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https://www.readbyqxmd.com/read/28516761/a-facile-and-sensitive-near-infrared-fluorescence-probe-for-the-detection-of-endogenous-alkaline-phosphatase-activity-in-vivo
#1
Song-Jiao Li, Chunyan Li, Yongfei Li, Junjie Fei, Ping Wu, Bin Yang, Juan Ou-Yang, Shi-Xin Nie
Alkaline phosphatase (ALP) is an essential enzyme and widely distributes in a variety of tissues. To date, various nanomaterial and small-molecule fluorescent probes for ALP have been constructed successfully, but the emission wavelengths of these probes are in the ultraviolet or visible range, which is not beneficial for bioimaging. Herein, a merocyanine-based near-infrared (NIR) fluorescent probe named CyP is first synthesized and used to detect ALP activity. The characteristics of probe CyP are as follows: (1) The probe possesses a facile structure, which can be obtained by easy synthetic steps...
May 18, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28515299/a-diverse-range-of-novel-rna-viruses-in-geographically-distinct-honey-bee-populations
#2
Emily J Remnant, Mang Shi, Gabriele Buchmann, Tjeerd Blacquière, Edward C Holmes, Madeleine Beekman, Alyson Ashe
Understanding the diversity and consequences of viruses present in honey bees is critical to maintain pollinator health and manage the spread of disease. The viral landscape of honey bees (Apis mellifera) has changed dramatically since the emergence of the parasitic mite Varroa destructor, which increased the spread of virulent variants of viruses such as Deformed wing virus. Previous genomic studies have focused on colonies suffering from Varroa and virulent viruses, which could mask other viral species present in honey bees, resulting in a distorted view of viral diversity...
May 17, 2017: Journal of Virology
https://www.readbyqxmd.com/read/28515089/the-n-terminus-regulates-voltage-dependent-gating-of-calhm-ion-channels
#3
Jessica E Tanis, Zhongming Ma, J Kevin Foskett
CALHM1 and its C. elegans (ce) homolog, CLHM-1, belong to a new family of physiologically important ion channels that are regulated by voltage and extracellular Ca(2+) (Ca(2+)o), but lack a canonical voltage-sensing domain. Consequently, the intrinsic voltage-dependent gating mechanisms for CALHM channels are unknown. Here, we performed voltage-clamp experiments on ceCLHM-1 chimeric, deletion, insertion and point mutants to assess the role of the N-terminus (NT) in CALHM channel gating. Analyses of chimeric channels in which the ceCLHM-1 and human (h)CALHM1 N-termini were interchanged showed that the hCALHM1 NT destabilized channel-closed states, whereas the ceCLHM-1 NT had a stabilizing effect...
May 17, 2017: American Journal of Physiology. Cell Physiology
https://www.readbyqxmd.com/read/28513367/a-sense-of-belonging-unites-queen-s-nurses
#4
Crystal Oldman
In 1893, Florence Nightingale said hospitals are 'only an intermediate stage of civilization, never intended at all even to take in the whole sick population'.
May 17, 2017: Nursing Standard
https://www.readbyqxmd.com/read/28508218/recovery-of-a-paramyxovirus-the-human-metapneumovirus-from-cloned-cdna
#5
B G van den Hoogen, R A M Fouchier
Human metapneumovirus (HMPV), a single-stranded negative-sense RNA virus belonging to the family Paramyxoviridae, is associated with respiratory tract illness, primarily in young children and persons with underlying disease. Based on genetic and antigenic variation, HMPV strains are classified into two serotypes, with isolates NL/1/00 and NL/1/99 as prototypes for serotypes A and B, respectively. The development of plasmid-based reverse genetics systems for both serotypes has resulted in developments of a wide range of vaccine candidates against HMPV infection...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28507514/markovian-analysis-of-the-sequential-behavior-of-the-spontaneous-spinal-cord-dorsum-potentials-induced-by-acute-nociceptive-stimulation-in-the-anesthetized-cat
#6
Mario Martin, Javier Béjar, Gennaro Esposito, Diógenes Chávez, Enrique Contreras-Hernández, Silvio Glusman, Ulises Cortés, Pablo Rudomín
In a previous study we developed a Machine Learning procedure for the automatic identification and classification of spontaneous cord dorsum potentials (CDPs). This study further supported the proposal that in the anesthetized cat, the spontaneous CDPs recorded from different lumbar spinal segments are generated by a distributed network of dorsal horn neurons with structured (non-random) patterns of functional connectivity and that these configurations can be changed to other non-random and stable configurations after the noceptive stimulation produced by the intradermic injection of capsaicin in the anesthetized cat...
