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https://www.readbyqxmd.com/read/29150791/16-channel-flexible-system-to-measure-electrophysiological-properties-of-bioengineered-hearts
#1
Betsy H Salazar, Kristopher A Hoffman, Anilkumar K Reddy, Sridhar Madala, Ravi K Birla
As tissue engineering continues to mature, it is necessary to develop new technologies that bring insight into current paradigms and guide improvements for future experiments. To this end, we have developed a system to characterize our bioartificial heart model and compare them to functional native structures. In the present study, the hearts of adult Sprague-Dawley were decellularized resulting in a natural three-dimensional cardiac scaffold. Neonatal rat primary cardiac cells were then cultured within a complex 3D fibrin gel, forming a 3-dimensional cardiac construct, which was sutured to the acellular scaffold and suspended in media for 24-48 h...
November 17, 2017: Cardiovascular Engineering and Technology
https://www.readbyqxmd.com/read/29148982/a-dentin-derived-hydrogel-bioink-for-3d-printing-of-cell-laden-scaffolds-for-regenerative-dentistry
#2
Avathamsa Athirasala, Anthony Tahayeri, Greeshma Thrivikraman, Cristiane Miranda Franca, Nelson Monteiro, Victor Tran, Jack Ferracane, Luiz Bertassoni
Recent studies in tissue engineering have adopted extracellular matrix (ECM) derived scaffolds as natural and cytocompatible microenvironments for tissue regeneration. The dentin matrix, specifically, has been shown to be associated with a host of soluble and insoluble signaling molecules that can promote odontogenesis. Here, we have developed a novel bioink, blending printable alginate (3% w/v) hydrogels with the soluble and insoluble fractions of the dentin matrix. We have optimized the printing parameters and the concentrations of the individual components of the bioink for print accuracy, cell viability and odontogenic potential...
November 17, 2017: Biofabrication
https://www.readbyqxmd.com/read/29148767/role-of-the-benzodioxole-group-in-the-interactions-between-the-natural-alkaloids-chelerythrine-and-coptisine-and-the-human-telomeric-g-quadruplex-dna-a-multiapproach-investigation
#3
F Papi, M Ferraroni, R Rigo, S Da Ros, C Bazzicalupi, C Sissi, P Gratteri
The binding properties toward the human telomeric G-quadruplex of the two natural alkaloids coptisine and chelerythrine were studied using spectroscopic techniques, molecular modeling, and X-ray diffraction analysis. The results were compared with reported data for the parent compounds berberine and sanguinarine. Spectroscopic studies showed modest, but different rearrangements of the DNA-ligand complexes, which can be explained considering particular stereochemical features for these alkaloids, in spite of the similarity of their skeletons...
November 17, 2017: Journal of Natural Products
https://www.readbyqxmd.com/read/29147941/artificial-bio-nanomachines-based-on-protein-needles-derived-from-bacteriophage-t4
#4
REVIEW
Hiroshi Inaba, Takafumi Ueno
Bacteriophage T4 is a natural bio-nanomachine which achieves efficient infection of host cells via cooperative motion of specific three-dimensional protein architectures. The relationships between the protein structures and their dynamic functions have recently been clarified. In this review we summarize the design principles for fabrication of nanomachines using the component proteins of bacteriophage T4 based on these recent advances. We focus on the protein needle known as gp5, which is located at the center of the baseplate at the end of the contractile tail of bacteriophage T4...
November 16, 2017: Biophysical Reviews
https://www.readbyqxmd.com/read/29147831/plants-of-brazilian-restingas-with-tripanocide-activity-against-trypanosoma-cruzi-strains
#5
Robson Xavier Faria, André Luis Almeida Souza, Barbara Lima, Luis Armando Candido Tietbohl, Caio Pinho Fernandes, Raquel Rodrigues Amaral, Bettina Monika Ruppelt, Marcelo Guerra Santos, Leandro Rocha
Chagas disease is caused by the Trypanosoma cruzi affecting millions of people, and widespread throughout Latin America. This disease exhibits a problematic chemotherapy. Benznidazole, which is the drug currently used as standard treatment, lamentably evokes several adverse reactions. Among other options, natural products have been tested to discover a novel therapeutic drug for this disease. A lot of plants from the Brazilian flora did not contain studies about their biological effects. Restinga de Jurubatiba from Brazil is a sandbank ecosystem poorly studied in relation to plant biological activity...
