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https://www.readbyqxmd.com/read/28549242/identification-of-related-substances-in-tofacitinib-citrate-by-lc-ms-techniques-for-synthetic-process-optimization
#1
Xiao Wu, Xuefang Zeng, Lei Wang, Taijun Hang, Min Song
A specific LC-MS method was developed for separation, identification and characterization of the process-related substances and degradation products in tofacitinib citrate. The separation was achieved on a LiChrospher C18 column (250mm×4.6mm, 5μm) by linear gradient elution of 0.1% ammonium acetate solution (pH adjusted to 4.0 by formic acid) and acetonitrile at a flow rate of 1.0mL/min. Forced degradation studies were conducted under hydrolytic (acidic, basic), oxidative, photolytic and thermal stress conditions as described in ICH...
May 13, 2017: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/28547918/exercise-with-weight-loss-improves-adipose-tissue-and-skeletal-muscle-markers-of-fatty-acid-metabolism-in-postmenopausal-women
#2
Heidi K Ortmeyer, Andrew P Goldberg, Alice S Ryan
OBJECTIVE: The effects of 6-month weight loss (WL) versus aerobic exercise training (AEX)+WL on fat and skeletal muscle markers of fatty acid metabolism were determined in normal (NGT) and impaired (IGT) glucose tolerant African-American and Caucasian postmenopausal women with overweight/obesity. METHODS: Fat (gluteal and abdominal) lipoprotein lipase (LPL), skeletal muscle LPL, acyl-CoA synthase (ACS), ß-hydroxacyl-CoA dehydrogenase, carnitine palmitoyltransferase (CPT-1), and citrate synthase (CS) activities were measured at baseline (n = 104) and before and after WL (n = 34) and AEX+WL (n = 37)...
May 26, 2017: Obesity
https://www.readbyqxmd.com/read/28547654/potential-teratogenic-effects-of-clomiphene-citrate
#3
REVIEW
Alessandra Scaparrotta, Francesco Chiarelli, Alberto Verrotti
Clomiphene citrate (CC) is the oldest drug used to regulate the process of ovulation. Considering the great use of CC over the last 40 years, it is important to understand the possible risks associated with its use. The aim of this review was to evaluate the possible teratogenic effects of CC, analyzing results obtained from animal and human studies. The pharmacokinetics of CC and possible mechanisms involved in teratogenesis are examined. Fetal exposure to CC is possible due to the long half-life of CC and its metabolites...
May 25, 2017: Drug Safety: An International Journal of Medical Toxicology and Drug Experience
https://www.readbyqxmd.com/read/28543387/the-hematocrit-affects-the-volume-of-plasma-treated-with-coupled-plasma-filtration-and-adsorption-with-predilution
#4
Stefano Finazzi, Elena Garbero, Giampietro Trussardi, Guido Bertolini
Coupled plasma filtration and adsorption (CPFA) is an extracorporeal blood purification technique proposed for the treatment of septic-shock. By removing pro- and anti-inflammatory mediators from plasma, CPFA is supposed to have a therapeutic effect on the abnormal inflammatory response seen in this condition. Recently, blood predilution with citrate solution has been adopted to prevent clotting in the CPFA circuit-one of the main problems of the technique. Taking into account the patient's hematocrit, we worked out a formula for the volume of plasma effectively treated by CPFA after predilution...
May 2017: Artificial Organs
https://www.readbyqxmd.com/read/28542804/assessment-of-an-engineered-endothelium-via-single-photon-emission-computed-tomography
#5
Bin Jiang, Yidi Wu, Chad R Haney, Chongwen Duan, Guillermo A Ameer
The clinical translation of cell-based therapeutics often requires highly sensitive, non-invasive imaging tools to assess cell function and distribution in vivo. The objective of this research was to determine whether human Sodium-Iodide Symporter (hNIS) ectopic expression in endothelial cells (ECs) in combination with single-photon emission computed tomography (SPECT) is a feasible approach to non-invasively monitor the presence and viability of an engineered endothelium on expanded polytetrafluoroethylene (ePTFE)...
