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https://www.readbyqxmd.com/read/28544921/in-situ-formed-copper-nanoparticles-templated-by-tdt-mediated-dna-for-enhanced-spr-sensor-based-dna-assay
#1
Pei-Xin Yuan, Sheng-Yuan Deng, Chen-Yu Zheng, Serge Cosnier, Dan Shan
For the efficient surface plasmon resonance (SPR)-based DNA assay researching, signal amplification tactics were absolutely necessary. In this work, a sensitive SPR-DNA sensor was developed by employing in situ synthesis of copper nanoparticles (CuNPs) templated by poly-T sequences DNA from terminal deoxynucleotidyl transferase (TdT)-mediated extension, and synergistically with nano-effect deposition as the mass relay. The objective of this strategy was manifold: firstly, tDNA hybridized with the optimal designed probes to active the TdT-mediated DNA extension onto the surface of SPR chip, resulted a long poly-T sequences ssDNA chain in dsDNA terminal onto surface of gold chip and characterized by SPR signal amplitudes...
May 18, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28544409/mind-the-gap-temporal-discrimination-and-dystonia
#2
A Sadnicka, C Daum, C Cordivari, K P Bhatia, J C Rothwell, S Manohar, M J Edwards
BACKGROUND AND PURPOSE: One of the most widely studied perceptual measures of sensory dysfunction in dystonia is the temporal discrimination threshold (TDT) (the shortest interval at which subjects can perceive that there are two stimuli rather than one). However the elevated thresholds described may be due to a number of potential mechanisms as current paradigms test not only temporal discrimination but also extraneous sensory and decision-making parameters. In this study two paradigms designed to better quantify temporal processing are presented and a decision-making model is used to assess the influence of decision strategy...
June 2017: European Journal of Neurology: the Official Journal of the European Federation of Neurological Societies
https://www.readbyqxmd.com/read/28531803/isofraxidin-inhibited-proliferation-and-induced-apoptosis-via-blockage-of-akt-pathway-in-human-colorectal-cancer-cells
#3
Peng Shen, Hong-Gang Wang, Miao-Miao Li, Qian-Yun Ma, Chuan-Wen Zhou, Feng Pan, Rui Xie
BACKGROUND: Isofraxidin (IF), a natural coumarin compound, has been reported to possess anti-cancer activity in human liver cancer. However, whether IF is involved in the regulation of colorectal cancer tumorigenesis and development has been not well elucidated. METHODS: The cell proliferation were assessed by Cell Counting Kit-8 (CCK-8) and colony formation test, respectively. The transwell assays were conducted to estimate cell migration and invasion abilities...
May 19, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28513609/application-of-rare-variant-transmission-disequilibrium-tests-to-epileptic-encephalopathy-trio-sequence-data
#4
(no author information available yet)
The classic epileptic encephalopathies, including infantile spasms (IS) and Lennox-Gastaut syndrome (LGS), are severe seizure disorders that usually arise sporadically. De novo variants in genes mainly encoding ion channel and synaptic proteins have been found to account for over 15% of patients with IS or LGS. The contribution of autosomal recessive genetic variation, however, is less well understood. We implemented a rare variant transmission disequilibrium test (TDT) to search for autosomal recessive epileptic encephalopathy genes in a cohort of 320 outbred patient-parent trios that were generally prescreened for rare metabolic disorders...
May 17, 2017: European Journal of Human Genetics: EJHG
https://www.readbyqxmd.com/read/28502878/the-relationship-between-sensory-loss-and-persistent-pain-1-year-after-breast-cancer-surgery
#5
Kenneth Geving Andersen, Helle Molter Duriaud, Henrik Kehlet, Eske Kvanner Aasvang
Moderate-to-severe persistent pain after breast cancer surgery (PPBCS) affects 10-20% of the patients. Sensory dysfunction is often concomitantly present suggesting a neuropathic pain state. The relationship between various postoperative pain states and sensory dysfunction has been examined by quantitative sensory testing (QST), but only 2 smaller studies have examined PPBCS and sensory dysfunction in the surgical area. The purpose of this prospective study was to assess the relative importance of sensory function and PPBCS...
