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https://www.readbyqxmd.com/read/29050406/microbiological-quality-assessment-and-validation-of-antimicrobials-against-unstressed-or-cold-stress-adapted-salmonella-and-surrogate-enterococcus-faecium-on-broiler-carcasses-and-wings
#1
Lacey Lemonakis, KaWang Li, Jeremy M Adler, Cangliang Shen
This study aims to evaluate the microbiological quality and efficacy of antimicrobials to inactivate unstressed or cold-stress adapted Salmonella and Enterococcus on broiler carcasses and wings processed at a small USDA-inspected slaughter facility in West Virginia. The first part of the study included 42 carcasses that were pre- and secondarily-enriched in bacterial media followed by streak-plating onto XLT-4 and HardyCHROM™-agar Salmonella and confirmation using an API20E-kit. The aerobic plate counts (APC), Escherichia coli (ECC), total coliforms (TCC), and yeast/molds were analyzed on petri-films...
September 1, 2017: Poultry Science
https://www.readbyqxmd.com/read/29040084/quantification-and-identification-of-aerobic-bacteria-in-holy-water-samples-from-a-german-environment
#2
Christoph König, Stephanie Tauchnitz, Heike Kunzelmann, Christian Horn, Frithjof Blessing, Matthias Kohl, Markus Egert
Despite its frequent use in many religious institutions, the microbiological quality of holy water is clearly underinvestigated. We analyzed the microbial load of 54 holy water samples, repeatedly taken in five Roman Catholic churches in the greater area of Villingen-Schwenningen, Germany, by means of aerobic colony counting and Matrix-Assisted Laser Desorption/Ionization (MALDI) Biotyping of representative isolates. Over all samples, colony counting indicated an average aerobic microbial load of 5.85 ± 3...
October 2017: Journal of Water and Health
https://www.readbyqxmd.com/read/29039846/gut-bacterial-communities-of-diarrheic-patients-with-indications-of-clostridioides-difficile-infection
#3
Dominik Schneider, Andrea Thürmer, Kathleen Gollnow, Raimond Lugert, Katrin Gunka, Uwe Groß, Rolf Daniel
We present bacterial 16S rRNA gene datasets derived from stool samples of 44 patients with diarrhea indicative of a Clostridioides difficile infection. For 20 of these patients, C. difficile infection was confirmed by clinical evidence. Stool samples from patients originating from Germany, Ghana, and Indonesia were taken and subjected to DNA isolation. DNA isolations of stool samples from 35 asymptomatic control individuals were performed. The bacterial community structure was assessed by 16S rRNA gene analysis (V3-V4 region)...
October 17, 2017: Scientific Data
https://www.readbyqxmd.com/read/29034325/collection-of-viable-aerosolized-influenza-virus-and-other-respiratory-viruses-in-a-student-health-care-center-through-water-based-condensation-growth
#4
Maohua Pan, Tania S Bonny, Julia Loeb, Xiao Jiang, John A Lednicky, Arantzazu Eiguren-Fernandez, Susanne Hering, Z Hugh Fan, Chang-Yu Wu
The dynamics and significance of aerosol transmission of respiratory viruses are still controversial, for the major reasons that virus aerosols are inefficiently collected by commonly used air samplers and that the collected viruses are inactivated by the collection method. Without knowledge of virus viability, infection risk analyses lack accuracy. This pilot study was performed to (i) determine whether infectious (viable) respiratory viruses in aerosols could be collected from air in a real world environment by the viable virus aerosol sampler (VIVAS), (ii) compare and contrast the efficacy of the standard bioaerosol sampler, the BioSampler, with that of the VIVAS for the collection of airborne viruses in a real world environment, and (iii) gain insights for the use of the VIVAS for respiratory virus sampling...
September 2017: MSphere
https://www.readbyqxmd.com/read/29032906/live-dead-discrimination-analysis-qpcr-assessment-for-opportunistic-pathogens-and-population-analysis-at-ozone-wastewater-treatment-plants
#5
Thomas Jäger, Johannes Alexander, Silke Kirchen, Andreas Dötsch, Arne Wieland, Christian Hiller, Thomas Schwartz
In respect to direct and indirect water reuse, the microbiological quality of treated wastewater is highly important. Conventional wastewater treatment plants are normally not equipped with advanced technologies for the elimination of bacteria. Molecular biology analyses were combined with live-dead discrimination analysis of wastewater population using Propidium monoazide (PMA) to study population shifts during ozonation (1 g ozone/g DOC) at a municipal wastewater treatment plant. Escherichia coli, enterococci, and Pseudomonas aeruginosa were quantified by polymerase chain reaction (qPCR) and the whole wastewater population was analyzed by metagenomic sequencing...