2017: Frontiers in Computational Neuroscience
https://www.readbyqxmd.com/read/28498039/seduction-and-the-self-movements-through-precarity-race-and-sexuality-in-japanese-host-clubs
#7
Pardis Mahdavi
In this article, I build on the notion of precarity to argue that Japanese host clubs are sites in which a particular kind of intimacy and sense of 'belonging' are produced. The analysis highlights host clubs as necessary spaces among many in the precarious economy and as important venues for the production of subjectivity for the male hosts (both migrant and Japanese) as well as for the migrant women who frequent these establishments. In particular, host clubs provide a lucrative employment opportunity for young men - particularly young men who are migrants from the rural parts of Japan or the Philippines (and often Japanese-Filipino young men) who are trying to make a living in the context of what has been characterised as 'precarious Japan'...
May 12, 2017: Culture, Health & Sexuality
https://www.readbyqxmd.com/read/28493780/nursing-students-experiences-of-mentorship-in-their-final-placement
#8
Robert Thomson, Angie Docherty, Raymond Duffy
AIM: The aim of this study was to explore final-year students' experiences of the mentorship role in their final placement and, in particular, to establish their expectations of the mentorship role, as well as their support and supervision needs. METHOD: Seven final-placement students were recruited from one Scottish university, using purposive sampling. Heideggerian phenomenology was chosen as the underpinning philosophical approach because of its emphasis on the 'lived experience'...
May 11, 2017: British Journal of Nursing: BJN
https://www.readbyqxmd.com/read/28493728/internal-and-community-recourses-contribution-to-level-of-posttraumatic-symptoms-the-case-of-tourists-after-the-earthquake-in-nepal-2015
#9
Haya Itzhaky, Shlomit Weiss-Dagan, Orit Taubman-Ben-Ari
OBJECTIVE: The current study focused on tourists who were caught in the middle of Nepal's, 2015 earthquake, and survived. We examined the contribution of internal (coping flexibility and emotional regulation) and community resources (sense of belonging to the community) to tourist's level of post traumatic stress disorder symptoms. METHOD: A cross-sectional sample survey was conducted to collect data from 145 tourists between 2 week to 3 months after the earthquake...
May 11, 2017: Psychological Trauma: Theory, Research, Practice and Policy
https://www.readbyqxmd.com/read/28492979/ethnic-identity-acculturation-and-12-month-psychiatric-service-utilization-among-black-and-hispanic-adults-in-the-u-s
#10
Inger Burnett-Zeigler, Yuri Lee, Kipling M Bohnert
A cross-sectional study design was used to examine the associations of ethnic identity, acculturation, and psychiatric service utilization among Wave 2 respondents of the National Epidemiologic Survey on Alcohol Related Conditions with 12-month psychiatric disorders who self-identified as Black (6587, 19%) and Hispanic (6359, 18%). Weighted multivariable regression analyses were conducted to examine the relationships between ethnic identity, acculturation, and 12-month psychiatric service utilization. Stronger ethnic identity was associated with decreased odds of using psychiatric services among Black (AOR = 0...
May 10, 2017: Journal of Behavioral Health Services & Research
https://www.readbyqxmd.com/read/28478903/experiences-of-deafblind-people-about-health-care
#11
Carmen Fernández-Valderas, Juana Macías-Seda, Eugenia Gil-García
Deafblindness is a disability resulting from the combination of visual and auditory sensory impairments, which can manifest in different levels causing special communication problems. Deafblind people have special needs that derive from difficulties in sensing, understanding, attention and a lack of the skills required to function effectively in society. Deafblindness requires specialized services, personnel specifically trained in its care and special methods for communication. OBJECTIVE: The main objective of this study is to explore the experiences of deafblind people in relation to health care throughout their lives...
