keyword
https://read.qxmd.com/read/38536872/membrane-contact-sites-regulate-vacuolar-fission-via-sphingolipid-metabolism
#1
JOURNAL ARTICLE
Kazuki Hanaoka, Kensuke Nishikawa, Atsuko Ikeda, Philipp Schlarmann, Saku Sasaki, Sotaro Fujii, Sayumi Yamashita, Aya Nakaji, Kouichi Funato
Membrane contact sites (MCSs) are junctures that perform important roles including coordinating lipid metabolism. Previous studies have indicated that vacuolar fission/fusion processes are coupled with modifications in the membrane lipid composition. However, it has been still unclear whether MCS-mediated lipid metabolism controls the vacuolar morphology. Here, we report that deletion of tricalbins (Tcb1, Tcb2, and Tcb3), tethering proteins at endoplasmic reticulum (ER)-plasma membrane (PM) and ER-Golgi contact sites, alters fusion/fission dynamics and causes vacuolar fragmentation in the yeast Saccharomyces cerevisiae ...
March 27, 2024: ELife
https://read.qxmd.com/read/38536815/osbp-mediated-pi-4-p-cholesterol-exchange-at-endoplasmic-reticulum-secretory-granule-contact-sites-controls-insulin-secretion
#2
JOURNAL ARTICLE
Styliani Panagiotou, Kia Wee Tan, Phuoc My Nguyen, Andreas Müller, Affiong Ika Oqua, Alejandra Tomas, Anna Wendt, Lena Eliasson, Anders Tengholm, Michele Solimena, Olof Idevall-Hagren
Insulin is packaged into secretory granules that depart the Golgi and undergo a maturation process that involves changes in the protein and lipid composition of the granules. Here, we show that insulin secretory granules form physical contacts with the endoplasmic reticulum and that the lipid exchange protein oxysterol-binding protein (OSBP) is recruited to these sites in a Ca2+ -dependent manner. OSBP binding to insulin granules is positively regulated by phosphatidylinositol-4 (PI4)-kinases and negatively regulated by the PI4 phosphate (PI(4)P) phosphatase Sac2...
March 26, 2024: Cell Reports
https://read.qxmd.com/read/38536750/rchy1-and-optn-are-required-for-melanophagy-selective-autophagy-of-melanosomes
#3
JOURNAL ARTICLE
Ki Won Lee, Ki-Jun Ryu, Minju Kim, Seyeon Lim, Jisu Kim, Jeong Yoon Kim, Cheol Hwangbo, Jiyun Yoo, Yong-Yeon Cho, Kwang Dong Kim
Melanosomes are specific organelles dedicated to melanin synthesis and accumulation in melanocytes. Autophagy is suggestively involved in melanosome degradation, although the potential underlying molecular mechanisms remain elusive. In selective autophagy, autophagy receptors and E3-ligases are the key factors conferring cargo selectivity. In B16F10 cells, β-mangostin efficiently induced melanosome degradation without affecting other organelles such as mitochondria, peroxisomes, and the endoplasmic reticulum...
April 2, 2024: Proceedings of the National Academy of Sciences of the United States of America
https://read.qxmd.com/read/38536457/sbys1-and-sbwrky72-regulate-cd-tolerance-and-accumulation-in-sweet-sorghum
#4
JOURNAL ARTICLE
Weitao Jia, Zijing Guo, Sulian Lv, Kangqi Lin, Yinxin Li
SbYS1 and its upstream transcription factor SbWRKY72 were involved in Cd tolerance and accumulation and are valuable for developing sweet sorghum germplasm with high-Cd tolerance or accumulation ability through genetic manipulation. Cadmium (Cd) is highly toxic and can severely affect human health. Sweet sorghum, as an energy crop, shows great potential in extracting cadmium from Cd-contaminated soils. However, its molecular mechanisms of Cd-tolerance and -accumulation remain largely unknown. Here, we isolated a YSL family gene SbYS1 from the sweet sorghum genotype with high Cd accumulation ability and the expression of SbYS1 in roots was induced by cadmium...
