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Adaptive RT

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https://www.readbyqxmd.com/read/28228493/hepatitis-e-virus-detection-and-quantification-by-an-rt-qpcr-assay-calibrated-to-the-world-health-organization-standard-for-hev-rna
#1
Jeffrey J Germer, Irina Ankoudinova, Yevgeniy S Belousov, Walt Mahoney, Chen Dong, Jihong Meng, Jayawant N Mandrekar, Joseph D Yao
Hepatitis E virus (HEV) has emerged as a cause of chronic hepatitis among immunocompromised patients. Molecular assays have become important tools for the diagnosis and management of these chronically-infected patients. A reverse transcription, real-time PCR (RT-qPCR) assay utilizing Pleiades probe chemistry and an RNA internal control for simultaneous detection and quantification of HEV RNA in human serum was developed based on adaptation of a previously described and broadly reactive primer set targeting the overlapping open reading frame (ORF) 2/3 nucleotide sequence of HEV...
February 22, 2017: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/28228417/oxygen-sensitive-regulation-and-neuroprotective-effects-of-growth-hormone-dependent-growth-factors-during-early-postnatal-development
#2
Susan Jung, Gudrun Boie, Helmuth-Guenther Doerr, Regina Trollmann
Perinatal hypoxia severely disrupts metabolic and somatotrophic development, as well as cerebral maturational programs. Hypoxia-inducible transcription factors (HIF) represent the most important endogenous adaptive mechanisms to hypoxia, activating a broad spectrum of growth factors that contribute to cell survival and energy homeostasis. To analyse effects of systemic hypoxia and growth hormone (GH) therapy (rhGH) on HIF-dependent growth factors during early postnatal development, we compared protein (ELISA) and mRNA levels (quantitative RT PCR) of growth factors in plasma and brain between normoxic and hypoxic mice (8% O2, 6 h; postnatal day 7, P7) at P14...
February 22, 2017: American Journal of Physiology. Regulatory, Integrative and Comparative Physiology
https://www.readbyqxmd.com/read/28224676/transcriptional-regulation-of-hb-%C3%AE-and-hb-%C3%AE-through-nuclear-factor-e2-related-factor-2-nrf2-activation-in-human-vaginal-cells-a-novel-mechanism-of-cellular-adaptability-to-oxidative-stress
#3
Debarchana Saha, Swanand Koli, Kudumula Venkata Rami Reddy
PROBLEM: Hemoglobin (Hb), a major protein involved in transport of oxygen (O2 ), is expressed by erythroid lineages. Until recently, it was not known whether non-erythroid cells express Hb. The objective was to evaluate the expression and functional significance of Hb-α and Hb-β in human primary vaginal epithelial cells (hPVECs) and decipher downstream signaling. METHODS OF STUDY: RT-PCR, qRT-PCR, flow cytometry, Western blot, immunofluorescence were used to evaluate the expression of Hb-α, Hb-β, and nuclear factor E2-related factor-2(Nrf2) after hydrogen peroxide (H2 O2 ) induction...
February 21, 2017: American Journal of Reproductive Immunology: AJRI
https://www.readbyqxmd.com/read/28211218/the-integration-of-mri-in-radiation-therapy-collaboration-of-radiographers-and-radiation-therapists
#4
Robba Rai, Shivani Kumar, Vikneswary Batumalai, Doaa Elwadia, Lucy Ohanessian, Ewa Juresic, Lynette Cassapi, Shalini K Vinod, Lois Holloway, Paul J Keall, Gary P Liney
The increased utilisation of magnetic resonance imaging (MRI) in radiation therapy (RT) has led to the implementation of MRI simulators for RT treatment planning and influenced the development of MRI-guided treatment systems. There is extensive literature on the advantages of MRI for tumour volume and organ-at-risk delineation compared to computed tomography. MRI provides both anatomical and functional information for RT treatment planning (RTP) as well as quantitative information to assess tumour response for adaptive treatment...