May 11, 2017: Journal of Pain: Official Journal of the American Pain Society
https://www.readbyqxmd.com/read/28501723/tangeretin-alters-neuronal-apoptosis-and-ameliorates-the-severity-of-seizures-in-experimental-epilepsy-induced-rats-by-modulating-apoptotic-protein-expressions-regulating-matrix-metalloproteinases-and-activating-the-pi3k-akt-cell-survival-pathway
#6
Xiao-Qian Guo, Yu-Ling Cao, Fang Hao, Zhong-Rui Yan, Mei-Ling Wang, Xue-Wu Liu
PURPOSE: Epilepsy is complex neural disarray categorized by recurring seizures. Despite recent advances in pharmacotherapies for epilepsy, its treatment remains a challenge due to the contrary effects of the drugs. As a result, the identification of novel anti-epileptic drugs (AEDs) with neuroprotective properties and few side effects is of great value. Thus, the present study assessed the treatment effects of tangeretin using a rat model of pilocarpine-induced epilepsy. MATERIALS AND METHODS: Separate groups of male Wistar rats received oral administrations of tangeretin at 50, 100, or 200mg/kg for 10 days and then, on the 10th day, they received an intraperitoneal injection of pilocarpine (30mg/kg)...
May 11, 2017: Advances in Medical Sciences
https://www.readbyqxmd.com/read/28499404/anterograde-monosynaptic-transneuronal-tracers-derived-from-herpes-simplex-virus-1-strain-h129
#7
Wen-Bo Zeng, Hai-Fei Jiang, Ya-Dong Gang, Yi-Ge Song, Zhang-Zhou Shen, Hong Yang, Xiao Dong, Yong-Lu Tian, Rong-Jun Ni, Yaping Liu, Na Tang, Xinyan Li, Xuan Jiang, Ding Gao, Michelle Androulakis, Xiao-Bin He, Hui-Min Xia, Ying-Zi Ming, Youming Lu, Jiang-Ning Zhou, Chen Zhang, Xue-Shan Xia, Yousheng Shu, Shao-Qun Zeng, Fuqiang Xu, Fei Zhao, Min-Hua Luo
BACKGROUND: Herpes simplex virus type 1 strain 129 (H129) has represented a promising anterograde neuronal circuit tracing tool, which complements the existing retrograde tracers. However, the current H129 derived tracers are multisynaptic, neither bright enough to label the details of neurons nor capable of determining direct projection targets as monosynaptic tracer. METHODS: Based on the bacterial artificial chromosome of H129, we have generated a serial of recombinant viruses for neuronal circuit tracing...
May 12, 2017: Molecular Neurodegeneration
https://www.readbyqxmd.com/read/28498400/trim28-knockdown-increases-sensitivity-to-etoposide-by-upregulating-e2f1-in-non-small-cell-lung-cancer
#8
Lei Liu, Lijun Xiao, Xiujun Liang, Long Chen, Luyang Cheng, Lei Zhang, Xiaoguang Wu, Qian Xu, Chunhu Ma
Tripartite motif containing 28 (TRIM28) is a universal corepressor for Kruppel‑associated box zinc finger proteins. In our previous study, it was shown that expression of TRIM28 is upregulated in non‑small cell lung cancer (NSCLC) cell lines and tissues. Here, we demonstrated that the stable silencing of TRIM28 expression by a specific siRNA lentivirus vector increased the sensitivity of NSCLC cells to chemotherapeutic agent etoposide. Combination of TRIM28 siRNA and etoposide significantly inhibited the growth and proliferation of lung adenocarcinoma PAa cells and exerted obvious antitumor effects in nude mice...
