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https://www.readbyqxmd.com/read/28340458/toxic-effects-of-environmental-pollutants-comparative-investigation-using-allium-cepa-l-and-lactuca-sativa-l
#1
Graciele Lurdes Silveira, Maria Gabriela Franco Lima, Gabriela Barreto Dos Reis, Marcel José Palmieri, Larissa Fonseca Andrade-Vieria
Studies that help understand the mechanisms of action of environmental pollutants are extremely important in environmental toxicology. In this context, assays using plants as models stand out for their simplicity and low performance cost. Among the plants used for this purpose, Allium cepa L. is the model most commonly applied for cytogenotoxic tests, while Lactuca sativa L., already widely used in phytotoxic investigations, has been gaining prominence in cytotoxic analyses. The present study aimed to compare the responses of A...
March 21, 2017: Chemosphere
https://www.readbyqxmd.com/read/28322131/soluble-flt-1-has-cytotoxic-effects-on-bewo-choriocarcinoma-cells
#2
Michiko Yamashita, Keiichi Kumasawa, Tatsuya Miyake, Hitomi Nakamura, Tadashi Kimura
BACKGROUND: Vascular endothelial growth factor (VEGF) plays a pivotal role in angiogenesis and is overexpressed in many kinds of malignant tumors. Soluble VEGF receptor 1 (sVEGFR-1/ soluble fms-like tyrosine kinase 1 [sFlt-1]) plays a role as an inhibitor of VEGF, and an antitumor effect has been shown in several studies using sFlt-1. Recently, in addition to its antiangiogenic effect, it was reported that sFlt-1 has direct cytotoxicity. MATERIALS AND METHODS: Transfection of sFlt-1 plasmid DNA was performed in the BeWo choriocarcinoma cell line...
January 1, 2017: Reproductive Sciences
https://www.readbyqxmd.com/read/28321092/refractory-double-hit-lymphoma-leukemia-in-childhood-mimicking-b-precursor-acute-lymphoblastic-leukemia-at-initial-presentation
#3
Suguru Uemura, Daiichiro Hasegawa, Takehito Yokoi, Nanako Nino, Teppei Tahara, Akihiro Tamura, Atsuro Saito, Aiko Kozaki, Kenji Kishimoto, Toshiaki Ishida, Keiichiro Kawasaki, Nobuyuki Yamamoto, Takeshi Mori, Noriyuki Nishimura, Yoshiyuki Kosaka
A 10-year-old girl was referred to our hospital with left preauricular adenopathy and gingival swelling. She was diagnosed with B-cell precursor acute lymphoblastic leukemia (BCP-ALL) based on being positive for expressions of CD10, CD19, TdT and HLA-DR. She showed no CD20 expression at the time of diagnosis. Based on the initial diagnosis of BCP-ALL, induction chemotherapy for BCP-ALL was initiated. However, the blasts did not disappear from her peripheral blood. Bone marrow examination on day 33 identified 81...
2017: [Rinshō Ketsueki] the Japanese Journal of Clinical Hematology
https://www.readbyqxmd.com/read/28319548/tanshinone-iia-attenuates-sevoflurane-neurotoxicity-in-neonatal-mice
#4
Yimeng Xia, Heng Xu, Chenfei Jia, Xiaodong Hu, Yu Kang, Xiaoxuan Yang, Qingsheng Xue, Guorong Tao, Buwei Yu
BACKGROUND: Sevoflurane is the most widely used inhalational anesthetic in pediatric medicine. Despite this, sevoflurane has been reported to exert potentially neurotoxic effects on the developing brain. Clinical interventions and treatments for these effects are limited. Tanshinone IIA (Tan IIA), extracted from Salvia miltiorrhiza (Danshen), has been documented to alleviate cognitive decline in traditional applications. Therefore, we hypothesized that preadministration of Tan IIA may attenuate sevoflurane-induced neurotoxicity, suggesting that Tan IIA is a new and promising drug capable of counteracting the effects of cognitive dysfunction produced by general anesthetics...
April 2017: Anesthesia and Analgesia
https://www.readbyqxmd.com/read/28300722/effect-of-5-aminolevulinic-acid-photodynamic-therapy-on-keratinocyte-proliferation-and-apoptosis-in-condyloma-acuminatum
#5
Guangwen Yin, Ke Sha, Bingjie Cai, Fangfang Li, Xuyang Li, Xianxian Xia, Xinxin Pan
BACKGROUND: The effect of 5-aminolevulinic acid photodynamic therapy on keratinocyte proliferation and apoptosis in condyloma acuminatum tissues was evaluated. METHODS: An immunohistochemical method and TdT-mediated dUTP nick end labeling were performed to detect the positive expression of the keratinocyte proliferation-related gene Ki-67 and apoptosis, respectively, in condyloma acuminatum tissues. RESULTS: Of 52 cases, 44 showed positive expression of Ki-67 in condyloma acuminatum keratinocytes before the treatment, with a positive expression rate of 84...