October 9, 2017: Environmental Pollution
https://www.readbyqxmd.com/read/29031605/a-waterborne-outbreak-of-multiple-diarrhoeagenic-escherichia-coli-infections-associated-with-drinking-water-at-a-school-camp
#6
Jungsun Park, Jin Seok Kim, Soojin Kim, Eunkyung Shin, Kyung-Hwan Oh, Yonghoon Kim, Cheon Hyeon Kim, Min Ah Hwang, Chan Mun Jin, Kyoungin Na, Jin Lee, Enhi Cho, Byung-Hak Kang, Hyo-Sun Kwak, Won Keun Seong, Junyoung Kim
BACKGROUND: In June 2015, a local public health laboratory was notified that students had developed gastroenteritis symptoms after participating in a camp. An outbreak investigation was conducted to determine the extent and cause of the outbreak. METHOD: We conducted a retrospective cohort study to determine the correlations between the illness and specific exposures at the school camp. All attendees were interviewed with a standard questionnaire that addressed clinical symptoms, food consumption and environmental exposures...
October 11, 2017: International Journal of Infectious Diseases: IJID
https://www.readbyqxmd.com/read/29031434/validation-of-an-electronic-tool-for-flagging-surgical-site-infections-based-on-clinical-practice-patterns-for-triaging-surveillance-operational-successes-and-barriers
#7
T Pindyck, K Gupta, J Strymish, K M Itani, M E Carter, Y Suo, M T Bessesen, J Topkoff, A S Steele, A E Barón, A V Gundlapalli, W Branch-Elliman
BACKGROUND: Surveillance is an effective strategy for reducing surgical site infections (SSIs); however, current identification methods are resource-intensive. Therefore, we sought to validate an electronic SSI triaging tool for detection of probable infections and identify operational barriers and challenges. METHODS: A retrospective cohort study was conducted among all Veterans Affairs Surgical Quality Improvement Program (VASQIP)-reviewed surgeries at 2 Veterans Affairs medical centers from October 1, 2011-September 30, 2014...
October 11, 2017: American Journal of Infection Control
https://www.readbyqxmd.com/read/29030529/microbiological-test-data-assuring-data-integrity
#8
Edward Charles Tidswell, Tim Sandle
Marketed drugs and devices possess specifications including critical microbiological quality attributes purposed to assure efficacy and patient safety. These attributes are legislated requirements intended to protect the recipient patient. Sampling, microbiological testing, interpretation of data for final products, raw materials and intermediates all contribute to a cohesive assessment in the assurance of finished product quality. Traditional culture-based microbiological methods possess inherent and unavoidable variability, recognized by the compendia and which might lead to erroneous conclusion pertaining to product quality...
October 12, 2017: PDA Journal of Pharmaceutical Science and Technology
https://www.readbyqxmd.com/read/29030528/real-time-pcr-detection-of-burkholderia-cepacia-in-pharmaceutical-products-contaminated-with-low-levels-of-bacterial-contamination
#9
Luis Jimenez, Theranda Jashari, Jenifer Vasquez, Stephanie Zapata, Joy Bochis, Margarita Kulko, Victoria Ellman, Matthew Gardner, Tina Choe
A Real-Time (RT) PCR assay was developed to detect Burkholderia cepacia in pharmaceutical products contaminated with low levels of bacterial contamination. Different pharmaceutical suspensions were artificially contaminated with B. cepacia, Escherichia coli, Staphylococcus aureus, and Bacillus megaterium. After a 24-hour incubation in TSB with Tween 20, samples were streaked on mannitol salt, phenyl ethyl alcohol, eosin methylene blue, MacConkey, and pseudomonas isolation agar. Microbial DNA was extracted from each sample by using a Tris-EDTA, proteinase K, Tween 20 buffer...
