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Adipose drived stem cell

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https://www.readbyqxmd.com/read/28521687/diet-and-contaminants-driving-the-rise-to-obesity-epidemics
#1
Agostino Di Ciaula, Piero Portincasa
The obesity epidemic is spreading worldwide without reversal trend and despite specific policies oriented to dietary habits and lifestyle, which seem to have modest effects. Genetic factors only partly explain the rise, whereas environmental factors seem to play a key role, mainly by gene-environment interactions through epigenetic mechanisms. A number of animal and human studies point to maternal diet, intestinal microbiota and chemicals introduced as contaminants with food, all factors able to increase the risk of obesity...
May 17, 2017: Current Medicinal Chemistry
https://www.readbyqxmd.com/read/28439492/leptin-signaling-and-cancer-chemoresistance-perspectives
#2
EDITORIAL
Pierre V Candelaria, Antonio Rampoldi, Adriana Harbuzariu, Ruben R Gonzalez-Perez
Obesity is a major health problem and currently is endemic around the world. Obesity is a risk factor for several different types of cancer, significantly promoting cancer incidence, progression, poor prognosis and resistance to anti-cancer therapies. The study of this resistance is critical as development of chemoresistance is a serious drawback for the successful and effective drug-based treatments of cancer. There is increasing evidence that augmented adiposity can impact on chemotherapeutic treatment of cancer and the development of resistance to these treatments, particularly through one of its signature mediators, the adipokine leptin...
April 10, 2017: World Journal of Clinical Oncology
https://www.readbyqxmd.com/read/28376564/adipogenic-differentiation-of-mesenchymal-stem-cells-alters-their-immunomodulatory-properties-in-a-tissue-specific-manner
#3
Hafsa Munir, Lewis S C Ward, Lozan Sheriff, Samuel Kemble, Saba Nayar, Francesca Barone, Gerard B Nash, Helen M McGettrick
Chronic inflammation is associated with formation of ectopic fat deposits that might represent damage-induced aberrant mesenchymal stem cell (MSC) differentiation. Such deposits are associated with increased levels of inflammatory infiltrate and poor prognosis. Here we tested the hypothesis that differentiation from MSC to adipocytes in inflamed tissue might contribute to chronicity through loss of immunomodulatory function. We assessed the effects of adipogenic differentiation of MSC isolated from bone marrow or adipose tissue on their capacity to regulate neutrophil recruitment by endothelial cells and compared the differentiated cells to primary adipocytes from adipose tissue...
April 4, 2017: Stem Cells
https://www.readbyqxmd.com/read/28333993/integrated-transcriptomic-and-proteomic-analysis-of-the-molecular-cargo-of-extracellular-vesicles-derived-from-porcine-adipose-tissue-derived-mesenchymal-stem-cells
#4
Alfonso Eirin, Xiang-Yang Zhu, Amrutesh S Puranik, John R Woollard, Hui Tang, Surendra Dasari, Amir Lerman, Andre J van Wijnen, Lilach O Lerman
BACKGROUND: Mesenchymal stromal/stem cell (MSC) transplantation is a promising therapy for tissue regeneration. Extracellular vesicles (EVs) released by MSCs act as their paracrine effectors by delivering proteins and genetic material to recipient cells. To assess how their cargo mediates biological processes that drive their therapeutic effects, we integrated miRNA, mRNA, and protein expression data of EVs from porcine adipose tissue-derived MSCs. METHODS: Simultaneous expression profiles of miRNAs, mRNAs, and proteins were obtained by high-throughput sequencing and LC-MS/MS proteomic analysis in porcine MSCs and their daughter EVs (n = 3 each)...
2017: PloS One
https://www.readbyqxmd.com/read/28270600/diverse-regulation-of-mammary-epithelial-growth-and-branching-morphogenesis-through-noncanonical-wnt-signaling
#5
Kai Kessenbrock, Prestina Smith, Sander Christiaan Steenbeek, Nicholas Pervolarakis, Raj Kumar, Yasuhiro Minami, Andrei Goga, Lindsay Hinck, Zena Werb
The mammary gland consists of an adipose tissue that, in a process called branching morphogenesis, is invaded by a ductal epithelial network comprising basal and luminal epithelial cells. Stem and progenitor cells drive mammary growth, and their proliferation is regulated by multiple extracellular cues. One of the key regulatory pathways for these cells is the β-catenin-dependent, canonical wingless-type MMTV integration site family (WNT) signaling pathway; however, the role of noncanonical WNT signaling within the mammary stem/progenitor system remains elusive...
