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Microbiology quality

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https://www.readbyqxmd.com/read/29443394/catheter-related-bloodstream-infections-in-adults-receiving-home-parenteral-nutrition-substantial-differences-in-incidence-comparing-a-strict-microbiological-to-a-clinically-based-diagnosis
#1
Siri Tribler, Christopher F Brandt, Mark Hvistendahl, Michael Staun, Per Brøbech, Claus E Moser, Palle B Jeppesen
BACKGROUND: A common complication in patients receiving home parenteral nutrition (HPN) is catheter-related bloodstream infections (CRBSIs). The CRBSI incidence has been advocated as an outcome parameter assessing the quality of care. This study aimed to illustrate how the use of different CRBSI definitions affects the reported incidence. MATERIALS AND METHODS: In an observational study based on the Copenhagen intestinal failure database, all clinically reported CRBSIs from 2002-2013 were compared with data from the affiliated microbiological database according to recommended CRBSI criteria...
February 2018: JPEN. Journal of Parenteral and Enteral Nutrition
https://www.readbyqxmd.com/read/29442048/storage-stability-of-serum-formulations-containing-ofloxacin-for-autologous-serum-eardrop-therapy
#2
N Hashimoto, K Togami, K Endou, H Onodera, H Tanimoto, S Itagaki, S Chono
OBJECTIVE: The storage stability of serum formulations containing ofloxacin for autologous serum eardrop therapy was evaluated for microbiological quality and component stability. METHODS: Sterile serum formulations were prepared by mixing human serum and ofloxacin otic solution (1:1, v/v). To simulate eardrop contamination with external ear surface substances, prepared serum formulations were contaminated with a cotton swab that was rubbed sufficiently on the human external ear...
March 1, 2017: Die Pharmazie
https://www.readbyqxmd.com/read/29439746/antimicrobial-stewardship-program-implementation-of-a-quality-improvement-intervention-using-real-time-feedback-and-an-electronic-order-set-for-the-management-of-staphylococcus-aureus-bacteremia
#3
Rossana Rosa, Bruno Zavala, Natalie Cain, Shweta Anjan, Laura Aragon, Lilian M Abbo
Antimicrobial stewardship programs can optimize the management of Staphylococcus aureus bacteremia by integrating information technology and microbiology laboratory resources. This study describes our experience implementing an intervention consisting of real-time feedback and the use of an electronic order set for the management of S. aureus bacteremia. Infect Control Hosp Epidemiol 2018;1-4.
February 14, 2018: Infection Control and Hospital Epidemiology
https://www.readbyqxmd.com/read/29436857/effect-of-thyme-and-oregano-essential-oils-on-the-shelf-life-of-salmon-and-seaweed-burgers
#4
D Dolea, A Rizo, A Fuentes, J M Barat, I Fernández-Segovia
The effect of different essential oils on the quality and shelf life of fish and seaweed burgers during storage was evaluated. For this purpose, thyme and oregano essential oils were selected at a concentration of 0.05% (v/w). Three types of salmon and seaweed burgers were prepared: without essential oil, burgers with red thyme essential oil (0.05% (v/w)) and burgers with oregano essential oil (0.05% (v/w)), which were vacuum packaged and stored at 4 ℃ for 17 days. Physicochemical and microbiological analyses were carried out periodically throughout storage...
January 1, 2018: Food Science and Technology International, Ciencia y Tecnología de Los Alimentos Internacional
https://www.readbyqxmd.com/read/29433835/towards-automated-detection-semi-quantification-and-identification-of-microbial-growth-in-clinical-bacteriology-a-proof-of-concept
#5
Antony Croxatto, Raphaël Marcelpoil, Cédrick Orny, Didier Morel, Guy Prod'hom, Gilbert Greub
BACKGROUND: Automation in microbiology laboratories impacts management, workflow, productivity and quality. Further improvements will be driven by the development of intelligent image analysis allowing automated detection of microbial growth, release of sterile samples, identification and quantification of bacterial colonies and reading of AST disk diffusion assays. We investigated the potential benefit of intelligent imaging analysis by developing algorithms allowing automated detection, semi-quantification and identification of bacterial colonies...
