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itp and microrna

Ascensión M De Los Reyes-García, Ana B Arroyo, Raúl Teruel-Montoya, Vicente Vicente, María L Lozano, Rocío González-Conejero, Constantino Martínez
Although a growing number of studies suggest that microRNAs (miRNAs) play a relevant role in platelet biology, their implications in bleeding diatheses are starting to be investigated. Indeed, several studies have shown that alterations in the intracellular levels of highly expressed platelet miRNAs provoke a thrombotic phenotype. On the other hand, primary immune thrombocytopenia (ITP), which is considered the hallmark of acquired bleeding disorders, has been recently associated with altered levels of miRNAs in peripheral blood mononuclear cells, plasma, and platelets...
May 22, 2018: Platelets
Cheng Qian, Wenying Yan, Tengda Li, Qingya Cui, Peng Liu, Mingli Gu, Jie Guo, Weiwei Zhang, Chuanlu Ren, Tianqin Wu, Anmei Deng
BACKGROUND/AIMS: MicroRNAs (miRNAs) have been described to have important roles in primary immune thrombocytopenia (ITP). To gain additional understanding, we have now further evaluated the involvement of miRNAs in ITP. METHODS: Microarray experiments were performed to examine the expression profiles of miRNAs and mRNAs in samples from subjects with newly diagnosed ITP (G1), chronic ITP (G2), and normal controls. The systematic Pipeline of Outlier MicroRNA Analysis framework was applied to identify key miRNAs expressed in the G1 and G2 samples...
2018: Cellular Physiology and Biochemistry
Kuan-Chieh Tseng, Yi-Fan Chiang-Hsieh, Hsuan Pai, Chi-Nga Chow, Shu-Chuan Lee, Han-Qin Zheng, Po-Li Kuo, Guan-Zhen Li, Yu-Cheng Hung, Na-Sheng Lin, Wen-Chi Chang
Motivation: MicroRNAs (miRNAs) are endogenous non-coding small RNAs (of about 22 nucleotides), which play an important role in the post-transcriptional regulation of gene expression via either mRNA cleavage or translation inhibition. Several machine learning-based approaches have been developed to identify novel miRNAs from next generation sequencing (NGS) data. Typically, precursor/genomic sequences are required as references for most methods. However, the non-availability of genomic sequences is often a limitation in miRNA discovery in non-model plants...
April 1, 2018: Bioinformatics
Gang Deng, Shifang Yu, Yunlei He, Tao Sun, Wei Liang, Lu Yu, Deyi Xu, Qiang Li, Ri Zhang
Immune thrombocytopenia (ITP) is an autoimmune disease characterized by a low platelet count and insufficient platelet production. Previous studies identified that microRNAs (miRNAs/miRs) are important for platelet function. However, the regulatory role of miRNAs in the pathogenesis of thrombocytopenia in ITP remains unclear. The aim of the present study is to isolate differentially expressed miRNAs, and identify their roles in platelets from ITP. A total of 5 ml blood from 22 patients with ITP and 8 healthy controls was isolated for platelet collection...
September 2017: Molecular Medicine Reports
Bin Zuo, Juping Zhai, Lifang You, Yunxiao Zhao, Jianfeng Yang, Zhen Weng, Lan Dai, Qingyu Wu, Changgeng Ruan, Yang He
Altered microRNA (miRNA) expression has been reported in patients with immune thrombocytopenic purpura (ITP). However, the detailed expression profiling of cell-free circulating miRNAs in ITP patients has not been fully investigated. In this study, we aimed to examine plasma miRNAs in ITP patients and evaluate their diagnostic values. Plasma samples from 74 ITP patients and 58 healthy controls were obtained and allocated into discovery, validation, and therapy-response sets. Initial screen with a miRNA microarray assay identified 23 miRNAs with different levels between ITP patients and healthy controls (>1...
June 28, 2017: Thrombosis and Haemostasis
Lan Deng, Xiuju Wang, Ling Jiang, Jilong Yang, Xuan Zhou, Zhigang Lu, Haiyan Hu
Immune thrombocytopenia (ITP) is an acquired autoimmune disorder characterized by low platelet count and bleeding, and is usually triggered by viral infections. We previously reported that 14 viral microRNAs of megakaryocytes cultured with serum from patients with ITP, including ebv-miR-BART6, are up-regulated. Previous research has reported that ebv-miR-BART6 down-regulated the expression of miR-185-5p. We therefore predicted that the ABCG4 gene, which is highly expressed in megakaryocyte progenitor cells, is a direct target of miR-185-5p...
December 2016: International Journal of Biochemistry & Cell Biology
Lu Liu, Mingqiang Hua, Chuanfang Liu, Na He, Zhao Li, Daoxin Ma
Both microRNAs and T helper (Th) cells involve in autoimmune diseases and their effects and interactions in immune thrombocytopenia (ITP) remain unclear. In the present study, we investigated the expression profiles of seven immune-related microRNAs (miR-155, 146a, 326, 142-3p, 17-5p, 21 and 181a) and the frequencies of four Th cells (Th1, Th2, Th17 and Treg) in peripheral blood mononuclear cell (PBMCs) of ITP patients and healthy controls. Platelet autoantibodies specific for GPIIb/IIIa or GPIb/IX were measured using MAIPA method...
