Mariano, Rita de Cássia Melo Vilhena de Andrade Fonseca da Silva, Luisa Mota da Silva, Sérgio Faloni de Andrade, Priscila de Souza, Valdir Cechinel-Filho
Although present in the leaves of Mimosa bimucronata (DC.) and many other medicinal plants commonly used to augment urinary volume excretion, the effects of gallic acid as a diuretic agent remain to be studied. Wistar rats were orally treated with vehicle, hydrochlorothiazide, or gallic acid. The effects of gallic acid in the presence of hydrochlorothiazide, furosemide, amiloride, L-NAME, atropine, and indomethacin were also investigated. Diuretic index, pH, conductivity, and electrolyte excretion were evaluated at the end of the experiment (after 8 or 24 h)...
April 16, 2018: Naunyn-Schmiedeberg's Archives of Pharmacology
https://www.readbyqxmd.com/read/29655877/endocrine-and-haemodynamic-changes-in-resistant-hypertension-and-blood-pressure-responses-to-spironolactone-or-amiloride-the-pathway-2-mechanisms-substudies
#2
Bryan Williams, Thomas M MacDonald, Steve V Morant, David J Webb, Peter Sever, Gordon T McInnes, Ian Ford, J Kennedy Cruickshank, Mark J Caulfield, Sandosh Padmanabhan, Isla S Mackenzie, Jackie Salsbury, Morris J Brown
BACKGROUND: In the PATHWAY-2 study of resistant hypertension, spironolactone reduced blood pressure substantially more than conventional antihypertensive drugs. We did three substudies to assess the mechanisms underlying this superiority and the pathogenesis of resistant hypertension. METHODS: PATHWAY-2 was a randomised, double-blind crossover trial done at 14 UK primary and secondary care sites in 314 patients with resistant hypertension. Patients were given 12 weeks of once daily treatment with each of placebo, spironolactone 25-50 mg, bisoprolol 5-10 mg, and doxazosin 4-8 mg and the change in home systolic blood pressure was assessed as the primary outcome...
April 11, 2018: Lancet Diabetes & Endocrinology
https://www.readbyqxmd.com/read/29617678/increased-asics-expression-via-the-camkii-creb-pathway-in-a-novel-mouse-model-of-trigeminal-pain
#3
Yan Wang, Xiujuan Fu, Lifang Huang, Xi Wang, Zuneng Lu, Fan Zhu, Zheman Xiao
BACKGROUND/AIMS: Migraine is a disabling condition that severely impacts socioeconomic function and quality of life. The focus of this study was to develop a mouse model of trigeminal pain that mimics migraine. METHODS: After undergoing dural cannulation surgery, mice were treated with repeated dural doses of an acidic solution to induce trigeminal pain. RESULTS: The method elicited intermittent, head-directed wiping and scratching as well as the expression of both the c-FOS gene in the spinal trigeminal nucleus caudalis and calcitonin gene related peptide (CGRP) in the periaqueductal grey matter...
March 28, 2018: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/29606130/proteasome-inhibitors-modulate-anticancer-and-anti-proliferative-properties-via-nf-kb-signaling-and-ubiquitin-proteasome-pathways-in-cancer-cell-lines-of-different-organs
#4
Asaf A Qureshi, Eleanor G Zuvanich, Dilshad A Khan, Shahida Mushtaq, Neerupma Silswal, Nilofer Qureshi
BACKGROUND: Cancer is second most common cause of death in the United State. There are over 100 different types of cancer associated with different human organs, predominantly breast, liver, pancreas, prostate, colon, rectum, lung, and stomach. We have recently reported properties of pro-inflammatory (for treatment of various types of cancers), and anti-inflammatory (for cardiovascular disease and diabetes) compounds. The major problem associated with development of anticancer drugs is their lack of solubility in aqueous solutions and severe side effects in cancer patients...
April 2, 2018: Lipids in Health and Disease
https://www.readbyqxmd.com/read/29601970/absorption-of-bicarbonate-in-sheep-omasum
#5
Driton Caushi, Holger Martens
Transport of bicarbonate across the isolated epithelium of sheep omasum was studied in vitro in Ussing chambers in combination with the pH-Stat method. The transport of HCO3 - occurred in both directions, but Jms HCO3 - was significant larger than Jsm . Reducing the activity of the apical Na/H exchanger by a low mucosal Na concentration caused a significant reduction of Jms HCO3 - . Mucosal amiloride or short chain fatty acids (25 mmol l-1 SCFA) numerically decreased Jms HCO3 - , but their combination (amiloride + SCFA) caused a significant reduction, which was also observed after addition of the carboanhydrase inhibitor ethoxyzolamide...
