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phosphatidylserine, brain

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https://www.readbyqxmd.com/read/28431519/semi-automatic-synthesis-and-biodistribution-of-n-2-18-f-fluoropropionyl-bis-zinc-ii-dipicolylamine-18-f-fp-dpazn2-for-ad-model-imaging
#1
Fuhua Wen, Dahong Nie, Kongzhen Hu, Ganghua Tang, Shaobo Yao, Caihua Tang
BACKGROUND: Phosphatidylserine (PS)-targeting positron emission tomography (PET) imaging with labeled small-molecule tracer is a crucial non-invasive molecule imaging method of apoptosis. In this study, semi-automatic radiosynthesis and biodistribution of N-(2-(18)F-fluoropropionyl)-bis(zinc(II)-dipicolylamine) ((18)F-FP-DPAZn2), as a potential small-molecule tracer for PET imaging of cell death in Alzheimer's disease (AD) model, were performed. METHODS: (18)F-FP-DPAZn2 was synthesized on the modified PET-MF-2V-IT-I synthesizer...
April 21, 2017: BMC Medical Imaging
https://www.readbyqxmd.com/read/28414573/phosphatidylserine-translocation-after-radiosurgery-in-an-animal-model-of-arteriovenous-malformation
#2
Newsha Raoufi Rad, Lucinda S McRobb, Zhenjun Zhao, Vivienne S Lee, Nirav J Patel, Anas Sarwar Qureshi, Michael Grace, Joshua J McHattan, Jude V Amal Raj, Hong Duong, Saleh R Kashba, Marcus A Stoodley
Phosphatidylserine (PS) is asymmetrically distributed across the plasma membrane, located predominantly on the inner leaflet in healthy cells. Translocation of PS to the outer leaflet makes it available as a target for biological therapies. We examined PS translocation after radiosurgery in an animal model of brain arteriovenous malformation (AVM). An arteriovenous fistula was created by end-to-side anastomosis of the left external jugular vein to the common carotid artery in 6-week-old, male Sprague Dawley rats...
April 17, 2017: Radiation Research
https://www.readbyqxmd.com/read/28369944/increasing-acetyl-coa-metabolism-attenuates-injury-and-alters-spinal-cord-lipid-content-in-mice-subjected-to-experimental-autoimmune-encephalomyelitis
#3
Amber C Chevalier, Thad A Rosenberger
Acetate supplementation increases brain acetyl-CoA metabolism, alters histone and non-histone protein acetylation, increases brain energy reserves, and is anti-inflammatory and neuroprotective in rat models of neuroinflammation and neuroborreliosis. To determine the impact acetate supplementation has on a mouse model of multiple sclerosis, we quantified the effect treatment had on injury progression, spinal cord lipid content, phospholipase levels, and myelin structure in mice subjected to experimental autoimmune encephalomyelitis (EAE)...
March 31, 2017: Journal of Neurochemistry
https://www.readbyqxmd.com/read/28235468/lipid-mapping-of-the-rat-brain-for-models-of-disease
#4
J Martínez-Gardeazabal, E González de San Román, M Moreno-Rodríguez, A Llorente-Ovejero, I Manuel, R Rodríguez-Puertas
Lipids not only constitute the primary component of cellular membranes and contribute to metabolism but also serve as intracellular signaling molecules and bind to specific membrane receptors to control cell proliferation, growth and convey neuroprotection. Over the last several decades, the development of new analytical techniques, such as imaging mass spectrometry (IMS), has contributed to our understanding of their involvement in physiological and pathological conditions. IMS allows researchers to obtain a wide range of information about the spatial distribution and abundance of the different lipid molecules that is crucial to understand brain functions...
February 21, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28202633/regional-changes-in-cns-and-retinal-glycerophospholipid-profiles-with-age-a-molecular-blueprint
#5
Blake R Hopiavuori, Martin-Paul Agbaga, Richard S Brush, Michael T Sullivan, William E Sonntag, Robert E Anderson
We present here a quantitative molecular blueprint of the three major glycerophospholipid (GPL) classes, phosphatidylcholine (PC), phosphatidylserine (PS), and phosphatidylethanolamine (PE), in retina and six regions of the brain in C57Bl6 mice at 2, 10, and 26 months of age. We found an age-related increase in molecular species containing saturated and monoenoic FAs and an overall decrease in the longer-chain PUFA molecular species across brain regions, with loss of DHA-containing molecular species as the most consistent and dramatic finding...
