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https://www.readbyqxmd.com/read/28805368/pyrophosphate-recognition-and-sensing-in-water-using-bis-zinc-ii-dipicolylamino-functionalized-peptides
#1
Katrina A Jolliffe
Phosphate oxoanions and phosphorylated biomolecules (such as nucleotides, lipids, and proteins) play key roles in a wide range of biological processes. The ability to selectively detect these ions in the presence of each other has numerous applications in biochemistry and biomedicine. However, receptors and sensors that can discriminate between polyphosphate species with high selectivity and in biologically relevant conditions are rare. In this Account, we show how peptides (both cyclic and linear) can be used to position two zinc(II)dipicolylamine [Zn(II)DPA] binding sites in an appropriate arrangement to provide selective binding of pyrophosphate (PPi) in the presence of other polyphosphate species, including ATP, and in complex media such as cell growth buffer...
August 14, 2017: Accounts of Chemical Research
https://www.readbyqxmd.com/read/28804996/in-vitro-evaluation-of-3d-bioprinted-tri-polymer-network-scaffolds-for-bone-tissue-regeneration
#2
Stephanie T Bendtsen, Mei Wei
In vitro evaluations provide vital information on the ability of tissue engineered scaffolds to support cell life and promote natural physiological behaviors in culture. Such assessments are necessary to conduct before implementation of the scaffolds for tissue healing in vivo. The scaffold extracellular matrix must provide the biochemical and mechanical cues necessary to promote cellular attachment, migration and proliferation before differentiation and new tissue deposition can occur. In this study, an in vitro evaluation was conducted to assess the ability of scaffolds 3D printed with a previously developed alginate-polyvinyl alcohol-hydroxyapatite formulation to promote proliferation of encapsulated MC3T3 cells...
August 14, 2017: Journal of Biomedical Materials Research. Part A
https://www.readbyqxmd.com/read/28803478/biomimetic-bone-like-hydroxyapatite-by-mineralization-on-supramolecular-porous-fiber-networks
#3
Bo Li, Lei Kan, Xinyue Zhang, Jie Li, Ruiting Li, Qinyuan Gui, Dengli Qiu, Fei He, Ning Ma, Yapei Wang, Hao Wei
The hydroxyapatite (HA), main inorganic component of bone tissue, is mineralized with collagen fibril scaffolds during bone formation. Inspired by the process, self-assembled porous networks architecture was designed and synthesized by using the 2-ureido-4[1H]-pyrimidone (UPy) modified glycerol molecule UPy-Gly, which was further utilized as a template for biomimetic mineralization. When incubated in simulated body fluid (SBF), the HA nucleus firstly formed in the holes of the template by the induction of hydroxyls on the surface, grew along the nanofibers and fused with the template to fabricate the hydroxyapatite composites (UPy-Gly/HA)...
August 13, 2017: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/28801269/elastin-plga-hybrid-electrospun-nanofiber-scaffolds-for-salivary-epithelial-cell-self-organization-and-polarization
#4
Zahraa I Foraida, Tim Kamaldinov, Deirdre A Nelson, Melinda Larsen, James Castracane
Development of electrospun nanofibers that mimic the structural, mechanical and biochemical properties of natural extracellular matrices (ECMs) is a promising approach for tissue regeneration. Electrospun fibers of synthetic polymers partially mimic the topography of the ECM, however, their high stiffness, poor hydrophilicity and lack of in vivo-like biochemical cues is not optimal for epithelial cell self-organization and function. In search of a biomimetic scaffold for salivary gland tissue regeneration, we investigated the potential of elastin, an ECM protein, to generate elastin hybrid nanofibers that have favorable physical and biochemical properties for regeneration of the salivary glands...
August 8, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/28800071/fabrication-and-characterization-of-magnesium-ferrite-based-pcl-aloe-vera-nanofibers
#5
Zanshe Thompson, Shekh Rahman, Sergey Yarmolenko, Jagannathan Sankar, Dhananjay Kumar, Narayan Bhattarai
Composite nanofibers of biopolymers and inorganic materials have been widely explored as tissue engineering scaffolds because of their superior structural, mechanical and biological properties. In this study, magnesium ferrite (Mg-ferrite) based composite nanofibers were synthesized using an electrospinning technique. Mg-ferrite nanoparticles were first synthesized using the reverse micelle method, and then blended in a mixture of polycaprolactone (PCL), a synthetic polymer, and Aloe vera, a natural polymer, to create magnetic nanofibers by electrospinning...
