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https://www.readbyqxmd.com/read/28009074/new-monoclonal-antibodies-to-defined-cell-surface-proteins-on-human-pluripotent-stem-cells
#1
Carmel M O'Brien, Hun S Chy, Qi Zhou, Shiri Blumenfeld, Jack W Lambshead, Xiaodong Liu, Joshua Kie, Bianca D Capaldo, Tung-Liang Chung, Timothy E Adams, Tram Phan, John D Bentley, William J Mckinstry, Karen Oliva, Paul J Mcmurrick, Yu-Chieh Wang, Fernando J Rossello, Geoffrey J Lindeman, Di Chen, Thierry Jarde, Amander T Clark, Helen E Abud, Jane E Visvader, Christian M Nefzger, Jose M Polo, Jeanne F Loring, Andrew L Laslett
The study and application of human pluripotent stem cells (hPSCs) will be enhanced by the availability of well-characterised monoclonal antibodies (mAbs) detecting cell-surface epitopes. Here we report generation of seven new mAbs that detect cell surface proteins present on live and fixed human ES cells (hESCs) and human iPS cells (hiPSCs), confirming our previous prediction that these proteins were present on the cell surface of hPSCs. The mAbs all show a high correlation with POU5F1 (OCT4) expression and other hPSC surface markers (TRA-160 and SSEA-4) in hPSC cultures and detect rare OCT4 positive cells in differentiated cell cultures...
December 23, 2016: Stem Cells
https://www.readbyqxmd.com/read/27998922/measuring-time-spent-caring-for-drinkers-and%C3%A2-their-dependents
#2
Heng Jiang, Sarah Callinan, Anne-Marie Laslett, Robin Room
AIMS: To quantify the extent of time spent by family and friends caring for drinkers and their dependents, to estimate the cost of this time and to measure which factors predict time spent caring. METHODS: Data are from a nationwide Alcohol's Harm to Others Survey of 2649 Australians, in which 778 respondents reported they were harmed by a known drinker. Time spent on four caring activities was self-reported by these respondents and tallied to estimate how many hours they spent caring for the drinker, the drinker's children or other dependents...
January 2017: Alcohol and Alcoholism: International Journal of the Medical Council on Alcoholism
https://www.readbyqxmd.com/read/27936246/diagnostic-accuracy-of-clinical-examination-and-imaging-findings-for-identifying-subacromial-pain
#3
Angela Cadogan, Peter J McNair, Mark Laslett, Wayne A Hing
BACKGROUND: The diagnosis of subacromial pathology is limited by the poor accuracy of clinical tests for specific pathologies. The aim of this study was to estimate the diagnostic accuracy of clinical examination and imaging features for identifying subacromial pain (SAP) defined by a positive response to diagnostic injection, and to evaluate the influence of imaging findings on the clinical diagnosis of SAP. METHODS AND FINDINGS: In a prospective, diagnostic accuracy design, 208 consecutive patients presenting to their primary healthcare practitioner for the first time with a new episode of shoulder pain were recruited...
2016: PloS One
https://www.readbyqxmd.com/read/27836676/cross-sectional-and-longitudinal-associations-between-serum-inflammatory-cytokines-and-knee-bone-marrow-lesions-in-patients-with-knee-osteoarthritis
#4
Z Zhu, P Otahal, B Wang, X Jin, L L Laslett, A Wluka, B Antony, W Han, X Wang, T Winzenberg, F Cicuttini, G Jones, C Ding
OBJECTIVE: To describe cross-sectional and longitudinal associations between serum levels of interleukin (IL) - 6, IL-17A, IL-17F, IL-23 and knee bone marrow lesions (BMLs) in patients with knee osteoarthritis (OA). DESIGN: Patients (n = 192) with symptomatic knee OA (mean 63 years, range 50-79, female 53%) were assessed at baseline and after 24 months. At each time point, serum IL-6, IL-17A, IL-17F and IL-23 were measured using Bio-Plex(®) Multiplex Immunoassays with Luminex xMAP technology...
