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C.elegans embryo

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https://www.readbyqxmd.com/read/27881437/casein-kinase-ii-is-required-for-proper-cell-division-and-acts-as-a-negative-regulator-of-centrosome-duplication-in-c-elegans-embryos
#1
Jeffrey C Medley, Megan M Kabara, Michael D Stubenvoll, Lauren E DeMeyer, Mi Hye Song
Centrosomes are the primary microtubule-organizing centers that orchestrate microtubule dynamics during the cell cycle. The correct number of centrosomes is pivotal for establishing bipolar mitotic spindles that ensure accurate segregation of chromosomes. Thus, centrioles must duplicate once per cell cycle, one daughter per mother centriole, the process of which requires highly coordinated actions among core factors and modulators. Protein phosphorylation is shown to regulate the stability, localization and activity of centrosome proteins...
November 23, 2016: Biology Open
https://www.readbyqxmd.com/read/27835015/setting-the-clock-for-fail-safe-early-embryogenesis
#2
Rolf Fickentscher, Philipp Struntz, Matthias Weiss
The embryogenesis of the small nematode Caenorhabditis elegans is a remarkably robust self-organization phenomenon. Cell migration trajectories in the early embryo, for example, are well explained by mechanical cues that push cells into positions where they experience the least repulsive forces. Yet, how this mechanically guided progress in development is properly timed has remained elusive so far. Here, we show that cell volumes and division times are strongly anticorrelated during the early embryogenesis of C...
October 28, 2016: Physical Review Letters
https://www.readbyqxmd.com/read/27831827/mitotic-entry-the-interplay-between-cdk1-plk1-and-bora
#3
Alfonso Parrilla, Luca Cirillo, Yann Thomas, Monica Gotta, Lionel Pintard, Anna Santamaria
Polo-like kinase 1 (Plk1) is an important mitotic kinase that is crucial for entry into mitosis after recovery from DNA damage-induced cell cycle arrest. Plk1 activation is promoted by the conserved protein Bora (SPAT-1 in C. elegans), which stimulates the phosphorylation of a conserved residue in the activation loop by the Aurora A kinase. In a recent article published in Cell Reports, we show that the master mitotic kinase Cdk1 contributes to Plk1 activation through SPAT-1/Bora phosphorylation. We identified 3 conserved Sp/Tp residues that are located in the N-terminal, most conserved part, of SPAT-1/Bora...
November 10, 2016: Cell Cycle
https://www.readbyqxmd.com/read/27797074/analysis-of-meiotic-sister-chromatid-cohesion-in-caenorhabditis-elegans
#4
Aaron F Severson
In sexually reproducing organisms, the formation of healthy gametes (sperm and eggs) requires the proper establishment and release of meiotic sister chromatid cohesion (SCC). SCC tethers replicated sisters from their formation in premeiotic S phase until the stepwise removal of cohesion in anaphase of meiosis I and II allows the separation of homologs and then sisters. Defects in the establishment or release of meiotic cohesion cause chromosome segregation errors that lead to the formation of aneuploid gametes and inviable embryos...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/27791108/extracellular-rna-is-transported-from-one-generation-to-the-next-in-caenorhabditis-elegans
#5
Julia Marré, Edward C Traver, Antony M Jose
Experiences during the lifetime of an animal have been proposed to have consequences for subsequent generations. Although it is unclear how such intergenerational transfer of information occurs, RNAs found extracellularly in animals are candidate molecules that can transfer gene-specific regulatory information from one generation to the next because they can enter cells and regulate gene expression. In support of this idea, when double-stranded RNA (dsRNA) is introduced into some animals, the dsRNA can silence genes of matching sequence and the silencing can persist in progeny...
