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https://www.readbyqxmd.com/read/28338918/integrated-mirna-and-mrna-expression-profiling-reveals-the-response-regulators-of-a-susceptible-tomato-cultivar-to-early-blight-disease
#1
Deepti Sarkar, Ranjan Kumar Maji, Sayani Dey, Arijita Sarkar, Zhumur Ghosh, Pallob Kundu
Early blight, caused by the fungus Alternaria solani, is a devastating foliar disease of tomatoes, causes massive yield loss each year worldwide. Molecular basis of the compatible host-pathogen interaction was elusive. We adopted next generation sequencing approach to decipher miRNAs and mRNAs that are differentially expressed during Alternaria-stress in tomato. Some of the interesting findings were also validated by alternative techniques. Our analysis revealed 181 known-miRNAs, belonging to 121 miRNA families, of which 67 miRNAs showed at least 2-fold change in expression level with the majority being downregulated...
March 3, 2017: DNA Research: An International Journal for Rapid Publication of Reports on Genes and Genomes
https://www.readbyqxmd.com/read/28338798/genetic-dissection-of-metabolite-variation-in-arabidopsis-seeds-evidence-for-mqtl-hotspots-and-a-master-regulatory-locus-of-seed-metabolism
#2
Dominic Knoch, David Riewe, Rhonda Christiane Meyer, Anastassia Boudichevskaia, Renate Schmidt, Thomas Altmann
To gain insight into genetic factors controlling seed metabolic composition and its relationship to major seed properties, an Arabidopsis recombinant inbred line (RIL) population, derived from accessions Col-0 and C24, was studied using an MS-based metabolic profiling approach. Relative intensities of 311 polar primary metabolites were used to identify associated genomic loci and to elucidate their interactions by quantitative trait locus (QTL) mapping. A total of 786 metabolic QTLs (mQTLs) were unequally distributed across the genome, forming several hotspots...
March 6, 2017: Journal of Experimental Botany
https://www.readbyqxmd.com/read/28299975/targeted-high-performance-liquid-chromatography-tandem-mass-spectrometry-based-metabolomics-differentiates-metabolic-syndrome-from-obesity
#3
Fanyi Zhong, Mengyang Xu, Richard S Bruno, Kevin D Ballard, Jiangjiang Zhu
Both obesity and the metabolic syndrome are risk factors for type 2 diabetes and cardiovascular disease. Identification of novel biomarkers are needed to distinguish metabolic syndrome from equally obese individuals in order to direct them to early interventions that reduce their risk of developing further health problems. We utilized mass spectrometry-based targeted metabolic profiling of 221 metabolites to evaluate the associations between metabolite profiles and established metabolic syndrome criteria (i...
April 2017: Experimental Biology and Medicine
https://www.readbyqxmd.com/read/28292328/age-dependent-dopamine-transporter-dysfunction-and-serine129-phospho-%C3%AE-synuclein-overload-in-g2019s-lrrk2-mice
#4
Francesco Longo, Daniela Mercatelli, Salvatore Novello, Ludovico Arcuri, Alberto Brugnoli, Fabrizio Vincenzi, Isabella Russo, Giulia Berti, Omar S Mabrouk, Robert T Kennedy, Derya R Shimshek, Katia Varani, Luigi Bubacco, Elisa Greggio, Michele Morari
Mutations in the leucine-rich repeat kinase 2 (LRRK2) gene are the most common genetic cause of Parkinson's disease. Here, we investigated whether the G2019S LRRK2 mutation causes morphological and/or functional changes at nigro-striatal dopamine neurons. Density of striatal dopaminergic terminals, nigral cell counts, tyrosine hydroxylase protein levels as well as exocytotic dopamine release measured in striatal synaptosomes, or striatal extracellular dopamine levels monitored by in vivo microdialysis were similar between ≥12-month-old G2019S knock-in mice and wild-type controls...
March 14, 2017: Acta Neuropathologica Communications
https://www.readbyqxmd.com/read/28291661/evaluation-of-a-novel-system-for-analyzing-hydrophilic-blood-metabolites
#5
Shin Nishiumi, Keisuke Shima, Takeshi Azuma, Masaru Yoshida
Metabolomics has recently been developed, and there have been a considerable number of metabolomics-based biomarker studies in the medical research field. Therefore, as a first step toward the practical use of metabolite biomarkers, a simple and quick sample preparation method involving metabolite extraction, metabolite measurement, and data analysis needs to be developed. In this study, we evaluated whether the use of simpler metabolite extraction methods would facilitate the stable analysis of hydrophilic blood metabolites during liquid chromatography/triple quadrupole mass spectrometry (LC/QqQMS)-based metabolome analysis...
