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https://www.readbyqxmd.com/read/28318451/bacterial-lysate-for-the-prevention-of-chronic-rhinosinusitis-recurrence-in-children
#1
J Chen, Y Zhou, J Nie, Y Wang, L Zhang, Q Shi, H Tan, W Kong
BACKGROUND: Chronic rhinosinusitis is a common nasal disorder in children that is prone to recurrence. This study investigated the prevention of chronic rhinosinusitis recurrence with bacteria lysate in children. METHODS: Bacteria lysate was administered 10 days per month for 3 months to children with chronic rhinosinusitis, who had just entered a remission phase. Visual analogue score, nasal symptoms scores, rhinitis attack frequency and antibiotic use were assessed at three months and one year...
March 20, 2017: Journal of Laryngology and Otology
https://www.readbyqxmd.com/read/28315252/profiling-of-protease-cleavage-sites-by-proteome-derived-peptide-libraries-and-quantitative-proteomics
#2
Chia-Yi Chen, Bettina Mayer, Oliver Schilling
Biochemical profiling of active site specificity is a crucial step to characterize proteases, which play key roles in health and disease. Here, we present a protocol using proteome-derived peptide libraries in combination with quantitative proteomics to simultaneously identify cleavage motifs N- and C-terminal to the scissile peptide bond. First, bacterial or eukaryotic cell lysate is used to generate peptide libraries. Without further chemical modification, peptide libraries are then split into control and treated (incubate with active protease) aliquots...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28298196/microbial-detoxification-of-eleven-food-and-feed-contaminating-trichothecene-mycotoxins
#3
Rafiq Ahad, Ting Zhou, Dion Lepp, K Peter Pauls
BACKGROUND: Contamination of agricultural commodities with multiple trichothecene mycotoxins, produced by toxigenic Fusarium species, is a food safety issue, which greatly affects grain production and marketing worldwide. Importantly, exposure to multiple trichothecenes may increase toxicity in animals due to their synergistic and/or additive effects. To address the problem this study aimed to achieve a novel biological trait capable of detoxifying various food and feed contaminating trichothecenes under aerobic and anaerobic conditions and wide range of temperatures...
March 15, 2017: BMC Biotechnology
https://www.readbyqxmd.com/read/28284007/erratum-to-protein-protein-interaction-analysis-in-crude-bacterial-lysates-using-combinational-method-of-19-f-site-specific-incorporation-and-19-f-nmr
#4
Dong Li, Yanan Zhang, Yao He, Chengwei Zhang, Jiefei Wang, Ying Xiong, Longhua Zhang, Yangzhong Liu, Pan Shi, Changlin Tian
No abstract text is available yet for this article.
March 10, 2017: Protein & Cell
https://www.readbyqxmd.com/read/28258293/interaction-between-methicillin-resistant-staphylococcus-aureus-mrsa-and-acanthamoeba-polyphaga
#5
Thamires Klein de Souza, Scheila Silva Soares, Lisianne Brittes Benitez, Marilise Brittes Rott
The interactions that occur between bacteria and amoebae can give through mutual relations, where both organisms benefit from the association or parasitic in which one organism benefits at the expense of the other. When these organisms share the same environment, it can result in some changes in the growth of organisms, in adaptation patterns, in morphology, development or even in their ability to synthesize proteins and other substances. In this study, the interaction between Acanthamoeba polyphaga and Staphylococcus aureus (MRSA) was evaluated using a co-culture model at different incubation times...
March 3, 2017: Current Microbiology
https://www.readbyqxmd.com/read/28238861/comparative-analysis-of-immunocompetence-between-females-and-males-in-the-sea-cucumber-apostichopus-japonicus
#6
Jingwei Jiang, Zunchun Zhou, Ying Dong, Shan Gao, Hongjuan Sun, Zhong Chen, Aifu Yang, Hesheng Su
In order to preliminarily understand the immune difference between females and males in the sea cucumber Apostichopus japonicus, the activities assay of superoxide dismutase (SOD), catalase (CAT), peroxidase (POD), phenoloxidase (PO), acid phosphatase (ACP) and alkaline phosphatase (ALP) with biochemical methods, the detection of PO isozymes with native-PAGE and catechol staining, and the test of antibacterial activities with bacterial growth curve determination method were performed in this study using cell-free coelomic fluid (CCF) and coelomocyte lysate supernatant (CLS) from females and males as the samples...
