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https://www.readbyqxmd.com/read/27911382/a-simple-mechanical-procedure-to-create-limbal-stem-cell-deficiency-in-mouse
#1
Neda Afsharkhamseh, Elham Ghahari, Medi Eslani, Ali R Djalilian
Limbal stem cell deficiency (LSCD) is a state of malfunction or loss of limbal epithelial stem cells, after which the corneal epithelium is replaced with conjunctiva. Patients suffer from recurrent corneal defects, pain, inflammation, and loss of vision. Previously, a murine model of LSCD was described and compared to two other models. The goal was to produce a consistent mouse model of LSCD that both mimics the phenotype in humans and lasts long enough to make it possible to study the disease pathophysiology and to evaluate new treatments...
November 17, 2016: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/27890620/drug-induced-corneal-epithelial-changes
#2
REVIEW
Michael B Raizman, Pedram Hamrah, Edward J Holland, Terry Kim, Francis S Mah, Christopher J Rapuano, Roger G Ulrich
Drugs across many pharmacologic classes induce corneal epithelial changes. Many of these drugs have cationic amphiphilic structures, with a hydrophobic ring and hydrophilic cationic amine side chain that allow them to cross cell membranes. These drugs lead to intracellular phospholipid accumulation, often manifested in the cornea by vortex keratopathy, with no effect on visual acuity and few ocular symptoms. Other drugs, notably antineoplastic agents, produce a fine diffuse corneal haze, sometimes accompanied by decreased vision that can be dose limiting...
November 24, 2016: Survey of Ophthalmology
https://www.readbyqxmd.com/read/27811562/outcomes-of-descemet-stripping-automated-endothelial-keratoplasty-in-toxic-anterior-segment-syndrome-after-phacoemulsification
#3
Manpreet Kaur, Jeewan S Titiyal, Ruchita Falera, Tarun Arora, Namrata Sharma
PURPOSE: To evaluate the visual and anatomical outcomes of Descemet stripping automated endothelial keratoplasty (DSAEK) in patients with toxic anterior segment syndrome (TASS). METHODS: Fourteen eyes of 14 patients who underwent clear corneal DSAEK for endothelial decompensation secondary to TASS after phacoemulsification were included in this prospective study. Intraoperative surgical difficulties were noted. Preoperative and postoperative evaluation of visual acuity, slit-lamp examination, intraocular pressure, central corneal thickness, and endothelial count was performed in all cases...
November 2, 2016: Cornea
https://www.readbyqxmd.com/read/27804048/clinical-evaluation-of-two-types-of-intracorneal-ring-segments-icrs-for-keratoconus
#4
Waleed S Al-Tuwairqi, Uchechukwu L Osuagwu, Haya Razzouk, Ahmed AlHarbi, Kelechi C Ogbuehi
PURPOSE: To compare the outcome measures following implantation of two types of ICRS. METHODS: Forty-four eyes of keratoconic patients (aged 18-50 years) were randomly assigned to femtosecond laser-assisted Keraring ICRS (Group A) or MyoRing (Group B) implantation. Uncorrected (UDVA) and corrected (CDVA) distance visual acuities, refraction, keratometry (K), and aberrations were compared preoperatively, at 3 and 6 months postoperatively. A survey was conducted to assess patient's satisfaction, 6 months postoperatively...
November 1, 2016: International Ophthalmology
https://www.readbyqxmd.com/read/27795856/polar-value-analysis-of-corneal-astigmatism-in-intrastromal-corneal-ring-segment-implantation
#5
Chang Rae Rho, Min-Ji Kim, Choun-Ki Joo
Purpose. To evaluate surgically induced astigmatism (SIA) and the average corneal power change in symmetric intrastromal corneal ring segment (ICRS) implantation. Methods. The study included 34 eyes of 34 keratoconus patients who underwent symmetric Intacs SK ICRS implantation. The corneal pocket incision meridian was the preoperative steep meridian. Corneal power data were obtained before and 3 months after Intacs SK ICRS implantation using scanning-slit topography. Polar value analysis was used to evaluate the SIA...
