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https://www.readbyqxmd.com/read/28646803/effects-of-balneotherapy-on-serum-levels-of-shingosine-1-phosphate-in-patients-with-osteoarthritis
#1
Esra Aycan Ustyol, Fatih Karaarslan, Seldag Bekpinar, Kagan Ozkuk, Nergis Erdogan
Context • Balneotherapy is one of the most commonly used nonpharmacological interventions for osteoarthritis (OA), but its mechanism of action in relieving pain and stiffness and in improving physical function is not well understood. Studies have found that therapy provokes a series of neuroendocrinal reactions with anti-inflammatory and analgesic effects. Sphingosine-1-phosphate (S1P), a bioactive lipid, has been implicated as an important mediator in the maintenance of physiological processes (eg, vascular barrier integrity) and in pathophysiologic processes such as inflammatory conditions...
June 23, 2017: Alternative Therapies in Health and Medicine
https://www.readbyqxmd.com/read/28646774/observations-of-black-carbon-aerosols-characteristics-over-an-urban-environment-radiative-forcing-and-related-implications
#2
Samina Bibi, Khan Alam, Farrukh Chishtie, Humera Bibi, Said Rahman
With observations of black carbon (BC) aerosol concentrations, optical and radiative properties were obtained over the urban city of Karachi during the period of March 2006-December 2008. BC concentrations were continuously measured using an Aethalometer, while optical and radiative properties were estimated through the Optical Properties of Aerosols and Clouds (OPAC) and Santa Barbra DISORT Atmospheric Radiative Transfer (SBDART) models, respectively. For the study period, the measured BC concentrations were higher during January, February and November, while lower during May, June, July and August...
June 21, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28646683/fabrication-of-low-fire-hazard-flexible-poly-vinyl-chloride-via-reutilization-of-heavy-metal-biosorbents
#3
Ye-Tang Pan, De-Yi Wang
As a naturally abundant biopolymer, chitosan is considered to be a suitable adsorbent for stannate (SnO3(2-)) in tin plating wastewater. However, mass transfer of the adsorbent and its recycling remain challenging problems. Though flexible poly(vinyl chloride) (PVC) is highly flammable due to the addition of plasticizers, the traditional flame retardant, antimony trioxide (Sb2O3), is potentially harmful. In this study, chitosan was anchored onto the surface of PVC resin to adsorb SnO3(2-) from wastewater. Thereafter, tin-doped chitosan-coated PVC resin was readily recycled and processed into a flexible PVC composite (modified fPVC)...
June 15, 2017: Journal of Hazardous Materials
https://www.readbyqxmd.com/read/28646608/fluid-filling-of-the-digestive-tract-for-improved-proton-resonance-frequency-shift-based-mr-thermometry-in-the-pancreas
#4
Cyril J Ferrer, Lambertus W Bartels, Marijn van Stralen, Baudouin Denis de Senneville, Chrit T W Moonen, Clemens Bos
PURPOSE: To demonstrate that fluid filling of the digestive tract improves the performance of respiratory motion-compensated proton resonance frequency shift (PRFS)-based magnetic resonance (MR) thermometry in the pancreas. MATERIALS AND METHODS: In seven volunteers (without heating), we evaluated PRFS thermometry in the pancreas with and without filling of the surrounding digestive tract. All data acquisition was performed at 1.5T, then all datasets were analyzed and compared with three different PRFS respiratory motion-compensated thermometry methods: gating, multibaseline, and referenceless...
June 24, 2017: Journal of Magnetic Resonance Imaging: JMRI
https://www.readbyqxmd.com/read/28646532/acquired-bilateral-telangiectasia-macularis-eruptiva-perstans-a-unique-clinical-feature-of-photodamaging-rather-than-a-subtype-of-cutaneous-mastocytosis
#5
Hye-Rim Moon, Young Jae Kim, Joon Min Jung, Chong Hyun Won, Mi Woo Lee, Jee Ho Choi, Sung Eun Chang
Telangiectasia macularis eruptiva perstans (TMEP) is a rare subtype of cutaneous mastocytosis, characterized by telangiectatic tan to brown macules on the trunk and extremities. Although TMEP has been descried as an uncommon disease in the literature, we often encounter patients with TMEP lesions in the outpatient clinic. We aimed to assess the clinical and histopathological characteristics of acquired bilateral TMEP, and the pathophysiological mechanism of acquired bilateral TMEP among these patients. We retrospectively reviewed 30 patients (28 men and 2 women) with acquired bilateral TMEP; multiple telangiectatic dark red to brown macules that were symmetrically distributed...