November 9, 2016: Osteoarthritis and Cartilage
https://www.readbyqxmd.com/read/27805281/moderate-to-vigorous-physical-activity-but-not-sedentary-time-is-associated-with-musculoskeletal-health-outcomes-in-a-cohort-of-australian-middle-aged-women
#5
Feitong Wu, Karen Wills, Laura L Laslett, Brian Oldenburg, Graeme Jones, Tania Winzenberg
Associations between physical activity and time spent sedentary and musculoskeletal outcomes remain unclear in middle-aged adults. This study aimed to describe associations between objectively-measured physical activity and sedentary time and musculoskeletal health outcomes in middle-aged women. This cross-sectional study from a population-based sample of 309 women (age 36 to 57 years) examined associations of total physical activity (accelerometer counts/min of wear time), and time spent sedentary, in light physical activities and moderate-to-vigorous physical activities (MVPA) (by Actigraph GT1M accelerometer) with lumbar spine (LS) and femoral neck (FN) bone mineral density (BMD) (by dual-energy X-ray absorptiometry), lower limb muscle strength (LMS), and functional mobility and balance tests (timed up and go test [TUG], functional reach test [FRT], lateral reach test [LRT], and step test [ST]) using linear regression...
November 2, 2016: Journal of Bone and Mineral Research: the Official Journal of the American Society for Bone and Mineral Research
https://www.readbyqxmd.com/read/27803343/knee-pain-predicts-subsequent-shoulder-pain-and-the-association-is-mediated-by-leg-weakness-longitudinal-observational-data-from-the-osteoarthritis-initiative
#6
Laura L Laslett, Petr Otahal, Elizabeth M A Hensor, Sarah R Kingsbury, Philip G Conaghan
OBJECTIVE: To assess whether the "spread" of joint pain is related to pain-associated muscle loss in 1 joint leading to increased loading and subsequent pain in other joints. METHODS: Associations between persistent knee pain (pain in 1 or 2 knees over 0-3 years vs no persistent pain) and incident shoulder pain at Year 4 were examined in participants from the longitudinal National Institutes of Health Osteoarthritis Initiative. Associations were assessed using log multinomial modeling, adjusted for age, sex, body mass index, depression score, other lower limb pain, and baseline leg weakness (difficulty standing from a sitting position)...
November 2016: Journal of Rheumatology
https://www.readbyqxmd.com/read/27631092/cut-points-for-associations-between-vitamin-d-status-and-multiple-musculoskeletal-outcomes-in-middle-aged-women
#7
F Wu, K Wills, L L Laslett, B Oldenburg, M J Seibel, G Jones, T Winzenberg
: This was the first study examining optimal vitamin D status for musculoskeletal health in middle-aged women. A 25-hydroxyvitamin D level of at least 29 to 33 nmol/L appears required for optimal musculoskeletal health, but the current cut-off of 50 nmol/L may be warranted. INTRODUCTION: This study aimed to determine whether cut-points exist for associations between serum 25-hydroxyvitamin D (25OHD) and musculoskeletal health outcomes in middle-aged women, below which greater 25OHD levels are associated with musculoskeletal health benefits and above which no such associations exist...
September 9, 2016: Osteoporosis International
https://www.readbyqxmd.com/read/27390184/pain-at-sites-outside-the-knee-predicts-knee-cartilage-volume-loss-in-elderly-people-without-knee-osteoarthritis-a-prospective-study
#8
Feng Pan, Laura Laslett, Jing Tian, Flavia Cicuttini, Tania Winzenberg, Changhai Ding, Graeme Jones
OBJECTIVE: Pain is common in the elderly. Knee pain may predict knee cartilage loss, but whether generalised pain is associated with knee cartilage loss is unclear. This study, therefore, aimed to determine whether pain at multiple sites predicts knee cartilage volume loss amongst community-dwelling older adults, and if so, to explore potential mechanisms. METHODS: Data from the prospective Tasmanian Older Adult Cohort study was utilised (n=394; mean age, 63 years; range 52 to 79)...
July 7, 2016: Arthritis Care & Research
https://www.readbyqxmd.com/read/27390162/associations-between-fat-mass-and-multi-site-pain-a-5-year-longitudinal-study
#9
Feng Pan, Laura Laslett, Leigh Blizzard, Flavia Cicuttini, Tania Winzenberg, Changhai Ding, Graeme Jones
OBJECTIVE: Pain is common in older adults and typically involves multiple sites. Obesity is an important risk factor in the pathogenesis of pain and multi-site pain (MSP). This study aimed to examine longitudinal associations between fat mass and MSP, and to explore the potential mechanisms of any associations. METHODS: Data from a longitudinal population-based study of older adults (n=1099) was utilized with measurements at baseline and after 2.6 and 5.1 years...