March 10, 2017: Journal of Bioscience and Bioengineering
https://www.readbyqxmd.com/read/28266220/metabolic-mechanism-for-l-leucine-induced-metabolome-to-eliminate-streptococcus-iniae
#6
Chao-Chao Du, Manjun Yang, Min-Yi Li, Jun Yang, Bo Peng, Hui Li, Xuan-Xian Peng
Crucial metabolites that modulate hosts' metabolome to eliminate bacterial pathogens have been documented, but the metabolic mechanisms are largely unknown. The present study explores the metabolic mechanism for L-leucine-induced metabolome to eliminate Streptococcus iniae in tilapia. GC-MS based metabolomics was used to investigate tilapia liver metabolic profile in the presence of exogenous L-leucine. Thirty-seven metabolites of differential abundance were determined, and eleven metabolic pathways were enriched...
March 7, 2017: Journal of Proteome Research
https://www.readbyqxmd.com/read/28259749/metabolic-rewiring-in-cancer-cells-overexpressing-the-glucocorticoid-induced-leucine-zipper-protein-gilz-activation-of-mitochondrial-oxidative-phosphorylation-and-sensitization-to-oxidative-cell-death-induced-by-mitochondrial-targeted-drugs
#7
Fanny André, Anne Trinh, Stéphane Balayssac, Patrice Maboudou, Salim Dekiouk, Myriam Malet-Martino, Bruno Quesnel, Thierry Idziorek, Jérome Kluza, Philippe Marchetti
Cancer cell metabolism is largely controlled by oncogenic signals and nutrient availability. Here, we highlighted that the glucocorticoid-induced leucine zipper (GILZ), an intracellular protein influencing many signaling pathways, reprograms cancer cell metabolism to promote proliferation. We provided evidence that GILZ overexpression induced a significant increase of mitochondrial oxidative phosphorylation as evidenced by the augmentation in basal respiration, ATP-linked respiration as well as respiratory capacity...
March 1, 2017: International Journal of Biochemistry & Cell Biology
https://www.readbyqxmd.com/read/28252223/chronic-effects-of-copper-in-oysters-crassostrea-hongkongensis-under-different-exposure-regimes-as-shown-by-nmr-based-metabolomics
#8
Chen Cao, Wen-Xiong Wang
Traditional metal toxicity tests on organisms have mainly focused on continuous exposure at a fixed concentration. However, organisms are more likely exposed to pollutants intermittently in estuarine environments that are significantly impacted by anthropogenic activity. The present study examined whether different copper (Cu) exposure regimes at an equivalent dose can induce different metabolomics effects on the oysters. An estuarine oyster Crassostrea hongkongensis was exposed to Cu continuously or intermittently at an equal dose (time × concentration) for 6 wk...
March 2, 2017: Environmental Toxicology and Chemistry
https://www.readbyqxmd.com/read/28237996/macromolecular-and-elemental-composition-analyses-of-leuconostoc-mesenteroides-atcc-8293-cultured-in-a-chemostat
#9
Jeongsu Bang, Ling Li, Hyunbin Seong, Ye Won Kwon, Dong-Yup Lee, Nam Soo Han
The cellular composition and metabolic compounds of Leuconostoc mesenteroides ATCC 8293 were analyzed after cultivation in an anaerobic chemostat. Macromolecular composition was 24.4% polysaccharide, 29.7% protein, 7.9% lipid, 2.9% DNA, and 7.4% RNA. Amino acid compositions included large amounts of lysine, glutamic acid, alanine, and leucine. Elements were in order of C > O > N > H > S. The metabolites in chemostat were lactic acid (73.34 mM), acetic acid (7.69 mM), and mannitol (9.93 mM). These data provide a first view of the cellular composition of L...
February 24, 2017: Journal of Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28223686/metabolic-profiling-of-presymptomatic-huntington-s-disease-sheep-reveals-novel-biomarkers
#10
Debra J Skene, Benita Middleton, Cara K Fraser, Jeroen L A Pennings, Timothy R Kuchel, Skye R Rudiger, C Simon Bawden, A Jennifer Morton
The pronounced cachexia (unexplained wasting) seen in Huntington's disease (HD) patients suggests that metabolic dysregulation plays a role in HD pathogenesis, although evidence of metabolic abnormalities in HD patients is inconsistent. We performed metabolic profiling of plasma from presymptomatic HD transgenic and control sheep. Metabolites were quantified in sequential plasma samples taken over a 25 h period using a targeted LC/MS metabolomics approach. Significant changes with respect to genotype were observed in 89/130 identified metabolites, including sphingolipids, biogenic amines, amino acids and urea...
