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leucine metabolite

Hailong Zhang, Longzhen Cui, Wen Liu, Zhenfeng Wang, Yang Ye, Xue Li, Huijuan Wang
Introduction: Gastric cancer (GC) is a malignant tumor worldwide. As primary pathway for metastasis, the lymphatic system is an important prognostic factor for GC patients. Although the metabolic changes of gastric cancer have been investigated in extensive studies, little effort focused on the metabolic profiling of lymph node metastasis (LNM)-positive or negative GC patients. Objectives: We performed1 H NMR spectrum of GC tissue samples with and without LNM to identify novel potential metabolic biomarkers in the process of LNM of GC...
2018: Metabolomics: Official Journal of the Metabolomic Society
Nicolaia Iaffaldano, Michele Di Iorio, Luisa Mannina, Gianluca Paventi, Maria Pina Rosato, Silvia Cerolini, Anatoly P Sobolev
Metabolic profile of fresh turkey spermatozoa at three different reproductive period ages, namely 32, 44 and 56 weeks, was monitored by Nuclear Magnetic Resonance (NMR) spectroscopy and correlated to sperm quality parameters. The age-related decrease in sperm quality as indicated by reduction of sperm concentration, sperm mobility and osmotic tolerance was associated to variation in the level of specific water-soluble and liposoluble metabolites. In particular, the highest levels of isoleucine, phenylalanine, leucine, tyrosine and valine were found at 32 weeks of age, whereas aspartate, lactate, creatine, carnitine, acetylcarnitine levels increased during the ageing...
2018: PloS One
Andréa T Faccio, Francisco J Ruperez, Nagendra S Singh, Santiago Angulo, Marina F M Tavares, Michel Bernier, Coral Barbas, Irving W Wainer
BACKGROUND: Impairment in mitochondrial biogenesis and function plays a key role in depression and anxiety, both of which being associated with changes in fatty acid and phospholipid metabolism. The antidepressant effects of (R,S)-ketamine have been linked to its conversion into (2S,6S;2R,6R)-hydroxynorketamine (HNK); however, the connection between structure and stereochemistry of ketamine and HNK in the mitochondrial homeostatic response has not yet been fully elucidated at a metabolic level...
March 8, 2018: Biochimica et Biophysica Acta
Juho Tynkkynen, Vincent Chouraki, Sven J van der Lee, Jussi Hernesniemi, Qiong Yang, Shuo Li, Alexa Beiser, Martin G Larson, Katri Sääksjärvi, Martin J Shipley, Archana Singh-Manoux, Robert E Gerszten, Thomas J Wang, Aki S Havulinna, Peter Würtz, Krista Fischer, Ayse Demirkan, M Arfan Ikram, Najaf Amin, Terho Lehtimäki, Mika Kähönen, Markus Perola, Andres Metspalu, Antti J Kangas, Pasi Soininen, Mika Ala-Korpela, Ramachandran S Vasan, Mika Kivimäki, Cornelia M van Duijn, Sudha Seshadri, Veikko Salomaa
INTRODUCTION: Metabolite, lipid, and lipoprotein lipid profiling can provide novel insights into mechanisms underlying incident dementia and Alzheimer's disease. METHODS: We studied eight prospective cohorts with 22,623 participants profiled by nuclear magnetic resonance or mass spectrometry metabolomics. Four cohorts were used for discovery with replication undertaken in the other four to avoid false positives. For metabolites that survived replication, combined association results are presented...
February 28, 2018: Alzheimer's & Dementia: the Journal of the Alzheimer's Association
Caiyun Fan, Di Su, He Tian, Xiaojiao Li, Yu Li, Lei Ran, Ruiting Hu, Jianbo Cheng
Objective: The objective of the present study was to elucidate the mechanism underlying liver metabolic perturbations in dairy cows exposed to heat stress (HS). Method: Liquid chromatography mass spectrometry (LC-MS) was used to analyze metabolic differences in livers of 20 dairy cows, with and without exposure to HS. Results: The results revealed 33 potential metabolite candidate biomarkers for the detection of HS in dairy cows. Fifteen of these metabolites (glucose, lactate, pyruvate, acetoacetate, β-hydroxybutyrate (BHBA), fumaric acid, citric acid, choline, glycine, proline, isoleucine, leucine, urea, creatinine, and orotic acid) were previously found to be potential biomarkers of HS in plasma or milk, discriminating dairy cows with and without HS...
