keyword
MENU ▼
Read by QxMD icon Read
search

Tumor-derived exosomes

keyword
https://www.readbyqxmd.com/read/29030127/a-method-for-the-isolation-and-enrichment-of-purified-bovine-milk-exosomes
#1
Kanchan Vaswani, Yong Qin Koh, Fatema B Almughlliq, Hassendrini N Peiris, Murray D Mitchell
Exosomes are nanovesicles that play important roles in intercellular communication as they carry information to target cells. Isolation of high purity exosomes will aid in studying the exosomal cargo and quantity as well as how cell-specific messages are carried. We describe a new method incorporating size exclusion chromatography (SEC) to enrich milk-derived exosomes from extracellular vesicles (EVs). This involved the initial isolation of EVs from bovine milk via milk processing and ultracentrifugation; followed by a new method to enrich exosomes using SEC...
October 10, 2017: Reproductive Biology
https://www.readbyqxmd.com/read/29029605/monitoring-multiple-myeloma-by-idiotype-specific-peptide-binders-of-tumor-derived-exosomes
#2
Enrico Iaccino, Selena Mimmi, Vincenzo Dattilo, Fabiola Marino, Patrizio Candeloro, Antonio Di Loria, Danilo Marimpietri, Antonio Pisano, Francesco Albano, Eleonora Vecchio, Simona Ceglia, Gaetanina Golino, Antonio Lupia, Giuseppe Fiume, Ileana Quinto, Giuseppe Scala
Tumor-derived exosomes (TDEs) play a pivotal role in tumor establishment and progression, and are emerging biomarkers for tumor diagnosis in personalized medicine. To date, there is a lack of efficient technology platforms for exosome isolation and characterization. Multiple myeloma (MM) is an incurable B-cell malignancy due to the rapid development of drug-resistance. MM-released exosomes express the immunoglobulin B-cell receptor (Ig-BCR) of the tumor B-cells, which can be targeted by Idiotype-binding peptides (Id-peptides)...
October 13, 2017: Molecular Cancer
https://www.readbyqxmd.com/read/29023935/exosome-mir-335-as-a-novel-therapeutic-strategy-in-hepatocellular-carcinoma
#3
Fang Wang, Tian Hongwei, Ling Li, Klaus Piontek, Masazumi Sakaguchi, Florin M Selaru
Hepatocellular Cancer (HCC) is a common and deadly cancer. Most cases of HCC arise in a cirrhotic/fibrotic liver, raising the possibility that the environment plays a paramount role in cancer genesis. Previous studies from our group and others have shown that, in desmoplastic cancers, there is a rich intercellular communication between activated, cancer-associated fibroblasts (CAF) and cancer cells. Moreover, Extracellular Vesicles (EVs), or exosomes, were identified asan important arm of this intercellular communication platform...
October 10, 2017: Hepatology: Official Journal of the American Association for the Study of Liver Diseases
https://www.readbyqxmd.com/read/29019651/combining-machine-learning-and-nanofluidic-technology-to-diagnose-pancreatic-cancer-using-exosomes
#4
Jina Ko, Neha Bhagwat, Stephanie S Yee, Natalia Ortiz, Amine Sahmoud, Taylor Black, Nicole M Aiello, Lydie McKenzie, Mark O'Hara, Colleen Redlinger, Janae Romeo, Erica L Carpenter, Ben Z Stanger, David Issadore
Circulating exosomes contain a wealth of proteomic and genetic information, presenting an enormous opportunity in cancer diagnostics. While microfluidic approaches have been used to successfully isolate cells from complex samples, scaling these approaches for exosome isolation has been limited by the low throughput and susceptibility to clogging of nanofluidics. Moreover, the analysis of exosomal biomarkers is confounded by substantial heterogeneity between patients and within a tumor itself. To address these challenges, we developed a multi-channel nanofluidic system to analyze crude clinical samples...