February 22, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28209137/transcriptional-analysis-of-defense-mechanisms-in-upland-tetraploid-switchgrass-to-greenbugs
#11
Teresa Donze-Reiner, Nathan A Palmer, Erin D Scully, Travis J Prochaska, Kyle G Koch, Tiffany Heng-Moss, Jeffrey D Bradshaw, Paul Twigg, Keenan Amundsen, Scott E Sattler, Gautam Sarath
BACKGROUND: Aphid infestation of switchgrass (Panicum virgatum) has the potential to reduce yields and biomass quality. Although switchgrass-greenbug (Schizaphis graminum; GB) interactions have been studied at the whole plant level, little information is available on plant defense responses at the molecular level. RESULTS: The global transcriptomic response of switchgrass cv Summer to GB was monitored by RNA-Seq in infested and control (uninfested) plants harvested at 5, 10, and 15 days after infestation (DAI)...
February 16, 2017: BMC Plant Biology
https://www.readbyqxmd.com/read/28203242/sunlight-modulates-fruit-metabolic-profile-and-shapes-the-spatial-pattern-of-compound-accumulation-within-the-grape-cluster
#12
Noam Reshef, Natasha Walbaum, Nurit Agam, Aaron Fait
Vineyards are characterized by their large spatial variability of solar irradiance (SI) and temperature, known to effectively modulate grape metabolism. To explore the role of sunlight in shaping fruit composition and cluster uniformity, we studied the spatial pattern of incoming irradiance, fruit temperature and metabolic profile within individual grape clusters under three levels of sunlight exposure. The experiment was conducted in a vineyard of Cabernet Sauvignon cv. located in the Negev Highlands, Israel, where excess SI and midday temperatures are known to degrade grape quality...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28194987/mass-defect-based-n-n-dimethyl-leucine-labels-for-quantitative-proteomics-and-amine-metabolomics-of-pancreatic-cancer-cells
#13
Ling Hao, Jillian Johnson, Christopher B Lietz, Amanda Buchberger, Dustin Frost, W John Kao, Lingjun Li
Mass spectrometry-based stable isotope labeling has become a key technology for protein and small-molecule analyses. We developed a multiplexed quantification method for simultaneous proteomics and amine metabolomics analyses via nano reversed-phase liquid chromatography-tandem mass spectrometry (nanoRPLC-MS/MS), called mass defect-based N,N-dimethyl leucine (mdDiLeu) labeling. The duplex mdDiLeu reagents were custom-synthesized with a mass difference of 20.5 mDa, arising from the subtle variation in nuclear binding energy between the two DiLeu isotopologues...
January 17, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28189056/metabolic-responses-to-intestine-regeneration-in-sea-cucumbers-apostichopus-japonicus
#14
Lina Sun, Jingchun Sun, Qinzeng Xu, Xiaoni Li, Libin Zhang, Hongsheng Yang
Sea cucumbers are excellent models for studying organ regeneration due to their striking capacity to regenerate most of their viscera after evisceration. In this study, we applied NMR-based metabolomics to determine the metabolite changes that occur during the process of intestine regeneration in sea cucumbers. Partial least-squares discriminant analysis showed that there was significant differences in metabolism between regenerative intestines at 3, 7, and 14days post evisceration (dpe) and normal intestines...
February 4, 2017: Comparative Biochemistry and Physiology. Part D, Genomics & Proteomics
https://www.readbyqxmd.com/read/28185532/the-metabolites-in-peripheral-blood-mononuclear-cells-showed-greater-differences-between-patients-with-impaired-fasting-glucose-or-type-2-diabetes-and-healthy-controls-than-those-in-plasma
#15
Minjoo Kim, Minkyung Kim, Ji Yun Han, Sang-Hyun Lee, Sun Ha Jee, Jong Ho Lee
To determine differences between peripheral blood mononuclear cells and the plasma metabolites in patients with impaired fasting glucose or type 2 diabetes and healthy controls. In all, 65 nononobese patients (aged 30-70 years) with impaired fasting glucose or type 2 diabetes and 65 nonobese sex-matched healthy controls were included, and fasting peripheral blood mononuclear cell and plasma metabolomes were profiled. The diabetic or impaired fasting glucose patients showed higher circulating and peripheral blood mononuclear cell lipoprotein phospholipase A2 activities, high-sensitivity C-reactive protein and tumour necrosis factor-α than controls...
