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https://www.readbyqxmd.com/read/28440853/antibiotic-strategies-for-eradicating-pseudomonas-aeruginosa-in-people-with-cystic-fibrosis
#1
REVIEW
Simon C Langton Hewer, Alan R Smyth
BACKGROUND: Respiratory tract infection with Pseudomonas aeruginosa occurs in most people with cystic fibrosis. Once chronic infection is established, Pseudomonas aeruginosa is virtually impossible to eradicate and is associated with increased mortality and morbidity. Early infection may be easier to eradicate.This is an update of a Cochrane review first published in 2003, and previously updated in 2006, 2009 and 2014. OBJECTIVES: To determine whether antibiotic treatment of early Pseudomonas aeruginosa infection in children and adults with cystic fibrosis eradicates the organism, delays the onset of chronic infection, and results in clinical improvement...
April 25, 2017: Cochrane Database of Systematic Reviews
https://www.readbyqxmd.com/read/28438138/accuracy-of-serum-procalcitonin-for-the-diagnosis-of-sepsis-in-neonates-and-children-with-systemic-inflammatory-syndrome-a-meta-analysis
#2
Giuseppe Pontrelli, Franco De Crescenzo, Roberto Buzzetti, Alessandro Jenkner, Sara Balduzzi, Francesca Calò Carducci, Donato Amodio, Maia De Luca, Sara Chiurchiù, Elin Haf Davies, Giorgia Copponi, Alessandra Simonetti, Elena Ferretti, Valeria Di Franco, Virginia Rasi, Martina Della Corte, Luca Gramatica, Marco Ciabattini, Susanna Livadiotti, Paolo Rossi
BACKGROUND: A number of biomarkers have been studied for the diagnosis of sepsis in paediatrics, but no gold standard has been identified. Procalcitonin (PCT) was demonstrated to be an accurate biomarker for the diagnosis of sepsis in adults and showed to be promising in paediatrics. Our study reviewed the diagnostic accuracy of PCT as an early biomarker of sepsis in neonates and children with suspected sepsis. METHODS: A comprehensive literature search was carried out in Medline/Pubmed, Embase, ISI Web of Science, CINAHL and Cochrane Library, for studies assessing PCT accuracy in the diagnosis of sepsis in children and neonates with suspected sepsis...
April 24, 2017: BMC Infectious Diseases
https://www.readbyqxmd.com/read/28438086/feline-leprosy-due-to-candidatus-mycobacterium-tarwinense
#3
Carolyn R O'Brien, Richard Malik, Maria Globan, George Reppas, Christina McCowan, Janet A Fyfe
OBJECTIVES: This paper, the first in a series of three on 'feline leprosy', provides a detailed description of disease referable to Candidatus 'Mycobacterium tarwinense', the most common cause of feline leprosy in Victoria, Australia. METHODS: Cases were sourced retrospectively and prospectively for this observational study, describing clinical, geographical and molecular microbiological data for cats definitively diagnosed with Candidatus 'M tarwinense' infection...
May 2017: Journal of Feline Medicine and Surgery
https://www.readbyqxmd.com/read/28434737/short-communication-microbiological-quality-of-raw-cow-milk-and-its-association-with-herd-management-practices-in-northern-china
#4
X Y Lan, S G Zhao, N Zheng, S L Li, Y D Zhang, H M Liu, J McKillip, J Q Wang
Contamination of raw milk with bacterial pathogens is potentially hazardous to human health. The aim of this study was to evaluate the total bacteria count (TBC) and presence of pathogens in raw milk in Northern China along with the associated herd management practices. A total of 160 raw milk samples were collected from 80 dairy herds in Northern China. All raw milk samples were analyzed for TBC and pathogens by culturing. The results showed that the number of raw milk samples with TBC <2 × 10(6) cfu/mL and <1 × 10(5) cfu/mL was 146 (91...
April 20, 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28432990/influence-of-red-mud-on-soil-microbial-communities-application-and-comprehensive-evaluation-of-the-biolog-ecoplate-approach-as-a-tool-in-soil-microbiological-studies
#5
Viktória Feigl, Éva Ujaczki, Emese Vaszita, Mónika Molnár
Red mud can be applied as soil ameliorant to acidic, sandy and micronutrient deficient soils. There are still knowledge gaps regarding the effects of red mud on the soil microbial community. The Biolog EcoPlate technique is a promising tool for community level physiological profiling. This study presents a detailed evaluation of Biolog EcoPlate data from two case studies. In experiment "A" red mud from Ajka (Hungary) was mixed into acidic sandy soil in soil microcosms at 5-50 w/w%. In experiement "B" red mud soil mixture was mixed into low quality subsoil in a field experiment at 5-50 w/w%...
