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https://www.readbyqxmd.com/read/28223686/metabolic-profiling-of-presymptomatic-huntington-s-disease-sheep-reveals-novel-biomarkers
#1
Debra J Skene, Benita Middleton, Cara K Fraser, Jeroen L A Pennings, Timothy R Kuchel, Skye R Rudiger, C Simon Bawden, A Jennifer Morton
The pronounced cachexia (unexplained wasting) seen in Huntington's disease (HD) patients suggests that metabolic dysregulation plays a role in HD pathogenesis, although evidence of metabolic abnormalities in HD patients is inconsistent. We performed metabolic profiling of plasma from presymptomatic HD transgenic and control sheep. Metabolites were quantified in sequential plasma samples taken over a 25 h period using a targeted LC/MS metabolomics approach. Significant changes with respect to genotype were observed in 89/130 identified metabolites, including sphingolipids, biogenic amines, amino acids and urea...
February 22, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28209137/transcriptional-analysis-of-defense-mechanisms-in-upland-tetraploid-switchgrass-to-greenbugs
#2
Teresa Donze-Reiner, Nathan A Palmer, Erin D Scully, Travis J Prochaska, Kyle G Koch, Tiffany Heng-Moss, Jeffrey D Bradshaw, Paul Twigg, Keenan Amundsen, Scott E Sattler, Gautam Sarath
BACKGROUND: Aphid infestation of switchgrass (Panicum virgatum) has the potential to reduce yields and biomass quality. Although switchgrass-greenbug (Schizaphis graminum; GB) interactions have been studied at the whole plant level, little information is available on plant defense responses at the molecular level. RESULTS: The global transcriptomic response of switchgrass cv Summer to GB was monitored by RNA-Seq in infested and control (uninfested) plants harvested at 5, 10, and 15 days after infestation (DAI)...
February 16, 2017: BMC Plant Biology
https://www.readbyqxmd.com/read/28203242/sunlight-modulates-fruit-metabolic-profile-and-shapes-the-spatial-pattern-of-compound-accumulation-within-the-grape-cluster
#3
Noam Reshef, Natasha Walbaum, Nurit Agam, Aaron Fait
Vineyards are characterized by their large spatial variability of solar irradiance (SI) and temperature, known to effectively modulate grape metabolism. To explore the role of sunlight in shaping fruit composition and cluster uniformity, we studied the spatial pattern of incoming irradiance, fruit temperature and metabolic profile within individual grape clusters under three levels of sunlight exposure. The experiment was conducted in a vineyard of Cabernet Sauvignon cv. located in the Negev Highlands, Israel, where excess SI and midday temperatures are known to degrade grape quality...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28194987/mass-defect-based-n-n-dimethyl-leucine-labels-for-quantitative-proteomics-and-amine-metabolomics-of-pancreatic-cancer-cells
#4
Ling Hao, Jillian Johnson, Christopher B Lietz, Amanda Buchberger, Dustin Frost, W John Kao, Lingjun Li
Mass spectrometry-based stable isotope labeling has become a key technology for protein and small-molecule analyses. We developed a multiplexed quantification method for simultaneous proteomics and amine metabolomics analyses via nano reversed-phase liquid chromatography-tandem mass spectrometry (nanoRPLC-MS/MS), called mass defect-based N,N-dimethyl leucine (mdDiLeu) labeling. The duplex mdDiLeu reagents were custom-synthesized with a mass difference of 20.5 mDa, arising from the subtle variation in nuclear binding energy between the two DiLeu isotopologues...
January 17, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28189056/metabolic-responses-to-intestine-regeneration-in-sea-cucumbers-apostichopus-japonicus
#5
Lina Sun, Jingchun Sun, Qinzeng Xu, Xiaoni Li, Libin Zhang, Hongsheng Yang
Sea cucumbers are excellent models for studying organ regeneration due to their striking capacity to regenerate most of their viscera after evisceration. In this study, we applied NMR-based metabolomics to determine the metabolite changes that occur during the process of intestine regeneration in sea cucumbers. Partial least-squares discriminant analysis showed that there was significant differences in metabolism between regenerative intestines at 3, 7, and 14days post evisceration (dpe) and normal intestines...
