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Peripheral blood morphology

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https://www.readbyqxmd.com/read/28333411/utility-of-peripheral-blood-immunophenotyping-by-flow-cytometry-in-the-diagnosis-of-pediatric-acute-leukemia
#1
Laura K Metrock, Ryan J Summers, Sunita Park, Scott Gillespie, Sharon Castellino, Glen Lew, Frank G Keller
BACKGROUND: Childhood acute leukemia is traditionally diagnosed from a bone marrow aspirate (BMA). New-onset acute leukemia patients do not always have visible circulating blasts in the peripheral blood (PB) at diagnosis. While the role of bone marrow flow cytometry for the diagnosis of acute leukemia is well established, the utility of PB flow cytometry (PBFC) is unknown. We performed a single-institution retrospective analysis to compare PBFC versus BMA in establishing or excluding a diagnosis of childhood acute leukemia...
March 23, 2017: Pediatric Blood & Cancer
https://www.readbyqxmd.com/read/28329397/resveratrol-improves-vascular-function-and-mitochondrial-number-but-not-glucose-metabolism-in-older-adults
#2
Rena M Pollack, Nir Barzilai, Valentin Anghel, Ameya S Kulkarni, Aaron Golden, Pilib O'Broin, David A Sinclair, Michael S Bonkowski, Alexander J Coleville, Danielle Powell, Sharon Kim, Ruin Moaddel, Daniel Stein, Kehao Zhang, Meredith Hawkins, Jill P Crandall
Background: Resveratrol, a plant-derived polyphenol, has been reported to improve glucose metabolism and vascular function and to extend life span in animal models, but studies in humans have been inconclusive. Methods: In a randomized, double-blind crossover study, we treated older glucose-intolerant adults (n = 30) with resveratrol (2-3 g/daily) or placebo, each for 6 weeks. A standard mixed-meal test was used to assess insulin sensitivity (Matsuda index) and secretion (C-peptide deconvolution) and vascular function by reactive hyperemia peripheral arterial tonometry...
March 16, 2017: Journals of Gerontology. Series A, Biological Sciences and Medical Sciences
https://www.readbyqxmd.com/read/28324193/hemodynamic-characterization-of-peripheral-arterio-venous-malformations
#3
Sabrina Frey, A Haine, R Kammer, H von Tengg-Kobligk, D Obrist, I Baumgartner
Peripheral arterio-venous malformations (pAVMs) are congenital vascular anomalies that require treatment, due to their severe clinical consequences. The complexity of lesions often leads to misdiagnosis and ill-planned treatments. To improve disease management, we developed a computational model to quantify the hemodynamic effects of key angioarchitectural features of pAVMs. Hemodynamic results were used to predict the transport of contrast agent (CA), which allowed us to compare our findings to digital subtraction angiography (DSA) recordings of patients...
March 21, 2017: Annals of Biomedical Engineering
https://www.readbyqxmd.com/read/28321092/refractory-double-hit-lymphoma-leukemia-in-childhood-mimicking-b-precursor-acute-lymphoblastic-leukemia-at-initial-presentation
#4
Suguru Uemura, Daiichiro Hasegawa, Takehito Yokoi, Nanako Nino, Teppei Tahara, Akihiro Tamura, Atsuro Saito, Aiko Kozaki, Kenji Kishimoto, Toshiaki Ishida, Keiichiro Kawasaki, Nobuyuki Yamamoto, Takeshi Mori, Noriyuki Nishimura, Yoshiyuki Kosaka
A 10-year-old girl was referred to our hospital with left preauricular adenopathy and gingival swelling. She was diagnosed with B-cell precursor acute lymphoblastic leukemia (BCP-ALL) based on being positive for expressions of CD10, CD19, TdT and HLA-DR. She showed no CD20 expression at the time of diagnosis. Based on the initial diagnosis of BCP-ALL, induction chemotherapy for BCP-ALL was initiated. However, the blasts did not disappear from her peripheral blood. Bone marrow examination on day 33 identified 81...