August 11, 2017: Materials
https://www.readbyqxmd.com/read/28799168/small-scaffolds-big-potential-developing-miniature-proteins-as-therapeutic-agents
#6
Justin M Holub
Preclinical Research Miniature proteins are a class of oligopeptide characterized by their short sequence lengths and ability to adopt well-folded, three-dimensional structures. Because of their biomimetic nature and synthetic tractability, miniature proteins have been used to study a range of biochemical processes including fast protein folding, signal transduction, catalysis and molecular transport. Recently, miniature proteins have been gaining traction as potential therapeutic agents because their small size and ability to fold into defined tertiary structures facilitates their development as protein-based drugs...
August 10, 2017: Drug Development Research
https://www.readbyqxmd.com/read/28798993/decellularization-and-recellularization-of-cartilage
#7
Catherine A Bautista, Bahar Bilgen
Decellularization of cartilage enables the use of cartilage allografts or xenografts as natural scaffolds for repair and regeneration of injured cartilage. The preservation of the extracellular matrix ultrastructure of the graft makes this a promising tool for cartilage tissue engineering. We have optimized the decellularization protocol by enzymatically digesting proteoglycans while preserving the native collagen architecture. Here we describe our methods for cartilage decellularization and cell labeling for the tracking of infiltration for recellularization in detail...
August 11, 2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28798872/fiber-reinforced-scaffolds-in-soft-tissue-engineering
#8
REVIEW
Baoqing Pei, Wei Wang, Yubo Fan, Xiumei Wang, Fumio Watari, Xiaoming Li
Soft tissue engineering has been developed as a new strategy for repairing damaged or diseased soft tissues and organs to overcome the limitations of current therapies. Since most of soft tissues in the human body are usually supported by collagen fibers to form a three-dimensional microstructure, fiber-reinforced scaffolds have the advantage to mimic the structure, mechanical and biological environment of natural soft tissues, which benefits for their regeneration and remodeling. This article reviews and discusses the latest research advances on design and manufacture of novel fiber-reinforced scaffolds for soft tissue repair and how fiber addition affects their structural characteristics, mechanical strength and biological activities in vitro and in vivo...
August 2017: Regenerative Biomaterials
https://www.readbyqxmd.com/read/28796191/smart-carriers-and-nanohealers-a-nanomedical-insight-on-natural-polymers
#9
REVIEW
Sreejith Raveendran, Ankit K Rochani, Toru Maekawa, D Sakthi Kumar
Biodegradable polymers are popularly being used in an increasing number of fields in the past few decades. The popularity and favorability of these materials are due to their remarkable properties, enabling a wide range of applications and market requirements to be met. Polymer biodegradable systems are a promising arena of research for targeted and site-specific controlled drug delivery, for developing artificial limbs, 3D porous scaffolds for cellular regeneration or tissue engineering and biosensing applications...
August 10, 2017: Materials
https://www.readbyqxmd.com/read/28794849/scaffolds-for-retinal-pigment-epithelial-cell-transplantation-in-age-related-macular-degeneration
#10
REVIEW
Corina E White, Ronke M Olabisi
In several retinal degenerative diseases, including age-related macular degeneration, the retinal pigment epithelium, a highly functionalized cell monolayer, becomes dysfunctional. These retinal diseases are marked by early retinal pigment epithelium dysfunction reducing its ability to maintain a healthy retina, hence making the retinal pigment epithelium an attractive target for treatment. Cell therapies, including bolus cell injections, have been investigated with mixed results. Since bolus cell injection does not promote the proper monolayer architecture, scaffolds seeded with retinal pigment epithelium cells and then implanted have been increasingly investigated...
January 2017: Journal of Tissue Engineering
https://www.readbyqxmd.com/read/28794415/living-genochemetics-by-hyphenating-synthetic-biology-and-synthetic-chemistry-in-vivo
#11
Sunil V Sharma, Xiaoxue Tong, Cristina Pubill-Ulldemolins, Christopher Cartmell, Emma J A Bogosyan, Emma J Rackham, Enrico Marelli, Refaat B Hamed, Rebecca J M Goss
Marrying synthetic biology with synthetic chemistry provides a powerful approach toward natural product diversification, combining the best of both worlds: expediency and synthetic capability of biogenic pathways and chemical diversity enabled by organic synthesis. Biosynthetic pathway engineering can be employed to insert a chemically orthogonal tag into a complex natural scaffold affording the possibility of site-selective modification without employing protecting group strategies. Here we show that, by installing a sufficiently reactive handle (e...
