Yuya Yoshimitsu, Kiwamu Tanaka, Wataru Fukuda, Tadao Asami, Shigeo Yoshida, Ken-Ichiro Hayashi, Yuji Kamiya, Yusuke Jikumaru, Tomoaki Shigeta, Yasushi Nakamura, Tomoaki Matsuo, Shigehisa Okamoto
The expression of DWARF4 (DWF4), which encodes a C-22 hydroxylase, is crucial for brassinosteroid (BR) biosynthesis and for the feedback control of endogenous BR levels. To advance our knowledge of BRs, we examined the effects of different plant hormones on DWF4 transcription in Arabidopsis thaliana. Semi-quantitative reverse-transcriptase PCR showed that the amount of the DWF4 mRNA precursor either decreased or increased, similarly with its mature form, in response to an exogenously applied bioactive BR, brassinolide (BL), and a BR biosynthesis inhibitor, brassinazole (Brz), respectively...
2011: PloS One