May 22, 2017: Biotechnology and Bioengineering
https://www.readbyqxmd.com/read/28542252/metabolic-flux-analysis-of-heterotrophic-growth-in-chlamydomonas-reinhardtii
#6
Nanette R Boyle, Neelanjan Sengupta, John A Morgan
Despite the wealth of knowledge available for C. reinhardtii, the central metabolic fluxes of growth on acetate have not yet been determined. In this study, 13C-metabolic flux analysis (13C-MFA) was used to determine and quantify the metabolic pathways of primary metabolism in C. reinhardtii cells grown under heterotrophic conditions with acetate as the sole carbon source. Isotopic labeling patterns of compartment specific biomass derived metabolites were used to calculate the fluxes. It was found that acetate is ligated with coenzyme A in the three subcellular compartments (cytosol, mitochondria and plastid) included in the model...
2017: PloS One
https://www.readbyqxmd.com/read/28539608/development-and-validation-of-a-high-throughput-whole-blood-thrombolysis-plate-assay
#7
T Bonnard, L S Law, Z Tennant, C E Hagemeyer
The objective of this work was to develop a high throughput assay for testing in vitro the thrombolytic activity using citrated whole blood samples, and to overcome the limitations of currently available techniques. We successfully developed a method that involves forming halo shaped, tissue factor induced, whole blood clots in 96 well plates, and then precisely measuring the thrombolysis process with a spectrophotometer plate reader. We here describe the implementation of this novel method, which we refer to as halo assay, and its validation with plasmin, urokinase and tissue plasminogen activator at different doses...
May 24, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28538441/hyperlactatemia-lactate-kinetics-and-prediction-of-citrate-accumulation-in-critically-ill-patients-undergoing-continuous-renal-replacement-therapy-with-regional-citrate-anticoagulation
#8
Dmytro Khadzhynov, Annette Dahlinger, Christin Schelter, Harm Peters, Detlef Kindgen-Milles, Klemens Budde, Lukas Johannes Lehner, Fabian Halleck, Oliver Staeck, Torsten Slowinski
OBJECTIVES: Citrate accumulation is a major complication of regional citrate anticoagulation during continuous renal replacement therapy. We studied the prediction of citrate accumulation during continuous veno-venous hemodialysis with regional citrate anticoagulation by initial lactate concentrations and lactate kinetics. DESIGN: A retrospective follow-up analysis from a cohort of critically ill patients. SETTING: Mixed medical-surgical ICUs at a university hospital...
May 22, 2017: Critical Care Medicine
https://www.readbyqxmd.com/read/28537659/metformin-improves-the-glucose-and-lipid-metabolism-via-influencing-the-level-of-serum-total-bile-acids-in-rats-with-streptozotocin-induced-type-2-diabetes-mellitus
#9
X-M Meng, X-X Ma, Y-L Tian, Q Jiang, L-L Wang, R Shi, L Ding, S-G Pang
OBJECTIVE: To study the effects of metformin on streptozotocin-induced type 2 diabetes mellitus (T2DM) in rats. MATERIALS AND METHODS: Wistar male rats were divided into two groups: standard diet (SD, n = 20) group and high-fat diet (HFD, n = 80) group. Twenty rats in HFD group were randomly treated with metformin (EI group). After 6 weeks, among rats in HFD group, 20 rats were intraperitoneally injected with citrate buffered saline (IR group), 20 rats treated with metformin per day for 4 weeks (LI group), and 20 rats were given nothing (DM group)...
May 2017: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/28537499/vitamin-d-improves-the-content-of-tgf-%C3%AE-and-igf-1-in-intervertebral-disc-of-diabetic-rats
#10
Ji-Long An, Wei Zhang, Jian Zhang, Li-Chao Lian, Yong Shen, Wen-Yuan Ding
OBJECTIVE: The contents of transforming growth factor-β and insulin-like growth factor-1 in disc of diabetic rats were measured at three different periods after injected with 1,25-Dihydroxyvitamin D3, and compared with that in normal rats. The significance of content changes was also discussed. METHODS: Fourty-five Sprague-Dawley (SD) rats were divided into three groups, namely the experimental group (STZ+calcitriol), control group (STZ+citrate buffer), and normal group (citrate buffer)...
January 1, 2017: Experimental Biology and Medicine
https://www.readbyqxmd.com/read/28537440/metabolic-characteristics-and-risks-associated-with-stone-recurrence-in-korean-young-adult-stone-patients
#11
Ho Won Kang, Sung Pil Seo, Won Tae Kim, Yong-June Kim, Seok Joong Yun, Wun-Jae Kim, Sang-Cheol Lee
PURPOSE The aim of the present study was to assess the metabolic characteristics and risk of stone recurrence in young adult stone patients in Korea. PATIENTS AND METHODS The medical records of 1,532 patients presenting with renal or ureteric stones at our stone clinic between 1994 and 2015 were retrospectively reviewed. Patients were grouped according to age (young adult, 18-29yr; intermediate onset, 30-59yr; old age, ≥ 60 yr) at first presentation and measurements of clinicometabolic characteristics and risks of stone recurrence were compared...