November 22, 2016: Oncotarget
Jian-Qin Li, Shao-Yan Hu, Zhao-Yue Wang, Jing Lin, Su Jian, Yong-Chao Dong, Xiao-Fang Wu, Dai-Lan, Li-Juan Cao
BACKGROUND: The imbalance of Treg/Th17 cells is an important pathogenic factor for immune thrombocytopenic purpura (ITP). We previously reported miR-125a-5p targeted CXCL13 and participated in the process of ITP. In the present study, the role of miR-125a-5p in regulating Treg/Th17 ratio and its potential molecular mechanism were investigated. METHOD: A total of 30 adults with ITP and 30 healthy subjects were included. MEG3 expression in peripheral blood derived CD4+ T cells from ITP patients and healthy subjects were detected by real-time PCR...
October 2016: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
Y Guo, W Qu, Y H Wang, H Liu, L J Li, H Q Wang, R Fu, H Liu, Y H Wu, J Guan, L M Xing, Z H Shao
OBJECTIVE: To explore the role of microRNA-155 (miR-155) in pathogenesis of immune thrombocytopenia (ITP) through investigate the relevance between the expression of miR-155 in CD19(+) B cells in peripheral blood and the function of B lymphocytes in patients with ITP. METHODS: A total of 55 ITP patients (30 newly diagnosed and 25 in remission) were collected from December 2014 to October 2015 in Tianjin Medical University General Hospital, and 25 healthy volunteers were recruited as controls...
April 12, 2016: Zhonghua Yi Xue za Zhi [Chinese medical journal]
Burenbatu, Mandula Borjigin, Eerdunduleng, Wenyan Huo, Cuiqin Gong, Hasengaowa, Guiping Zhang, Longmei, Ming Li, Xuemei Zhang, Xiaohui Sun, Jie Yang, Shuanglian Wang, Narisu Narisu, Yangjian Liu, Haihua Bai
Immune thrombocytopenia (ITP), also known as idiopathic thrombocytopenic purpura, is an autoimmune disease characterized by low platelet count and increased bleeding tendency. Currently, glucocorticoid and splenectomy are the main therapies for ITP but with obvious side effects including tendency of relapse and risk of internal bleeding. In this study, we report the Mongolian medicine Qishunbaolier (QSBLE) can significantly and efficiently increase platelet count with a low recurrent rate and unnoticeable side effect...
October 2015: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
Jian-Qin Li, Shao-Yan Hu, Zhao-Yue Wang, Jing Lin, Su Jian, Yong-Chao Dong, Xiao-Fang Wu, Dai Lan, Li-Juan Cao
BACKGROUND: Immune thrombocytopenia (ITP) is an acquired and autoimmune disease of adults and children characterized by decreased platelet production. CXC chemokine ligand-13 (CXCL13) participates in multiple immunological responses. However, it is still unknown the relationship between CXCL13 and ITP. METHODS: Plasma CXCL13 was detected in ITP (n = 30) children. CD4+ T cells was isolated from peripheral blood mononuclear cells (PBMCs) from healthy volunteers. Treated CD4+ T cells with dexamethasone and/or miR-125-5p mimic/inhibitor, to observe the regulation of CXCL13...
2015: American Journal of Translational Research
Chia-Hung Chien, Yi-Fan Chiang-Hsieh, Yi-An Chen, Chi-Nga Chow, Nai-Yun Wu, Ping-Fu Hou, Wen-Chi Chang
Compared with animal microRNAs (miRNAs), our limited knowledge of how miRNAs involve in significant biological processes in plants is still unclear. AtmiRNET is a novel resource geared toward plant scientists for reconstructing regulatory networks of Arabidopsis miRNAs. By means of highlighted miRNA studies in target recognition, functional enrichment of target genes, promoter identification and detection of cis- and trans-elements, AtmiRNET allows users to explore mechanisms of transcriptional regulation and miRNA functions in Arabidopsis thaliana, which are rarely investigated so far...
2015: Database: the Journal of Biological Databases and Curation
David W Wegman, Farhad Ghasemi, Anna Khorshidi, Burton B Yang, Stanley K Liu, George M Yousef, Sergey N Krylov
Sets of deregulated microRNAs (miRNAs), termed miRNA signatures, are promising biomarkers for cancer. Validation of miRNA signatures requires a technique that is accurate, sensitive, capable of detecting multiple miRNAs, fast, robust, and not cost-prohibitive. Direct quantitative analysis of multiple miRNAs (DQAMmiR) is a capillary electrophoresis (CE)-based hybridization assay that was suggested as a methodological platform for validation and clinical use of miRNA signatures. While satisfying the other requirements, DQAMmiR is not sufficiently sensitive to detect low-abundance miRNAs...