February 4, 2017: Comparative Biochemistry and Physiology. Part D, Genomics & Proteomics
https://www.readbyqxmd.com/read/28185532/the-metabolites-in-peripheral-blood-mononuclear-cells-showed-greater-differences-between-patients-with-impaired-fasting-glucose-or-type-2-diabetes-and-healthy-controls-than-those-in-plasma
#6
Minjoo Kim, Minkyung Kim, Ji Yun Han, Sang-Hyun Lee, Sun Ha Jee, Jong Ho Lee
To determine differences between peripheral blood mononuclear cells and the plasma metabolites in patients with impaired fasting glucose or type 2 diabetes and healthy controls. In all, 65 nononobese patients (aged 30-70 years) with impaired fasting glucose or type 2 diabetes and 65 nonobese sex-matched healthy controls were included, and fasting peripheral blood mononuclear cell and plasma metabolomes were profiled. The diabetic or impaired fasting glucose patients showed higher circulating and peripheral blood mononuclear cell lipoprotein phospholipase A2 activities, high-sensitivity C-reactive protein and tumour necrosis factor-α than controls...
March 2017: Diabetes & Vascular Disease Research
https://www.readbyqxmd.com/read/28178975/evaluation-of-dogs-with-genetic-hyperuricosuria-and-urate-urolithiasis-consuming-a-purine-restricted-diet-a-pilot-study
#7
Jodi L Westropp, Jennifer A Larsen, Eric G Johnson, Dannika Bannasch, Andrea J Fascetti, Vincent Biourge, Yann Queau
BACKGROUND: Urate urolithiasis is a common problem in breed homozygous for the mutation that results in hyperuricosuria. Low purine diets have been recommended to reduce purine intake in these dogs. METHODS: A higher protein, purine restricted diet with water added was evaluated in dogs with genetic hyperuricosuria and a history of clinical urate urolithiasis over a one year time period. Dogs were evaluated at baseline and 2, 6, and 12 months after initiating the test diet...
February 8, 2017: BMC Veterinary Research
https://www.readbyqxmd.com/read/28145477/efficient-electrochemical-remediation-of-microcystin-lr-in-tap-water-using-designer-tio2-carbon-electrodes
#8
Germán Sanz Lobón, Alfonso Yepez, Luane Ferreira Garcia, Ruiter Lima Morais, Boniek Gontijo Vaz, Veronica Vale Carvalho, Gisele Augusto Rodrigues de Oliveira, Rafael Luque, Eric de Souza Gil
Microcystin-leucine arginine (MC-LR) is the most abundant and toxic secondary metabolite produced by freshwater cyanobacteria. This toxin has a high potential hazard health due to potential interactions with liver, kidney and the nervous system. The aim of this work was the design of a simple and environmentally friendly electrochemical system based on highly efficient nanostructured electrodes for the removal of MC-LR in tap water. Titania nanoparticles were deposited on carbon (graphite) under a simple and efficient microwave assisted approach for the design of the electrode, further utilized in the electrochemical remediation assays...
February 1, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28132861/global-and-untargeted-metabolomics-evidence-of-the-protective-effect-of-different-extracts-of-dipsacus-asper-wall-ex-c-b-clarke-on-estrogen-deficiency-after-ovariectomia-in-rats
#9
Yi Tao, Xi Chen, Weidong Li, Baochang Cai, Liuqing Di, Liyun Shi, Lihong Hu
ETHNOPHARMACOLOGICAL RELEVANCE: Dipsacus asper Wall. ex C.B. Clarke (DA) is an effectively traditional Chinese medicine for treating osteoporosis and bone fracture. Until now, studies on pharmacological mechanism of DA mostly centered on cell and gene level, little is known about its metabolic signatures. The aim of this study is to investigate the anti-osteoporosis effects of crude and wine-processed DA by global and untargeted metabolic profiling of rats serum, liver and kidney derived from sham, model and treated groups...
January 26, 2017: Journal of Ethnopharmacology
https://www.readbyqxmd.com/read/28129369/serum-metabolomics-of-burkitt-lymphoma-mouse-models
#10
Fengmin Yang, Jie Du, Hong Zhang, Guorui Ruan, Junfeng Xiang, Lixia Wang, Hongxia Sun, Aijiao Guan, Gang Shen, Yan Liu, Xiaomeng Guo, Qian Li, Yalin Tang
Burkitt lymphoma (BL) is a rare and highly aggressive type of non-Hodgkin lymphoma. The mortality rate of BL patients is very high due to the rapid growth rate and frequent systemic spread of the disease. A better understanding of the pathogenesis, more sensitive diagnostic tools and effective treatment methods for BL are essential. Metabolomics, an important aspect of systems biology, allows the comprehensive analysis of global, dynamic and endogenous biological metabolites based on their nuclear magnetic resonance (NMR) and mass spectrometry (MS)...
