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https://www.readbyqxmd.com/read/28809055/apoptotic-effect-of-astragalin-in-melanoma-skin-cancers-via-activation-of-caspases-and-inhibition-of-sry-related-hmg-box-gene-10
#1
Ok Heui You, Eun Ah Shin, Hyemin Lee, Ju-Ha Kim, Deok Yong Sim, Jung Hyo Kim, Younghwan Kim, Jae-Ho Khil, Nam-In Baek, Sung-Hoon Kim
Though Astragalin (kaempferol-3-glucoside) contained in Paeonia lactiflora and other plants was known to have anti-oxidant, antiinflammatory, and anti-tumor activity, the anti-tumor mechanism of Astragalin has never been reported in melanomas until now. Thus, in the present study, the underlying apoptotic mechanism of Astragalin isolated from Aceriphyllum rossii was elucidated in A375P and SK-MEL-2 melanoma cells. Astragalin exerted cytotoxicity in A375P and SK-MEL-2 cells in a concentration-dependent manner...
August 15, 2017: Phytotherapy Research: PTR
https://www.readbyqxmd.com/read/28808378/rutamarin-an-active-constituent-from-ruta-angustifolia-pers-induced-apoptotic-cell-death-in-the-ht29-colon-adenocarcinoma-cell-line
#2
Shafinah Ahmad Suhaimi, Sok Lai Hong, Sri Nurestri Abdul Malek
BACKGROUND: Ruta angustifolia Pers. is a perennial herb that is cultivated worldwide, including Southeast Asia, for the treatment of various diseases as traditional medicine. OBJECTIVE: The purpose of the study was to identify an active principle of R. angustifolia and to investigate its effect on the HT29 cell death. MATERIALS AND METHODS: The methanol and fractionated extracts (hexane, chloroform, ethyl acetate, and water) of R. angustifolia Pers...
July 2017: Pharmacognosy Magazine
https://www.readbyqxmd.com/read/28802359/transmission-analysis-of-tgfb1-gene-polymorphisms-in-non-syndromic-cleft-lip-with-or-without-cleft-palate
#3
Ginila T Raju, Bhaskar V K S Lakkakula, Jyotsna Murthy, Munirajan Arasambattu Kannan, Solomon F D Paul
OBJECTIVES: Transforming growth factor beta1 (TGF-β1) plays a significant role in craniofacial development. Previous linkage studies reported that the TGF-β1-locus at 19q13.1 harbour predisposing genes for non-syndromic oral clefts. In the present study case parents triads were evaluated to find the transmission effects of genetic variants in TGF- β1 towards non-syndromic cleft lip or palate (NSCL/P). METHODS: Using allelic discrimination method148 families (case-parent triads) were assessed for single nucleotide polymorphisms (SNPs) in TGF-β1 gene...
September 2017: International Journal of Pediatric Otorhinolaryngology
https://www.readbyqxmd.com/read/28802236/tetramethylpyrazine-alleviates-neural-apoptosis-in-injured-spinal-cord-via-the-downregulation-of-mir-214-3p
#4
Yuanzhi Fan, Yaochi Wu
OBJECTIVE: To evaluate the regulation effect of tetramethylpyrazine on microRNA-214-3p (miR-214-3p) in the spinal cord injury (SCI) rats model and to elucidate the neuroprotective effect and its mechanism of tetramethylpyrazine after SCI. METHODS: Ten Sprague-Dawley rats were used to establish the SCI rats model, and the expression levels of miR-214-3p and Bcl2l2 were detected by qRT-PCR and Western blotting at 7 days post-SCI. BBB scoring test was performed to evaluate the motor functional recovery at 21 days post-SCI...
August 9, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28795410/clinicopathological-analysis-of-46-cases-with-cd4-and-or-cd56-immature-hematolymphoid-malignancy-reappraisal-of-blastic-plasmacytoid-dendritic-cell-and-related-neoplasms
#5
Yuka Suzuki, Seiichi Kato, Kei Kohno, Akira Satou, Ahmed E Eladl, Naoko Asano, Michihiro Kono, Yuichi Kato, Masafumi Taniwaki, Masashi Akiyama, Shigeo Nakamura
AIMS: Here we aimed to investigate the clinicopathological features of CD4+ and/or CD56+ immature hematolymphoid malignancy (iHLM), including blastic plasmacytoid dendritic cell neoplasm (BPDCN). METHODS AND RESULTS: We analysed the clinicopathological features of 46 patients consecutively diagnosed with CD4+/CD56+ iHLM. These cases were categorized into three groups based on their immunohistochemical expression of three plasmacytoid dendritic cell (pDC) markers (CD123, CD303, and TCL1): cutaneous BPDCN (n = 35), non-cutaneous BPDCN (n = 6), and non-BPDCN-type CD56+ neoplasms (n = 5)...