2017: [Rinshō Ketsueki] the Japanese Journal of Clinical Hematology
https://www.readbyqxmd.com/read/28319827/immunophenotypical-characterization-of-canine-mesenchymal-stem-cells-from-perivisceral-and-subcutaneous-adipose-tissue-by-a-species-specific-panel-of-antibodies
#5
Ana Ivanovska, Stefano Grolli, Paolo Borghetti, Francesca Ravanetti, Virna Conti, Elena De Angelis, Francesca Macchi, Roberto Ramoni, Paolo Martelli, Ferdinando Gazza, Antonio Cacchioli
Immunophenotypical characterization of mesenchymal stem cells is fundamental for the design and execution of sound experimental and clinical studies. The scarce availability of species-specific antibodies for canine antigens has hampered the immunophenotypical characterization of canine mesenchymal stem cells (MSC). The aim of this study was to select a panel of species-specific direct antibodies readily useful for canine mesenchymal stem cells characterization. They were isolated from perivisceral and subcutaneous adipose tissue samples collected during regular surgeries from 8 dogs...
February 27, 2017: Research in Veterinary Science
https://www.readbyqxmd.com/read/28319139/identification-of-cell-morphology-parameters-from-automatic-hematology-analyzers-to-predict-the-peripheral-blood-cd34-positive-cell-count-after-mobilization
#6
Saeam Shin, Sung Ran Cho, Sinyoung Kim, Jong Rak Choi, Kyung-A Lee
Optimal timing of apheresis initiation is important for maximizing the hematopoietic stem cell (HSC) yield. This study aimed to identify useful parameters from automatic hematology analyzers for predicting the peripheral blood CD34+ cell count after mobilization. We prospectively enrolled 53 healthy donors and 72 patients, and evaluated 43 cell morphology parameters from Unicel DxH800 (Beckman Coulter, USA) and Advia 2120i (Siemens Healthcare Diagnostics, USA). The correlation of each parameter with the CD34+ cell count in pre-apheresis blood samples was analyzed...
2017: PloS One
https://www.readbyqxmd.com/read/28315720/the-protective-effect-of-the-active-components-of-erpc-on-diabetic-peripheral-neuropathy-in-rats
#7
Gai-Mei Hao, Yong-Gang Liu, Yan Wu, Wei Xing, Shu-Zhen Guo, Yong Wang, Zheng-Lin Wang, Chun Li, Tian-Tian Lv, Hong-Liang Wang, Tian-Jiao Shi, Wei Wang, Jing Han
ETHNOPHARMACOLOGICAL RELEVANCE: Euonymus alatus, Radix trichosanthis, Panax notoginseng and Coptis chinensis are popular plants used in traditional Chinese medicine to treat diabetes. AIM OF THE STUDY: The aim of the study is to investigate the therapeutic effect of the active components of Euonymus alatus, Radix trichosanthis, Panax notoginseng and Coptis chinensis (cERPC) on diabetic peripheral neuropathy in the rats and explore the underlying mechanism involved...
March 15, 2017: Journal of Ethnopharmacology
https://www.readbyqxmd.com/read/28297240/analytical-comparison-between-two-hematological-analyzer-systems-cal-8000-vs-xn-9000
#8
COMPARATIVE STUDY
S Buoro, T Mecca, M Seghezzi, B Manenti, G Azzarà, P Dominoni, A Crippa, C Ottomano, G Lippi
INTRODUCTION: This study was aimed to compare the analytical performance of traditional and new parameters and morphological flags of CAL-8000 and XN-9000. The automated differential leukocyte count (DIFF profile) and morphological flags were compared with optical microscopy (OM). METHODS: A total of 1025 peripheral blood samples, collected in K3 EDTA tubes, were analyzed by CAL-8000, by XN-9000, and by OM. Within-run imprecision was performed in low cellularity samples...
