keyword
MENU ▼
Read by QxMD icon Read
search

bacterial lysate

keyword
https://www.readbyqxmd.com/read/29036687/measuring-deaminated-nucleotide-surveillance-enzyme-itpa-activity-with-an-atp-releasing-nucleotide-chimera
#1
Debin Ji, Elena I Stepchenkova, Jian Cui, Miriam R Menezes, Youri I Pavlov, Eric T Kool
Nucleotide quality surveillance enzymes play important roles in human health, by detecting damaged molecules in the nucleotide pool and deactivating them before they are incorporated into chromosomal DNA or adversely affect metabolism. In particular, deamination of adenine moiety in (deoxy)nucleoside triphosphates, resulting in formation of (d)ITP, can be deleterious, leading to DNA damage, mutagenesis and other harmful cellular effects. The 21.5 kDa human enzyme that mitigates this damage by conversion of (d)ITP to monophosphate, ITPA, has been proposed as a possible therapeutic and diagnostic target for multiple diseases...
September 28, 2017: Nucleic Acids Research
https://www.readbyqxmd.com/read/28916188/mast-cell-chymase-decreases-the-severity-of-group-b-streptococcus-infections
#2
Claire Gendrin, Nicholas J Shubin, Erica Boldenow, Sean Merillat, Morgan Clauson, Danial Power, Kelly S Doran, Magnus Abrink, Gunnar Pejler, Lakshmi Rajagopal, Adrian M Piliponsky
BACKGROUND: Group B Streptococcus (GBS) or Streptococcus agalactiae are β-hemolytic gram-positive bacteria that colonize the lower genital tracts of women and are frequently associated with infections during pregnancy. Innate immune defenses are critical for controlling GBS dissemination and systemic infection. Mast cells are resident sentinel cells that come into contact with pathogens early during colonization and infection. OBJECTIVE: We aimed to investigate the contribution of chymase to systemic GBS infection and rates of preterm birth...
September 12, 2017: Journal of Allergy and Clinical Immunology
https://www.readbyqxmd.com/read/28904643/rolling-circle-mutagenesis-of-gst-mcherry-to-understand-mutation-gene-expression-and-regulation
#3
Jessica Cole, Amanda Ferguson, Verónica A Segarra, Susan Walsh
Undergraduates are often familiar with textbook examples of human mutations that affect coding regions and the subsequent disorders, but they may struggle with understanding the implications of mutations in the regulatory regions of genes. We have designed a laboratory sequence that will allow students to explore the effect random mutagenesis can have on protein function, expression, and ultimately phenotype. Students design and perform a safe and time-efficient random mutagenesis experiment using error-prone rolling circular amplification of a plasmid expressing the inducible fusion protein glutathione S-transferase (GST)-mCherry...
2017: Journal of Microbiology & Biology Education: JMBE
https://www.readbyqxmd.com/read/28877283/bacterial-endotoxin-adhesion-to-different-types-of-orthodontic-adhesives
#4
COMPARATIVE STUDY
Priscilla Coutinho Romualdo, Thaís Rodrigues Guerra, Fábio Lourenço Romano, Raquel Assed Bezerra da Silva, Izaíra Tincani Brandão, Célio Lopes Silva, Lea Assed Bezerra da Silva, Paulo Nelson-Filho
Objective: The aim of this study was to assess whether LPS adheres to orthodontic adhesive systems, comparing two commercial brands. Material and Methods: Forty specimens were fabricated from Transbond XT and Light Bond composite and bonding agent components (n=10/component), then contaminated by immersion in a bacterial endotoxin solution. Contaminated and non-contaminated acrylic resin samples were used as positive and negative control groups, respectively. LPS quantification was performed by the Limulus Amebocyte Lysate QCL-1000™ test...
July 2017: Journal of Applied Oral Science: Revista FOB
https://www.readbyqxmd.com/read/28869736/-expression-purification-stability-and-transduction-efficiency-of-full-length-sod2-recombinant-proteins
#5
Jianru Pan, Lijuan Chen, Huocong He, Ying Su, Xiangling Wang, Xian Li, Cuihuang Chen, Lunqiao Wu, Shutao Liu
Superoxide dismutase (SOD) family is necessary to protect cells from the toxicity of reactive oxygen species produced during normal metabolism. Among SODs, manganese-containing superoxide dismutase (Mn-SOD, SOD2) is the most important one. The DNA fragment containing the full nucleotide of full-length human SOD2 was synthesized and inserted into the prokaryotic expression vector pGEX-4T-1 with tag GST. DNA construct was then transformed into Escherichia coli BL21 (DE3) and expression was induced with IPTG at 25 ℃...