April 2017: Journal of Lipid Research
https://www.readbyqxmd.com/read/28167298/diabetes-alters-myelin-lipid-profile-in-rat-cerebral-cortex-protective-effects-of-dihydroprogesterone
#6
Gaia Cermenati, Silvia Giatti, Matteo Audano, Marzia Pesaresi, Roberto Spezzano, Donatella Caruso, Nico Mitro, Roberto Cosimo Melcangi
Due to the emerging association of diabetes with several psychiatric and neurodegenerative events, the evaluation of the effects of this pathology on the brain function has now a high priority in biomedical research. In particular, the effects of diabetes on myelin compartment have been poorly taken into consideration. To this purpose, we performed a deep lipidomic analysis of cortical myelin in the streptozotocin-induced diabetic rat model. In male rats three months of diabetes induced an extensive alterations in levels of phosphatidylcholines and phosphatidylethanolamines (the main species present in myelin membranes), plasmalogens as well as phosphatidylinositols and phosphatidylserines...
February 3, 2017: Journal of Steroid Biochemistry and Molecular Biology
https://www.readbyqxmd.com/read/28138026/preclinical-evaluation-of-sequential-combination-of-oncolytic-adenovirus-delta-24-rgd-and-phosphatidylserine-targeting-antibody-in-pancreatic-ductal-adenocarcinoma
#7
Bingbing Dai, David Roife, Ya'an Kang, Joy Gumin, Mayrim V Rios Perez, Xinqun Li, Michael Pratt, Rolf A Brekken, Juan Fueyo-Margareto, Frederick F Lang, Jason B Fleming
Delta-24-RGD (DNX-2401) is a conditional replication-competent oncolytic virus engineered to preferentially replicate in and lyse tumor cells with abnormality of p16/RB/E2F pathway. In a phase 1 clinical trial, Delta-24-RGD has shown favorable safety profile and promising clinical efficacy in brain tumor, which prompted us to evaluate its anticancer activity in pancreatic ductal adenocarcinoma (PDAC), which also has high frequency of homozygous deletion and promoter methylation of CDKN2A encoding the p16 protein...
January 30, 2017: Molecular Cancer Therapeutics
https://www.readbyqxmd.com/read/28029283/evaluation-of-procoagulant-tissue-factor-expression-in-canine-hemangiosarcoma-cell-lines
#8
Lauren E Witter, Erika J Gruber, Fabian Z X Lean, Tracy Stokol
OBJECTIVE To evaluate expression of procoagulant tissue factor (TF) by canine hemangiosarcoma cells in vitro. SAMPLES 4 canine hemangiosarcoma cell lines (SB-HSA [mouse-passaged cutaneous tumor], Emma [primary metastatic brain tumor], and Frog and Dal-1 [primary splenic tumors]) and 1 nonneoplastic canine endothelial cell line (CnAoEC). PROCEDURES TF mRNA and TF antigen expression were evaluated by quantitative real-time PCR assay and flow cytometry, respectively. Thrombin generation was measured in canine plasma and in coagulation factor-replete or specific coagulation factor-deficient human plasma by calibrated automated thrombography...
January 2017: American Journal of Veterinary Research
https://www.readbyqxmd.com/read/28028735/mapping-of-surface-exposed-epitopes-of-in-vitro-and-in-vivo-aggregated-species-of-alpha-synuclein
#9
Leire Almandoz-Gil, Veronica Lindström, Jessica Sigvardson, Philipp J Kahle, Lars Lannfelt, Martin Ingelsson, Joakim Bergström
Aggregated alpha-synuclein is the main component of Lewy bodies, intraneuronal deposits observed in Parkinson's disease and dementia with Lewy bodies. The objective of the study was to identify surface-exposed epitopes of alpha-synuclein in vitro and in vivo formed aggregates. Polyclonal immunoglobulin Y antibodies were raised against short linear peptides of the alpha-synuclein molecule. An epitope in the N-terminal region (1-10) and all C-terminal epitopes (90-140) were found to be exposed in an indirect enzyme-linked immunosorbent assay (ELISA) using recombinant monomeric, oligomeric, and fibrillar alpha-synuclein...
December 27, 2016: Cellular and Molecular Neurobiology
https://www.readbyqxmd.com/read/27913277/specific-stabilization-of-cftr-by-phosphatidylserine
#10
Ellen Hildebrandt, Netaly Khazanov, John C Kappes, Qun Dai, Hanoch Senderowitz, Ina L Urbatsch
The Cystic Fibrosis Transmembrane Conductance Regulator (CFTR, ABCC7) is a plasma membrane chloride ion channel in the ABC transporter superfamily. CFTR is a key target for cystic fibrosis drug development, and its structural elucidation would advance those efforts. However, the limited in vivo and in vitro stability of the protein, particularly its nucleotide binding domains, has made structural studies challenging. Here we demonstrate that phosphatidylserine uniquely stimulates and thermally stabilizes the ATP hydrolysis function of purified human CFTR...