January 20, 2015: Analytical Chemistry
Bao-Hua Qian, Xin Ye, Lei Zhang, Yi Sun, Jian-Rong Zhang, Ming-Li Gu, Qin Qin, Jie Chen, An-Mei Deng
We investigated the possible pathogenic role of a microRNA (miR-155) in primary immune thrombocytopenia (ITP). We used quantitative real-time PCR to determine the relative expression of miR-155 and SOCS1 (suppressor of cytokine signaling) mRNA in peripheral blood mononuclear cells (PBMCs) from 28 ITP patients and 28 healthy controls. Cytokine plasma levels were determined by ELISA. Possible associations between miR-155 levels and serum cytokine concentrations were assessed using Spearman or Pearson correlation analysis...
2015: Acta Haematologica
Tao Sui, Li Ma, Xin Li, Qing Li, Xue Bai, Juan Mu, Mingfeng Zhao
OBJECTIVE: To screen the plasma microRNA (miRNA) profile of immune thrombocytopenia (ITP) patients. METHODS: Agilent 19.0 miRNA microarray was used to detect the expression profile of miRNA in plasma from 25 ITP patients and 20 healthy controls from June 2012 to September 2013. The software programs of TargetScan and miRanda were used for predicting target genes associated with differential miRNA. Then gene ontology (GO) and pathway analysis were performed to explore the genes and pathways involved in the pathogenesis of ITP...
April 15, 2014: Zhonghua Yi Xue za Zhi [Chinese medical journal]
Haifeng Zhao, Huiyuan Li, Weiting Du, Donglei Zhang, Jing Ge, Feng Xue, Zeping Zhou, Renchi Yang
MicroRNAs (miRNAs) play a vital role in the regulation of immunological functions and prevention of autoimmune disease. The abnormal expressions of several miRNAs in patients with the acquired autoimmune disease, immune thrombocytopenia (ITP), have been reported. However, the exact mechanism of miRNAs in the pathogenesis of ITP is currently not well understood. This study examined the miRNA expression profile of peripheral blood mononuclear cells (PBMCs) in ITP patients by miRNA array and TaqMan real-time polymerase chain reaction...
September 2014: British Journal of Haematology
Haifeng Zhao, Yizhuo Zhang, Feng Xue, Jianfen Xu, Zhi Fang
The purpose of this study was to determine the association of single nucleotide polymorphisms (SNP) of the has-mir-146a (miR-146a) genes with the risk for immune thrombocytopenia (ITP). The genotyping of miR-146a rs2910164 polymorphism was detected by polymerase chain reaction-restriction fragment length polymorphism. In the patients with ITP, the frequencies of GG, GC and CC genotypes and G and C alleles were 12.5%, 47.9%, 39.6%, 36.4% and 63.6%, respectively. There was no significant difference in genotype and alleles distribution between the ITP patient and the controls (p = 0...
May 2014: Autoimmunity
Ali Bay, Enes Coskun, Serdar Oztuzcu, Sercan Ergun, Fatih Yilmaz, Elif Aktekin
Immune thrombocytopenic purpura (ITP) is a commonly acquired autoimmune bleeding disorder in children. MicroRNAs (miRNAs) are small RNAs which are found in cells and circulation, and play a role in protein synthesis and regulation. In this study, we aimed to determine a biomarker for childhood ITP comparing the plasma miRNA levels of children having ITP with healthy children. A total of 86 patients with ITP and 56 healthy children followed up by the Department of Pediatric Hematology and Oncology in University of Gaziantep since July 2011 were enrolled in the study...
June 2014: Blood Coagulation & Fibrinolysis: An International Journal in Haemostasis and Thrombosis
Huiyuan Li, Haifeng Zhao, Feng Xue, Xian Zhang, Donglei Zhang, Jing Ge, Yanhui Yang, Min Xuan, Rongfeng Fu, Renchi Yang
Primary immune thrombocytopenia (ITP) is an acquired autoimmune disease with many immune dysfunctions. MicroRNAs (miRNAs) are a class of non-coding RNAs that post-transcriptionally regulate gene expression by messenger RNA degradation or translational repression. Accumulating evidence has demonstrated that miRNAs play a vital role in the regulation of immunological functions and prevention of autoimmunity. However, whether miRNAs are involved in the pathogenesis of ITP is still unknown. To illustrate the role of miRNAs in ITP, the expression profile of miRNAs from peripheral blood mononuclear cells (PBMCs) in ITP patients was investigated by miRNA microarray, and further validated by TaqMan real-time polymerase chain reaction...
April 2013: British Journal of Haematology
Margareta Jernås, Intawat Nookaew, Hans Wadenvik, Bob Olsson
MicroRNA are small noncoding RNA molecules that regulate gene expression. To investigate the role of microRNA in immune thrombocytopenia (ITP), we performed genome-wide expression analyses of mRNA and microRNA in T cells from ITP patients and controls. We identified 1915 regulated genes and 22 regulated microRNA that differed between ITP patients and controls. Seventeen of the 22 regulated microRNA were linked to changes in target gene expression; 57 of these target genes were associated with the immune system, eg, T-cell activation and regulation of immunoglobulin production...
March 14, 2013: Blood
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