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Adaptive RT

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https://www.readbyqxmd.com/read/28923603/unraveling-the-expression-of-genes-involved-in-the-biosynthesis-pathway-of-cardiolipin-and-phosphatidylethalonamine-in-salmonella-hadar-grown-under-static-magnetic-field-200%C3%A2-mt
#1
Ramla Ben Mouhoub, Ahlem Mansouri, Khadidja Aliliche, Hamida Beghalem, Ahmed Landoulsi, Alya El May
We aim in this work to evaluate the effect of static magnetic field 200 mT (SMF) on the expression of genes involved in the biosynthetic pathway of cardiolipin: g3pd, plsB, cdsA, pgsA, pgpA, cls and phosphatidylethanolamine: pssA and psd in Salmonella enterica subsp Enterica serovar Hadar. Bacteria were exposed to a SMF during 3, 6 and 9 h. RNA extraction was followed by Reverse Transcriptase Polymerase Chain Reaction RT-PCR. The relative quantification of mRNA expression levels using 16S rRNA doesn't change during the time exposure...
September 15, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/28919888/the-sporothrix-schenckii-gene-encoding-for-the-ribosomal-protein-l6-has-constitutive-and-stable-expression-and-works-as-an-endogenous-control-in-gene-expression-analysis
#2
Elías Trujillo-Esquivel, José A Martínez-Álvarez, Diana M Clavijo-Giraldo, Nahúm V Hernández, Alberto Flores-Martínez, Patricia Ponce-Noyola, Héctor M Mora-Montes
Sporothrix schenckii is one of the causative agents of sporotrichosis, a worldwide-distributed mycosis that affects humans and other mammals. The interest in basic and clinical features of this organism has significantly increased in the last years, yet little progress in molecular aspects has been reported. Gene expression analysis is a set of powerful tools that helps to assess the cell response to changes in the extracellular environment, the genetic networks controlling metabolic pathways, and the adaptation to different growth conditions...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28916650/differential-expression-of-mirnas-in-the-respiratory-tree-of-the-sea-cucumber-apostichopus-japonicus-under-hypoxia-stress
#3
Da Huo, Lina Sun, Xiaoni Li, Xiaoshang Ru, Shilin Liu, Libin Zhang, Lili Xing, Hongsheng Yang
Sea cucumber, an important economic species, has encountered high mortality since 2013 in northern China because of seasonal environmental stress such as hypoxia, high temperature, and low salinity. MicroRNAs (miRNAs) are important in regulating gene expression in marine organisms in response to environmental change. In the present study, high-throughput sequencing was used to investigate alterations in miRNA expression in the sea cucumber under different levels of dissolved oxygen (DO). Nine small RNA libraries were constructed from the sea cucumber respiratory trees...
September 15, 2017: G3: Genes—Genomes—Genetics
https://www.readbyqxmd.com/read/28892246/novel-hyperthermia-applicator-system-allows-adaptive-treatment-planning-preliminary-clinical-results-in-tumour-bearing-animals
#4
S Dressel, M-C Gosselin, M H Capstick, E Carrasco, M S Weyland, S Scheidegger, E Neufeld, N Kuster, S Bodis, C Rohrer Bley
Hyperthermia (HT) as an adjuvant to radiation therapy (RT) is a multimodality treatment method to enhance therapeutic efficacy in different tumours. High demands are placed on the hardware and treatment planning software to guarantee adequately planned and applied HT treatments. The aim of this prospective study was to determine the effectiveness and safety of the novel HT system in tumour-bearing dogs and cats in terms of local response and toxicity as well as to compare planned with actual achieved data during heating...