June 23, 2017: Journal of Dermatology
https://www.readbyqxmd.com/read/28646266/pericytes-promote-abnormal-tumor-angiogenesis-in-a-rat-rg2-glioma-model
#6
Junji Hosono, Shunichi Morikawa, Taichi Ezaki, Takakazu Kawamata, Yoshikazu Okada
In glioma angiogenesis, tumor vessels cause morphological and functional abnormalities associated with malignancy and tumor progression. We hypothesized that certain structural changes or scantiness of functional pericytes may be involved in the formation of dysfunctional blood vessels in gliomas. In this study, we performed morphological examinations to elucidate the possible involvement of pericytes in brain tumor vessel abnormalities using a rat RG2 glioma model. After implantation of RG2 glioma cells in the syngeneic rat brain, gliomas were formed as early as day 7...
June 23, 2017: Brain Tumor Pathology
https://www.readbyqxmd.com/read/28646212/direct-observation-of-a-non-crystalline-state-of-li2s-p2s5-solid-electrolytes
#7
Hirofumi Tsukasaki, Shigeo Mori, Hideyuki Morimoto, Akitoshi Hayashi, Masahiro Tatsumisago
There are two types of solid electrolytes which has been recently expected to be applied to all-solid-state batteries. One is the glasses characterized by an amorphous state. The other is the glass ceramics containing crystalline in an amorphous matrix. However, the non-crystalline state of glasses and glass ceramics is still an open question. It has been anticipated that sea-island and core-shell structures including crystalline nanoparticles have been proposed as candidate models for glass ceramics. Nevertheless, no direct observation has been conducted so far...
June 23, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28646096/heat-stress-causes-dysfunctional-autophagy-in-oxidative-skeletal-muscle
#8
Alexandra J Brownstein, Shanthi Ganesan, Corey M Summers, Sarah Pearce, Benjamin J Hale, Jason W Ross, Nicholas Gabler, Jacob T Seibert, Robert P Rhoads, Lance H Baumgard, Joshua T Selsby
We have previously established that 24 h of environmental hyperthermia causes oxidative stress and have implicated mitochondria as likely contributors to this process. Given this, we hypothesized that heat stress would lead to increased autophagy/mitophagy and a reduction in mitochondrial content. To address this hypothesis pigs were housed in thermoneutral (TN; 20°C) or heat stress (35°C) conditions for 1- (HS1) or 3- (HS3) days and the red and white portions of the semitendinosus collected. We did not detect differences in glycolytic muscle...
June 2017: Physiological Reports
https://www.readbyqxmd.com/read/28646043/an-efficient-flp-based-toolkit-for-spatiotemporal-control-of-gene-expression-in-caenorhabditis-elegans
#9
Celia María Muñoz-Jiménez, Cristina Ayuso, Agnieszka Dobrzynska, Antonio Torres, Patricia de la Cruz Ruiz, Peter Askjaer
Site-specific recombinases are potent tools to regulate gene expression. In particular, the Cre and FLP enzymes are widely used to either activate or inactivate genes in a precise spatiotemporal manner. Both recombinases work efficiently in the popular model organism Caenorhabditis elegans but their use in this nematode is still only sporadic. To increase the utility of the FLP system in C. elegans we have generated a series of single-copy transgenic strains that stably express an optimized version of FLP in specific tissues or by heat induction...
June 23, 2017: Genetics
https://www.readbyqxmd.com/read/28645998/stability-of-tacrolimus-ophthalmic-solution
#10
Carlos Ezquer-Garin, Rafael Ferriols-Lisart, Manuel Alós-Almiñana
PURPOSE: The stability of 0.3-mg/mL tacrolimus ophthalmic solution at different storage temperatures was studied. METHODS: A sterile ophthalmic solution of 0.3 mg/mL tacrolimus was prepared in triplicate under aseptic conditions by diluting tacrolimus in eye drops. Three aliquots of this solution were transferred into polypropylene bottles and stored at 25, 2-8, or -15 to -25 °C. Samples were collected immediately after preparation and at selected time points and assayed in triplicate using high-performance liquid chromatography (HPLC)...