August 9, 2017: Nature Communications
https://www.readbyqxmd.com/read/28792478/marine-inspired-2-5-halo-1h-indol-3-yl-n-n-dimethylethanamines-as-modulators-of-serotonin-receptors-an-example-illustrating-the-power-of-bromine-as-part-of-the-uniquely-marine-chemical-space
#12
Mohamed A Ibrahim, Abir T El-Alfy, Kelly Ezel, Mohamed O Radwan, Abbas G Shilabin, Anna J Kochanowska-Karamyan, Howaida I Abd-Alla, Masami Otsuka, Mark T Hamann
In previous studies, we have isolated several marine indole alkaloids and evaluated them in the forced swim test (FST) and locomotor activity test, revealing their potential as antidepressant and sedative drug leads. Amongst the reported metabolites to display such activities was 5-bromo-N,N-dimethyltryptamine. Owing to the importance of the judicious introduction of halogens into drug candidates, we synthesized two series built on a 2-(1H-indol-3-yl)-N,N-dimethylethanamine scaffold with different halogen substitutions...
August 9, 2017: Marine Drugs
https://www.readbyqxmd.com/read/28782480/recent-progress-in-biological-activities-of-indole-and-indole-alkaloids
#13
O M Singh, Thokchom Prasanta Singh
The indole scaffold is a prominent and privileged structural motif found in numerous natural products and in a wide range of bioactive heterocycles. Starting from the simple structural framework of serotonin, the well known neurotransmitter to complex alkaloids which are used clinically as anticancer agent, mitomycin C, and the antihypertensive alkaloid reserpine, the indole moiety is considered as the active principle. Thus over the past decade, a large number of indole-containing compounds have revealed remarkable pharmacological activity and their utility as therapeutic agents have attracted considerable attention from chemists...
August 7, 2017: Mini Reviews in Medicinal Chemistry
https://www.readbyqxmd.com/read/28779844/saposin-lipoprotein-scaffolds-for-structure-determination-of-membrane-transporters
#14
Joseph A Lyons, Andreas Bøggild, Poul Nissen, Jens Frauenfeld
Membrane proteins depend on their natural lipid environment for function, which makes them more difficult to study in isolation. A number of approaches that mimic the lipid bilayer of biological membranes have been described (nanodiscs, SMALPs), enabling novel ways to assay activity and elucidate structures of this important class of proteins. More recently, the use of saposin A, a protein that is involved in lipid transport, to form Salipro (saposin-lipid-protein) complexes was demonstrated for a range of membrane protein targets (Frauenfeld et al...
2017: Methods in Enzymology
https://www.readbyqxmd.com/read/28777137/natural-compounds-for-skin-tissue-engineering-by-electrospinning-of-nylon-beta-vulgaris
#15
Parviz Ranjbarvan, Matin Mahmoudifard, Mousa Kehtari, Ali Babaie, Shokoh Hamedi, Samaneh Mirzaei, Masoud Soleimani, Simzar Hosseinzadeh
Natural compounds containing polysaccharide ingredients have been employed as candidates for treatment of skin tissue. Herein, for the first time, electrospinning setup was proposed to fabricate an efficient composite nanofibrous structure of Beta vulgaris (obtained from Beet [Chenopodiaceae or Amaranthaceae]) belonged to polysaccharides and an elastic polymer named nylon 66 for skin tissue engineering. Both prepared scaffolds including noncomposite and composite types were studied by Scanning electron microscope (SEM), FTIR spectroscopy, mechanical assay, and contact angle...
August 2, 2017: ASAIO Journal: a Peer-reviewed Journal of the American Society for Artificial Internal Organs
https://www.readbyqxmd.com/read/28776178/protocols-for-rat-uterus-isolation-and-decellularization-applications-for-uterus-tissue-engineering-and-3d-cell-culturing
#16
Arvind Manikantan Padma, Tom Tristan Tiemann, Ahmed Baker Alshaikh, Randa Akouri, Min Jong Song, Mats Hellström
Sophisticated culturing conditions are required to grow cells in a three-dimensional (3D) environment. Cells then require a type of scaffold rich in proteins, growth factors, and signaling molecules that simulates their natural environment. Tissues from all species of animals have an organ-specific extracellular matrix (ECM) structure that plays a key role in cell proliferation and migration. Hence, the scaffold composition plays a significant role for any successful 3D cell culturing system. We developed a whole rat uterus ECM scaffold by the perfusion of detergents and ionic solutions through the vascular system of an isolated normal rat uterus in a process termed "decellularization...
