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Microbiology quality

Jordana E Hoppe, Brandie D Wagner, Frank J Accurso, Edith T Zemanick, Scott D Sagel
BACKGROUND: Pulmonary exacerbations (PEx) in children with cystic fibrosis (CF) are frequently treated in the outpatient setting with oral antibiotics. However, little is known about the characteristics of PEx managed on an outpatient basis and the effectiveness of oral antibiotic therapy. We sought to prospectively evaluate clinical and laboratory changes associated with oral antibiotic treatment for PEx. METHODS: Children with CF between 8 and 18 years of age prescribed two weeks of oral antibiotics for a PEx were eligible to enroll...
June 16, 2018: Journal of Cystic Fibrosis: Official Journal of the European Cystic Fibrosis Society
Pi-Yu Chen, Chi-Ying Hsieh, Chao-Jen Shih, Yuan-Jing Lin, Chia-Wen Tsao, Yu-Liang Yang
Application of matrix-assisted laser desorption/ionization imaging mass spectrometry to microbiology and natural product research has opened the door to the exploration of microbial interactions and the consequent discovery of new natural products and their functions in the interactions. However, several drawbacks of matrix-assisted laser desorption/ionization imaging mass spectrometry have limited its application especially to complicated and uneven microbial samples. Here, we applied nanostructured silicon as a substrate for surface-assisted laser desorption/ionization mass spectrometry for microbial imaging mass spectrometry to explore fungal metabolic interactions...
June 19, 2018: Journal of Natural Products
Vishal Diwan, Nada Hanna, Manju Purohit, Salesh Chandran, Emilia Riggi, Vivek Parashar, Ashok J Tamhankar, Cecilia Stålsby Lundborg
OBJECTIVES: To characterize the seasonal variation, over one year, in water-quality, antibiotic residue levels, antibiotic resistance genes and antibiotic resistance in Escherichia coli isolates from water and sediment of the Kshipra River in Central India. METHODS: Water and sediment samples were collected from seven selected points from the Kshipra River in the Indian city of Ujjain in the summer, rainy season, autumn and winter seasons in 2014. Water quality parameters (physical, chemical and microbiological) were analyzed using standard methods...
June 17, 2018: International Journal of Environmental Research and Public Health
Johan Bengtsson-Palme, Rodney T Richardson, Marco Meola, Christian Wurzbacher, Émilie D Tremblay, Kaisa Thorell, Kärt Kanger, K Martin Eriksson, Guillaume J Bilodeau, Reed M Johnson, Martin Hartmann, R Henrik Nilsson
Motivation: Correct taxonomic identification of DNA sequences is central to studies of biodiversity using both shotgun metagenomic and metabarcoding approaches. However, no genetic marker gives sufficient performance across all the biological kingdoms, hampering studies of taxonomic diversity in many groups of organisms. This has led to the adoption of a range of genetic markers for DNA metabarcoding. While many taxonomic classification software tools can be re-trained on these genetic markers, they are often designed with assumptions that impair their utility on genes other than the SSU and LSU rRNA...
June 15, 2018: Bioinformatics
Anil Reddy Konduru, Balasaheb R Yelikar, K V Sathyashree, Ankur Kumar
Background: Open source technologies and mobile innovations have radically changed the way people interact with technology. These innovations and advancements have been used across various disciplines and already have a significant impact. Microscopy, with focus on visually appealing contrasting colors for better appreciation of morphology, forms the core of the disciplines such as Pathology, microbiology, and anatomy. Here, learning happens with the aid of multi-head microscopes and digital camera systems for teaching larger groups and in organizing interactive sessions for students or faculty of other departments...
2018: Journal of Pathology Informatics
Alexander A Alles, Martin Wiedmann, Nicole H Martin
Microbial spoilage of pasteurized fluid milk is typically due to either (1) postpasteurization contamination (PPC) with psychrotolerant gram-negative bacteria (predominantly Pseudomonas) or (2) growth of psychrotolerant sporeformers (e.g., Paenibacillus) that have the ability to survive pasteurization when present as spores in raw milk, and to subsequently grow at refrigeration temperatures. While fluid milk quality has improved over the last several decades, continued reduction of PPC is hampered by the lack of rapid, sensitive, and specific methods that allow for detection of PPC in fluid milk, with fluid milk processors still often using time-consuming methods (e...