March 27, 2024: Planta
https://read.qxmd.com/read/38536441/bridge-like-lipid-transfer-protein-family-member-2-suppresses-ciliogenesis
#5
JOURNAL ARTICLE
Jan Parolek, Christopher G Burd
Bridge-like lipid transfer protein family member 2 (BLTP2) is an evolutionary conserved protein with unknown function(s). The absence of BLTP2 in Drosophila melanogaster results in impaired cellular secretion and larval death, while in mice ( Mus musculus ), it causes preweaning lethality. Structural predictions propose that BLTP2 belongs to the repeating β-groove domain-containing (also called the VPS13) protein family, forming a long tube with a hydrophobic core, suggesting that it operates as a lipid transfer protein (LTP)...
March 27, 2024: Molecular Biology of the Cell
https://read.qxmd.com/read/38536006/downregulating-mirna-199a-5p-exacerbates-fluorouracil-induced-cardiotoxicity-by-activating-the-atf6-signaling-pathway
#6
JOURNAL ARTICLE
Wei Wang, Liang Dong, Hengxu Lv, Yonghui An, Changwang Zhang, Zheng Zheng, Ying Guo, Li He, Libin Wang, Jinmei Wang, Xinlei Shi, Na Li, Mingqi Zheng
BACKGROUND: Fluorouracil (5-FU) might produce serious cardiac toxic reactions. miRNA-199a-5p is a miRNA primarily expressed in myocardial cells and has a protective effect on vascular endothelium. Under hypoxia stress, the expression level of miRNA-199a-5p was significantly downregulated and is closely related to cardiovascular events such as coronary heart disease, heart failure, and hypertension. We explored whether 5-FU activates the endoplasmic reticulum stress ATF6 pathway by regulating the expression of miRNA-199a-5p in cardiac toxicity...
March 25, 2024: Aging
https://read.qxmd.com/read/38534785/cellular-organelle-related-transcriptomic-profile-abnormalities-in-neuronopathic-types-of-mucopolysaccharidosis-a-comparison-with-other-neurodegenerative-diseases
#7
JOURNAL ARTICLE
Karolina Wiśniewska, Lidia Gaffke, Magdalena Żabińska, Grzegorz Węgrzyn, Karolina Pierzynowska
Mucopolysaccharidoses (MPS) are a group of diseases caused by mutations in genes encoding lysosomal enzymes that catalyze reactions of glycosaminoglycan (GAG) degradation. As a result, GAGs accumulate in lysosomes, impairing the proper functioning of entire cells and tissues. There are 14 types/subtypes of MPS, which are differentiated by the kind(s) of accumulated GAG(s) and the type of a non-functional lysosomal enzyme. Some of these types (severe forms of MPS types I and II, MPS III, and MPS VII) are characterized by extensive central nervous system disorders...
March 21, 2024: Current Issues in Molecular Biology
https://read.qxmd.com/read/38534758/autophagy-dependent-secretion-crosstalk-between-autophagy-and-exosome-biogenesis
#8
REVIEW
Ekaterina Zubkova, Alexander Kalinin, Anastasya Bolotskaya, Irina Beloglazova, Mikhail Menshikov
The cellular secretome is pivotal in mediating intercellular communication and coordinating responses to stressors. Exosomes, initially recognized for their role in waste disposal, have now emerged as key intercellular messengers with significant therapeutic and diagnostic potential. Similarly, autophagy has transcended its traditional role as a waste removal mechanism, emerging as a regulator of intracellular communication pathways and a contributor to a unique autophagy-dependent secretome. Secretory authophagy, initiated by various stress stimuli, prompts the selective release of proteins implicated in inflammation, including leaderless proteins that bypass the conventional endoplasmic reticulum-Golgi secretory pathway...