July 1, 2017: American Journal of Health-system Pharmacy: AJHP
https://www.readbyqxmd.com/read/28645849/thermal-management-in-closed-incubators-new-software-for-assessing-the-impact-of-humidity-on-the-optimal-incubator-air-temperature
#11
Stéphane Delanaud, Pauline Decima, Amandine Pelletier, Jean-Pierre Libert, Estelle Durand, Erwan Stephan-Blanchard, Véronique Bach, Pierre Tourneux
BACKGROUND: Low-birth-weight (LBW) neonates are nursed in closed incubators to prevent transcutaneous water loss. The RH's impact on the optimal incubator air temperature setting has not been studied. METHODS: On the basis of a clinical cohort study, we modelled all the ambient parameters influencing body heat losses and gains. The algorithm quantifies the change in RH on the air temperature, to maintain optimal thermal conditions in the incubator. RESULTS: Twenty-three neonates (gestational age (GA): 30...
June 20, 2017: Medical Engineering & Physics
https://www.readbyqxmd.com/read/28645771/dectin-1-expression-by-macrophages-and-related-antifungal-mechanisms-in-a-murine-model-of-sporothrix-schenckii-sensu-stricto-systemic-infection
#12
Juliana Aparecida Jellmayer, Lucas Souza Ferreira, Francine Alessandra Manente, Amanda Costa Gonçalves, Marisa Campos Polesi, Alexander Batista-Duharte, Iracilda Zeppone Carlos
The available information about the role of Dectin-1 in sporotrichosis is scarce. Hence, we aimed to assess Dectin-1 expression by macrophages and the activation of some related antifungal mechanisms during the Sporothrix schenckii sensu stricto infection as a first attempt to elucidate the role of this receptor in sporotrichosis. Balb/c mice were intraperitoneally infected with S. schenckii sensu stricto yeast ATCC 16345 and euthanized on days 5, 10 and 15 post-infection, when the following parameters were evaluated: fungal burden in spleen, Dectin-1 expression and nitric oxide (NO) production by peritoneal macrophages, as well as IL-1β, TNF-α and IL-10 ex vivo secretion by these same cells...
June 20, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/28645756/an-aptamer-based-pcr-method-coupled-with-magnetic-immunoseparation-for-sensitive-detection-of-salmonella-typhimurium-in-ground-turkey
#13
Lijun Wang, Ronghui Wang, Hong Wang, Michael Slavik, Hua Wei, Yanbin Li
Aptamers are single-stranded oligonucleotide ligands that can bind to targets with high affinity and specificity. They have been widely studied in the field of diagnostics as alternatives to antibodies due to their favorable features such as easy labeling, temperature tolerance, lower cost and recognition of a wide variety of targets. In this study, an aptamer-based PCR method coupled with magnetic immunoseparation was developed to detect S. Typhimurium from ground turkey. Firstly, biotinylated polyclonal anti-S...
June 20, 2017: Analytical Biochemistry
https://www.readbyqxmd.com/read/28645718/optimizing-adjuvants-for-intradermal-delivery-of-menc-glycoconjugate-vaccine
#14
Agnese Donadei, Cristiana Balocchi, Maria R Romano, Luigi Panza, Roberto Adamo, Francesco Berti, Derek T O'Hagan, Simona Gallorini, Barbara C Baudner
Intradermal vaccine delivery is a promising alternative to the conventional intramuscular route. The skin layer is immunologically supported by a densely network of antigen presenting cells, while the skeletal muscle is loaded with a relatively sparse population of APCs. Nevertheless, the vaccine to be suitable for intradermal delivery needs a new formulation to facilitate either smaller injection volumes or the introduction into new delivery devises as micro-needles. This study presents a proof of concept for intradermal delivery of the MenC-CRM197 glycoconjugate vaccine using a mouse model...
June 20, 2017: Vaccine
https://www.readbyqxmd.com/read/28645570/quantitative-proteomics-analysis-by-itraq-revealed-underlying-changes-in-thermotolerance-of-arthrospira-platensis
#15
Rong Chang, Bingxin Lv, Bosheng Li
Growth temperature is a critical factor that affects cultivation of Arthrospira platensis which is a type of cyanobacterium widely known as Spirulina that has significant commercial value. To investigate the molecular mechanism underlying the thermotolerance of Spirulina, differential protein expression profiling was carried out using iTRAQ-based quantitative proteomic analysis. This study only analyzed changes in thylakoids. Among the 2085 proteins quantified, 43 differentially expressed proteins were selected based on the fold change cutoff scores of ≥2 or ≤0...