December 2017: Biomedical Journal
https://www.readbyqxmd.com/read/29433447/comparative-assessment-of-the-quality-of-commercial-black-and-green-tea-using-microbiology-analyses
#6
Federica Carraturo, Olga De Castro, Jacopo Troisi, Adriana De Luca, Armando Masucci, Paola Cennamo, Marco Trifuoggi, Francesco Aliberti, Marco Guida
BACKGROUND: Drinking tea constitutes a tradition which is deeply rooted in the culture of several countries. Moreover, in recent years, tea consumption is growing all over the world. Improper herbal tea storage (long periods, humid environments) represents a relevant health hazard for consumers because of the growth of bacteria and molds. RESULTS: This study analyzed 32 samples of commercially available black and green teas - purchased from southern Italy markets and online-shops - and the monitoring of microbiological quality of the tea bag content was performed...
January 5, 2018: BMC Microbiology
https://www.readbyqxmd.com/read/29433218/application-of-high-throughput-pyrosequencing-in-the-analysis-of-microbiota-of-food-commodities-procured-from-small-and-large-retail-outlets-in-a-u-s-metropolitan-area-a-pilot-study
#7
Daleniece Higgins, Chandan Pal, Irshad M Sulaiman, Chunrong Jia, Tyler Zerwekh, Scot E Dowd, Pratik Banerjee
With the advent of high-throughput sequencing technologies, it is possible to comprehensively analyze the microbial community of foods without culturing them in the laboratory. The estimation of all microbes inhabiting a food commodity (food microbiota) therefore may shed light on the microbial quality and safety of foods. In this study, we utilized high-throughput pyrosequencing of 16S rRNA genes as well as traditional microbiological methods to evaluate the bacterial diversity and the predicted metabolic pathways associated with the bacterial communities of selected foods (romaine lettuce, cabbage, deli meat, and chicken legs, total 200 samples) procured from small and large retail outlets located in Memphis-Shelby County, Tennessee, USA...
March 2018: Food Research International
https://www.readbyqxmd.com/read/29429649/effects-of-ultrasound-energy-density-on-the-non-thermal-pasteurization-of-chocolate-milk-beverage
#8
Sara H M C Monteiro, Eric Keven Silva, Verônica O Alvarenga, Jeremias Moraes, Mônica Q Freitas, Márcia C Silva, Renata S L Raices, Anderson S Sant'Ana, M Angela A Meireles, Adriano G Cruz
This study presents the emerging high-intensity ultrasound (HIUS) processing as a non-thermal alternative to high-temperature short-time pasteurization (HTST). Chocolate milk beverage (CMB) was subjected to different ultrasound energy densities (0.3-3.0 kJ/cm3), as compared to HTST pasteurization (72 °C/15 s) aimed to verify the effect of the HIUS processing on the microbiological and physicochemical characteristics of the beverage. The application of HIUS at an energy density of 3.0 kJ/cm3 was able to reduce 3...
April 2018: Ultrasonics Sonochemistry
https://www.readbyqxmd.com/read/29428064/automatic-hemolysis-identification-on-aligned-dual-lighting-images-of-cultured-blood-agar-plates
#9
Mattia Savardi, Alessandro Ferrari, Alberto Signoroni
BACKGROUND AND OBJECTIVE: The recent introduction of Full Laboratory Automation systems in clinical microbiology opens to the availability of streams of high definition images representing bacteria culturing plates. This creates new opportunities to support diagnostic decisions through image analysis and interpretation solutions, with an expected high impact on the efficiency of the laboratory workflow and related quality implications. Starting from images acquired under different illumination settings (top-light and back-light), the objective of this work is to design and evaluate a method for the detection and classification of diagnostically relevant hemolysis effects associated with specific bacteria growing on blood agar plates...
March 2018: Computer Methods and Programs in Biomedicine
https://www.readbyqxmd.com/read/29426791/collection-transport-and-general-processing-of-clinical-specimens-in-microbiology-laboratory
#10
M Isabel Sánchez-Romero, Juan Manuel García-Lechuz Moya, Juan José González López, Nieves Orta Mira
The interpretation and the accuracy of the microbiological results still depend to a great extent on the quality of the samples and their processing within the Microbiology laboratory. The type of specimen, the appropriate time to obtain the sample, the way of sampling, the storage and transport are critical points in the diagnostic process. The availability of new laboratory techniques for unusual pathogens, makes necessary the review and update of all the steps involved in the processing of the samples. Nowadays, the laboratory automation and the availability of rapid techniques allow the precision and turn-around time necessary to help the clinicians in the decision making...