October 11, 2017: ACS Nano
https://www.readbyqxmd.com/read/29016925/divergent-evolution-of-temozolomide-resistance-in-glioblastoma-stem-cells-is-reflected-in-extracellular-vesicles-and-coupled-with-radiosensitization
#5
Delphine Garnier, Brian Meehan, Thomas Kislinger, Paul Daniel, Ankit Sinha, Bassam Abdulkarim, Ichiro Nakano, Janusz Rak
Background: Glioblastoma (GBM) is almost invariably fatal due to failure of standard therapy. The relapse of GBM following surgery, radiation and systemic temozolomide (TMZ) is attributed to the ability of glioma stem cells (GSCs) to survive, evolve and repopulate the tumor mass, events on which therapy exerts a poorly understood influence. Methods: Here we explore the molecular and cellular evolution of TMZ resistance as it emerges in vivo (xenograft models) in a series of human GSCs with either proneural (PN) or mesenchymal (MES) molecular characteristics...
July 28, 2017: Neuro-oncology
https://www.readbyqxmd.com/read/29016843/mesenchymal-stem-cells-as-natural-bio-factories-for-exosomes-carrying-mir-124a-in-the-treatment-of-gliomas
#6
Frederick M Lang, Anwar Hossain, Joy Gumin, Eric N Momin, Yuzaburo Shimizu, Dan Ledbetter, Tal Shahar, Shinji Yamashita, Brittany Parker-Kerrigan, Juan Fueyo, Raymond Sawaya, Frederick F Lang
Background: MicroRNAs (miRs) are promising new therapeutics for glioblastoma. However, which miRs are most effective against glioblastomas and how these miRs should be delivered are major unanswered problems. Methods: To identify potent anti-glioma miRs, we selected eight miRs based on a literature search and screened them against a panel of glioma stem cell lines (GSCs), representing all TCGA-defined glioblastoma subtypes. To address delivery, we tested the hypothesis that ex vivo-cultured bone marrow-derived mesenchymal stem cells (MSCs) can package miRs into exosomes and that these engineered exosomes can systemically deliver antiglioma miRs to glioblastomas...
August 14, 2017: Neuro-oncology
https://www.readbyqxmd.com/read/28991298/exosomal-delivery-of-berry-anthocyanidins-for-the-management-of-ovarian-cancer
#7
Farrukh Aqil, Jeyaprakash Jeyabalan, Ashish K Agrawal, Al-Hassan Kyakulaga, Radha Munagala, Lynn Parker, Ramesh C Gupta
Despite optimal diagnosis and early therapeutic interventions, the prognosis for ovarian cancer patients remains dismal because the efficacy of chemotherapy is limited by the development of resistance and off-site toxicity. Berry bioactives indicate preventive and therapeutic activities against various cancer types. Here, we examined the antiproliferative activity of berry anthocyanidins (Anthos) against drug-sensitive (A2780) and drug-resistant (A2780/CP70, OVCA432 and OVCA433) ovarian cancer cells. These drug-resistant ovarian cancer cell lines overexpress p-glycoproteins (PgP) and show >100-fold resistance to the chemotherapeutic drug cisplatin compared to A2780...
October 9, 2017: Food & Function
https://www.readbyqxmd.com/read/28983919/role-of-stem-cell-derived-exosomes-in-tumor-biology
#8
REVIEW
Aman Sharma
Exosomes are nano-scale messengers loaded with bio-molecular cargo of RNA, DNA, and Proteins. As a master regulator of cellular signalling, stem cell (both normal, and cancer stem cells) secreted exosome orchestrate various autocrine and paracrine functions which alter tumor micro-environment, growth and progression. Exosomes secreted by one of the two important stem cell phenotypes in cancers a) Mesenchymal stem cells, and b) Cancer stem cells not only promote cancerous growth but also impart therapy resistance in cancer cells...
