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https://www.readbyqxmd.com/read/28928660/-1-h-nmr-based-metabolomics-study-of-the-toxicological-effects-in-rats-induced-by-renqing-mangjue-pill-a-traditional-tibetan-medicine
#1
Can Xu, Caidan Rezeng, Jian Li, Lan Zhang, Yujing Yan, Jian Gao, Yingfeng Wang, Zhongfeng Li, Jianxin Chen
"RenqingMangjue" pill (RMP), as an effective prescription of Traditional Tibetan Medicine (TTM), has been widely used in treating digestive diseases and ulcerative colitis for over a thousand years. In certain classical Tibetan Medicine, heavy metal may add as an active ingredient, but it may cause contamination unintentionally in some cases. Therefore, the toxicity and adverse effects of TTM became to draw public attention. In this study, 48 male Wistar rats were orally administrated with different dosages of RMP once a day for 15 consecutive days, then half of the rats were euthanized on the 15th day and the remaining were euthanized on the 30th day...
2017: Frontiers in Pharmacology
https://www.readbyqxmd.com/read/28926946/the-fungal-bzip-transcription-factor-atfb-controls-virulence-associated-processes-in-aspergillus-parasiticus
#2
Josephine Wee, Sung-Yong Hong, Ludmila V Roze, Devin M Day, Anindya Chanda, John E Linz
Fungal basic leucine zipper (bZIP) transcription factors mediate responses to oxidative stress. The ability to regulate stress response pathways in Aspergillus spp. was postulated to be an important virulence-associated cellular process, because it helps establish infection in humans, plants, and animals. Previous studies have demonstrated that the fungal transcription factor AtfB encodes a protein that is associated with resistance to oxidative stress in asexual conidiospores, and AtfB binds to the promoters of several stress response genes...
September 16, 2017: Toxins
https://www.readbyqxmd.com/read/28923651/high-resolution-magic-angle-spinning-nuclear-magnetic-resonance-spectroscopy-for-the-metabolic-assessment-of-acute-rejection-after-cardiac-transplantation-in-rats
#3
C W Lee, J S Lee, C W Woo, S Kim
PURPOSE: To evaluate the potential of high-resolution magic angle spinning (HR-MAS) (1)H nuclear magnetic resonance (NMR) spectroscopy for metabolite characterization and the differentiation of acute rejection after heart transplantation in rat models. METHODS: We transplanted syngeneic heart grafts from Lewis rats (n = 4) and allogeneic heart grafts from F344 rats (n = 4) heterotopically into Lewis recipients. On day 7 postoperatively, the transplanted hearts were harvested for ex vivo (1)H NMR spectroscopy and HR-MAS (1)H NMR spectroscopy...
October 2017: Transplantation Proceedings
https://www.readbyqxmd.com/read/28915534/revealing-anti-inflammation-mechanism-of-water-extract-and-oil-of-forsythiae-fructus-on-carrageenan-induced-edema-rats-by-serum-metabolomics
#4
An Yuan, Lihong Gong, Lin Luo, Jue Dang, Xiaohong Gong, Mengjie Zhao, Yan Li, Yunxia Li, Cheng Peng
Forsythiae Fructus is an important Chinese medicine which shows a significant effect against inflammation. This study aimed to investigate the preventive anti-inflammation mechanism of Forsythiae Fructus by serum metabolomics strategy and compare the difference of the metabolism pathways between Forsythia extract and Forsythia oil in rat. Four groups (control group, model group, Forsythia extract group and Forsythia oil group) were orally administered 10mL/kg 0.5% Tween 80 solution, 10mL/kg 0.5% Tween 80 solution, 5g/kg Forsythia extract and 0...
September 11, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28904371/acute-interaction-between-hydrocortisone-and-insulin-alters-the-plasma-metabolome-in-humans
#5
Mohammad A Alwashih, Roland H Stimson, Ruth Andrew, Brian R Walker, David G Watson
With the aim of identifying biomarkers of glucocorticoid action and their relationship with biomarkers of insulin action, metabolomic profiling was carried out in plasma samples from twenty healthy men who were administered either a low or medium dose insulin infusion (n = 10 each group). In addition, all subjects were given metyrapone (to inhibit adrenal cortisol secretion) + /- hydrocortisone (HC) in a randomised crossover design to produce low, medium and high glucocorticoid levels. The clearest effects of insulin were to reduce plasma levels of the branched chain amino acids (BCAs) leucine/isoleucine and their deaminated metabolites, and lowered free fatty acids and acylcarnitines...
