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https://www.readbyqxmd.com/read/28108664/an-investigation-of-the-genotoxicity-and-interference-of-gold-nanoparticles-in-commonly-used-in-vitro-mutagenicity-and-genotoxicity-assays
#1
Jiya M George, Millicent Magogotya, Melissa A Vetten, Antoinette V Buys, Mary Gulumian
The suitability of 4 in vitro assays, commonly used for mutagenicity and genotoxicity assessment, was investigated in relation to treatment with 14 nm citrate-stabilized gold nanoparticles (AuNPs). Specifically, the Ames test was conducted without metabolic activation, where no mutagenic effects were observed. High resolution transmission electron microscopy and Cytoviva dark-field image analysis showed that AuNPs did not enter the bacterial cells, thus confirming the unreliability of the Ames test for nanoparticle mutagenicity studies...
January 20, 2017: Toxicological Sciences: An Official Journal of the Society of Toxicology
https://www.readbyqxmd.com/read/28108444/cancer-risk-in-women-treated-with-fertility-drugs-according-to-parity-status-a-registry-based-cohort-study
#2
Marte M Reigstad, Ritsa Storeng, Tor A Myklebust, Nan B Oldereid, Anne K Omland, Trude E Robsahm, Louise A Brinton, Siri Vangen, Kari Furu, Inger K Larsen
Background Long-term safety of assisted reproductive techniques (ART) is of interest as use is increasing. Cancer risk is known to be affected by parity. This study examined risk of cancer after fertility treatment, stratified by women's parity. Methods Data was obtained on all women (n=1 353 724) born in Norway between 1960-1996. Drug exposure data (2004-2014) was obtained from the Norwegian Prescription Database [drugs used in ART and clomiphene citrate (CC)]. The Medical Birth Registry of Norway provided parity status...
January 20, 2017: Cancer Epidemiology, Biomarkers & Prevention
https://www.readbyqxmd.com/read/28107958/one-step-synthesis-of-graphitic-carbon-nitride-nanosheets-with-the-help-of-melamine-and-its-application-for-fluorescence-detection-of-mercuric-ions
#3
Qianfen Zhuang, Liming Sun, Yongnian Ni
A facile, simple, and relatively environment-friendly hydrothermal approach was developed for one-step synthesis of graphitic carbon nitride nanosheets (GCNNs) using melamine and sodium citrate as the precursors. The prepared GCNNs emit strong fluorescence with a high quantum yield of 48.3%. The GCNNs were then characterized by various techniques including transmission electron microscopy, atomic force microscopy, X-ray diffraction, Fourier transform infrared spectroscopy, and UV-Vis absorption spectroscopy...
March 1, 2017: Talanta
https://www.readbyqxmd.com/read/28107516/liver-amp-activated-protein-kinase-is-unnecessary-for-gluconeogenesis-but-protects-energy-state-during-nutrient-deprivation
#4
Clinton M Hasenour, D Emerson Ridley, Freyja D James, Curtis C Hughey, E Patrick Donahue, Benoit Viollet, Marc Foretz, Jamey D Young, David H Wasserman
AMPK is an energy sensor that protects cellular energy state by attenuating anabolic and promoting catabolic processes. AMPK signaling is purported to regulate hepatic gluconeogenesis and substrate oxidation; coordination of these processes is vital during nutrient deprivation or pathogenic during overnutrition. Here we directly test hepatic AMPK function in regulating metabolic fluxes that converge to produce glucose and energy in vivo. Flux analysis was applied in mice with a liver-specific deletion of AMPK (L-KO) or floxed control littermates to assess rates of hepatic glucose producing and citric acid cycle (CAC) fluxes...
2017: PloS One
https://www.readbyqxmd.com/read/28107068/successful-treatment-of-suspected-pulmonary-arterial-hypertension-in-a-mealy-amazon-parrot-amazona-farinose
#5
Sean M Brady, Anne Burgdorf-Moisuk, Sarah Silverman, Raymund F Wack
A 25-year-old, male mealy Amazon parrot (Amazona farinose) with a history of polycythemia, hepatomegaly, and epistaxis was evaluated for progressive lethargy and anorexia. Clinical laboratory testing revealed severe polycythemia (71%), hypophosphatemia (1.6 mg/dL), and mild hypokalemia (2.8 mEq/L). Radiographs showed marked hepatomegaly and loss of air sac space. Despite supportive treatments, the bird's condition deteriorated, and it developed ataxia, was unable to fly, and became oxygen dependent. An echocardiogram, including an air bubble study, revealed a right-to-left atrial shunt and presumed pulmonary arterial hypertension...
