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https://www.readbyqxmd.com/read/29433005/cytotoxic-activity-and-structural-features-of-ru-ii-phosphine-amino-acid-complexes
#1
Edjane R Dos Santos, Angelica E Graminha, Mario S Schultz, Isabel Correia, Heloisa S Selistre-de-Araújo, Rodrigo S Corrêa, Javier Ellena, Elisângela de Paula S Lacerda, João Costa Pessoa, Alzir A Batista
Thirteen new ruthenium amino acid complexes were synthesized and characterized. They were obtained by the reaction of α-amino acids (AA) with [RuCl2(P-P)(N-N)], where P-P=1,4-bis(diphenylphosphino)butane (dppb) or 1,3-bis(diphenylphosphino)propane (dppp) and N-N=4,4'-dimethyl-2,2'-bipyridine (4'-Mebipy), 5,5'-dimethyl-2,2'-bipyridine (5'-Mebipy) or 4,4'-Methoxy-2-2'-bipyridine (4'-MeObipy). This afforded a family of complexes formulated as [Ru(AA-H)(P-P)(N-N)]PF6, where AA=glycine (Gly), L-alanine (Ala), L-valine (Val), L-tyrosine (Tyr), L-tryptophan (Trp), L-histidine (His) and L-methionine (Met)...
December 23, 2017: Journal of Inorganic Biochemistry
https://www.readbyqxmd.com/read/29402284/metabolic-characterization-of-menopause-cross-sectional-and-longitudinal-evidence
#2
Qin Wang, Diana L Santos Ferreira, Scott M Nelson, Naveed Sattar, Mika Ala-Korpela, Debbie A Lawlor
BACKGROUND: Women who experience menopause are at higher cardiometabolic risk and often display adverse changes in metabolic biomarkers compared with pre-menopausal women. It remains elusive whether the changes in cardiometabolic biomarkers during the menopausal transition are due to ovarian aging or chronological aging. Well-conducted longitudinal studies are required to determine this. The aim of this study was to explore the cross-sectional and longitudinal associations of reproductive status, defined according to the 2012 Stages of Reproductive Aging Workshop criteria, with 74 metabolic biomarkers, and establish whether any associations are independent of age-related changes...
February 6, 2018: BMC Medicine
https://www.readbyqxmd.com/read/29397160/effect-of-dietary-counseling-on-a-comprehensive-metabolic-profile-from-childhood-to-adulthood
#3
Miia Lehtovirta, Katja Pahkala, Harri Niinikoski, Antti J Kangas, Pasi Soininen, Hanna Lagström, Jorma S A Viikari, Tapani Rönnemaa, Antti Jula, Mika Ala-Korpela, Peter Würtz, Olli T Raitakari
OBJECTIVES: To study the effects of repeated, infancy-onset dietary counseling on a detailed metabolic profile. Effects of dietary saturated fat replacement on circulating concentrations of metabolic biomarkers still remain unknown. STUDY DESIGN: The Special Turku Coronary Risk Factor Intervention Project (STRIP) study is a longitudinal, randomized atherosclerosis prevention trial in which repeated dietary counseling aimed at reducing the proportion of saturated fat intake...
January 31, 2018: Journal of Pediatrics
https://www.readbyqxmd.com/read/29363944/structure-activity-relationship-studies-of-a-macrocyclic-agrp-mimetic-scaffold-c-pro-arg-phe-phe-asn-ala-phe-dpro-yield-potent-and-selective-melanocortin-4-receptor-antagonists-and-melanocortin-5-receptor-inverse-agonists-that-increase-food-intake-in-mice
#4
Katlyn A Fleming, Mark D Ericson, Katie T Freeman, Danielle N Adank, Mary M Lunzer, Stacey L Wilber, Carrie Haskell-Luevano
The melanocortin system has five receptors and antagonists of the central melanocortin receptors (MC3R, MC4R) are postulated to be viable therapeutics for disorders of negative energy balance such as anorexia, cachexia, and failure to thrive. Agouti-related protein (AGRP) is an antagonist of the MC3R and an antagonist/inverse agonist of the MC4R. Biophysical NMR based structural studies have demonstrated that the active sequence of this hormone, Arg-Phe-Phe, is located on an exposed β-hairpin loop. It has previously been demonstrated that the macrocyclic octapeptide scaffold c[Pro1-Arg2-Phe3-Phe4-Asn5-Ala6-Phe7-DPro8] is 16-fold less potent than AGRP at the mMC4R...