April 2017: International Journal of Laboratory Hematology
https://www.readbyqxmd.com/read/28287078/the-design-of-and-chronic-tissue-response-to-a-composite-nerve-electrode-with-patterned-stiffness
#9
Max Freeberg, Matthew Stone, Ronald Triolo, Dustin Tyler
As neural interfaces demonstrate success in chronic applications, a novel class of reshaping electrodes with patterned regions of stiffness will enable application to a widening range of anatomical locations. Patterning stiff regions and flexible regions of the electrode enables nerve reshaping while accommodating anatomical constraints of various implant locations ranging from peripheral nerves to spinal and autonomic plexi. Introduced is a new composite electrode enabling patterning of regions of various electrode mechanical properties...
March 13, 2017: Journal of Neural Engineering
https://www.readbyqxmd.com/read/28285346/complex-changes-in-the-innate-and-adaptive-immunity-accompany-progressive-degeneration-of-the-nigrostriatal-pathway-induced-by-intrastriatal-injection-of-6-hydroxydopamine-in-the-rat
#10
Giulia Ambrosi, Natasa Kustrimovic, Francesca Siani, Emanuela Rasini, Silvia Cerri, Cristina Ghezzi, Giuseppe Dicorato, Sofia Caputo, Franca Marino, Marco Cosentino, Fabio Blandini
We investigated changes in innate and adaptive immunity paralleling the progressive nigrostriatal damage occurring in a neurotoxic model of Parkinson's disease (PD) based on unilateral infusion of 6-hydroxydopamine (6-OHDA) into the rat striatum. A time-course analysis was conducted to assess changes in morphology (activation) and cell density of microglia and astrocytes, microglia polarization (M1 vs. M2 phenotype), lymphocyte infiltration in the lesioned substantia nigra pars compacta (SNc), and modifications of CD8+ and subsets of CD4+ T cell in peripheral blood accompanying nigrostriatal degeneration...
March 11, 2017: Neurotoxicity Research
https://www.readbyqxmd.com/read/28283696/myeloid-cells-in-circulation-and-tumor-microenvironment-of-breast-cancer-patients
#11
Salman M Toor, Azharuddin Sajid Syed Khaja, Haytham El Salhat, Issam Faour, Jihad Kanbar, Asif A Quadri, Mohamed Albashir, Eyad Elkord
Pathological conditions including cancers lead to accumulation of a morphological mixture of highly immunosuppressive cells termed as myeloid-derived suppressor cells (MDSC). The lack of conclusive markers to identify human MDSC, due to their heterogeneous nature and close phenotypical and functional proximity with other cell subsets, made it challenging to identify these cells. Nevertheless, expansion of MDSC has been reported in periphery and tumor microenvironment of various cancers. The majority of studies on breast cancers were performed on murine models and hence limited literature is available on the relation of MDSC accumulation with clinical settings in breast cancer patients...
March 10, 2017: Cancer Immunology, Immunotherapy: CII
https://www.readbyqxmd.com/read/28282510/cxcr4-cd184-expression-on-stem-cell-harvest-and-cd34-cells-post-transplant
#12
Inas Asfour, Hanaa Afify, Shaza Elkourashy, Maryse Ayoub, Gihan Kamal, Mary Gamal, Ghada Elgohary
OBJECTIVES/BACKGROUND: CXCR4 is a receptor for stromal-derived factor-1 (SDF-1), a molecule that has a chemotactic activity for lymphocytes and is important in homing of hematopoietic stem cells to their adult marrow. We evaluated the CXCR4 (CD184) expression in the harvest cells and in the post-transplant bone marrow (BM) and its relation to engraftment, as determined by the consensus criteria and chimerism. METHODS: This is a prospective study which included 30 patients undergoing hematopoietic stem cell transplantation; 15 patients received autograft and 15 patients received allograft on dates between January 2012 and May 2014...
