keyword
MENU ▼
Read by QxMD icon Read
search

phosphatidylserine, brain

keyword
https://www.readbyqxmd.com/read/29673196/annexins-in-glaucoma
#1
REVIEW
Timothy E Yap, Benjamin Michael Davis, Li Guo, Eduardo M Normando, Maria Francesca Cordeiro
Glaucoma is one of the leading causes of irreversible visual loss, which has been estimated to affect 3.5% of those over 40 years old and projected to affect a total of 112 million people by 2040. Such a dramatic increase in affected patients demonstrates the need for continual improvement in the way we diagnose and treat this condition. Annexin A5 is a 36 kDa protein that is ubiquitously expressed in humans and is studied as an indicator of apoptosis in several fields. This molecule has a high calcium-dependent affinity for phosphatidylserine, a cell membrane phospholipid externalized to the outer cell membrane in early apoptosis...
April 17, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29567363/screening-of-stationary-phase-selectivities-for-global-lipid-profiling-by-ultrahigh-performance-supercritical-fluid-chromatography
#2
Said Al Hamimi, Margareta Sandahl, Marina Armeni, Charlotta Turner, Peter Spégel
The performance of seven sub-2-μm particle packed columns (2-picolylamine, 2-PIC; charged surface hybrid fluoro-phenyl, CSH-FP; high strength silica C18 SB, HSS-C18 ; diethylamine, DEA; 1-aminoanthracene, 1-AA; high density diol and ethylene bridged hybrid; BEH) was examined for lipid separation in ultra-high performance supercritical fluid chromatography (UHPSFC) coupled to quadrupole time-of-flight mass spectrometry. Based on the results of the column screening a method for profiling of multiple lipid species from the major lipid classes was developed...
March 14, 2018: Journal of Chromatography. A
https://www.readbyqxmd.com/read/29556135/-in-vitro-characterization-of-arylhydrazones-of-active-methylene-derivatives
#3
Suresh Palanivel, Anastasia Zhurina, Phuong Doan, Jerome G Chandraseelan, Vinoth Kumar Megraj Khandelwal, Fedor I Zubkov, Kamran T Mahmudov, Armando J L Pombeiro, Olli Yli-Harja, Meenakshisundaram Kandhavelu
Arylhydrazones of active methylene compounds (AHAMCs) are potent chemotherapy agents for the cancer treatment. AHAMCs enhance the apoptotic cell death and antiproliferation properties in cancer cells. In this study, a series of AHAMCs, 13 compounds, was assayed for cytotoxicity, apoptosis, externalization of phosphatidylserine, heterogeneity and cellular calcium level changes. The in vitro cytotoxicity study against HEK293T cells suggests that AHAMCs have significant cytotoxic effect over the concentrations...
March 2018: Saudi Pharmaceutical Journal: SPJ: the Official Publication of the Saudi Pharmaceutical Society
https://www.readbyqxmd.com/read/29543435/a%C3%AE-42-protofibrils-interact-with-and-are-trafficked-through-microglial-derived-microvesicles
#4
Lisa K Gouwens, Mudar S Ismail, Victoria A Rogers, Nathan T Zeller, Evan C Garrad, Fatima S Amtashar, Nyasha J Makoni, David C Osborn, Michael R Nichols
Microvesicles (MVs) and exosomes comprise a class of cell-secreted particles termed extracellular vesicles (EVs). These cargo-holding vesicles mediate cell-to-cell communication and have recently been implicated in neurodegenerative diseases such as Alzheimer's disease (AD). The two types of EVs are distinguished by the mechanism of cell release and their size, with the smaller exosomes and the larger MVs ranging from 30 to 100 nm and 100 nm to 1 μm in diameter, respectively. MV numbers are increased in AD and appear to interact with amyloid-β peptide (Aβ), the primary protein component of the neuritic plaques in the AD brain...