May 11, 2017: Oncology Reports
https://www.readbyqxmd.com/read/28488007/effects-of-rat-anti-mouse-interleukin-6-receptor-antibody-on-the-recovery-of-cognitive-function-in-stroke-mice
#9
Jun Wei, Chongling Sun, Chang Liu, Qimei Zhang
This study aimed to investigate the effects of rat anti-mouse interleukin (IL)-6 receptor antibody (MR16-1) on the recovery of cognitive function in stroke mice. Adult male C57BL/6 mice were subjected to middle cerebral artery occlusion (MCAO). Mice were randomly assigned into three groups: sham group, model group, and MR16-1 group. After the treatment of MR16-1, spatial learning and memory performance of mice were evaluated by the Morris water maze (MWM) and Y-maze tests. Then, brain slices were obtained and infarct volume and neuronal apoptosis were assessed by 2,3,5-triphenyltetrazolium chloride (TTC) staining and Terminal deoxynucleotidyl transferase (TdT)-mediated dUTP nick end labeling (TUNEL) assay, respectively...
May 9, 2017: Cellular and Molecular Neurobiology
https://www.readbyqxmd.com/read/28470785/formation-of-group%C3%A2-11-bismuth-sulfide-nanoparticles-using-bismuth-dithioates-under-mild-conditions
#10
Dimuthu C Senevirathna, Melissa V Werrett, Narendra Pai, Victoria L Blair, Leone Spiccia, Philip C Andrews
The formation of mixed-metal sulfides with the general structure AgBiS2 and Cu3 BiS2 by a simple two-step process utilizing bismuth dithiocarboxylates as Bi and S precursors is described. A sonochemical reaction of Bi2 O3 with six different aryl dithioic acids: dithiobenzoic acid (BDT-H), 4-methoxydithiobenzoic acid (4-MBDT-H), 3-methyldithiobenzoic acid (3-MBDT-H), 2-mesitylenedithioic acid (2-MDT-H), 4-fluorodithiobenzoic acid (4-FBDT-H), and 2-thiophenedithioic acid (2-TDT-H) resulted in the corresponding complexes: [Bi(BDT)3 ] 1, [Bi(4-MBDT)3 ] 2, [{Bi(3-MBDT)3 }2 ⋅C7 H8 ] (32 ⋅C7 H8 ), [Bi(2-MDT)3 ] 4, [Bi(4-FBDT)3 ] 5 and [Bi(2-TDT)3 ] 6...
May 3, 2017: Chemistry: a European Journal
https://www.readbyqxmd.com/read/28470345/lncrna-ccat1-inhibits-cell-apoptosis-of-renal-cell-carcinoma-through-up-regulation-of-livin-protein
#11
Shaoan Chen, Pengpeng Ma, Bin Li, Dawei Zhu, Xiude Chen, Yuzhu Xiang, Tengteng Wang, Xiangbin Ren, Chuan Liu, Xunbo Jin
This study was to investigate the involvement of long non-coding RNA (lncRNA) colon cancer-associated transcript-1 (CCAT1) in renal cell carcinoma (RCC) and to further uncover its underlying mechanism. In this study, the expression of CCAT1 and Livin of RCC tissues or cells was determined using qRT-PCR (quantitative real-time PCR) and western blot, respectively. RNA pulldown and RIP (RNA-Binding Protein Immunoprecipitation) assays were performed to examine the sequence interaction between CCAT1 and Livin. The viability and apoptosis of RCC cells was assessed by MTT(3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) and TUNEL (TdT-mediated dUTP nick end labeling) assays, respectively...