November 1, 2016: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/27791097/stable-caenorhabditis-elegans-chromatin-domains-separate-broadly-expressed-and-developmentally-regulated-genes
#6
Kenneth J Evans, Ni Huang, Przemyslaw Stempor, Michael A Chesney, Thomas A Down, Julie Ahringer
Eukaryotic genomes are organized into domains of differing structure and activity. There is evidence that the domain organization of the genome regulates its activity, yet our understanding of domain properties and the factors that influence their formation is poor. Here, we use chromatin state analyses in early embryos and third-larval stage (L3) animals to investigate genome domain organization and its regulation in Caenorhabditis elegans At both stages we find that the genome is organized into extended chromatin domains of high or low gene activity defined by different subsets of states, and enriched for H3K36me3 or H3K27me3, respectively...
October 25, 2016: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/27790724/control-of-developmental-networks-by-rac-rho-small-gtpases-how-cytoskeletal-changes-during-embryogenesis-are-orchestrated
#7
Beatriz Sáenz-Narciso, Eva Gómez-Orte, Angelina Zheleva, Irene Gastaca, Juan Cabello
Small GTPases in the Rho family act as major nodes with functions beyond cytoskeletal rearrangements shaping the Caenorhabditis elegans embryo during development. These small GTPases are key signal transducers that integrate diverse developmental signals to produce a coordinated response in the cell. In C. elegans, the best studied members of these highly conserved Rho family small GTPases, RHO-1/RhoA, CED-10/Rac, and CDC-42, are crucial in several cellular processes dealing with cytoskeletal reorganization...
October 28, 2016: BioEssays: News and Reviews in Molecular, Cellular and Developmental Biology
https://www.readbyqxmd.com/read/27760363/the-mitotic-spindle-in-the-one-cell-c-elegans-embryo-is-positioned-with-high-precision-and-stability
#8
Jacques Pécréaux, Stefanie Redemann, Zahraa Alayan, Benjamin Mercat, Sylvain Pastezeur, Carlos Garzon-Coral, Anthony A Hyman, Jonathon Howard
Precise positioning of the mitotic spindle is important for specifying the plane of cell division, which in turn determines how the cytoplasmic contents of the mother cell are partitioned into the daughter cells, and how the daughters are positioned within the tissue. During metaphase in the early Caenorhabditis elegans embryo, the spindle is aligned and centered on the anterior-posterior axis by a microtubule-dependent machinery that exerts restoring forces when the spindle is displaced from the center. To investigate the accuracy and stability of centering, we tracked the position and orientation of the mitotic spindle during the first cell division with high temporal and spatial resolution...
October 18, 2016: Biophysical Journal
https://www.readbyqxmd.com/read/27733624/stronger-net-posterior-cortical-forces-and-asymmetric-microtubule-arrays-produce-simultaneous-centration-and-rotation-of-the-pronuclear-complex-in-the-early-c-elegans-embryo
#9
Valerie C Coffman, Matthew B A McDermott, Blerta Shtylla, Adriana T Dawes
Positioning of microtubule organizing centers (MTOCs) incorporates biochemical and mechanical cues for proper alignment of the mitotic spindle and cell division site. Current experimental and theoretical studies in the early C. elegans embryo assume remarkable changes in the origin and polarity of forces acting on the MTOCs. These changes must occur over a few minutes, between initial centration and rotation of the pronuclear complex and entry into mitosis, and the models do not replicate in vivo timing of centration and rotation...
October 12, 2016: Molecular Biology of the Cell
https://www.readbyqxmd.com/read/27727303/cell-type-specific-responses-to-dna-replication-stress-in-early-c-elegans-embryos
#10
Holly Stevens, Ashley B Williams, W Matthew Michael
To better understand how the cellular response to DNA replication stress is regulated during embryonic development, we and others have established the early C. elegans embryo as a model system to study this important problem. As is the case in most eukaryotic cell types, the replication stress response is controlled by the ATR kinase in early worm embryos. In this report we use RNAi to systematically characterize ATR pathway components for roles in promoting cell cycle delay during a replication stress response, and we find that these genetic requirements vary, depending on the source of stress...