July 7, 2016: Arthritis Care & Research
https://www.readbyqxmd.com/read/26946066/patellofemoral-bone-marrow-lesions-natural-history-and-associations-with-pain-and-structure
#10
Zhaohua Zhu, Changhai Ding, Xingzhong Jin, Benny Antony, Weiyu Han, Laura L Laslett, Flavia Cicuttini, Graeme Jones
OBJECTIVE: To describe the natural history of patellofemoral joint (PFJ) bone marrow lesions (BMLs) over 2.6 years and associations between change in PFJ BMLs, knee pain and knee cartilage morphology in older adults over 5 years. METHODS: Prospective population-based cohort study of men and women aged 50-80 years (mean age 63 years, n=406) was performed. PFJ BMLs, knee cartilage volume and cartilage defect scores (0-4) were measured using Whole-Organ Magnetic Resonance Imaging Score (WORMS) system at baseline and 2...
March 4, 2016: Arthritis Care & Research
https://www.readbyqxmd.com/read/26939775/associations-of-sarcopenic-obesity-and-dynapenic-obesity-with-bone-mineral-density-and-incident-fractures-over-5-10-years-in-community-dwelling-older-adults
#11
David Scott, Sahan D Chandrasekara, Laura L Laslett, Flavia Cicuttini, Peter R Ebeling, Graeme Jones
The purpose of this study is to determine whether low muscle mass (sarcopenia) or strength (dynapenia), in the presence of obesity, are associated with increased risk for osteoporosis and non-vertebral fracture over 5-10 years in community-dwelling older adults. N = 1089 volunteers (mean ± SD age 62 ± 7 years; 51 % female) participated at baseline and 761 attended follow-up clinics (mean 5.1 ± 0.5 years later). Total body, total hip and spine BMD, and appendicular lean and total fat mass were assessed by DXA...
July 2016: Calcified Tissue International
https://www.readbyqxmd.com/read/26919994/lower-limb-muscle-strength-is-associated-with-poor-balance-in-middle-aged-women-linear-and-nonlinear-analyses
#12
F Wu, M Callisaya, L L Laslett, K Wills, Y Zhou, G Jones, T Winzenberg
UNLABELLED: This was the first study investigating both linear associations between lower limb muscle strength and balance in middle-aged women and the potential for thresholds for the associations. There was strong evidence that even in middle-aged women, poorer LMS was associated with reduced balance. However, no evidence was found for thresholds. INTRODUCTION: Decline in balance begins in middle age, yet, the role of muscle strength in balance is rarely examined in this age group...
July 2016: Osteoporosis International
https://www.readbyqxmd.com/read/26767503/change-in-knee-structure-and-change-in-tibiofemoral-joint-space-width-a-five-year-longitudinal-population-based-study
#13
Joanna Hall, Laura L Laslett, Johanne Martel-Pelletier, Jean-Pierre Pelletier, François Abram, Chang-Hai Ding, Flavia M Cicuttini, Graeme Jones
BACKGROUND: Change in knee cartilage volume is frequently used as a proxy for change in knee joint space width over time, but longitudinal data on these associations is limited. We aimed to determine whether change in knee cartilage volume, new or worsening meniscal extrusion (ME), meniscal tears and cartilage defects over 2.4 years correlated with change in joint space width (JSW) over 5 years in older community dwelling adults. METHODS: Participants (n = 153) had their right knee imaged using MR imaging and x-ray at baseline, and after 2...
January 14, 2016: BMC Musculoskeletal Disorders
https://www.readbyqxmd.com/read/26596913/longitudinal-associations-between-adiposity-and-change-in-knee-pain-tasmanian-older-adult-cohort-study
#14
Xingzhong Jin, Changhai Ding, Xia Wang, Benny Antony, Laura L Laslett, Leigh Blizzard, Flavia Cicuttini, Graeme Jones
OBJECTIVE: The aim of this study was to describe the longitudinal relationship between adiposity and change in knee pain. METHODS: A total of 1099 participants aged 50-79 were randomly selected from the local community in Southern Tasmania, of which 767 were followed up on average 5.1 years later. Knee pain was assessed using the Western Ontario and McMaster Universities Osteoarthritis Index (WOMAC) at each time point. Consistent knee pain was defined as knee pain at all three time-points...
April 2016: Seminars in Arthritis and Rheumatism
https://www.readbyqxmd.com/read/26542095/american-college-of-cardiology-environmental-scanning-report-update-2015
#15
REVIEW
Lawrence Laslett, H Vernon Anderson, Bernard Clark, Timothy A Dewhurst, Thad F Waites, Sean Wilson, Steve Erickson, Anne Marie Smith
No abstract text is available yet for this article.
