keyword
MENU ▼
Read by QxMD icon Read
search

Viruses in soil

keyword
https://www.readbyqxmd.com/read/29453056/biological-properties-of-beet-soil-borne-mosaic-virus-and-beet-necrotic-yellow-vein-virus-cdna-clones-produced-by-isothermal-in-vitro-recombination-insights-for-reassortant-appearance
#1
Marlene Laufer, Hamza Mohammad, Edgar Maiss, Katja Richert-Pöggeler, Mattia Dall'Ara, Claudio Ratti, David Gilmer, Sebastian Liebe, Mark Varrelmann
Two members of the Benyviridae family and genus Benyvirus, Beet soil-borne mosaic virus (BSBMV) and Beet necrotic yellow vein virus (BNYVV), possess identical genome organization, host range and high sequence similarity; they infect Beta vulgaris with variable symptom expression. In the US, mixed infections are described with limited information about viral interactions. Vectors suitable for agroinoculation of all genome components of both viruses were constructed by isothermal in vitro recombination. All 35S promoter-driven cDNA clones allowed production of recombinant viruses competent for Nicotiana benthamiana and Beta macrocarpa systemic infection and Polymyxa betae transmission and were compared to available BNYVV B-type clone...
February 13, 2018: Virology
https://www.readbyqxmd.com/read/29431919/host-induced-gene-silencing-of-a-regulator-of-g-protein-signaling-gene-vdrgs1-confers-resistance-to-verticillium-wilt-in-cotton
#2
Jun Xu, Xinyu Wang, Yongqing Li, Jianguo Zeng, Guilin Wang, Chaoyang Deng, Wangzhen Guo
Verticillium wilt (VW), caused by soil-borne fungi of the genus Verticillium, is a serious disease affecting a wide range of plants and leading to a constant and major challenge to agriculture worldwide. Cotton (Gossypium hirsutum) is the world's most important natural textile fiber and oil crop. VW of cotton is a highly devastating vascular disease, however, few resistant germplasms have been reported in cotton. An increasing number of studies have shown that RNA interference (RNAi) based host-induced gene silencing (HIGS) is an effective strategy for improving plant resistance to pathogens by silencing genes essential for the pathogenicity of these pathogens...
February 12, 2018: Plant Biotechnology Journal
https://www.readbyqxmd.com/read/29397509/essential-oils-from-foeniculum-vulgare-miller-as-a-safe-environmental-insecticide-against-the-aphid-myzus-persicae-sulzer
#3
Roman Pavela
Aphids are an important agricultural pest that not only damage plants by suction, but can also transmit a number of economically important plant viruses. Protection against aphids is based on the use of synthetic insecticides. However, these products can be dangerous for non-target organisms. Therefore, it is important to develop new, environmentally safe plant protection methods.In this study, we have tested an essential oil (EO) obtained from Foeniculum vulgare for the mortality of Myzus persicae, an important polyphagous pest, its natural predator Harmonia axyridis, and Eisenia fetida as a representative of soil organisms...
February 3, 2018: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/29379178/deposition-rates-of-viruses-and-bacteria-above-the-atmospheric-boundary-layer
#4
Isabel Reche, Gaetano D'Orta, Natalie Mladenov, Danielle M Winget, Curtis A Suttle
Aerosolization of soil-dust and organic aggregates in sea spray facilitates the long-range transport of bacteria, and likely viruses across the free atmosphere. Although long-distance transport occurs, there are many uncertainties associated with their deposition rates. Here, we demonstrate that even in pristine environments, above the atmospheric boundary layer, the downward flux of viruses ranged from 0.26 × 109 to >7 × 109 m-2 per day. These deposition rates were 9-461 times greater than the rates for bacteria, which ranged from 0...