October 6, 2017: International Journal of Cancer. Journal International du Cancer
https://www.readbyqxmd.com/read/28981528/racial-differences-in-the-expression-of-inhibitors-of-apoptosis-iap-proteins-in-extracellular-vesicles-ev-from-prostate-cancer-patients
#9
Salma Khan, Jennifer Simpson, James C Lynch, David Turay, Saied Mirshahidi, Amber Gonda, Tino W Sanchez, Carlos A Casiano, Nathan R Wall
African-American men with prostate cancer typically develop more aggressive tumors than men from other racial/ethnic groups, resulting in a disproportionately high mortality from this malignancy. This study evaluated differences in the expression of inhibitors of apoptosis proteins (IAPs), a known family of oncoproteins, in blood-derived exosomal vesicles (EV) between African-American and European-American men with prostate cancer. The ExoQuick™ method was used to isolate EV from both plasma and sera of African-American (n = 41) and European-American (n = 31) men with prostate cancer, as well as from controls with no cancer diagnosis (n = 10)...
2017: PloS One
https://www.readbyqxmd.com/read/28981169/mir-200c-3p-spreads-invasive-capacity-in-human-oral-squamous-cell-carcinoma-microenvironment
#10
Tomoyo Kawakubo-Yasukochi, Masahiko Morioka, Mai Hazekawa, Atsushi Yasukochi, Takuya Nishinakagawa, Kazuhiko Ono, Shintaro Kawano, Seiji Nakamura, Manabu Nakashima
Oral squamous cell carcinoma (OSCC) constitutes over 90% of all cancers in the oral cavity. The prognosis for patients with invasive OSCC is poor; therefore, it is important to understand the molecular mechanisms of invasion and subsequent metastasis not only to prevent cancer progression but also to detect new therapeutic targets against OSCC. Recently, extracellular vesicles-particularly exosomes-have been recognized as intercellular communicators in the tumor microenvironment. As exosomic cargo, deregulated microRNAs (miRNAs) can shape the surrounding microenvironment in a cancer-dependent manner...
October 5, 2017: Molecular Carcinogenesis
https://www.readbyqxmd.com/read/28977747/enhanced-class-i-tumor-antigen-presentation-via-cytosolic-delivery-of-exosomal-cargos-by-tumor-cell-derived-exosomes-displaying-a-ph-sensitive-fusogenic-peptide
#11
Masaki Morishita, Yuki Takahashi, Makiya Nishikawa, Reiichi Ariizumi, Yoshinobu Takakura
Tumor cell-derived exosomes contain endogenous tumor antigens and can be used as a potential cancer vaccine without requiring identification of the tumor-specific antigen. To elicit an effective antitumor effect, efficient tumor antigen presentation by MHC class I molecules on dendritic cells (DC) is desirable. Because DC endocytose exosomes, an endosomal escape mechanism is required for efficient MHC class I presentation of exosomal tumor antigens. In the present study, efficient cytosolic delivery of exosomal tumor antigens was performed using genetically engineered tumor cell-derived exosomes and pH-sensitive fusogenic GALA peptide...
October 4, 2017: Molecular Pharmaceutics
https://www.readbyqxmd.com/read/28969005/pancreatic-cancer-derived-exosomes-promote-tumor-metastasis-and-liver-pre-metastatic-niche-formation
#12
Zeqian Yu, Susu Zhao, Long Ren, Lishan Wang, Zhangjun Chen, Robert M Hoffman, Jiahua Zhou
Exosomes play important roles in cell-cell communication, and are likely mediators of the metastatic cascade in cancer. This study examined the role of exosomes in pancreatic cancer cell adhesion, migration, and invasion. We isolated and purified exosomes from two isogenic pancreatic cancer cell lines with different metastatic potentials. Uptake of exosomes from highly metastatic Panc02-H7 cells decreased adhesion and increased migration and invasion capacity in weakly metastatic Panc02 cells in vitro. Exosomes from highly metastatic pancreatic cancer cells induced liver pre-metastatic niche formation in naïve mice and promoted primary tumor growth and liver metastasis in vivo...
