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https://www.readbyqxmd.com/read/28823125/the-importance-of-post-thaw-subculture-for-standardizing-cellular-activity-of-fresh-or-cryopreserved-mouse-embryonic-stem-cells
#1
Dong Woo Ko, Jung Ki Yoon, Jong Il Ahn, Myungook Lee, Woo Sub Yang, Ji Yeon Ahn, Jeong Mook Lim
Objective: Much difference in cellular activity was detected between early and late subpassaged embryonic stem cell (ESCs) lines, which can be created by subtle changes in cell manipulation protocol. This study was subsequently examined whether post-thaw subculture of early subpassaged ESC lines could further affect their cellular activity, which results can contribute to standardizing ESC manipulation protocol for minimizing batch difference or data fluctuation. Methods: Fresh (as a control treatment) or cryopreserved F1 hybrid (B6CBAF1) early ESC lines (C57BL/6xCBA) of the 4 (P4) or the 19 passage (P19) were subcultured once, twice or six times under the same condition...
August 16, 2017: Asian-Australasian Journal of Animal Sciences
https://www.readbyqxmd.com/read/28823119/determinants-of-health-related-quality-of-life-in-iranian-adults-evidence-from-a-cross-sectional-study
#2
Satar Rezaei, Mohammad Hajizadeh, Ali Kazemi, Masoud Khosravipour, Farid Khosravi, Shahab Rezaeian
Purpose: This study aimed to measure the level and determinants of health-related quality of life (HRQoL) in adults in Kermanshah province, the western region of Iran. Methods: Convenience sampling was employed to derive a sample of 998 adults 18 years and older (646 males and 352 females) in Kermanshah city. A two-part self-administered questionnaire was used for data collection. The first part designed to collect information on socio-demographic characteristics, socioeconomic status and life style factors...
August 15, 2017: Epidemiology and Health
https://www.readbyqxmd.com/read/28823111/welfare-to-work-interventions-and-their-effects-on-the-mental-and-physical-health-of-lone-parents-and-their-children
#3
REVIEW
Marcia Gibson, Hilary Thomson, Kasia Banas, Vittoria Lutje, Martin J McKee, Susan P Martin, Candida Fenton, Clare Bambra, Lyndal Bond
BACKGROUND: Lone parents in high-income countries have high rates of poverty (including in-work poverty) and poor health. Employment requirements for these parents are increasingly common. 'Welfare-to-work' (WtW) interventions involving financial sanctions and incentives, training, childcare subsidies and lifetime limits on benefit receipt have been used to support or mandate employment among lone parents. These and other interventions that affect employment and income may also affect people's health, and it is important to understand the available evidence on these effects in lone parents...
August 20, 2017: Cochrane Database of Systematic Reviews
https://www.readbyqxmd.com/read/28823084/effects-of-a-novel-tungsten-impregnated-rubber-neck-shield-on-the-quality-of-cerebral-images-acquired-using-15-o-labeled-gas
#4
Kei Wagatsuma, Keiichi Oda, Kenta Miwa, Motoki Inaji, Muneyuki Sakata, Jun Toyohara, Kiichi Ishiwata, Masayuki Sasaki, Kenji Ishii
The present study aimed to validate the effects of a novel tungsten-impregnated rubber neck shield on the quality of phantom and clinical (15)O-labeled gas positron emission tomography (PET) images. Images were acquired in the presence or absence of a neck shield from a cylindrical phantom containing [(15)O]H2O (phantom study) and from three individuals using [(15)O]CO2, [(15)O]O2 and [(15)O]CO gas (clinical study). Data were acquired in three-dimensional (3D) mode using a Discovery PET/CT 710. Values for cerebral blood flow, cerebral blood volume, oxygen extraction fraction, and cerebral metabolic rate of oxygen with and without the neck shield were calculated from (15)O-labeled gas images...
August 19, 2017: Radiological Physics and Technology
https://www.readbyqxmd.com/read/28823022/long-term-bisphosphonate-therapy-in-osteogenesis-imperfecta
#5
REVIEW
A Biggin, C F Munns
PURPOSE OF REVIEW: Osteogenesis imperfecta (OI) is a genetic bone disorder resulting in bone fragility. It has a heterogeneous phenotype which typically includes reduced bone mass, multiple fractures, deformity, and chronic disability. Bisphosphonate treatment remains the first-line medical management, but there is still debate on aspects of its effectiveness. This review summarizes current knowledge about long-term bisphosphonate use in OI with recommendations on clinical application...