October 12, 2017: PDA Journal of Pharmaceutical Science and Technology
https://www.readbyqxmd.com/read/29028469/the-importance-of-clinicians-and-community-members-receiving-timely-and-accurate-information-about-waterborne-hazards
#10
Steven S Coughlin, Osman Yousufzai
It is important for clinicians and community members to receive up-to-date information about the microbiological and elemental composition of local water supplies. Clinicians play an important role in helping their patients to interpret water quality data and understand the potential impact of water quality on their health. Expanding the medical school curriculum to include environmental health, public health, and health disparities-including disparities related to environmental quality and waterborne hazards-is key to clinicians' fulfilling this role...
October 1, 2017: AMA Journal of Ethics
https://www.readbyqxmd.com/read/29026631/salmonella-typhimurium-gastroenteritis-leading-to-chronic-prosthetic-vascular-graft-infection
#11
Milo Cullinan, Michael Clarke, Tim Dallman, Steven Peart, Deborah Wilson, Daniel Weiand
Introduction. It is estimated up to 6 % of prosthetic vascular grafts become infected. Staphylococcus aureus is predominant in early infection and coagulase-negative staphylococci are predominant in late infections. Enterobacteriaceae cause 14-40 % of prosthetic vascular graft infections. This is, to our knowledge the first reported case of Salmonella gastroenteritis causing chronic prosthetic vascular graft infection (PVGI). Case presentation. A 57 years old lady presented with signs and symptoms of prosthetic vascular graft infection...
August 2017: JMM Case Reports
https://www.readbyqxmd.com/read/29026355/colistin-and-polymyxin-b-minimal-inhibitory-concentrations-determined-by-etest-found-unreliable-for-gram-negative-bacilli
#12
Shelby Simar, Diane Sibley, Deborah Ashcraft, George Pankey
BACKGROUND: A reliable method of polymyxin B and E (colistin) susceptibility testing remains elusive. These drugs diffuse poorly into agar, creating potentially inaccurate Etest and disk diffusion results, and testing by these methods is not recommended. Broth microdilution is the reference testing method, although it can be sometimes difficult to interpret. Currently, when a colistin susceptibility test is ordered for a patient in the Ochsner Health System, our diagnostic microbiology laboratory performs the Etest...
2017: Ochsner Journal
https://www.readbyqxmd.com/read/29023716/production-of-cornstarch-granules-enriched-with-quercetin-liposomes-by-aggregation-of-particulate-binary-mixtures-using-high-shear-process
#13
Taíse Toniazzo, Helena Galeskas, Gustavo C Dacanal, Samantha C Pinho
Liposomes are colloidal structures capable of encapsulating, protecting, and releasing hydrophobic bioctives, as flavonoids. Quercetin is a flavonoid with high antioxidant activity that provides benefits to health. The wet-agglomeration processes in high-shear equipment are useful to produce granules from binary mixtures, obtaining a powder with homogeneous composition and without segregation or elutriation of fine particles. In this study, the binary mixtures containing microparticles of native cornstarch and nanoparticles of quercetin liposomes were aggregated in high-shear batches, using maltodextrin solution as binder agent...
October 12, 2017: Journal of Food Science
https://www.readbyqxmd.com/read/29022243/pyrosequencing-analysis-of-source-water-switch-and-sulfate-induced-bacterial-community-transformation-in-simulated-drinking-water-distribution-pipes
#14
Fan Yang, Baoyou Shi, Weiyu Zhang, Jing Cui, Jianbo Guo, Dongsheng Wang, Nan Wu, Xinyuan Liu
Inter-basin water transfer and source water switching will be increasingly launched due to significant population increase and the shortage of the local water resources in cities around the world. Source water switch may cause physiochemical and microbiological de-stabilization of pipe material, biofilms, and loose deposits in drinking water distribution system (DWDS). Great sulfate alteration during source water switch had been deemed as the main cause of a red water case that occurred in a northern China city...
October 11, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/29021895/readiness-for-antimicrobial-resistance-amr-surveillance-in-pakistan-a-model-for-laboratory-strengthening
#15
Dania Khalid Saeed, Rumina Hasan, Mahwish Naim, Afia Zafar, Erum Khan, Kausar Jabeen, Seema Irfan, Imran Ahmed, Mohammad Zeeshan, Zabin Wajidali, Joveria Farooqi, Sadia Shakoor, Abdul Chagla, Jason Rao
BACKGROUND: Limited capacity of laboratories for antimicrobial susceptibility testing (AST) presents a critical diagnostic bottleneck in resource limited countries. This paper aims to identify such gaps and to explore whether laboratory networks could contribute towards improving AST in low resource settings. METHODS: A self-assessment tool to assess antimicrobial susceptibility testing capacity was administered as a pre-workshop activity to participants from 30 microbiology laboratories in 3 cities in Pakistan...