July 25, 2017: Sheng Wu Gong Cheng Xue Bao, Chinese Journal of Biotechnology
https://www.readbyqxmd.com/read/28847096/evaluation-of-water-quality-during-successive-severe-drought-years-within-microcystis-blooms-using-fish-embryo-toxicity-tests-for-the-san-francisco-estuary-california
#6
Tomofumi Kurobe, Peggy W Lehman, M E Haque, Tiziana Sedda, Sarah Lesmeister, Swee Teh
In the San Francisco Estuary, California, the largest estuary on the Pacific Coast of North America, the frequency and intensity of drought and associated cyanobacteria blooms are predicted to increase with climate change. To assess the impact of water quality conditions on estuarine fish health during successive severe drought years with Microcystis blooms, we performed fish embryo toxicity testing with Delta Smelt and Medaka. Fish embryos were exposed to filtered ambient water collected from the San Francisco Estuary during the Microcystis bloom season in 2014 and 2015, the third and fourth most severe recorded drought years in California...
January 1, 2018: Science of the Total Environment
https://www.readbyqxmd.com/read/28836022/covalent-display-of-an-active-chimeric-recombinant-tissue-plasminogen-activator-on-polyhydroxybutyrate-granules-surface
#7
Akram Hafizi, Mohamad Ali Malboobi, Mokhtar Jalali-Javaran, Pal Maliga, Houshang Alizadeh
OBJECTIVE: To develop a deliberately engineered expression and purification system for an active chimeric-recombinant tissue plasminogen activator (crtPA) using co-expression with polyhydroxybutyrate (PHB) operon genes. RESULTS: Fusion of crtPA with PhaC-synthase simplified the purification steps through crtPA sedimentation with PHB particles. Moreover, the covalently immobilized crtPA was biologically active as shown in a chromogenic assay. Upon WELQut-protease activity, the released single-chain crtPA converted to the two-chain form which produced a pattern of bands with approx...
August 23, 2017: Biotechnology Letters
https://www.readbyqxmd.com/read/28808331/pro-and-anti-inflammatory-responses-of-peripheral-blood-mononuclear-cells-induced-by-staphylococcus-aureus-and-pseudomonas-aeruginosa-phages
#8
Jonas D Van Belleghem, Frédéric Clement, Maya Merabishvili, Rob Lavigne, Mario Vaneechoutte
The ability of bacteriophages to kill bacteria is well known, as is their potential use as alternatives to antibiotics. As such, bacteriophages reach high doses locally through infection of their bacterial host in the human body. In this study we assessed the gene expression profile of peripheral blood monocytes from six donors for twelve immunity-related genes (i.e. CD14, CXCL1, CXCL5, IL1A, IL1B, IL1RN, IL6, IL10, LYZ, SOCS3, TGFBI and TNFA) induced by Staphylococcus aureus phage ISP and four Pseudomonas aeruginosa phages (i...
August 14, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28806642/circulating-follicular-helper-t-cells-presented-distinctively-different-responses-toward-bacterial-antigens-in-primary-biliary-cholangitis
#9
Zun-Qiang Zhou, Da-Nian Tong, Jiao Guan, Mei-Fang Li, Qi-Ming Feng, Min-Jie Zhou, Zheng-Yun Zhang
Primary biliary cholangitis (PBC) is a chronic and progressive cholestatic liver disease with unknown causes. The initiation of PBC is associated with bacterial infections and abnormal immune correlates, such as the presence of self-reactive anti-mitochondrial antibodies and shifted balance of T cell subsets. In particular, the CD4(+)CXCR5(+) follicular helper T (Tfh) cells are highly activated in PBC patients and are significantly associated with PBC severity, but the underlying reasons are unknown. In this study, we found that the circulating CD4(+)CXCR5(+) T cells were enriched with the interferon (IFN)-γ-secreting Th1-subtype and the interleukin (IL)-17-secreting Th17-subtype, but not the IL-4-secreting Th2 subtype...
August 11, 2017: International Immunopharmacology
https://www.readbyqxmd.com/read/28802077/helicobacter-pylori-and-its-secreted-immunomodulator-vaca-protect-against-anaphylaxis-in-experimental-models-of-food-allergy
#10
A Kyburz, S Urban, A Altobelli, S Floess, J Huehn, T L Cover, A Müller
BACKGROUND: Food allergy is an increasingly common health problem in Western populations. Epidemiological studies have suggested both positive and negative associations between food allergy and infection with the gastric bacterium Helicobacter pylori. OBJECTIVE: The objective of this work was to investigate whether experimental infection with H. pylori, or prophylactic treatment with H. pylori-derived immunomodulatory molecules, affects the onset and severity of food allergy, either positively or negatively...