December 2016: Journal of Avian Medicine and Surgery
https://www.readbyqxmd.com/read/28106122/comparative-genomics-and-transcriptome-analysis-of-aspergillus-niger-and-metabolic-engineering-for-citrate-production
#6
Xian Yin, Hyun-Dong Shin, Jianghua Li, Guocheng Du, Long Liu, Jian Chen
Despite a long and successful history of citrate production in Aspergillus niger, the molecular mechanism of citrate accumulation is only partially understood. In this study, we used comparative genomics and transcriptome analysis of citrate-producing strains-namely, A. niger H915-1 (citrate titer: 157 g L(-1)), A1 (117 g L(-1)), and L2 (76 g L(-1))-to gain a genome-wide view of the mechanism of citrate accumulation. Compared with A. niger A1 and L2, A. niger H915-1 contained 92 mutated genes, including a succinate-semialdehyde dehydrogenase in the γ-aminobutyric acid shunt pathway and an aconitase family protein involved in citrate synthesis...
January 20, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28104921/effects-of-gold-nanoparticles-on-lipid-packing-and-membrane-pore-formation
#7
Anupama Bhat, Lance W Edwards, Xiao Fu, Dillon L Badman, Samuel Huo, Albert J Jin, Qi Lu
Gold nanoparticles (AuNPs) have been increasingly integrated in biological systems, making it imperative to understand their interactions with cell membranes, the first barriers to be crossed to enter cells. Herein, liposomes composed of 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) as a model membrane system were treated with citrate stabilized AuNPs from 5 to 30 nm at various concentrations. The fluorescence shifts of Laurdan probes reveal that AuNPs in general made liposomes more fluidic. The increased fluidity is expected to result in an increased surface area, and thus liposome shape changes from circular to less circular, which was further confirmed with fluorescence microscopy...
December 26, 2016: Applied Physics Letters
https://www.readbyqxmd.com/read/28104667/carbon-catabolite-repression-and-cell-dispersal-affects-degradation-of-the-xenobiotic-compound-3-4-dichloroaniline-in-comamonas-testosteroni-wdl7-biofilms
#8
Benjamin Horemans, Philip Breugelmans, Johan Hofkens, Dirk Springael
Organic pollutant degrading biofilms in natural ecosystems and water treatment systems are often exposed to other carbon sources in addition to the pollutant. The availability of auxilliary carbon sources can lead to surplus biomass growth, changes in biofilm structure and carbon catabolite repression (CCR) which together will affect pollutant degradation rate and efficiency of the system. To understand the interplay between these processes, continuous biofilms of the 3,4-dichloroaniline (3,4-DCA) degrading Comamonas testosteroni WDL7-RFP were grown in single- and dual-substrate conditions with 3,4-DCA and/or citrate and reciprocal effects on 3,4-DCA/citrate degradation, biofilm biomass and biofilm structure were examined...
January 18, 2017: FEMS Microbiology Ecology
https://www.readbyqxmd.com/read/28104108/persisting-hypocalcemia-after-surgical-parathyroidectomy-the-differential-effectiveness-of-calcium-citrate-versus-calcium-carbonate-with-acid-suppression
#9
Sabahat Afshan, Abdeen R Farah Musa, Vonda Echols, Anna A Lerant, Tibor Fülöp
The effectiveness of oral calcium (Ca) may be contingent on a patient׳s factors beyond compliance, such as proton-pump inhibitor use and the choice of calcium supplements. A 32-year-old Hispanic male with end-stage renal disease on peritoneal dialysis underwent successful surgical parathyroidectomy (intact parathyroid hormone level: 2,328pg/mL; postsurgical: 287-69pg/mL [normal: 8.5-72.5]). His postoperative course was complicated by severe and recurrent hypocalcemia as outpatient and he needed repeated admissions for intravenous Ca gluconate...
January 2017: American Journal of the Medical Sciences
https://www.readbyqxmd.com/read/28103994/-analysis-of-microrna-expression-profile-in-serum-of-patients-with-electrical-burn-or-thermal-burn
#10
Q F Ruan, M J Jiang, Z Q Ye, C L Zhao, W G Xie
Objective: To explore the differential expression of microRNAs in the serum among patients with electrical burn or thermal burn and healthy persons and to explore the significance. Methods: In this study we included three patients with electrical burn and three patients with thermal burn, conforming to the inclusion criteria and hospitalized in our burn ward from June to August 2015, and three healthy adult volunteers. Their serum samples were separated from whole blood and divided into electrical burn group, thermal burn group, and normal control group...