March 15, 2018: ACS Chemical Neuroscience
https://www.readbyqxmd.com/read/29541056/lipidomics-analysis-of-behavioral-variant-frontotemporal-dementia-a-scope-for-biomarker-development
#5
Woojin Scott Kim, Eve Jary, Russell Pickford, Ying He, Rebekah M Ahmed, Olivier Piguet, John R Hodges, Glenda M Halliday
Behavioral variant frontotemporal dementia (bvFTD) is the most prevalent form of FTD syndromes. bvFTD is characterized clinically by changes in behavior and cognition and pathologically by focal brain atrophy and concomitant loss of lipids. bvFTD is further characterized by eating abnormalities that result in dyslipidemia. Although dyslipidemia is apparent in bvFTD, very little is known about global lipid changes in bvFTD and lipid dysregulation underlying bvFTD. Here, we undertook a comprehensive lipidomics analysis of blood plasma from patients with bvFTD, patients with Alzheimer's disease (AD) and controls, using liquid chromatography-tandem mass spectrometry, with the aim of understanding lipid dysregulation in bvFTD...
2018: Frontiers in Neurology
https://www.readbyqxmd.com/read/29470956/metabolomic-insights-into-the-effects-of-thyroid-hormone-on-rana-lithobates-catesbeiana-metamorphosis-using-whole-body-matrix-assisted-laser-desorption-ionization-mass-spectrometry-imaging-maldi-msi
#6
Teesha C Luehr, Emily M Koide, Xiaodong Wang, Jun Han, Christoph H Borchers, Caren C Helbing
Anuran metamorphosis involves the transformation of an aquatic tadpole into a juvenile frog. This process is completely dependent upon thyroid hormones (THs). Although much research has been focused on changes in gene expression programs during this postembryonic developmental period, transitions in the metabolic profiles are relatively poorly understood. Matrix Assisted Laser Desorption/Ionization-Mass Spectrometry Imaging (MALDI-MSI) is a technique that generates highly multiplexed mass spectra while retaining spatial location information on a thin tissue section...
February 19, 2018: General and Comparative Endocrinology
https://www.readbyqxmd.com/read/29469952/profiling-and-imaging-of-phospholipids-in-brains-of-abcd1-deficient-mice
#7
Kotaro Hama, Yuko Fujiwara, Masashi Morita, Fumiyoshi Yamazaki, Yuko Nakashima, Shiro Takei, Shigeo Takashima, Mitsutoshi Setou, Nobuyuki Shimozawa, Tsuneo Imanaka, Kazuaki Yokoyama
ABCD1 is a gene responsible for X-linked adrenoleukodystrophy (X-ALD), and is critical for the transport of very long-chain fatty acids (VLCFA) into peroxisomes and subsequent β-oxidation. VLCFA-containing lipids accumulate in X-ALD patients, although the effect of ABCD1-deficiency on each lipid species in the central nervous system has not been fully characterized. In this study, each phospholipid and lysophospholipid species in Abcd1-deficient mice brains were profiled by liquid chromatography-mass spectrometry...
January 2018: Lipids
https://www.readbyqxmd.com/read/29416059/layer-specific-lipid-signatures-in-the-human-subventricular-zone-demonstrated-by-imaging-mass-spectrometry
#8
Mandana Hunter, Nicholas J Demarais, Richard L M Faull, Angus C Grey, Maurice A Curtis
The subventricular zone is a key site of adult neurogenesis and is also implicated in neurodegenerative diseases and brain cancers. In the subventricular zone, cell proliferation, migration and differentiation of nascent stem cells and neuroblasts are regulated at least in part by lipids. The human subventricular zone is distinctly layered and each layer contains discrete cell types that support the processes of neuroblast migration and neurogenesis. We set out to determine the lipid signatures of each subventricular layer in the adult human brain (n = 4)...