January 24, 2018: ACS Chemical Neuroscience
https://www.readbyqxmd.com/read/29260557/keikipukalides-furanocembrane-diterpenes-from-the-antarctic-deep-sea-octocoral-plumarella-delicatissima
#5
Santana A L Thomas, Jacqueline L von Salm, Shane Clark, Steve Ferlita, Prasanth Nemani, Ala Azhari, Christopher A Rice, Nerida G Wilson, Dennis E Kyle, Bill J Baker
During a 2013 cruise in the Southern Ocean we collected specimens of the octocoral Plumarella delicatissima between 800 and 950 m depth. Five new furanocembranoid diterpenes, keikipukalides A-E (1-5), the known diterpene pukalide aldehyde (6), and the known norditerpenoid ineleganolide (7) were isolated from the coral. These Plumarella terpenes lack mammalian cytotoxicity, while 2-7 display activity against Leishmania donovani between 1.9 and 12 μM. Structure elucidation was facilitated by one- and two-dimensional NMR spectroscopy and mass spectrometry, and keikipukalides A and E were confirmed by X-ray crystallography...
December 20, 2017: Journal of Natural Products
https://www.readbyqxmd.com/read/29226610/nuclear-magnetic-resonance-based-metabolomics-identifies-phenylalanine-as-a-novel-predictor-of-incident-heart-failure-hospitalisation-results-from-prosper-and-finrisk-1997
#6
Christian Delles, Naomi J Rankin, Charles Boachie, Alex McConnachie, Ian Ford, Antti Kangas, Pasi Soininen, Stella Trompet, Simon P Mooijaart, J Wouter Jukema, Faiez Zannad, Mika Ala-Korpela, Veikko Salomaa, Aki S Havulinna, Paul Welsh, Peter Würtz, Naveed Sattar
AIMS: We investigated the association between quantified metabolite, lipid and lipoprotein measures and incident heart failure hospitalisation (HFH) in the elderly, and examined whether circulating metabolic measures improve HFH prediction. METHODS AND RESULTS: Overall, 80 metabolic measures from the PROspective Study of Pravastatin in the Elderly at Risk (PROSPER) trial were measured by proton nuclear magnetic resonance spectroscopy (n = 5341; 182 HFH events during 2...
December 11, 2017: European Journal of Heart Failure
https://www.readbyqxmd.com/read/29190078/cu-ii-binding-to-the-peptide-ala-his-his-a-chimera-of-the-canonical-cu-ii-binding-motifs-xxx-his-and-xxx-zzz-his
#7
Paulina Gonzalez, Bertrand Vileno, Karolina Bossak, Youssef El Khoury, Petra Hellwig, Wojciech Bal, Christelle Hureau, Peter Faller
Peptides and proteins with the N-terminal motifs NH2-Xxx-His and NH2-Xxx-Zzz-His form well-established Cu(II) complexes. The canonical peptides are Gly-His-Lys and Asp-Ala-His-Lys (from the wound healing factor and human serum albumin, respectively). Cu(II) is bound to NH2-Xxx-His via three nitrogens from the peptide and an external ligand in the equatorial plane (called 3N form here). In contrast, Cu(II) is bound to NH2-Xxx-Zzz-His via four nitrogens from the peptide in the equatorial plane (called 4N form here)...