March 2, 2018: Asian-Australasian Journal of Animal Sciences
Sun Ha Jee, Minjoo Kim, Minkyung Kim, Hye Jin Yoo, Hyungyoon Kim, Keum Ji Jung, Seri Hong, Jong Ho Lee
In the prospective Korean Cancer Prevention Study-II (KCPS-II), we investigated the application of metabolomics to differentiate subjects with incident hepatocellular carcinoma (HCC group) from subjects who remained free of cancer (control group) during a mean follow-up period of 7 years with the aim of identifying valuable metabolic biomarkers for HCC. We used baseline serum samples from 75 subjects with incident HCC and 134 age- and gender-matched cancer-free subjects. Serum metabolic profiles associated with HCC incidence were investigated via metabolomics analysis...
March 2, 2018: Cancer Prevention Research
Soo Jin Yang, Hyun Ju Do, Youngae Jung, Geum-Sook Hwang, Min-Jeong Shin
The alpha-glucosidase inhibitor voglibose (VO) was recently reported to have a protective effect against weight gain as well as affect various metabolic changes related to food intake and gut-brain signaling. We hypothesized that VO prevents weight gain by altering neurometabolome profiles in the hypothalamus to reduce food intake. To test this hypothesis, we assessed metabolite profiles in the hypothalamus of standard diet- or high-fat (HF) diet-fed mice in the absence or presence of VO. In total, 29 male C57BL/6 mice were divided into 3 groups: (1) lean control, (2) HF, and (3) HF + VO...
March 1, 2018: Nutritional Neuroscience
Yanyun Li, Minjian Chen, Cuiping Liu, Yankai Xia, Bo Xu, Yanhui Hu, Ting Chen, Meiping Shen, Wei Tang
Papillary thyroid carcinoma (PTC) is the most common thyroid cancer. Nuclear magnetic resonance (NMR)‑based metabolomic technique is the gold standard in metabolite structural elucidation, and can provide different coverage of information compared with other metabolomic techniques. Here, we firstly conducted NMR based metabolomics study regarding detailed metabolic changes especially metabolic pathway changes related to PTC pathogenesis. 1H NMR-based metabolomic technique was adopted in conju-nction with multivariate analysis to analyze matched tumor and normal thyroid tissues obtained from 16 patients...
February 16, 2018: International Journal of Molecular Medicine
Gerard Bryan Gonzales, Sarah De Saeger
In this paper, the stability of the plasma metabolome at -20 °C for up to 30 days was evaluated using liquid chromatography-high resolution mass spectrometric metabolomics analysis. To follow the time-series deterioration of the plasma metabolome, the use of an elastic net regularized regression model for the prediction of storage time at -20 °C based on the plasma metabolomic profile, and the selection and ranking of metabolites with high temporal changes was demonstrated using the glmnet package in R...
February 26, 2018: Scientific Reports
Tanya M Teslovich, Daniel Seung Kim, Xianyong Yin, Alena Stancáková, Anne U Jackson, Matthias Wielscher, Adam Naj, John R B Perry, Jeroen R Huyghe, Heather M Stringham, James P Davis, Chelsea K Raulerson, Ryan P Welch, Christian Fuchsberger, Adam E Locke, Xueling Sim, Peter S Chines, Narisu Narisu, Antti J Kangas, Pasi Soininen, Mika Ala-Korpela, Vilmundur Gudnason, Solomon K Musani, Marjo-Riitta Jarvelin, Gerard D Schellenberg, Elizabeth K Speliotes, Johanna Kuusisto, Francis S Collins, Michael Boehnke, Markku Laakso, Karen L Mohlke
Comprehensive metabolite profiling captures many highly heritable traits, including amino acid levels, which are potentially sensitive biomarkers for disease pathogenesis. To better understand the contribution of genetic variation to amino acid levels, we performed single variant and gene-based tests of association between nine serum amino acids (alanine, glutamine, glycine, histidine, isoleucine, leucine, phenylalanine, tyrosine, and valine) and 16.6 million genotyped and imputed variants in 8,545 non-diabetic Finnish men from the METabolic Syndrome In Men (METSIM) study...
