Parnian Doostdar, Joshua Hawley, Kunal Chopra, Elli Marinopoulou, Robert Lea, Kiana Arashvand, Veronica Biga, Nancy Papalopulu, Ximena Soto Rodriguez
We investigate the effect of altering the protein expression dynamics of the bHLH transcription factor Her6 at the single-cell level in the embryonic zebrafish telencephalon. Using an homozygote endogenous Her6:Venus reporter and 4D single-cell tracking, we show that Her6 oscillates in neural telencephalic progenitors and that fusion of protein destabilisation domain (PEST) alters its expression dynamics, causing most cells to downregulate Her6 prematurely. However, counterintuitively, oscillatory cells increase with some expressing Her6 at high levels, resulting in increased heterogeneity of Her6 expression in the population...
April 26, 2024: Development