2016: PloS One
https://www.readbyqxmd.com/read/27711157/multisite-phosphorylation-of-numa-related-lin-5-controls-mitotic-spindle-positioning-in-c-elegans
#11
Vincent Portegijs, Lars-Eric Fielmich, Matilde Galli, Ruben Schmidt, Javier Muñoz, Tim van Mourik, Anna Akhmanova, Albert J R Heck, Mike Boxem, Sander van den Heuvel
During cell division, the mitotic spindle segregates replicated chromosomes to opposite poles of the cell, while the position of the spindle determines the plane of cleavage. Spindle positioning and chromosome segregation depend on pulling forces on microtubules extending from the centrosomes to the cell cortex. Critical in pulling force generation is the cortical anchoring of cytoplasmic dynein by a conserved ternary complex of Gα, GPR-1/2, and LIN-5 proteins in C. elegans (Gα-LGN-NuMA in mammals). Previously, we showed that the polarity kinase PKC-3 phosphorylates LIN-5 to control spindle positioning in early C...
October 2016: PLoS Genetics
https://www.readbyqxmd.com/read/27701073/a-continuum-of-mrnp-complexes-in-embryonic-microrna-mediated-silencing
#12
Edlyn Wu, Ajay A Vashisht, Clément Chapat, Mathieu N Flamand, Emiliano Cohen, Mihail Sarov, Yuval Tabach, Nahum Sonenberg, James Wohlschlegel, Thomas F Duchaine
MicroRNAs (miRNAs) impinge on the translation and stability of their target mRNAs, and play key roles in development, homeostasis and disease. The gene regulation mechanisms they instigate are largely mediated through the CCR4-NOT deadenylase complex, but the molecular events that occur on target mRNAs are poorly resolved. We observed a broad convergence of interactions of germ granule and P body mRNP components on AIN-1/GW182 and NTL-1/CNOT1 in Caenorhabditis elegans embryos. We show that the miRISC progressively matures on the target mRNA from a scanning form into an effector mRNP particle by sequentially recruiting the CCR4-NOT complex, decapping and decay, or germ granule proteins...
October 3, 2016: Nucleic Acids Research
https://www.readbyqxmd.com/read/27695653/mechanism-of-chromatin-segregation-to-the-nuclear-periphery-in-c-elegans-embryos
#13
COMMENT
Adriana Gonzalez-Sandoval, Susan M Gasser
In eukaryotic organisms, gene regulation occurs in the context of chromatin. In the interphase nucleus, euchromatin and heterochromatin occupy distinct space during cell differentiation, with heterochromatin becoming enriched at the nuclear and nucleolar peripheries. This organization is thought to fine-tune gene expression. To elucidate the mechanisms that govern this level of genome organization, screens were carried out in C. elegans which monitored the loss of heterochromatin sequestration at the nuclear periphery...
2016: Worm
https://www.readbyqxmd.com/read/27684088/ordering-single-cells-and-single-embryos-in-3d-confinement-a-new-device-for-high-content-screening
#14
Viktoria Wollrab, David Caballero, Raghavan Thiagarajan, Daniel Riveline
Biological cells are usually observed on flat (2D) surfaces. This condition is not physiological, and phenotypes and shapes are highly variable. Screening based on cells in such environments have therefore serious limitations: cell organelles show extreme phenotypes, cell morphologies and sizes are heterogeneous and/or specific cell organelles cannot be properly visualized. In addition, cells in vivo are located in a 3D environment; in this situation, cells show different phenotypes mainly because of their interaction with the surrounding extracellular matrix of the tissue...
2016: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/27672093/mitotic-spindle-positioning-in-the-ems-cell-of-caenorhabditis-elegans-requires-let-99-and-lin-5-numa
#15
Malgorzata J Liro, Lesilee S Rose
Asymmetric divisions produce daughter cells with different fates, and thus are critical for animal development. During asymmetric divisions, the mitotic spindle must be positioned on a polarized axis to ensure the differential segregation of cell fate determinants into the daughter cells. In many cell types a cortically localized complex consisting of Gα, GPR-1/2, and LIN-5 (Gαi/Pins/Mud, Gαi/LGN/NuMA) mediates the recruitment of dynactin/dynein, which exerts pulling forces on astral microtubules to physically position the spindle...