August 19, 2017: Current Osteoporosis Reports
https://www.readbyqxmd.com/read/28823014/assessment-of-the-quality-of-the-harran-plain-soils-under-long-term-cultivation
#6
Ali Volkan Bilgili, Çiğdem Küçük, Harold M Van Es
Soil quality refers to the ability of soils to perform their functions well. The soils of the Harran Plain, Turkey, have been put into intensive crop production with the introduction of an irrigation scheme and become increasingly degraded due to unsustainable management and cropping systems that resulted in the loss of production potential. The goal of this study was to quantify the quality of common soil series in the Plain using soil quality indexes (SQI) and to compare SQIs of two long-term crop rotations, cotton and wheat-corn cultivation, and different soil types...
August 19, 2017: Environmental Monitoring and Assessment
https://www.readbyqxmd.com/read/28822961/use-of-key-performance-indicators-in-histological-dissection
#7
Matthew Griffiths, Rachel Gillibrand
AIMS: Reports into standards in the National Health Service and quality in pathology have focused on the way we work in pathology and how to provide assurance that this is of a high standard. There are a number of external quality assurance schemes covering pathology and histopathology specifically; however, there is no scheme covering the process of histological surgical dissection. This is an area undergoing development, emerging from the sole preserve of medically qualified pathologists to a field populated by a number of highly trained biomedical scientists, but remains without any formal quality assurance...
August 19, 2017: Journal of Clinical Pathology
https://www.readbyqxmd.com/read/28822922/prevalence-and-socio-demographic-characteristics-of-disability-in-older-adults-in-china-findings-from-china-comprehensive-geriatric-assessment-study
#8
Lina Ma, Zhenzhen Li, Zhe Tang, Fei Sun, Lijun Diao, Jian Li, Yao He, Birong Dong, Yun Li
OBJECTIVE: Disability affects older adults' quality of life. This study aimed to examine the socio-demographic characteristics of disability in older adults in China. METHOD: Data was obtained from the China Comprehensive Geriatric Assessment Study (CCGAS). The sample comprised 6864 people aged 60 years and above from seven provinces in China. A door-to-door survey was conducted by formally trained interviewers using a unified questionnaire. Disability was assessed with physical health assessment comprising activities of daily living (ADL), and independent activities of daily living (IADL)...
August 3, 2017: Archives of Gerontology and Geriatrics
https://www.readbyqxmd.com/read/28822904/the-effect-of-age-and-length-of-gonadotropin-stimulation-on-the-in%C3%A2-vitro-embryo-development-of-holstein-calf-oocytes
#9
Luke Currin, Laura Michalovic, Anne-Marie Bellefleur, Karina Gutierrez, Werner Glanzner, Yasmin Schuermann, Rodrigo C Bohrer, Naomi Dicks, Paulo R da Rosa, Matheus P De Cesaro, Rosalba Lopez, François-Xavier Grand, Christian Vigneault, Patrick Blondin, Jim Gourdon, Hernan Baldassarre, Vilceu Bordignon
The use of oocytes recovered from prepubertal donors for in vitro embryo production has great potential for accelerating the rate of genetic gain in the dairy industry. However, these oocytes are known to be less developmentally competent than those from adult donors. In this study, we investigated the effect of age and gonadotropin stimulation in Holstein heifers subjected to oocyte collection every two weeks between 2 and 6 months of age. In order to assess the effect of gonadotropin stimulation, animals were subjected to one of three treatments, namely Short (ST; 36-42 h), Long (LT; ≥72 h) and No Treatment (NT) prior to laparoscopic ovum pick-up (LOPU)...
August 10, 2017: Theriogenology
https://www.readbyqxmd.com/read/28822900/supportive-care-pathway-functionalities-of-ehr-system-in-a-saudi-arabian-hospital
#10
Bakheet Aldosari
BACKGROUND: Electronic health record (EHR) is a data management system used in healthcare services. The stored medical information in EHR can be accessed and utilized by authorized users, which helps clinicians in making clinical decisions. Therefore, this study aims at comparing the embedded clinical guidelines or the supportive pathway functions as well as their compliance level in the two EMR systems, i.e. QuadraMed (QCPR) and BestCare. METHODS: A test vignette was used to evaluate the features, functions, performance, and output to assess the EHR clinical path way functionality of the QCPR and BestCare...