September 11, 2017: Veterinary and Comparative Oncology
https://www.readbyqxmd.com/read/28887063/the-50kda-metalloproteinase-tvmp50-is-a-zinc-mediated-trichomonas-vaginalis-virulence-factor
#5
Jonathan Puente-Rivera, José Luis Villalpando, Alma Villalobos-Osnaya, Laura Isabel Vázquez-Carrillo, Gloria León-Ávila, María Dolores Ponce-Regalado, César López-Camarillo, Jose Miguel Elizalde-Contreras, Eliel Ruiz-May, Rossana Arroyo, María Elizbeth Alvarez-Sánchez
Trichomonas vaginalis is a protozoan parasite that can adapt to the trichomonicidal Zn(2+) concentrations of the male urogenital tract microenvironment. This adaptation is mediated by molecular mechanisms, including proteinase expression, that are regulated by cations such as Zn(2+). Herein, we characterized the previously identified 50kDa metalloproteinase aminopeptidase P (M24 family) member TvMP50 as a new Zn(2+)-mediated parasite virulence factor. Quantitative RT-PCR and indirect immunofluorescence assays corroborated the positive regulation of both mp50 gene expression and native TvMP50 protein overexpression in the cytoplasm and secretion products of parasites grown in the presence of Zn(2+)...
September 5, 2017: Molecular and Biochemical Parasitology
https://www.readbyqxmd.com/read/28883044/beneficial-effects-of-exercise-training-in-heart-failure-are-lost-in-male-diabetic-rats
#6
Dalila Boudia, Valérie Domergue, Phlippe Mateo, Loubina Fazal, Mathilde Prud'homme, Heloise Prigent, Claude Delcayre, Alain Cohen-Solal, Anne Garnier, Renee Ventura-Clapier, Jane-Lise Samuel
Exercise training has been demonstrated to have beneficial effects in patients with heart failure (HF) or diabetes. However, it is unknown whether diabetic patients with HF will benefit from exercise training. Male Wistar rats were fed either a standard (Sham, n= 53) or high-fat, high-sucrose (HFHS) diet (D, n=66) for 6 months. After 2 months of diet, the rats were already diabetic. Rats were then randomly subjected to either myocardial infarction by coronary artery ligation (MI) or sham operation (Sham). Two months later, heart failure was documented by echocardiography and animals were randomly subjected to exercise training with treadmill for eight additional weeks or remained sedentary...
September 7, 2017: Journal of Applied Physiology
https://www.readbyqxmd.com/read/28875538/a-generalized-parametric-response-mapping-method-for-analysis-of-multi-parametric-imaging-a-feasibility-study-with-application-to-glioblastoma
#7
Anthony Lausch, Timothy Pok-Chi Yeung, Jeff Chen, Elton Law, Yong Wang, Benedetta Urbini, Filippo Donelli, Luigi Manco, Enrico Fainardi, Ting-Yim Lee, Eugene Wong
PURPOSE: Parametric response map (PRM) analysis of functional imaging has been shown to be an effective tool for early prediction of cancer treatment outcomes and may also be well-suited towards guiding personalized adaptive radiotherapy (RT) strategies such as sub-volume boosting. However, the PRM method was primarily designed for analysis of longitudinally acquired pairs of single-parameter image data. The purpose of this study was to demonstrate the feasibility of a generalized parametric response map analysis framework which enables analysis of multi-parametric data while maintaining the key advantages of the original PRM method...
September 6, 2017: Medical Physics
https://www.readbyqxmd.com/read/28869163/biologically-adapted-radiation-therapy
#8
Daniela Thorwarth
The aim of biologically adapted radiotherapy (RT) is to shape or paint the prescribed radiation dose according to biological properties of the tumor in order to increase local control rates in the future. Human tumors are known to present with an extremely heterogeneous tissue architecture leading to highly variable local cell densities and chaotic vascular structures leading to tumor hypoxia and regions of increased radiation resistance. The goal of biologically adapted RT or dose painting is to individually adapt the radiation dose to biological features of the tumor as non-invasively assessed with functional imaging in order to overcome increased radiation resistance...