February 6, 2018: Enfermedades Infecciosas y Microbiología Clínica
https://www.readbyqxmd.com/read/29426419/effects-of-chitosan-based-coatings-enriched-with-procyanidin-by-product-on-quality-of-fresh-blueberries-during-storage
#11
C Mannozzi, U Tylewicz, F Chinnici, L Siroli, P Rocculi, M Dalla Rosa, S Romani
The aim of this work was to evaluate the efficacy of an innovative edible coating, based on chitosan from mushrooms enriched with procyanidins extracted from grape seeds, on fresh blueberry quality maintenance, (weight loss, pH, dry matter, colour, firmness and antioxidant activity) and microbial growth, during 14 days of storage at 4 °C. For weight loss, pH and dry matter no relevant differences were detected among the control and the differently coated samples at each considered storage time. Chitosan and chitosan + procyanidins coatings promoted a slight decrease of luminosity and an increase of blue hue colour of blueberry samples during the whole storage period...
June 15, 2018: Food Chemistry
https://www.readbyqxmd.com/read/29425860/efficacy-of-chlorine-dioxide-on-escherichia-coli-inactivation-during-pilot-scale-fresh-cut-lettuce-processing
#12
J L Banach, L S van Overbeek, M N Nierop Groot, P S van der Zouwen, H J van der Fels-Klerx
Controlling water quality is critical in preventing cross-contamination during fresh produce washing. Process wash water (PWW) quality can be controlled by implementing chemical disinfection strategies. The aim of this study was to evaluate the pilot-scale efficacy of chlorine dioxide (ClO2) during processing on the reduction of Escherichia coli in the PWW and on processed fresh-cut 'Lollo Rossa' lettuce. The objective was to have a residual target concentration of either 5 or 3 mg/L ClO2 in the washing tank (3...
January 31, 2018: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/29425222/the-safety-and-quality-of-pork-and-poultry-meat-imports-for-the-common-european-market-received-at-border-inspection-post-hamburg-harbour-between-2014-and-2015
#13
Wiebke Jansen, Svenja Woudstra, Anja Müller, Nils Grabowski, Gundela Schoo, Bettina Gerulat, Günter Klein, Corinna Kehrenberg
Though imports of products of animal origin into the European Union (EU) have to comply with legal requirements and quality standards of the community, food consignment rejections at external EU borders have been increasing in recent years. This study explored microbiological metrics according to national target and critical values valid for samples at consumer level of 498 fresh poultry meat and 136 fresh pork filets from consignments subjected to physical checks during clearing at the border inspection post Hamburg harbour between January 2014 and December 2015 with ISO standard methods...
2018: PloS One
https://www.readbyqxmd.com/read/29424930/inhaled-mannitol-for-cystic-fibrosis
#14
REVIEW
Sarah J Nevitt, Judith Thornton, Clare S Murray, Tiffany Dwyer
BACKGROUND: Several agents are used to clear secretions from the airways of people with cystic fibrosis. Mannitol increases mucociliary clearance, but its exact mechanism of action is unknown. The dry powder formulation of mannitol may be more convenient and easier to use compared with established agents which require delivery via a nebuliser. Phase III trials of inhaled dry powder mannitol for the treatment of cystic fibrosis have been completed and it is now available in Australia and some countries in Europe...
February 9, 2018: Cochrane Database of Systematic Reviews
https://www.readbyqxmd.com/read/29424725/sodium-hypochlorite-dosage-for-household-and-emergency-water-treatment-updated-recommendations
#15
Natalie Wilhelm, Anya Kaufmann, Elizabeth Blanton, Daniele Lantagne
Household water treatment with chlorine can improve the microbiological quality of household water and reduce diarrheal disease. We conducted laboratory and field studies to inform chlorine dosage recommendations. In the laboratory, reactors of varying turbidity (10-300 NTU) and total organic carbon (0-25 mg/L addition) were created, spiked with Escherichia coli, and dosed with 3.75 mg/L sodium hypochlorite. All reactors had >4 log reduction of E. coli 24 hours after chlorine addition. In the field, we tested 158 sources in 22 countries for chlorine demand...