2017: PloS One
https://www.readbyqxmd.com/read/28111353/gc-ms-based-metabolomics-identifies-an-amino-acid-signature-of-acute-ischemic-stroke
#11
Dian Wang, Jing Kong, Jiayan Wu, Xingxing Wang, Minchao Lai
Ischemic stroke is a serious public health problem worldwide. Here, we characterized the metabolite features of acute ischemic stroke (AIS) using a gas chromatography-mass spectrometry-based metabolomics. Forty AIS patients and 29 sex and age-matched controls were recruited. The serum metabolic profiles were significantly different between the two groups. Compared to the controls, the patients had elevated levels of lactate, carbonate and glutamate (P<0.01), and lowered levels of alanine, citrate, glycine, isoleucine, leucine, serine, tyrosine, methionine, tryptophan, erythronic acid, urea, purine, hypoxanthine, and proline in the serum (P<0...
January 19, 2017: Neuroscience Letters
https://www.readbyqxmd.com/read/28092089/time-dependent-lactate-production-and-amino-acid-utilization-in-cultured-astrocytes-under-high-glucose-exposure
#12
Dan Wang, Liangcai Zhao, Hong Zheng, Minjian Dong, Linlin Pan, Xi Zhang, Huajie Zhang, Hongchang Gao
Accumulating investigations have focused on the severity of central nervous system (CNS) complications in diabetic patients. The effects of the high glucose (HG) probably attribute to the metabolic disturbances in CNS. Astrocytes, with powerful ability of metabolic regulation, play crucial roles in physiological and pathological processes in CNS. Hence, an in-depth analysis as to metabolic alterations of astrocytes exposure to HG would facilitate to explore the underlying pathogenesis. In this study, the (1)H NMR-based metabonomic approach was performed to characterize the metabolic variations of intracellular metabolites and corresponding culture media in a time-dependent manner...
January 16, 2017: Molecular Neurobiology
https://www.readbyqxmd.com/read/28088662/metabolic-profiles-of-exudates-from-chronic-leg-ulcerations
#13
Adam Junka, Wojciech Wojtowicz, Adam Ząbek, Grzegorz Krasowski, Danuta Smutnicka, Barbara Bakalorz, Agnieszka Boruta, Mariusz Dziadas, Piotr Młynarz, Parish Paymon Sedghizadeh, Marzenna Bartoszewicz
Chronic leg ulceration is a disease usually associated with other comorbidities, and significantly reduces patient quality of life. Infected leg ulcers can lead to limb-threatening sequelae or mortality. Leg ulcerations are colonized by a number of microbes that are able to cause life-threating infections in susceptible patients. Wound exudate is a body fluid that collects metabolites from patient eukaryotic cells and from prokaryotic bacterial communities inhabiting the wound. This study aimed at identification of metabolites in exudates collected from chronic leg ulcers, and correlation of this metabolome with patient comorbidities and microbiological status of the wound...
January 10, 2017: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/28077967/integrated-omics-analyses-reveal-the-details-of-metabolic-adaptation-of-clostridium-thermocellum-to-lignocellulose-derived-growth-inhibitors-released-during-the-deconstruction-of-switchgrass
#14
Suresh Poudel, Richard J Giannone, Miguel Rodriguez, Babu Raman, Madhavi Z Martin, Nancy L Engle, Jonathan R Mielenz, Intawat Nookaew, Steven D Brown, Timothy J Tschaplinski, David Ussery, Robert L Hettich
BACKGROUND: Clostridium thermocellum is capable of solubilizing and converting lignocellulosic biomass into ethanol. Although much of the work-to-date has centered on characterizing this microbe's growth on model cellulosic substrates, such as cellobiose, Avicel, or filter paper, it is vitally important to understand its metabolism on more complex, lignocellulosic substrates to identify relevant industrial bottlenecks that could undermine efficient biofuel production. To this end, we have examined a time course progression of C...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/28075412/the-antagonistic-effect-of-mycotoxins-deoxynivalenol-and-zearalenone-on-metabolic-profiling-in-serum-and-liver-of-mice
#15
Jian Ji, Pei Zhu, Fangchao Cui, Fuwei Pi, Yinzhi Zhang, Yun Li, Jiasheng Wang, Xiulan Sun
Metabolic profiling in liver and serum of mice was studied for the combined toxic effects of deoxynivalenol (DON) and zearalenone (ZEN), through gas chromatography mass spectrum. The spectrum of serum and liver sample of mice, treated with individual 2 mg/kg DON, 20 mg/kg ZEN, and the combined DON + ZEN with final concentration 2 mg/kg DON and 20 mg/kg ZEN for 21 days, were deconvoluted, aligned and identified with MS DIAL. The data matrix was processed with univariate analysis and multivariate analysis for selection of metabolites with variable importance for the projection (VIP) > 1, t-test p value < 0...