April 19, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28423881/effects-of-calcium-propionate-on-the-fermentation-quality-and-aerobic-stability-of-alfalfa-silage
#6
Zhi Hao Dong, Xian Jun Yuan, Ai You Wen, Seare T Desta, Tao Shao
Objective: To assess the potency of calcium propionate used as silage additive, an experiment was carried out to evaluate the effect of calcium propionate on the nitrogen transformation, fermentation quality and aerobic stability of alfalfa silages. Methods: Alfalfa was ensiled with four levels of calcium propionate (CAP; 5, 10, 15 and 20 g kg-1 of fresh weigh) in laboratory silos for 30 days. After opening, the silages were analyzed for the chemical and microbiological characteristics, and subjected to an aerobic stability test...
March 25, 2017: Asian-Australasian Journal of Animal Sciences
https://www.readbyqxmd.com/read/28417292/probiotic-strawberry-yogurts-microbiological-chemical-and-sensory-properties
#7
Tamer Turgut, Songül Cakmakci
This study was performed to determine the viability of Lactobacillus acidophilus and Bifidobacterium bifidum in yogurt made with strawberry marmalade (SM) and to examine the quality properties of probiotic yogurt. Acidity, pH, bacterial counts and sensory analysis of the yogurt samples were investigated on days 1, 3, 5, 7, 10 and 14 during storage at 4 °C. The survival rate of L. acidophilus was greater than that of B. bifidum. The viability of L. acidophilus decreased during the storage period, but B. bifidum numbers remained stable during the storage period...
April 17, 2017: Probiotics and Antimicrobial Proteins
https://www.readbyqxmd.com/read/28416464/microbiological-quality-of-water-in-a-city-with-persistent-and-recurrent-waterborne-diseases-under-tropical-sub-rural-conditions-the-case-of-kikwit-city-democratic-republic-of-the-congo
#8
Alexis B Nienie, Periyasamy Sivalingam, Amandine Laffite, Patience Ngelinkoto, Jean-Paul Otamonga, Alphonse Matand, Crispin K Mulaji, Emmanuel M Biey, Pius T Mpiana, John Poté
The availability of safe drinking water in sub-Saharan countries remains a major challenge because poor sanitation has been the cause of various outbreaks of waterborne disease due to the poor microbiological quality of water used for domestic purposes. The faecal indicator bacteria (FIB) used in the present study included Escherichia coli (E. coli) and Enterococcus (ENT). FIB and aerobic mesophilic bacteria (AMB) were quantified during July 2015 (dry season) and November 2015 (rainy season) in order to assess the quality of drinking water from wells (n=3; P1-P3), and two rivers, the River Lukemi (RLK, n=3) and River Luini (RLN, n=2) in the city of Kikwit, which is located in the province of Kwilu in the Democratic Republic of the Congo...
March 30, 2017: International Journal of Hygiene and Environmental Health
https://www.readbyqxmd.com/read/28412382/use-of-nebulized-antimicrobials-for-the-treatment-of-respiratory-infections-in-invasively-mechanically-ventilated-adults-a-position-paper-from-the-european-society-of-clinical-microbiology-and-infectious-diseases
#9
Jordi Rello, Candela Solé-Lleonart, Jean-Jacques Rouby, Jean Chastre, Stijn Blot, Garyfallia Poulakou, Charles-Edouard Luyt, Jordi Riera, Lucy B Palmer, Jose M Pereira, Tim Felton, Jayesh Dhanani, Matteo Bassetti, Tobias Welte, Jason A Roberts
With an established role in cystic fibrosis and bronchiectasis, nebulized antibiotics are increasingly being used to treat respiratory infections in critically ill invasively mechanically ventilated adult patients. Although there is limited evidence describing their efficacy and safety, in an era of need for new strategies to enhance antibiotic effectiveness because of a shortage of new agents and increases in antibiotic resistance, the potential of nebulization of antibiotics to optimize therapy is considered of high interest, particularly in patients infected with multidrug-resistant (MDR) pathogens...
April 13, 2017: Clinical Microbiology and Infection
https://www.readbyqxmd.com/read/28412378/project-management-importance-for-diagnostic-laboratories
#10
REVIEW
Antony Croxatto, Gilbert Greub
BACKGROUND: The constraints applied on diagnostic laboratories to improve both quality and productivity together with personal shortages incite laboratory managers to constantly optimize the laboratory workflows, organization and technology. These continuous modifications of the laboratories should be conducted using efficient project and change management approaches in order to maximize the opportunities to achieve the project successfully. AIM: This review aims at presenting a general overview of project management with an emphasis on selected critical aspects...