March 2, 2017: Hematology/oncology and Stem Cell Therapy
https://www.readbyqxmd.com/read/28280089/successful-transfer-of-umbilical-cord-blood-cd34-hematopoietic-stem-and-progenitor-derived-nk-cells-in-older-acute-myeloid-leukemia-patients
#13
Harry Dolstra, Mieke W H Roeven, Jan Spanholtz, Basav N Hangalapura, Marleen Tordoir, Frans Maas, Marij Leenders, Fenna Bohme, Nina Kok, Carel Trilsbeek, Jos Paardekooper, Anniek B van der Waart, Peter E Westerweel, Tjeerd J F Snijders, Jan J Cornelissen, Gerard M J Bos, Hans F M Pruijt, Aniek O De Graaf, Bert van der Reijden, Joop H Jansen, Arnold van der Meer, Gerwin Huls, Jeannette Cany, Frank Preijers, Nicole M A Blijlevens, Nicolaas M Schaap
PURPOSE: Older acute myeloid leukemia (AML) patients have a poor prognosis, therefore novel therapies are needed. Allogeneic natural killer (NK) cells have been adoptively transferred with promising clinical results. Here, we report the first-in-human study exploiting an unique scalable NK cell product generated ex vivo from CD34+ hematopoietic stem and progenitor cells (HSPCs) from partially HLA-matched umbilical cord blood units. EXPERIMENTAL DESIGN: Ten older AML patients in morphologic complete remission received an escalating HSPC-NK cell dose (between 3 and 30x10^6/kg body weight) after lymphodepleting chemotherapy without cytokine boosting...
March 9, 2017: Clinical Cancer Research: An Official Journal of the American Association for Cancer Research
https://www.readbyqxmd.com/read/28276721/relationship-between-different-subpopulations-of-circulating-cd4-t-lymphocytes-and-microvascular-or-systemic-oxidative-stress-in-humans
#14
Carolina De Ciuceis, Claudia Agabiti-Rosei, Claudia Rossini, Paolo Airò, Mirko Scarsi, Angela Tincani, Guido Alberto Massimo Tiberio, Silvia Piantoni, Enzo Porteri, Leonardo Solaini, Sarah Duse, Francesco Semeraro, Beatrice Petroboni, Luigi Mori, Maurizio Castellano, Alice Gavazzi, Enrico Agabiti-Rosei, Damiano Rizzoni
BACKGROUND AND OBJECTIVE: Different components of the immune system, including innate and adaptive immunity (T effector lymphocytes and T regulatory lymphocytes - TREGs) may be involved in the development of hypertension, vascular injury and inflammation. However, no data are presently available in humans about possible relationships between T-lymphocyte subtypes and microvascular oxidative stress. Our objective was to investigate possible relationships between T-lymphocyte subtypes and systemic and microvascular oxidative stress in a population of normotensive subjects and hypertensive patients...
February 15, 2017: Blood Pressure
https://www.readbyqxmd.com/read/28267648/expression-of-soluble-cd83-in-plasma-from-early-stage-rheumatoid-arthritis-patients-is-not-modified-by-anti-tnf-%C3%AE-therapy
#15
Anne-Mette Kristensen, Kristian Stengaard-Pedersen, Merete Lund Hetland, Kim Hørslev-Petersen, Peter Junker, Mikkel Østergaard, Per Höllsberg, Bent Deleuran, Malene Hvid
Rheumatoid arthritis (RA) is an autoimmune disease which may lead to severe disabilities due to structural joint damage and extraarticular manifestations The dendritic cell marker CD83 belongs to the immunoglobulin superfamily and has previously been associated with autoimmune diseases. In RA the levels of soluble CD83 (sCD83) are elevated in synovial fluid, however little is known about CD83 expression and regulation in RA. Therefore, we studied how CD83 is expressed in RA and further evaluated the effect of anti-TNF-α therapy hereon...
March 3, 2017: Cytokine
https://www.readbyqxmd.com/read/28261866/morphologic-and-immunophenotypic-features-of-hairy-cell-leukemia-involving-lymph-nodes-and-extranodal-tissues
#16
Jacqueline M Cortazar, Daniel J DeAngelo, Geraldine S Pinkus, Elizabeth A Morgan
Hairy cell leukemia (HCL) is a rare B-cell neoplasm that mainly affects bone marrow (BM), peripheral blood (PB), and spleen. Involvement of lymph nodes and extranodal structures is considered infrequent. Herein we describe our institutional experience of nodal (n=10) and extranodal (n=3) HCL over a thirty-year period. Ten patients had prior evidence of HCL within the BM or PB at a median 35.8 months before nodal/extranodal diagnosis (range, <1 to 175 months), and HCL was diagnosed concurrently within the bone marrow of 1 additional patient...
