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https://www.readbyqxmd.com/read/29339117/expression-activation-and-processing-of-a-novel-plant-milk-clotting-aspartic-protease-in-pichia-pastoris
#1
Lucía Feijoo-Siota, José Luis R Rama, Angeles Sánchez-Pérez, Tomás G Villa
Galium verum, also known as Lady's Bedstraw or Cheese Rennet, is a herbaceous perennial plant traditionally used in cheese-making. We used RACE PCR to isolate novel enzymes from Galium verum with the ability to clot milk. This approach generated two cDNA sequences (named preprogaline A and B) encoding proteins displaying the typical plant aspartic protease primary structure. Preprogaline B was expressed in the yeast Pichia pastoris, after deleting and replacing its original signal peptide with the yeast α-factor signal peptide from Saccharomyces cerevisiae...
January 12, 2018: Journal of Biotechnology
https://www.readbyqxmd.com/read/29338845/protocolized-warfarin-reversal-with-4-factor-prothrombin-complex-concentrate-versus-3-factor-prothrombin-complex-concentrate-with-recombinant-factor-viia
#2
Cassie A Barton, Marissa Hom, Nathan B Johnson, Jon Case, Ran Ran, Martin Schreiber
INTRODUCTION: Life-threatening bleeding can complicate warfarin therapy. Rapid anticoagulant reversal via replacement of vitamin-K dependent clotting factors is essential for hemostasis. We compare two methods of rapid factor replacement for warfarin reversal. METHODS: A retrospective cohort study of warfarin-treated patients experiencing life-threatening bleeding who received a reversal protocol comprised of 4F PCC or 3F PCC and rFVIIa was performed. Demographic, clinical and anticoagulant reversal information, and all adverse events attributed to warfarin reversal were recorded...
January 5, 2018: American Journal of Surgery
https://www.readbyqxmd.com/read/29336975/mechanical-and-biochemical-role-of-fibrin-within-a-venous-thrombus
#3
REVIEW
Anirudh Chandrashekar, Gurtej Singh, Jonah Garry, Nicholas Sikalas, Nicos Labropoulos
BACKGROUND: The physical properties of a venous thrombus are derived from molecular characteristics, including fibrin polymer diameter, density, branching, and cross-linking. Dense thrombi with thin, highly branched fibrin fibres and small pores in the meshwork have been observed to be more rigid, less permeable, and more resistant to lysis. The three dimensional fibrin meshwork acts as the main structure to entrap and capture erythrocytes, platelets and plasma components. Attached factors become integrated into the developing thrombus, co-localise with fibrin deposition and act in either a pro- or anticoagulant capacity...
January 12, 2018: European Journal of Vascular and Endovascular Surgery
https://www.readbyqxmd.com/read/29332812/thrombodynamics-a-new-global-coagulation-test-measurement-of-heparin-efficiency
#4
Elena I Sinauridze, Tatiana A Vuimo, Ivan D Tarandovskiy, Ruzanna A Ovsepyan, Stepan S Surov, Natalia G Korotina, Ilya I Serebriyskiy, Maxim M Lutsenko, Alexander L Sokolov, Fazoil I Ataullakhanov
The actual coagulation status may be reliably measured using only highly sensitive global functional tests; however, they are not numerous and all of them have disadvantages. Thrombodynamics (TD), a novel global coagulation test, is sensitive to hypo- and hypercoagulable states. The main properties of this test were investigated, and its capabilities for hemostasis analysis were verified through pharmacodynamic monitoring of the most widely used anticoagulants, heparins. The anticoagulant effects in the plasma of donors (n = 20) and patients after hip replacement (n = 20) spiked with unfractionated heparin or enoxaparin were measured in vitro to eliminate the influence of pharmacokinetic factors...
April 1, 2018: Talanta
https://www.readbyqxmd.com/read/29331940/screening-and-functional-exploration-of-prothrombin-arg596-related-mutations-in-chinese-venous-thromboembolism-patients
#5
Xi Wu, Lei Li, Qiulan Ding, Xuefeng Wang, Fang Wu, Wenman Wu
AIMS: Dysfunctional prothrombin residue Arg596 associated mutation has been found to precipitate venous thromboembolism (VTE). In the current study we investigated the prevalence of Arg596 associated mutations in Chinese patients with VTE and explored the functional impact of Arg596Gln mutation on coagulation function in affected patients. METHODS: Prothrombin clotting activity was measured in 267 unrelated patients with unprovoked VTE. Patients with moderately decreased activities underwent further analysis of the F2 gene...