June 13, 2018: Journal of Dairy Science
Michael A Noble, Robert Rennie
BACKGROUND: Reporting on the presence of antimicrobial resistance is of considerable concern both for individual patient care and for understanding the underlying health status within the community at large. Antimicrobial resistance is solely dependent upon clinical laboratory detection and thus can be impacted upon by the quality and competence of medical laboratories. Proficiency testing or external quality assessment (PT/EQA) is the international standard for the direct measurement of medical laboratory performance on critical testing...
June 15, 2018: Diagnosis
Ednilse Leme, Ericka P Silva, Paula S Rodrigues, Igor R Silva, Maria F M Martins, Eduardo F Bondan, Maria M Bernardi, Thiago B Kirsten
The Billings reservoir is the largest water-storage facility in the São Paulo Metropolitan Region, with only a small part of the reservoir used for water supply. Recently, the São Paulo Metropolitan Region has experienced the greatest water collapse ever recorded. Thus, the intensification of use of the Billings reservoir should be considered. The objective of this study was to evaluate the quality of the water from different areas of the Billings reservoir related to human consumption (water supply and fishing): Rio Pequeno, Rio Grande, and Bororé rivers...
June 11, 2018: Ecotoxicology and Environmental Safety
Khadijeh Jafari, Ali Akbar Mohammadi, Zahra Heidari, Farzaneh Baghal Asghari, Majid Radfard, Mahmood Yousefi, Mahmoud Shams
A lack of access to safe drinking water can lead to adverse health effects such as infection, disease, and undesirable aesthetic problems. The current study focused on the investigation of groundwater quality in Tiran's villages (Isfahan province, Iran). To determine essential microbiological quality, water samples were collected from 46 randomly-selected water wells during a one-year period. The parameters of pH and chlorine were measured on-site. Turbidity was measured at 420 nm using a DR5000 spectrophotometer...
June 2018: Data in Brief
Estelle Baurès, Olivier Blanchard, Fabien Mercier, Emilie Surget, Pierre le Cann, Alexandre Rivier, Jean-Pierre Gangneux, Arnaud Florentin
In addition to being influenced by the environment, the indoor air pollution in hospitals may be associated with specific compounds emitted from various products used, health care activities and building materials. This study has enabled assessment of the chemical and microbiological concentrations of indoor air in two French hospitals. Based on an integrated approach, the methodology defined aims to measure concentrations of a wide range of chemical compounds (>50 volatile and semi-volatile organic compounds), particle concentrations (PM10 and PM2...
June 9, 2018: Science of the Total Environment
Danice Guzmán, Justin Stoler
Packaged water, particularly bagged sachet water, has become an important drinking water source in West Africa as local governments struggle to provide safe drinking water supplies. In Ghana, sachet water has become an important primary water source in urban centers, and a growing literature has explored various dimensions of this industry, including product quality. There is very little data on sachet water quality outside of large urban centers, where smaller markets often mean less producer competition and less government regulation...
June 11, 2018: American Journal of Tropical Medicine and Hygiene
Lambert M Felix, Seamus Grundy, Stephen J Milan, Ross Armstrong, Haley Harrison, Dave Lynes, Sally Spencer
BACKGROUND: Bronchiectasis is a chronic respiratory disease characterised by abnormal and irreversible dilatation of the smaller airways and associated with a mortality rate greater than twice that of the general population. Antibiotics serve as front-line therapy for managing bacterial load, but their use is weighed against the development of antibiotic resistance. Dual antibiotic therapy has the potential to suppress infection from multiple strains of bacteria, leading to more successful treatment of exacerbations, reduced symptoms, and improved quality of life...
June 11, 2018: Cochrane Database of Systematic Reviews
Godfred O Boateng, Shalean M Collins, Patrick Mbullo, Pauline Wekesa, Maricianah Onono, Torsten B Neilands, Sera L Young
Our ability to measure household-level food insecurity has revealed its critical role in a range of physical, psychosocial, and health outcomes. Currently, there is no analogous, standardized instrument for quantifying household-level water insecurity, which prevents us from understanding both its prevalence and consequences. Therefore, our objectives were to develop and validate a household water insecurity scale appropriate for use in our cohort in western Kenya. We used a range of qualitative techniques to develop a preliminary set of 29 household water insecurity questions and administered those questions at 15 and 18 months postpartum, concurrent with a suite of other survey modules...
2018: PloS One
R J Gruninger, R J Forster, T A McAllister
Metatranscriptomics can be used to examine both the composition of a microbial community as well as its metabolic activity under a particular set of conditions and complement metagenomic studies. The availability of low-cost, high-throughput next-generation sequencing has led to a rapid increase in the number of metatranscriptomic studies being undertaken. One of the primary difficulties when conducting transcriptomics is the ability to isolate high-quality RNA from samples of interest. The application of metatranscriptomics in rumen microbiology is still relatively novel but there is a significant push toward applying this technology in this field...