March 13, 2018: Research in Veterinary Science
https://www.readbyqxmd.com/read/29580888/characterization-of-mouse-chorda-tympani-responses-evoked-by-stimulation-of-anterior-or-posterior-fungiform-taste-papillae
#6
Stuart A McCaughey
Different gustatory papilla types vary in their locations on the tongue. Distinctions have often made between types, but variation within fungiform papillae has seldom been explored. Here, regional differences in fungiform papillae were investigated by flowing solutions selectively over either an anterior fungiform (AF, tongue tip) or a posterior fungiform (PF, middle third) region as taste-evoked activity was measured in the chorda tympani nerve of C57BL/6J (B6) mice. Significantly larger responses were evoked by NaCl applied to the AF than PF region, and the ENaC blocker amiloride reduced the NaCl response size only for the former...
March 23, 2018: Neuroscience Research
https://www.readbyqxmd.com/read/29580127/epithelial-sodium-channel-blockade-and-new-%C3%AE-enac-polymorphisms-among-normotensive-and-hypertensive-adult-nigerians
#7
Simiat O Elias, Olusoga A Sofola, Smith I Jaja
We sought to determine the effect of amiloride on blood pressure (BP) and the presence of polymorphisms of the β-subunit of the epithelial sodium channel (ENaC) among normotensive (NT) and hypertensive (HT) Nigerians. Healthy volunteers-47 NT and 53 age-matched HT were recruited after giving informed consent. Subjects were salt-loaded with 200 mmol of NaCl daily for 5 days. Following a week washout period, salt-loading was repeated in addition to the administration of 5 mg amiloride daily for five days. Blood pressure, plasma and urine electrolytes were measured at baseline, after salt-loading and after salt-loading plus amiloride...
March 26, 2018: Clinical and Experimental Hypertension: CHE
https://www.readbyqxmd.com/read/29576886/a-localized-surface-plasmon-resonance-sensing-method-for-simultaneous-determination-of-atenolol-and-amiloride-in-pharmaceutical-dosage-forms-and-urine-samples
#8
Marwa R El-Zahry
This contribution describes a simple, fast, and sensitive application of localized surface plasmon resonance effect of silver nanoparticles for simultaneous determination of antihypertensive drugs' mixture atenolol and amiloride in both pharmaceutical dosage forms and in biological samples (urine). Silver nanoparticles were prepared by chemical reduction of silver nitrate using hydroxylamine HCL in an alkaline medium. Application of silver-hydroxylamine nanoparticles (SH NPs) provides many advantages including reproducibility, sensitivity, and cost effective way of analyte determination...
2018: Journal of Analytical Methods in Chemistry
https://www.readbyqxmd.com/read/29558036/comparison-of-the-anticonvulsant-potency-of-various-diuretic-drugs-in-the-maximal-electroshock-induced-seizure-threshold-test-in-mice
#9
Katarzyna Załuska, Maria W Kondrat-Wróbel, Jarogniew J Łuszczki
BACKGROUND: The coexistence of seizures and arterial hypertension requires an adequate and efficacious treatment involving both protection from seizures and reduction of high arterial blood pressure. Accumulating evidence indicates that some diuretic drugs (with a well-established position in the treatment of arterial hypertension) also possess anticonvulsant properties in various experimental models of epilepsy. OBJECTIVES: The aim of this study was to assess the anticonvulsant potency of 6 commonly used diuretic drugs (i...
March 20, 2018: Advances in Clinical and Experimental Medicine: Official Organ Wroclaw Medical University
https://www.readbyqxmd.com/read/29555470/acute-stress-enhances-learning-and-memory-by-activating-acid-sensing-ion-channels-in-rats
#10
Shunjie Ye, Rong Yang, Qiuju Xiong, Youhua Yang, Lianying Zhou, Yeli Gong, Changlei Li, Zhenhan Ding, Guohai Ye, Zhe Xiong
Acute stress has been shown to enhance learning and memory ability, predominantly through the action of corticosteroid stress hormones. However, the valuable targets for promoting learning and memory induced by acute stress and the underlying molecular mechanisms remain unclear. Acid-sensing ion channels (ASICs) play an important role in central neuronal systems and involves in depression, synaptic plasticity and learning and memory. In the current study, we used a combination of electrophysiological and behavioral approaches in an effort to explore the effects of acute stress on ASICs...
April 15, 2018: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29534496/liddle-syndrome-review-of-the-literature-and-description-of-a-new-case
#11
REVIEW
Martina Tetti, Silvia Monticone, Jacopo Burrello, Patrizia Matarazzo, Franco Veglio, Barbara Pasini, Xavier Jeunemaitre, Paolo Mulatero
Liddle syndrome is an inherited form of low-renin hypertension, transmitted with an autosomal dominant pattern. The molecular basis of Liddle syndrome resides in germline mutations of the SCNN1A , SCNN1B and SCNN1G genes, encoding the α, β, and γ-subunits of the epithelial Na⁺ channel (ENaC), respectively. To date, 31 different causative mutations have been reported in 72 families from four continents. The majority of the substitutions cause an increased expression of the channel at the distal nephron apical membrane, with subsequent enhanced renal sodium reabsorption...