2016: Journal of Ophthalmology
https://www.readbyqxmd.com/read/27766813/familial-lecithin-cholesterol-acyl-transferase-deficiency-with-chronic-kidney-disease
#6
N Gopalakrishnan, R Arul, J Dhanapriya, T Dinesh Kumar, R Sakthirajan, T Balasubramaniyan
Familial lecithin-cholesterol acyltransferase (LCAT) deficiency is a rare autosomal recessive (AR) disease caused by mutation in the LCAT gene. LCAT enzyme esterifies cholesterol molecules in high-density lipoprotein(HDL) and low density-lipoprotein (LDL) particles. This enzyme deficiency is characterised by progressive corneal opacification, glomerulopathy, mild - moderate haemolytic anaemia and very low plasma levels of HDL. We here report a 34 year-old lady who presented with hypertension, nephrotic proteinuria, renal failure, corneal ring opacities, anemia and dyslipidemia...
October 2016: Journal of the Association of Physicians of India
https://www.readbyqxmd.com/read/27742843/a-human-corneal-epithelial-cell-line-model-for-limbal-stem-cell-biology-and-limbal-immunobiology
#7
Bakiah Shaharuddin, Sajjad Ahmad, Nani Md Latar, Simi Ali, Annette Meeson
: : Limbal stem cell (LSC) deficiency is a visually debilitating condition caused by abnormal maintenance of LSCs. It is treated by transplantation of donor-derived limbal epithelial cells (LECs), the success of which depends on the presence and quality of LSCs within the transplant. Understanding the immunobiological responses of these cells within the transplants could improve cell engraftment and survival. However, human corneal rings used as a source of LSCs are not always readily available for research purposes...
October 14, 2016: Stem Cells Translational Medicine
https://www.readbyqxmd.com/read/27721784/corneal-ring-infiltrates-caused-by-serratia-marcescens-in-a-patient-with-human-immunodeficiency-virus
#8
Winai Chaidaroon, Sumet Supalaset
PURPOSE: To describe corneal ring infiltrates caused by Serratia marcescens in a patient with human immunodeficiency virus (HIV-1) who wore contact lenses. METHODS: A case study of a patient with keratitis due to an infection caused by S. marcescens and exhibiting corneal ring infiltrates was reviewed for history, clinical manifestation, microscopic study, and management. RESULTS: A 29-year-old man who had a history of contact lens wear and HIV-1 infection was admitted to hospital because of blurred vision, redness, and corneal infiltrates in the shape of a ring in the left eye...
May 2016: Case Reports in Ophthalmology
https://www.readbyqxmd.com/read/27678470/ring-shaped-dysphotopsia-associated-with-posterior-chamber-phakic-implantable-collamer-lenses-with-a-central-hole
#9
Youngsub Eom, Dae Wook Kim, Dongok Ryu, Jun-Heon Kim, Seul Ki Yang, Jong Suk Song, Sug-Whan Kim, Hyo Myung Kim
PURPOSE: To evaluate the incidence of central hole-induced ring-shaped dysphotopsia after posterior chamber phakic implantable collamer lens (ICL) with central hole (hole ICL) implantation and to investigate the causes of central hole-induced dysphotopsia. METHODS: The clinical study enrolled 29 eyes of 15 consecutive myopic patients implanted with hole ICL. The incidence of ring-shaped dysphotopsia after hole ICL implantation was evaluated. In the experimental simulation study, non-sequential ray tracing was used to construct myopic human eye models with hole ICL and ICL without a central hole (conventional ICL)...
September 28, 2016: Acta Ophthalmologica
https://www.readbyqxmd.com/read/27672592/evaluation-of-anterior-and-posterior-surfaces-of-the-cornea-using-a-dual-scheimpflug-analyzer-in-keratoconus-patients-implanted-with-intrastromal-corneal-ring-segments
#10
Leonardo Torquetti, Carlos Arce, Jesús Merayo-Lloves, Guilherme Ferrara, Paulo Ferrara, Brenno Signorelli, Armando Signorelli
AIM: To evaluate corneal parameters measured with a dual Scheimpflug analyzer in keratoconus patients implanted with intrastromal corneal ring segments (ICRS). METHODS: Fifty eyes of 40 keratoconus patients had Ferrara ICRS implantation from November 2010 to April 2014. Uncorrected visual acuity (UCVA), best corrected visual acuity (BCVA), refraction, keratometry, asphericity, elevation, pachymetry, root mean square (RMS), spherical aberration and coma were studied...