November 30, 2017: Inorganic Chemistry
https://www.readbyqxmd.com/read/29185935/dha-mediates-the-protective-effect-of-fish-consumption-on-new-episodes-of-depression-among-women
#8
John L Reeves, Petr Otahal, Costan G Magnussen, Terry Dwyer, Antti J Kangas, Pasi Soininen, Mika Ala-Korpela, Alison J Venn, Kylie J Smith
In a longitudinal cohort study of young Australian adults, we reported that for women higher baseline levels of fish consumption were associated with reduced incidence of new depressive episodes during the 5-year follow-up. Fish are high in both n-3 fatty acids and tyrosine. In this study, we seek to determine whether n-3 fatty acids or tyrosine explain the observed association. During 2004-2006, a FFQ (nine fish items) was used to estimate weekly fish consumption among 546 women aged 26-36 years. A fasting blood sample was taken and high-throughput NMR spectroscopy was used to measure 233 metabolites, including serum n-3 fatty acids and tyrosine...
November 2017: British Journal of Nutrition
https://www.readbyqxmd.com/read/28858505/synthesis-of-intrinsically-blue-colored-bis-nitronyl-nitroxide-peptidomimetic-templates-and-their-conformational-preferences-as-revealed-by-a-combined-spectroscopic-analysis
#9
Marta De Zotti, Karen Wright, Edouard d'Aboville, Antonio Toffoletti, Claudio Toniolo, Giovanna Longhi, Giuseppe Mazzeo, Sergio Abbate, Fernando Formaggio
The intrinsically blue-colored Ullman imidazolinyl nitronyl nitroxide (NN) mono-radicals have found various applications, in particular as spin probes and organic magnetic materials. Here, we present the solution-phase synthesis, extensive characterization, and conformational analysis of the first peptidomimetics with two pendant, chiral nitronyl nitroxide free radical units. Two (R)-Aic(NN) residues, where Aic(NN) is 2-amino-5-nitronylnitroxide-indan-2-carboxylic acid, have been inserted at positions i and i+3 of the pentapeptide Boc-(R)-Aic(NN)-(Ala)2-(R)-Aic(NN)-Ala-OMe and the hexapeptide Boc-[Ala-(R)-Aic(NN)-Ala]2-OMe as well...
September 18, 2017: Journal of Organic Chemistry
https://www.readbyqxmd.com/read/28856561/site-selective-13-c-labeling-of-histidine-and-tryptophan-using-ribose
#10
Ulrich Weininger
Experimental studies on protein dynamics at atomic resolution by NMR-spectroscopy in solution require isolated (1)H-X spin pairs. This is the default scenario in standard (1)H-(15)N backbone experiments. Side chain dynamic experiments, which allow to study specific local processes like proton-transfer, or tautomerization, require isolated (1)H-(13)C sites which must be produced by site-selective (13)C labeling. In the most general way this is achieved by using site-selectively (13)C-enriched glucose as the carbon source in bacterial expression systems...
September 2017: Journal of Biomolecular NMR
https://www.readbyqxmd.com/read/28846849/cyclic-hexapeptide-dimers-antatollamides-a-and-b-from-the-ascidian-didemnum-molle-a-tryptophan-derived-auxiliary-for-l-and-d-amino-acid-assignments
#11
Mariam N Salib, Tadeusz F Molinski
Two dimerized cyclic hexapeptides, antatollamides A (1) and B (2), were isolated from the colonial ascidian Didemnum molle collected in Pohnpei. The amino acid compositions and sequences were determined by interpretation of MS and 1D and 2D NMR data. Raney Ni reduction of antatollamide A cleaved the dimer to the corresponding monomeric cyclic hexapeptide with replacement of Cys by Ala. The amino acid configuration of 1 was established, after total hydrolysis, by derivatization with a new chiral reagent, (5-fluoro-2,4-dinitrophenyl)-N(α)-l-tryptophanamide (FDTA), prepared from l-Trp, followed by LCMS analysis; all amino acids were found to be l-configured except for d-Ala...
September 18, 2017: Journal of Organic Chemistry
https://www.readbyqxmd.com/read/28766333/monitoring-hydrogen-exchange-during-protein-folding-by-fast-pressure-jump-nmr-spectroscopy
#12
T Reid Alderson, Cyril Charlier, Dennis A Torchia, Philip Anfinrud, Ad Bax
A method is introduced that permits direct observation of the rates at which backbone amide hydrogens become protected from solvent exchange after rapidly dropping the hydrostatic pressure inside the NMR sample cell from denaturing (2.5 kbar) to native (1 bar) conditions. The method is demonstrated for a pressure-sensitized ubiquitin variant that contains two Val to Ala mutations. Increased protection against hydrogen exchange with solvent is monitored as a function of time during the folding process. Results for 53 backbone amides show narrow clustering with protection occurring with a time constant of ca...