January 20, 2017: Zhonghua Shao Shang za Zhi, Zhonghua Shaoshang Zazhi, Chinese Journal of Burns
https://www.readbyqxmd.com/read/28103427/repeated-maximal-intensity-hypoxic-exercise-superimposed-to-hypoxic-residence-boosts-skeletal-muscle-transcriptional-responses-in-elite-team-sport-athletes
#11
Franck Brocherie, Grégoire P Millet, Gommaar D'Hulst, Ruud Van Thienen, Louise Deldicque, Olivier Girard
AIM: To determine whether repeated maximal-intensity hypoxic exercise induces larger beneficial adaptations on the hypoxia inducible factor-1α pathway and its target genes than similar normoxic exercise, when combined with chronic hypoxic exposure. METHODS: Lowland elite male team-sport athletes underwent 14 days of passive normobaric hypoxic exposure (≥14 h.day(-1) at Fi O2 14.5-14.2%) with the addition of six maximal-intensity exercise sessions either in normobaric hypoxia (Fi O2 ~14...
January 19, 2017: Acta Physiologica
https://www.readbyqxmd.com/read/28101935/effect-of-cholesterol-loaded-cyclodextrins-on-cryosurvival-of-dog-spermatozoa
#12
J Khan, M Z Tahir, A Khalid, A Sattar, N Ahmad
Cryopreservation affects integrity of cholesterol and phospholipids in the plasma membrane of sperm leading to decreased fertility of frozen-thawed semen. Cholesterol-loaded cyclodextrins (CLC) have been shown to improve post-thaw semen quality in various species. The aim of this study was to investigate the optimal concentration of CLC for better post-thaw semen quality in dogs. Semen collection, through digital manipulation, was conducted once a week in four adult German shepherd dogs (n = 20 ejaculates; five ejaculates/dog)...
January 18, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/28101806/purification-and-characterization-of-glutamate-decarboxylase-from-enterococcus-raffinosus-tccc11660
#13
Chuanyou Chang, Jun Zhang, Shenxi Ma, Lin Wang, Depei Wang, Jian Zhang, Qiang Gao
Glutamate decarboxylase (GAD) is the sole enzyme that synthesizes γ-aminobutyric acid through the irreversible decarboxylation of L-glutamate. In this study, the purification and characterization of an unreported GAD from a novel strain of Enterococcus raffinosus TCCC11660 were investigated. The native GAD from E. raffinosus TCCC11660 was purified 32.3-fold with a recovery rate of 8.3%, using ultrafiltration and ammonium sulfate precipitation, followed by ion-exchange and size-exclusion chromatography. The apparent molecular weight of purified GAD, as determined by SDS-PAGE and size-exclusion chromatography was 55 and 110 kDa, respectively, suggesting that GAD exists as a dimer of identical subunits in solution...
January 18, 2017: Journal of Industrial Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28098465/rejection-of-commonly-used-electrolyte-in-asymmetric-flow-field-flow-fractionation-effects-of-membrane-molecular-weight-cut-off-size-fluid-dynamics-and-valence-of-electrolytes
#14
Thilak K Mudalige, Haiou Qu, Sean W Linder
Asymmetric flow field flow fractionation (AF4) is an efficient size based separation technique for the characterization of submicron size particulates. In AF4, membranes having various molecular weight cut-off sizes are used as a barrier to retain particles while allowing carrier fluid containing electrolytes to permeate. Here, we hypothesized that electrolyte rejection by the barrier membrane lead to the accumulation of the electrolytes in the channel during operation. Electrolyte accumulation can cause various adverse effects which can lead to membrane fouling...
January 18, 2017: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/28098230/molecular-mechanism-of-the-allosteric-regulation-of-the-%C3%AE-%C3%AE-heterodimer-of-human-nad-dependent-isocitrate-dehydrogenase
#15
Tengfei Ma, Yingjie Peng, Wei Huang, Jianping Ding
Human NAD-dependent isocitrate dehydrogenase catalyzes the decarboxylation of isocitrate (ICT) into α-ketoglutarate in the Krebs cycle. It exists as the α2βγ heterotetramer composed of the αβ and αγ heterodimers. Previously, we have demonstrated biochemically that the α2βγ heterotetramer and αγ heterodimer can be allosterically activated by citrate (CIT) and ADP. In this work, we report the crystal structures of the αγ heterodimer with the γ subunit bound without or with different activators...