February 2018: Journal of Water and Health
https://www.readbyqxmd.com/read/29424720/the-effect-of-sodium-thiosulfate-dechlorination-on-fecal-indicator-bacteria-enumeration-laboratory-and-field-data
#16
Anna L Murray, Emily Kumpel, Rachel Peletz, Ranjiv S Khush, Daniele S Lantagne
In microbiological water quality testing, sample dechlorination with sodium thiosulfate is recommended to ensure that results accurately reflect the water quality at sample collection. Nevertheless, monitoring institutions in low-resource settings do not always dechlorinate samples, and there is limited research describing how this practice impacts drinking water quality results. The effect of dechlorination on indicator bacteria counts was evaluated by spiking laboratory water with five Escherichia coli (E...
February 2018: Journal of Water and Health
https://www.readbyqxmd.com/read/29423860/schizosaccharomyces-pombe-biotechnological-applications-in-winemaking
#17
Ángel Benito, Fernando Calderón, Santiago Benito
The traditional way of producing wine is through the use of Saccharomyces cerevisiae in order to convert glucose and fructose into alcohol. In the case of red wines, after this alcoholic fermentation lactic bacteria Oenococus oeni is used to stabilize wine from a microbiological point of view by converting malic acid into lactic acid that it is not a microbiological substract. The yeast species Schizosaccharomyces pombe was traditionally considered spoilage yeast. Nevertheless, during the last decade it started to be used due to its unique malic acid deacidification ability to reduce the harsh acidity of wines from northern Europe, by converting malic acid to ethanol and CO2 without producing lactic acid as lactic bacteria does...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29421093/quality-assessment-of-pollution-indicators-in-marine-water-at-critical-locations-of-the-gulf-of-mannar-biosphere-reserve-tuticorin
#18
Viji Rajendran, Shrinithivihahshini Nirmaladevi D, Balakrishnan Srinivasan, Chithradevi Rengaraj, Sheelamary Mariyaselvam
The present study focused on the shoreline environment of urban and industrial areas, and the aim of this study was to assess the coastal water quality in the Gulf of Mannar Biosphere Reserve. Water samples were collected from five different coastal sites during the premonsoon and monsoon seasons. The samples were analyzed following the standard methods. The results showed that the levels of microbiological indicators in the samples highly exceeded the regional and national standard seawater permissible limits, and environmental parameters such as the total suspended solid and dissolved oxygen were affected significantly (p<0...
January 2018: Marine Pollution Bulletin
https://www.readbyqxmd.com/read/29420818/multi-locus-sequence-typing-provides-epidemiological-insights-for-diseased-sharks-infected-with-fungi-belonging-to-the-fusarium-solani-species-complex
#19
Guillaume Desoubeaux, Anne Debourgogne, Nathan P Wiederhold, Marie Zaffino, Deanna Sutton, Rachel E Burns, Salvatore Frasca, Michael W Hyatt, Carolyn Cray
Fusarium spp. are saprobic moulds that are responsible for severe opportunistic infections in humans and animals. However, we need epidemiological tools to reliably trace the circulation of such fungal strains within medical or veterinary facilities, to recognize environmental contaminations that might lead to infection and to improve our understanding of factors responsible for the onset of outbreaks. In this study, we used molecular genotyping to investigate clustered cases of Fusarium solani species complex (FSSC) infection that occurred in eight Sphyrnidae sharks under managed care at a public aquarium...
October 9, 2017: Medical Mycology: Official Publication of the International Society for Human and Animal Mycology
https://www.readbyqxmd.com/read/29419793/microbiological-values-of-rainwater-harvested-in-adelaide
#20
Chirhakarhula Emmanuel Chubaka, Harriet Whiley, John W Edwards, Kirstin E Ross
In Australia, rainwater is an important source of water for many households. Unlike municipal water, rainwater is often consumed untreated. This study investigated the potential contamination of rainwater by microorganisms. Samples from 53 rainwater tanks across the Adelaide region were collected and tested using Colilert™ IDEXX Quanti-Tray*/2000. Twenty-eight out of the 53 tanks (53%) contained Escherichia coli. Samples collected from ten tanks contained E. coli at concentrations exceeding the limit of 150 MPN/100 mL for recreational water quality...
February 8, 2018: Pathogens
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