February 7, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29353693/multiscale-molecular-dynamics-simulations-of-lipid-interactions-with-p-glycoprotein-in-a-complex-membrane
#9
Laura Domicevica, Heidi Koldsø, Philip C Biggin
P-glycoprotein (P-gp) can transport a wide range of very different hydrophobic organic molecules across the membrane. Its ability to extrude molecules from the cell creates delivery problems for drugs that target proteins in the central nervous system (CNS) and also causes drug-resistance in many forms of cancer. Whether a drug will be susceptible to export by P-gp is difficult to predict and currently this is usually assessed with empirical and/or animal models. Thus, there is a need to better understand how P-gp works at the molecular level in order to fulfil the 3Rs: Refinement, reduction and replacement of animals in research...
March 2018: Journal of Molecular Graphics & Modelling
https://www.readbyqxmd.com/read/29318974/-understanding-the-role-of-hypoxia-inducible-factor-during-neurodegeneration-for-new-therapeutics-opportunities
#10
Amalia Merelli, Julio Cesar Garcia Rodriguez, Jaume Folch, Marcelo R Regueiro, Antoni Camins, Alberto Lazarowski
Neurodegeneration (NDG) is linked with the progressive loss of neural function with intellectual and/or motor impairment. Several diseases affecting older individuals, including Alzheimer's disease, Amyotrophic Lateral Sclerosis, Huntington's disease, Parkinson's disease, stroke, Multiple Sclerosis and many others, are the most relevant disorders associated with NDG. Since other pathologies such as refractory epilepsy, brain infections, or hereditary diseases such as "neurodegeneration with brain iron accumulation", also lead to chronic brain inflammation with loss of neural cells, NDG can be said to affect all ages...
January 10, 2018: Current Neuropharmacology
https://www.readbyqxmd.com/read/29288365/methylphenidate-disrupts-cytoskeletal-homeostasis-and-reduces-membrane-associated-lipid-content-in-juvenile-rat-hippocampus
#11
Felipe Schmitz, Paula Pierozan, Helena Biasibetti-Brendler, Fernanda Silva Ferreira, Fernanda Dos Santos Petry, Vera Maria Treis Trindade, Regina Pessoa-Pureur, Angela T S Wyse
Although methylphenidate (MPH) is ubiquitously prescribed to children and adolescents, the consequences of chronic utilization of this psychostimulant are poorly understood. In this study, we investigated the effects of MPH on cytoskeletal homeostasis and lipid content in rat hippocampus. Wistar rats received intraperitoneal injections of MPH (2.0 mg/kg) or saline solution (controls), once a day, from the 15th to the 44th day of age. Results showed that MPH provoked hypophosphorylation of glial fibrillary acidic protein (GFAP) and reduced its immunocontent...
December 29, 2017: Metabolic Brain Disease
https://www.readbyqxmd.com/read/29251628/erythrocyte-efferocytosis-modulates-macrophages-towards-recovery-after-intracerebral-hemorrhage
#12
Che-Feng Chang, Brittany A Goods, Michael H Askenase, Matthew D Hammond, Stephen C Renfroe, Arthur F Steinschneider, Margaret J Landreneau, Youxi Ai, Hannah E Beatty, Luís Henrique Angenendt da Costa, Matthias Mack, Kevin N Sheth, David M Greer, Anita Huttner, Daniel Coman, Fahmeed Hyder, Sourav Ghosh, Carla V Rothlin, J Christopher Love, Lauren H Sansing
Macrophages are a source of both proinflammatory and restorative functions in damaged tissue through complex dynamic phenotypic changes. Here, we sought to determine whether monocyte-derived macrophages (MDMs) contribute to recovery after acute sterile brain injury. By profiling the transcriptional dynamics of MDMs in the murine brain after experimental intracerebral hemorrhage (ICH), we found robust phenotypic changes in the infiltrating MDMs over time and demonstrated that MDMs are essential for optimal hematoma clearance and neurological recovery...