November 17, 2017: Journal of Bioenergetics and Biomembranes
https://www.readbyqxmd.com/read/29147652/identification-of-potent-chloride-intracellular-channel-protein-1-inhibitors-from-traditional-chinese-medicine-through-structure-based-virtual-screening-and-molecular-dynamics-analysis
#6
Wei Wang, Minghui Wan, Dongjiang Liao, Guilin Peng, Xin Xu, Weiqiang Yin, Guixin Guo, Funeng Jiang, Weide Zhong, Jianxing He
Chloride intracellular channel 1 (CLIC1) is involved in the development of most aggressive human tumors, including gastric, colon, lung, liver, and glioblastoma cancers. It has become an attractive new therapeutic target for several types of cancer. In this work, we aim to identify natural products as potent CLIC1 inhibitors from Traditional Chinese Medicine (TCM) database using structure-based virtual screening and molecular dynamics (MD) simulation. First, structure-based docking was employed to screen the refined TCM database and the top 500 TCM compounds were obtained and reranked by X-Score...
2017: BioMed Research International
https://www.readbyqxmd.com/read/29147541/dearomatization-of-electron-poor-six-membered-n-heterocycles-through-3-2-annulation-with-aminocyclopropanes
#7
Johannes Preindl, Shyamal Chakrabarty, Jérôme Waser
Many abundant and highly bioactive natural alkaloids contain an indolizidine skeleton. A simple, high yielding method to synthesize this scaffold from N-heterocycles was developed. A wide range of pyridines, quinolines and isoquinolines reacted with donor-acceptor (DA)-aminocyclopropanes via an ytterbium(iii) catalyzed [3 + 2] annulation reaction to give tetrahydroindolizine derivatives. The products were obtained with high diastereoselectivities (dr > 20 : 1) as anti-isomers. Additionally, the formed aminals could be easily converted into secondary and tertiary amines through iminium formation followed by reduction or nucleophile addition...
October 1, 2017: Chemical Science
https://www.readbyqxmd.com/read/29146943/cryopreservation-of-human-mesenchymal-stem-cells-in-an-allogeneic-bioscaffold-based-on-platelet-rich-plasma-and-synovial-fluid
#8
Haritz Gurruchaga, Laura Saenz Del Burgo, Ane Garate, Diego Delgado, Pello Sanchez, Gorka Orive, Jesús Ciriza, Mikel Sanchez, Jose Luis Pedraz
Transplantation of mesenchymal stem cells (MSCs) has emerged as an alternative strategy to treat knee osteoarthritis. In this context, MSCs derived from synovial fluid could provide higher chondrogenic and cartilage regeneration, presenting synovial fluid as an appropriate MSCs source. An allogeneic and biomimetic bioscaffold composed of Platelet Rich Plasma and synovial fluid that preserve and mimics the natural environment of MSCs isolated from knee has also been developed. We have optimized the cryopreservation of knee-isolated MSCs embedded within the aforementioned biomimetic scaffold, in order to create a reserve of young autologous embedded knee MSCs for future clinical applications...
November 16, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29143524/injectable-shear-thinning-caso4-fgf-18-incorporated-chitin-plga-hydrogels-enhances-bone-regeneration-in-mice-cranial-bone-defect-model
#9
Sivashanmugam Amirthalingam, Pornkawee Charoenlarp, Sankar Deepthi, ArunKumar Rajendran, Shantikumar V Nair, Sachiko Iseki, Rangasamy Jayakumar
For craniofacial bone regeneration, shear thinning injectable hydrogels are favoured over conventional scaffolds due to their improved defect margin adaptability, easier handling and ability to be injected manually into deeper tissues. The most accepted method, after autografting, is the use of recombinant human BMP-2; however, complications such as inter-individual variations, edema, and poor cost-efficiency in supraphysiological doses have been reported. The endogenous synthesis of BMP-2 is desirable, and a molecule which induces this is FGF-18 since it can up-regulate the BMP-2 expression and positively modulate other osteogenic genes...
November 16, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/29143270/an-efficient-computer-aided-structural-elucidation-strategy-for-mixtures-using-an-iterative-dynamic-programming-algorithm
#10
Bo-Han Su, Meng-Yu Shen, Yeu-Chern Harn, San-Yuan Wang, Alioune Schurz, Chieh Lin, Olivia A Lin, Yufeng J Tseng
The identification of chemical structures in natural product mixtures is an important task in drug discovery but is still a challenging problem, as structural elucidation is a time-consuming process and is limited by the available mass spectra of known natural products. Computer-aided structure elucidation (CASE) strategies seek to automatically propose a list of possible chemical structures in mixtures by utilizing chromatographic and spectroscopic methods. However, current CASE tools still cannot automatically solve structures for experienced natural product chemists...
