keyword
MENU ▼
Read by QxMD icon Read
search

horizontal gene transfers

keyword
https://www.readbyqxmd.com/read/29350317/a-study-on-the-role-that-quorum-sensing-play-in-antibiotic-resistant-plasmid-conjugative-transfer-in-escherichia-coli
#1
Yueheng Zhang, Qingping Ma, Bingmei Su, Rui Chen, Juan Lin, Zhifen Lin, Dali Wang, Yang Yu
Horizontal genes transfer (HGT) plays an important role in the dissemination of antibiotic resistance genes (ARGs) in the environment. However, the mechanisms of HGT of ARGs under the influence of antibiotics in sub-MIC remain rarely explored. Moreover, given its collective nature, HGT was considered to be relative to quorum sensing (QS) system. To investigate whether QS has any impact on horizontal gene transfer of ARGs, experiments were conducted to determine the conjugative efficiency of plasmid RP4 on Escherichia coli (E...
January 19, 2018: Ecotoxicology
https://www.readbyqxmd.com/read/29350130/ecology-and-evolution-of-chromosomal-gene-transfer-between-environmental-microorganisms-and-pathogens
#2
José Luis Martínez
Inspection of the genomes of bacterial pathogens indicates that their pathogenic potential relies, at least in part, on the activity of different elements that have been acquired by horizontal gene transfer from other (usually unknown) microorganisms. Similarly, in the case of resistance to antibiotics, besides mutation-driven resistance, the incorporation of novel resistance genes is a widespread evolutionary procedure for the acquisition of this phenotype. Current information in the field supports the idea that most (if not all) genes acquired by horizontal gene transfer by bacterial pathogens and contributing to their virulence potential or to antibiotic resistance originate in environmental, not human-pathogenic, microorganisms...
January 2018: Microbiology Spectrum
https://www.readbyqxmd.com/read/29348510/genome-and-secretome-analysis-of-pochonia-chlamydosporia-provide-new-insight-into-egg-parasitic-mechanisms
#3
Runmao Lin, Feifei Qin, Baoming Shen, Qianqian Shi, Chichuan Liu, Xi Zhang, Yang Jiao, Jun Lu, Yaoyao Gao, Marta Suarez-Fernandez, Federico Lopez-Moya, Luis Vicente Lopez-Llorca, Gang Wang, Zhenchuan Mao, Jian Ling, Yuhong Yang, Xinyue Cheng, Bingyan Xie
Pochonia chlamydosporia infects eggs and females of economically important plant-parasitic nematodes. The fungal isolates parasitizing different nematodes are genetically distinct. To understand their intraspecific genetic differentiation, parasitic mechanisms, and adaptive evolution, we assembled seven putative chromosomes of P. chlamydosporia strain 170 isolated from root-knot nematode eggs (~44 Mb, including 7.19% of transposable elements) and compared them with the genome of the strain 123 (~41 Mb) isolated from cereal cyst nematode...
January 18, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29346651/bipartite-network-analysis-of-gene-sharings-in-the-microbial-world
#4
Eduardo Corel, Raphaël Méheust, Andrew K Watson, James O McInerney, Philippe Lopez, Eric Bapteste
Extensive microbial gene flows affect how we understand virology, microbiology, medical sciences, genetic modification and evolutionary biology. Phylogenies only provide a narrow view of these gene flows: plasmids and viruses, lacking core genes, cannot be attached to cellular life on phylogenetic trees. Yet viruses and plasmids have a major impact on cellular evolution, affecting both the gene content and the dynamics of microbial communities. Using bipartite graphs that connect up to 149,000 clusters of homologous genes with 8217 related and unrelated genomes, we can in particular show patterns of gene sharing that do not map neatly with the organismal phylogeny...
January 15, 2018: Molecular Biology and Evolution
https://www.readbyqxmd.com/read/29343426/environmental-mycobacterium-avium-subsp-hominissuis-have-a-higher-probability-to-act-as-a-recipient-in-conjugation-than-clinical-strains
#5
Salma A Shoulah, Anna M Oschmann, Abdelfattah Selim, Torsten Semmler, Carsten Schwarz, Elisabeth Kamal, Faysal Hamouda, Elsayed Galila, Wilbert Bitter, Astrid Lewin
Mycobacterium avium subsp. hominissuis (MAH) is a widespread opportunistic pathogen that can be isolated from environment (dust, soil and water) and patients with lung or lymphnode infection. In our previous research we revealed the pronounced genetic diversity in MAH by identifying eight different types of a newly described genomic island. In order to identify mechanisms of such horizontal gene transfer we now analyzed the ability of 47 MAH isolates to inherit the conjugative plasmid pRAW from M. marinum. A higher percentage of environmental isolates (22...