March 8, 2024: Current Issues in Molecular Biology
https://read.qxmd.com/read/38534743/effects-of-letrozole-treatment-and-vitamin-c-supplementation-on-morphology-endoplasmic-reticulum-stress-programmed-cell-death-and-oxidative-stress-in-the-small-intestine-of-adult-male-rats
#9
JOURNAL ARTICLE
Anna Pilutin, Sylwia Rzeszotek, Aleksandra Wilk, Klaudia Klimaszewska, Julia Łukasiewicz, Rufaro Lynnette Mafuta, Thanushan Nagendran, Rupia Ndambara, Barbara Wiszniewska
Estrogens are hormones that play an important role in the digestive tract, including in men. Letrozole is an inhibitor of cytochrome P450 aromatase, an enzyme converting androgens to estrogens. The use of letrozole may cause oxidative stress and endoplasmic reticulum stress in the cells. Factors modulating cellular stress may include vitamin C. The purpose of this study was to examine whether letrozole and/or vitamin C supplementation can affect the morphology of the small intestine, the parameters of endoplasmic reticulum stress, programmed cell death markers, and oxidative damage...
March 1, 2024: Current Issues in Molecular Biology
https://read.qxmd.com/read/38534732/induction-of-endoplasmic-reticulum-stress-by-prodigiosin-in-yeast-saccharomyces-cerevisiae
#10
JOURNAL ARTICLE
Sy Le Thanh Nguyen, Thi Hien Trang Nguyen, Thi Tuyen Do, Thi Thao Nguyen, Thanh Hoang Le, Thi Anh Tuyet Nguyen, Yukio Kimata
Prodigiosin, a red pigment produced by numerous bacterial species, exerts various antibiotic effects on prokaryotic and eukaryotic organisms. For instance, human carcinoma cell lines appear to suffer from endoplasmic reticulum (ER) stress in the presence of prodigiosin. Here, we demonstrated that prodigiosin also triggers the unfolded-protein response (UPR), which is a cytoprotective response against ER stress, in yeast Saccharomyces cerevisiae . An S. cerevisiae mutant carrying a UPR-deficient mutation was hypersensitive to prodigiosin...
February 26, 2024: Current Issues in Molecular Biology
https://read.qxmd.com/read/38534723/the-role-of-aminopeptidase-erap1-in-human-pathology-a-review
#11
REVIEW
Laura Țiburcă, Dana Carmen Zaha, Maria Claudia Jurca, Emilia Severin, Aurora Jurca, Alexandru Daniel Jurca
Aminopeptidases are a group of enzymatic proteins crucial for protein digestion, catalyzing the cleavage of amino acids at the N-terminus of peptides. Among them are ERAP1 (coding for endoplasmic reticulum aminopeptidase 1), ERAP2 (coding for endoplasmic reticulum aminopeptidase 2), and LNPEP (coding for leucyl and cystinyl aminopeptidase). These genes encoding these enzymes are contiguous and located on the same chromosome (5q21); they share structural homology and functions and are associated with immune-mediated diseases...
February 20, 2024: Current Issues in Molecular Biology
https://read.qxmd.com/read/38534475/enhancing-antibody-specific-productivity-unraveling-the-impact-of-xbp1s-overexpression-and-glutamine-availability-in-sp2-0-cells
#12
JOURNAL ARTICLE
Priscilla González-Pereira, Ryan Trinh, Alex Vasuthasawat, Angelo Bartsch-Jiménez, Constanza Nuñez-Soto, Claudia Altamirano
Augmentation of glycoprotein synthesis requirements induces endoplasmic reticulum (ER) stress, activating the unfolded protein response (UPR) and triggering unconventional XBP1 splicing. As a result, XBP1s orchestrates the expression of essential genes to reduce stress and restore homeostasis. When this mechanism fails, chronic stress may lead to apoptosis, which is thought to be associated with exceeding a threshold in XBP1s levels. Glycoprotein assembly is also affected by glutamine (Gln) availability, limiting nucleotide sugars (NS), and preventing compliance with the increased demands...