August 10, 2017: Histopathology
https://www.readbyqxmd.com/read/28765299/creation-of-recombinant-antibodies-using-5-race-to-amplify-immunoglobulin-sequences
#6
James R Dasch, Amy L Dasch
Modern molecular biology techniques have been applied to the production of therapeutic antibodies. Nonetheless, recombinant antibodies remain the exception rather than the rule for the antibodies used in most research and diagnostic applications. The lack of penetration of recombinant antibodies into the research arena can be attributed largely to the fact that the Ig gene families are large and the variable region gene segments are, indeed, variable, precluding the use of polymerase chain reaction (PCR) with two simple primers to amplify the heavy and light chain gene segments...
August 1, 2017: Cold Spring Harbor Protocols
https://www.readbyqxmd.com/read/28761823/terminal-deoxynucleotidyl-transferase-tdt-inhibiti-on-of-cord-blood-derived-b-and-t-cells-expansion
#7
Sanaz Gholami, Seyede Momeneh Mohammadi, Aliakbar Movasaghpour Akbari, Ali Abedelahi, Alireza Alihemmati, Shirin Fallahi, Hojjatollah Nozad Charoudeh
Purpose: Terminal deoxynucleotidyl transferase(TdT) is a DNA polymerase that is present in immature pre-B and pre-T cells. TdT inserts N-nucleotides to the V (D) J gene segment during rearrangements of genes, therefore, it plays a vital role in the development and variation of the immune system in vertebrates. Here we evaluated the relationship between cytokines like interleukin-2 (IL-2), interleukin-7 (IL-7), and interleukin-15 (IL-15) and TdT expression in cord blood mononuclear cells and also effect of inhibition in the expansion of B and T cells derived from cord blood...
June 2017: Advanced Pharmaceutical Bulletin
https://www.readbyqxmd.com/read/28759343/epidemiologic-study-of-major-complications-in-adolescent-and-adult-patients-with-thalassemia-in-northeastern-thailand-the-e-saan-study-phase-i
#8
Nattiya Teawtrakul, Arunee Jetsrisuparb, Saranya Pongudom, Chittima Sirijerachai, Kanchana Chansung, Chinadol Wanitpongpun, Supan Fucharoen
INTRODUCTION: Thalassemia-related complications are one of the main factors that increase morbidity and mortality in aging patients with thalassemia. This study was aimed to report the prevalence and clinical risk factors for the complications in thalassemia. METHODS: A multi-center prospective cohort study was conducted in patients with thalassemia aged ≥10 years old. Thalassemia-related complications were heart failure, pulmonary hypertension, extramedullary hematopoiesis, endocrine disorders, infections, thrombosis and leg ulcers...
July 31, 2017: Hematology (Amsterdam, Netherlands)
https://www.readbyqxmd.com/read/28753129/glomerular-and-tubular-functions-in-children-and-adults-with-transfusion-dependent-thalassemia
#9
Agageldi Annayev, Zeynep Karakaş, Serap Karaman, Altan Yalçıner, Alev Yılmaz, Sevinç Emre
OBJECTIVE: This study aimed at assessing renal functions in patients with transfusion dependent thalassemia (TDT). MATERIALS AND METHODS: Fifty patients and 30 controls were enrolled in this prospective study. Serum levels of electrolytes and albumin were measured by a spectrophotometer. Serum levels of cystatin-C and urinary levels of β2-microglobulin were measured by nephelometric method. Thirty-eight patients were receiving Deferasirox, 8 were on Deferiprone...
July 28, 2017: Turkish Journal of Haematology: Official Journal of Turkish Society of Haematology
https://www.readbyqxmd.com/read/28745798/research-on-apoptotic-signaling-pathways-of-recurrent-spontaneous-abortion-caused-by-dysfunction-of-trophoblast-infiltration
#10
Q Sun, X-L Zhang
OBJECTIVE: To study the apoptotic signaling pathways of recurrent spontaneous abortion caused by dysfunction of trophoblast infiltration. PATIENTS AND METHODS: 60 patients with recurrent spontaneous abortion and normal abortion were selected consecutively as recurrent spontaneous abortion group and abortion group, respectively. Villous tissues were obtained and cell apoptosis was observed under a microscope; terminal deoxynucleotidyl transferase (TdT)-mediated dUTP nick end labeling (Tunel) method was used to test the apoptosis rate...