January 14, 2018: Plasmid
https://www.readbyqxmd.com/read/29337343/antimicrobial-resistance-genetic-markers-in-potentially-pathogenic-gram-positive-cocci-isolated-from-brazilian-soft-cheese
#6
Juliana Alves Resende, Cláudia Oliveira Fontes, Alessandra Barbosa Ferreira-Machado, Thiago César Nascimento, Vânia Lúcia Silva, Cláudio Galuppo Diniz
Although most Brazilian dairy products meet high technological standards, there are quality issues regarding milk production, which may reduce the final product quality. Several microbial species may contaminate milk during manufacture and handling. If antimicrobial usage remains uncontrolled in dairy cattle, the horizontal transfer of antimicrobial resistance genes in foodstuffs may be of particular concern for both food producers and dairy industry. This study focused on the evaluation of putative Gram positive cocci in Minas cheese and of antimicrobial and biocide resistance genes among the isolated bacteria...
January 16, 2018: Journal of Food Science
https://www.readbyqxmd.com/read/29333587/genetic-characterization-and-modification-of-a-bioethanol-producing-yeast-strain
#7
Ke Zhang, Ya-Nan Di, Lei Qi, Yang Sui, Ting-Yu Wang, Li Fan, Zhen-Mei Lv, Xue-Chang Wu, Pin-Mei Wang, Dao-Qiong Zheng
Yeast Saccharomyces cerevisiae strains isolated from different sources generally show extensive genetic and phenotypic diversity. Understanding how genomic variations influence phenotypes is important for developing strategies with improved economic traits. The diploid S. cerevisiae strain NY1308 is used for cellulosic bioethanol production. Whole genome sequencing identified an extensive amount of single nucleotide variations and small insertions/deletions in the genome of NY1308 compared with the S288c genome...
January 15, 2018: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/29330182/fluorescence-recovery-allows-the-implementation-of-a-fluorescence-reporter-gene-platform-applicable-for-the-detection-and-quantification-of-horizontal-gene-transfer-in-anoxic-environments
#8
Rafael Pinilla, Leise Riber, Søren J Sørensen
The study of horizontal gene transfer (HGT) in microbial communities has been revolutionized by significant advances in cultivation-independent methods based on fluorescence reporter gene technologies. Recently, the combination of these novel approaches with flow cytometry has presented itself as one of the most powerful tools to study the spread of mobile genetic elements (MGEs) in the environment. However, the use of fluorescent markers like green fluorescent protein (GFP) and mCherry is limited by environmental constraints that affect the correct maturation of their fluorophores, such as oxygen availability and pH levels...
January 12, 2018: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/29329601/nicotiana-glauca-whole-genome-investigation-for-ct-dna-study
#9
Galina Khafizova, Pavel Dobrynin, Dmitrii Polev, Tatiana Matveeva
OBJECTIVE: Nicotiana glauca (tree tobacco) is a naturally transgenic plant, containing sequences acquired from Agrobacterium rhizogenes by horizontal gene transfer. Besides, N. glauca contains a wide profile of alkaloids of medical interest. DATA DESCRIPTION: We report a high-depth sequencing and de novo assembly of N. glauca full genome and analysis of genome elements with bacterial origin. The draft genome assembly is 3.2 Gb, with N50 size of 31.1 kbp. Comparative analysis confirmed the presence of single, previously described gT insertion...
January 12, 2018: BMC Research Notes
https://www.readbyqxmd.com/read/29329439/conserved-proteins-of-the-rna-interference-system-in-the-arbuscular-mycorrhizal-fungus-rhizoglomus-irregulare-provide-new-insight-into-the-evolutionary-history-of-glomeromycota
#10
Soon-Jae Lee, Mengxuan Kong, Paul Harrison, Mohamed Hijri
Horizontal gene transfer (HGT) is an important mechanism in the evolution of many living organisms particularly in Prokaryotes where genes are frequently dispersed between taxa. Although, HGT has been reported in Eukaryotes, its accumulative effect and its frequency has been questioned. Arbuscular mycorrhizal fungi (AMF) are an early diverged fungal lineage belonging to phylum Glomeromycota, whose phylogenetic position is still under debate. The history of AMF and land plant symbiosis dates back to at least 460 million years ago...
