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https://www.readbyqxmd.com/read/28898409/combined-effect-of-ultrasound-and-mild-temperatures-on-the-inactivation-of-e-coli-in-fresh-carrot-juice-and-changes-on-its-physicochemical-characteristics
#1
Prashant Raj Pokhrel, Daniela Bermúdez-Aguirre, Héctor E Martínez-Flores, M Guadalupe Garnica-Romo, Shyam Sablani, Juming Tang, Gustavo V Barbosa-Cánovas
The combination of ultrasound and mild temperatures to process fruits and vegetables juices is a novel approach that is showing promising results for microbial inactivation and preservation of bioactive compounds and sensory attributes. This study centers on investigating the inactivation of Escherichia coli (ATCC 11755) in carrot juice as a result of the combined effect of ultrasound (24 kHz frequency, 120 μm, and 400 W) with temperature (50, 54, and 58 °C) and processing time (0 to 10 min). In addition, the possible changes in physicochemical properties and the retention of bioactive compounds after processing were analyzed...
September 12, 2017: Journal of Food Science
https://www.readbyqxmd.com/read/28867511/glutamine-and-citrulline-concentrations-reflect-nitric-oxide-synthesis-in-the-human-nervous-system
#2
I Pérez-Neri, J Ramírez-Bermúdez, C Ojeda-López, S Montes, J L Soto-Hernández, C Ríos
INTRODUCTION: Although citrulline is produced by nitric oxide (NO) synthase upon activation of the NMDA glutamate receptor, nitrite and nitrate (NOx) concentration is considered the best marker of NO synthesis, as citrulline is also metabolised by other enzymes. This study analyses the correlation between human cerebrospinal fluid NOx and citrulline concentrations in order to determine the extent to which citrulline reflects NO synthesis and glutamatergic neurotransmission. METHODS: Participants were patients with acute neurological diseases undergoing lumbar puncture (n=240)...
August 31, 2017: Neurología: Publicación Oficial de la Sociedad Española de Neurología
https://www.readbyqxmd.com/read/28809789/comparison-of-the-effects-of-goat-dairy-and-cow-dairy-based-breakfasts-on-satiety-appetite-hormones-and-metabolic-profile
#3
Elehazara Rubio-Martín, Eva García-Escobar, Maria-Soledad Ruiz de Adana, Fuensanta Lima-Rubio, Laura Peláez, Angel-María Caracuel, Francisco-Javier Bermúdez-Silva, Federico Soriguer, Gemma Rojo-Martínez, Gabriel Olveira
The satiating effects of cow dairy have been thoroughly investigated; however, the effects of goat dairy on appetite have not been reported so far. Our study investigates the satiating effect of two breakfasts based on goat or cow dairy and their association with appetite related hormones and metabolic profile. Healthy adults consumed two breakfasts based on goat (G-Breakfast) or cow (C-Breakfast) dairy products. Blood samples were taken and VAS tests were performed at different time points. Blood metabolites were measured and Combined Satiety Index (CSI) and areas under the curves (AUC) were calculated...
August 15, 2017: Nutrients
https://www.readbyqxmd.com/read/28802861/contrasting-effects-of-vortioxetine-and-paroxetine-on-pineal-gland-biochemistry-in-a-tryptophan-depletion-model-of-depression-in-female-rats
#4
M Franklin, N Hlavacova, Y Li, I Bermudez, A Csanova, C Sanchez, D Jezova
We studied the effects of the multi-modal antidepressant, vortioxetine and the SSRI, paroxetine on pineal melatonin and monoamine synthesis in a sub-chronic tryptophan (TRP) depletion model of depression based on a low TRP diet. Female Sprague-Dawley rats were randomised to groups a) control, b) low TRP diet, c) low TRP diet+paroxetine and d) low TRP diet+vortioxetine. Vortioxetine was administered via the diet (0.76mg/kg of food weight) and paroxetine via drinking water (10mg/kg/day) for 14days. Both drugs resulted in SERT occupancies >90%...