November 15, 2017: Journal of Cheminformatics
https://www.readbyqxmd.com/read/29140252/generation-of-genipin-cross-linked-fibrin-agarose-hydrogels-tissue-like-models-for-tissue-engineering-applications
#11
Fernando Campos, Ana Belen Bonhame-Espinosa, Gerson Vizcaino, Ismael Angel Rodriguez, Daniel Durand-Herrera, Modesto T Lopez-Lopez, Indalecio Sánchez-Montesinos, Miguel Alaminos, María Del Carmen Sánchez-Quevedo, Víctor Carriel
Generation of biomimetic and biocompatible artificial tissues is the basic research objective for tissue engineering (TE). In this sense, the biofabrication of scaffolds that resemble the tissues' extracellular matrix (ECM) is an essential aim in this field. Uncompressed and nanostructured fibrin-agarose hydrogels (FAH and NFAH respectively) emerged as promising scaffold in TE, but its structure and biomechanical properties must be improved in order to broad their TE applications. Here we generated and characterized novel membrane-like models with increased structural and biomechanical properties based on the chemical cross-linking of FAH and NFAH with genipin (GP at 0...
November 15, 2017: Biomedical Materials
https://www.readbyqxmd.com/read/29138282/trajectory-analysis-unveils-reelin-s-role-in-the-directed-migration-of-granule-cells-in-the-dentate-gyrus
#12
Shaobo Wang, Bianka Brunne, Shanting Zhao, Xuejun Chai, Jiawei Li, Jeremie Lau, Antonio Virgilio Failla, Bernd Zobiak, Mirjam Sibbe, Gary L Westbrook, David Lutz, Michael Frotscher
Reelin controls neuronal migration and layer formation. Previous studies in reeler mice deficient in Reelin focused on the result of the developmental process in fixed tissue sections. It has remained unclear whether Reelin affects the migratory process, migration directionality or migrating neurons guided by the radial glial scaffold. Moreover, Reelin has been regarded as an attractive signal, since newly generated neurons migrate toward the Reelin-containing marginal zone. Conversely, Reelin might be a stop signal, since migrating neurons in reeler, but not in wild-type mice, invade the marginal zone...
November 14, 2017: Journal of Neuroscience: the Official Journal of the Society for Neuroscience
https://www.readbyqxmd.com/read/29137937/design-synthesis-and-evaluation-of-3-arylidene-azetidin-2-ones-as-potential-antifungal-agents-against-alternaria-solani-sorauer
#13
Wang Delong, Wu Yongling, Wang Lanying, Feng Juntao, Zhang Xing
A new concise and facile method was explored to synthesize a collection of new 3-arylidene azetidin-2-ones, which could be regarded as the derivatives of the hybrid scaffold of bioactive natural cinnamamide and heterocycle azetidi-2-one. The structures of the synthesized compounds were characterized by (1)H, (13)C NMR, and MS; and their antifungal activity were evaluated against Alternaria solani Sorauer. These antifungal data were subjected to a quantitative structure-activity relationship (QSAR) analysis using Codessa software on the basis of the results from B3LYP/6-31G(d,p) quantum calculations...
November 4, 2017: Bioorganic & Medicinal Chemistry
https://www.readbyqxmd.com/read/29136547/natural-products-used-as-a-chemical-library-for-protein-protein-interaction-targeted-drug-discovery
#14
Xuemei Jin, Kyungro Lee, Nam Hee Kim, Hyun Sil Kim, Jong In Yook, Jiwon Choi, Kyoung Tai No
Protein-protein interactions (PPIs), which are essential for cellular processes, have been recognized as attractive therapeutic targets. Therefore, the construction of a PPI-focused chemical library is an inevitable necessity for future drug discovery. Natural products have been used as traditional medicines to treat human diseases for millennia; in addition, their molecular scaffolds have been used in diverse approved drugs and drug candidates. The recent discovery of the ability of natural products to inhibit PPIs led us to use natural products as a chemical library for PPI-targeted drug discovery...
October 27, 2017: Journal of Molecular Graphics & Modelling
https://www.readbyqxmd.com/read/29134809/ru-ii-catalyzed-c-h-amidation-of-indoline-at-the-c7-position-using-dioxazolone-as-an-amidating-agent-synthesis-of-7-amino-indoline-scaffold
#15
Akshay Ekanath Hande, Kandikere Ramaiah Prabhu
Ru(II)-Catalyzed C-H amidation of indoline at the C-7 position en route for synthesizing 7-amino indole scaffold has been achieved using dioxazolone, which is an environmentally benign amidating reagent. This protocol paves the way for synthesizing a variety of 7-amino indole derivatives in excellent yields at ambient reaction conditions. The readily cleavable amide group has been utilized as a directing group for the amidation. The derivatives of 7-amino indole are synthetically useful for accessing a variety of natural products, drug molecules, and biologically active compounds...
