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https://www.readbyqxmd.com/read/28543440/chronic-sleep-restriction-disrupts-interendothelial-junctions-in-the-hippocampus-and-increases-blood-brain-barrier-permeability
#1
G Hurtado-Alvarado, J Velázquez-Moctezuma, B Gómez-González
Chronic sleep loss in the rat increases blood-brain barrier permeability to Evans blue and FITC-dextrans in almost the whole brain and sleep recovery during short periods restores normal blood-brain barrier permeability. Sleep loss increases vesicle density in hippocampal endothelial cells and decreases tight junction protein expression. However, at the ultrastructural level the effect of chronic sleep loss on interendothelial junctions is unknown. In this study we characterised the ultrastructure of interendothelial junctions in the hippocampus, the expression of tight junction proteins, and quantified blood-brain barrier permeability to fluorescein-sodium after chronic sleep restriction...
May 23, 2017: Journal of Microscopy
https://www.readbyqxmd.com/read/28540178/scanometry-as-microplate-reader-for-high-throughput-method-based-on-dpph-dry-reagent-for-antioxidant-assay
#2
Mochammad Amrun Hidayat, Aulia Fitri, Bambang Kuswandi
The stable chromogenic radical 1,1'-diphenyl-2-picrylhydrazyl (DPPH) solution was immobilized on the microwell plate as dry reagent to construct a simple antioxidant sensor. Then, a regular flatbed scanner was used as microplate reader to obtain analytical parameters for antioxidant assay using one-shot optical sensors as scanometry technique. Variables affecting the acquisition of the images were optimized and the analytical parameters are obtained from an area of the sensing zone inside microwell using the average luminosity of the sensing zone captured as the mean of red, green, and blue (RGB) value using ImageJ(®) program...
May 2017: Acta Pharmaceutica Sinica. B
https://www.readbyqxmd.com/read/28532438/the-effect-of-color-type-on-early-wound-healing-in-farmed-mink-neovison-vison
#3
A Jespersen, H E Jensen, J F Agger, P M H Heegaard, P Damborg, B Aalbæk, A S Hammer
BACKGROUND: Individual differences of mink, including color type, are speculated to affect the course of wound healing, thereby impacting wound assessment and management on the farms, as well as the assessment of wounds in forensic cases. In this study, we examined the effect of color type on early wound healing in farmed mink. Full thickness excisional wounds (2 × 2 cm) were made on the back in 18 mink of the color types Brown, Silverblue and Blue Iris. Gross and microscopic pathology of the wounds was evaluated 2 days post-wounding together with degree of wound size reduction, presence of bacteria and blood analyses...
May 22, 2017: BMC Veterinary Research
https://www.readbyqxmd.com/read/28530199/synthesis-of-ag-nps-impregnated-cellulose-composite-material-its-possible-role-in-wound-healing-and-photocatalysis
#4
Attarad Ali, Ihsan Ul Haq, Javeed Akhtar, Muhammad Sher, Naveed Ahmed, Muhammad Zia
Cellulose is the natural biopolymer normally used as supporting agent with enhanced applicability and properties. In present study, cellulose isolated from citrus waste is used for silver nanoparticles (Ag-NPs) impregnation by a simple and reproducible method. The Ag-NPs fabricated cellulose (Ag-Cel) was characterised by powder X-rays diffraction, Fortier transform infrared spectroscopy and scanning electron microscopy. The thermal stability was studied by thermo-gravimetric analysis. The antibacterial activity performed by disc diffusion assay reveals good zone of inhibition against Staphylococcus aureus and Escherichia coli by Ag-Cel as compared Ag-NPs...
June 2017: IET Nanobiotechnology
https://www.readbyqxmd.com/read/28529654/histological-histochemical-and-ultrastructural-study-of-slipped-capital-femoral-epiphysis
#5
I Tresoldi, A Modesti, M Dragoni, V Potenza, E Ippolito
PURPOSE: The purpose of our study was to investigate the histological, histochemical and ultrastructural aspects of the proximal femoral growth plate in slipped capital femoral epiphysis (SCFE). METHODS: Eight core biopsies of the proximal femoral growth plate were performed during in situ epiphysiodesis in patients with SCFE that was at the pre-slipping stage in two cases and at the mild slipping stage (Southwick angle < 30°) in six cases. After fixation, the specimens were processed for either histological or histochemical or ultrastructural studies...