2018: Methods in Molecular Biology
Mahmood Yousefi, Hossein Najafi Saleh, Mehdi Yaseri, Amir Hossein Mahvi, Hamed Soleimani, Zhyar Saeedi, Sara Zohdi, Ali Akbar Mohammadi
In this research, the villages with water supply systems under the supervision of the Water and Wastewater Company in Poldasht County, Iran in 2015 was studied. 648 samples were taken from 57 villages during 12month period to test for microbial quality according to the latest guidelines of WHO. Fecal coliform, coliform, turbidity, pH and free residual chlorine were analyzed. Also we used linear Regression statistical analysis for collected data. Result of Data showed that 13.6% of the villages under study had contaminated water resources...
April 2018: Data in Brief
Frederick Asare, Lobina G Palamuleni, Tabukeli Ruhiiga
In the semi-arid environments of the North West province of South Africa the amount, timing, and distribution of rainfall is irregular, while water accessibility is a key factor in production. In line with this, a study was conducted to assess the impact of land use change on water quality and water depth within the sub-catchment areas of ephemeral ponds. To determine land use dynamics, 2004 and 2013 Landsat images were classified using maximum likelihood algorithm. Pond water quality was analysed for physical, chemical, and microbiological parameters using standard the American Public Health Association (APHA) methods...
June 5, 2018: International Journal of Environmental Research and Public Health
Ana Rodríguez, Mercedes Tajuelo, Diana Rodríguez, Susana Seseña, Patricia Ruiz, Mª Llanos Palop
The aim of this study was to evaluate the air quality of an indoor swimming pool, analysing diurnal and seasonal variations in microbiological counts and chemical parameters. The results indicated that yeast and bacteria counts, as well as carbon dioxide (CO2 ), nitrogen oxides (NOx ) and O3 concentrations, showed significant diurnal difference. On the other hand, temperature, relative humidity (R.H.), yeast counts and concentrations of CO2 , particles, O3 , toluene, and benzene showed seasonal differences...
June 5, 2018: Indoor Air
Alice Melocchi, Federico Parietti, Simone Maccagnan, Marco Aldo Ortenzi, Stefano Antenucci, Francesco Briatico-Vangosa, Alessandra Maroni, Andrea Gazzaniga, Lucia Zema
Following recent advances in nutrigenomics and nutrigenetics, as well as in view of the increasing use of nutraceuticals in combination with drug treatments, considerable attention is being directed to the composition, bioefficacy, and release performance of dietary supplements. Moreover, the interest in the possibility of having such products tailored to meet specific needs is fast growing among costumers. To fulfill these emerging market trends, 3D-printed capsular devices originally intended for conveyance and administration of drugs were proposed for delivery of dietary supplements...
June 5, 2018: AAPS PharmSciTech
Diana Escuder-Vieco, Irene Espinosa-Martos, Juan M Rodríguez, Nieves Corzo, Antonia Montilla, Pablo Siegfried, Carmen R Pallás-Alonso, Leónides Fernández
Donor milk is the best alternative for the feeding of preterm newborns when mother's own milk is unavailable. For safety reasons, it is usually pasteurized by the Holder method (62.5°C for 30 min). Holder pasteurization results in a microbiological safe product but impairs the activity of many biologically active compounds such as immunoglobulins, enzymes, cytokines, growth factors, hormones or oxidative stress markers. High-temperature short-time (HTST) pasteurization has been proposed as an alternative for a better preservation of some of the biological components of human milk although, at present, there is no equipment available to perform this treatment under the current conditions of a human milk bank...
2018: Frontiers in Microbiology
Paola Lavermicocca, Luisa Angiolillo, Stella L Lonigro, Francesca Valerio, Antonio Bevilacqua, Marianne Perricone, Matteo A Del Nobile, Maria R Corbo, Amalia Conte
This paper proposes bio-preservation as a tool to assure quality and safety of Spanish-style table olives cv. Bella di Cerignola. Lactobacillus plantarum 5BG was inoculated in ready to sell olives packaged in an industrial plant by using a half-volume brine (4% NaCl; 2% sucrose). The samples were stored at 4°C. The survival of the inoculated strain, the microbiological quality, the sensory scores and the survival of a strain of Listeria monocytogenes inoculated in brines were evaluated. The persistence of the Lb...
2018: Frontiers in Microbiology
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