March 6, 2017: Histopathology
https://www.readbyqxmd.com/read/28245368/-clinical-and-immunophenotypic-properties-of-small-cell-variant-of-t-cell-prolymphocytic-leukemia
#17
Ya-Ping Yu, Li-Ping Wang, Ping Song, Jian-Gang Mei, Zhi-Ming An, Xiao-Gang Zhou, Feng Li, Yu-Mei Tang, Yong-Ping Zhai
OBJECTIVE: To investigate the clinical, morphologic and immunophenotypic properties of the patients with small cell variant of T-cell prolymphocytic leukaemia(T-PLL). METHODS: Peripheral blood and bone marrow cytomorphologic and immunophenotypic examination, and T-cell receptor(TCR) gene rearrangement detection were used to verify the diagnosis for 2 patients with lymphocytosis. Two patients were treated with combined chemotherapeutic protocol based on fludarabine...
February 2017: Zhongguo Shi Yan Xue Ye Xue za Zhi
https://www.readbyqxmd.com/read/28242986/mixed-phenotypic-acute-leukemia-mixed-myeloid-b-cell-with-myeloid-sarcoma-of-the-thyroid-gland-a-rare-entity-with-rarer-asssociation-detected-on-fdg-pet-ct
#18
Gaurav Khanna, Nishikant Avinash Damle, Shipra Agarwal, Maitrayee Roy, Deepali Jain, Soumyaranjan Mallick, Shamim Ahmed, Madhavi Tripathi, Ajay Gogia
Mixed phenotypic acute leukemia (MPAL) is a rare clinical entity. MPAL associated with myeloidsarcoma (MS) is still rarer with only three cases mentioned in English literature. MS has been described in myriads of location, most commonly in skin, gums and lymph nodes. Although theoritically possible, it is very rare to find MS involving the thyroid gland. The diagnosis of MS can be elusive, very often masquerades and mislabeled as lymphoma. A high index of clinical suspicion coupled with PET/CT findings along with morphological clues and thorough peripheral blood, and bone marrow evaluation is mandatory for arriving at the definitive diagnosis...
January 2017: Indian Journal of Nuclear Medicine: IJNM: the Official Journal of the Society of Nuclear Medicine, India
https://www.readbyqxmd.com/read/28242497/toxicological-effects-of-graphene-oxide-on-adult-zebrafish-danio-rerio
#19
Jaqueline P Souza, Jéssica F Baretta, Fabrício Santos, Ieda M M Paino, Valtencir Zucolotto
Graphene exhibits unique physical and chemical properties that facilitate its application in many fields, including electronics and biomedical areas. However, the use of graphene and its derivatives could result in accumulation in aquatic environments, and the risks posed by these compounds for organisms are not completely understood. In this study, we investigated the effects of graphene oxide (GO) on adult zebrafish (Danio rerio). Experimental fish were exposed to 2, 10 or 20mgL(-1) GO, and the cytotoxicity, genotoxicity and oxidative stress were assessed...
February 16, 2017: Aquatic Toxicology
https://www.readbyqxmd.com/read/28203345/sweet-s-syndrome-associated-with-clonal-hematopoiesis-of-indeterminate-potential-responsive-to-5-azacitidine
#20
REVIEW
George Yaghmour, Eric Wiedower, Bassam Yaghmour, Sara Nunnery, Eric Duncavage, Mike G Martin
Sweet's syndrome (SS) is a rare condition characterized by the abrupt appearance of painful skin lesions due to neutrophilic dermal infiltration. Hematologic neoplasms, particularly acute myeloid leukemia (AML) and myelodysplastic syndromes (MDSs), have been commonly reported in association with SS. Clonal hematopoiesis of indeterminate potential (CHIP) is an emerging entity that is a precursor state to myeloid neoplasms. CHIP has not been previously associated with SS. We report the case of a 71-year-old man who presented with recurrent, painful edematous and erythematous papules and nodules for 18 months despite treatment with corticosteroids...
February 2017: Therapeutic Advances in Hematology
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