August 16, 2017: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/28437077/peptide-and-protein-dynamics-and-low-temperature-dnp-magic-angle-spinning-nmr
#13
Qing Zhe Ni, Evgeny Markhasin, Thach V Can, Björn Corzilius, Kong Ooi Tan, Alexander B Barnes, Eugenio Daviso, Yongchao Su, Judith Herzfeld, Robert G Griffin
In DNP MAS NMR experiments at ∼80-110 K, the structurally important -(13)CH3 and -(15)NH3(+) signals in MAS spectra of biological samples disappear due to the interference of the molecular motions with the (1)H decoupling. Here we investigate the effect of these dynamic processes on the NMR line shapes and signal intensities in several typical systems: (1) microcrystalline APG, (2) membrane protein bR, (3) amyloid fibrils PI3-SH3, (4) monomeric alanine-CD3, and (5) the protonated and deuterated dipeptide N-Ac-VL over 78-300 K...
May 10, 2017: Journal of Physical Chemistry. B
https://www.readbyqxmd.com/read/27618034/glycerin-induced-conformational-changes-in-bombyx-mori-silk-fibroin-film-monitored-by-13-c-cp-mas-nmr-and-%C3%A2-h-dqmas-nmr
#14
Tetsuo Asakura, Masanori Endo, Misaki Hirayama, Hiroki Arai, Akihiro Aoki, Yugo Tasei
In order to improve the stiff and brittle characteristics of pure Bombyx mori (B. mori) silk fibroin (SF) film in the dry state, glycerin (Glyc) has been used as a plasticizer. However, there have been very limited studies on the structural characterization of the Glyc-blended SF film. In this study, (13)C Cross Polarization/Magic Angle Spinning nuclear magnetic resonance (CP/MAS NMR) was used to monitor the conformational changes in the films by changing the Glyc concentration. The presence of only 5 wt % Glyc in the film induced a significant conformational change in SF where Silk I* (repeated type II β-turn and no α-helix) newly appeared...
September 9, 2016: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/27605587/characterization-of-the-metabolic-profile-associated-with-serum-25-hydroxyvitamin-d-a-cross-sectional-analysis-in-population-based-data
#15
Susanne Vogt, Simone Wahl, Johannes Kettunen, Susanne Breitner, Gabi Kastenmüller, Christian Gieger, Karsten Suhre, Melanie Waldenberger, Jürgen Kratzsch, Markus Perola, Veikko Salomaa, Stefan Blankenberg, Tanja Zeller, Pasi Soininen, Antti J Kangas, Annette Peters, Harald Grallert, Mika Ala-Korpela, Barbara Thorand
BACKGROUND: Numerous observational studies have observed associations between vitamin D deficiency and cardiometabolic diseases, but these findings might be confounded by obesity. A characterization of the metabolic profile associated with serum 25-hydroxyvitamin D [25(OH)D] levels, in general and stratified by abdominal obesity, may help to untangle the relationship between vitamin D, obesity and cardiometabolic health. METHODS: Serum metabolomics measurements were obtained from a nuclear magnetic resonance spectroscopy (NMR)- and a mass spectrometry (MS)-based platform...
September 7, 2016: International Journal of Epidemiology
https://www.readbyqxmd.com/read/27440045/ionization-properties-of-histidine-residues-in-the-lipid-bilayer-membrane-environment
#16
Ashley N Martfeld, Denise V Greathouse, Roger E Koeppe
We address the critically important ionization properties of histidine side chains of membrane proteins, when exposed directly to lipid acyl chains within lipid bilayer membranes. The problem is important for addressing general principles that may underlie membrane protein function. To this end, we have employed a favorable host peptide framework provided by GWALP23 (acetyl-GGALW(5)LALALALALALALW(19)LAGA-amide). We inserted His residues into position 12 or 14 of GWALP23 (replacing either Leu(12) or Leu(14)) and incorporated specific [(2)H]Ala labels within the helical core sequence...