March 11, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29510579/amiloride-promotes-oligodendrocyte-survival-and-remyelination-after-spinal-cord-injury-in-rats
#12
Takeshi Imai, Hiroyuki Katoh, Kaori Suyama, Masahiro Kuroiwa, Sho Yanagisawa, Masahiko Watanabe
After spinal cord injury (SCI), secondary injury results in an expanding area of glial cell apoptosis. Oligodendrocyte precursor cells (OPCs) actively proliferate after SCI, but many of these cells undergo apoptosis. One of the factors that exacerbates secondary injury is endoplasmic reticulum (ER) stress. In this study, we tested the effects of amiloride treatment on the fate of OPCs during secondary injury in rats. Amiloride is an FDA-approved diuretic for treating hypertension, which in rats enhances ER stress response and suppresses the apoptosis of glial cells after SCI...
March 5, 2018: Journal of Clinical Medicine
https://www.readbyqxmd.com/read/29479446/use-of-acetazolamide-in-lithium-induced-nephrogenic-diabetes-insipidus-a-case-report
#13
Ricardo A Macau, Tiago Nunes da Silva, Joana Rego Silva, Ana Gonçalves Ferreira, Pedro Bravo
Lithium-induced nephrogenic diabetes insipidus (Li-NDI) is a rare and difficult-to-treat condition. A study in mice and two recent papers describe the use of acetazolamide in Li-NDI in 7 patients (a case report and a 6 patient series). We describe the case of a 63-year-old woman with bipolar disorder treated with lithium and no previous history of diabetes insipidus. She was hospitalized due to a bowel obstruction and developed severe dehydration after surgery when she was water deprived. After desmopressin administration and unsuccessful thiazide and amiloride treatment, acetazolamide was administrated to control polyuria and hydroelectrolytic disorders without significant side effects...
2018: Endocrinology, Diabetes & Metabolism Case Reports
https://www.readbyqxmd.com/read/29443544/taste-sensitivity-to-a-mixture-of-monosodium-glutamate-and-inosine-5-monophosphate-by-mice-lacking-both-subunits-of-the-t1r1-t1r3-amino-acid-receptor
#14
Ginger D Blonde, Susan P Travers, Alan Craig Spector
The taste of L-glutamate and its synergism with 5'-ribonucleotides is thought to be primarily mediated through the T1R1+T1R3 heterodimer in some mammals including rodents and humans. While knockout (KO) mice lacking either receptor subunit show impaired sensitivity to a range of monosodium glutamate (MSG) concentrations mixed with 2.5mM inosine 5'-monophosphate (IMP) in amiloride, wild-type (WT) controls can detect this IMP concentration, hindering direct comparison between genotypes. Moreover, some residual sensitivity persists in the KO group, suggesting that the remaining subunit could maintain a limited degree of function...
February 14, 2018: American Journal of Physiology. Regulatory, Integrative and Comparative Physiology
https://www.readbyqxmd.com/read/29433578/resistant-hypertension-on-treatment-reshypot-sequential-nephron-blockade-compared-to-dual-blockade-of-the-renin-angiotensin-aldosterone-system-plus-bisoprolol-in-the-treatment-of-resistant-arterial-hypertension-study-protocol-for-a-randomized-controlled-trial
#15
Elizabeth do Espirito Santo Cestário, Letícia Aparecida Barufi Fernandes, Luiz Tadeu Giollo-Júnior, Jéssica Rodrigues Roma Uyemura, Camila Suemi Sato Matarucco, Manoel Idelfonso Paz Landim, Luciana Neves Cosenso-Martin, Lúcia Helena Bonalume Tácito, Heitor Moreno, José Fernando Vilela-Martin, Juan Carlos Yugar-Toledo
BACKGROUND: Resistant hypertension is characterized when the blood pressure (BP) remains above the recommended goal after taking three antihypertensive drugs with synergistic actions at their maximum recommended tolerated doses, preferably including a diuretic. Identifying the contribution of intravascular volume and serum renin in maintaining BP levels could help tailor more effective hypertension treatment, whether acting on the control of intravascular volume or sodium balance, or acting on the effects of the renin-angiotensin-aldosterone system (RAAS) on the kidney...