August 10, 2017: Progress in Neuro-psychopharmacology & Biological Psychiatry
https://www.readbyqxmd.com/read/28763050/3d-hydrogel-based-polymeric-nanoreactors-for-silver-nano-antimicrobial-composites-generation
#5
Albanelly Soto-Quintero, Ángel Romo-Uribe, Víctor H Bermúdez-Morales, Isabel Quijada-Garrido, Nekane Guarrotxena
This study underscores the development of Ag hydrogel nanocomposites, as smart substrates for antibacterial uses, via innovative in situ reactive and reduction pathways. To this end, two different synthetic strategies were used. Firstly thiol-acrylate (PSA) based hydrogels were attained via thiol-ene and radical polymerization of polyethylene glycol (PEG) and polycaprolactone (PCL). As a second approach, polyurethane (PU) based hydrogels were achieved by condensation polymerization from diisocyanates and PCL and PEG diols...
August 1, 2017: Nanomaterials
https://www.readbyqxmd.com/read/28740975/tyrosol-and-its-metabolites-as-antioxidative-and-anti-inflammatory-molecules-in-human-endothelial-cells
#6
Francisco J G Muriana, Sergio Montserrat-de la Paz, Ricardo Lucas, Beatriz Bermudez, Sara Jaramillo, Juan C Morales, Rocio Abia, Sergio Lopez
Tyrosol (Tyr) is a phenolic compound found in virgin olive oil. After ingestion, Tyr undergoes extensive first pass intestinal/hepatic metabolism. However, knowledge about the biological effects of Tyr metabolites is scarce. We chemically synthesized Tyr glucuronate (Tyr-GLU) and sulphate (Tyr-SUL) metabolites and explored their properties against oxidative stress and inflammation in TNF-α-treated human umbilical vein endothelial cells (hECs). Tyr and Tyr-SUL prevented the rise of reactive oxygen species, the depletion of glutathione, and the down-regulation of glutathione peroxidase 1, glutamate-cysteine ligase catalytic subunit, and heme oxygenase-1 genes...
August 1, 2017: Food & Function
https://www.readbyqxmd.com/read/28703221/manipulation-of-nuclear-architecture-through-crispr-mediated-chromosomal-looping
#7
Stefanie L Morgan, Natasha C Mariano, Abel Bermudez, Nicole L Arruda, Fangting Wu, Yunhai Luo, Gautam Shankar, Lin Jia, Huiling Chen, Ji-Fan Hu, Andrew R Hoffman, Chiao-Chain Huang, Sharon J Pitteri, Kevin C Wang
Chromatin looping is key to gene regulation, yet no broadly applicable methods to selectively modify chromatin loops have been described. We have engineered a method for chromatin loop reorganization using CRISPR-dCas9 (CLOuD9) to selectively and reversibly establish chromatin loops. We demonstrate the power of this technology to selectively modulate gene expression at targeted loci.
July 13, 2017: Nature Communications
https://www.readbyqxmd.com/read/28667436/comparison-of-quality-of-life-among-community-dwelling-older-adults-with-the-frailty-phenotype
#8
Sergio Sánchez-García, Katia Gallegos-Carrillo, María Claudia Espinel-Bermudez, Svetlana V Doubova, Rosalinda Sánchez-Arenas, Carmen García-Peña, Antoni Salvà, Silvia C Briseño-Fabian
PURPOSE: To compare the perception of the quality of life (QOL) of community-dwelling older adults with the phenotype of frailty. METHODS: Cross-sectional analysis of baseline data of the "Cohort of Obesity, Sarcopenia and Frailty of Mexican Older Adults" (COSFOMA). Operationalization of frailty was carried out using the phenotype as follows: weight loss, self-report of exhaustion, low physical activity, slow gait, and weakness. QOL was measured using two scales: World Health Organization Quality of Life of Older Adults (WHOQOL-OLD), which is a specific instrument for the elderly population, and Short Form-36 Health Survey (SF-36), a generic instrument to evaluate the QOL related to health...