January 29, 2018: ISME Journal
https://www.readbyqxmd.com/read/29362410/new-live-screening-of-plant-nematode-interactions-in-the-rhizosphere
#5
Felicity E O'Callaghan, Roberto A Braga, Roy Neilson, Stuart A MacFarlane, Lionel X Dupuy
Free living nematodes (FLN) are microscopic worms found in all soils. While many FLN species are beneficial to crops, some species cause significant damage by feeding on roots and vectoring viruses. With the planned legislative removal of traditionally used chemical treatments, identification of new ways to manage FLN populations has become a high priority. For this, more powerful screening systems are required to rapidly assess threats to crops and identify treatments efficiently. Here, we have developed new live assays for testing nematode responses to treatment by combining transparent soil microcosms, a new light sheet imaging technique termed Biospeckle Selective Plane Illumination Microscopy (BSPIM) for fast nematode detection, and Confocal Laser Scanning Microscopy for high resolution imaging...
January 23, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29348657/an-ethylene-response-factor-mxerf4-functions-as-a-repressor-of-fe-acquisition-in-malus-xiaojinensis
#6
Wei Liu, Ting Wu, Qiwei Li, Xinzhong Zhang, Xuefeng Xu, Tianhong Li, Zhenhai Han, Yi Wang
Iron (Fe) is an essential element for plants; however, its availability is limited as it forms insoluble complexes in the soil. Consequently, plants have developed mechanisms to adapt to low Fe conditions. We demonstrate that ethylene is involved in Fe deficiency-induced physiological responses in Malus xiaojinensis, and describe the identification of MxERF4 as a protein-protein interaction partner with the MxFIT transcription factor, which is involved in the iron deficiency response. Furthermore, we demonstrate that MxERF4 acts as an MxFIT interaction partner to suppresses the expression of the Fe transporter MxIRT1, by binding directly to its promoter, requiring the EAR motif of the MxERF4 protein...
January 18, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29322917/identification-of-natural-antimicrobial-peptides-from-bacteria-through-metagenomic-and-metatranscriptomic-analysis-of-high-throughput-transcriptome-data-of-taiwanese-oolong-teas
#7
Kai-Yao Huang, Tzu-Hao Chang, Jhih-Hua Jhong, Yu-Hsiang Chi, Wen-Chi Li, Chien-Lung Chan, K Robert Lai, Tzong-Yi Lee
BACKGROUND: Anti-microbial peptides (AMPs), naturally encoded by genes and generally containing 12-100 amino acids, are crucial components of the innate immune system and can protect the host from various pathogenic bacteria and viruses. In recent years, the widespread use of antibiotics has resulted in the rapid growth of antibiotic-resistant microorganisms that often induce critical infection and pathogenesis. Recently, the advent of high-throughput technologies has led molecular biology into a data surge in both the amount and scope of data...
December 21, 2017: BMC Systems Biology
https://www.readbyqxmd.com/read/29320442/optimization-of-fermentation-conditions-and-bench-scale-for-improvement-of-a-novel-glycoprotein-gp-1-production-by-streptomyces-kanasenisi-zx01
#8
Yong Zhou, Xin Zhou, Dai-Lin Yu, Bu Sang, Jun-Tao Feng, Li-Rong Han, Xing Zhang
GP-1 is a novel glycoprotein produced by Streptomyces kanasenisi ZX01 that was isolated from soil near Kanas Lake with significant bioactivity against tobacco mosaic virus. However, extremely low fermentation production has largely hindered further research and market applications on glycoprotein GP-1. In this study, response surface methodology was used to optimize fermentation conditions in a shake flask for higher glycoprotein GP-1 production. When the optimized fermentation conditions were inoculum volume of 6%, initial pH of 6...