May 3, 2017: Molecular and Cellular Biochemistry
https://www.readbyqxmd.com/read/28469655/electroacupuncture-reduces-apoptotic-index-and-inhibits-p38-mitogen-activated-protein-kinase-signaling-pathway-in-the-hippocampus-of-rats-with-cerebral-ischemia-reperfusion-injury
#12
Xiao Lan, Xin Zhang, Guo-Ping Zhou, Chun-Xiao Wu, Chun Li, Xiu-Hong Xu
Electroacupuncture attenuates cerebral hypoxia and neuronal apoptosis induced by cerebral ischemia/reperfusion injury. To further identify the involved mechanisms, we assumed that electroacupuncture used to treat cerebral ischemia/reperfusion injury was associated with the p38 mitogen-activated protein kinase (MAPK) signaling pathway. We established rat models of cerebral ischemia/reperfusion injury using the modified Zea-Longa's method. At 30 minutes before model establishment, p38 MAPK blocker SB20358 was injected into the left lateral ventricles...
March 2017: Neural Regeneration Research
https://www.readbyqxmd.com/read/28468965/cell-specific-qrt-pcr-of-renal-epithelial-cells-reveals-a-novel-innate-immune-signature-in-murine-collecting-duct
#13
Vijay Saxena, David S Hains, John Ketz, Melinda Chanley, John D Spencer, Brian Becknell, Keith R Pierce, Raoul D Nelson, Jeffrey M Purkerson, George J Schwartz, Andrew L Schwaderer
The urinary tract is usually culture negative despite its close proximity to microbial flora. The precise mechanisms by which the kidneys and urinary tract defends against infection is not well understood. The initial kidney cells to encounter ascending pathogens are the collecting tubule cells which consist of principal cells (PCs) that express aquaporin 2 (AQP2) and intercalated cells (ICs) that express vacuolar H+-ATPase (V-ATPase, B1 subunit). We have previously shown that ICs are involved with the human renal innate immune defense...
May 3, 2017: American Journal of Physiology. Renal Physiology
https://www.readbyqxmd.com/read/28468036/-clinicopathologic-features-of-atypical-type-a-thymoma
#14
Y C Su, J X Di, J P Da
Objective: To study the clinicopathologic features, immunophenotype and differential diagnosis of atypical type A thymoma. Methods: Clinicopathologic and follow-up data of three cases of atypical type A thymoma from 2004 to 2016 were reviewed. Immunohistochemical staining was performed. Results: All three patients were male with average age of 59 years. Clinically, the lesions presented as anterior mediastinal masses. Grossly, the tumor ranged in size from 4 to 6 cm in greatest dimension and partially enclosed within fibrous capsule...
May 8, 2017: Zhonghua Bing Li Xue za Zhi Chinese Journal of Pathology
https://www.readbyqxmd.com/read/28467977/treatment-with-enriched-environment-reduces-neuronal-apoptosis-in-the-periinfarct-cortex-after-cerebral-ischemia-reperfusion-injury
#15
Xiuping Chen, Xin Zhang, Li Xue, Chizi Hao, Weijing Liao, Qi Wan
BACKGROUND/AIMS: Enriched environment (EE) has been reported to exert neuroprotective effect in animal models of ischemic stroke. However, the underlying mechanism remains unclear. The purpose of this study was to investigate the effect of EE treatment on neuronal apoptosis in the periinfarct cortex after cerebral ischemia/reperfusion (I/R) injury. METHODS: The cerebral I/R injury was established by middle cerebral artery occlusion (MCAO). A set of behavioral tests including the modified neurological severity score (mNSS), limb-placing test and foot-fault test were conducted...
2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/28466095/survival-of-the-fetus-fetal-b-and-t-cell-receptor-repertoire-development
#16
REVIEW
Erez Rechavi, Raz Somech
A mature and diverse T and B cell receptor repertoire is a prerequisite for immunocompetence. In light of its increased susceptibility to infection, the human fetus has long been considered deficient in this regard. However, data accumulated since the 1990s and in earnest in the past couple of years paints a more complicated picture. As we describe in this review, mechanisms responsible for generating a diverse receptor repertoire, such as somatic recombination, class switch recombination, and somatic hypermutation, are all operational to surprising extents in the growing fetus...