February 1, 2018: Journal of Clinical Investigation
https://www.readbyqxmd.com/read/29244485/critical-role-of-trp-588-of-presynaptic-munc13-1-for-ligand-binding-and-membrane-translocation
#13
Joydip Das, Noemi Kedei, Jessica S Kelsey, Youngki You, Satyabrata Pany, Gary A Mitchell, Nancy E Lewin, Peter M Blumberg
Munc13-1 is a presynaptic active-zone protein essential for neurotransmitter release and presynaptic plasticity in the brain. This multidomain scaffold protein contains a C1 domain that binds to the activator diacylglycerol/phorbol ester. Although the C1 domain bears close structural homology with the C1 domains of protein kinase C (PKC), the tryptophan residue at position 22 (588 in the full-length Munc13-1) occludes the activator binding pocket, which is not the case for PKC. To elucidate the role of this tryptophan, we generated W22A, W22K, W22D, W22Y, and W22F substitutions in the full-length Munc13-1, expressed the GFP-tagged constructs in Neuro-2a cells, and measured their membrane translocation in response to phorbol ester treatment by imaging of the live cells using confocal microscopy...
February 6, 2018: Biochemistry
https://www.readbyqxmd.com/read/29162596/lactadherin-promotes-microvesicle-clearance-to-prevent-coagulopathy-and-improves-survival-of-severe-tbi-mice
#14
Yuan Zhou, Wei Cai, Zilong Zhao, Tristan Hilton, Min Wang, Jason Yeon, Wei Liu, Fangyi Zhang, Fu-Dong Shi, Xiaoping Wu, Perumal Thiagarajan, Min Li, Jianning Zhang, Jing-Fei Dong
Coagulopathy is common in patients with traumatic brain injury (TBI) and predicts poor clinical outcomes. We have shown that brain-derived extracellular microvesicles, including extracellular mitochondria, play a key role in the development of TBI-induced coagulopathy. Here, we further show in mouse models that the apoptotic cell-scavenging factor lactadherin, given at a single dose of 400 μg/kg 30 minutes before (preconditioning) or 30 minutes after cerebral fluid percussion injury, prevented coagulopathy as defined by clotting time, fibrinolysis, intravascular fibrin deposition, and microvascular bleeding of the lungs...
February 1, 2018: Blood
https://www.readbyqxmd.com/read/29152944/combined-oral-supplementation-of-chromium-picolinate-docosahexaenoic-acid-and-boron-enhances-neuroprotection-in-rats-fed-a-high-fat-diet
#15
Cemal Orhan, Nurhan Şahin, Zeynep Tuzcu, James R. Komorowski, Kazım Şahin
Background/aim: A novel complex of a nutritional supplement (CDB) contains chromium picolinate (CrPic), phosphatidylserine (PS), docosahexaenoic acid (DHA), and boron (B). The present study aimed to investigate the effects of CDB on the metabolic profile and memory acquisition in rats fed a high-fat diet (HFD). Materials and methods: Male Wistar rats were divided into six groups and received either a regular diet or HFD supplemented with or without different levels of CDB (0, 11, or 22 mg/kg BW). Results: Rats fed the HFD had greater glucose, insulin, lipid profile, and serum malondialdehyde concentrations, but lower serotonin and tryptophan in the serum and brain and lower Cr concentrations in serum, kidney, brain, and liver (P < 0...
November 13, 2017: Turkish Journal of Medical Sciences
https://www.readbyqxmd.com/read/29125686/neurophagy-the-phagocytosis-of-live-neurons-and-synapses-by-glia-contributes-to-brain-development-and-disease
#16
REVIEW
Anna Vilalta, Guy C Brown
It was previously thought that neurons were phagocytosed only when dead or dying. However, it is increasingly clear that viable synapses, dendrites, axons and whole neurons can be phagocytosed alive (defined here as neurophagy), and this may contribute to a wide range of developmental, physiological and pathological processes. Phagocytosis of live synapses, dendrites and axons by glia contributes to experience-dependent sculpting of neuronal networks during development, but excessive phagocytosis of synapses may contribute to pathology in Alzheimer's disease, schizophrenia and ageing...