January 10, 2018: Genome Biology and Evolution
https://www.readbyqxmd.com/read/29325123/packaging-of-dinoroseobacter-shibae-dna-into-gene-transfer-agent-particles-is-not-random
#11
Jürgen Tomasch, Hui Wang, April T K Hall, Diana Patzelt, Matthias Preusse, Jörn Petersen, Henner Brinkmann, Boyke Bunk, Sabin Bhuju, Michael Jarek, Robert Geffers, Andrew S Lang, Irene Wagner-Döbler
Gene transfer agents (GTAs) are phage-like particles which contain a fragment of genomic DNA of the bacterial or archaeal producer and deliver this to a recipient cell. GTA gene clusters are present in the genomes of almost all marine Rhodobacteraceae (Roseobacters) and might be important contributors to horizontal gene transfer (HGT) in the world's oceans. For all organisms studied so far, no obvious evidence of sequence specificity or other non-random process responsible for packaging genomic DNA into GTAs has been found...
January 9, 2018: Genome Biology and Evolution
https://www.readbyqxmd.com/read/29324288/description-of-an-orthologous-cluster-of-ochratoxin-a-biosynthetic-genes-in-aspergillus-and-penicillium-species-a-comparative-analysis
#12
Jessica Gil-Serna, Marta García-Díaz, María Teresa González-Jaén, Covadonga Vázquez, Belén Patiño
Ochratoxin A (OTA) is one of the most important mycotoxins due to its toxic properties and worldwide distribution which is produced by several Aspergillus and Penicillium species. The knowledge of OTA biosynthetic genes and understanding of the mechanisms involved in their regulation are essential. In this work, we obtained a clear picture of biosynthetic genes organization in the main OTA-producing Aspergillus and Penicillium species (A. steynii, A. westerdijkiae, A. niger, A. carbonarius and P. nordicum) using complete genome sequences obtained in this work or previously available on databases...
January 3, 2018: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/29322462/methods-to-determine-mutational-trajectories-after-experimental-evolution-of-antibiotic-resistance
#13
Douglas L Huseby, Diarmaid Hughes
The evolution of bacterial resistance to antibiotics by mutation within the genome (as distinct from horizontal gene transfer of new material into a genome) could occur in a single step but is usually a multistep process. Resistance evolution can be studied in laboratory environments by serial passage of bacteria in liquid culture or on agar, with selection at constant, or varying, concentrations of drug. Whole genome sequencing can be used to make an initial analysis of the evolved mutants. The trajectory of evolution can be determined by sequence analysis of strains from intermediate steps in the evolution, complemented by phenotypic analysis of genetically reconstructed isogenic strains that recapitulate the intermediate steps in the evolution...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29321570/transfer-of-a-bla-ctx-m-1-carrying-plasmid-between-different-escherichia-coli-strains-within-the-human-gut-explored-by-whole-genome-sequencing-analyses
#14
Per Kristian Knudsen, Karianne Wiger Gammelsrud, Kristian Alfsnes, Martin Steinbakk, Tore G Abrahamsen, Fredrik Müller, Jon Bohlin
Horizontal transfer of antibiotic resistance determinants contributes to dissemination of antibiotic resistance. Such transfer of resistance genes within the human gut has been documented in some in vivo studies. The present study investigated seven bla CTX-M-1-carrying Escherichia coli isolates from three consecutive faecal samples collected from one cystic fibrosis patient in a nine-months period, by analysing whole genome sequencing data. The analyses showed that the seven E. coli isolates represented three genetically diverse strains...
January 10, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29320853/high-co2-requirement-as-a-mechanism-for-the-containment-of-genetically-modified-cyanobacteria
#15
Ryan L Clark, Gina C Gordon, Nathaniel R Bennett, Haoxiang Lyu, Thatcher W Root, Brian F Pfleger
As researchers engineer cyanobacteria for biotechnological applications, we must consider potential environmental release of these organisms. Previous theoretical work has considered cyanobacterial containment through elimination of the CO2-concentrating mechanism (CCM) to impose a high-CO2 requirement (HCR), which could be provided in the cultivation environment but not in the surroundings. In this work, we experimentally implemented an HCR containment mechanism in Synechococcus sp. PCC7002 (PCC7002) through deletion of carboxysome shell proteins and showed that this mechanism contained cyanobacteria in a 5% CO2 environment...