April 2017: Journal of Children's Orthopaedics
https://www.readbyqxmd.com/read/28510785/the-impact-of-leisure-on-mental-health-of-sardinian-elderly-from-the-blue-zone-evidence-for-ageing-well
#6
Maria Chiara Fastame, Paul Kenneth Hitchcott, Maria Pietronilla Penna
BACKGROUND: There has been scientific interest in the Sardinia population for a number of years and for a variety of reasons, including its extreme longevity. This is especially prevalent in the central-eastern region of the island, where a blue zone region has been precisely delineated. AIMS: The present study examines the influence of leisure activities on psychological well-being and depressive symptoms in elderly individuals living in the blue zone region of inner Sardinia...
May 16, 2017: Aging Clinical and Experimental Research
https://www.readbyqxmd.com/read/28493916/vesicular-glutamate-transporters-play-a-role-in-neuronal-differentiation-of-cultured-svz-derived-neural-precursor-cells
#7
Eduardo H Sánchez-Mendoza, Victor Bellver-Landete, Carmen Arce, Thorsten R Doeppner, Dirk M Hermann, María Jesús Oset-Gasque
The role of glutamate in the regulation of neurogenesis is well-established, but the role of vesicular glutamate transporters (VGLUTs) and excitatory amino acid transporters (EAATs) in controlling adult neurogenesis is unknown. Here we investigated the implication of VGLUTs in the differentiation of subventricular zone (SVZ)-derived neural precursor cells (NPCs). Our results show that NPCs express VGLUT1-3 and EAAT1-3 both at the mRNA and protein level. Their expression increases during differentiation closely associated with the expression of marker genes...
2017: PloS One
https://www.readbyqxmd.com/read/28473660/pegylated-plga-based-phase-shift-nanodroplets-combined-with-focused-ultrasound-for-blood-brain-barrier-opening-in-rats
#8
Xiang Zhang, Jiangang Hu, Guanjian Zhao, Ning Huang, Ying Tan, Li Pi, Qing Huang, Feng Wang, Zhigang Wang, Zhibiao Wang, Yuan Cheng
Previous studies have shown that focused ultrasound (FUS) combined with systematic administration of microbubbles (MBs) can open the blood brain barrier (BBB) locally, transiently and reversibly. However, because of the micro size diameters, MBs are restricted in the intravascular space and cannot extravasate into diseased sites through the opened BBB. In this study, we fabricated one kind of nanoscale droplets which consisted of encapsulated liquid perfluoropentane cores and poly (ethyleneglycol) - poly (lactide-co-glycolic acid) shells...
April 17, 2017: Oncotarget
https://www.readbyqxmd.com/read/28438153/damage-to-the-macula-associated-with-led-derived-blue-laser-exposure-a-case-report
#9
Lingling Liang, Zhihua Cui, Chengwei Lu, Qian Hao, Yajuan Zheng
BACKGROUND: Light emitting diodes laser is emerging as an important source of light replacing conventional lights. It is widely used for illumination in the bar where young people love to go. But not everyone knows about the light damage to the eye especially to the macula. In this article, we report the case of a macular damage induced by LED-derived blue laser in a bar, studied with optical coherence tomography (OCT) to evaluate the retinal lesion and multifocal electroretinography (mfERG) to evaluate functional damage...
April 24, 2017: BMC Ophthalmology
https://www.readbyqxmd.com/read/28427391/fish-with-red-fluorescent-eyes-forage-more-efficiently-under-dim-blue-green-light-conditions
#10
Ulrike Katharina Harant, Nicolaas Karel Michiels
BACKGROUND: Natural red fluorescence is particularly conspicuous in the eyes of some small, benthic, predatory fishes. Fluorescence also increases in relative efficiency with increasing depth, which has generated speculation about its possible function as a "light organ" to detect cryptic organisms under bluish light. Here we investigate whether foraging success is improved under ambient conditions that make red fluorescence stand out more, using the triplefin Tripterygion delaisi as a model system...
April 20, 2017: BMC Ecology
https://www.readbyqxmd.com/read/28403784/synthesis-characterization-of-4-anilino-6-7-dimethoxy-quinazoline-derivatives-as-potential-anti-angiogenic-agents
#11
Saligrama Devegowda Preethi, Balaji Kyathegowdanadoddi Srinivas, Prasanna Doddakunche Shivaramu, Swaroop Toreshettahally Ramesh, Shankar Jayarama, Rangappa KanchKanchugarakoppal Subbegowda, Lokesh Siddalingaiah Gowda
A series of novel 4-anilino-6,7-dimethoxy quinazoline derivatives were synthesized and characterized using 1H, 13C NMR and FT-IR and mass spectroscopic techniques. Cytotoxicity assays were performed for all compounds against different cell lines such as human colon carcinoma (HCT116), human chronic myeloid leukemia (K562) and human breast cancer (SKBR3) cell lines using 3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide (MTT), Tryphan blue and Lactose dehydrogenase release assay. Among all compounds, RB4, RB7 showed moderate activity whereas RB1 showed most potent activity comparable with that of the standard drug cisplatin against all three cells lines...