February 24, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28215401/nickel-salen-supported-paramagnetic-nanoparticles-for-6-his-target-recombinant-protein-affinity-purification
#7
Zahra Rashid, Ramin Ghahremanzadeh, Mohammad-Reza Nejadmoghaddam, Mahboobeh Nazari, Mohammad-Reza Shokri, Hossein Naeimi, Amir-Hassan Zarnani
In this research, a simple, efficient, inexpensive, rapid and high yield method for the purification of 6×histidine-tagged recombinant protein was developed. For this purpose, manganese ferrite magnetic nanoparticles (MNPs) were synthesized through a co-precipitation method and then they were conveniently surface-modified with tetraethyl orthosilicate (TEOS) in order to prevent oxidation and form high density of hydroxyl groups. Next, the salen ligand was prepared from condensation reaction of salicylaldehyde and 3-aminopropyl (trimethoxy) silane (APTMS) in 1:1 molar ratio; followed by complexation with Ni(OAc)2...
February 13, 2017: Journal of Chromatography. A
https://www.readbyqxmd.com/read/28214364/in-situ-analysis-of-bacillus-licheniformis-biofilms-amyloid-like-polymers-and-edna-are-involved-in-the-adherence-and-aggregation-of-the-extracellular-matrix
#8
I Randrianjatovo-Gbalou, P Rouquette, D Lefebvre, E Girbal-Neuhauser, C-E Marcato-Romain
AIMS: This study attempts to determine which exopolymeric substances are involved in the adherence and aggregation of a Bacillus licheniformis biofilm. METHODS AND RESULTS: The involvement of extracellular proteins and eDNA were particularly investigated using DNase and proteinase K treatment. The permeability of the biofilms increased 5-fold after DNase I-treatment. Quantification of the matrix components showed that, whatever the enzyme tested, some eDNA and amyloid-like polymers were removed simultaneously...
February 18, 2017: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/28207855/novel-approaches-for-the-taxonomic-and-metabolic-characterization-of-lactobacilli-integration-of-16s-rrna-gene-sequencing-with-maldi-tof-ms-and-1h-nmr
#9
Claudio Foschi, Luca Laghi, Carola Parolin, Barbara Giordani, Monica Compri, Roberto Cevenini, Antonella Marangoni, Beatrice Vitali
Lactobacilli represent a wide range of bacterial species with several implications for the human host. They play a crucial role in maintaining the ecological equilibrium of different biological niches and are essential for fermented food production and probiotic formulation. Despite the consensus about the 'health-promoting' significance of Lactobacillus genus, its genotypic and phenotypic characterization still poses several difficulties. The aim of this study was to assess the integration of different approaches, genotypic (16S rRNA gene sequencing), proteomic (MALDI-TOF MS) and metabolomic (1H-NMR), for the taxonomic and metabolic characterization of Lactobacillus species...
2017: PloS One
https://www.readbyqxmd.com/read/28198622/a-cell-penetrant-manganese-superoxide-dismutase-mnsod-mimic-is-able-to-complement-mnsod-and-exerts-an-antiinflammatory-effect-on-cellular-and-animal-models-of-inflammatory-bowel-diseases
#10
Emilie Mathieu, Anne-Sophie Bernard, Nicolas Delsuc, Elodie Quévrain, Géraldine Gazzah, Barry Lai, Florian Chain, Philippe Langella, Maria Bachelet, Joelle Masliah, Philippe Seksik, Clotilde Policar
Inorganic complexes are increasingly used for biological and medicinal applications, and the question of the cell penetration and distribution of metallodrugs is key to understanding their biological activity. Oxidative stress is known to be involved in inflammation and in inflammatory bowel diseases for which antioxidative defenses are weakened. We report here the study of the manganese complex Mn1 mimicking superoxide dismutase (SOD), a protein involved in cell protection against oxidative stress, using an approach in inorganic cellular chemistry combining the investigation of Mn1 intracellular speciation using mass spectrometry and of its quantification and distribution using electron paramagnetic resonance and spatially resolved X-ray fluorescence with evaluation of its biological activity...