April 12, 2017: Clinical Microbiology and Infection
https://www.readbyqxmd.com/read/28412024/prevalence-of-bacterial-contamination-in-platelet-concentrates-at-the-national-center-of-blood-transfusion-mexico
#11
G Ibáñez-Cervantes, J M Bello-López, V Fernández-Sánchez, C A Domínguez-Mendoza, L I Acevedo-Alfaro
OBJECTIVES: Most common bacterial sepsis associated with transfusion is caused by contaminated Platelet Concentrates (PC). The screening of PC to detect bacterial contamination is obligatory in Mexico, and it is carried out in quality control programs. In Mexico, the identification and molecular characterization of bacterial contaminants to detect contamination sources have not been implemented due to high costs; however, it is an actual current need. MATERIAL AND METHODS: One hundred PC were randomly selected and microbiologically analyzed...
April 12, 2017: Transfusion Clinique et Biologique: Journal de la Société Française de Transfusion Sanguine
https://www.readbyqxmd.com/read/28404682/comparison-of-the-eplex%C3%A2-respiratory-pathogen-panel-with-laboratory-developed-real-time-pcr-for-the-detection-of-respiratory-pathogens
#12
R H T Nijhuis, D Guerendiain, E C J Claas, K E Templeton
Infections of the respiratory tract can be caused by a diversity of pathogens, both viral and bacterial. Rapid microbiological diagnosis ensures appropriate antimicrobial therapy as well as effective implementation of isolation precautions. The ePlex® Respiratory Pathogen Panel (RP panel) is a novel molecular based assay, developed by GenMark Diagnostics Inc. (Carlsbad, California), to be performed within a single cartridge for the diagnosis of 25 respiratory pathogens (viral and bacterial). The objective of this study was to compare the performance of the RP panel with laboratory developed real-time PCR assays, using a variety of previously collected clinical respiratory specimens...
April 12, 2017: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/28400009/autochthonous-lactic-acid-bacteria-with-probiotic-aptitudes-as-starter-cultures-for-fish-based-products
#13
Barbara Speranza, Angela Racioppo, Luciano Beneduce, Antonio Bevilacqua, Milena Sinigaglia, Maria Rosaria Corbo
This study focused on the selection of lactic starters with probiotic properties for the production of fermented fish-products by the use of a multivariate approach (Cluster Analysis and Principal Component Analysis). Seventy-five isolates were recovered from fish intestinal microbiota and characterized by evaluating phenotypical, technological and probiotic traits; the most promising isolates were molecularly identified and then used into fish fermented sausage production. Namely, data from technological characterization were modelled through Growth Index and used as input to run a preliminary selection...
August 2017: Food Microbiology
https://www.readbyqxmd.com/read/28400006/microbiological-quality-of-selected-ready-to-eat-leaf-vegetables-sprouts-and-non-pasteurized-fresh-fruit-vegetable-juices-including-the-presence-of-cronobacter-spp
#14
Anna Berthold-Pluta, Monika Garbowska, Ilona Stefańska, Antoni Pluta
Bacteria of the genus Cronobacter are emerging food-borne pathogens. Foods contaminated with Cronobacter spp. may pose a risk to infants or adults with suppressed immunity. This study was aimed at determining the microbiological quality of ready-to-eat (RTE) plant-origin food products available on the Polish market with special emphasis on the prevalence of Cronobacter genus bacteria. Analyses were carried out on 60 samples of commercial RTE type plant-origin food products, including: leaf vegetables (20 samples), sprouts (20 samples) and non-pasteurized vegetable, fruit and fruit-vegetable juices (20 samples)...
August 2017: Food Microbiology
https://www.readbyqxmd.com/read/28399992/microbial-quality-of-irrigation-water-used-in-leafy-green-production-in-southern-brazil-and-its-relationship-with-produce-safety
#15
Luana Tombini Decol, Letícia Sopeña Casarin, Claudia Titze Hessel, Ana Carolina Fösch Batista, Ana Allende, Eduardo César Tondo
Irrigation water has been recognized as an important microbial risk factor for fruits and vegetables in many production areas, but there is still a lack of information about how the microbiological quality of different irrigation water sources and climatic conditions influence the safety of vegetables produced in Brazil. This study evaluated the distribution of generic E. coli and the prevalence of E. coli O157:H7 in two different water sources (ponds and streams bordering farmlands and urban areas) used for irrigation and on commercially produced lettuces in Southern Brazil...