November 10, 2015: Journal of the American College of Cardiology
https://www.readbyqxmd.com/read/26465394/threshold-effects-of-vitamin-d-status-on-bone-health-in-chinese-adolescents-with-low-calcium-intake
#16
RANDOMIZED CONTROLLED TRIAL
Feitong Wu, Laura L Laslett, Qian Zhang
CONTEXT: There is no consensus on the definition of vitamin D deficiency for bone health based on serum 25-hydroxyvitamin D (25OHD) levels. OBJECTIVE: Our objective was to determine whether thresholds exist for associations between 25OHD levels and bone outcomes and if low 25OHD levels have adverse effects on bone health. DESIGN: This is a cross-sectional study. PARTICIPANTS: This study included secondary school students in Beijing, China, aged 12-15 years...
December 2015: Journal of Clinical Endocrinology and Metabolism
https://www.readbyqxmd.com/read/26304831/glycosyltransferase-st6gal1-contributes-to-the-regulation-of-pluripotency-in-human-pluripotent-stem-cells
#17
Yu-Chieh Wang, Jason W Stein, Candace L Lynch, Ha T Tran, Chia-Yao Lee, Ronald Coleman, Adam Hatch, Victor G Antontsev, Hun S Chy, Carmel M O'Brien, Shashi K Murthy, Andrew L Laslett, Suzanne E Peterson, Jeanne F Loring
Many studies have suggested the significance of glycosyltransferase-mediated macromolecule glycosylation in the regulation of pluripotent states in human pluripotent stem cells (hPSCs). Here, we observed that the sialyltransferase ST6GAL1 was preferentially expressed in undifferentiated hPSCs compared to non-pluripotent cells. A lectin which preferentially recognizes α-2,6 sialylated galactosides showed strong binding reactivity with undifferentiated hPSCs and their glycoproteins, and did so to a much lesser extent with differentiated cells...
2015: Scientific Reports
https://www.readbyqxmd.com/read/26276969/association-between-infrapatellar-fat-pad-volume-and-knee-structural-changes-in-patients-with-knee-osteoarthritis
#18
Jingyu Cai, Jianhua Xu, Kang Wang, Shuang Zheng, Fan He, Shuting Huan, Shengqing Xu, Hui Zhang, Laura Laslett, Changhai Ding
OBJECTIVE: The function of the infrapatellar fat pad (IPFP) in knee osteoarthritis (OA) remains uncertain. This study aimed to examine cross-sectional associations between IPFP volume and knee structures in patients with knee OA. METHODS: The study included 174 patients with clinical knee OA (mean age, 55.5 yrs). Fat-suppressed 3-D T1-weighted spoiled gradient recall magnetic resonance imaging (MRI) was used to measure the IPFP and cartilage volume. T2-weighted fast spin echo MRI was used to assess cartilage defects and bone marrow lesions (BML)...
October 2015: Journal of Rheumatology
https://www.readbyqxmd.com/read/25792491/changes-in-quality-of-life-associated-with-fragility-fractures-australian-arm-of-the-international-cost-and-utility-related-to-osteoporotic-fractures-study-ausicuros
#19
MULTICENTER STUDY
J Abimanyi-Ochom, J J Watts, F Borgström, G C Nicholson, C Shore-Lorenti, A L Stuart, Y Zhang, S Iuliano, E Seeman, R Prince, L March, M Cross, T Winzenberg, L L Laslett, G Duque, P R Ebeling, K M Sanders
UNLABELLED: We investigated change in health-related quality of life due to fracture in Australian adults aged over 50 years. Fractures reduce quality of life with the loss sustained at least over 12 months. At a population level, the loss was equivalent to 65 days in full health per fracture. PURPOSE: We aimed to quantify the change in health-related quality of life (HRQoL) that occurred as a consequence of a fracture using the EQ-5D-3 L questionnaire. METHODS: Adults aged ≥50 years with a low to moderate energy fracture were recruited from eight study centres across Australia...
June 2015: Osteoporosis International
https://www.readbyqxmd.com/read/25481426/lifestyle-modifications-to-improve-musculoskeletal-and-bone-health-and-reduce-disability-a-life-course-approach
#20
REVIEW
Graeme Jones, Tania M Winzenberg, Michele L Callisaya, Laura L Laslett
This review covers the evidence relating to lifestye modification in the big three musculoskeletal conditions: osteoarthritis, osteoporosis and rheumatoid arthritis. Lifestyle is of considerable importance in the first two and there is emerging evidence for rheumatoid arthritis despite it not traditionally being considered a lifestyle disease.
June 2014: Best Practice & Research. Clinical Rheumatology
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