February 15, 2017: Inorganic Chemistry
https://www.readbyqxmd.com/read/28160380/synthesis-of-bacteriophage-lytic-proteins-against-streptococcus-pneumoniae-in-the-chloroplast-of-chlamydomonas-reinhardtii
#11
Laura Stoffels, Henry N Taunt, Bambos Charalambous, Saul Purton
There is a pressing need to develop novel antibacterial agents given the widespread antibiotic resistance among pathogenic bacteria and the low specificity of the drugs available. Endolysins are antibacterial proteins that are produced by bacteriophage-infected cells to digest the bacterial cell wall for phage progeny release at the end of the lytic cycle. These highly efficient enzymes show a considerable degree of specificity for the target bacterium of the phage. Furthermore, the emergence of resistance against endolysins appears to be rare as the enzymes have evolved to target molecules in the cell wall that are essential for bacterial viability...
February 3, 2017: Plant Biotechnology Journal
https://www.readbyqxmd.com/read/28139746/a-sensitive-single-enzyme-assay-system-using-the-non-ribosomal-peptide-synthetase-bpsa-for-measurement-of-l-glutamine-in-biological-samples
#12
Alistair S Brown, Katherine J Robins, David F Ackerley
The ability to rapidly, economically and accurately measure L-glutamine concentrations in biological samples is important for many areas of research, medicine or industry, however there is room for improvement on existing methods. We describe here how the enzyme BpsA, a single-module non-ribosomal peptide synthetase able to convert L-glutamine into the blue pigment indigoidine, can be used to accurately measure L-glutamine in biological samples. Although indigoidine has low solubility in aqueous solutions, meaning direct measurements of indigoidine synthesis do not reliably yield linear standard curves, we demonstrate that resolubilisation of the reaction end-products in DMSO overcomes this issue and that spontaneous reduction to colourless leuco-indigoidine occurs too slowly to interfere with assay accuracy...
January 31, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28137573/rapid-proteomics-to-prospect-and-validate-novel-bacterial-metabolism-induced-by-environmental-burden
#13
C L Yu, S Brooks, Y Li, M Subramanian, R Summers, M Pope
Understanding the pathophysiology of genes and enzymes involved in caffeine metabolism can have extracurricular benefits, such as providing distinct methylxanthines as intermediates for pharmaceutical synthesis, and also improve environmental waste remediation. The strains Pseudomonas putida CBB5 and CES may provide insights into these applications because they may both be induced to degrade caffeine, yet the latter thrives in concentrations >8.0gL(-1); threefold higher than any other bacteria. We took a novel approach toward identifying the enzymatic pathways in both Pseudomonas sp...
2017: Methods in Enzymology
https://www.readbyqxmd.com/read/28109187/antimicrobial-and-antioxidant-activities-of-saccharomyces-cerevisiae-ifst062013-a-potential-probiotic
#14
Md Fakruddin, Md Nur Hossain, Monzur Morshed Ahmed
BACKGROUND: Probiotic yeast has become a field of interest to scientists in recent years. METHODS: Conventional cultural method was employed to isolate and identify yeast and standard methods were used to determine different probiotic attributes, antimicrobial and antioxidant properties. RESULTS: This study reports potential probiotic properties of a strain of S. cerevisiae IFST 062013 isolated from fruit. The isolate is tolerant to a wide range of temperature and pH, high concentration of bile salt and NaCl, gastric juice, intestinal environment, α-amylase, trypsin and lysozyme...
January 21, 2017: BMC Complementary and Alternative Medicine
https://www.readbyqxmd.com/read/28109129/vsl-3-probiotic-differently-influence-iec-6-intestinal-epithelial-cell-status-and-function
#15
REVIEW
Benedetta Cinque, Cristina La Torre, Francesca Lombardi, Paola Palumbo, Zoran Evtoski, Silvano Jr Santini, Stefano Falone, Annamaria Cimini, Fernanda Amicarelli, Maria Grazia Cifone
The data here reported introduce the wound-healing assay as a tool for testing probiotics aimed at protecting gastrointestinal mucosal surfaces and to verify the consistency of their manufacturing. At the scope, we compared the in vitro effects of two multi-strain high concentration formulations both commercialized under the same brand VSL#3 but sourced from different production sites (USA and Italy) on a non-transformed small-intestinal epithelial cell line, IEC-6. The effects on cellular morphology, viability, migration, and H2 O2 -induced damage, were assessed before and after the treatment with both VSL#3 formulations...
