keyword
https://read.qxmd.com/read/21909364/transcription-of-dwarf4-plays-a-crucial-role-in-auxin-regulated-root-elongation-in-addition-to-brassinosteroid-homeostasis-in-arabidopsis-thaliana
#21
JOURNAL ARTICLE
Yuya Yoshimitsu, Kiwamu Tanaka, Wataru Fukuda, Tadao Asami, Shigeo Yoshida, Ken-Ichiro Hayashi, Yuji Kamiya, Yusuke Jikumaru, Tomoaki Shigeta, Yasushi Nakamura, Tomoaki Matsuo, Shigehisa Okamoto
The expression of DWARF4 (DWF4), which encodes a C-22 hydroxylase, is crucial for brassinosteroid (BR) biosynthesis and for the feedback control of endogenous BR levels. To advance our knowledge of BRs, we examined the effects of different plant hormones on DWF4 transcription in Arabidopsis thaliana. Semi-quantitative reverse-transcriptase PCR showed that the amount of the DWF4 mRNA precursor either decreased or increased, similarly with its mature form, in response to an exogenously applied bioactive BR, brassinolide (BL), and a BR biosynthesis inhibitor, brassinazole (Brz), respectively...
2011: PloS One
https://read.qxmd.com/read/21901252/soluble-mediators-released-from-pi-ibs-patients-colon-induced-alteration-of-mast-cell-involvement-of-reactive-oxygen-species
#22
JOURNAL ARTICLE
Wei Han, Xuefeng Lu, Xiaoqing Jia, Tao Zhou, Chenghao Guo
BACKGROUND: Growing evidence suggests that patients with post-infectious irritable bowel syndrome (PI-IBS) have increased mast cell activation, and that mucosal soluble mediators are involved in the pathophysiology of visceral hyperalgesia. In addition, previous findings show that reactive oxygen species (ROS) and protease-activated receptors (PARs) are mediators of persistent hyperalgesia. AIMS: This article aims to investigate: (1) the ability of soluble factors from colonic biopsies to active peritoneal mast cells (PMCs) in vitro; (2) whether the effects of PMCs degranulation induced by soluble mediators are related to PARs activation; and (3) the ability of phenyl N-tert-butylnitrone (PBN), a ROS scavenger, to modify these alterations...
February 2012: Digestive Diseases and Sciences
https://read.qxmd.com/read/21543766/kr%C3%A3-ppel-like-factor-9-loss-of-expression-in-human-endometrial-carcinoma-links-altered-expression-of-growth-regulatory-genes-with-aberrant-proliferative-response-to-estrogen
#23
JOURNAL ARTICLE
Christian D Simmons, John Mark P Pabona, Melissa E Heard, Theodore M Friedman, Michael T Spataro, Amy L Godley, Frank A Simmen, Alexander F Burnett, Rosalia C M Simmen
Endometrial cancer is the most commonly diagnosed female genital tract malignancy. Krüppel-like factor 9 (KLF9), a member of the evolutionarily conserved Sp family of transcription factors, is expressed in uterine stroma and glandular epithelium, where it affects cellular proliferation, differentiation, and apoptosis. Deregulated expression of a number of Sp proteins has been associated with multiple types of human tumors, but a role for KLF9 in endometrial cancer development and/or progression is unknown...
August 2011: Biology of Reproduction
https://read.qxmd.com/read/20932822/the-cap-n-collar-transcription-factor-nrf2-mediates-both-intrinsic-resistance-to-environmental-stressors-and-an-adaptive-response-elicited-by-chemopreventive-agents-that-determines-susceptibility-to-electrophilic-xenobiotics
#24
JOURNAL ARTICLE
Larry G Higgins, John D Hayes
Transcription factor Nrf2 regulates genes encoding drug-metabolising enzymes and drug transporters, as well as enzymes involved in the glutathione, thioredoxin and peroxiredoxin antioxidant pathways. Using mouse embryonic fibroblast (MEF) cells from Nrf2(+/+) and Nrf2(-/-) mice, in conjunction with the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) cytotoxicity assay, we have shown that loss of Nrf2 diminishes the intrinsic resistance of mutant fibroblasts towards isothiocyanates (i.e. sulforaphane), epoxides (i...