June 20, 2017: Journal of Proteomics
https://www.readbyqxmd.com/read/28645179/system-wide-analysis-of-short-term-response-to-high-temperature-in-pinus-radiata
#16
Mónica Escandón, Luis Valledor, Jesús Pascual, Gloria Pinto, María Jesús Cañal, Mónica Meijón
Pinus radiata seedlings, the most widely planted pine species in the world, were exposed to temperatures within a range mimicking future scenarios based on current models of heat increase. The short-term heat response in P. radiata was studied in detail by exploring the metabolome, proteome and targeted transcriptome. The use of complementary mass spectrometry techniques, GC-MS and LC-Orbitrap-MS, together with novel bioinformatics tools allowed the reliable quantification of 2,075 metabolites and 901 protein groups...
June 22, 2017: Journal of Experimental Botany
https://www.readbyqxmd.com/read/28644990/global-transcriptional-response-of-escherichia-coli-mg1655-cells-exposed-to-the-oxygenated-monoterpenes-citral-and-carvacrol
#17
Beatriz Chueca, Elisa Pérez-Sáez, Rafael Pagán, Diego García-Gonzalo
DNA microarrays were used to study the mechanism of bacterial inactivation by carvacrol and citral. After 10-min treatments of Escherichia coli MG1655 cells with 100 and 50ppm of carvacrol and citral, 76 and 156 genes demonstrated significant transcriptional differences (p≤0.05), respectively. Among the up-regulated genes after carvacrol treatment, we found gene coding for multidrug efflux pumps (acrA, mdtM), genes related to phage shock response (pspA, pspB, pspC, pspD, pspF and pspG), biosynthesis of arginine (argC, argG, artJ), and purine nucleotides (purC, purM)...
June 20, 2017: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/28644983/future-heat-waves-due-to-climate-change-threaten-the-survival-of-p-%C3%A2-oceanica-seedlings
#18
Laura Guerrero-Meseguer, Arnaldo Marín, Carlos Sanz-Lázaro
Extreme weather events are major drivers of ecological change, and their occurrence is likely to increase due to climate change. The transient increases in atmospheric temperatures are leading to a greater occurrence of heat waves, extreme events that can produce a substantial warming of water, especially in enclosed basins such as the Mediterranean Sea. Here, we tested the effects of current and predicted heat waves on the early stages of development of the seagrass Posidonia oceanica. Temperatures above 27 °C limited the growth of the plant by inhibiting its photosynthetic system...
June 20, 2017: Environmental Pollution
https://www.readbyqxmd.com/read/28644899/new-insights-in-dehydration-stress-behavior-of-two-maize-hybrids-using-advanced-distributed-reactivity-model-drm-responses-to-the-impact-of-24-epibrassinolide
#19
Hadi Waisi, Bojan Janković, Marija Janković, Bogdan Nikolić, Ivica Dimkić, Blažo Lalević, Vera Raičević
Proposed distributed reactivity model of dehydration for seedling parts of two various maize hybrids (ZP434, ZP704) was established. Dehydration stresses were induced thermally, which is also accompanied by response of hybrids to heat stress. It was found that an increased value of activation energy counterparts within radicle dehydration of ZP434, with a high concentration of 24-epibrassinolide (24-EBL) at elevated operating temperatures, probably causes activation of diffusion mechanisms in cutin network and may increases likelihood of formation of free volumes, large enough to accommodate diffusing molecule...
2017: PloS One
https://www.readbyqxmd.com/read/28644025/a-mitochondria-targeted-cryptocyanine-based-photothermogenic-photosensitizer
#20
Hyo Sung Jung, Jae Hong Lee, Kyutae Kim, Seyoung Koo, Peter Verwilst, Jonathan L Sessler, Chulhun Kang, Jong Seung Kim
Cryptocyanine-based probes exhibit highly efficient photothermal conversion and represent a new class of photothermal agents for use in photothermal therapy (PTT). With the thermal susceptibility of mitochondria in mind, we have prepared a mitochondria-targeted, NIR-absorbing cryptocyanine probe (Mito-CCy) and evaluated its photophysical properties, photothermal conversion efficiency, biological compatibility, cytotoxicity, and mitochondrial localization in HeLa cells. Upon subjecting 0.5 mL of a PBS buffer solution (10 mM, pH 7...
June 23, 2017: Journal of the American Chemical Society
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