August 28, 2017: Zeitschrift Für Medizinische Physik
https://www.readbyqxmd.com/read/28868253/relationships-between-regional-radiation-doses-and-cognitive-decline-in-children-treated-with-cranio-spinal-irradiation-for-posterior-fossa-tumors
#9
Elodie Doger de Speville, Charlotte Robert, Martin Perez-Guevara, Antoine Grigis, Stephanie Bolle, Clemence Pinaud, Christelle Dufour, Anne Beaudré, Virginie Kieffer, Audrey Longaud, Jacques Grill, Dominique Valteau-Couanet, Eric Deutsch, Dimitri Lefkopoulos, Catherine Chiron, Lucie Hertz-Pannier, Marion Noulhiane
Pediatric posterior fossa tumor (PFT) survivors who have been treated with cranial radiation therapy often suffer from cognitive impairments that might relate to IQ decline. Radiotherapy (RT) distinctly affects brain regions involved in different cognitive functions. However, the relative contribution of regional irradiation to the different cognitive impairments still remains unclear. We investigated the relationships between the changes in different cognitive scores and radiation dose distribution in 30 children treated for a PFT...
2017: Frontiers in Oncology
https://www.readbyqxmd.com/read/28865518/radiation-induced-liver-toxicity
#10
REVIEW
Pablo Munoz-Schuffenegger, Sylvia Ng, Laura A Dawson
The advent of highly conformal radiation therapy (RT) has defined a new role for RT in the treatment of both primary and metastatic liver cancer. Despite major advances in how RT is delivered, radiation-induced liver disease (RILD) remains a concern. Classic RILD, characterized by anicteric ascites and hepatomegaly, is unlikely to occur if treating to doses of ≤30Gy in 2Gy per fraction in patients with baseline Child-Pugh A liver function. On the other hand, nonclassic RILD is a spectrum of liver toxicity, including a general decline in liver function and elevation of liver enzymes...
October 2017: Seminars in Radiation Oncology
https://www.readbyqxmd.com/read/28861791/rt-quic-assays-for-prion-disease-detection-and-diagnostics
#11
Christina D Orrù, Bradley R Groveman, Andrew G Hughson, Matteo Manca, Lynne D Raymond, Gregory J Raymond, Katrina J Campbell, Kelsie J Anson, Allison Kraus, Byron Caughey
In coping with prion diseases, it is important to have tests that are practical enough for routine applications in medicine, agriculture, wildlife biology, and research, yet sensitive enough to detect minimal amounts of infectivity. Real-time quaking-induced conversion (RT-QuIC) assays have evolved to the point where they fulfill these criteria in applications to various human and animal prion diseases. For example, RT-QuIC assays of cerebrospinal fluid and nasal brushings allow for highly sensitive (77-97%) and specific (99-100%) identification of human sCJD patients...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28861748/physiological-and-molecular-responses-of-seedlings-of-an-upland-rice-tung-lu-3-to-total-submergence-compared-to-those-of-a-submergence-tolerant-lowland-rice-fr13a
#12
Si-Yu Yang, Yu-Sian Wu, Chung-Tse Chen, Ming-Hsin Lai, Hsing-Mu Yen, Chin-Ying Yang
BACKGROUND: Understanding the responses of rice to environmental stresses such as unscheduled submergence is of pressing important owing to increasing severity of weather thought to arise from global climate change. When rice is completely submerged, different types adopt either a quiescence survival strategy (i.e., minimal shoot elongation) or an escape strategy (i.e., enhanced shoot elongation). Each strategy can prolong survival depending on the circumstances. While submergence responses have been studied in rice typical of lowland and flood-prone areas, few studies have explored the physiological and molecular properties of upland rice under submergence...