March 21, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28259801/effect-of-tetrahedral-dna-nanostructures-on-osteogenic-differentiation-of-mesenchymal-stem-cells-via-activation-of-the-wnt-%C3%AE-catenin-signaling-pathway
#6
Xiao-Ru Shao, Shi-Yu Lin, Qiang Peng, Si-Rong Shi, Xiao-Long Li, Tao Zhang, Yun-Feng Lin
Adipose-derived stem cells (ADSCs) are considered to be ideal stem cell sources for bone regeneration owing to their ability to differentiate into osteo-like cells. Therefore, they have attracted increasing attention in recent years. Tetrahedral DNA nanostructures (TDNs), a new type of DNA-based biomaterials, have shown great potential for biomedical applications. In the present work, we aimed to investigate the role played by TDNs in osteogenic differentiation and proliferation of ADSCs and tried to explore if the canonical Wnt signal pathway could be the vital biological mechanism driving these cellular responses...
March 1, 2017: Nanomedicine: Nanotechnology, Biology, and Medicine
https://www.readbyqxmd.com/read/28186685/concise-review-fat-and-furious-harnessing-the-full-potential-of-adipose-derived-stromal-vascular-fraction
#7
Jordan A Dykstra, Tiffany Facile, Ryan J Patrick, Kevin R Francis, Samuel Milanovich, Jill M Weimer, Daniel J Kota
Due to their capacity to self-renew, proliferate and generate multi-lineage cells, adult-derived stem cells offer great potential for use in regenerative therapies to stop and/or reverse degenerative diseases such as diabetes, heart failure, Alzheimer's disease and others. However, these subsets of cells can be isolated from different niches, each with differing potential for therapeutic applications. The stromal vascular fraction (SVF), a stem cell enriched and adipose-derived cell population, has garnered interest as a therapeutic in regenerative medicine due to its ability to secrete paracrine factors that accelerate endogenous repair, ease of accessibility and lack of identified major adverse effects...
April 2017: Stem Cells Translational Medicine
https://www.readbyqxmd.com/read/28093366/strategy-for-constructing-vascularized-adipose-units-in-poly-l-glutamic-acid-hydrogel-porous-scaffold-through-inducing-in-situ-formation-of-ascs-spheroids
#8
Kunxi Zhang, Li Song, Jia Wang, Shifeng Yan, Guifei Li, Lei Cui, Jingbo Yin
Vascularization is of great importance to adipose tissue regeneration. Here we introduced a paradigm that using scaffold to induce ASC spheroids, so to promote vascularized adipose tissue regeneration. Poly (l-glutamic acid) (PLGA) was activated by EDC, followed by being cross-linked by Adipic dihydrazide (ADH) to form a homogeneous hydrogel. Lyophilization was then carried out to create porous structure. The PLGA hydrogel scaffold possessed a significant swollen hydrophilic network to weaken cell-scaffold adhesion but drive ASCs to aggregate to form spheroids...
January 16, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/28004524/a-cell-based-self-assembly-approach-for-the-production-of-human-osseous-tissues-from-adipose-derived-stromal-stem-cells
#9
Todd Galbraith, William P Clafshenkel, Fabien Kawecki, Camille Blanckaert, Benoit Labbé, Michel Fortin, François A Auger, Julie Fradette
Achieving optimal bone defect repair is a clinical challenge driving intensive research in the field of bone tissue engineering. Many strategies focus on seeding graft materials with progenitor cells prior to in vivo implantation. Given the benefits of closely mimicking tissue structure and function with natural materials, the authors hypothesize that under specific culture conditions, human adipose-derived stem/stromal cells (hASCs) can solely be used to engineer human reconstructed osseous tissues (hROTs) by undergoing osteoblastic differentiation with concomitant extracellular matrix production and mineralization...
December 22, 2016: Advanced Healthcare Materials
https://www.readbyqxmd.com/read/27888602/a-glycovariant-of-human-cd44-is-characteristically-expressed-on-human-mesenchymal-stem-cells
#10
Gisela Pachón-Peña, Conor Donnelly, Catalina Ruiz-Cañada, Adam Katz, Sonia Fernández-Veledo, Joan Vendrell, Robert Sackstein
The clinical effectiveness of systemically administered human mesenchymal stem cells (hMSCs) depends on their capacity to engage vascular endothelium. hMSCs derived from bone marrow (BM-hMSCs) natively lack endothelial binding capacity, but express a CD44 glycovariant containing N-linked sialyllactosamines that can be α(1,3)-fucosylated using fucosyltransferase-VI (FTVI) to enforce sLe(X) decorations, thereby creating hematopoietic cell E-/L-selectin ligand (HCELL). HCELL expression programs potent shear-resistant adhesion of circulating cells to endothelial beds expressing E-selectin...