January 13, 2018: Journal of Clinical Pathology
https://www.readbyqxmd.com/read/29330877/characterization-of-biologic-response-modifiers-in-the-supernatant-of-conventional-refrigerated-and-cryopreserved-platelets
#6
Lacey Johnson, Shereen Tan, Emily Jenkins, Ben Wood, Denese C Marks
BACKGROUND: Alternatives to room temperature storage of platelets (PLTs) are of interest to support blood banking logistics. The aim of this study was to compare the presence of biologic response modifiers (BRMs) in PLT concentrates stored under conventional room temperature conditions with refrigerated or cryopreserved PLTs. STUDY DESIGN AND METHODS: A three-arm pool-and-split study was carried out using buffy coat-derived PLTs stored in 30% plasma/70% SSP+. The three matched treatment arms were as follows: room temperature (20-24°C), cold (2-6°C), and cryopreserved (-80°C with DMSO)...
January 12, 2018: Transfusion
https://www.readbyqxmd.com/read/29330022/extracellular-matrix-contribution-to-skin-wounds-re-epithelialization
#7
REVIEW
Patricia Rousselle, Marine Montmasson, Cécile Garnier
The ability of skin to act as a barrier is primarily determined by cells that maintain the continuity and integrity of skin and restore it after injury. Cutaneous wound healing in adult mammals is a complex multi-step process that involves overlapping stages of blood clot formation, inflammation, re-epithelialization, granulation tissue formation, neovascularization, and remodeling. Under favorable conditions, epidermal regeneration begins within hours after injury and takes several days until the epithelial surface is intact due to reorganization of the basement membrane...
January 9, 2018: Matrix Biology: Journal of the International Society for Matrix Biology
https://www.readbyqxmd.com/read/29326163/lim-and-cysteine-rich-domains-1-is-required-for-thrombin-induced-smooth-muscle-cell-proliferation-and-promotes-atherogenesis
#8
Jagadeesh Janjanam, Baolin Zhang, Arul M Mani, Nikhlesh K Singh, James G Traylor, A Wayne Orr, Gadiparthi N Rao
Restenosis arises after vascular injury and is characterized by arterial wall thickening and decreased arterial lumen space. Vascular injury induces the production of thrombin, which in addition to its role in blood clotting, acts as a mitogenic and chemotactic factor. In exploring the molecular mechanisms underlying restenosis, here we identified LIM and cysteine-rich domains 1 (LMCD1) as a gene highly responsive to thrombin in human aortic smooth muscle cells (HASMCs). Of note, LMCD1 depletion inhibited proliferation of human but not murine vascular smooth muscle cells...
January 11, 2018: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/29325953/physical-risk-factors-of-hemorrhagic-complications-associated-with-angio-seal-closure-device-use-in-neurointerventional-procedures
#9
Yasuhiro Aida, Kouichi Misaki, Tomoya Kamide, Masanao Mohri, Naoyuki Uchiyama, Mitsutoshi Nakada
BACKGROUND: Few studies have demonstrated detailed physical risk factors of hemorrhagic complications (HC) associated with the Angio-Seal closure device. This retrospective study aimed to identify the risk factors of HC due to Angio-Seal use. METHODS: Data from 143 cases that underwent neurointerventional procedures that involved puncturing the femoral artery and that used an Angio-Seal to close the puncture site were reviewed. We divided these cases into HC and no HC groups and retrospectively compared age, sex, past medical and preference history, body mass index (BMI), femoral artery depth, dual antiplatelet therapy use, activated clotting time, general anesthesia use, sheath size, right femoral artery puncture, and treatment time...
January 8, 2018: World Neurosurgery
https://www.readbyqxmd.com/read/29325334/-analysis-on-the-novel-compound-heterozygous-mutation-f%C3%A2-%C2%AA-of-a-patient-with-hereditary-factor-%C3%A2-%C2%AA-deficiency
#10
K Xu, K Y Shu, F F Li, T Chen, J Liu, S S Jin, J J Guo, Z H Zhang, M H Jiang
Objective: To investigate the clinical phenotype and genotype characteristics of a Chinese hereditary factor Ⅺ deficiency pedigree. Methods: The activated partial thromboplastin time (APTT), prothrombin time (PT), FⅪ activity (FⅪ: C) were measured by clotting method using automatic coagulation analyzer. The FⅪ antigen (FⅪ: Ag) was assayed by enzyme-linked immunosorbent assay (ELISA). Fifteen exons of F11 from the proband and his pedigree members were amplified by polymerase chain reaction (PCR), then sequenced...