August 12, 2017: Computers in Biology and Medicine
https://www.readbyqxmd.com/read/28822841/the-effciency-and-safety-of-tvt-abbrevo-versus-tvt-exact-in-the-normal-weight-and-overweight-patients-affected-by-stress-urinary-incontinence
#11
Yi Sun, Deyi Luo, Lu Yang, Xin Wei, Cai Tang, Mei Chen, Hong Shen, Qiang Wei
OBJECTIVE: To compare the compare efficacy between the two differernt slings in normal weight and overweight women. METHODS: There were 426 women, 220 women (119 normal weight and 101 overweight) accepted the TVT-A and 206 womens (141 normal weight and 92 overweight) accepted the TVT-E procedure. Data collected contained the subjectively efficiency, objectively efficiency, Questionnaire-Short Form (ICIQ-SF), Incontinence Quality Of Life questionnaire (I-QOL), Pelvic Floor Impact Questionnaire short form (PFIQ-7), Urogenital Distress Inventory short form (UDI-6) and (Pelvic Organ Prolapse/Urinary Incontinence Sexual Questionnaire short form) PISQ-12 at the 36 months after the surgery...
August 16, 2017: Urology
https://www.readbyqxmd.com/read/28822795/strategies-for-analyzing-bisulfite-sequencing-data
#12
Katarzyna Wreczycka, Alexander Gosdschan, Dilmurat Yusuf, Björn Grüning, Yassen Assenov, Altuna Akalin
DNA methylation is one of the main epigenetic modifications in the eukaryotic genome; it has been shown to play a role in cell-type specific regulation of gene expression, and therefore cell-type identity. Bisulfite sequencing is the gold-standard for measuring methylation over the genomes of interest. Here, we review several techniques used for the analysis of high-throughput bisulfite sequencing. We introduce specialized short-read alignment techniques as well as pre/post-alignment quality check methods to ensure data quality...
August 16, 2017: Journal of Biotechnology
https://www.readbyqxmd.com/read/28822778/diabetes-care-program-of-nova-scotia-celebrating-25-years-of-improving-diabetes-care-in-nova-scotia
#13
REVIEW
Jennifer I Payne, Margaret J Dunbar, Pamela Talbot, Meng H Tan
The Diabetes Care Program of Nova Scotia (DCPNS)'s mission is "to improve, through leadership and partnerships, the health of Nova Scotians living with, affected by, or at risk of developing diabetes." Working together with local, provincial and national partners, the DCPNS has improved and standardized diabetes care in Nova Scotia over the past 25 years by developing and deploying a resourceful and collaborative program model. This article describes the model and highlights its key achievements. With balanced representation from frontline providers through to senior decision makers in health care, the DCPNS works across the age continuum, supporting the implementation of national clinical practice guidelines and, when necessary, developing provincial guidelines to meet local needs...
August 16, 2017: Canadian Journal of Diabetes
https://www.readbyqxmd.com/read/28822682/a-systematic-review-of-digital-interventions-for-improving-the-diet-and-physical-activity-behaviors-of-adolescents
#14
REVIEW
Taylor Rose, Mary Barker, Chandni Maria Jacob, Leanne Morrison, Wendy Lawrence, Sofia Strömmer, Christina Vogel, Kathryn Woods-Townsend, David Farrell, Hazel Inskip, Janis Baird
Many adolescents have poor diet and physical activity behaviors, which can lead to the development of noncommunicable diseases in later life. Digital platforms offer inexpensive means of delivering health interventions, but little is known about their effectiveness. This systematic review was conducted to synthesize evidence on the effectiveness of digital interventions to improve diet quality and increase physical activity in adolescents, to effective intervention components and to assess the cost-effectiveness of these interventions...