June 30, 2017: Quality of Life Research
https://www.readbyqxmd.com/read/28655286/a-group-ii-metabotropic-glutamate-receptor-3-mglu3-grm3-isoform-implicated-in-schizophrenia-interacts-with-canonical-mglu3-and-reduces-ligand-binding
#9
Aintzane García-Bea, Isabel Bermudez, Paul J Harrison, Tracy A Lane
As well as being expressed as a full-length transcript, the group II metabotropic glutamate receptor 3 (GRM3, mGlu3) gene is expressed as an mRNA isoform which lacks exon 4 (GRM3Δ4) and which is predicted to encode a protein with a novel C terminus (called mGlu3Δ4). This variant may contribute to the mechanism by which GRM3 acts as a schizophrenia risk gene. However, little is known about the properties or function of mGlu3Δ4. Here, using transiently transfected HEK293T/17 cells, we confirm that GRM3Δ4 cDNA is translated, with mGlu3Δ4 existing as a homodimer as well as a monomer, and localizing primarily to cell membranes including the plasma membrane...
June 1, 2017: Journal of Psychopharmacology
https://www.readbyqxmd.com/read/28644236/cyp1b1-cytopathy-uncommon-phenotype-of-a-homozygous-cyp1b1-deletion-as-internal-corneal-ulcer-of-von-hippel
#10
Valeria Oliva-Biénzobas, Alejandro Navas, Mirena C Astiazarán, Oscar Francisco Chacón-Camacho, Jose A Bermúdez-Magner, Mariana Takane, Enrique Graue-Hernández, Juan Carlos Zenteno
PURPOSE: To report a mutation of CYP1B1 in a newborn with a rare phenotype without the classic features of anterior segment dysgenesis or congenital glaucoma. METHODS: The newborn presented with diffuse corneal edema and bilaterally elevated intraocular pressure (IOP). Ophthalmological examination, ultrasound, and ultrasound biomicroscopy were performed; congenital infections were ruled out. Genetic analysis was performed. The patient underwent penetrating keratoplasty and goniotomy in a single surgical time...
October 2017: Cornea
https://www.readbyqxmd.com/read/28636003/in-vitro-modulation-of-gut-microbiota-by-whey-protein-to-preserve-intestinal-health
#11
T Sánchez-Moya, R López-Nicolás, D Planes, C A González-Bermúdez, G Ros-Berruezo, C Frontela-Saseta
The effect of several types of whey milk - cow, sheep, goat and a mixture of them (60 : 20 : 20, respectively) - was assessed in the human gut microbiota. The prebiotic potential of these substrates was evaluated through in vitro gastrointestinal digestion following faecal batch culture fermentations (mimicking colonic fermentation) for 48 hours, using faeces from normal-weight (NW) and obese (OB) donors. Throughout the fermentation process, pH, gas production, short chain and branched fatty acids (SCFA-BCFA) were measured, as well as the changes of microbiota using qPCR...
June 21, 2017: Food & Function
https://www.readbyqxmd.com/read/28629710/anaerobic-digestion-of-tuna-waste-for-the-production-of-volatile-fatty-acids
#12
Noela Bermúdez-Penabad, Christian Kennes, Maria C Veiga
Fish canning industries generate a significant amount of solid waste that can be digested anaerobically into volatile fatty acids (VFA). The aim of this research was to study the effect of various pHs, ranging from 5.0 to 10.0, and percentage of total solids on the anaerobic digestion of tuna waste into VFA, both in batch assays and continuous reactor. The production of VFA was affected by pH and was significantly higher under alkaline conditions. At pH 8.0, the VFA production reached 30,611mgCOD/L. The VFA mainly consisted of acetic, propionic, n-butyric and i-valeric acids...
June 16, 2017: Waste Management
https://www.readbyqxmd.com/read/28604646/effect-of-supercritical-carbon-dioxide-extraction-parameters-on-the-biological-activities-and-metabolites-present-in-extracts-from-arthrospira-platensis
#13
Diego A Esquivel-Hernández, José Rodríguez-Rodríguez, Sara P Cuéllar-Bermúdez, J Saúl García-Pérez, Elena I Mancera-Andrade, Jade E Núñez-Echevarría, Aura Ontiveros-Valencia, Magdalena Rostro-Alanis, Rebeca M García-García, J Antonio Torres, Wei Ning Chen, Roberto Parra-Saldívar
Arthrospira platensis was used to obtain functional extracts through supercritical carbon dioxide extraction (SFE-CO₂). Pressure (P), temperature (T), co-solvent (CX), static extraction (SX), dispersant (Di) and dynamic extraction (DX) were evaluated as process parameters through a Plackett-Burman design. The maximum extract yield obtained was 7.48 ± 0.15% w/w. The maximum contents of bioactive metabolites in extracts were 0.69 ± 0.09 µg/g of riboflavin, 5.49 ± 0.10 µg/g of α-tocopherol, 524.46 ± 0...