January 10, 2018: ACS Synthetic Biology
https://www.readbyqxmd.com/read/29316517/functional-metagenomics-reveals-a-novel-carbapenem-hydrolyzing-mobile-beta-lactamase-from-indian-river-sediments-contaminated-with-antibiotic-production-waste
#16
Nachiket P Marathe, Anders Janzon, Stathis D Kotsakis, Carl-Fredrik Flach, Mohammad Razavi, Fanny Berglund, Erik Kristiansson, D G Joakim Larsson
Evolution has provided environmental bacteria with a plethora of genes that give resistance to antibiotic compounds. Under anthropogenic selection pressures, some of these genes are believed to be recruited over time into pathogens by horizontal gene transfer. River sediment polluted with fluoroquinolones and other drugs discharged from bulk drug production in India constitute an environment with unprecedented, long-term antibiotic selection pressures. It is therefore plausible that previously unknown resistance genes have evolved and/or are promoted here...
January 6, 2018: Environment International
https://www.readbyqxmd.com/read/29314736/development-of-a-chlamydia-suis-specific-antibody-enzyme-linked-immunosorbent-assay-based-on-the-use-of-a-b-cell-epitope-of-the-polymorphic-membrane-protein-c
#17
K De Puysseleyr, E Kieckens, L De Puysseleyr, H Van den Wyngaert, B Ahmed, S Van Lent, H H Creasy, G S A Myers, D Vanrompay
Chlamydia suis infections lead to economic loss in the pork industry. Chlamydia suis infections could be successfully treated with tetracyclines until the appearance of a tetracycline resistant phenotype, which was acquired via horizontal gene transfer of the tet(C) gene. Given the importance of C. suis as a swine pathogen and as a recently emerged tetracycline resistant pathogen with zoonotic potential, our aim was to develop a sensitive C. suis-specific antibody ELISA based on the polymorphic membrane proteins (Pmps)...
January 4, 2018: Transboundary and Emerging Diseases
https://www.readbyqxmd.com/read/29312823/a-historical-legacy-of-antibiotic-utilization-on-bacterial-seed-banks-in-sediments
#18
Laura Madueño, Christophe Paul, Thomas Junier, Zhanna Bayrychenko, Sevasti Filippidou, Karin Beck, Gilbert Greub, Helmut Bürgmann, Pilar Junier
The introduction of antibiotics for both medical and non-medical purposes has had a positive effect on human welfare and agricultural output in the past century. However, there is also an important ecological legacy regarding the use of antibiotics and the consequences of increased levels of these compounds in the environment as a consequence of their use and disposal. This legacy was investigated by quantifying two antibiotic resistance genes (ARG) conferring resistance to tetracycline (tet(W)) and sulfonamide (sul1) in bacterial seed bank DNA in sediments...
2018: PeerJ
https://www.readbyqxmd.com/read/29311273/translocation-through-the-conjugative-type-4-secretion-system-requires-unfolding-of-its-protein-substrate
#19
Martina Trokter, Gabriel Waksman
Bacterial conjugation, a mechanism of horizontal gene transfer, is the major means by which antibiotic resistance spreads among bacteria (1, 2). Conjugative plasmids are transferred from one bacterium to another through a type IV secretion system (T4SS) in a form of single-stranded DNA covalently attached to a protein called relaxase. The relaxase is fully functional both in a donor cell (prior to conjugation) and recipient cell (after conjugation). Here we demonstrate that the protein substrate has to unfold for efficient translocation through the conjugative T4SS...
January 8, 2018: Journal of Bacteriology
https://www.readbyqxmd.com/read/29311068/identification-of-a-novel-genomic-island-associated-with-vand-type-vancomycin-resistance-in-six-dutch-vancomycin-resistant-enterococcus-faecium
#20
Janetta Top, Jan C Sinnige, Ellen C Brouwer, Guido Werner, Jukka Corander, Juliëtte A Severin, Rogier Jansen, E Bathoorn, Marc J M Bonten, John W A Rossen, Rob J L Willems
Genomic comparison of the first six Dutch vanD-type vancomycin-resistant Enterococcus faecium (VRE) with four vanD-gene clusters from other enterococcal species and anaerobic gut commensals revealed that the vanD-gene cluster was located on a genomic island of variable size. Phylogenetic inferences revealed that the Dutch-VRE were genetically not closely related and genetic variation of the vanD-genomic island was not species specific, which suggest that this island is transferred horizontally between enterococci and anaerobic gut commensals...
January 8, 2018: Antimicrobial Agents and Chemotherapy
keyword
keyword
74168
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"