April 12, 2017: Anti-cancer Agents in Medicinal Chemistry
https://www.readbyqxmd.com/read/28401305/evaluation-of-specific-neural-marker-gap-43-and-th-combined-with-masson-trichrome-staining-for-forensic-autopsy-cases-with-old-myocardial-infarction
#12
Tian-Shui Yu, Xu Wang, Hai-Dong Zhang, Ru-Feng Bai, Rui Zhao, Da-Wei Guan
It has been a puzzling forensic task to determine the cause of death as a result of old myocardial infarction (OMI) in the absence of recognizable acute myocardial infarction. Recent studies indicated that the heterogeneous cardiac nerve sprouting and sympathetic hyperinnervation at border zones of the infarcted site played important roles in sudden cardiac death (SCD). So, the present study explored the value of growth associated protein-43 (GAP-43) and tyrosine hydroxylase (TH) as objective and specific neural biomarkers combined with Masson-trichrome staining for forensic autopsy cases...
April 11, 2017: International Journal of Legal Medicine
https://www.readbyqxmd.com/read/28395776/evaluation-of-coloured-materials-in-microfluidic-flow-cells-for-chemiluminescence-detection
#13
Kara B Spilstead, Richard Alexander, Egan H Doeven, Stephen J Haswell, Neil W Barnett, Paul S Francis
Recent advances in the construction of chemiluminescence flow-cells has included high precision milling of channels into a range of different polymer materials, in efforts to maximise the transfer of light from the chemical reaction to the photodetector. However, little is known of the extent that the colour of polymer materials will influence this transfer. This may become increasingly important as chemiluminescence detection zones are integrated with other operations within microfluidic devices or micro total analysis systems (μTAS)...
May 22, 2017: Analytica Chimica Acta
https://www.readbyqxmd.com/read/28364605/changes-in-soil-characteristics-and-c-dynamics-after-mangrove-clearing-vietnam
#14
Séraphine Grellier, Jean-Louis Janeau, Nhon Dang Hoai, Cuc Nguyen Thi Kim, Quynh Le Thi Phuong, Thao Pham Thi Thu, Nhu-Trang Tran-Thi, Cyril Marchand
Of the blue carbon sinks, mangroves have one of the highest organic matter (OM) storage capacities in their soil due to low mineralization processes resulting from waterlogging. However, mangroves are disappearing worldwide because of demographic increases. In addition to the loss of CO2 fixation, mangrove clearing can strongly affect soil characteristics and C storage. The objectives of the present study were to quantify the evolution of soil quality, carbon stocks and carbon fluxes after mangrove clearing...
March 29, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28359553/planar-yeast-estrogen-screen-with-resorufin-%C3%AE-d-galactopyranoside-as-substrate
#15
Dinah Schick, Wolfgang Schwack
For the planar yeast estrogen screen (pYES), 4-methylumbelliferyl-β-d-galactopyranoside was generally employed as substrate, delivering blue fluorescing 4-methylumbelliferone after enzymatic cleavage by the YES reporter β-d-galactosidase as the positive signal for the presence of estrogen active compounds (EAC). As environmental samples like waste water also contain blue fluorescent components, it is difficult to differentiate them from pYES signals. Therefore, resorufin-β-d-galactopyranoside (RGP), providing the orange fluorescing resorufin after enzymatic cleavage, was introduced as pYES substrate to determine EAC...
May 12, 2017: Journal of Chromatography. A
https://www.readbyqxmd.com/read/28358421/extravasated-platelet-aggregation-in-the-livers-of-rats-with-drug%C3%A2-induced-hepatic-sinusoidal-obstruction-syndrome
#16
Miki Hirata, Hidehiro Tajima, Tomoharu Miyashita, Takashi Miyata, Shinichi Nakanuma, Isamu Makino, Hironori Hayashi, Katsunobu Oyama, Hiroyuki Takamura, Itasu Ninomiya, Sachio Fushida, Hiroki Nakata, Shoichi Iseki, Shinichi Harada, Tomohiko Wakayama, Tetsuo Ohta
Oxaliplatin-based chemotherapy plays an important role in the treatment of colorectal liver metastases. Oxaliplatin, however, causes sinusoidal obstruction syndrome (SOS), which is characterized by portal hypertension, splenomegaly, thrombocytopenia, and liver dysfunction. SOS is diagnosed histopathologically by disruption of the sinusoidal endothelium, collagen deposition, fibrosis especially around zone 3, dilatation of the sinusoidal space and congestion. This study assessed the characteristics of a rat model of SOS...