September 13, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28901489/uplc%C3%A2-qtofms%C3%A2-based-metabolomic-analysis-of-the-serum-of-hypoxic-preconditioning-mice
#6
Jie Liu, Gang Zhan, Dewei Chen, Jian Chen, Zhi-Bin Yuan, Er-Long Zhang, Yi-Xing Gao, Gang Xu, Bing-Da Sun, Wenting Liao, Yu-Qi Gao
Hypoxic preconditioning (HPC) is well‑known to exert a protective effect against hypoxic injury; however, the underlying molecular mechanism remains unclear. The present study utilized a serum metabolomics approach to detect the alterations associated with HPC. In the present study, an animal model of HPC was established by exposing adult BALB/c mice to acute repetitive hypoxia four times. The serum samples were collected by orbital blood sampling. Metabolite profiling was performed using ultra‑performance liquid chromatography‑quadrupole time‑of‑flight mass spectrometry (UPLC‑QTOFMS), in conjunction with univariate and multivariate statistical analyses...
September 13, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28900168/identification-of-key-metabolic-changes-during-liver-fibrosis-progression-in-rats-using-a-urine-and-serum-metabolomics-approach
#7
Hong Chang, Hong-Yu Meng, Shu-Min Liu, Yu Wang, Xiao-Xu Yang, Fang Lu, Hong-Yu Wang
Reversibility of hepatic fibrosis is an intrinsic response to chronic injury, and with on-going damage, fibrosis can progress to its end-stage consequence, cirrhosis. Non-invasive and reliable biomarkers for early detection of liver fibrosis are needed. Based on the CCl4-induced liver fibrosis rat model, urinary and serum metabolic profiling performed by LC-QTOF-MS associated with histological progression were utilized to identify liver fibrosis-specific potential biomarkers for early prediction and to reveal significant fibrotic pathways and their dynamic changes in different stages of liver fibrosis...
September 12, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28900150/high-throughput-data-analyses-of-the-immune-characteristics-of-microtus-fortis-infected-with-schistosoma-japonicum
#8
Yuan Hu, Lei Sun, Zhongying Yuan, Yuxin Xu, Jianping Cao
Microtus fortis exhibits natural resistance against Schistosoma japonicum, and the parasite cannot grow and develop in M. fortis. Extensive research has been carried out, however, the associated mechanism remains unclear. In the present study, we analysed the combined data obtained from a cytokine chip assay, transcriptome, and metabolome. The cytokine profile from C57BL/6 and M. fortis mice was assessed before and after infection. Several cytokines increased during the second and third week post-infection...
September 12, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28883404/identification-of-2-hydroxyisocaproic-acid-production-in-lactic-acid-bacteria-and-evaluation-of-microbial-dynamics-during-kimchi-ripening
#9
Boyeon Park, Hyelyeon Hwang, Ji Yoon Chang, Sung Wook Hong, Se Hee Lee, Min Young Jung, Sung-Oh Sohn, Hae Woong Park, Jong-Hee Lee
Lactic acid bacteria produce diverse functional metabolites in fermented foods. However, little is known regarding the metabolites and the fermentation process in kimchi. In this study, the culture broth from Leuconostoc lactis, a lactic acid bacterium isolated from kimchi, was analysed by liquid chromatography-tandem mass spectrometry and identified by the MS-DIAL program. The MassBank database was used to analyse the metabolites produced during fermentation. A mass spectrum corresponding to 2-hydroxyisocaproic acid (HICA) was validated based on a collision-induced dissociation (CID) fragmentation pattern with an identified m/z value of 131...
September 7, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28878744/guanidine-n-methylation-by-blsl-is-dependent-on-acylation-of-beta-amine-arginine-in-the-biosynthesis-of-blasticidin-s
#10
Xiankun Wang, Aiqin Du, Guiyang Yu, Zixin Deng, Xinyi He
The peptidyl nucleoside blasticidin S (BS) produced by Streptomyces griseochromogenes was the first non-mercurial fungicide used to prevent rice blast and increasingly used as a selection reagent in transgenic study. Acylation by addition of a leucine residue at the beta amine group of arginine side chain of demethylblasticidin S (DBS) has been proposed as a novel self-resistance to the cytotoxic biosynthetic intermediate. But the resultant product leucyldemethylblasticidin S (LDBS) has not been isolated as a metabolite, and LDBS synthetase activity remained to be demonstrated in S...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28874840/uplc-q-tof-ms-based-metabonomic-studies-on-the-intervention-effects-of-aspirin-eugenol-ester-in-atherosclerosis-hamsters
#11
Ning Ma, Yajun Yang, Xiwang Liu, Xiaojun Kong, Shihong Li, Zhe Qin, Zenghua Jiao, Jianyong Li
Based on the pro-drug principle, aspirin and eugenol were used to synthesize aspirin eugenol ester (AEE) by esterification reaction. In present study, the anti-atherosclerosis effects of AEE were investigated in hamsters with the utilization of metabonomic approach based on UPLC-Q-TOF/MS. Biochemical parameters and histopathological injures in stomach, liver and aorta were evaluated. In atherosclerotic hamster, oral administration of AEE normalized biochemical profile such as reducing TG, TCH and LDL, and significantly reduced body weight gain, alleviated hepatic steatosis and improved pathological lesions in aorta...