November 10, 2017: FEBS Journal
https://www.readbyqxmd.com/read/29095596/1-6-diphenyl-1-3-5-hexatriene-dph-as-a-novel-matrix-for-maldi-ms-imaging-of-fatty-acids-phospholipids-and-sulfatides-in-brain-tissues
#17
Hanadi Ibrahim, Kristina Jurcic, Jasmine S-H Wang, Shawn N Whitehead, Ken K-C Yeung
1,6-Diphenyl-1,3,5-hexatriene (DPH) is a commonly used fluorescence probe for studying cell membrane-lipids due to its affinity toward the acyl chains in the phospholipid bilayers. In this work, we investigated its use in matrix-assisted laser desorption/ionization (MALDI) as a new matrix for mass spectrometry imaging (MSI) of mouse and rat brain tissue. DPH exhibits very minimal matrix-induced background signals for the analysis of small molecules (below m/z of 1000). In the negative ion mode, DPH permits the highly sensitive detection of small fatty acids (m/z 200-350) as well as a variety of large lipids up to m/z of 1000, including lyso-phospholipid, phosphatidic acid (PA), phosphoethanolamine (PE), phosphatidylserine (PS), phosphatidylglycerol (PG), phosphatidylinositol (PI), and sulfatides (ST)...
December 5, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/29069749/-in-vitro-and-in-vivo-cytotoxic-activity-of-human-lactoferricin-derived-antitumor-peptide-r-dim-p-lf11-334-on-human-malignant-melanoma
#18
Sabrina Riedl, Beate Rinner, Helmut Schaider, Bernadette Liegl-Atzwanger, Katharina Meditz, Julia Preishuber-Pflügl, Sarah Grissenberger, Karl Lohner, Dagmar Zweytick
Di-peptides derived from the human host defense peptide lactoferricin were previously described to specifically interact with the negatively charged lipid phosphatidylserine exposed by cancer cells. In this study one further derivative, namely R-DIM-P-LF11-334 is shown to exhibit even increased cancer toxicity in vitro and in vivo while non-neoplastic cells are not harmed. In liposomal model systems composed of phosphatidylserine mimicking cancerous and phosphatidylcholine mimicking non-cancerous membranes the specific interaction with the cancer marker PS was confirmed by specific induction of membrane perturbation and permeabilization in presence of the peptide...
September 22, 2017: Oncotarget
https://www.readbyqxmd.com/read/29058414/hydrophilic-interaction-liquid-chromatography-mass-spectrometry-characterization-of-gangliosides-in-biological-samples
#19
Roman Hájek, Robert Jirásko, Miroslav Lísa, Eva Cífková, Michal Holčapek
The hydrophilic interaction liquid chromatography (HILIC) coupled to a negative-ion electrospray ionization tandem mass spectrometry (ESI-MS/MS) method has been developed for the identification of a wide range of gangliosides in biological samples. Gangliosides consist of a backbone of sphingoid base and a polar oligosaccharide chain containing at least one sialic acid. Gangliosides are extracted by chloroform-methanol-water mixture, where an upper aqueous layer containing gangliosides and other polar lipid subclasses is further purified by C18 solid-phase extraction...
November 3, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28965321/pro-necrotic-activity-of-cationic-mastoparan-peptides-in-human-glioblastoma-multiforme-cells-via-membranolytic-action
#20
Annielle Mendes Brito da Silva, Laíz Costa Silva-Gonçalves, Fernando Augusto Oliveira, Manoel Arcisio-Miranda
Glioblastoma multiforme is the most common and lethal malignant brain tumor. Because of its complexity and heterogeneity, this tumor has become resistant to conventional therapies and the available treatment produces multiple side effects. Here, using multiple experimental approaches, we demonstrate that three mastoparan peptides-Polybia-MP1, Mastoparan X, and HR1-from solitary wasp venom exhibit potent anticancer activity toward human glioblastoma multiforme cells. Importantly, the antiglioblastoma action of mastoparan peptides occurs by membranolytic activity, leading to necrosis...
September 30, 2017: Molecular Neurobiology
keyword
keyword
74281
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"