September 26, 2016: Genetics
https://www.readbyqxmd.com/read/27664515/zinc-availability-during-germline-development-impacts-embryo-viability-in-caenorhabditis-elegans
#16
Adelita D Mendoza, Teresa K Woodruff, Sarah M Wignall, Thomas V O'Halloran
Zinc is an essential metal that serves as a cofactor in a variety of cellular processes, including meiotic maturation. Cellular control of zinc uptake, availability and efflux is closely linked to meiotic progression in rodent and primate reproduction where large fluctuations in zinc levels are critical at several steps in the oocyte-to-embryo transition. Despite these well-documented roles of zinc fluxes during meiosis, only a few of the genes encoding key zinc receptors, membrane-spanning transporters, and downstream signaling pathway factors have been identified to date...
September 21, 2016: Comparative Biochemistry and Physiology. Toxicology & Pharmacology: CBP
https://www.readbyqxmd.com/read/27663471/zinc-deficiency-reduces-fertility-in-c-elegans-hermaphrodites-and-disrupts-oogenesis-and-meiotic-progression
#17
James Hester, Wendy Hanna-Rose, Francisco Diaz
Zinc is necessary for successful gametogenesis in mammals; however the role of zinc in the gonad function of non-mammalian species has not been investigated. The genetic tractability, short generation time, and hermaphroditic reproduction of the nematode C. elegans offer distinct advantages for the study of impaired gametogenesis as a result of zinc deficiency. However the phenotypic reproductive effects arising from zinc restriction have not been established in this model. We therefore examined the effect of zinc deficiency on C...
September 20, 2016: Comparative Biochemistry and Physiology. Toxicology & Pharmacology: CBP
https://www.readbyqxmd.com/read/27646689/uncoupling-protein-ucp-4-may-be-involved-in-neuronal-defects-during-aging-and-resistance-to-pathogens-in-caenorhabditis-elegans
#18
Injeong Cho, Gyu Jin Hwang, Jeong Hoon Cho
Uncoupling proteins (UCPs) are mitochondrial inner membrane proteins that function to dissipate proton motive force and mitochondrial membrane potential. One UCP has been identified in Caenorhabditis elegans (C. elegans), namely UCP-4. In this study, we examined its expression and localization using a GFP marker in C. elegans. ucp-4 was expressed throughout the body from early embryo to aged adult and UCP-4 was localized in the mitochondria. It is known that increased mitochondrial membrane protential leads to a reactive oxygen species (ROS) increase, which is associated with age-related diseases, including neurodegenerative diseases in humans...
September 2016: Molecules and Cells
https://www.readbyqxmd.com/read/27638466/c30f12-4-influences-oogenesis-fat-metabolism-and-lifespan-in-c-elegans
#19
Lu Wang, Fei Xu, Guishuan Wang, Xiaorong Wang, Ajuan Liang, Hefeng Huang, Fei Sun
Reproduction, fat metabolism, and longevity are intertwined regulatory axes; recent studies in C. elegans have provided evidence that these processes are directly coupled. However, the mechanisms by which they are coupled and the reproductive signals modulating fat metabolism and lifespan are poorly understood. Here, we find that an oogenesis-enriched gene, c30f12.4, is specifically expressed and located in germ cells and early embryos; when the gene is knocked out, oogenesis is disrupted and brood size is decreased...
October 2016: Protein & Cell
https://www.readbyqxmd.com/read/27635036/caenorhabditis-elegans-glutamylating-enzymes-function-redundantly-in-male-mating
#20
Daniel G Chawla, Ruchi V Shah, Zachary K Barth, Jessica D Lee, Katherine E Badecker, Anar Naik, Megan M Brewster, Timothy P Salmon, Nina Peel
Microtubule glutamylation is an important modulator of microtubule function and has been implicated in the regulation of centriole stability, neuronal outgrowth and cilia motility. Glutamylation of the microtubules is catalyzed by a family of tubulin tyrosine ligase-like (TTLL) enzymes. Analysis of individual TTLL enzymes has led to an understanding of their specific functions, but how activities of the TTLL enzymes are coordinated to spatially and temporally regulate glutamylation remains relatively unexplored...
September 15, 2016: Biology Open
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