February 16, 2017: Journal of Medical Radiation Sciences
https://www.readbyqxmd.com/read/28195977/fat-grip-resistance-training-improves-driving-performance-in-division-i-male-golfers
#5
Patrick M Cummings, Hunter S Waldman, Ben M Krings, JohnEric W Smith, Matthew J McAllister
Fat Grip (FG) training is implemented into strength and conditioning programs with the overall goal of increasing grip strength. Previous research assessing the effect of training with increased grip diameters compared to standard Olympic bar diameters has mainly been in acute settings. Therefore, the purpose of this study was to examine to effects FG training compared to normal diameter grip (CON) training during an eight week periodized resistance training (RT) program in Division I male golfers. Subjects (n = 10) were randomly assigned into two groups: the FG group (n = 5, scoring average: 75...
February 8, 2017: Journal of Strength and Conditioning Research
https://www.readbyqxmd.com/read/28177289/hepatitis-e-virus-in-bottlenose-dolphins-tursiops-truncatus
#6
María Caridad Montalvo Villalba, Danilo Cruz Martínez, Imran Ahmad, Licel A Rodriguez Lay, Marite Bello Corredor, Celia Guevara March, Liena Sánchez Martínez, Laima Sánchez Martínez-Campo, Shahid Jameel
Hepatitis E virus (HEV) infects several animal species that act as zoonotic reservoirs for viral transmission. Solid and liquid residues from infected animals could lead to HEV contamination of food and surface waters. Evidence of human HEV infection through ingestion of seafood (shellfish, mussels) has been reported. Dolphins generally feed on fish and squid but are able to adapt to an environment and consume whatever prey is available. Clinical signs of infected dolphins include lethargy, inappetence, behavioral aberrations and increased serum alanine aminotransferase (ALT)...
February 8, 2017: Diseases of Aquatic Organisms
https://www.readbyqxmd.com/read/28176850/application-of-machine-learning-models-to-predict-tacrolimus-stable-dose-in-renal-transplant-recipients
#7
Jie Tang, Rong Liu, Yue-Li Zhang, Mou-Ze Liu, Yong-Fang Hu, Ming-Jie Shao, Li-Jun Zhu, Hua-Wen Xin, Gui-Wen Feng, Wen-Jun Shang, Xiang-Guang Meng, Li-Rong Zhang, Ying-Zi Ming, Wei Zhang
Tacrolimus has a narrow therapeutic window and considerable variability in clinical use. Our goal was to compare the performance of multiple linear regression (MLR) and eight machine learning techniques in pharmacogenetic algorithm-based prediction of tacrolimus stable dose (TSD) in a large Chinese cohort. A total of 1,045 renal transplant patients were recruited, 80% of which were randomly selected as the "derivation cohort" to develop dose-prediction algorithm, while the remaining 20% constituted the "validation cohort" to test the final selected algorithm...
February 8, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28169290/knockdown-of-a-cellulose-synthase-gene-boicesa-affects-the-leaf-anatomy-cellulose-content-and-salt-tolerance-in-broccoli
#8
Shuangtao Li, Lei Zhang, Ying Wang, Fengfeng Xu, Mengyun Liu, Peng Lin, Shuxin Ren, Rui Ma, Yang-Dong Guo
Cellulose is the major component of cell wall materials. A 300 bp specific fragment from the cDNA fragment was chosen to insert into vector pFGC1008 at forward and reverse orientations to construct the recombinant RNAi vector. Knockdown of BoiCesA caused "dwarf" phenotype with smaller leaves and a loss of the content of cellulose. Moreover, RT-PCR analysis confirmed that the expression of the RNAi apparatus could repress expression of the CesA gene. Meanwhile, examination of the leaves from the T3 of RNAi transformants indicated reduction of cell expansion in vascular bundles, particularly on their abaxial surface...