April 2017: Stem Cells
https://www.readbyqxmd.com/read/27871224/insights-into-the-human-mesenchymal-stromal-stem-cell-identity-through-integrative-transcriptomic-profiling
#11
Beatriz Roson-Burgo, Fermin Sanchez-Guijo, Consuelo Del Cañizo, Javier De Las Rivas
BACKGROUND: Mesenchymal Stromal/Stem Cells (MSCs), isolated under the criteria established by the ISCT, still have a poorly characterized phenotype that is difficult to distinguish from similar cell populations. Although the field of transcriptomics and functional genomics has quickly grown in the last decade, a deep comparative analysis of human MSCs expression profiles in a meaningful cellular context has not been yet performed. There is also a need to find a well-defined MSCs gene-signature because many recent biomedical studies show that key cellular interaction processes (i...
November 21, 2016: BMC Genomics
https://www.readbyqxmd.com/read/27820963/adeno-associated-virus-5-transduces-adipose-derived-stem-cells-with-greater-efficacy-than-other-adeno-associated-viral-serotypes
#12
Priyanka Sharma, Sunishka M Wimalawansa, Gregory C Gould, R Michael Johnson, Katherine J D A Excoffon
Adipose-derived stem cells (ASCs) have shown potential in the treatment of a myriad of diseases; however, infusion of cells alone is unlikely to provide the full range of potential therapeutic applications. Transient genetic manipulation of ASCs could increase their repair and regeneration characteristics in a disease-specific context, essentially transforming them into drug-eluting depots. The goal of this study was to determine the optimal parameters necessary to transduce ASCs with recombinant adeno-associated virus (rAAV), an approved gene therapy vector that has never been associated with disease...
December 2016: Human Gene Therapy Methods
https://www.readbyqxmd.com/read/27746098/skin-adipocyte-stem-cell-self-renewal-is-regulated-by-a-pdgfa-akt-signaling-axis
#13
Guillermo C Rivera-Gonzalez, Brett A Shook, Johanna Andrae, Brandon Holtrup, Katherine Bollag, Christer Betsholtz, Matthew S Rodeheffer, Valerie Horsley
Tissue growth and maintenance requires stem cell populations that self-renew, proliferate, and differentiate. Maintenance of white adipose tissue (WAT) requires the proliferation and differentiation of adipocyte stem cells (ASCs) to form postmitotic, lipid-filled mature adipocytes. Here we use the dynamic adipogenic program that occurs during hair growth to uncover an unrecognized regulator of ASC self-renewal and proliferation, PDGFA, which activates AKT signaling to drive and maintain the adipogenic program in the skin...
December 1, 2016: Cell Stem Cell
https://www.readbyqxmd.com/read/27687795/improved-calvarial-bone-repair-by-hascs-engineered-with-cre-loxp-based-baculovirus-conferring-prolonged-bmp-2-and-mir-148b-co-expression
#14
Kuei-Chang Li, Shih-Chun Lo, Li-Yu Sung, Ya-Hsin Liao, Yu-Han Chang, Yu-Chen Hu
Repairing large calvarial bone defects remains a challenging task. Previously, it was discovered that that miR-148b, when acting in concert with bone morphogenetic protein 2 (BMP-2), enhanced the osteogenesis of human adipose-derived stem cells (hASCs) and improved calvarial bone healing in nude mice. However, the molecular target of miR-148b remained elusive. Here it is revealed that miR-148b directly targets NOG, whose gene product (noggin) is an antagonist to BMPs and negatively regulates BMP-induced osteogenic differentiation and bone formation...
September 30, 2016: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/27404713/secretome-from-resident-cardiac-stromal-cells-stimulates-proliferation-cardiomyogenesis-and-angiogenesis-of-progenitor-cells
#15
Thamile Luciane Reus, Anny Waloski Robert, Marise Brenner Affonso Da Costa, Alessandra Melo de Aguiar, Marco Augusto Stimamiglio
In the heart, tissue-derived signals play a central role on recruiting/activating stem cell sources to induce cardiac lineage specification for maintenance of tissue homeostasis and repair. Cardiac resident stromal cells (CRSCs) may play a pivotal role in cardiac repair throughout their secretome. Here, we performed the characterization of CRSCs and their secretome by analyzing the composition of their culture-derived extracellular matrix (ECM) and conditioned medium (CM) and by investigating their potential effect on adipose-derived stem cell (ADSC) and progenitor cell behavior...
October 15, 2016: International Journal of Cardiology
https://www.readbyqxmd.com/read/27390514/an-ex-vivo-gene-therapy-approach-in-x-linked-retinoschisis
#16
Abu E Bashar, Andrew L Metcalfe, Ishaq A Viringipurampeer, Anat Yanai, Cheryl Y Gregory-Evans, Kevin Gregory-Evans
PURPOSE: X-linked retinoschisis (XLRS) is juvenile-onset macular degeneration caused by haploinsufficiency of the extracellular cell adhesion protein retinoschisin (RS1). RS1 mutations can lead to either a non-functional protein or the absence of protein secretion, and it has been established that extracellular deficiency of RS1 is the underlying cause of the phenotype. Therefore, we hypothesized that an ex vivo gene therapy strategy could be used to deliver sufficient extracellular RS1 to reverse the phenotype seen in XLRS...