September 5, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28857222/ostgap1-is-responsible-for-ja-inducible-diterpenoid-phytoalexin-biosynthesis-in-rice-roots-with-biological-impacts-on-allelopathic-interaction
#12
Yuri Yoshida, Koji Miyamoto, Hisakazu Yamane, Yoko Nishizawa, Eiichi Minami, Hideaki Nojiri, Kazunori Okada
Phytocassanes and momilactones are known as major diterpenoid phytoalexins (DPs), characterized by abundant production and antimicrobial activity and their biosynthetic genes are clustered in rice genomes. The basic leucine zipper transcription factor OsTGAP1 is known to act as a regulator of the coordinated production of DPs in cultured rice cells, but the in planta functions of OsTGAP1 remain largely unknown. Here, we present evidence on the biological function of OsTGAP1 in planta. In wild-type plants, OsTGAP1 is abundantly expressed in roots compared to that in shoots...
August 30, 2017: Physiologia Plantarum
https://www.readbyqxmd.com/read/28847019/metabolomic-guided-isolation-of-bioactive-natural-products-from-curvularia-sp-an-endophytic-fungus-of-terminalia-laxiflora
#13
Ahmed F Tawfike, Grainne Abbott, Louise Young, RuAngelie Edrada-Ebel
Endophytic fungi associated with medicinal plants are a potential source of novel chemistry and biology. Metabolomic tools were successfully employed to compare the metabolite fingerprints of solid and liquid culture extracts of endophyte Curvularia sp. isolated from the leaves of Terminalia laxiflora. Natural product databases were used to dereplicate metabolites in order to determine known compounds and the presence of new natural products. Multivariate analysis highlighted the putative metabolites responsible for the bioactivity of the fungal extract and its fractions on NF-κB and the myelogenous leukemia cell line K562...
August 28, 2017: Planta Medica
https://www.readbyqxmd.com/read/28841695/earwax-metabolomics-an-innovative-pilot-metabolic-profiling-study-for-assessing-metabolic-changes-in-ewes-during-periparturition-period
#14
Engy Shokry, Julião Pereira, Jair Gonzalez Marques Júnior, Paulo Henrique Jorge da Cunha, Antônio Dionísio Feitosa Noronha Filho, Jessica Alves da Silva, Maria Clorinda Soares Fioravanti, Anselmo Elcana de Oliveira, Nelson Roberto Antoniosi Filho
Important metabolic changes occur during transition period of late pregnancy and early lactation to meet increasing energy demands of the growing fetus and for milk production. The aim of this investigation is to present an innovative and non-invasive tool using ewe earwax sample analysis to assess the metabolic profile in ewes during late pregnancy and early lactation. In this work, earwax samples were collected from 28 healthy Brazilian Santa Inês ewes divided into 3 sub-groups: 9 non-pregnant ewes, 6 pregnant ewes in the last 30 days of gestation, and 13 lactating ewes ≤ 30 days postpartum...
2017: PloS One
https://www.readbyqxmd.com/read/28840775/untargeted-metabolomic-analysis-and-pathway-discovery-in-perinatal-asphyxia-and-hypoxic-ischaemic-encephalopathy
#15
Niamh M Denihan, Jennifer A Kirwan, Brian H Walsh, Warwick B Dunn, David I Broadhurst, Geraldine B Boylan, Deirdre M Murray
Elucidating metabolic effects of hypoxic-ischaemic encephalopathy (HIE) may reveal early biomarkers of injury and new treatment targets. This study uses untargeted metabolomics to examine early metabolic alterations in a carefully defined neonatal population. Infants with perinatal asphyxia who were resuscitated at birth and recovered (PA group), those who developed HIE (HIE group) and healthy controls were all recruited at birth. Metabolomic analysis of cord blood was performed using direct infusion FT-ICR mass spectrometry...