July 2017: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/28732374/the-protective-effect-of-pft%C3%AE-on-alcohol-induced-osteonecrosis-of-the-femoral-head
#11
Yi-Xuan Chen, Dao-Yu Zhu, Jun-Hui Yin, Wen-Jing Yin, Yue-Lei Zhang, Hao Ding, Xiao-Wei Yu, Jiong Mei, You-Shui Gao, Chang-Qing Zhang
Epidemiologic studies have shown alcohol plays a pivotal role in the development of osteonecrosis of the femoral head (ONFH). The aim of this study was to explore the underlying mechanism of alcohol-induced ONFH and the protective effect of pifithrin-α (PFTα). In vitro, we found ethanol treatment significantly activated p53, suppressed Wnt/β-catenin signaling and inhibited osteogenic-related proteins. Furthermore, by separating the cytoplasmic and nuclear proteins, we found ethanol inhibited osteogenesis by impairing the accumulation of β-catenin in both the cytoplasm and nucleus in human bone mesenchymal stem cells (hBMSCs), which resulted from activating glycogen synthase kinase-3β (GSK-3β)...
July 11, 2017: Oncotarget
https://www.readbyqxmd.com/read/28729823/administration-of-tauroursodeoxycholic-acid-attenuates-early-brain-injury-via-akt-pathway-activation
#12
Dongdong Sun, Gang Gu, Jianhao Wang, Yan Chai, Yueshan Fan, Mengchen Yang, Xin Xu, Weiwei Gao, Fei Li, Dongpei Yin, Shuai Zhou, Xin Chen, Jianning Zhang
Traumatic brain injury (TBI) is one of the leading causes of trauma-induced mortality and disability, and emerging studies have shown that endoplasmic reticulum (ER) stress plays an important role in the pathophysiology of TBI. Tauroursodeoxycholic acid (TUDCA), a hydrophilic bile acid, has been reported to act as an ER stress inhibitor and chemical chaperone and to have the potential to attenuate apoptosis and inflammation. To study the effects of TUDCA on brain injury, we subjected mice to TBI with a controlled cortical impact (CCI) device...
2017: Frontiers in Cellular Neuroscience
https://www.readbyqxmd.com/read/28722319/immunophenotypic-aberrancies-in-acute-lymphoblastic-leukemia-from-282-iraqi-patients
#13
S D Jalal, N A S Al-Allawi, A A S Al Doski
INTRODUCTION: The identification of aberrancies in leukemia-associated immunophenotype (LAIP) of acute lymphoblastic leukemia (ALL) is quite important in the assessment of minimal residual disease (MRD). This study, the first from Iraq, aimed to assess the frequency and patterns of LAIP among Iraqi patients with ALL, to establish future strategies for evaluating MRD. METHODS: A total of 282 newly diagnosed Iraqi ALL cases were analyzed with six-parameter flow cytometry using a panel of 29 monoclonal antibodies...
July 18, 2017: International Journal of Laboratory Hematology
https://www.readbyqxmd.com/read/28710760/rapid-detection-of-dna-strand-breaks-in-apoptotic-cells-by-flow-and-image-cytometry
#14
Hong Zhao, Zbigniew Darzynkiewicz
Extensive DNA fragmentation that generates a multitude of DNA double-stand breaks (DSBs) is a hallmark of apoptosis. We developed several variants of the widely used TUNEL methodology that is based on the use of exogenous terminal deoxynucleotidyl transferase (TdT) to label 3'OH ends in DSBs with fluorochromes. Flow- or image-cytometry is then employed to detect and quantify apoptotic cells labeled this way. Here, we describe a variant of this technique using BrdUTP as a TdT substrate. The incorporated BrdU is subsequently visualized by a fluorochrome-tagged antibody...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28706437/comparison-of-two-neurotrophic-serpins-reveals-a-small-fragment-with-cell-survival-activity
#15
Paige N Winokur, Preeti Subramanian, Jeanee L Bullock, Veronique Arocas, S Patricia Becerra
PURPOSE: Protease nexin-1 (PN-1), a serpin encoded by the SERPINE2 gene, has serine protease inhibitory activity and neurotrophic properties in the brain. PN-1 inhibits retinal angiogenesis; however, PN-1's neurotrophic capacities in the retina have not yet been evaluated. Pigment epithelium-derived factor (PEDF) is a serpin that exhibits neurotrophic and antiangiogenic activities but lacks protease inhibitory properties. The aim of this study is to compare PN-1 and PEDF. METHODS: Sequence comparisons were performed using computer bioinformatics programs...