February 7, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28169043/the-value-of-adaptive-preoperative-radiotherapy-in-management-of-soft-tissue-sarcoma
#9
Colleen Dickie, Amy Parent, Anthony M Griffin, Jay Wunder, Peter Ferguson, Peter W Chung, Tim Craig, Michael Sharpe, Nathan Becker, David Shultz, Charles N Catton, Brian O'Sullivan
PURPOSE: To determine the value of preoperative adaptive radiotherapy (ART) for soft tissue sarcoma patients (STS) by modeling the dosimetric consequences of tumour volume changes (TVC) using different external beam radiotherapy techniques. METHODS AND MATERIALS: A subset of 22 STS patients from a recent trial (NCT00188175) underwent a repeat CT scan (CT2) prompted by TVC>1cm during IMRT; 14 tumours grew, 8 shrank. Conformal and conventional plans were modelled in addition to IMRT replicating original criteria from the initial planning dataset (CT1):95% PTV encompassed by 97% prescribed dose...
February 3, 2017: Radiotherapy and Oncology: Journal of the European Society for Therapeutic Radiology and Oncology
https://www.readbyqxmd.com/read/28167252/evolution-of-lymphocytes-immunoglobulin-t-of-the-teleost-sea-bass-dicentrarchus-labrax-quantitation-of-gene-expressing-and-immunoreactive-cells
#10
S Picchietti, N Nuñez-Ortiz, V Stocchi, E Randelli, F Buonocore, L Guerra, G Scapigliati
Immunoglobulin T (IgT) is one of the key effector molecules of jawed vertebrate's adaptive immune system, and in this work we describe the quantitative distribution of IgT-expressing and IgT-producing cells in tissues of the European seabass Dicentrarchus labrax by using mRNA riboprobes and a specific anti-IgT antibody. A polyclonal antiserum (pAb) was prepared by immunizing rabbits with three synthetic peptides deduced from the full length IgT cDNA sequence and located in a surface-exposed CH3 domain of IgT constant region...
February 3, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28158279/analysis-of-trpv-channel-activation-by-stimulation-of-fc%C3%AE%C2%B5ri-and-mrgpr-receptors-in-mouse-peritoneal-mast-cells
#11
A Solís-López, U Kriebs, A Marx, S Mannebach, W B Liedtke, M J Caterina, M Freichel, V V Tsvilovskyy
The activation of mast cells (MC) is part of the innate and adaptive immune responses and depends on Ca2+ entry across the plasma membrane, leading to the release of preformed inflammatory mediators by degranulation or by de novo synthesis. The calcium conducting channels of the TRPV family, known by their thermo and osmotic sensitivity, have been proposed to be involved in the MC activation in murine, rat, and human mast cell models. So far, immortalized mast cell lines and nonspecific TRPV blockers have been employed to characterize the role of TRPV channels in MC...
2017: PloS One
https://www.readbyqxmd.com/read/28152934/an-approach-to-implementing-meaningful-change-in-a-provincial-health-care-system
#12
Elizabeth Lockhart, Michael Donald Brundage, Margaret Hart, Mark Hartman, Sophie Foxcroft, Lindsay Elizabeth Reddeman, Carina Simniceanu, Marissa Mendelsohn, Lisa Favell, Jonathan Wang, Elaine Meertens, Eric Gutierrez, Padraig Richard Warde
: 120 Background: Radiation treatment (RT) is essential to cancer management, contributing to cure and symptom control. With increasing cancer incidence and treatment complexity, health systems must adapt to ensure patients (pts) receive the highest quality of care. METHODS: With the objective of ensuring equitable access to high-quality, safe care, Cancer Care Ontario (CCO), a provincial government agency, identified provincial variability in RT activities. As a result, CCO prioritized 3 quality initiatives over the past 7 years: 1) Access to Intensity Modulated RT (IMRT) (2008-2013); 2) Peer Review of RT plans due to increasing RT planning complexity and the existence of high-profile RT errors (2012-present); and 3) Ensuring equitable access to RT (RT Utilization) (2014-present)...