September 8, 2017: Oncotarget
https://www.readbyqxmd.com/read/28963969/intercellular-resistance-to-braf-inhibition-can-be-mediated-by-extracellular-vesicle-associated-pdgfr%C3%AE
#13
Laura J Vella, Andreas Behren, Bradley Coleman, David W Greening, Andrew F Hill, Jonathan Cebon
Treatment of BRAF mutant melanoma with kinase inhibitors has been associated with rapid tumor regression; however, this clinical benefit is short-lived, and most patients relapse. A number of studies suggest that the extracellular environment promotes BRAF inhibitor resistance and tumor progression. Extracellular vesicles, such as exosomes, are functional mediators in the extracellular environment. They are small vesicles known to carry a concentrated group of functional cargo and serve as intercellular communicators not only locally but also systemically...
September 27, 2017: Neoplasia: An International Journal for Oncology Research
https://www.readbyqxmd.com/read/28950217/breast-cancer-extracellular-vesicles-induce-platelet-activation-and-aggregation-by-tissue-factor-independent-and-dependent-mechanisms
#14
Fausto G Gomes, Vanessa Sandim, Vitor H Almeida, Araci M R Rondon, Barbara B Succar, Eugenio D Hottz, Ana Carolina Leal, Brunno Renato F Verçoza, Juliany Cola F Rodrigues, Patrícia T Bozza, Russolina B Zingali, Robson Q Monteiro
INTRODUCTION: Cancer-associated thrombosis is one of the major causes of worse prognosis among tumor-bearing patients. Extracellular vesicles derived from cancer cells, which can be divided mainly into microvesicles and exosomes, can participate in several tumor progression phenomena. Tumor-derived microvesicles positive for tissue factor (TF) have been associated with thrombotic risk in certain cancer types. Cancer cell-derived exosomes, however, have not. In this study we evaluated the capacity of extracellular vesicles (EVs, containing both microvesicles and exosomes) derived from breast-cancer cell lines in promoting platelet activation, aggregation and plasma coagulation, in experiments that access both TF-dependent and -independent activities...
September 21, 2017: Thrombosis Research
https://www.readbyqxmd.com/read/28948499/cd133-positive-u87-glioblastoma-cells-derived-exosomal-micrornas-in-hypoxia-versus-normoxia-microenviroment
#15
Guobin Zhang, Yunsheng Zhang, Sen Cheng, Zhen Wu, Fusheng Liu, Junting Zhang
Hypoxia is a major regulator of glioma development and aggressiveness. However, how CD133 positive U87 glioblastoma cells adapt to hypoxia and communicate with their surrounding microenvironment during tumor development remain important questions. Communication with host cells and stroma via exosomes represents one pathway by which tumors can modify their surroundings to achieve a tumor-permissive environment. MicroRNAs are thought to be essential actors of tumorigenesis as they are able to control the expression of numerous genes...
September 25, 2017: Journal of Neuro-oncology
https://www.readbyqxmd.com/read/28947140/co-delivery-of-tumor-derived-exosomes-with-alpha-galactosylceramide-on-dendritic-cell-based-immunotherapy-for-glioblastoma
#16
Hongyu Liu, Ling Chen, Jialin Liu, Hengxing Meng, Rong Zhang, Lin Ma, Liangliang Wu, Songyan Yu, Fei Shi, Ying Li, Lijun Zhang, Lingxiong Wang, Shiyu Feng, Qi Zhang, Yaojun Peng, Qiyan Wu, Chunxi Liu, Xin Chang, Lin Yang, Xinguang Yu, Tianyi Liu
Dendritic cell (DC) vaccine-based immunotherapy for glioblastoma multiforme (GBM) has shown apparent benefit in animal experiments and early-phase clinical trials, but the survival benefit is variable. In this work, we analyzed the mechanism of the potent antitumor immune response induced in vivo by tumor-associated antigen (TAA)-specific DCs with an invariant natural killer T cell (iNKT) adjuvant in orthotopic glioblastoma-bearing rats vaccinated with tumor-derived exosomes and α-galactosylceramide (α-GalCer) -pulsed DCs...