February 21, 2024: Bioengineering
https://read.qxmd.com/read/38534366/steroidogenesis-upregulation-through-mitochondria-associated-endoplasmic-reticulum-membranes-and-mitochondrial-dynamics-in-rat-testes-the-role-of-d-aspartate
#13
JOURNAL ARTICLE
Debora Latino, Massimo Venditti, Sara Falvo, Giulia Grillo, Alessandra Santillo, Imed Messaoudi, Mariem Ben Rhouma, Sergio Minucci, Gabriella Chieffi Baccari, Maria Maddalena Di Fiore
Mitochondria-Associated Endoplasmic Reticulum Membranes (MAMs) mediate the communication between the Endoplasmic Reticulum (ER) and the mitochondria, playing a fundamental role in steroidogenesis. This study aimed to understand how D-aspartate (D-Asp), a well-known stimulator of testosterone biosynthesis and spermatogenesis, affects the mechanism of steroidogenesis in rat testes. Our results suggested that D-Asp exerts this function through MAMs, affecting lipid trafficking, calcium signaling, ER stress, and mitochondrial dynamics...
March 16, 2024: Cells
https://read.qxmd.com/read/38534320/phosphodiesterases-4b-and-4d-differentially-regulate-camp-signaling-in-calcium-handling-microdomains-of-mouse-hearts
#14
JOURNAL ARTICLE
Axel E Kraft, Nadja I Bork, Hariharan Subramanian, Nikoleta Pavlaki, Antonio V Failla, Bernd Zobiak, Marco Conti, Viacheslav O Nikolaev
The ubiquitous second messenger 3',5'-cyclic adenosine monophosphate (cAMP) regulates cardiac excitation-contraction coupling (ECC) by signaling in discrete subcellular microdomains. Phosphodiesterase subfamilies 4B and 4D are critically involved in the regulation of cAMP signaling in mammalian cardiomyocytes. Alterations of PDE4 activity in human hearts has been shown to result in arrhythmias and heart failure. Here, we sought to systematically investigate specific roles of PDE4B and PDE4D in the regulation of cAMP dynamics in three distinct subcellular microdomains, one of them located at the caveolin-rich plasma membrane which harbors the L-type calcium channels (LTCCs), as well as at two sarco/endoplasmic reticulum (SR) microdomains centered around SR Ca2+ -ATPase (SERCA2a) and cardiac ryanodine receptor type 2 (RyR2)...
March 8, 2024: Cells
https://read.qxmd.com/read/38533111/a-transcriptomic-dataset-for-investigating-the-arabidopsis-unfolded-protein-response-under-chronic-proteotoxic-endoplasmic-reticulum-stress
#15
JOURNAL ARTICLE
Amélie Ducloy, Marianne Azzopardi, Caroline Ivsic, Gwendal Cueff, Delphine Sourdeval, Delphine Charif, Jean-Luc Cacas
The Unfolded Protein Response (UPR) is a retrograde, ER-to-nucleus, signalling pathway which is conserved across kingdoms. In plants, it contributes to development, reproduction, immunity and tolerance to abiotic stress. This RNA sequencing (RNA-seq) dataset was produced from 14-day-old Arabidopsis thaliana seedlings challenged by tunicamycin (Tm), an antibiotic inhibiting Asn-linked glycosylation in the endoplasmic reticulum (ER), causing an ER stress and eventually activating the UPR. Wild-type (WT) and a double mutant deficient for two main actors of the UPR ( INOSITOL-REQUIRING ENZYME 1A and INOSITOL-REQUIRING ENZYME 1B ) were used as genetic backgrounds in our experimental setup, allowing to distinguish among differentially-expressed genes (DEGs) which ones are dependent on or independent on IRE1s...
April 2024: Data in Brief
https://read.qxmd.com/read/38532011/active-forgetting-and-neuropsychiatric-diseases
#16
REVIEW
Jacob A Berry, Dana C Guhle, Ronald L Davis
Recent and pioneering animal research has revealed the brain utilizes a variety of molecular, cellular, and network-level mechanisms used to forget memories in a process referred to as "active forgetting". Active forgetting increases behavioral flexibility and removes irrelevant information. Individuals with impaired active forgetting mechanisms can experience intrusive memories, distressing thoughts, and unwanted impulses that occur in neuropsychiatric diseases. The current evidence indicates that active forgetting mechanisms degrade, or mask, molecular and cellular memory traces created in synaptic connections of "engram cells" that are specific for a given memory...