January 21, 2017: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/28094581/early-immunopathological-events-in-acute-model-of-mycobacterial-hypersensitivity-pneumonitis-in-mice
#16
Elisabet Johansson, Gregory P Boivin, Jagjit S Yadav
Prolonged exposure to antigens of non-tuberculous mycobacteria species colonizing industrial metalworking fluid (MWF), particularly Mycobacterium immunogenum (MI), has been implicated in chronic forms of hypersensitivity pneumonitis (HP) in machinists based on epidemiology studies and long-term exposure of mouse models. However, a role of short-term acute exposure to these antigens has not been described in the context of acute forms of HP. This study investigated short-term acute exposure of mice to MI cell lysate (or live cell suspension) via oropharyngeal aspiration...
December 2017: Journal of Immunotoxicology
https://www.readbyqxmd.com/read/28092851/plasmid-dna-affinity-partitioning-using-polyethylene-glycol-sodium-sulfate-aqueous-two-phase-systems
#17
Behzad Nazer, Mohammad Reza Dehghani, Bahram Goliaei
Extraction of plasmid DNA (pDNA) from an alkaline bacterial cell lysate has been investigated using partitioning and affinity partitioning in aqueous two-phase systems (ATPSs) containing 12% (w/w) polyethylene glycol and 12% (w/w) sodium sulfate. In this work, a modified 20bp pyrimidine oligonucleotide acting as a triplex-forming oligonucleotide (TFO) was employed as an affinity ligand to improve the partition behavior. The pUC118 plasmid was chosen to be extracted because of its specific oligonucleotide recognition sequence...
January 3, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/28081265/the-plasmin-sensitive-protein-pls-in-methicillin-resistant-staphylococcus-aureus-mrsa-is-a-glycoprotein
#18
Isabelle Bleiziffer, Julian Eikmeier, Gottfried Pohlentz, Kathryn McAulay, Guoqing Xia, Muzaffar Hussain, Andreas Peschel, Simon Foster, Georg Peters, Christine Heilmann
Most bacterial glycoproteins identified to date are virulence factors of pathogenic bacteria, i.e. adhesins and invasins. However, the impact of protein glycosylation on the major human pathogen Staphylococcus aureus remains incompletely understood. To study protein glycosylation in staphylococci, we analyzed lysostaphin lysates of methicillin-resistant Staphylococcus aureus (MRSA) strains by SDS-PAGE and subsequent periodic acid-Schiff's staining. We detected four (>300, ∼250, ∼165, and ∼120 kDa) and two (>300 and ∼175 kDa) glycosylated surface proteins with strain COL and strain 1061, respectively...
January 2017: PLoS Pathogens
https://www.readbyqxmd.com/read/28079559/strategies-to-alter-the-natural-history-of-childhood-asthma
#19
K A Lee-Sarwar, L B Bacharier, A A Litonjua
PURPOSE OF REVIEW: Asthma exhibits significant heterogeneity in occurrence and severity over the lifespan. Our goal is to discuss recent evidence regarding determinants of the natural history of asthma during childhood, and review the rationale behind and status of major efforts to alter its course. RECENT FINDINGS: Variations in microbial exposures are associated with risk of allergic disease, and the use of bacterial lysates may be a promising preventive strategy...
January 11, 2017: Current Opinion in Allergy and Clinical Immunology
https://www.readbyqxmd.com/read/28077440/monophosphoryl-lipid-a-enhances-efficacy-of-a-francisella-tularensis-lvs-catanionic-nanoparticle-subunit-vaccine-against-f-tularensis-schu-s4-challenge-by-augmenting-both-humoral-and-cellular-immunity
#20
Katharina Richard, Barbara J Mann, Aiping Qin, Eileen M Barry, Robert K Ernst, Stefanie N Vogel
Francisella tularensis, a bacterial biothreat agent, has no approved vaccine in the United States. Previously, we showed that incorporating lysates from partially attenuated F. tularensis LVS or fully virulent F. tularensis Schu S4 strains into catanionic surfactant vesicle (V) nanoparticles (LVS-V and Schu S4-V, respectively) protected fully against F. tularensis LVS intraperitoneal (i.p.) challenge in mice. However, we achieved only partial protection against F. tularensis Schu S4 intranasal (i.n.) challenge, even when employing heterologous prime-boost immunization strategies...
March 2017: Clinical and Vaccine Immunology: CVI
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