March 11, 2017: Photodiagnosis and Photodynamic Therapy
https://www.readbyqxmd.com/read/28282463/family-based-association-study-of-drd4-gene-in-methylphenidate-responded-attention-deficit-hyperactivity-disorder
#6
Patrick Wing-Leung Leung, Janice Ka Yan Chan, Lu Hua Chen, Chi Chiu Lee, Se Fong Hung, Ting Pong Ho, Chun Pan Tang, Robert K Moyzis, James M Swanson
The 48-basepair (48-bp) variable number tandem repeat (VNTR) polymorphism in exon 3 of the dopamine receptor D4 gene (DRD4) is implicated in the etiology of attention-deficit/ hyperactivity disorder (ADHD). In particular, ADHD in European-ancestry population is associated with an increased prevalence of the 7-repeat (7R) allele of the exon 3 VNTR. However, it is intriguing to note that the 7R allele has been found to be of very low prevalence in the Chinese general population. In a previous case-control study, our research team had found that the 7R allele was similarly absent in Chinese ADHD children in Hong Kong...
2017: PloS One
https://www.readbyqxmd.com/read/28264914/unique-spectral-signatures-of-the-nucleic-acid-dye-acridine-orange-can-distinguish-cell-death-by-apoptosis-and-necroptosis
#7
Jason R Plemel, Andrew V Caprariello, Michael B Keough, Tyler J Henry, Shigeki Tsutsui, Tak H Chu, Geert J Schenk, Roel Klaver, V Wee Yong, Peter K Stys
Cellular injury and death are ubiquitous features of disease, yet tools to detect them are limited and insensitive to subtle pathological changes. Acridine orange (AO), a nucleic acid dye with unique spectral properties, enables real-time measurement of RNA and DNA as proxies for cell viability during exposure to various noxious stimuli. This tool illuminates spectral signatures unique to various modes of cell death, such as cells undergoing apoptosis versus necrosis/necroptosis. This new approach also shows that cellular RNA decreases during necrotic, necroptotic, and apoptotic cell death caused by demyelinating, ischemic, and traumatic injuries, implying its involvement in a wide spectrum of tissue pathologies...
March 6, 2017: Journal of Cell Biology
https://www.readbyqxmd.com/read/28256626/treatment-of-steroid-induced-osteonecrosis-of-the-femoral-head-using-porous-se-sio2-nanocomposites-to-suppress-reactive-oxygen-species
#8
Guoying Deng, Kerun Niu, Feng Zhou, Buxiao Li, Yingjie Kang, Xijian Liu, Junqing Hu, Bo Li, Qiugen Wang, Chengqing Yi, Qian Wang
Reducing oxidative stress (ROS) have been demonstrated effective for steroid-induced osteonecrosis of the femoral head (steroid-induced ONFH). Selenium (Se) plays an important role in suppressing oxidative stress and has huge potential in ONFH treatments. However the Se has a narrow margin between beneficial and toxic effects which make it hard for therapy use in vivo. In order to make the deficiency up, a control release of Se (Se@SiO2) were realized by nanotechnology modification. Porous Se@SiO2 nanocomposites have favorable biocompatibility and can reduced the ROS damage effectively...
March 3, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28248816/tdt-positive-infiltrate-in-inflamed-pediatric-kidney-a-potential-diagnostic-pitfall
#9
Jennifer B Dunlap, Michael J Cascio, Xavier Stacey, Sarah Click, Megan L Troxell
We encountered a patient with infantile nephrotic syndrome associated with a dense interstitial inflammatory infiltrate and prominent extramedullary hematopoiesis. Immunohistochemical analysis revealed numerous terminal deoxynucleotidyl transferase (TdT)-positive cells, which may raise concern for lymphoblastic lymphoma. Thus, we further characterized a group of pediatric kidneys with inflammation. TdT-positive nuclei were quantitated, and dual immunostains for TdT/CD79a, TdT/CD3, and TdT/CD43 were performed in a subset of cases; flow cytometry was performed in 1 case...
February 28, 2017: American Journal of Surgical Pathology
https://www.readbyqxmd.com/read/28245344/inter-and-intra-laboratory-standardization-of-tunel-assay-for-assessment-of-sperm-dna-fragmentation
#10
S Ribeiro, R Sharma, S Gupta, Z Cakar, C De Geyter, A Agarwal
One of the challenges with the sperm DNA fragmentation results is the inconsistency and the large variability in the results obtained by different techniques. The terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay quantifies the incorporation of fluoresceinated dUTP into single- and double-strand DNA breaks by labeling the 3'-OH terminal with TdT. The goal of this study was optimize the TUNEL protocol for assessment of sperm DNA fragmentation by standardization of the method and comparison of the data across two reference laboratories (i) at Basel, Switzerland and (ii) Cleveland Clinic, Ohio, USA...