August 16, 2017: Journal of Adolescent Health: Official Publication of the Society for Adolescent Medicine
https://www.readbyqxmd.com/read/28822680/outcomes-of-endovascular-abdominal-aortic-aneurysm-repair-in-octogenarians-meta-analysis-and-systemic-review
#15
REVIEW
Y Han, S Zhang, J Zhang, C Ji, H-H Eckstein
OBJECTIVES: This meta-analysis and systematic review aimed to highlight the results of endovascular aneurysm repair (EVAR) for abdominal aortic aneurysm (AAA) to assess safety and effectiveness in patients older than 80 years of age compared with younger patients. METHODS: PRISMA guidelines were used; quality was appraised, and data were extracted and analysed following the Cochrane collaboration. The MEDLINE and Embase databases were searched on December 31, 2016...
August 16, 2017: European Journal of Vascular and Endovascular Surgery
https://www.readbyqxmd.com/read/28822657/a-systematic-review-of-muscle-morphology-and-function-in-intermittent-claudication
#16
REVIEW
Amy E Harwood, Stephanie King, Joshua Totty, George E Smith, Natalie Vanicek, Ian C Chetter
OBJECTIVE: Intermittent claudication (IC) is frequently associated with deterioration in walking capacity and physical function, and it can often result in an impairment in balance. Whereas supervised exercise is recommended by the National Institute for Health and Care Excellence as the first-line treatment, the mechanism behind walking improvement is poorly understood. The existing literature suggests that there may be some physiologic change to the skeletal muscle contributing to the functional impairment, but these data are conflicting...
August 16, 2017: Journal of Vascular Surgery
https://www.readbyqxmd.com/read/28822656/phenylephrine-infusion-impact-on-surgical-site-infections-after-lower-extremity-bypass-surgery
#17
Craig Curry, Jens Eldrup-Jorgensen, Janelle Richard, Michele C Siciliano, Wendy Y Craig
OBJECTIVE: Lower extremity bypass (LEB) operations have high rates of surgical site infections (SSI). Phenylephrine is a commonly used vasoconstrictor which may reduce skin blood flow and increase the likelihood of SSI in these patients. We studied the potential effect of phenylephrine infusion during LEB surgery on SSI. METHODS: LEB cases and their demographic data were identified through the Vascular Quality Initiative registry. SSI in this population was identified using the hospital epidemiology surveillance database...
August 16, 2017: Journal of Vascular Surgery
https://www.readbyqxmd.com/read/28822644/extracting-general-practice-data-for-timely-vaccine-coverage-estimates-the-england-experience
#18
Michael Edelstein, Tim Crocker-Buque, Camille Tsang, Odette Eugenio, Tim Hopson, Richard Pebody, Mary Ramsay, Joanne M White
In England, primary care providers use standardised coding systems to record health events such as vaccination as well as patient characteristics. This information can be automatically extracted to estimate coverage for vaccine programmes delivered through primary care, in the general population as well as in specific geographical, ethnic, age or clinical groups. This system provides timely vaccine coverage estimates as well as the flexibility to extract tailored data in order to directly inform a continuously evolving national vaccine programme...
August 16, 2017: Vaccine
https://www.readbyqxmd.com/read/28822600/factors-associated-with-physical-activity-participation-in-adults-with-chronic-cervical-spine-pain-a-systematic-review
#19
REVIEW
Michael Mansfield, Michael Thacker, Nicolas Spahr, Toby Smith
OBJECTIVE: To determine the factors associated with physical activity participation in adults with chronic cervical spine pain. METHODS: A systematic review was conducted including searches of PubMed (MEDLINE), EMBASE and CINAHL from inception to June 12th, 2016. Grey literature and reference checking was also undertaken. Quantitative studies including factors related to physical activity participation in adults with chronic cervical spine pain were included. Two independent authors conducted the searches, extracted data and completed methodological quality assessment...
February 13, 2017: Physiotherapy
https://www.readbyqxmd.com/read/28822527/coronary-artery-disease-in-women
#20
REVIEW
Lekha Adik Pathak, Salil Shirodkar, Ronak Ruparelia, Jaideep Rajebahadur
BACKGROUND: Despite the importance of CAD for women, there is persistent perception that CAD is a man's disease. Contributing to this notion is the observation of differences in incidence rates according to age; the incidence of CAD in women is lower than men, but rises steadily after fifth decade. The distribution of CAD risk factors varies between men and women across age ranges and failure to consider these differences may have contributed to the belief that women are at lower risk of CAD compared with men...
July 2017: Indian Heart Journal
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