2016: International Journal of Ophthalmology
https://www.readbyqxmd.com/read/27661992/measuring-intraocular-pressure-in-patients-with-keratoconus-with-and-without-intrastromal-corneal-ring-segments
#11
Carmen Mendez-Hernandez, Paula Arribas-Pardo, Ricardo Cuiña-Sardiña, Cristina Fernandez-Perez, Rosalia Mendez-Fernandez, Federico Saenz-Frances, Jose M Benitez-Del-Castillo, Julian Garcia-Feijoo
PURPOSE: To compare intraocular pressure (IOP) measurements made using 5 tonometers in keratoconic eyes with and without intrastromal corneal ring segments. METHODS AND PATIENTS: This was an observational case series study. A total of 147 eyes of 147 patients with keratoconus, 74 of which had undergone corneal ring segment placement, were prospectively evaluated. IOP was measured using the tonometers Tonopen XL, Pascal dynamic contour tonometer, iCare Pro, ocular response analyzer (ORA), and Goldmann applanation (GAT) in random order...
September 21, 2016: Journal of Glaucoma
https://www.readbyqxmd.com/read/27639912/-ecchymosis-as-the-presenting-manifestation-of-wilson-disease-a-case-report
#12
G Bayer, A Bauvois, J Mankikian, M Tardieu, F Maillot, E Salame, F Woimant, A Poujois, M Viana, A Legras
INTRODUCTION: The presence of a psychiatric disorder during the course of an organic disease is a common cause of delayed diagnosis. CASE REPORT: We report a 16-year-old girl who was admitted with thrombocytopenia and had a two-year history of neuropsychiatric disorder which was attributed to a difficult family situation. Neurological examination showed a frontal lobe disorder and extrapyramidal manifestations. The thrombocytopenia was attributed to liver cirrhosis...
September 14, 2016: La Revue de Médecine Interne
https://www.readbyqxmd.com/read/27621780/mini-scleral-contact-lens-for-management-of-poor-visual-outcomes-after-intrastromal-corneal-ring-segments-implantation-in-keratoconus
#13
Fatemeh Alipour, Firoozeh Rahimi, Mohammad Naser Hashemian, Zahra Ajdarkosh, Ramak Roohipoor, Masoumeh Mohebi
PURPOSE: To evaluate the feasibility and efficacy of mini-scleral design (MSD) contact lenses to treat keratoconus patients who were unsatisfied with the results of corneal inlay. METHODS: In this prospective interventional case series, 9 eyes of 6 keratoconus patients who were unsatisfied with the results of corneal inlay were fitted with MSD contact lenses. Demographic data, uncorrected visual acuity (UCVA), best spectacle-corrected visual acuity (BSCVA), and higher order aberrations (HOAs) were evaluated before contact lens fitting...
July 2016: Journal of Ophthalmic & Vision Research
https://www.readbyqxmd.com/read/27595913/the-effect-of-air-sf6-and-c3f8-on-immortalized-human-corneal-endothelial-cells
#14
Marina Hesse, David Kuerten, Peter Walter, Niklas Plange, Sandra Johnen, Matthias Fuest
PURPOSE: While anterior chamber air bubbles aid attachment during posterior lamellar surgery only for few days, these periods can be prolonged with gases in non-expanding concentrations. To test the effects of different gas compositions on immortalized human corneal endothelial cells (HCEC-12), we utilized Transwell inserts with semipermeable membranes as an artificial anterior chamber model. METHODS: Human corneal endothelial cells (HCEC-12) were cultured on Transwell inserts for 24 hr, then flipped, burdened and sunk with titanium rings in medium (M1), as well as filled with 2 ml of air (A), 20% sulphur hexafluoride (SF6) (S), or 12% C3F8 (C)...
September 6, 2016: Acta Ophthalmologica
https://www.readbyqxmd.com/read/27531296/topography-of-the-corneal-epithelium-and-bowman-layer-in-low-to-moderately-myopic-eyes
#15
Imene Salah-Mabed, Alain Saad, Damien Gatinel
PURPOSE: To compare the epithelium (air-tear film) and Bowman layer's specular topographies in patients having low to moderate myopia corrected by photorefractive keratectomy (PRK). SETTING: Rothschild Foundation, Paris, France. DESIGN: Prospective interventional case series. METHODS: Anterior corneal specular Placido topography using the OPD-Scan II Placido-based topographer was performed in eyes of patients having PRK for myopia before and after epithelial removal...