2017: Antimicrobial Resistance and Infection Control
https://www.readbyqxmd.com/read/29021151/rapid-nanopore-sequencing-of-plasmids-and-resistance-gene-detection-in-clinical-isolates
#16
Jamie K Lemon, Pavel P Khil, Karen M Frank, John P Dekker
Recent advances in nanopore sequencing technology have led to a substantial increase in throughput and sequence quality. Together, these improvements may permit real-time benchtop genomic sequencing and antimicrobial resistance gene detection in clinical isolates. Here, we evaluated workflows and turnaround times for a benchtop long-read sequencing approach in the clinical microbiology laboratory using the Oxford Nanopore Technologies MinION sequencer. We performed genomic and plasmid sequencing of three clinical isolates with both MinION and Illumina MiSeq, using different library preparation methods (2D and rapid 1D) with the goal of antimicrobial resistance gene detection...
October 11, 2017: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/29019097/efficacy-of-conventional-and-liposomal-povidone-iodine-in-infected-mesh-skin-grafts-an-exploratory-study
#17
Peter M Vogt, Joerg Hauser, Stefan Mueller, Bjoern Bosse, Michael Hopp
INTRODUCTION: Infection is a major threat to wound healing and a leading cause of graft loss in patients undergoing meshed skin grafts (MSGs). Therefore, topical antisepsis is important in the overall treatment scheme. METHODS: An exploratory satellite group of 14 patients with infected MSGs were enrolled as part of a prospective, randomized, controlled, parallel-group, open-label, pilot Phase II study that investigated the efficacy and tolerability of 3% liposomal povidone-iodine hydrogel (PVP-ILH, Repithel(®); RepiGel(®)) versus chlorhexidine gauze in non-infected MSGs...
October 10, 2017: Infectious Diseases and Therapy
https://www.readbyqxmd.com/read/29016644/accuracy-of-xpert-clostridium-difficile-assay-for-the-diagnosis-of-clostridium-difficile-infection-a-meta-analysis
#18
Yuanyuan Bai, Xiaorong Sun, Yan Jin, Yueling Wang, Juan Li
BACKGROUND: There is an urgent need for rapid and accurate microbiological diagnostic assay for detection of Clostridium difficile infection (CDI). We assessed the diagnostic accuracy of the Xpert Clostridium difficile assay (Xpert CD) for the diagnosis of CDI. METHODS: We searched PubMed, EMBASE, and Cochrane Library databases to identify studies according to predetermined criteria. STATA 13.0 software was used to analyze the tests for sensitivity, specificity, positive likelihood ratio, negative likelihood ratio, diagnostic odds ratio, and area under the summary receiver operating characteristic curves (AUC)...
2017: PloS One
https://www.readbyqxmd.com/read/29016318/effects-of-boiling-drinking-water-on-diarrhea-and-pathogen-specific-infections-in-low-and-middle-income-countries-a-systematic-review-and-meta-analysis
#19
Alasdair Cohen, John M Colford
Globally, approximately 2 billion people lack microbiologically safe drinking water. Boiling is the most prevalent household water treatment method, yet evidence of its health impact is limited. To conduct this systematic review, we searched four online databases with no limitations on language or publication date. Studies were eligible if health outcomes were measured for participants who reported consuming boiled and untreated water. We used reported and calculated odds ratios (ORs) and random-effects meta-analysis to estimate pathogen-specific and pooled effects by organism group and nonspecific diarrhea...
September 5, 2017: American Journal of Tropical Medicine and Hygiene
https://www.readbyqxmd.com/read/28991989/impact-of-roadway-stormwater-runoff-on-microbial-contamination-in-the-receiving-stream
#20
Kristen N Wyckoff, Si Chen, Andrew J Steinman, Qiang He
Stormwater runoff from roadways has increasingly become a regulatory concern for water pollution control. Recent work has suggested roadway stormwater runoff as a potential source of microbial pollutants. The objective of this study was to determine the impact of roadway runoff on the microbiological quality of receiving streams. Microbiological quality of roadway stormwater runoff and the receiving stream was monitored during storm events with both cultivation-dependent fecal bacteria enumeration and cultivation-independent high-throughput sequencing techniques...
September 2017: Journal of Environmental Quality
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