March 2016: Journal of Clinical Oncology: Official Journal of the American Society of Clinical Oncology
https://www.readbyqxmd.com/read/28135215/muscular-strength-adaptations-and-hormonal-responses-after-two-different-multiple-set-protocols-of-resistance-training-in-postmenopausal-women
#13
Paulo Ricardo P Nunes, Larissa C Barcelos, Anselmo A Oliveira, Roberto Furlanetto Júnior, Fernanda M Martins, Elizabete Ap M R Resende, Fábio L Orsatti
We studied the effects of two different resistance training (RT) multiple-set protocols (three and six sets) on muscle strength and basal hormones concentrations in postmenopausal women (PW). Thirty-four PW were randomly allocated into three groups: control (CT, n=12), low RT volume (LV = three sets for each exercise, n=10) and high RT volume (HV = six sets for each exercise, n=12). The LV and HV groups performed eight exercises of a total body RT protocol three times a week, at 70 % of one repetition maximum (1RM) for 16 weeks...
January 20, 2017: Journal of Strength and Conditioning Research
https://www.readbyqxmd.com/read/28128973/the-role-of-volume-load-in-strength-and-absolute-endurance-adaptations-in-adolescent-s-performing-high-or-low-load-resistance-training
#14
RANDOMIZED CONTROLLED TRIAL
James Steele, James P Fisher, Ari R Assunção, Martim Bottaro, Paulo Gentil
This study compared high- (HL) and low-load (LL) resistance training (RT) on strength, absolute endurance, volume-load, and their relationships in untrained adolescents. Thirty-three untrained adolescents of both sexes (males, n = 17; females, n = 16; 14 ± 1 years) were randomly assigned into either (i) HL (n = 17): performing 3 sets of 4-6 repetitions to momentary concentric failure; or (ii) LL (n = 16): performing 2 sets of 12-15 repetitions to momentary concentric failure. RT was performed for 2×/week for 9 weeks...
February 2017: Applied Physiology, Nutrition, and Metabolism, Physiologie Appliquée, Nutrition et Métabolisme
https://www.readbyqxmd.com/read/28125698/late-course-adaptive-adjustment-based-on-metabolic-tumor-volume-changes-during-radiotherapy-may-reduce-radiation-toxicity-in-patients-with-non-small-cell-lung-cancer
#15
Linlin Xiao, Ning Liu, Guifang Zhang, Hui Zhang, Song Gao, Zheng Fu, Suzhen Wang, Qingxi Yu, Jinming Yu, Shuanghu Yuan
To reduce the high risk of radiation toxicity and enhance the quality of life of patients with non-small cell lung cancer (NSCLC), we quantified the metabolic tumor volumes (MTVs) from baseline to the late-course of radiotherapy (RT) by fluorodeoxyglucose positron emission tomography computerized tomography (FDG PET-CT) and discussed the potential benefit of late-course adaptive plans rather than original plans by dose volume histogram (DVH) comparisons. Seventeen patients with stage II-III NSCLC who were treated with definitive conventionally fractionated RT were eligible for this prospective study...
2017: PloS One
https://www.readbyqxmd.com/read/28125665/deregulated-lncrnas-in-b-cells-from-patients-with-active-tuberculosis
#16
Yurong Fu, Xianqin Xu, Junfang Xue, Wenping Duan, Zhengjun Yi
Role of lncRNAs in human adaptive immune response to TB infection is largely unexplored. To address this issue, here we characterized lncRNA expression profile in primary human B cell response to TB infection using microarray assay. Several lncRNAs and mRNAs were chosen for RT-qPCR validation. Bioinformatics prediction was applied to delineate function of the deregulated mRNAs. We found that 844 lncRNAs and 597 mRNAs were differentially expressed between B cell samples from individuals with or without TB. KEGG pathway analysis for the deregulated mRNAs indicated a number of pathways, such as TB, TLR signaling pathway and antigen processing and presentation...