May 3, 2017: Enfermería Clínica
https://www.readbyqxmd.com/read/28473787/individual-characteristics-vs-experience-an-experimental-study-on-cooperation-in-prisoner-s-dilemma
#12
Iván Barreda-Tarrazona, Ainhoa Jaramillo-Gutiérrez, Marina Pavan, Gerardo Sabater-Grande
Cooperative behavior is often assumed to depend on individuals' characteristics, such as altruism and reasoning ability. Evidence is mixed about what the precise impact of these characteristics is, as the subjects of study are generally randomly paired, generating a heterogeneous mix of the two characteristics. In this study we ex-ante create four different groups of subjects by factoring their higher or lower than the median scores in both altruism and reasoning ability. Then we use these groups in order to analyze the joint effect of the two characteristics on the individual choice of cooperating and on successful paired cooperation...
2017: Frontiers in Psychology
https://www.readbyqxmd.com/read/28468829/fluorescence-and-nmr-spectroscopy-together-with-molecular-simulations-reveal-amphiphilic-characteristics-of-a-burkholderia-biofilm-exopolysaccharide
#13
Michelle M Kuttel, Paola Cescutti, Marco Distefano, Roberto Rizzo
Biofilms are a collective mode of bacterial life in which a self-produced matrix confines cells in close proximity to each other. Biofilms confer many advantages including: protection form chemicals including antibiotics, entrapment of useful extracellular enzymes and nutrients, as well as opportunities for efficient recycling of molecules from dead cells. Biofilm matrices are aqueous gel-like structures composed of polysaccharides, proteins and DNA stabilized by intermolecular interactions which may include non-polar connections...
May 3, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28462022/exploring-the-binding-properties-and-structural-stability-of-an-opsin-in-the-chytrid-spizellomyces-punctatus-using-comparative-and-molecular-modeling
#14
Steven R Ahrendt, Edgar Mauricio Medina, Chia-En A Chang, Jason E Stajich
BACKGROUND: Opsin proteins are seven transmembrane receptor proteins which detect light. Opsins can be classified into two types and share little sequence identity: type 1, typically found in bacteria, and type 2, primarily characterized in metazoa. The type 2 opsins (Rhodopsins) are a subfamily of G-protein coupled receptors (GPCRs), a large and diverse class of seven transmembrane proteins and are generally restricted to metazoan lineages. Fungi use light receptors including opsins to sense the environment and transduce signals for developmental or metabolic changes...
2017: PeerJ
https://www.readbyqxmd.com/read/28461138/distribution-of-asic4-transcripts-in-the-adult-wild-type-mouse-brain
#15
M Hoshikawa, A Kato, H Hojo, Y Shibata, N Kumamoto, M Watanabe, S Ugawa
Acid-sensing ion channel 4 (ASIC4) belongs to the ASIC gene family of neuronal proton-gated cation channels, and is the least understood subtype among the members. Previous studies of ASIC4 expression in the mammalian central nervous system have shown that ASIC4 is abundantly expressed in the spinal cord and in various brain regions, such as the cerebral cortex, the hippocampus, and the cerebellum. However, the detailed distribution of ASIC4 transcripts in mammalian brains still remains to be elucidated. In the present study, radioactive in situ hybridization histochemistry with an ASIC4-specific cRNA probe was performed on wild-type mouse brains, followed by X-gal staining experiments with Asic4-lacZ reporter mice Asic4(tm1a(KOMP)Mbp)...