June 12, 2017: Marine Drugs
https://www.readbyqxmd.com/read/28556669/impact-on-area-level-physical-activity-following-the-implementation-of-fitness-zones-in-montevideo-uruguay
#14
Cecilia DelCampo, Veronica Tutte, Gustavo Bermudez, Diana C Parra
BACKGROUND: To measure the level of physical activity (PA) of the users of an urban park before and after the installation of two Fitness Zones (FZ) and to assess the impact of that intervention on the users' level of PA. METHODS: The SOPARC method was applied in the urban plaza Liber Seregni in Montevideo, fourteen different areas were mapped and then re-categorized as fitness (for physical activities, including sports), green and paved zones. Observations were made in the spring (September-October) of 2011 and 2014, before and after placing two FZ...
May 30, 2017: Journal of Physical Activity & Health
https://www.readbyqxmd.com/read/28480508/cultivation-of-solanum-tuberosum-in-a-former-mining-district-for-a-safe-human-consumption-integrating-simulated-digestion
#15
M J Sierra, R López-Nicolás, C A González-Bermúdez, C Frontela-Saseta, R Millán
BACKGROUND: Potato (Solanum tuberosum) is a global crop and by far the most important non-cereal crop in the world. Therefore, it is necessary to assure its safe consumption. This is especially relevant in the case of its cultivation in abandoned mining areas, where the population tends to return to agriculture. In the present work, the objective is to evaluate the contribution to the diet of nutrients and contaminants of potato grown in soils from the Almadén area (mining district) by studying the intestinal absorption (in vitro) of the tuber taking into account the preparation methods for its consumption...
May 8, 2017: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/28477318/molecular-characterization-and-clinical-interpretation-of-brca1-brca2-variants-in-families-from-murcia-south-eastern-spain-with-hereditary-breast-and-ovarian-cancer-clinical-pathological-features-in-brca-carriers-and-non-carriers
#16
Xavier Gabaldó Barrios, Mª Desamparados Sarabia Meseguer, Miguel Marín Vera, Ana Isabel Sánchez Bermúdez, José Antonio Macías Cerrolaza, Pilar Sánchez Henarejos, Marta Zafra Poves, Mª Rosario García Hernández, Encarna Cuevas Tortosa, Ángeles Aliaga Baño, Verónica Castillo Guardiola, Pedro Martínez Hernández, Isabel Tovar Zapata, Enrique Martínez Barba, Francisco Ayala de la Peña, José Luis Alonso Romero, José Antonio Noguera Velasco, Francisco Ruiz Espejo
This is the first study performed in Murcia (south-eastern Spain) in which 592 families with hereditary breast and ovarian cancer were identified thanks to Genetic Counselling Units from this area over 6 years. Diagnostic performance was 18.1% and 194 different genetic variants were obtained. Variants with uncertain significance accounted for only 5.6% of the total number of reports, so our population has been well characterised. In BRCA1 gene, two novel variants were found (c.1859delT and c.3205C > T) and the most frequently detected mutations were c...
May 5, 2017: Familial Cancer
https://www.readbyqxmd.com/read/28453060/-physician-patient-relationship-impact-on-promotion-and-prevention-campaigns-for-patients-with-hiv-in-medellin
#17
Viviana Bermúdez-Román, Lemy Bran-Piedrahita, Lucía Palacios-Moya, Isabel C Posada-Zapata
Objective To understand the impact that healthcare professionals have on the perception of health promotion and disease prevention campaigns of individuals with HIV in Medellin, Colombia in 2012. MATERIALS AND METHODS: The research was conducted using a qualitative approach, based on the Strauss and Corbin´s Grounded Theory; thirteen people were interviewed during two sequential steps. The interviews were transcribed and analyzed in accordance with the theory mentioned above, by performing an open and axial coding on the data found...