August 4, 2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28774427/synthesis-of-antimalarial-amide-analogues-based-on-the-plant-serrulatane-diterpenoid-3-7-8-trihydroxyserrulat-14-en-19-oic-acid
#17
Rohitesh Kumar, Sandra Duffy, Vicky M Avery, Rohan A Davis
A plant-derived natural product scaffold, 3,7,8-trihydroxyserrulat-14-en-19-oic acid (1) was isolated in high yield from the aerial parts of the endemic Australian desert plant Eremophila microtheca. This scaffold (1) was subsequently used in the generation of a series of new amide analogues via a one-pot mixed anhydride amidation using pivaloyl chloride. The structures of all analogues were characterized using MS, NMR, and UV data. The major serrulatane natural products (1-3), isolated from the plant extract, and all amide analogues (6-15) together with several pivaloylated derivatives of 3,7,8-trihydroxyserrulat-14-en-19-oic acid (16-18) were evaluated for their antimalarial activity against 3D7 (chloroquine sensitive) and Dd2 (chloroquine resistant) Plasmodium falciparum strains, and preliminary cytotoxicity data were also acquired using the human embryonic kidney cell line HEK293...
July 15, 2017: Bioorganic & Medicinal Chemistry Letters
https://www.readbyqxmd.com/read/28772664/xylan-modified-based-hydrogels-with-temperature-ph-dual-sensitivity-and-controllable-drug-delivery-behavior
#18
Wei-Qing Kong, Cun-Dian Gao, Shu-Feng Hu, Jun-Li Ren, Li-Hong Zhao, Run-Cang Sun
Among the natural macromolecules potentially used as the scaffold material in hydrogels, xylan has aroused great interest in many fields because of its biocompatibility, low toxicity, and biodegradability. In this work, new pH and thermoresponsive hydrogels were prepared by the cross-linking polymerization of maleic anhydride-modified xylan (MAHX) with N-isopropylacrylamide (NIPAm) and acrylic acid (AA) under UV irradiation to form MAHX-g-P(NIPAm-co-AA) hydrogels. The pore volume, the mechanical properties, and the release rate for drugs of hydrogels could be controlled by the degree of substitution of MAHX...
March 16, 2017: Materials
https://www.readbyqxmd.com/read/28772530/coaxial-mos%C3%A2-carbon-hybrid-fibers-a-low-cost-anode-material-for-high-performance-li-ion-batteries
#19
Rui Zhou, Jian-Gan Wang, Hongzhen Liu, Huanyan Liu, Dandan Jin, Xingrui Liu, Chao Shen, Keyu Xie, Bingqing Wei
A low-cost bio-mass-derived carbon substrate has been employed to synthesize MoS₂@carbon composites through a hydrothermal method. Carbon fibers derived from natural cotton provide a three-dimensional and open framework for the uniform growth of MoS₂ nanosheets, thus hierarchically constructing coaxial architecture. The unique structure could synergistically benefit fast Li-ion and electron transport from the conductive carbon scaffold and porous MoS₂ nanostructures. As a result, the MoS₂@carbon composites-when serving as anodes for Li-ion batteries-exhibit a high reversible specific capacity of 820 mAh·g(-1), high-rate capability (457 mAh·g(-1) at 2 A·g(-1)), and excellent cycling stability...
February 13, 2017: Materials
https://www.readbyqxmd.com/read/28771114/beneficial-effect-of-aligned-nanofiber-scaffolds-with-electrical-conductivity-for-the-directional-guide-of-cells
#20
Bon Kang Gu, Sang Jun Park, Chun-Ho Kim
Conducting polymer-based scaffolds receive biological and electrical signals from the extracellular matrix (ECM) or peripheral cells, thereby promoting cell growth and differentiation. Chitin, a natural polymer, is widely used as a scaffold because it is biocompatible, biodegradable, and nontoxic. In this study, we used an electrospinning technique to fabricate conductive scaffolds from aligned chitin/polyaniline (Chi/PANi) nanofibers for the directional guidance of cells. Pure chitin and random and aligned Chi/PANi nanofiber scaffolds were characterized using field emission scanning electron microscope (FE-SEM) and by assessing wettability, mechanical properties, and electrical conductivity...
August 3, 2017: Journal of Biomaterials Science. Polymer Edition
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