Tailoi Chan-Ling, Ping Hu, Sergio Li Calzi, Jeff Warner, Nasir Uddin, Mariana DuPont, Martha Neuringer, Paul Kievit, Lauren Renner, Jonathan Stoddard, Renee Ryals, Michael E Boulton, Trevor McGill, Maria B Grant
This study investigated retinal changes in a Western diet (WD)-induced non-human primate (NHP) model of Type 2 diabetes (T2D). Rhesus NHP aged 15-17 years old were fed a high fat diet (n=7) for more than 5 years with the aim of reflecting the traditional WD. Age-matched controls (n=6) were fed a standard laboratory primate diet. Retinal fundus, OCT, autofluorescence imaging and fluorescein angiography were undertaken prior to euthanasia. To assess diabetic retinopathy, eyes were examined using trypsin digests, lipofuscin autofluorescence and multi-marker immunofluorescence on cross sections and wholemounts...
March 23, 2023: American Journal of Pathology