March 26, 2024: Molecular Psychiatry
https://read.qxmd.com/read/38531909/preso-enhances-mglur1-mediated-excitotoxicity-by-modulating-the-phosphorylation-of-mglur1-homer1-complex-and-facilitating-an-er-stress-after-traumatic-brain-injury
#17
JOURNAL ARTICLE
Zhuoyuan Zhang, Xiangyu Gao, Zhicheng Tian, Erwan Yang, Yutao Huang, Dan Liu, Shuhui Dai, Haofuzi Zhang, Mingdong Bao, Xiaofan Jiang, Xin Li, Peng Luo
Glutamate receptor (GluR)-mediated excitotoxicity is an important mechanism causing delayed neuronal injury after traumatic brain injury (TBI). Preso, as a core scaffolding protein of postsynaptic density (PSD), is considered an important regulator during excitotoxicity and TBI and combines with glutamate receptors to form functional units for excitatory glutamatergic neurotransmission, and elucidating the mechanisms of these functional units will provide new targets for the treatment of TBI. As a multidomain scaffolding protein, Preso directly interacts with metabotropic GluR (mGluR) and another scaffold protein, Homer...
March 26, 2024: Cell Death Discovery
https://read.qxmd.com/read/38531904/social-defeat-stress-induces-liver-injury-by-modulating-endoplasmic-reticulum-stress-in-c57bl-6j-mice
#18
JOURNAL ARTICLE
XiaoLei Gao, Tong Zhao, Ran Hao, ZhaoHui Zhang, Guang-Biao Huang
Social defeat stress is associated with endoplasmic reticulum (ER) stress, inflammation and apoptosis. ER stress is thought to contribute to many lifestyle diseases such as liver injury, cardiovascular dysfunction and depression. We investigated the expression of the ER stress markers RNA-dependent protein kinase-like ER kinase (PERK), eukaryotic translation initiation factor 2α (eIF2α) and C/EBP homologous protein (CHOP), as well as inflammatory and apoptotic factors, to assess how social defeat stress induces liver injury...
March 26, 2024: Scientific Reports
https://read.qxmd.com/read/38531832/molecular-mechanisms-involved-in-alcohol-craving-irf3-and-endoplasmic-reticulum-stress-a-multi-omics-study
#19
JOURNAL ARTICLE
Ming-Fen Ho, Cheng Zhang, Irene Moon, Mustafa Tuncturk, Brandon J Coombes, Joanna Biernacka, Michelle Skime, Tyler S Oesterle, Victor M Karpyak, Hu Li, Richard Weinshilboum
Alcohol use disorder (AUD) is the most prevalent substance use disorder worldwide. Acamprosate and naltrexone are anti-craving drugs used in AUD pharmacotherapy. However, molecular mechanisms underlying their anti-craving effect remain unclear. This study utilized a patient-derived induced pluripotent stem cell (iPSC)-based model system and anti-craving drugs that are used to treat AUD as "molecular probes" to identify possible mechanisms associated with alcohol craving. We examined the pathophysiology of craving and anti-craving drugs by performing functional genomics studies using iPSC-derived astrocytes and next-generation sequencing...
March 26, 2024: Translational Psychiatry
https://read.qxmd.com/read/38531818/inhibition-of-the-endoplasmic-reticulum-stress-associated-ire-1-pathway-alleviates-preterm-birth
#20
JOURNAL ARTICLE
Liyin Qiu, Hui Liu, Shali Chen, Yiting Wu, Jianying Yan
BACKGROUND: Premature birth (PTB) remains a major global health concern due to its association with neonatal morbidity and mortality. The unfolded protein response (UPR) within the endoplasmic reticulum (ER) is tightly regulated by Inositol-requiring enzyme type 1 (IRE-1), a pivotal cellular modulator. This study seeks to elucidate the role of the ER stress (ERS)-related IRE-1 pathway in PTB. METHODS: Human placental trophoblast cells HTR8/Svneo were exposed to the ER-stress inducer tunicamycin (TM)...
March 2024: American Journal of Reproductive Immunology: AJRI
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