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Mohammed A El-Magd, Abeer Khamis, Samah K Nasr Eldeen, Wafaa M Ibrahim, Afrah F Salama
Methotrexate (MTX) is commonly used as a standard chemotherapy for many cancers, however its usage required high doses thereby leading to severe adverse effects. In a trial to find a suitable neoadjuvant therapy to decrease MTX dosage without lowering its chemotherapeutic efficacy, we investigated the antitumor effect of trehalose (TRE) on mice bearing Ehrlich ascites carcinoma (EAC) and checked whether TRE can enhance the antitumor potential of MTX. Treatment with TRE induced anti-tumor effects against EAC as reveled by a remarkable decrease in body weight, tumor volume, count of viable tumor cells, expression of the anti-apoptotic gene Bcl2 as well as by a significant increase in mean survival time, life span and expression of the apoptotic gene caspase-3...
August 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
Shinji Takahashi, Kohei Inamura, Junko Yarimizu, Mayako Yamazaki, Nobuhito Murai, Keni Ni
KCNH3 (BEC1) is a member of the ether-à-go-go (KCNH) family of voltage-gated K(+) channels. The aim of this study was to determine the pharmacological profiles in vitro and in vivo of a KCNH3 inhibitor N-(4-fluorophenyl)-N'-phenyl-N''-(pyrimidin-2-ylmethyl)-1,3,5-triazine-2,4,6-triamine (ASP2905). We analyzed the effects of ASP2905 on channel activity in vitro and its neuropharmacological properties in young and aged rats as well as in mice. ASP2905 potently inhibited potassium currents in CHO cells expressing KCNH3 (IC50 = 9...
September 5, 2017: European Journal of Pharmacology
Yi Wang, Yao-Xin Lin, Zeng-Ying Qiao, Hong-Wei An, Sheng-Lin Qiao, Lei Wang, R P Yeshan J Rajapaksha, Hao Wang
A peptide-conjugated poly(β-amino ester) that self-assembles into micelle-like nanoparticles is prepared by a convenient and modular supramolecular approach. The polymer-beclin-1 (P-Bec1) nanoparticles display enhanced cytotoxicity to breast cancer cells through induction of autophagy. This approach overcomes two major limitations of the haploinsufficient tumor suppressor Bec1 compared to small-molecule drugs: poor delivery to tumors owing to enzymatic degradation, and unstable, non-specific bio-distribution and targeting in the tumor tissues...
April 24, 2015: Advanced Materials
Yutaka Tomizawa, Prasad G Iyer, Louis M Wongkeesong, Navtej S Buttar, Lori S Lutzke, Tsung-Teh Wu, Kenneth K Wang
AIM: To investigate a classification of endocytoscopy (ECS) images in Barrett's esophagus (BE) and evaluate its diagnostic performance and interobserver variability. METHODS: ECS was applied to surveillance endoscopic mucosal resection (EMR) specimens of BE ex-vivo. The mucosal surface of specimen was stained with 1% methylene blue and surveyed with a catheter-type endocytoscope. We selected still images that were most representative of the endoscopically suspect lesion and matched with the final histopathological diagnosis to accomplish accurate correlation...
December 14, 2013: World Journal of Gastroenterology: WJG
Sarah M Schmidt, Hannah Kuhn, Cristina Micali, Corinna Liller, Mark Kwaaitaal, Ralph Panstruga
Filamentous phytopathogens, such as fungi and oomycetes, secrete effector proteins to establish successful interactions with their plant hosts. In contrast with oomycetes, little is known about effector functions in true fungi. We used a bioinformatics pipeline to identify Blumeria effector candidates (BECs) from the obligate biotrophic barley powdery mildew pathogen, Blumeria graminis f. sp. hordei (Bgh). BEC1-BEC5 are expressed at different time points during barley infection. BEC1, BEC2 and BEC4 have orthologues in the Arabidopsis thaliana-infecting powdery mildew fungus Golovinomyces orontii...
August 2014: Molecular Plant Pathology
Madeleine Herrmann, Klemens Ruprecht, Marlies Sauter, Javier Martinez, Pearl van Heteren, Michael Glas, Barbara Best, Andreas Meyerhans, Klaus Roemer, Nikolaus Mueller-Lantzsch
Retrovirus replication critically depends on a dynamic interplay between retroviral and host proteins. We report on the binding of the surface subunit (glycoprotein 120 (gp120)) of the human immunodeficiency virus type 1 (HIV-1) envelope protein (Env) to the cytoplasmic C-terminus of the voltage-gated potassium channel BEC1 (brain-specific ether-a-go-go-like channel 1), an interaction that can result in the repression of BEC's activity and the inhibition of HIV-1 particle-release. BEC1 protein was found to be expressed in T cells and macrophages, the major target cells of HIV-1...