January 1, 2017: Journal of Cerebral Blood Flow and Metabolism
https://www.readbyqxmd.com/read/28836978/diurnal-variation-of-nmr-based-blood-metabolites-in-calves-fed-a-high-plane-of-milk-replacer-a-pilot-study
#16
Morteza H Ghaffari, Jayden A R MacPherson, Harma Berends, Michael A Steele
BACKGROUND: Blood profiles have been used to monitor herd health status, diagnose disorders, and predict the risk of diseases in cattle and calves. Characterizing plasma metabolites in dairy calves could provide further insight into daily metabolic variations and the mechanisms that lead to metabolic diseases. In addition, by understanding physiological ranges of plasma metabolites relative to meal and the time of feeding in healthy animals, veterinarians can accurately diagnose abnormalities with a blood test...
August 23, 2017: BMC Veterinary Research
https://www.readbyqxmd.com/read/28826373/fatty-acids-and-nlrp3-inflammasome-mediated-inflammation-in-metabolic-tissues
#17
Jessica C Ralston, Claire L Lyons, Elaine B Kennedy, Anna M Kirwan, Helen M Roche
Worldwide obesity rates have reached epidemic proportions and significantly contribute to the growing prevalence of metabolic diseases. Chronic low-grade inflammation, a hallmark of obesity, involves immune cell infiltration into expanding adipose tissue. In turn, obesity-associated inflammation can lead to complications in other metabolic tissues (e.g., liver, skeletal muscle, pancreas) through lipotoxicity and inflammatory signaling networks. Importantly, although numerous signaling pathways are known to integrate metabolic and inflammatory processes, the nucleotide-binding and oligomerization domain-like receptor, leucine-rich repeat and pyrin domain-containing 3 (NLRP3) inflammasome is now noted to be a key regulator of metabolic inflammation...
August 21, 2017: Annual Review of Nutrition
https://www.readbyqxmd.com/read/28811139/metabonomics-approach-to-assessing-the-metabolism-variation-and-gender-gap-of-drosophila-melanogaster-in-aging-process
#18
Yu-Zhi Zhou, Ming-Liang Yan, Li Gao, Jian-Qin Zhang, Xue-Mei Qin, Xiang Zhang, Guan-Hua Du
Drosophila melanogaster is increasingly used for study aging mechanism and evaluating anti-aging drugs, but the changes of metabolites and differences of metabolites change between male and female during the aging process are not well known. Metabolomics technology, a massive information provider, has promoted the understanding of metabolic profile and overall changes of metabolites in organism. In this study, (1)H NMR based metabonomics was employed to investigate the dynamic changes of metabolites in whole bodies of male and female Drosophila melanogaster at 3, 15, 30, 45days and to research the gender gap of metabolites changes in aging process...
August 12, 2017: Experimental Gerontology
https://www.readbyqxmd.com/read/28806601/influence-of-sublethal-doses-of-acetamiprid-and-halosulfuron-methyl-on-metabolites-of-zebra-fish-brachydanio-rerio
#19
Hong Zhang, Lijuan Zhao
Acetamiprid, a neonicotinoid pesticide, is reported to have adverse sublethal effects on non-target beneficial organisms. Halosulfuron-methyl (HM), one of the most widely used herbicides in agriculture, has high ecotoxicity to aquatic plants and animals. In this study, a GC-MS-based metabolomics approach was used to investigate the toxicity of acetamiprid and HM. The Automated Mass Spectral Deconvolution and Identification System (AMDIS) software program and the retention index method were used to identify 51 metabolites in zebra fish (Brachydanio rerio)...
August 4, 2017: Aquatic Toxicology
https://www.readbyqxmd.com/read/28796264/lytic-cell-death-induced-by-melittin-bypasses-pyroptosis-but-induces-nlrp3-inflammasome-activation-and-il-1%C3%AE-release
#20
Fátima Martín-Sánchez, Juan José Martínez-García, María Muñoz-García, Miriam Martínez-Villanueva, José A Noguera-Velasco, David Andreu, Luís Rivas, Pablo Pelegrín
The nucleotide-binding domain and leucine-rich repeat-containing receptor with a pyrin domain 3 (NLRP3) inflammasome is a sensor for different types of infections and alterations of homeostatic parameters, including abnormally high levels of the extracellular nucleotide ATP or crystallization of different metabolites. All NLRP3 activators trigger a similar intracellular pathway, where a decrease in intracellular K(+) concentration and permeabilization of plasma membrane are key steps. Cationic amphipathic antimicrobial peptides and peptide toxins permeabilize the plasma membrane...
August 10, 2017: Cell Death & Disease
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