August 2017: Food Microbiology
https://www.readbyqxmd.com/read/28399144/microbial-quality-of-agricultural-water-in-central-florida
#16
Zeynal Topalcengiz, Laura K Strawn, Michelle D Danyluk
The microbial quality of water that comes into the edible portion of produce is believed to directly relate to the safety of produce, and metrics describing indicator organisms are commonly used to ensure safety. The US FDA Produce Safety Rule (PSR) sets very specific microbiological water quality metrics for agricultural water that contacts the harvestable portion of produce. Validation of these metrics for agricultural water is essential for produce safety. Water samples (500 mL) from six agricultural ponds were collected during the 2012/2013 and 2013/2014 growing seasons (46 and 44 samples respectively, 540 from all ponds)...
2017: PloS One
https://www.readbyqxmd.com/read/28392724/an-overview-of-the-european-organization-for-external-quality-assurance-providers-in-laboratory-medicine-eqalm
#17
REVIEW
Anne Stavelin, Xavier Albe, Piet Meijer, Erika Sarkany, Finlay MacKenzie
The European Organisation for External Quality Assurance Providers in Laboratory Medicine (EQALM) was founded in 1996 and currently has members from 29 European countries and 6 countries from outside Europe. EQALM provides a forum for co-operation and exchange of knowledge on quality-related matters in laboratory medicine, especially with regard to external quality assessment (EQA) programs in Europe. In addition, EQALM represent the EQA providers in laboratory medicine at European level vis-ŕ-vis political, professional, scientific and other bodies, including patients' organisations...
February 15, 2017: Biochemia Medica: časopis Hrvatskoga Društva Medicinskih Biokemičara
https://www.readbyqxmd.com/read/28391475/a-study-on-prevalence-of-microbial-contamination-on-the-surface-of-raw-salad-vegetables
#18
Sujeet Kumar Mritunjay, Vipin Kumar
The present work evaluates the microbiological quality of raw salad vegetables (RSV) consumed in Dhanbad city, India. A total of 480 samples of 8 different raw salad vegetables from local market were examined for overall microbial quality in terms of aerobic mesophilic, psychrotrophic counts, yeast, mould and total coliform levels. E. coli O157:H7, Listeria monocytogenes and Salmonella sp. were detected by real-time polymerase chain reaction (qPCR) subsequent to isolation. Results showed that all the samples were found positive for total coliform; however, E...
May 2017: 3 Biotech
https://www.readbyqxmd.com/read/28389992/detection-of-biofilms-in-biopsies-from-chronic-rhinosinusitis-patients-in-vitro-biofilm-forming-ability-and-antimicrobial-susceptibility-testing-in-biofilm-mode-of-growth-of-isolated-bacteria
#19
Mariagrazia Di Luca, Elena Navari, Semih Esin, Melissa Menichini, Simona Barnini, Andrej Trampuz, Augusto Casani, Giovanna Batoni
Chronic rhinosinusitis (CRS) is the most common illness among chronic disorders that remains poorly understood from a pathogenic standpoint and has a significant impact on patient quality of life, as well as healthcare costs. Despite being widespread, little is known about the etiology of the CRS. Recent evidence, showing the presence of biofilms within the paranasal sinuses, suggests a role for biofilm in the pathogenesis. To elucidate the role of biofilm in the pathogenesis of CRS, we assessed the presence of biofilm at the infection site and the ability of the aerobic flora isolated from CRS patients to form biofilm in vitro...
April 8, 2017: Advances in Experimental Medicine and Biology
https://www.readbyqxmd.com/read/28379372/the-microbiological-landscape-of-anaerobic-infections-in-hidradenitis-suppurativa-a-prospective-metagenomic-study
#20
Hélène Guet-Revillet, Jean-Philippe Jais, Marie-Noelle Ungeheuer, Hélène Coignard-Biehler, Sabine Duchatelet, Maïa Delage, Thi Lam, Alain Hovnanian, Olivier Lortholary, Xavier Nassif, Aude Nassif, Olivier Join-Lambert
Background.: Hidradenitis suppurativa (HS) is a frequent and severe disease of the skin, characterized by recurrent or chronic skinfolds suppurative lesions with a high impact on quality of life. Although considered inflammatory, antimicrobial treatments can improve or lead to clinical remission of HS, suggesting triggering microbial factors. Indeed, mixed anaerobic microbiota are associated with a majority of HS lesions. The aim of this study was to characterize the landscape of anaerobic infections in HS using high throughput sequencing...
April 1, 2017: Clinical Infectious Diseases: An Official Publication of the Infectious Diseases Society of America
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