September 23, 2017: Cancer Letters
https://www.readbyqxmd.com/read/28933725/integrins-and-exosomes-a-dangerous-liaison-in-cancer-progression
#17
REVIEW
Mayra Paolillo, Sergio Schinelli
Integrin activity and function is classically related to the bi-directional regulation of cell-extracellular matrix (ECM) contacts that regulate a number of cell pathways linked to cell adhesion, cell detachment from ECM, cell migration, and anoikis. Interestingly, emerging data continue to uncover new roles for integrins in cancer-relevant pathways, particularly concerning the regulation of immune cell activity in the tumor niche, like myeloid cell differentiation and function and, very recently, the regulation of metastatic processes by exosomes...
July 26, 2017: Cancers
https://www.readbyqxmd.com/read/28931213/a-review-on-the-efficacy-and-toxicity-of-different-doxorubicin-nanoparticles-for-targeted-therapy-in-metastatic-breast-cancer
#18
REVIEW
Ayman Shafei, Wesam El-Bakly, Ahmed Sobhy, Omar Wagdy, Ahmed Reda, Omar Aboelenin, Amr Marzouk, Khalil El Habak, Randa Mostafa, Mahmoud A Ali, Mahmoud Ellithy
In metastatic breast cancer (MBC), the conventional doxorubicin (DOX) has various problems due to lack of selectivity with subsequent therapeutic failure and adverse effects. DOX- induced cardiotoxicity is a major problem that necessitates the presence of new forms to decrease the risk of associated morbidity. Nanoparticles (NPs) are considered an important approach to selectively increase drug accumulation inside tumor cells and thus decreasing the associated side effects. Tumor cells develop resistance to chemotherapeutic agents through multiple mechanisms, one of which is over expression of efflux transporters...
September 16, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28929578/horizontal-transfer-of-mir-23a-from-hypoxic-tumor-cell-colonies-can-induce-angiogenesis
#19
T V Sruthi, Lincy Edatt, Grace R Raji, Haritha K, S Sharath Shankar, Vandana Shankar, Vishnu R, Aswini P, V B Sameer Kumar
Neo vessel formation by angiogenesis is an important event during many pathological conditions including cancer, where it is indispensable for tumor growth and survival. Although, various pro-angiogenic cytokines and soluble factors, secreted by tumor cells, have been reported to promote angiogenesis, recent studies have shown regulatory role of exosomes, secreted by tumor cells in the process of angiogenesis. These exosomes are capable of carrying nucleic acids, proteins etc. as their cargo. Under the light of these facts and considering the presence of miRNAs, the non-coding RNAs capable of regulating target gene expression, as one of the major cargos in the exosomes, we investigated, whether exosomes derived from normoxic and hypoxic tumor cell colonies exhibit difference in levels of miR-23∼27∼24 cluster members and if so, to check the significance of their horizontall transfer on the process of angiogenesis...
September 20, 2017: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/28920705/human-endothelial-progenitor-cell-derived-exosomes-increase-proliferation-and-angiogenesis-in-cardiac-fibroblasts-by-promoting-the-mesenchymal-endothelial-transition-and-reducing-high-mobility-group-box-1-protein-b1-expression
#20
Xiao Ke, Dahao Yang, Jiawen Liang, Xing Wang, Shaoyun Wu, Xiaoqing Wang, Chengheng Hu
Myocardial fibrosis is a characteristic feature of cardiomyopathies. However, no effective strategies to attenuate cardiac fibrosis are currently available. Late-stage endothelial progenitor cells (EPCs) are precursors of endothelial cells (ECs) that repair the heart through a paracrine mechanism. In the present study, we tested whether EPC-derived exosomes regulate the differentiation of fibroblasts into ECs. We isolated late-stage EPCs from human peripheral blood (PB) and used immunofluorescence and flow cytometry to confirm their identity...
September 18, 2017: DNA and Cell Biology
keyword
keyword
73941
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"