May 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28358226/excess-maternal-and-postnatal-thyroxine-alters-chondrocyte-numbers-and-the-composition-of-the-extracellular-matrix-of-growth-cartilage-in-rats
#17
Lorena Gabriela Rocha Ribeiro, Juneo Freitas Silva, Natália de Melo Ocarino, Eliane Gonçalves de Melo, Rogéria Serakides
Purpose/Aim: The aim of this study was to evaluate the effects of excess maternal and postnatal thyroxine on chondrocytes and the extracellular matrix (ECM) of growth cartilage. MATERIALS AND METHODS: We used 16 adult female Wistar rats divided into two groups: thyroxine treatment and control. From weaning to 40 days of age, offspring of the treated group (n = 8) received L-thyroxine. Plasma free T4 was measured. Histomorphometric analysis was performed on thyroids and femurs of all offspring...
February 13, 2017: Connective Tissue Research
https://www.readbyqxmd.com/read/28350664/human-adipose-derived-stem-cells-labeled-with-plasmonic-gold-nanostars-for-cellular-tracking-and-photothermal-cancer-cell-ablation
#18
Ronnie L Shammas, Andrew M Fales, Bridget M Crawford, Amy J Wisdom, Gayathri R Devi, David A Brown, Tuan Vo-Dinh, Scott T Hollenbeck
BACKGROUND: Gold nanostars are unique nanoplatforms that can be imaged in real time and transform light energy into heat to ablate cells. Adipose-derived stem cells migrate toward tumor niches in response to chemokines. The ability of adipose-derived stem cells to migrate and integrate into tumors makes them ideal vehicles for the targeted delivery of cancer nanotherapeutics. METHODS: To test the labeling efficiency of gold nanostars, undifferentiated adipose-derived stem cells were incubated with gold nanostars and a commercially available nanoparticle (Qtracker), then imaged using two-photon photoluminescence microscopy...
April 2017: Plastic and Reconstructive Surgery
https://www.readbyqxmd.com/read/28341632/microvascular-leakage-in-acute-myocardial-infarction-characterization-by-histology-biochemistry-and-magnetic-resonance-imaging
#19
Xiao-Ming Gao, Qi-Zhu Wu, Helen Kiriazis, Yidan Su, Li-Ping Han, James Pearson, Andrew Taylor, Xiao-Jun Du
Cardiac microvascular obstruction (MVO) following ischemia/reperfusion (I/R) has been well studied, but microvascular leakage (MVL) remains largely unexplored. We characterized MVL in the mouse I/R model by histology, biochemistry and cardiac magnetic resonance (CMR) imaging. I/R was induced surgically in mice. MVL was determined by administrating microvascular permeability tracer Evans blue (EB) and/or gadolinium-DTPA contrast. The size of MVL, infarction and MVO in the heart was quantified histologically...
March 24, 2017: American Journal of Physiology. Heart and Circulatory Physiology
https://www.readbyqxmd.com/read/28326231/in-situ-monitoring-by-raman-spectroscopy-of-the-thermal-doping-of-graphene-and-mos2-in-o2-controlled-atmosphere
#20
Aurora Piazza, Filippo Giannazzo, Gianpiero Buscarino, Gabriele Fisichella, Antonino La Magna, Fabrizio Roccaforte, Marco Cannas, Franco Mario Gelardi, Simonpietro Agnello
The effects of temperature and atmosphere (air and O2) on the doping of monolayers of graphene (Gr) on SiO2 and Si substrates, and on the doping of MoS2 multilayer flakes transferred on the same substrates have been investigated. The investigations were carried out by in situ micro-Raman spectroscopy during thermal treatments up to 430 °C, and by atomic force microscopy (AFM). The spectral positions of the G and 2D Raman bands of Gr undergo only minor changes during treatment, while their amplitude and full width at half maximum (FWHM) vary as a function of the temperature and the used atmosphere...
2017: Beilstein Journal of Nanotechnology
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