November 14, 2017: Journal of Organic Chemistry
https://www.readbyqxmd.com/read/29134655/protein-components-of-postsynaptic-density-lattice-a-backbone-structure-for-type-i-excitatory-synapses
#16
Tatsuo Suzuki, Kiyokazu Kametani, Weiheng Guo, Weidong Li
It is essential to study the molecular architecture of postsynaptic density (PSD) to understand the molecular mechanism underlying the dynamic nature of PSD, one of the bases of synaptic plasticity. A well-known model for the architecture of PSD of type I excitatory synapses basically comprises of several scaffolding proteins (scaffold protein model). On the contrary, "PSD lattice" observed through electron microscopy has been considered a basic backbone of type I PSDs. However, major constituents of the PSD lattice and the relationship between the PSD lattice and the scaffold protein model, are unknown...
November 14, 2017: Journal of Neurochemistry
https://www.readbyqxmd.com/read/29133927/random-protein-sequences-can-form-defined-secondary-structures-and-are-well-tolerated-in-vivo
#17
Vyacheslav Tretyachenko, Jiří Vymětal, Lucie Bednárová, Vladimír Kopecký, Kateřina Hofbauerová, Helena Jindrová, Martin Hubálek, Radko Souček, Jan Konvalinka, Jiří Vondrášek, Klára Hlouchová
The protein sequences found in nature represent a tiny fraction of the potential sequences that could be constructed from the 20-amino-acid alphabet. To help define the properties that shaped proteins to stand out from the space of possible alternatives, we conducted a systematic computational and experimental exploration of random (unevolved) sequences in comparison with biological proteins. In our study, combinations of secondary structure, disorder, and aggregation predictions are accompanied by experimental characterization of selected proteins...
November 13, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29133624/histopathological-histomorphometrical-and-radiological-evaluations-of-hydroxyapatite-bioactive-glass-and-fluorapatite-bioactive-glass-nanocomposite-foams-as-cell-scaffolds-in-rat-tibia-an-in-vivo-study
#18
Maryam Seyedmajidi, Sina Haghanifar, Karimollah Hajian-Tilaki, Seyedali Seyedmajidi
Bone defects are common and persistent problems in clinical orthopedics and dentistry. The development of synthetic reconstruction materials is essential owing to the restricted access to natural bone grafts, disease transmission risks, surgical costs, donor-site morbidity, infections, and immune response-related complications. Accordingly, the aim of the present study was to evaluate the histopathological, histomorphometrical, and radiological characteristics of composite foams containing hydroxyapatite/bioactive glass (HA/BG) and fluorapatite/bioactive glass (FA/BG) as cell scaffolds in rat tibia reconstruction...
November 14, 2017: Biomedical Materials
https://www.readbyqxmd.com/read/29132046/stress-relaxing-hyaluronic-acid-collagen-hydrogels-promote-cell-spreading-fiber-remodeling-and-focal-adhesion-formation-in-3d-cell-culture
#19
Junzhe Lou, Ryan Stowers, Sungmin Nam, Yan Xia, Ovijit Chaudhuri
The physical and architectural cues of the extracellular matrix (ECM) play a critical role in regulating important cellular functions such as spreading, migration, proliferation, and differentiation. Natural ECM is a complex viscoelastic scaffold composed of various distinct components that are often organized into a fibrillar microstructure. Hydrogels are frequently used as synthetic ECMs for 3D cell culture, but are typically elastic, due to covalent crosslinking, and non-fibrillar. Recent work has revealed the importance of stress relaxation in viscoelastic hydrogels in regulating biological processes such as spreading and differentiation, but these studies all utilize synthetic ECM hydrogels that are non-fibrillar...
November 6, 2017: Biomaterials
https://www.readbyqxmd.com/read/29130595/naphthoquinones-a-continuing-source-for-discovery-of-therapeutic-antineoplastic-agents
#20
REVIEW
Han-Yue Qiu, Peng-Fei Wang, Hong-Yan Lin, Cheng-Yi Tang, Hai-Liang Zhu, Yong-Hua Yang
Naturally occurring naphthoquinones, usually in forms of botanical extracts, have been implicated with human life since ancient time, far earlier than their isolation and identification in modern era. The long use history of naphthoquinones has witnessed their functional shift from the original purposes as dyes and ornaments towards medicinal benefits. Hitherto, numerous studies have been carried out to elucidate the pharmacological profile of both natural and artificial naphthoquinones. A number of entities have been identified with promising therapeutic potential...
November 11, 2017: Chemical Biology & Drug Design
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