February 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/27907021/the-effects-of-long-term-saturated-fat-enriched-diets-on-the-brain-lipidome
#11
Corey Giles, Ryusuke Takechi, Natalie A Mellett, Peter J Meikle, Satvinder Dhaliwal, John C Mamo
The brain is highly enriched in lipids, where they influence neurotransmission, synaptic plasticity and inflammation. Non-pathological modulation of the brain lipidome has not been previously reported and few studies have investigated the interplay between plasma lipid homeostasis relative to cerebral lipids. This study explored whether changes in plasma lipids induced by chronic consumption of a well-tolerated diet enriched in saturated fatty acids (SFA) was associated with parallel changes in cerebral lipid homeostasis...
2016: PloS One
https://www.readbyqxmd.com/read/27808224/changeover-from-signalling-to-energy-provisioning-lipids-during-transition-from-colostrum-to-mature-milk-in-the-giant-panda-ailuropoda-melanoleuca
#12
Tong Zhang, David G Watson, Rong Zhang, Rong Hou, I Kati Loeffler, Malcolm W Kennedy
Among the large placental mammals, ursids give birth to the most altricial neonates with the lowest neonatal:maternal body mass ratios. This is particularly exemplified by giant pandas. To examine whether there is compensation for the provision of developmentally important nutrients that other species groups may provide in utero, we examined changes in the lipids of colostrum and milk with time after birth in giant pandas. Lipids that are developmental signals or signal precursors, and those that are fundamental to nervous system construction, such as docosahexaenoic acid (DHA) and phosphatidylserines, appear early and then fall dramatically in concentration to a baseline at 20-30 days...
November 3, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27771897/profiling-of-signaling-proteins-in-penumbra-after-focal-photothrombotic-infarct-in-the-rat-brain-cortex
#13
Svetlana Demyanenko, Anatoly Uzdensky
In ischemic stroke, cell damage propagates from infarct core to surrounding tissue. To reveal proteins involved in neurodegeneration and neuroprotection, we explored the protein profile in penumbra surrounding the photothrombotic infarct core induced in rat cerebral cortex by local laser irradiation after Bengal Rose administration. Using antibody microarrays, we studied changes in expression of 224 signaling proteins 1, 4, or 24 h after photothrombotic infarct compared with untreated contralateral cortex...
October 22, 2016: Molecular Neurobiology
https://www.readbyqxmd.com/read/27388114/amelioration-of-scopolamine-induced-amnesia-by-phosphatidylserine-and-curcumin-in-the-day-old-chick
#14
Teresa A Barber, Edward M Edris, Paul J Levinsky, Justin M Williams, Ari R Brouwer, Shawn A Gessay
In the one-trial taste-avoidance task in day-old chicks, acetylcholine receptor activation has been shown to be important for memory formation. Injection of scopolamine produces amnesia, which appears to be very similar in type to that of Alzheimer's disease, which is correlated with low levels of acetylcholine in the brain. Traditional pharmacological treatments of Alzheimer's disease, such as cholinesterase inhibitors and glutamate receptor blockers, improve memory and delay the onset of impairments in memory compared with placebo controls...
September 2016: Behavioural Pharmacology
https://www.readbyqxmd.com/read/27231848/phosphatidylserine-targeted-liposome-for-enhanced-glioma-selective-imaging
#15
Liang Zhang, Amyn A Habib, Dawen Zhao
Phosphatidylserine (PS), which is normally intracellular, becomes exposed on the outer surface of viable endothelial cells (ECs) of tumor vasculature. Utilizing a PS-targeting antibody, we have recently established a PS-targeted liposomal (PS-L) nanoplatform that has demonstrated to be highly tumor-selective. Because of the vascular lumen-exposed PS that is immediately accessible without a need to penetrate the intact blood brain barrier (BBB), we hypothesize that the systemically administered PS-L binds specifically to tumor vascular ECs, becomes subsequently internalized into the cells and then enables its cargos to be efficiently delivered to glioma parenchyma...