April 29, 2017: Neuroscience Letters
https://www.readbyqxmd.com/read/28456662/new-mitogenome-and-nuclear-evidence-on-the-phylogeny-and-taxonomy-of-the-highly-zoonotic-tapeworm-echinococcus-granulosus-sensu-stricto
#16
Liina Kinkar, Teivi Laurimäe, Mitra Sharbatkhori, Hossein Mirhendi, Eshrat Beigom Kia, Francisco Ponce-Gordo, Vanessa Andresiuk, Sami Simsek, Antti Lavikainen, Malik Irshadullah, Gérald Umhang, Myriam Oudni-M'rad, Gerardo Acosta-Jamett, Steffen Rehbein, Urmas Saarma
Cystic echinococcosis, a zoonotic disease caused by Echinococcus granulosus sensu lato (s. l.), is a significant global public health concern. Echinococcus granulosus s. l. is currently divided into numerous genotypes (G1-G8 and G10) of which G1-G3 are the most frequently implicated genotypes in human infections. Although it has been suggested that G1-G3 could be regarded as a distinct species E. granulosus sensu stricto (s. s.), the evidence to support this is inconclusive. Most importantly, data from nuclear DNA that provide means to investigate the exchange of genetic material between G1-G3 is lacking as none of the published nuclear DNA studies have explicitly included G2 or G3...
April 27, 2017: Infection, Genetics and Evolution
https://www.readbyqxmd.com/read/28455192/associations-between-resilience-community-belonging-and-social-participation-among-community-dwelling-older-adults-results-from-the-eastern-townships-population-health-survey
#17
Mélanie Levasseur, Mathieu Roy, Bernard Michallet, France St-Hilaire, Danielle Maltais, Mélissa Généreux
OBJECTIVE: To examine the associations between resilience, community belonging and social participation, and the moderating effect of resilience on the association between community belonging and social participation among community-dwelling older adults. DESIGN: Cross-sectional; secondary analyses of the Eastern Townships Population Health Survey. SETTING: Community. PARTICIPANTS: A sample of 2485 women and 2056 men aged 60+ was randomly selected according to area...
April 25, 2017: Archives of Physical Medicine and Rehabilitation
https://www.readbyqxmd.com/read/28451902/genetic-characterization-of-equid-herpesvirus-type-1-from-cases-of-abortion-in-poland
#18
Karol Stasiak, Magdalena Dunowska, Simon F Hills, Jerzy Rola
Equid herpesvirus type 1 (EHV-1) is a common viral infection associated with varied clinical outcomes including respiratory disease, abortion and neurological disease. We have characterized EHV-1 sequences (n = 38) obtained from cases of equine abortion in Poland between 1999 and 2016, based on sequencing of PCR products from open reading frames (ORF) 30 and 68 of the EHV-1 genome. The majority (81.6%) of sequences were not classified into any of the previously described groups based on the ORF68 sequence. The remaining sequences belonged to ORF68 group III (7...
April 27, 2017: Archives of Virology
https://www.readbyqxmd.com/read/28450857/the-functional-roles-of-the-cis-acting-elements-in-bamboo-mosaic-virus-rna-genome
#19
REVIEW
I-Hsuan Chen, Ying-Wen Huang, Ching-Hsiu Tsai
Bamboo mosaic virus (BaMV), which belongs to the genus Potexvirus in the family Alphaflexiviridae, has a single-stranded positive-sense RNA genome that is approximately 6400 nucleotides (nts) in length. Positive-sense RNA viruses can use genomic RNA as a template for translation and replication after entering a suitable host cell. Furthermore, such viral RNA is recognized by capsid protein for packaging and by viral movement protein(s) or the movement protein complex for cell-to-cell and systemic movement. Hence, viral RNA must contain signals for different functions to complete the viral infection cycle...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28445443/nutrimiraging-micromanaging-nutrient-sensing-pathways-through-nutrition-to-promote-healthy-aging
#20
REVIEW
Víctor Micó, Laura Berninches, Javier Tapia, Lidia Daimiel
Current sociodemographic predictions point to a demographic shift in developed and developing countries that will result in an unprecedented increase of the elderly population. This will be accompanied by an increase in age-related conditions that will strongly impair human health and quality of life. For this reason, aging is a major concern worldwide. Healthy aging depends on a combination of individual genetic factors and external environmental factors. Diet has been proved to be a powerful tool to modulate aging and caloric restriction has emerged as a valuable intervention in this regard...
April 26, 2017: International Journal of Molecular Sciences
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