February 12, 2018: Trials
https://www.readbyqxmd.com/read/29422286/visit-to-visit-lipid-variability-clinical-significance-effects-of-lipid-lowering-treatment-and-pharmaco-genetics
#16
REVIEW
Roelof A J Smit, Johan Wouter Jukema, Iris Postmus, Ian Ford, Pieternella Eline Slagboom, Bastiaan T Heijmans, Saskia Le Cessie, Stella Trompet
In recent years, visit-to-visit variability of serum lipids has been linked to both clinical outcomes and surrogate markers for vascular disease. In this article, we present an overview of the current evidence connecting this intraindividual variability to these outcome measures, discuss its interplay with lipid-lowering treatment, and describe the literature regarding genetic factors of possible interest. In addition, we undertook an explorative genome-wide association analysis on visit-to-visit variability of low-density lipoprotein cholesterol and high-density lipoprotein cholesterol, examining additive effects in 2530 participants from the placebo arm of the PROspective Study of Pravastatin in the Elderly at Risk trial...
March 2018: Journal of Clinical Lipidology
https://www.readbyqxmd.com/read/29413762/the-epithelial-sodium-channel-%C3%AE-enac-is-a-downstream-therapeutic-target-of-ascl1-in-pulmonary-neuroendocrine-tumors
#17
Min He, Shanshan Liu, Sachith Gallolu Kankanamalage, Mark D Borromeo, Luc Girard, Adi F Gazdar, John D Minna, Jane E Johnson, Melanie H Cobb
Small cell lung cancer (SCLC) is an aggressive neuroendocrine carcinoma, designated as a recalcitrant cancer by the National Cancer Institute, in urgent need of new rational therapeutic targets. Previous studies have determined that the basic helix-loop-helix transcription factor achaete-scute homolog 1 (ASCL1) is essential for the survival and progression of a fraction of pulmonary neuroendocrine cancer cells, which include both SCLC and a subset of non-SCLC. Previously, to understand how ASCL1 initiates tumorigenesis in pulmonary neuroendocrine cancer and identify the transcriptional targets of ASCL1, whole-genome RNA-sequencing analysis combined with chromatin immunoprecipitation-sequencing was performed with a series of lung cancer cell lines...
February 1, 2018: Translational Oncology
https://www.readbyqxmd.com/read/29404051/simultaneous-quantification-of-amiloride-and-hydrochlorothiazide-in-human-plasma-by-liquid-chromatography-tandem-mass-spectrometry
#18
Jaivik V Shah, Priyanka A Shah, Mallika Sanyal, Pranav S Shrivastav
A selective, sensitive and precise assay based on solid phase extraction and liquid chromatography-tandem mass spectrometry (LC-MS/MS) was developed for the simultaneous determination of amiloride (AMI) and hydrochlorothiazide (HCTZ) in human plasma. Sample clean-up with 250 µL of plasma was done on Phenomenex Strata™-X extraction cartridges using their labeled internal standards (AMI-15N3 and HCTZ-13C,d2). Chromatography was performed on Hypersil Gold C18 (50 mm×3.0 mm, 5 µm) column using acetonitrile with 4...
October 2017: Journal of Pharmaceutical Analysis
https://www.readbyqxmd.com/read/29397423/the-phosphorylation-site-t613-in-the-%C3%AE-subunit-of-rat-epithelial-na-channel-enac-modulates-channel-inhibition-by-nedd4-2
#19
Bettina Krueger, Limin Yang, Christoph Korbmacher, Robert Rauh
The epithelial Na+ channel (ENaC) is a heteromeric channel composed of three subunits (α, β, γ). At the C-terminus of each subunit, a PY-motif allows binding of the ubiquitin ligase Nedd4-2 which plays a key role in promoting ENaC retrieval from the plasma membrane. Phosphorylation of Nedd4-2 by the serum and glucocorticoid-inducible kinase 1 (Sgk1) reduces Nedd4-2 binding to the PY-motifs. In β and γENaC, threonine residues (βT613, γT623) belong to an extracellular signal-regulated kinase (ERK) motif and directly precede the PY-motifs...
February 3, 2018: Pflügers Archiv: European Journal of Physiology
https://www.readbyqxmd.com/read/29373035/epithelial-na-channel-enac-differentially-contributes-to-shear-stress-mediated-vascular-responsiveness-in-carotid-and-mesenteric-arteries-from-mice
#20
Zoe Ashley, Sama Mugloo, Fiona J McDonald, Martin Fronius
A potential "new player" in arteries for mediating shear stress responses is the Epithelial Sodium Channel (ENaC). ENaC's contribution as shear sensor in intact arteries, and particularly different types of arteries (conduit and resistance) is unknown. We investigated the role of ENaC in both conduit (carotid) and resistance (3rd order mesenteric) arteries isolated from C57Bl/6J mice. Vessel characteristics were determined at baseline (60 mmHg, no-flow), in response to increased intraluminal pressure and shear stress using a pressure myograph...
January 26, 2018: American Journal of Physiology. Heart and Circulatory Physiology
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