February 21, 2018: Human Molecular Genetics
Steven D Melvin, Oliver A H Jones, Anthony R Carroll, Frederic D L Leusch
Organic carrier solvents are used in aquatic toxicity testing to improve chemical solubility and facilitate the exploration of dose-response relationships. Both water- and solvent-control groups are normally included in these scenarios to ensure that the solvent itself has no effect on the test organism, but this fails to consider possible interactive effects between carrier solvents and contaminants of interest. We explored this topic by exposing Limnodynastes peronii tadpoles to a mixture of common water-soluble pharmaceuticals (diclofenac, metformin and valproic acid) in the presence and absence of the carrier solvent methanol, according to standard developmental bioassay methodology...
February 10, 2018: Chemosphere
Stéphanie Leruez, Thomas Bresson, Juan M Chao de la Barca, Alexandre Marill, Grégoire de Saint Martin, Adrien Buisset, Jeanne Muller, Lydie Tessier, Cédric Gadras, Christophe Verny, Patrizia Amati-Bonneau, Guy Lenaers, Philippe Gohier, Dominique Bonneau, Gilles Simard, Dan Milea, Vincent Procaccio, Pascal Reynier
Purpose: To determine the plasma metabolomic signature of the exfoliative syndrome (XFS), the most common cause worldwide of secondary open-angle glaucoma. Methods: We performed a targeted metabolomic study, using the standardized p180 Biocrates Absolute IDQ p180 kit with a QTRAP 5500 mass spectrometer, to compare the metabolomic profiles of plasma from individuals with XFS (n = 16), and an age- and sex-matched control group with cataract (n = 18). Results: A total of 151 metabolites were detected correctly, 16 of which allowed for construction of an OPLS-DA model with a good predictive capability (Q2cum = 0...
February 1, 2018: Investigative Ophthalmology & Visual Science
Ju Li, Nan Che, Lingxiao Xu, Qian Zhang, Qi Wang, Wenfeng Tan, Miaojia Zhang
Metabolomics has been applied to explore altered metabolite profiles in disease and identify unique metabolic signatures in recent years. We aim to characterize the metabolic profile of rheumatoid arthritis patients and explore its underlying pathological processes using metabolomics approach. Serum samples from 30 rheumatoid arthritis (RA) patients, 30 primary Sjogren's syndrome (pSS) patients, and 32 healthy controls (HC) were collected. The sample was analyzed by ultra-high-performance liquid chromatography coupled with high-resolution mass spectrometry (UPLC-HRMS)...
February 13, 2018: Clinical Rheumatology
Hyang-Gi Ji, Yeong-Ran Lee, Min-Seuk Lee, Kyeong-Hwan Hwang, Clara Yongjoo Park, Eun-Hee Kim, Jun Seong Park, Young-Shick Hong
With increase of tea (Camellia sinensis) consumption, its chemical or metabolite compositions play a crucial role in the determination of tea quality. In general, metabolite compositions of fresh tea leaves including shoots depend on plucking seasons and tea cultivators. Therefore, choosing specific plucking time of tea leaves can provide use-specified tea products. Artificial control of tea growing, typically shade treatments, can lead to significant changes of the tea metabolite compositions of tea. However, metabolic characteristics of tea grown under various shade treatments conditions remain unclear...
February 6, 2018: Journal of Agricultural and Food Chemistry
Alberto García-Villaescusa, José Manuel Morales-Tatay, Daniel Monleón-Salvadó, José Manuel González-Darder, Carlos Bellot-Arcis, José María Montiel-Company, José Manuel Almerich-Silla
Nowadays there is increasing interest in identifying-and using-metabolites that can be employed as biomarkers for diagnosing, treating and monitoring diseases. Saliva and NMR have been widely used for this purpose as they are fast and inexpensive methods. This case-control study aimed to find biomarkers that could be related to glioblastoma (GBL) and periodontal disease (PD) and studied a possible association between GBL and periodontal status. The participants numbered 130, of whom 10 were diagnosed with GBL and were assigned to the cases group, while the remaining 120 did not present any pathology and were assigned to the control group...