May 24, 2017: Journal of Endourology
https://www.readbyqxmd.com/read/28536342/-a-case-of-late-recurrence-of-breast-cancer-causing-multiple-organ-metastases-27-years-postoperatively
#12
Kimiyasu Yoneyama, Hiroshi Nakamura, Naotaka Tobe
The patient was a 77-year-old female with a history of treatment for cancer of the right breast 27 years earlier, who presented with a chief complaint of a subcutaneous mass in the left lower quadrant of her abdomen. A thorough examination revealed high breast cancer-related tumor marker values, and based on a computed tomography(CT)examination, a tentative diagnosis of multiple lung, liver, retroperitoneal, and abdominal wall metastases was made. Although there were multiple organ metastases, in view of the long interval before the recurrence, absence of any symptoms, and the patient's age, we initiated treatment by weekly administration of an aromatase inhibitor and trastuzumab...
May 2017: Gan to Kagaku Ryoho. Cancer & Chemotherapy
https://www.readbyqxmd.com/read/28535757/intracellular-product-recycling-in-high-succinic-acid-producing-yeast-at-low-ph
#13
S Aljoscha Wahl, Cristina Bernal Martinez, Zheng Zhao, Walter M van Gulik, Mickel L A Jansen
BACKGROUND: The metabolic engineering of Saccharomyces cerevisiae for the production of succinic acid has progressed dramatically, and a series of high-producing hosts are available. At low cultivation pH and high titers, the product transport can become bidirectional, i.e. the acid is reentering the cell and is again exported or even catabolized. Here, a quantitative approach for the identification of product recycling fluxes is developed. RESULTS: The metabolic flux distributions at two time-points of the fermentation process were analyzed...
May 23, 2017: Microbial Cell Factories
https://www.readbyqxmd.com/read/28535516/citrate-anticoagulation-during-continuous-renal-replacement-therapy
#14
Davide Ricci, Laura Panicali, Maria Grazia Facchini, Elena Mancini
During extracorporeal dialysis, some anticoagulation strategy is necessary to prevent the coagulation of blood. Heparin has historically been used as an anticoagulant because of its efficacy combined with low cost. However, a variable incidence of hemorrhagic complications (5-30%) has been documented in patients undergoing continuous renal replacement therapy (CRRT) with heparin as an anticoagulant. Citrate has anticoagulation properties secondary to its ability to chelate calcium, which is necessary for the coagulation cascade...
2017: Contributions to Nephrology
https://www.readbyqxmd.com/read/28535438/determination-of-edoxaban-equivalent-concentrations-in-human-plasma-by-an-automated-anti-factor-xa-chromogenic-assay
#15
Ling He, Jarema Kochan, Min Lin, Alexander Vandell, Karen Brown, Francois Depasse
INTRODUCTION: This phase I, open-label, multiple-dose, two-treatment study assessed the relationship between edoxaban equivalent concentration derived from an anti-FXa assay with the summed concentration of edoxaban and its active metabolite, M-4, as assessed by liquid chromatography coupled with tandem mass spectrometry (LC/MS/MS). This study also assessed the relationship between edoxaban plasma concentrations assessed by LC/MS/MS in sodium citrate and lithium heparin tubes. MATERIALS AND METHODS: Healthy volunteers were randomized to receive once-daily edoxaban 60mg or 90mg for 5days (15 participants per treatment group)...
May 7, 2017: Thrombosis Research
https://www.readbyqxmd.com/read/28535303/a-novel-goldnano-carb-test-for-rapid-phenotypic-detection-of-carbapenemases-particularly-oxa-type-in-enterobacteriaceae-pseudomonas-aeruginosa-and-acinetobacter-spp
#16
Arpasiri Srisrattakarn, Aroonlug Lulitanond, Chotechana Wilailuckana, Nicha Charoensri, Jureerut Daduang, Aroonwadee Chanawong
Objectives: To develop a simple gold nanoparticle (AuNP)-based colorimetric test, GoldNano Carb (GoldC), for detecting carbapenemase production in Gram-negative bacteria, compared with updated Carba NP (CNP) and CarbAcineto NP (CAcNP) tests by using PCR methods as gold standard. Methods: Ninety-nine carbapenemase-producing Enterobacteriaceae (CPE), Pseudomonas spp. and Acinetobacter spp. isolates and 89 non-CPE isolates were tested by the GoldC and CNP. Additionally, the CAcNP was performed in the Acinetobacter spp...