2016: Molecular Vision
https://www.readbyqxmd.com/read/27345526/serum-mirna-signatures-are-indicative-of-skeletal-fractures-in-postmenopausal-women-with-and-without-type-2-diabetes-and-influence-osteogenic-and-adipogenic-differentiation-of-adipose-tissue-derived-mesenchymal-stem-cells-in-vitro
#17
Ursula Heilmeier, Matthias Hackl, Susanna Skalicky, Sylvia Weilner, Fabian Schroeder, Klemens Vierlinger, Janina M Patsch, Thomas Baum, Eleni Oberbauer, Iryna Lobach, Andrew J Burghardt, Ann V Schwartz, Johannes Grillari, Thomas M Link
Standard DXA measurements, including Fracture Risk Assessment Tool (FRAX) scores, have shown limitations in assessing fracture risk in Type 2 Diabetes (T2D), underscoring the need for novel biomarkers and suggesting that other pathomechanisms may drive diabetic bone fragility. MicroRNAs (miRNAs) are secreted into the circulation from cells of various tissues proportional to local disease severity and were recently found to be crucial to bone homeostasis and T2D. Here, we studied, if and which circulating miRNAs or combinations of miRNAs can discriminate best fracture status in a well-characterized study of diabetic bone disease and postmenopausal osteoporosis (n = 80 postmenopausal women)...
December 2016: Journal of Bone and Mineral Research: the Official Journal of the American Society for Bone and Mineral Research
https://www.readbyqxmd.com/read/27329656/a-dermal-equivalent-developed-from-adipose-derived-stem-cells-and-electrospun-polycaprolactone-matrix-an-in-vitro-and-in-vivo-study
#18
Vahid Bayati, Mohammad Reza Abbaspour, Fereshteh Negad Dehbashi, Niloofar Neisi, Mahmoud Hashemitabar
Polycaprolactone (PCL) is used as a material of choice for surgical sutures, wound dressings, contraceptives, fixation devices and dentistry in paramedical sciences. In addition, adipose-derived stem cells (ASCs) have been shown to be effective in the treatment of acute and chronic wounds. This study aimed to evaluate the effect of electrospun PCL fibers on keratinocyte differentiation of ASCs and wound healing. PCL solution was electrospun and characterized. Isolated and characterized ASCs were differentiated into keratinocyte-like cells on a tissue culture plate (TCP) and PCL matrices and compared...
June 21, 2016: Anatomical Science International
https://www.readbyqxmd.com/read/27245267/tonsil-derived-mesenchymal-stem-cells-promote-bone-mineralization-and-reduce-marrow-and-visceral-adiposity-in-a-mouse-model-of-senile-osteoporosis
#19
Yu-Hee Kim, Minhwa Park, Kyung-Ah Cho, Bo-Kyung Kim, Jung-Hwa Ryu, So-Youn Woo, Kyung-Ha Ryu
Osteoporosis is a disease that affects 35% women and 20% men aged more than 65 years. Reduction in bone formation and increased bone resorption are known factors that drive osteoporosis, but recent studies suggest a positive correlation between bone marrow adipose tissue (MAT) and osteoporosis. Previously, we have observed that tonsil-derived mesenchymal stem cells (T-MSCs) reduce MAT in a mouse model of bone marrow depletion. That prompted us to investigate on the senile osteoporosis to characterize the bone-forming effect, as well as MAT-reducing effect of T-MSCs...
August 1, 2016: Stem Cells and Development
https://www.readbyqxmd.com/read/27163860/biomimetic-3d-clusters-using-human-adipose-derived-mesenchymal-stem-cells-and-breast-cancer-cells-a-study-on-migration-and-invasion-of-breast-cancer-cells
#20
Min Hee Park, Boa Song, Seungpyo Hong, Sang Heon Kim, Kangwon Lee
Invasion and metastasis of cancer directly related to human death have been associated with interactions among many different types of cells and three-dimensional (3D) tissue matrices. Precise mechanisms related to cancer invasion and metastasis still remain unknown due to their complexities. Development of tumor microenvironment (TME)-mimicking system could play a key role in understanding cancer environments and in elucidating the relating phenomena and their driving forces. Here we report a facile and novel platform of 3D cancer cell-clusters using human adipose-derived mesenchymal stem cells (hASCs) and breast cancer cells (MDA-MB-231) within a collagen gel matrix to show cancer invasion in the cell and extracellular matrix (ECM)...
July 5, 2016: Molecular Pharmaceutics
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