2018: PloS One
Mariëlle P K J Engelen, Nicolaas E P Deutz
PURPOSE OF REVIEW: β-Hydroxy β-methylbutyrate (HMB) has been used for many years in athletes for muscle buildup and strength, and endurance enhancement. In recent years, its interest quickly expanded in older (diseased) populations and during (exercise) rehabilitation and recovery from hospitalization and surgery. We will discuss recent literature about HMB metabolism, its pharmacokinetics compared with the frequently used metabolite leucine, effectiveness of HMB to improve outcome in older diseased adults, and novel approaches for HMB use...
February 5, 2018: Current Opinion in Clinical Nutrition and Metabolic Care
Claudia Grajeda-Iglesias, Oren Rom, Shadi Hamoud, Nina Volkova, Tony Hayek, Niroz Abu-Saleh, Michael Aviram
Whereas atherogenicity of dietary lipids has been largely studied, relatively little is known about the possible contribution of dietary amino acids to macrophage foam-cell formation, a hallmark of early atherogenesis. Recently, we showed that leucine has antiatherogenic properties in the macrophage model system. In this study, an in-depth investigation of the role of leucine in macrophage lipid metabolism was conducted by supplementing humans, mice, or cultured macrophages with leucine. Macrophage incubation with serum obtained from healthy adults supplemented with leucine (5 g/d, 3 weeks) significantly decreased cellular cholesterol mass by inhibiting the rate of cholesterol biosynthesis and increasing cholesterol efflux from macrophages...
February 5, 2018: BioFactors
Kuo Gao, Ran Yang, Jian Zhang, Zhiyong Wang, Caixia Jia, Feilong Zhang, Shaojing Li, Jinping Wang, Ghulam Murtaza, Hua Xie, Huihui Zhao, Wei Wang, Jianxin Chen
Qijian mixture, a new traditional Chinese medicine (TCM) formula comprising of Astragalus membranaceus, Ramulus euonymi, Coptis chinensis and Pueraria lobata, was designed to ameliorate the type 2 diabetes (T2D), and its safety and efficacy were evaluated in the research by metabonomics, gut microbiota and system pharmacology. To study the hypoglycemic effect of Qijian mixture, male KKay mice (28-30 g, 8-9 week) and C57/BL6 mice (18-19 g, 8-9 week) were used. Thirty KKay diabetic mice were randomly distributed into 5 groups, abbreviated as Model group (Model), Low Qijian Mixture group (QJM(L)), High Qijian Mixture group (QJM(H)), Chinese Medicine (Gegen Qinlian Decoction) Positive group (GGQL), and Western Medicine (Metformin hydrochloride) Positive group (Metformin)...
January 29, 2018: Pharmacological Research: the Official Journal of the Italian Pharmacological Society
Xiaozheng Du, Bo Yuan, Jinhai Wang, Xinghua Zhang, Liang Tian, Tingzhuo Zhang, Xinglan Li, Fengfan Zhang
OBJECTIVE: To explore heat-reinforcing needling for the metabolite profiling changes in serum of rheumatoid arthritis (RA) rabbits with liquid chromatograph-mass spectrometer (LC-MS) technique, and to investigate its mechanisms. METHODS: Forty clean purple blue rabbits were randomized into a normal group, a model group, a reinforcing-reducing needling (RRN) group, a twirling-reinforcing needling (TRN) group, and a heat-reinforcing needling (HRN) group, 8 cases in each group...
September 12, 2017: Zhongguo Zhen Jiu, Chinese Acupuncture & Moxibustion
Noora Houttu, Kati Mokkala, Kirsi Laitinen
BACKGROUND: Overweight and obesity may predispose women to clinical complications during their pregnancy. We hypothesize that a higher degree of overweight status is related to a range of aberrations in biomarkers already in early pregnancy. Our objective was to investigate whether intestinal microbiota, serum metabolic and inflammatory profiles differ in relation to the degree of overweight status in pregnant women. METHODS: This study investigated 52 overweight and 47 obese pregnant women in early pregnancy...
December 27, 2017: Clinical Nutrition: Official Journal of the European Society of Parenteral and Enteral Nutrition
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