August 31, 2017: Rice
https://www.readbyqxmd.com/read/28861706/xerostomia-quality-of-life-scale-xeqols-questionnaire-validation-of-italian-version-in-head-and-neck-cancer-patients
#13
Luciana Lastrucci, Silvia Bertocci, Vittorio Bini, Simona Borghesi, Roberta De Majo, Andrea Rampini, Pietro Giovanni Gennari, Paola Pernici
AIM: To translate the Xerostomia Quality-of-Life Scale (XeQoLS) into Italian language (XeQoLS-IT). Xerostomia is the most relevant acute and late toxicity in patients with head and neck cancer treated with radiotherapy (RT). Patient-reported outcome (PRO) instruments are subjective report on patient perception of health status. The XeQoLS consists of 15 items and measures the impact of salivary gland dysfunction and xerostomia on the four major domains of oral health-related QoL. METHODS: The XeQoLS-IT was created through a linguistic validation multi-step process: forward translation (TF), backward translation (TB) and administration of the questionnaire to 35 Italian patients with head and neck cancer...
August 31, 2017: La Radiologia Medica
https://www.readbyqxmd.com/read/28855912/salt-sensitive-signaling-networks-in-the-mediation-of-k-na-homeostasis-gene-expression-in-glycyrrhiza-uralensis-roots
#14
Tao Lang, Shurong Deng, Nan Zhao, Chen Deng, Yinan Zhang, Yanli Zhang, Huilong Zhang, Gang Sa, Jun Yao, Caiwu Wu, Yanhong Wu, Qun Deng, Shanzhi Lin, Jianxin Xia, Shaoliang Chen
We investigated the effects of salt-sensitive signaling molecules on ionic fluxes and gene expression related to K(+)/Na(+) homeostasis in a perennial herb, Glycyrrhiza uralensis, during short-term NaCl stress (100 mM, 24 h). Salt treatment caused more pronounced Na(+) accumulation in root cells than in leaf cells. Na(+) ions were mostly compartmentalized in vacuoles. Roots exposed to NaCl showed increased levels of extracellular ATP (eATP), cytosolic Ca(2+), H2O2, and NO. Steady-state flux recordings revealed that these salt-sensitive signaling molecules enhanced NaCl-responsive Na(+) efflux, due to the activated Na(+)/H(+) antiport system in the plasma membrane (PM)...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28846563/temporal-response-of-angiogenesis-and-hypertrophy-to-resistance-training-in-young-men
#15
Tanya M Holloway, Tim Snijders, Janneau van Kranenburg, Luc Jc van Loon, Lex B Verdijk
While endurance exercise training promotes angiogenesis and improves metabolic health, the effect of resistance training on this process is less well defined. We hypothesised that capillarization would increase proportionally, and concurrently, with muscle fiber hypertrophy in response to resistance training in young men. METHODS: In this double blind, randomized control trial, 36 males (22 ± 1 y) were randomized to placebo or protein supplementation, and participated in 12-wk of resistance training...
August 25, 2017: Medicine and Science in Sports and Exercise
https://www.readbyqxmd.com/read/28844966/lipopolysaccharide-specific-binding-c-type-lectin-with-one-crd-domain-from-fenneropenaeus-merguiensis-fmlc4-functions-as-a-pattern-recognition-receptor-in-shrimp-innate-immunity
#16
Prapaporn Utarabhand, Supattra Thepnarong, Phanthipha Runsaeng
In crustaceans, an innate immune system is solely required because they lack an adaptive immunity. One kind of pattern recognition receptors (PRRs) that plays a particular role in the innate immunity of aquatic shrimp is lectin. A new diverse C-type lectin (FmLC4) was cloned from the hepatopancreas of Fenneropenaeus merguiensis by using RT-PCR and 5' and 3' rapid amplification of cDNA ends approaches. A full-length FmLC4 cDNA comprises 706 bp with an open reading frame of 552 bp, encoding a peptide of 184 amino acids...