2017: PloS One
https://www.readbyqxmd.com/read/28118819/short-bowel-syndrome-results-in-increased-gene-expression-associated-with-proliferation-inflammation-bile-acid-synthesis-and-immune-system-activation-rna-sequencing-a-zebrafish-sbs-model
#17
Kathy A Schall, Matthew E Thornton, Mubina Isani, Kathleen A Holoyda, Xiaogang Hou, Ching-Ling Lien, Brendan H Grubbs, Tracy C Grikscheit
BACKGROUND: Much of the morbidity associated with short bowel syndrome (SBS) is attributed to effects of decreased enteral nutrition and administration of total parenteral nutrition (TPN). We hypothesized that acute SBS alone has significant effects on gene expression beyond epithelial proliferation, and tested this in a zebrafish SBS model. METHODS: In a model of SBS in zebrafish (laparotomy, proximal stoma, distal ligation, n = 29) or sham (laparotomy alone, n = 28) surgery, RNA-Seq was performed after 2 weeks...
January 25, 2017: BMC Genomics
https://www.readbyqxmd.com/read/28117678/mechanism-governing-human-kappa-opioid-receptor-expression-under-desferrioxamine-induced-hypoxic-mimic-condition-in-neuronal-nmb-cells
#18
Jennifer Babcock, Alberto Herrera, George Coricor, Christopher Karch, Alexander H Liu, Aida Rivera-Gines, Jane L Ko
Cellular adaptation to hypoxia is a protective mechanism for neurons and relevant to cancer. Treatment with desferrioxamine (DFO) to induce hypoxia reduced the viability of human neuronal NMB cells. Surviving/attached cells exhibited profound increases of expression of the human kappa-opioid receptor (hKOR) and hypoxia inducible factor-1α (HIF-1α). The functional relationship between hKOR and HIF-1α was investigated using RT-PCR, Western blot, luciferase reporter, mutagenesis, siRNA and receptor-ligand binding assays...
January 20, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28116724/isolation-of-mature-adipocytes-from-white-adipose-tissue-and-gene-expression-studies-by-real-time-quantitative-rt-pcr
#19
Aurelie Nguyen Dinh Cat, Ana M Briones
The study of adipose tissue and more specifically of adipocytes is considered pivotal for dissecting molecular mechanisms responsible for alterations in several organs and systems, including adipose tissue, not only in obesity but also in other diseases (hypertension, heart failure). Adipose tissue is a complex tissue composed of adipocytes and the stromal vascular fraction which includes a heterogeneous population of preadipocytes, blood cells, endothelial cells, and macrophages. In the present chapter, methods are detailed to generate purified mature adipocytes from white adipose tissue by using enzymatic digestion...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28115547/proteomic-and-mutant-analysis-of-the-co2-concentrating-mechanism-of-hydrothermal-vent-chemolithoautotroph-thiomicrospira-crunogena
#20
Mary Mangiapia, Terry-René W Brown, Dale Chaput, Edward Haller, Tara L Harmer, Zahra Hashemy, Ryan Keeley, Juliana Leonard, Paola Mancera, David Nicholson, Stanley Stevens, Pauline Wanjugi, Tania Zabinski, Chongle Pan, Kathleen M Scott
: Many autotrophic microorganisms are likely to adapt to scarcity in dissolved inorganic carbon (DIC; = CO2 + HCO3(-) + CO3(2-)) with CO2 concentrating mechanisms (CCM) which actively transport DIC across the cell membrane to facilitate carbon fixation. Surprisingly, DIC transport has only been well-studied among cyanobacteria and microalgae. Deep-sea vent gammaproteobacterial chemolithoautotroph Thiomicrospira crunogena has a low-DIC inducible CCM, though the mechanism for uptake is unclear, as homologs to cyanobacterial transporters are absent...
January 23, 2017: Journal of Bacteriology
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