May 23, 2017: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/28533422/paternal-hyperglycemia-in-rats-exacerbates-the-development-of-obesity-in-offspring
#17
Xiaoqin Shi, Xinyu Li, Yi Hou, Xuemei Cao, Yuyao Zhang, Heng Wang, Hongying Wang, Chuan Peng, Jibin Li, Qifu Li, Chaodong Wu, Xiaoqiu Xiao
Parental history with obesity or diabetes will increase the risk for developing metabolic diseases in offspring. However, literatures as to transgenerational inheritance of metabolic dysfunctions through male lineage are relatively scarce. In current study, we aimed to evaluate influences of paternal hyperglycemia on metabolic phenotypes in offspring. Male SD rats were ip injected with streptozotocin (STZ) or citrate buffer (CB, as control). STZ-injected rats with glucose levels higher than 16 mM were selected to breed with normal female rats...
May 22, 2017: Journal of Endocrinology
https://www.readbyqxmd.com/read/28533270/classical-activation-of-macrophages-and-vardenafil
#18
Richmond Muimo
Inhibitors of phosphodiesterase 5 (PDE5) - sildenafil citrate (Viagra; Pfizer) and vardenafil hydrochloride (Levitra; Bayer/GlaxoSmithKline) - approved for the treatment of erectile dysfunction and pulmonary arterial hypertension also rescue the loss of cystic fibrosis (CF) chloride channel function and the mislocalization of F508del-CFTR in affected tissues in CF. Can PDE5 inhibitors provide a therapeutic strategy which combines ability to correct the basic ion transport defect and to control de-regulated lung inflammation in CF?...
June 1, 2017: Clinical Science (1979-)
https://www.readbyqxmd.com/read/28532835/effect-of-cholesterol-loaded-cyclodextrin-supplementation-on-tyrosine-phosphorylation-and-apoptosis-like-changes-in-frozen-thawed-hariana-bull-spermatozoa
#19
Hanuman P Yadav, Anuj Kumar, Nadeem Shah, Dharmendra S Chauhan, Atul Saxena, Sarvajeet Yadav, Dilip Kumar Swain
The beneficial effects of cholesterol loaded cyclodextrin (CLC) addition were evaluated in cryopreserved bull semen. Forty ejaculates were collected from Hariana bulls (n = 4), pooled and divided into 4 aliquots. All the aliquots were initially diluted in to egg yolk tris citrate and supplemented with CLC @ 0.5 mg (Group-II), 1.0 mg (G-III) and 2.0 mg (G-IV) CLC/120 × 10(6) spermatozoa or without CLC (G-I) that served as control. Extended semen was cryopreserved at -196 °C for 24 h. Seminal attributes like motility, viability, cryocapacitation like changes, tyrosine phosphorylation, apoptosis like changes in terms of mitochondrial transmembrane potential and DNA integrity were evaluated after equilibration and thawing...
July 1, 2017: Theriogenology
https://www.readbyqxmd.com/read/28532507/isoprenoid-biosynthesis-in-dandelion-latex-is-enhanced-by-the-overexpression-of-three-key-enzymes-involved-in-the-mevalonate-pathway
#20
Katharina M Pütter, Nicole van Deenen, Kristina Unland, Dirk Prüfer, Christian Schulze Gronover
BACKGROUND: Latex from the dandelion species Taraxacum brevicorniculatum contains many high-value isoprenoid end products, e.g. triterpenes and polyisoprenes such as natural rubber. The isopentenyl pyrophosphate units required as precursors for these isoprenoids are provided by the mevalonate (MVA) pathway. The key enzyme in this pathway is 3-hydroxy-methyl-glutaryl-CoA reductase (HMGR) and its activity has been thoroughly characterized in many plant species including dandelion. However, two enzymes acting upstream of HMGR have not been characterized in dandelion latex: ATP citrate lyase (ACL), which provides the acetyl-CoA utilized in the MVA pathway, and acetoacetyl-CoA thiolase (AACT), which catalyzes the first step in the pathway to produce acetoacetyl-CoA...
May 22, 2017: BMC Plant Biology
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