2017: Molecular Vision
https://www.readbyqxmd.com/read/28702825/olfactory-dysfunction-and-quality-of-life-in-patients-with-transfusion-dependent-thalassemia
#16
Yasin Yilmaz, Zeynep Karakas, Busra Uzun, Comert Sen, Senol Comoglu, Kadir Serkan Orhan, Selime Aydogdu, Ayse Ozkan Karagenc, Deniz Tugcu, Serap Karaman, Crystal Wylie, Richard L Doty
Transfusion-dependent thalassemia (TDT) is a group of thalassemia syndromes that require regular blood transfusions for survival. It is unknown whether the sense of smell of patients with TDT is affected, and if so, whether smell loss has an adverse effect on quality of life (QOL). Olfactory thresholds were measured using Snap & Sniff(®) wands. QOL was assessed via the Short Form-36 (SF-36) questionnaire. Forty-two TDT patients from the Thalassemia Center in Istanbul Medical Faculty were tested (mean age 26...
July 12, 2017: European Archives of Oto-rhino-laryngology
https://www.readbyqxmd.com/read/28691888/extracellular-signal-regulated-protein-kinases-protein-kinase-b-p53-bh3-interacting-domain-death-agonist-pathway-regulates-gingipain-induced-apoptosis-in-osteoblasts
#17
Fuping Zhang, Qihong Qiu, Xiangchen Song, Yuting Chen, Juan Wu, Min Liang
BACKGROUND: Gingipains are cysteine proteases produced by Porphyromonas gingivalis, the predominant pathogen in chronic periodontitis. The present study aimed to examine the role of gingipains in promoting apoptosis in osteoblasts. METHODS: Human calvarial osteoblasts and osteoblast MC3T3-E1 cells were treated with 8.348 U/L gingipains. Flow cytometry analysis and terminal deoxynucleotidyl transferase (TdT)-mediated deoxy-UTP nick end labeling (TUNEL) staining were used to detect cell apoptosis...
July 10, 2017: Journal of Periodontology
https://www.readbyqxmd.com/read/28690496/dynamic-changes-in-the-renin-angiotensin-aldosterone-system-and-the-beneficial-effects-of-renin-angiotensin-aldosterone-inhibitors-on-spatial-learning-and-memory-in-a-rat-model-of-chronic-cerebral-ischemia
#18
Xinwu Huang, Guozhou Lu, Guochun Li, Hua Li, Beihua Li, Jiazhen Yin, Shousong Cao
Renin-angiotensin-aldosterone system (RAAS) plays an important role in the regulation of blood pressure and brain function. Therefore, we studied the dynamic changes in the RAAS in the blood, cerebral cortex, and hippocampus and the effects of RAAS inhibitors on spatial learning and memory and hippocampal apoptosis in a rat model of chronic cerebral ischemia (CCI) established by bilateral ligation of the common carotid arteries of rats. The levels of renin, angiotensin II (Ang II), and aldosterone (ALD) in the plasma, and the homogenates of the left side of cerebral cortex and whole hippocampus of rats were detected on day 1, 3, 7, 14, 21, and 30 by radioimmunoassay...
2017: Frontiers in Neuroscience
https://www.readbyqxmd.com/read/28688549/curcumin-protects-against-acoustic-trauma-in-the-rat-cochlea
#19
Harun Soyalıç, Fikret Gevrek, Serhat Karaman
OBJECTIVES: In this study we evaluated the therapeutic utility of curcumin in a rodent model of acoustic trauma using histopathology, immunohistochemical, and distortion product otoacoustic emission (DPOAEs) measurements. METHODS: 28 Wistar albino rats were included in the study and randomly assigned to 4 treatment groups. The first group (group 1) served as the control and was exposed to acoustic trauma alone. Group 2 was the curcumin group. Group 3 was the curcumin plus acoustic trauma group...
August 2017: International Journal of Pediatric Otorhinolaryngology
https://www.readbyqxmd.com/read/28681127/selenium-antagonizes-the-lead-induced-apoptosis-of-chicken-splenic-lymphocytes-in-vitro-by-activating-the-pi3k-akt-pathway
#20
Da Zhao, Xinyan Zhang
Lead (Pb) pollution has become one of the most serious global ecological problems. In animals, Pb ingestion induces apoptosis in many tissues. However, the mechanisms by which Pb induces apoptosis in chicken splenic lymphocytes in vitro via the PI3K/Akt pathway and the antagonistic effect of selenium (Se) on Pb remain unclear. Therefore, we established the in vitro Se-Pb interaction model in chicken splenic lymphocytes and examined the frequency of apoptotic cells using acridine orange/ethidium bromide (AO/EB) staining and the TdT-mediated dUTP nick end labeling assay and detected the activities of glutathione peroxidase (GPx), superoxide dismutase (SOD), and catalase (CAT), as well as the levels of malondialdehyde (MDA) and reactive oxygen species (ROS)...
July 5, 2017: Biological Trace Element Research
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