June 21, 2016: Oncotarget
https://www.readbyqxmd.com/read/27220286/remodeling-of-lipid-bodies-by-docosahexaenoic-acid-in-activated-microglial-cells
#16
Marie-Eve Tremblay, Issan Zhang, Kanchan Bisht, Julie C Savage, Cynthia Lecours, Martin Parent, Vladimir Titorenko, Dusica Maysinger
BACKGROUND: Organelle remodeling processes are evolutionarily conserved and involved in cell functions during development, aging, and cell death. Some endogenous and exogenous molecules can modulate these processes. Docosahexaenoic acid (DHA), an omega-3 polyunsaturated fatty acid, has mainly been considered as a modulator of plasma membrane fluidity in brain development and aging, while DHA's role in organelle remodeling in specific neural cell types at the ultrastructural level remains largely unexplored...
2016: Journal of Neuroinflammation
https://www.readbyqxmd.com/read/27170117/megf10-is-a-receptor-for-c1q-that-mediates-clearance-of-apoptotic-cells-by-astrocytes
#17
Tal Iram, Zaida Ramirez-Ortiz, Michael H Byrne, Uwanda A Coleman, Nathan D Kingery, Terry K Means, Dan Frenkel, Joseph El Khoury
UNLABELLED: Multiple EGF-like domains 10 (Megf10) is a class F scavenger receptor (SR-F3) expressed on astrocytes and myosatellite cells, and recessive mutations in humans result in early-onset myopathy, areflexia, respiratory distress, and dysphagia (EMARDD). Here we report that Megf10-deficient mice have increased apoptotic cells in the developing cerebellum and have impaired phagocytosis of apoptotic cells by astrocytes ex vivo We also report that cells transfected with Megf10 gain the ability to phagocytose apoptotic neurons and that Megf10 binds with high affinity to C1q, an eat-me signal for apoptotic cells...
May 11, 2016: Journal of Neuroscience: the Official Journal of the Society for Neuroscience
https://www.readbyqxmd.com/read/27160923/detection-of-cancer-cells-using-sapc-dops-nanovesicles
#18
REVIEW
Harold W Davis, Nida Hussain, Xiaoyang Qi
Unlike normal cells, cancer cells express high levels of phosphatidylserine on the extracellular leaflet of their cell membrane. Exploiting this characteristic, our lab developed a therapeutic agent that consists of the fusogenic protein, saposin C (SapC) which is embedded in dioleoylphosphatidylserine (DOPS) vesicles. These nanovesicles selectively target cancer cells and induce apoptosis. Here we review the data supporting use of SapC-DOPS to locate tumors for surgical resection or for treatment. In addition, there is important evidence suggesting that SapC-DOPS may also prove to be an effective novel cancer therapeutic reagent...
2016: Molecular Cancer
https://www.readbyqxmd.com/read/27096954/optical-and-nuclear-imaging-of-glioblastoma-with-phosphatidylserine-targeted-nanovesicles
#19
Víctor M Blanco, Zhengtao Chu, Kathleen LaSance, Brian D Gray, Koon Yan Pak, Therese Rider, Kenneth D Greis, Xiaoyang Qi
Multimodal tumor imaging with targeted nanoparticles potentially offers both enhanced specificity and sensitivity, leading to more precise cancer diagnosis and monitoring. We describe the synthesis and characterization of phenol-substituted, lipophilic orange and far-red fluorescent dyes and a simple radioiodination procedure to generate a dual (optical and nuclear) imaging probe. MALDI-ToF analyses revealed high iodination efficiency of the lipophilic reporters, achieved by electrophilic aromatic substitution using the chloramide 1,3,4,6-tetrachloro-3α,6α-diphenyl glycoluril (Iodogen) as the oxidizing agent in an organic/aqueous co-solvent mixture...
May 31, 2016: Oncotarget
https://www.readbyqxmd.com/read/27018635/efferocytosis-of-apoptotic-human-papillomavirus-positive-cervical-cancer-cells-by-human-primary-fibroblasts
#20
François Hermetet, Elise Jacquin, Sophie Launay, Emilie Gaiffe, Mélanie Couturier, Fabienne Hirchaud, Patrick Sandoz, Jean-Luc Prétet, Christiane Mougin
BACKGROUND INFORMATION: Efficient clearance of apoptotic cells, named efferocytosis, is a fundamental physiological process for tissue development and homeostasis. The contribution of non-professional phagocytes like fibroblasts to efferocytosis has been established, although the underlying mechanisms are not well understood. We recently demonstrated that horizontal DNA transfer can occur through the uptake of apoptotic human papillomavirus-positive cancer cells by human primary fibroblasts leading to their transformation...
July 2016: Biology of the Cell
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