February 28, 2017: Andrology
https://www.readbyqxmd.com/read/28244454/applicability-of-a-single-5-color-cytoplasmic-markers-tube-as-primary-panel-for-immunophenotyping-of-acute-leukemia-a-gujarat-cancer-and-research-institute-experience
#11
B P Parikh, S P Patel, B N Raiya, H H Vora, D H Jetly
INTRODUCTION: Flow cytometry is highly sensitive for detection and quantitative analysis of surface and intracellular antigens in malignant hemopoietic cells. Immunophenotyping is a routine practice for classification and lineage assignment of acute leukemia. In the present study, our aim is to identify the role of a single 5 color, CD45, myeloperoxidase (MPO), cCD79a, cCD3, and Tdt, cytoplasmic markers combination as a primary tube. We compared with final diagnosis on the basis of morphology, cytochemistry, and primary and secondary panels of immunophenotyping and also with other study...
July 2016: Indian Journal of Cancer
https://www.readbyqxmd.com/read/28221267/health-related-quality-of-life-and-its-predictors-among-transfusion-dependent-thalassemia-patients
#12
Gulen Tuysuz, Funda Tayfun
PURPOSE: With regular blood transfusions and modern medical management strategies, transfusion-dependent thalassemia (TDT) patients are currently living into adulthood, but they still suffer from the chronicity of the disease and its complications. This study was aimed to assess the health-related quality of life (HRQoL) scores in pediatric TDT patients compared with healthy controls. PATIENTS AND METHODS: The PedsQL 4.0 Generic Core Scales were administered to 80 pediatric TDT patients (aged 5 to 18 y, child self-report) and their parents (parent-proxy report), as well as age-matched and sex-matched healthy controls (80 participants and their parents)...
February 17, 2017: Journal of Pediatric Hematology/oncology
https://www.readbyqxmd.com/read/28208281/enzyme-activated-g-quadruplex-synthesis-for-in-situ-label-free-detection-and-bioimaging-of-cell-apoptosis
#13
Zhuoliang Liu, Xingyu Luo, Zhu Li, Yan Huang, Zhou Nie, Hong-Hui Wang, Shouzhuo Yao
Fluorogenic probes targeting G-quadruplex structures have emerged as the promising toolkit for functional research of G-quadruplex and biosensor development. However, their biosensing applications are still largely limited in in-tube detection. Herein, we proposed a fluorescent bioimaging method based on enzyme-generated G-quadruplexes for detecting apoptotic cells at the cell and tissue level, namely, terminal deoxynucleotidyl transferase (TdT)-activated de novo G-quadruplex synthesis (TAGS) assay. The detection target is genomic DNA fragmentation, a biochemical hallmark of apoptosis...
February 7, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28202950/including-non-informative-parents-in-transmission-based-association-tests
#14
Hemant Kulkarni, Saurabh Ghosh
The classical transmission disequilibrium test (TDT) based on a trio design uses information only on the allele transmitted by a heterozygous parent at a marker locus as homozygous parents are non-informative about linkage. However, the phenotype of an offspring depends on the alleles transmitted by both parents, irrespective of whether the parents are homozygous or heterozygous, and hence carry useful information on association. In this article, we propose modifications to the TDT procedures by incorporating transmission data on both parents in an informative trio to explore possible gain in power in detecting association...
February 16, 2017: Journal of Human Genetics
https://www.readbyqxmd.com/read/28190861/acute-undifferentiated-leukemia-stem-cell-acute-leukemia-showing-differentiation-to-langerhans-cell-like-cells-in-lymph-nodes
#15
Takanori Fukuta, Shu Nakamoto, Yoshinori Hashimoto, Takayuki Tanaka, Yusuke Tokuyasu, Junko Maruyama, Hiromi Omura, Norihiko Hino, Satoshi Kuwamoto, Ichiro Murakami
A 75-year-old woman was referred to our hospital with suspected leukemia. Complete blood count demonstrated WBC 3,810/µl with 26% blasts, Hb of 11.7 g/dl and Plt of 18.0×10(4)/µl. Bone marrow aspiration revealed blasts (86.3%) with expressions of CD34, CD7, TdT, CD33, and CD117. MPO was negative. Chromosomal analysis of the bone marrow showed isolated trisomy 10 in all leukemic cells (20/20). Swelling of superficial lymph nodes was also observed. Cervical lymph node biopsy revealed leukemic blasts which had the same phenotype as those in the bone marrow except that proliferation of Langerhans cell-like cells (LCs) was observed in the paracortex...