December 26, 2017: Zhonghua Yi Xue za Zhi [Chinese medical journal]
https://www.readbyqxmd.com/read/29324883/masp-1-of-the-complement-system-enhances-clot-formation-in-a-microvascular-whole-blood-flow-model
#11
Lorenz Jenny, József Dobó, Péter Gál, Gábor Pál, Wilbur A Lam, Verena Schroeder
The complement and coagulation systems closely interact with each other. These interactions are believed to contribute to the proinflammatory and prothrombotic environment involved in the development of thrombotic complications in many diseases. Complement MASP-1 (mannan-binding lectin-associated serine protease-1) activates coagulation factors and promotes clot formation. However, this was mainly shown in purified or plasma-based static systems. Here we describe the role of MASP-1 and complement activation in fibrin clot formation in a microvascular, whole blood flow model...
2018: PloS One
https://www.readbyqxmd.com/read/29324332/impaired-plasminogen-binding-in-patients-with-venous-thromboembolism-association-with-protein-carbonylation
#12
Jakub Siudut, Joanna Natorska, Michal Zabczyk, Dorota Zajac, Karolina Seweryn, Maria Rąpała-Kozik, Anetta Undas
INTRODUCTION: Venous thromboembolism (VTE) is associated with hypofibrinolysis. Its mechanisms are poorly understood. We investigated plasminogen-fibrin interaction and its association with fibrinolytic capacity and protein oxidation/carbonylation in VTE patients. MATERIALS AND METHODS: Plasma-purified plasminogen conversion to plasmin and surface plasmon resonance employed for plasminogen-fibrin interactions were individually evaluated in all healthy controls and non-anticoagulated patients following VTE, 10-23months since the event...
January 5, 2018: Thrombosis Research
https://www.readbyqxmd.com/read/29321328/activities-of-thrombin-and-factor-xa-are-essential-for-replication-of-hepatitis-e-virus-and-are-possibly-implicated-in-the-orf1-polyprotein-processing
#13
Gayatri D Kanade, Kunal D Pingale, Yogesh A Karpe
Hepatitis E virus (HEV) is a clinically important positive-sense RNA virus. The ORF1 of HEV encodes a non-structural polyprotein of 1693 amino acids. It is not clear whether the ORF1 polyprotein is processed into distinct enzymatic domains. Many researchers have attempted to understand the mechanisms of pORF1 processing. However, these studies gave varied results and could never convincingly establish the mechanism of pORF1 processing. In this study, we demonstrated the possible role of thrombin and factor Xa in pORF1 processing...
January 10, 2018: Journal of Virology
https://www.readbyqxmd.com/read/29318541/biotin-chasing-assay-to-evaluate-upar-stability-and-cleavage-on-the%C3%A2-surface-of-cells
#14
Vladimir Leksa, Herbert B Schiller, Hannes Stockinger
The plasminogen activation system, i.e., the fibrinolytic system, is one of the major plasma proteolytic pathways. The proteolytic conversion of the zymogen plasminogen to the active serine protease plasmin is on the cell surface catalyzed by the serine protease urokinase-type plasminogen activator (urokinase, uPA). Upon binding to the urokinase receptor (uPAR, CD87), single-chain pro-uPA is processed to double-chain uPA which in turn specifically converts cell-bound plasminogen to plasmin. Plasmin is harnessed in many physiological processes, e...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29318176/venous-thromboembolism-within-professional-american-sport-leagues
#15
Meghan Bishop, Matthew Astolfi, Eric Padegimas, Peter DeLuca, Sommer Hammoud
Background: Numerous reports have described players in professional American sports leagues who have been sidelined with a deep vein thrombosis (DVT) or a pulmonary embolism (PE), but little is known about the clinical implications of these events in professional athletes. Purpose: To conduct a retrospective review of injury reports from the National Hockey League (NHL), Major League Baseball (MLB), the National Basketball Association (NBA), and the National Football League (NFL) to take a closer look at the incidence of DVT/PE, current treatment approaches, and estimated time to return to play in professional athletes...