October 2017: Clinical and Experimental Allergy: Journal of the British Society for Allergy and Clinical Immunology
https://www.readbyqxmd.com/read/28801725/optimization-of-soluble-expression-and-purification-of-recombinant-human-rhinovirus-type-14-3c-protease-using-statistically-designed-experiments-isolation-and-characterization-of-the-enzyme
#11
Georgia Antoniou, Irineos Papakyriacou, Christos Papaneophytou
Human rhinovirus (HRV) 3C protease is widely used in recombinant protein production for various applications such as biochemical characterization and structural biology projects to separate recombinant fusion proteins from their affinity tags in order to prevent interference between these tags and the target proteins. Herein, we report the optimization of expression and purification conditions of glutathione S-transferase (GST)-tagged HRV 3C protease by statistically designed experiments. Soluble expression of GST-HRV 3C protease was initially optimized by response surface methodology (RSM), and a 5...
August 11, 2017: Molecular Biotechnology
https://www.readbyqxmd.com/read/28800332/cerebral-oxidative-stress-and-microvasculature-defects-in-tnf-%C3%AE-expressing-transgenic-and-porphyromonas-gingivalis-infected-apoe-mice
#12
Farheen Rokad, Ryan Moseley, Rowan S Hardy, Sasanka Chukkapalli, StJohn Crean, Lakshmyya Kesavalu, Sim K Singhrao
The polymicrobial dysbiotic subgingival biofilm microbes associated with periodontal disease appear to contribute to developing pathologies in distal body sites, including the brain. This study examined oxidative stress, in the form of increased protein carbonylation and oxidative protein damage, in the tumor necrosis factor-α (TNF-α) transgenic mouse that models inflammatory TNF-α excess during bacterial infection; and in the apolipoprotein knockout (ApoE-/-) mouse brains, following Porphyromonas gingivalis gingival monoinfection...
2017: Journal of Alzheimer's Disease: JAD
https://www.readbyqxmd.com/read/28795570/rapid-and-scalable-preparation-of-bacterial-lysates-for-cell-free-gene-expression
#13
Andriy Didovyk, Taishi Tonooka, Lev Tsimring, Jeff Hasty
Cell-free gene expression systems are emerging as an important platform for a diverse range of synthetic biology and biotechnology applications, including production of robust field-ready biosensors. Here, we combine programmed cellular autolysis with a freeze-thaw or freeze-dry cycle to create a practical, reproducible, and a labor- and cost-effective approach for rapid production of bacterial lysates for cell-free gene expression. Using this method, robust and highly active bacterial cell lysates can be produced without specialized equipment at a wide range of scales, making cell-free gene expression easily and broadly accessible...
August 21, 2017: ACS Synthetic Biology
https://www.readbyqxmd.com/read/28777328/direct-identification-of-functional-amyloid-proteins-by-label-free-quantitative-mass-spectrometry
#14
Heidi N Danielsen, Susan H Hansen, Florian-Alexander Herbst, Henrik Kjeldal, Allan Stensballe, Per H Nielsen, Morten S Dueholm
Functional amyloids are important structural and functional components of many biofilms, yet our knowledge of these fascinating polymers is limited to a few examples for which the native amyloids have been isolated in pure form. Isolation of the functional amyloids from other cell components represents a major bottleneck in the search for new functional amyloid systems. Here we present a label-free quantitative mass spectrometry method that allows identification of amyloid proteins directly in cell lysates...
August 4, 2017: Biomolecules
https://www.readbyqxmd.com/read/28770946/analysis-of-the-effect-of-bacterial-lysate-and-the-immunologic-mechanism-in-treating-infant-bronchiolitis
#15
Y-W Liu, S-H Dong, G-Y Zhan, H-Z Tan, Y-Q Peng, F Wei
OBJECTIVE: We studied the effect of bacterial lysate and the immunologic mechanism in treating infant bronchiolitis. PATIENTS AND METHODS: 124 infants were diagnosed with bronchiolitis were consecutively selected and randomly divided into control group and observation group, with 62 cases in each group. Conventional therapies were administered in the control group, while bacterial lysates were administered in the observation group. Therapeutic effects were compared after 14 days...