August 24, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28839258/birc3-is-a-biomarker-of-mesenchymal-habitat-of-glioblastoma-and-a-mediator-of-survival-adaptation-in-hypoxia-driven-glioblastoma-habitats
#17
Dapeng Wang, Anders E Berglund, Rajappa S Kenchappa, Robert J MacAulay, James J Mulé, Arnold B Etame
Tumor hypoxia is an established facilitator of survival adaptation and mesenchymal transformation in glioblastoma (GBM). The underlying mechanisms that direct hypoxia-mediated survival in GBM habitats are unclear. We previously identified BIRC3 as a mediator of therapeutic resistance in GBM to standard temozolomide (TMZ) chemotherapy and radiotherapy (RT). Here we report that BIRC3 is a biomarker of the hypoxia-mediated adaptive mesenchymal phenotype of GBM. Specifically, in the TCGA dataset elevated BIRC3 gene expression was identified as a superior and selective biomarker of mesenchymal GBM versus neural, proneural and classical subtypes...
August 24, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28835206/an-optimised-protocol-for-isolation-of-rna-from-small-sections-of-laser-capture-microdissected-ffpe-tissue-amenable-for-next-generation-sequencing
#18
Parisa Amini, Julia Ettlin, Lennart Opitz, Elena Clementi, Alexandra Malbon, Enni Markkanen
BACKGROUND: Formalin-fixed paraffin embedded (FFPE) tissue constitutes a vast treasury of samples for biomedical research. Thus far however, extraction of RNA from FFPE tissue has proved challenging due to chemical RNA-protein crosslinking and RNA fragmentation, both of which heavily impact on RNA quantity and quality for downstream analysis. With very small sample sizes, e.g. when performing Laser-capture microdissection (LCM) to isolate specific subpopulations of cells, recovery of sufficient RNA for analysis with reverse-transcription quantitative PCR (RT-qPCR) or next-generation sequencing (NGS) becomes very cumbersome and difficult...
August 23, 2017: BMC Molecular Biology
https://www.readbyqxmd.com/read/28831052/rapid-amplification-of-four-retrotransposon-families-promoted-speciation-and-genome-size-expansion-in-the-genus-panax
#19
Junki Lee, Nomar Espinosa Waminal, Hong-Il Choi, Sampath Perumal, Sang-Choon Lee, Van Binh Nguyen, Woojong Jang, Nam-Hoon Kim, Li-Zhi Gao, Tae-Jin Yang
Genome duplication and repeat multiplication contribute to genome evolution in plants. Our previous work identified a recent allotetraploidization event and five high-copy LTR retrotransposon (LTR-RT) families PgDel, PgTat, PgAthila, PgTork, and PgOryco in Panax ginseng. Here, using whole-genome sequences, we quantified major repeats in five Panax species and investigated their role in genome evolution. The diploids P. japonicus, P. vietnamensis, and P. notoginseng and the tetraploids P. ginseng and P. quinquefolius were analyzed alongside their relative Aralia elata...
August 22, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28819168/resistance-training-regulates-gene-expression-of-molecules-associated-with-intramyocellular-lipids-glucose-signaling-and-fiber-size-in-old-rats
#20
Manoel Benício Teixeira Ribeiro, Vinicius Guzzoni, Jeffrey M Hord, Gisele Nunes Lopes, Rita de Cássia Marqueti, Rosângela Vieira de Andrade, Heloisa Sobreiro Selistre-de-Araujo, João Luiz Q Durigan
Sarcopenia is a complex multifactorial process, some of which involves fat infiltration. Intramyocellular lipid (IMCL) accumulation is postulated to play a role on sarcopenia during aging, which is believed to be due alterations in glucose homeostasis in the skeletal muscle. Sarcopenia, along with intramuscular lipids, is associated with physical inactivity. Resistance training (RT) has been indicated to minimize the age-induced muscle skeletal adaptations. Thus, we aimed to investigate the effects of RT on mRNA levels of regulatory components related to intramyocellular lipid, glucose metabolism and fiber size in soleus and gastrocnemius muscles of aged rats...
August 17, 2017: Scientific Reports
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