January 18, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28097715/c-di-amp-modulates-listeria-monocytogenes-central-metabolism-to-regulate-growth-antibiotic-resistance-and-osmoregulation
#16
Aaron T Whiteley, Nicholas E Garelis, Bret N Peterson, Philip H Choi, Liang Tong, Joshua J Woodward, Daniel A Portnoy
Cyclic di-adenosine monophosphate (c-di-AMP) is a conserved nucleotide second messenger critical for bacterial growth and resistance to cell wall-active antibiotics. In Listeria monocytogenes, the sole diadenylate cyclase, DacA, is essential in rich, but not synthetic media and ΔdacA mutants are highly sensitive to the β-lactam antibiotic cefuroxime. In this study, loss of function mutations in the oligopeptide importer (oppABCDF) and glycine betaine importer (gbuABC) allowed ΔdacA mutants to grow in rich medium...
January 18, 2017: Molecular Microbiology
https://www.readbyqxmd.com/read/28096432/effects-of-activity-genetic-selection-and-their-interaction-on-muscle-metabolic-capacities-and-organ-masses-in-mice
#17
Scott A Kelly, Fernando R Gomes, Erik M Kolb, Jessica L Malisch, Theodore Garland
Chronic voluntary exercise elevates total daily energy expenditure (DEE) and food consumption, potentially resulting in organ compensation supporting nutrient extraction/utilization. Additionally, species with naturally higher DEE often have larger processing organs, which may represent genetic differences and/or phenotypic plasticity. We tested for possible adaptive changes in organ masses of 4 replicate lines of house mice selected (37 generations) for high running (HR lines) compared with 4 non-selected control (C) lines...
January 17, 2017: Journal of Experimental Biology
https://www.readbyqxmd.com/read/28095484/experimental-diabetes-alters-the-morphology-and-nano-structure-of-the-achilles-tendon
#18
Rodrigo Ribeiro de Oliveira, Rômulo Medina de Mattos, Luciana Magalhães Rebelo, Fernanda Guimarães Meireles Ferreira, Fernanda Tovar-Moll, Luiz Eurico Nasciutti, Gerly Anne de Castro Brito
Although of several studies that associate chronic hyperglycemia with tendinopathy, the connection between morphometric changes as witnessed by magnetic resonance (MR) images, nanostructural changes, and inflammatory markers have not yet been fully established. Therefore, the present study has as a hypothesis that the Achilles tendons of rats with diabetes mellitus (DM) exhibit structural changes. The animals were randomly divided into two experimental groups: Control Group (n = 06) injected with a vehicle (sodium citrate buffer solution) and Diabetic Group (n = 06) consisting of rats submitted to intraperitoneal administration of streptozotocin...
2017: PloS One
https://www.readbyqxmd.com/read/28093934/efficacy-and-safety-of-oral-sildenafil-in-treatment-of-pediatric-head-and-neck-lymphatic-malformations
#19
Shengcai Wang, Jie Zhang, Wentong Ge, Yuanhu Liu, Yongli Guo, Yue Liu, Ning Wang, Yuzhu He, Jun Tai, Xin Ni
CONCLUSIONS: Low dose oral sildenafil citrate tablets are safe and effective to decrease the lesions volume in some LMs children, especially with macrocystic lesion or mixed type. OBJECTIVE: To evaluate the efficacy and safety of oral sildenafil in treatment of pediatric head and neck lymphatic malformations (LMs). SUBJECTS AND METHODS: In this open-label study, 21 children diagnosed with LMs were given oral sildenafil and stopped at week 24...
January 17, 2017: Acta Oto-laryngologica
https://www.readbyqxmd.com/read/28091955/genome-wide-exploration-of-silicon-si-transporter-genes-lsi1-and-lsi2-in-plants-insights-into-si-accumulation-status-capacity-of-plants
#20
Recep Vatansever, Ibrahim Ilker Ozyigit, Ertugrul Filiz, Nermin Gozukara
Silicon (Si) is a nonessential, beneficial micronutrient for plants. It increases the plant stress tolerance in relation to its accumulation capacity. In this work, root Si transporter genes were characterized in 17 different plants and inferred for their Si-accumulation status. A total of 62 Si transporter genes (31 Lsi1 and 31 Lsi2) were identified in studied plants. Lsi1s were 261-324 residues protein with a MIP family domain whereas Lsi2s were 472-547 residues with a citrate transporter family domain. Lsi1s possessed characteristic sequence features that can be employed as benchmark in prediction of Si-accumulation status/capacity of the plants...
January 13, 2017: Biometals: An International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine
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