June 30, 2011: Chemico-biological Interactions
https://read.qxmd.com/read/19815709/activated-camp-response-element-binding-protein-is-overexpressed-in-human-mesotheliomas-and-inhibits-apoptosis
#25
JOURNAL ARTICLE
Arti Shukla, Marcus W Bosenberg, Maximilian B MacPherson, Kelly J Butnor, Nicholas H Heintz, Harvey I Pass, Michele Carbone, Joseph R Testa, Brooke T Mossman
Little is known about the cellular mechanisms contributing to the development and chemoresistance of malignant mesothelioma (MM), an aggressive asbestos-associated tumor. A human mesothelial cell line (LP9/TERT-1) and isolated human pleural mesothelial cells showed rapid and protracted asbestos-induced cAMP response element binding protein (CREB1) phosphorylation, which was inhibited in LP9/TERT-1 cells by small molecule inhibitors of epidermal growth factor receptor phosphorylation and protein kinase A. Asbestos increased expression of several CREB target genes (c-FOS, EGR-1, MKP1, BCL2, and MMP13) and apoptosis, which was enhanced using small interfering CREB...
November 2009: American Journal of Pathology
https://read.qxmd.com/read/19183254/curcumin-quercetin-and-tbhq-modulate-glutathione-levels-in-astrocytes-and-neurons-importance-of-the-glutamate-cysteine-ligase-modifier-subunit
#26
JOURNAL ARTICLE
Suzie Lavoie, Ying Chen, Timothy P Dalton, René Gysin, Michel Cuénod, Pascal Steullet, Kim Q Do
A decrease in GSH levels, the main redox regulator, can be observed in neurodegenerative diseases as well as in schizophrenia. In search for substances able to increase GSH, we evaluated the ability of curcumin (polyphenol), quercetin (flavonoid), and tert-butylhydroquinone (tBHQ) to up-regulate GSH-synthesizing enzymes. The gene expression, activity, and product levels of these enzymes were measured in cultured neurons and astrocytes. In astrocytes, all substances increased GSH levels and the activity of the rate-limiting synthesizing enzyme, glutamate cysteine ligase (GCL)...
March 2009: Journal of Neurochemistry
https://read.qxmd.com/read/17200665/stimulation-of-apical-and-basolateral-vegf-a-and-vegf-c-secretion-by-oxidative-stress-in-polarized-retinal-pigment-epithelial-cells
#27
JOURNAL ARTICLE
Ram Kannan, Ning Zhang, Parameswaran G Sreekumar, Christine K Spee, Anthony Rodriguez, Ernesto Barron, David R Hinton
PURPOSE: To investigate whether oxidative stress modulates vascular endothelial growth factor (VEGF)-A and VEGF-C expression and polarized secretion in a human retinal pigment epithelium cell line (ARPE-19). METHODS: Long-term culture of ARPE-19 cells in Dulbecco's modified Eagle medium (DMEM)/F12 containing 1% fetal bovine serum (FBS) on transwell filters (12 mm or 6 mm, pore size 0.4 microm) was performed to produce polarized retinal pigment epithelium (RPE) monolayers...
2006: Molecular Vision
https://read.qxmd.com/read/17192422/okl38-is-an-oxidative-stress-response-gene-stimulated-by-oxidized-phospholipids
#28
JOURNAL ARTICLE
Rongsong Li, Wendy Chen, Rolando Yanes, Sangderk Lee, Judith A Berliner
Oxidized 1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphorylcholine (OxPAPC) is present in oxidative modified LDL and accumulates in lesions of many chronic inflammatory diseases, such as atherosclerosis. In a microarray study, OxPAPC has been demonstrated to modulate the expression of >700 genes in human aortic endothelial cells. We found that the levels of mRNA for OKL38 [also named Bone marrow Derived Growth Factor (BDGI)], a tumor growth inhibitor, were strongly increased by OxPAPC. Here, we report that OKL38 is regulated by an oxidative signal induced by OxPAPC and its component lipid 1-palmitoyl-2-epoxyisoprostane E2-sn-glycero-3-phosphorylcholine...
March 2007: Journal of Lipid Research
https://read.qxmd.com/read/16536490/an-efficient-convenient-solid-phase-synthesis-of-amino-acid-modified-peptide-nucleic-acid-monomers-and-oligomers
#29
JOURNAL ARTICLE
Baghavathy S Balaji, Fabio Gallazzi, Fang Jia, Michael R Lewis
An efficient and highly versatile method for the synthesis of amino acid-modified peptide nucleic acid (PNA) monomers is described. By using solid-phase Fmoc techniques, such monomers can be assembled readily in a stepwise manner and obtained in high yield with minimal purification. Protected neutral hydrophilic, acidic, and basic amino acids were coupled to 2-chlorotrityl chloride resin. Following Fmoc removal, innovative conditions for the key step, reductive alkylation with N-Fmoc-aminoacetaldehyde, were developed to circumvent problems encountered with previously reported methods...