March 2017: Diabetes & Vascular Disease Research
https://www.readbyqxmd.com/read/28178975/evaluation-of-dogs-with-genetic-hyperuricosuria-and-urate-urolithiasis-consuming-a-purine-restricted-diet-a-pilot-study
#16
Jodi L Westropp, Jennifer A Larsen, Eric G Johnson, Dannika Bannasch, Andrea J Fascetti, Vincent Biourge, Yann Queau
BACKGROUND: Urate urolithiasis is a common problem in breed homozygous for the mutation that results in hyperuricosuria. Low purine diets have been recommended to reduce purine intake in these dogs. METHODS: A higher protein, purine restricted diet with water added was evaluated in dogs with genetic hyperuricosuria and a history of clinical urate urolithiasis over a one year time period. Dogs were evaluated at baseline and 2, 6, and 12 months after initiating the test diet...
February 8, 2017: BMC Veterinary Research
https://www.readbyqxmd.com/read/28145477/efficient-electrochemical-remediation-of-microcystin-lr-in-tap-water-using-designer-tio2-carbon-electrodes
#17
Germán Sanz Lobón, Alfonso Yepez, Luane Ferreira Garcia, Ruiter Lima Morais, Boniek Gontijo Vaz, Veronica Vale Carvalho, Gisele Augusto Rodrigues de Oliveira, Rafael Luque, Eric de Souza Gil
Microcystin-leucine arginine (MC-LR) is the most abundant and toxic secondary metabolite produced by freshwater cyanobacteria. This toxin has a high potential hazard health due to potential interactions with liver, kidney and the nervous system. The aim of this work was the design of a simple and environmentally friendly electrochemical system based on highly efficient nanostructured electrodes for the removal of MC-LR in tap water. Titania nanoparticles were deposited on carbon (graphite) under a simple and efficient microwave assisted approach for the design of the electrode, further utilized in the electrochemical remediation assays...
February 1, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28132861/global-and-untargeted-metabolomics-evidence-of-the-protective-effect-of-different-extracts-of-dipsacus-asper-wall-ex-c-b-clarke-on-estrogen-deficiency-after-ovariectomia-in-rats
#18
Yi Tao, Xi Chen, Weidong Li, Baochang Cai, Liuqing Di, Liyun Shi, Lihong Hu
ETHNOPHARMACOLOGICAL RELEVANCE: Dipsacus asper Wall. ex C.B. Clarke (DA) is an effectively traditional Chinese medicine for treating osteoporosis and bone fracture. Until now, studies on pharmacological mechanism of DA mostly centered on cell and gene level, little is known about its metabolic signatures. The aim of this study is to investigate the anti-osteoporosis effects of crude and wine-processed DA by global and untargeted metabolic profiling of rats serum, liver and kidney derived from sham, model and treated groups...
January 26, 2017: Journal of Ethnopharmacology
https://www.readbyqxmd.com/read/28129369/serum-metabolomics-of-burkitt-lymphoma-mouse-models
#19
Fengmin Yang, Jie Du, Hong Zhang, Guorui Ruan, Junfeng Xiang, Lixia Wang, Hongxia Sun, Aijiao Guan, Gang Shen, Yan Liu, Xiaomeng Guo, Qian Li, Yalin Tang
Burkitt lymphoma (BL) is a rare and highly aggressive type of non-Hodgkin lymphoma. The mortality rate of BL patients is very high due to the rapid growth rate and frequent systemic spread of the disease. A better understanding of the pathogenesis, more sensitive diagnostic tools and effective treatment methods for BL are essential. Metabolomics, an important aspect of systems biology, allows the comprehensive analysis of global, dynamic and endogenous biological metabolites based on their nuclear magnetic resonance (NMR) and mass spectrometry (MS)...
2017: PloS One
https://www.readbyqxmd.com/read/28111353/gc-ms-based-metabolomics-identifies-an-amino-acid-signature-of-acute-ischemic-stroke
#20
Dian Wang, Jing Kong, Jiayan Wu, Xingxing Wang, Minchao Lai
Ischemic stroke is a serious public health problem worldwide. Here, we characterized the metabolite features of acute ischemic stroke (AIS) using a gas chromatography-mass spectrometry-based metabolomics. Forty AIS patients and 29 sex and age-matched controls were recruited. The serum metabolic profiles were significantly different between the two groups. Compared to the controls, the patients had elevated levels of lactate, carbonate and glutamate (P<0.01), and lowered levels of alanine, citrate, glycine, isoleucine, leucine, serine, tyrosine, methionine, tryptophan, erythronic acid, urea, purine, hypoxanthine, and proline in the serum (P<0...
March 6, 2017: Neuroscience Letters
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