July 2017: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/28752459/expression-and-purification-of-active-recombinant-human-alpha-1-antitrypsin-aat-from-escherichia-coli
#16
Beena Krishnan, Lizbeth Hedstrom, Daniel N Hebert, Lila M Gierasch, Anne Gershenson
Well-established genetic manipulation procedures along with a fast doubling time, the ability to grow in inexpensive media, and easy scaleup make Escherichia coli (E. coli) a preferred recombinant protein expression platform. Human alpha-1 antitrypsin (AAT) and other serpins are easily expressed in E. coli despite their metastability and complicated topology. Serpins can be produced as soluble proteins or aggregates in inclusion bodies, and both forms can be purified to homogeneity. In this chapter, we describe an ion-exchange chromatography-based protocol that we have developed involving the use of two anion-exchange columns to purify untagged human AAT from E...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28746806/disposable-amperometric-polymerase-chain-reaction-free-biosensor-for-direct-detection-of-adulteration-with-horsemeat-in-raw-lysates-targeting-mitochondrial-dna
#17
Víctor Ruiz-Valdepeñas Montiel, María L Gutiérrez, Rebeca M Torrente-Rodríguez, Eloy Povedano, Eva Vargas, Á Julio Reviejo, Rosario Linacero, Francisco J Gallego, Susana Campuzano, José M Pingarrón
A novel electrochemical disposable nucleic acid biosensor for simple, rapid, and specific detection of adulterations with horsemeat is reported in this work. The biosensing platform involves immobilization of a 40-mer RNA probe specific for a characteristic fragment of the mitochondrial DNA D-loop region of horse onto the surface of magnetic microcarriers. In addition, signal amplification was accomplished by using a commercial antibody specific to RNA/DNA duplexes and a bacterial protein conjugated with a horseradish peroxidase homopolymer (ProtA-HRP40)...
September 5, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28744452/secretory-products-of-the-human-gi-tract-microbiome-and-their-potential-impact-on-alzheimer-s-disease-ad-detection-of-lipopolysaccharide-lps-in-ad-hippocampus
#18
Yuhai Zhao, Vivian Jaber, Walter J Lukiw
Although the potential contribution of the human gastrointestinal (GI) tract microbiome to human health, aging, and disease is becoming increasingly acknowledged, the molecular mechanics and signaling pathways of just how this is accomplished is not well-understood. Major bacterial species of the GI tract, such as the abundant Gram-negative bacilli Bacteroides fragilis (B. fragilis) and Escherichia coli (E. coli), secrete a remarkably complex array of pro-inflammatory neurotoxins which, when released from the confines of the healthy GI tract, are pathogenic and highly detrimental to the homeostatic function of neurons in the central nervous system (CNS)...
2017: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/28736134/stimulus-evoked-erk-dependent-phosphorylation-of-activity-regulated-cytoskeleton-associated-protein-arc-regulates-its-neuronal-subcellular-localization
#19
Oleksii Nikolaienko, Maria Steene Eriksen, Sudarshan Patil, Haruhiko Bito, Clive R Bramham
Activity-regulated cytoskeletal-associated protein (Arc) is implicated as a master regulator of long-term synaptic plasticity and memory formation in mammalian brain. Arc acts at synapses and within the nucleus, but the mechanisms controlling Arc localization and function are little known. As Arc transcription and translation are regulated by extracellularsignal-regulated kinase (ERK) signaling, we asked whether Arc protein itself is phosphorylated by ERK. GST-fused Arc of rat origin was able to pull down endogenous ERK2 from rat hippocampal lysates...
July 21, 2017: Neuroscience
https://www.readbyqxmd.com/read/28715499/helicobacter-pylori-modulates-host-cell-responses-by-cagt4ss-dependent-translocation-of-an-intermediate-metabolite-of-lps-inner-core-heptose-biosynthesis
#20
Saskia C Stein, Eugenia Faber, Simon H Bats, Tatiana Murillo, Yvonne Speidel, Nina Coombs, Christine Josenhans
Highly virulent Helicobacter pylori cause proinflammatory signaling inducing the transcriptional activation and secretion of cytokines such as IL-8 in epithelial cells. Responsible in part for this signaling is the cag pathogenicity island (cagPAI) that codetermines the risk for pathological sequelae of an H. pylori infection such as gastric cancer. The Cag type IV secretion system (CagT4SS), encoded on the cagPAI, can translocate various molecules into cells, the effector protein CagA, peptidoglycan metabolites and DNA...
July 2017: PLoS Pathogens
keyword
keyword
73996
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"