August 2016: Revista de Salud Pública
https://www.readbyqxmd.com/read/28414293/inherited-gins1-deficiency-underlies-growth-retardation-along-with-neutropenia-and-nk-cell-deficiency
#18
Julien Cottineau, Molly C Kottemann, Francis P Lach, Young-Hoon Kang, Frédéric Vély, Elissa K Deenick, Tomi Lazarov, Laure Gineau, Yi Wang, Andrea Farina, Marie Chansel, Lazaro Lorenzo, Christelle Piperoglou, Cindy S Ma, Patrick Nitschke, Aziz Belkadi, Yuval Itan, Bertrand Boisson, Fabienne Jabot-Hanin, Capucine Picard, Jacinta Bustamante, Céline Eidenschenk, Soraya Boucherit, Nathalie Aladjidi, Didier Lacombe, Pascal Barat, Waseem Qasim, Jane A Hurst, Andrew J Pollard, Holm H Uhlig, Claire Fieschi, Jean Michon, Vladimir P Bermudez, Laurent Abel, Jean-Pierre de Villartay, Frédéric Geissmann, Stuart G Tangye, Jerard Hurwitz, Eric Vivier, Jean-Laurent Casanova, Agata Smogorzewska, Emmanuelle Jouanguy
Inborn errors of DNA repair or replication underlie a variety of clinical phenotypes. We studied 5 patients from 4 kindreds, all of whom displayed intrauterine growth retardation, chronic neutropenia, and NK cell deficiency. Four of the 5 patients also had postnatal growth retardation. The association of neutropenia and NK cell deficiency, which is unusual among primary immunodeficiencies and bone marrow failures, was due to a blockade in the bone marrow and was mildly symptomatic. We discovered compound heterozygous rare mutations in Go-Ichi-Ni-San (GINS) complex subunit 1 (GINS1, also known as PSF1) in the 5 patients...
May 1, 2017: Journal of Clinical Investigation
https://www.readbyqxmd.com/read/28408371/leukocyte-overexpression-of-intracellular-nampt-attenuates-atherosclerosis-by-regulating-ppar%C3%AE-dependent-monocyte-differentiation-and-function
#19
Beatriz Bermudez, Tuva Borresdatter Dahl, Indira Medina, Mathijs Groeneweg, Sverre Holm, Sergio Montserrat-de la Paz, Mat Rousch, Jeroen Otten, Veronica Herias, Lourdes M Varela, Trine Ranheim, Arne Yndestad, Almudena Ortega-Gomez, Rocio Abia, Laszlo Nagy, Pal Aukrust, Francisco J G Muriana, Bente Halvorsen, Erik Anna Leonardus Biessen
OBJECTIVE: Extracellular nicotinamide phosphoribosyltransferase (eNAMPT) mediates inflammatory and potentially proatherogenic effects, whereas the role of intracellular NAMPT (iNAMPT), the rate limiting enzyme in the salvage pathway of nicotinamide adenine dinucleotide (NAD)(+) generation, in atherogenesis is largely unknown. Here we investigated the effects of iNAMPT overexpression in leukocytes on inflammation and atherosclerosis. APPROACH AND RESULTS: Low-density lipoprotein receptor-deficient mice with hematopoietic overexpression of human iNAMPT (iNAMPT(hi)), on a western type diet, showed attenuated plaque burden with features of lesion stabilization...
June 2017: Arteriosclerosis, Thrombosis, and Vascular Biology
https://www.readbyqxmd.com/read/28375611/phase-transition-dielectric-properties-and-ionic-transport-in-the-ch3-2nh2-pbi3-organic-inorganic-hybrid-with-2h-hexagonal-perovskite-structure
#20
A García-Fernández, J M Bermúdez-García, S Castro-García, A L Llamas-Saiz, R Artiaga, J López-Beceiro, S Hu, W Ren, A Stroppa, M Sánchez-Andújar, M A Señarís-Rodríguez
In this work, we focus on [(CH3)2NH2]PbI3, a member of the [AmineH]PbI3 series of hybrid organic-inorganic compounds, reporting a very easy mechanosynthesis route for its preparation at room temperature. We report that this [(CH3)2NH2]PbI3 compound with 2H-perovskite structure experiences a first-order transition at ≈250 K from hexagonal symmetry P63/mmc (HT phase) to monoclinic symmetry P21/c (LT phase), which involves two cooperative processes: an off-center shift of the Pb(2+) cations and an order-disorder process of the N atoms of the DMA cations...
April 4, 2017: Inorganic Chemistry
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