2017: [Rinshō Ketsueki] the Japanese Journal of Clinical Hematology
https://www.readbyqxmd.com/read/28185128/effects-of-trpm7-mir-34a-gene-silencing-on-spatial-cognitive-function-and-hippocampal-neurogenesis-in-mice-with-type-1-diabetes-mellitus
#16
Qing-Jiu Zhang, Jie Li, Song-Yun Zhang
This study aimed to explore the effects of transient receptor potential melastatin 7 (TRPM7)/microRNA-34a (miR-34a) gene silencing on spatial cognitive function and hippocampal neurogenesis in mice with type 1 diabetes mellitus (T1DM). BALB/c mice were chosen to establish T1DM models and divided into five groups respectively: the negative control (NC), T1DM, T1DM + si-TRPM7, T1DM + si-miR-34a, and T1DM + si-TRPM7 + si-miR-34a groups. Morris water maze (MWM) test was adopted to observe behavioral alterations in mice...
February 9, 2017: Molecular Neurobiology
https://www.readbyqxmd.com/read/28165662/flow-cytometric-characteristics-of-extrathymic-thymocytes-in-adenoid-tissue-a-case-report-and-comparison-to-normal-thymus-and-thymoma
#17
Ji Yuan, Vasantha L Gali, Deborah A Perry, Kai Fu, Hina Qureishi, Catalina Amador-Ortiz, Timothy Greiner, Samuel J Pirruccello
BACKGROUND: Normal thymocyte precursors in secondary lymphoid organs have previously been described. It is important to recognize normal thymocyte precursors by flow cytometry to differentiate them from T-cell lymphoblastic leukemia. METHODS: A 3-year-old boy status 2 years post-allogenic cardiac transplant underwent adenoidectomy to exclude post-transplant lymphoproliferative disorder. Microscopic, immunohistochemical, and flow cytometry analyses of the adenoid were performed...
February 6, 2017: Cytometry. Part B, Clinical Cytometry
https://www.readbyqxmd.com/read/28165366/lychee-seed-saponins-improve-cognitive-function-and-prevent-neuronal-injury-via-inhibiting-neuronal-apoptosis-in-a-rat-model-of-alzheimer-s-disease
#18
Xiuling Wang, Jianming Wu, Chonglin Yu, Yong Tang, Jian Liu, Haixia Chen, Bingjin Jin, Qibing Mei, Shousong Cao, Dalian Qin
Lychee seed is a traditional Chinese medicine and possesses many activities, including hypoglycemia, liver protection, antioxidation, antivirus, and antitumor. However, its effect on neuroprotection is still unclear. The present study investigated the effects of lychee seed saponins (LSS) on neuroprotection and associated mechanisms. We established a rat model of Alzheimer's disease (AD) by injecting Aβ25-35 into the lateral ventricle of rats and evaluated the effect of LSS on spatial learning and memory ability via the Morris water maze...
February 4, 2017: Nutrients
https://www.readbyqxmd.com/read/28163115/jia-jian-di-huang-yin-zi-decoction-reduces-apoptosis-induced-by-both-mitochondrial-and-endoplasmic-reticulum-caspase12-pathways-in-the-mouse-model-of-parkinson-s-disease
#19
Jingsi Zhang, Zhennian Zhang, Jie Bao, Zhonghai Yu, Min Cai, Xiangting Li, Ting Wu, Jun Xiang, Dingfang Cai
ETHNOPHARMACOLOGICAL RELEVANCE: As a classical prescription of traditional Chinese medicine (TCM), Jia-Jian-Di-Huang-Yin-Zi decoction (JJDHYZ) has been used to treat the symptoms of neurological disorders with a long history. AIM OF THE STUDY: To evaluate the effects and possible mechanisms of JJDHYZ on a 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-induced subacute mouse model of Parkinson's disease. MATERIALS AND METHODS: Adult male C57BL/6 mice were randomly divided into five groups: control, MPTP, JJDHYZ low dosage (JJDHYZ-L, 8...
February 2, 2017: Journal of Ethnopharmacology
https://www.readbyqxmd.com/read/28160584/new-insights-from-gwas-for-the-cleft-palate-among-han-chinese-population
#20
S-J Duan, N Huang, B-H Zhang, J-Y Shi, S He, J Ma, Q-Q Yu, B Shi, Z-L Jia
BACKGROUND: Genome wide association studies (GWAS) already have identified tens of susceptible loci for nonsyndromic cleft lip with or without cleft palate (NSCL/P). However, whether these loci associated with nonsyndromic cleft palate only (NSCPO) remains unknown. MATERIAL AND METHODS: In this study, we replicated 38 SNPs (Single nucleotide polymorphisms) which has the most significant p values in published GWASs, genotyping by using SNPscan among 144 NSCPO trios from Western Han Chinese...
March 1, 2017: Medicina Oral, Patología Oral y Cirugía Bucal
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