May 2, 2017: Seminars in Immunopathology
https://www.readbyqxmd.com/read/28459703/novel-cytogenetic-findings-in-a-case-of-mixed-phenotype-acute-leukemia-within-the-context-of-a-complex-karyotype
#17
David Shabsovich, Gary Schiller, Yalda Naeini, Robert Collins, Carlos A Tirado
BACKGROUND: Mixed phenotype acute leukemia (MPAL) is a rare hematological malignancy characterized by combinatorial aberrations involving cells of the myeloid, T-, and/or B- lineages, most often diagnosed by means of immunophenotyping in order to assess lineage-specific markers, which can still yield inconclusive diagnoses. MPAL with a complex karyotype (three or more chromosomal abnormalities) is a cytogenetic subtype of MPAL associated with a poor prognosis, but limited data is available about the cytogenetic abnormalities present in this context...
2017: Journal of the Association of Genetic Technologists
https://www.readbyqxmd.com/read/28459399/-biliverdin-protects-the-isolated-lungs-from-ischemia-reperfusion-injury-via-anti-apoptosis
#18
Jieyun Sun, Peng Zhang, Xinying Yang, Di Xu, Bin Du, Junliang Chen, Minchen Wu, Qingfeng Pang
OBJECTIVE: To observe the effect of biliverdin (BV) on the lung ischemia/reperfusion injury (LIRI), and to investigate the mechanism of BV in treatment of LIRI. METHODS: Thirty-two male Sprague-Dawley (SD) rats were randomly divided into control group, LIRI group, glutathione (GSH) group and BV-treated group, with 8 rats in each group. The rat LIRI model was reproduced by isolated lung perfusion system. Fifteen minutes after perfusion balance, lungs of control group were perfused continuously for 90 minutes, and the lungs in other groups were reperfused for 90 minutes after 1-hour ischemia, while the perfusion was added 10 ?mol/L BV in BV-treated group and 4 mmol/L GSH in GSH group respectively...
January 2017: Zhonghua Wei Zhong Bing Ji Jiu Yi Xue
https://www.readbyqxmd.com/read/28458020/signaling-by-growth-differentiation-factor-5-through-the-bone-morphogenetic-protein-receptor-type-ib-protects-neurons-against-kainic-acid-induced-neurodegeneration
#19
Yuanzheng Zhao, Min Zhang, Hengfang Liu, Jianping Wang
Growth/differentiation factor-5 (GDF-5), a member of the transforming growth factor-beta (TGF-β) superfamily, has been shown to protect rat dopaminergic neurons against insult both in embryonic neuronal culture and in Parkinson's disease models. However, whether GDF-5 exerts neuroprotective effects in hippocampal neurons is unclear. Here, we show that both mRNA levels and protein levels of GDF-5 are decreased in the mouse hippocampus upon kainic acid (KA) treatment. KA induced dramatic neuronal loss specifically in the cornu ammonis 1 (CA1) and CA3 areas of the mouse hippocampus, while intracerebral infusion of GDF-5 prevented this neuronal loss...
April 27, 2017: Neuroscience Letters
https://www.readbyqxmd.com/read/28454103/subchronic-arsenism-induced-oxidative-stress-and-inflammatory-contribute-to-apoptosis-through-mitochondrial-and-death-receptor-dependent-pathways-in-chicken-immune-organs
#20
Hongjing Zhao, Ying He, Siwen Li, Xiao Sun, Yu Wang, Yizhi Shao, Zhijun Hou, Mingwei Xing
In many organ dysfunctions, arsenic and its compounds are well known to induce apoptosis by the mitochondria and death receptor apoptotic pathways in liver and airway. However, it is less reported that which signaling pathways contribute to excessive apoptosis of chicken immune organs, a major target of toxic metals biotransformation, which suffer from subchronic arsenism. In this study, we investigated whether the mitochondria or death receptor apoptotic pathways activated in the immune organs (spleen, thymus and bursa of Fabricius) of one-day-old male Hy-line chickens exposed to arsenic trioxide (As2O3), which were fed on diets supplemented with 0, 0...
April 8, 2017: Oncotarget
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