August 20, 2010: FEBS Letters
Maria Chiara Maiuri, Alfredo Criollo, Guido Kroemer
Although the essential genes for autophagy (Atg) have been identified, the molecular mechanisms through which Atg proteins control 'self eating' in mammalian cells remain elusive. Beclin 1 (Bec1), the mammalian orthologue of yeast Atg6, is part of the class III phosphatidylinositol 3-kinase (PI3K) complex that induces autophagy. The first among an increasing number of Bec1-interacting proteins that has been identified is the anti-apoptotic protein Bcl-2. The dissociation of Bec1 from Bcl-2 is essential for its autophagic activity, and Bcl-2 only inhibits autophagy when it is present in the endoplasmic reticulum (ER)...
February 3, 2010: EMBO Journal
Akira Miyake, Shinji Takahashi, Yukihiro Nakamura, Kohei Inamura, Shun-Ichiro Matsumoto, Shinobu Mochizuki, Masao Katou
The K+ channel, one of the determinants for neuronal excitability, is genetically heterogeneous, and various K+ channel genes are expressed in the CNS. The therapeutic potential of K+ channel blockers for cognitive enhancement has been discussed, but the contribution each K+ channel gene makes to cognitive function remains obscure. BEC1 (KCNH3) is a member of the K+ channel superfamily that shows forebrain-preferential distribution. Here, we show the critical involvement of BEC1 in cognitive function. BEC1 knock-out mice performed behavioral tasks related to working memory, reference memory, and attention better than their wild-type littermates...
November 18, 2009: Journal of Neuroscience: the Official Journal of the Society for Neuroscience
Said Amrani, Nazhat-Ezzaman Noureddine, Tej Bhatnagar, Montserrat Argandoña, Joaquin J Nieto, Carmen Vargas
Twenty seven rhizobial strains associated with Acacia saligna grown in northern and southern Algeria were characterized, including generation time, host-range, the 16S rRNA gene and 16S-23S rRNA intergenic spacer restriction patterns, 16S rRNA gene sequence analysis and tolerance to salinity and drought. Cross inoculation tests indicated that 11 slow-growing isolates from northern nurseries were able to nodulate introduced Australian acacias exclusively, whereas 16 fast-growing isolates, mainly from southern nurseries, were capable of also nodulating native acacias...
January 2010: Systematic and Applied Microbiology
Richard A Radcliffe, V Gene Erwin, Laura Draski, Sarah Hoffmann, Joel Edwards, Xin-Sheng Deng, Pequita Bludeau, Tina Fay, Kristy Lundquist, William Asperi, Richard A Deitrich
BACKGROUND: Genetic variance in initial sensitivity to ethanol has been implicated as a risk factor for the development of alcoholism. Identification of the genes that confer differential initial sensitivity is an important goal for the development of new treatment strategies and for a comprehensive understanding of the mechanism of ethanol's action. Quantitative trait loci (QTL) mapping for initial sensitivity and other ethanol-related behavioral traits in model organisms has become an important first step for the ultimate identification of genes that contribute to variation in ethanol responses...
December 2004: Alcoholism, Clinical and Experimental Research
Garth A M Smith, Hing-Wo Tsui, Evan W Newell, Xinpo Jiang, Xiao-Ping Zhu, Florence W L Tsui, Lyanne C Schlichter
Kv1.3 channels regulate proliferation of normal lymphocytes, but the role of voltage-gated potassium channels in transformed hematopoietic cells is not known. We examined transcripts for Kv1.3, h-erg, h-eag, and BEC1 genes in primary lymphocytes and leukemias and in several hematopoietic cell lines. Surprisingly, BEC1, formerly thought to be brain-specific, was present in all the primary leukemias examined, in resting peripheral blood lymphocytes, and in proliferating activated tonsillar cells, lymphocytes from Sjögren's patients, and Epstein-Barr virus-transformed B-cells...
May 24, 2002: Journal of Biological Chemistry
S L Kirstein, B Tabakoff
BACKGROUND: Several lines of evidence have suggested a role for cAMP (adenosine 3',5'-cyclic monophosphate) signaling in the acute and chronic effects of ethanol. This study investigated whether there is a genetic correlation between cAMP synthesis in the brain and the acute effects of ethanol [alcohol sensitivity or acute functional tolerance (AFT)]. METHODS: By using nine inbred strains of mice, we measured initial sensitivity and AFT to ethanol with a test of balance on a dowel...
June 2001: Alcoholism, Clinical and Experimental Research
A Miyake, S Mochizuki, H Yokoi, M Kohda, K Furuichi
A cDNA encoding a novel voltage-gated K(+) channel protein was isolated from human brain. This protein, termed BEC1, is 46% identical to rat elk in the ether-à-go-go K(+) channel family. The BEC1 gene maps to the 12q13 region of the human genome. Northern blot analysis indicates that BEC1 is exclusively expressed in human brain, where the expression is concentrated in the telencephalic areas such as the cerebral cortex, amygdala, hippocampus, and striatum. By in situ hybridization, BEC1 is detected in the CA1-CA3 pyramidal cell layers and the dentate gyrus granule cell layers of the hippocampus...
August 27, 1999: Journal of Biological Chemistry
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