March 2006: Bioconjugate Chemistry
https://read.qxmd.com/read/10393048/regulation-of-gamma-glutamylcysteine-synthetase-subunit-gene-expression-in-retinal-m%C3%A3-ller-cells-by-oxidative-stress
#30
JOURNAL ARTICLE
S C Lu, Y Bao, Z Z Huang, V P Sarthy, R Kannan
PURPOSE: To study regulation of gamma-glutamylcysteine synthetase (GCS) heavy and light subunit gene expression in Müller cells under conditions of oxidative stress. METHODS: Experiments were carried out with an SV40 transformed cell line (rMC-1) that exhibits the phenotype of rat retinal Müller cells. Endogenous glutathione levels were modified by treating cells with diethyl maleate (DEM), D,L-buthionine sulfoximine (BSO), or tert-butylhydroquinone (TBH). In other experiments, cells were grown in either high (28 mM) or normal (5...
July 1999: Investigative Ophthalmology & Visual Science
https://read.qxmd.com/read/10098886/induction-of-glucose-6-phosphate-dehydrogenase-by-lipopolysaccharide-contributes-to-preventing-nitric-oxide-mediated-glutathione-depletion-in-cultured-rat-astrocytes
#31
JOURNAL ARTICLE
P García-Nogales, A Almeida, E Fernández, J M Medina, J P Bolaños
Treatment of cultured rat astrocytes with lipopolysaccharide (LPS; 1 microg/ml) increased mRNA expression of glucose-6-phosphate dehydrogenase (G6PD), the rate-limiting step in the pentose phosphate pathway (PPP), in a time-dependent fashion (0-24 h). This effect was accompanied by an increase in G6PD activity (1.74-fold) and in the rate of glucose oxidation through the PPP (6.32-fold). Inhibition of inducible nitric oxide synthase (iNOS) activity by 2-amino-5,6-dihydro-6-methyl-4H-1,3-thiazine (AMT; 50 microM) did not alter the LPS-mediated enhancement of G6PD mRNA expression or PPP activity...
April 1999: Journal of Neurochemistry
https://read.qxmd.com/read/8830930/psk-protects-macrophages-from-lipoperoxide-accumulation-and-foam-cell-formation-caused-by-oxidatively-modified-low-density-lipoprotein
#32
COMPARATIVE STUDY
C Yuan, Z Mei, S Liu, L Yi
In previous works, it has been evidenced that lipoperoxidative injury to macrophages caused by oxidatively modified low-density lipoprotein (O-LDL) plays an important role in foam cell formation, and that PSK, a protein bound polysaccharide extracted from the class Basidiomycetes Coriolus Versicolor, can protect macrophages from lipoperoxidative injury induced by tert-butyl hydroperoxide (tbOOH). In this paper PSK protection of macrophages from lipoperoxide (LPO) accumulation and foam cell formation caused by O-LDL and its action mechanism were further studied...
August 2, 1996: Atherosclerosis
https://read.qxmd.com/read/8631767/apoptosis-induced-by-transforming-growth-factor-beta-in-fetal-hepatocyte-primary-cultures-involvement-of-reactive-oxygen-intermediates
#33
JOURNAL ARTICLE
A Sánchez, A M Alvarez, M Benito, I Fabregat
Transforming growth factor-beta (TGF-beta), a growth regulator of fetal hepatocytes in primary culture, also regulates death of these cells. Dose-response analysis showed that the TGF-beta concentration needed to induce hepatocyte death (2.5 ng/ml) was 5 times that needed to inhibit growth in these cells (0.5 ng/ml). In response to TGF-beta, hepatocytes induced DNA fragmentation and the appearance of nuclei with a DNA content lower than 2C (diploid content), typical of a programmed cell death model. TGF-beta-induced apoptosis in fetal hepatocytes was preceded by an induction of reactive oxygen species production and a decrease in the glutathione intracellular content, indicating that this factor induces oxidative stress in fetal hepatocytes...
March 29, 1996: Journal of Biological Chemistry
https://read.qxmd.com/read/1472074/effects-of-altered-calcium-homeostasis-on-the-expression-of-glutathione-s-transferase-isozymes-in-primary-cultured-rat-hepatocytes
#34
JOURNAL ARTICLE
R S Dwivedi, A Gruebele, R F Novak
The effects of altered Ca2+ homeostasis on glutathione S-transferase (GST) isozyme expression in cultured primary rat hepatocytes were examined. Isolated hepatocytes were cultured on Vitrogen substratum in serum-free modified Chee's essential medium and treated with Ca2+ ionophore A23187 at 120 hr post-plating. GST activity increased slightly, albeit significantly, in a concentration-dependent manner in A23187-treated hepatocytes relative to untreated controls. Western blot analysis using GST class alpha and mu specific antibodies showed an approximately 1...
December 1, 1992: Biochemical Pharmacology
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