August 2016: Journal of Cataract and Refractive Surgery
https://www.readbyqxmd.com/read/27505314/pediatric-keratoconus-in-a-tertiary-referral-center-incidence-presentation-risk-factors-and-treatment
#16
Sylvain El-Khoury, Youssef Abdelmassih, Adib Hamade, Elise Slim, Carole G Cherfan, Elias Chelala, Jamal Bleik, Elias F Jarade
PURPOSE: To report the incidence, clinical presentation, risk factors, and treatment outcome of pediatric keratoconus in a tertiary referral eye hospital in Beirut, Lebanon. METHODS: In this retrospective study, the authors evaluated all patients with keratoconus 14 years or younger newly diagnosed at the Beirut Eye Specialist Hospital, Beirut, Lebanon, between January 2010 and December 2014. The incidence of pediatric keratoconus among all pediatric patients and among patients with keratoconus of all ages was assessed...
August 1, 2016: Journal of Refractive Surgery
https://www.readbyqxmd.com/read/27490048/intrastromal-corneal-ring-segment-implantation-in-409-paracentral-keratoconic-eyes
#17
Luis Fernández-Vega Cueto, Carlos Lisa, Aranzazu Poo-López, David Madrid-Costa, Jesús Merayo-Lloves, Jose F Alfonso
PURPOSE: To assess the efficacy outcomes of implanting intrastromal corneal ring segments (ICRS) to correct paracentral keratoconus. METHODS: Four hundred nine eyes with paracentral keratoconus, in which the differences between the axes of refractive cylinder and the corneal flattest meridian and the comatic aberration map were less than 30 degrees, were evaluated before and after the implantation of Ferrara-type ICRS. Snellen uncorrected and best-corrected distance visual acuity, residual refractive errors analyzed with vector analysis, and the root mean square for corneal coma-like aberration for a pupil size of 4...
November 2016: Cornea
https://www.readbyqxmd.com/read/27471196/development-of-a-novel-ex-vivo-equine-corneal-model
#18
Todd L Marlo, Elizabeth A Giuliano, Ajay Sharma, Rajiv R Mohan
OBJECTIVE: To develop an ex vivo equine corneal organ culture model. Specifically, to assess the equine cornea's extracellular matrix and cellularity after 7 days using two different culture techniques: either (i) immersion system or (ii) air/liquid interface system, to determine the best ex vivo equine corneal model. ANIMALS STUDIED: Fourteen healthy equine corneas of various breeds. PROCEDURES: Equine corneas with 2 mm of perilimbal sclera were freshly harvested from 7 horses undergoing humane euthanasia...
July 29, 2016: Veterinary Ophthalmology
https://www.readbyqxmd.com/read/27463630/the-effects-of-riboflavin-and-ultraviolet-light-on-keratocytes-cultured-in-vitro
#19
Joyce L Covre, Priscila C Cristovam, Renata R Loureiro, Rossen M Hazarbassanov, Mauro Campos, Élcio H Sato, José Álvaro P Gomes
PURPOSE: To culture quiescent human keratocytes and evaluate the effects of ultraviolet light and riboflavin on human corneal keratocytes in vitro. METHODS: Keratocytes were obtained from remaining corneoscleral ring donor corneas previously used in corneal transplant surgeries and cultured in DMEM/F12 with 2% FBS until confluence. Characterization of cultured cells was performed by immunofluorescence analysis for anti-cytokeratin-3, anti-Thy-1, anti-α-smooth muscle actin, and anti-lumican...
May 2016: Arquivos Brasileiros de Oftalmologia
https://www.readbyqxmd.com/read/27450910/signs-and-symptoms-of-dry-eye-in-keratoconus-patients-before-and-after-intrastromal-corneal-rings-surgery
#20
Gonzalo Carracedo, Alberto Recchioni, Nicolás Alejandre-Alba, Alba Martin-Gil, Laura Batres, Ignacio Jimenez-Alfaro Morote, Jesús Pintor
PURPOSE: Based on the relationship between keratoconus and dry eye, the aim of this study was to evaluate changes in signs and symptoms of dry eye in keratoconus patients before and after intrastromal corneal ring surgery. METHODS: Fifteen keratoconus patients were enrolled in Fundación Jiménez-Díaz of Madrid and University Clinic of Optometry of the Universidad Complutense de Madrid (Madrid, Spain). Tear break up time (TBUT), Schirmer test without anesthesia, corneal staining, diadenosine tetraphosphate (Ap4A) concentration, and ocular surface disease index (OSDI) were evaluated...
July 22, 2016: Current Eye Research
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