January 10, 2017: Toxins
https://www.readbyqxmd.com/read/28058725/urinary-metabolomics-of-complete-freund-s-adjuvant-induced-hyperalgesia-in-rats
#16
Yang Liu, Hui Chen, Jun Lu, Youshui Jiang, Rui Yang, Songyan Gao, Xin Dong, Wei Chen
The aim of this study was to demonstrate the differences of metabolomics changes in a hyperalgesia model and find potent biomarkers of hyperalgesia. Seven rats were placed in metabolic cages. An emulsion containing 500 μg of Complete Freund's adjuvant (CFA) was used to induce hyperalgesia. Urine samples were collected prior to the injection of CFA and on post-injection days 1, 3 and 7. Ultraperformance liquid chromatography, coupled with quadrupole-time-of-flight mass spectrometry (UHPLC-Q-TOF/MS), was used for a quantitative analysis of urinary metabolic changes in the CFA-induced hyperalgesia model...
November 5, 2016: Biomedical Chromatography: BMC
https://www.readbyqxmd.com/read/28053823/global-metabolic-profiling-of-plasma-shows-that-three-year-mild-caloric-restriction-lessens-an-age-related-increase-in-sphingomyelin-and-reduces-l-leucine-and-l-phenylalanine-in-overweight-and-obese-subjects
#17
Minjoo Kim, Sang-Hyun Lee, Jong Ho Lee
The effect of weight loss from long-term, mild-calorie diets (MCD) on plasma metabolites is unknown. This study was to examine whether MCD-induced weight reduction caused changes in the extended plasma metabolites. Overweight and obese subjects aged 40-59 years consumed a MCD (approximately 100 kcal/day deficit, n=47) or a weight-maintenance diet (control, n=47) in a randomized, controlled design with a three-year clinical intervention period and plasma samples were analyzed by using UPLC-LTQ-Orbitrap mass spectrometry...
December 2016: Aging and Disease
https://www.readbyqxmd.com/read/28013555/primary-and-secondary-metabolites-of-an-european-edible-mushroom-and-its-nutraceutical-value-suillus-bellinii-inzenga-kuntze
#18
Alessandro Venditti, Claudio Frezza, Fabio Sciubba, Mauro Serafini, Armandodoriano Bianco
In this work, we report the study on the composition in primary and secondary metabolites of the polar and less-polar fractions obtained from the fruiting bodies of Suillus bellinii (Inzenga) Kuntze collected from Central Italy. Thirteen compounds were identified through a series of classical chromatographic methods and through spectroscopic techniques (nuclear magnetic resonance (NMR) and mass spectrometry (MS)), namely suillin (1), ergosterol (2), glutamic acid (3), isoleucine (4), leucine (5), threonine (6), tyrosine (7), valine (8), D-mannitol (9), sucrose (10), D-sorbitol (11), fumaric acid (12) and alanine (13)...
December 26, 2016: Natural Product Research
https://www.readbyqxmd.com/read/28000843/wheat-bran-autolytic-peptides-containing-a-branched-chain-amino-acid-attenuate-non-alcoholic-steatohepatitis-via-the-suppression-of-oxidative-stress-and-the-upregulation-of-ampk-acc-in-high-fat-diet-fed-mice
#19
Takumi Kawaguchi, Takato Ueno, Yoichi Nogata, Masako Hayakawa, Hironori Koga, Takuji Torimura
Whole-wheat intake is known to reduce the risk of metabolic syndrome. However, the active component remains unclear. Recently, we identified bioactive peptides [leucine-arginine-proline (LRP) and leucine-glutamine‑proline (LQP)] from wheat bran autolytic hydrolysate. The present study aimed to investigate the effects of LRP and LQP on non-alcoholic steatohepatitis (NASH) in a mouse model. We also evaluated the effects of these peptides on oxidative stress and on the AMP-activated protein kinase (AMPK) signaling pathway, two major pathogenic factors of NASH...
February 2017: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/27990605/profiling-of-selected-functional-metabolites-in-the-central-nervous-system-of-marine-medaka-oryzias-melastigma-for-environmental-neurotoxicological-assessments
#20
Elva Ngai-Yu Lei, Man-Shan Yau, Chi-Chung Yeung, Margaret B Murphy, Ka-Leung Wong, Michael Hon-Wah Lam
The simultaneous profiling of 43 functional metabolites in the brain of the small model vertebrate organism, marine medaka (Oryzais melastigma), has been accomplished via dansyl chloride derivatization and LC-MS/MS quantification. This technique was applied to examine effects of 2,2',4,4'-tetrabromodiphenyl ether (BDE-47), one of the most abundant polybrominated diphenyl ether flame retardants in the natural environment, on the central nervous system (CNS) of vertebrates. The model teleosts were fed with bioencapsulated Artemia nauplii for up to 21 days...
February 2017: Archives of Environmental Contamination and Toxicology
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