December 2017: Orthopaedic Journal of Sports Medicine
https://www.readbyqxmd.com/read/29317405/coagulation-and-fibrinolysis-in-hyperparathyroidism-secondary-to-vitamin-d-deficiency
#16
Laura P B Elbers, Marije Wijnberge, Joost C M Meijers, Dennis C W Poland, Dees P M Brandjes, Eric Fliers, Victor E A Gerdes
INTRODUCTION: Abnormal coagulation tests have been observed in patients with primary hyperparathyroidism (HPT) suggesting a prothrombotic effect of parathyroid hormone (PTH). Vitamin D deficiency (VIDD) is the most frequent cause of secondary HPT. Aim of our study was to investigate the influence of HPT secondary to moderate-severe VIDD and vitamin D replacement on the coagulation and fibrinolysis system. SUBJECTS AND METHODS: Prospective cohort study of patients with vitamin D<25 nmol/l with and without HPT, and a control group of patients on vitamin D suppletion...
January 9, 2018: Endocrine Connections
https://www.readbyqxmd.com/read/29314633/leucocyte-and-platelet-rich-fibrin-a-carrier-of-autologous-multipotent-cells-for-regenerative-medicine
#17
Rosa Di Liddo, Thomas Bertalot, Alessio Borean, Ivan Pirola, Alberto Argentoni, Sandra Schrenk, Carola Cenzi, Stefano Capelli, Maria Teresa Conconi, Pier Paolo Parnigotto
The wound healing is a complex process wherein inflammation, proliferation and regeneration evolve according to a spatio-temporal pattern from the activation of coagulation cascade to the formation of a plug clot including fibrin matrix, blood-borne cells and cytokines/growth factors. Creating environments conducive to tissue repair, the haemoderivatives are commonly proposed for the treatment of hard-to-heal wounds. Here, we explored in vitro the intrinsic regenerative potentialities of a leucocyte- and platelet-rich fibrin product, known as CPL-MB, defining the stemness grade of cells sprouting from the haemoderivative...
January 5, 2018: Journal of Cellular and Molecular Medicine
https://www.readbyqxmd.com/read/29314552/australian-multicentre-study-of-current-real-world-prophylaxis-practice-in-severe-and-moderate-haemophilia-a-and-b
#18
J A Mason, S Parikh, H Tran, J Rowell, S McRae
INTRODUCTION: With the emergence of novel treatment products for haemophilia and an increasing focus on the benefits of pharmacokinetic driven individualized prophylaxis, robust national data with regard to current patterns of factor consumption and adherence are required. AIM: To characterize current Australian practice with regard to use of prophylactic clotting factor infusions in patients with moderate or severe haemophilia A (HA) and haemophilia B (HB). METHODS: This was a retrospective, non-interventional study utilizing Australian Bleeding Disorder Registry (ABDR) data collected over a 12 month period...
January 3, 2018: Haemophilia: the Official Journal of the World Federation of Hemophilia
https://www.readbyqxmd.com/read/29312706/hemorrhage-under-veno-venous-extracorporeal-membrane-oxygenation-in-acute-respiratory-distress-syndrome-patients-a-retrospective-data-analysis
#19
Stefan Kreyer, Thomas Muders, Nils Theuerkauf, Juliane Spitzhüttl, Torsten Schellhaas, Jens-Christian Schewe, Ulf Guenther, Hermann Wrigge, Christian Putensen
Background: Despite being still invasive and challenging, technical improvement has resulted in broader and more frequent application of extracorporeal membrane oxygenation (ECMO), to prevent hypoxemia and to reduce invasiveness of mechanical ventilation (MV). Heparin-coated ECMO-circuits are currently standard of care, in addition to heparin based anticoagulation (AC) regimen guided by activated clotting time (ACT) or activated partial thromboplastin time (aPTT). Despite these advances, a reliable prediction of hemorrhage is difficult and the risk of hemorrhagic complication remains unfortunately high...
December 2017: Journal of Thoracic Disease
https://www.readbyqxmd.com/read/29309730/the-exploration-on-blood-coagulation-of-n-alkyl-chitosan-nanofiber-membrane-in-vitro
#20
Xiaoyan Wang, Guan Jing, Xupin Zhuang, Zhihong Li, Shujie Huang, Jian Yang, Changjun Liu, Fan Li, Feng Tian, Jimin Wu, Zhan Shu
N-alkylated chitosan (NACS) may improve blood clotting efficiency of chitosan (CS). To study its blood coagulation capability, a series of NACS with various carbon chain lengths and degrees of substitution (DS) of alkyl groups were synthesized and characterized by FTIR, NMR, elemental analysis, and X-Ray diffraction (XRD). The corresponding NACS nanofiber membranes (NACS-NM) were subsequently fabricated by electronic spinning technique. SEM, XRD, DSC, surface area, porosity, contact angle, blood absorption and mechanical property were used to characterize the basic property of CS-NM/NACS-NM...
January 8, 2018: Biomacromolecules
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