September 2, 2016: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/27398680/probing-the-binding-of-cu-2-ions-to-a-fragment-of-the-a%C3%AE-1-42-polypeptide-using-fluorescence-spectroscopy-isothermal-titration-calorimetry-and-molecular-dynamics-simulations
#17
Joanna Makowska, Krzysztof Żamojć, Dariusz Wyrzykowski, Wioletta Żmudzińska, Dorota Uber, Małgorzata Wierzbicka, Wiesław Wiczk, Lech Chmurzyński
Steady-state and time-resolved fluorescence quenching measurements supported by isothermal titration calorimetry (ITC) and molecular dynamics simulations (MD), with the NMR-derived restraints, were used to investigate the interactions of Cu(2+) ions with a fragment of the Aβ(1-42) polypeptide, Aβ(5-16) with the following sequence: Ac-Arg-His-Asp-Ser-Gly-Tyr-Glu-Val-His-His-Gln-Lys-NH2, denoted as HZ1. The studies presented in this paper, when compared with our previous results (Makowska et al., Spectrochim...
September 2016: Biophysical Chemistry
https://www.readbyqxmd.com/read/27278961/biotransformation-and-rearrangement-of-laromustine
#18
Alaa-Eldin F Nassar, Adam V Wisnewski, Ivan King
This review highlights the recent research into the biotransformations and rearrangement of the sulfonylhydrazine-alkylating agent laromustine. Incubation of [(14)C]laromustine with rat, dog, monkey, and human liver microsomes produced eight radioactive components (C-1 to C-8). There was little difference in the metabolite profile among the species examined, partly because NADPH was not required for the formation of most components, which instead involved decomposition and/or hydrolysis. The exception was C-7, a hydroxylated metabolite, largely formed by CYP2B6 and CYP3A4/5...
August 2016: Drug Metabolism and Disposition: the Biological Fate of Chemicals
https://www.readbyqxmd.com/read/27243469/the-carboxyl-terminus-of-eremomycin-facilitates-binding-to-the-non-d-ala-d-ala-segment-of-the-peptidoglycan-pentapeptide-stem
#19
James Chang, Hongyu Zhou, Maria Preobrazhenskaya, Peng Tao, Sung Joon Kim
Glycopeptide antibiotics inhibit cell wall biosynthesis in Gram-positive bacteria by targeting the peptidoglycan (PG) pentapeptide stem structure (l-Ala-d-iso-Gln-l-Lys-d-Ala-d-Ala). Structures of the glycopeptide complexed with a PG stem mimic have shown that the d-Ala-d-Ala segment is the primary drug binding site; however, biochemical evidence suggests that the glycopeptide-PG interaction involves more than d-Ala-d-Ala binding. Interactions of the glycopeptide with the non-d-Ala-d-Ala segment of the PG stem were investigated using solid-state nuclear magnetic resonance (NMR)...
June 21, 2016: Biochemistry
https://www.readbyqxmd.com/read/27226426/helix-nucleation-by-the-smallest-known-%C3%AE-helix-in-water
#20
Huy N Hoang, Russell W Driver, Renée L Beyer, Timothy A Hill, Aline D de Araujo, Fabien Plisson, Rosemary S Harrison, Lena Goedecke, Nicholas E Shepherd, David P Fairlie
Cyclic pentapeptides (e.g. Ac-(cyclo-1,5)-[KAXAD]-NH2 ; X=Ala, 1; Arg, 2) in water adopt one α-helical turn defined by three hydrogen bonds. NMR structure analysis reveals a slight distortion from α-helicity at the C-terminal aspartate caused by torsional restraints imposed by the K(i)-D(i+4) lactam bridge. To investigate this effect on helix nucleation, the more water-soluble 2 was appended to N-, C-, or both termini of a palindromic peptide ARAARAARA (≤5 % helicity), resulting in 67, 92, or 100 % relative α-helicity, as calculated from CD spectra...
July 11, 2016: Angewandte Chemie
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