Katrine Bugge, Lasse Staby, Katherine R Kemplen, Charlotte O'Shea, Sidsel K Bendsen, Mikael K Jensen, Johan G Olsen, Karen Skriver, Birthe B Kragelund
Communication within cells relies on a few protein nodes called hubs, which organize vast interactomes with many partners. Frequently, hub proteins are intrinsically disordered conferring multi-specificity and dynamic communication. Conversely, folded hub proteins may organize networks using disordered partners. In this work, the structure of the RST domain, a unique folded hub, is solved by nuclear magnetic resonance spectroscopy and small-angle X-ray scattering, and its complex with a region of the transcription factor DREB2A is provided through data-driven HADDOCK modeling and mutagenesis analysis...
April 12, 2018: Structure