January 10, 2018: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/29311639/exploring-viral-diversity-in-a-unique-south-african-soil-habitat
#9
Jane Segobola, Evelien Adriaenssens, Tsepo Tsekoa, Konanani Rashamuse, Don Cowan
The Kogelberg Biosphere Reserve in the Cape Floral Kingdom in South Africa is known for its unique plant biodiversity. The potential presence of unique microbial and viral biodiversity associated with this unique plant biodiversity led us to explore the fynbos soil using metaviromic techniques. In this study, metaviromes of a soil community from the Kogelberg Biosphere Reserve has been characterised in detail for the first time. Metaviromic DNA was recovered from soil and sequenced by Next Generation Sequencing...
January 8, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29177047/virucidal-efficacy-of-peracetic-acid-for-instrument-disinfection
#10
Britta Becker, Florian H H Brill, Daniel Todt, Eike Steinmann, Johannes Lenz, Dajana Paulmann, Birte Bischoff, Jochen Steinmann
Background: Various peracetic-acid (PAA)-based products for processing flexible endoscopes on the market are often based on a two-component system including a cleaning step before the addition of PAA as disinfectant. The peracetic acid concentrations in these formulations from different manufacturers are ranging from 400 to 1500 ppm (part per million). These products are used at temperatures between 20 °C and 37 °C. Since information on the virus-inactivating properties of peracetic acid at different concentrations and temperature is missing, it was the aim of the study to evaluate peracetic acid solutions against test viruses using the quantitative suspension test, EN 14476...
2017: Antimicrobial Resistance and Infection Control
https://www.readbyqxmd.com/read/29169833/molecular-characterization-of-variants-of-a-new-rule-breaking-tobacco-rattle-virus-rna2-in-potatoes
#11
Kerstin Lindner, Inga Hilbrich, Renate Koenig
In potato tubers showing pronounced corky ringspot symptoms, two related 'rule-breaking" tobacco rattle virus (TRV) RNA2s, named Da-2 and Db-2, were identified. Their coat protein (cp) genes are preceded on the 5"side by an additional gene for a 35kDa protein for which no relationships with previously described TRV genes or their expression products were found. With estimated 4296 and 4247 nucleotides (nts) the two RNAs are the longest TRV RNA2s described so far. The difference in size between Da-2 and Db-2 is due to a duplication of a stretch of 49 nts in the 5'untranslated region of Da-2...
November 20, 2017: Virus Research
https://www.readbyqxmd.com/read/29154906/increase-in-taxonomic-assignment-efficiency-of-viral-reads-in-metagenomic-studies
#12
S François, D Filloux, M Frayssinet, P Roumagnac, D P Martin, M Ogliastro, R Froissart
Metagenomics studies have revolutionized the field of biology by revealing the presence of many previously unisolated and uncultured micro-organisms. However, one of the main problems encountered in metagenomic studies is the high percentage of sequences that cannot be assigned taxonomically using commonly used similarity-based approaches (e.g. BLAST or HMM). These unassigned sequences are allegorically called « dark matter » in the metagenomic literature and are often referred to as being derived from new or unknown organisms...
November 14, 2017: Virus Research
https://www.readbyqxmd.com/read/29147662/effectiveness-of-a-lytic-phage-srg1-against-vancomycin-resistant-enterococcus-faecalis-in-compost-and-soil
#13
Sidra Rahmat Ullah, Saadia Andleeb, Taskeen Raza, Muhsin Jamal, Khalid Mehmood
Nosocomial infections caused by vancomycin-resistant Enterococcus have become a major problem. Bacteriophage therapy is proposed as a potential alternative therapy. Bacteriophages are viruses that infect bacteria and are ubiquitous in nature. Lytic bacteriophage was isolated from sewage water that infects VREF, the causative agent of endocarditis, bacteraemia, and urinary tract infections (UTIs). The phage produced clear plaques with unique clear morphology and well-defined boundaries. TEM results of phage revealed it to be 108 ± 0...
2017: BioMed Research International
https://www.readbyqxmd.com/read/29143925/production-of-fumonisins-by-endophytic-strains-of-tolypocladium-cylindrosporum-and-its-relation-to-fungal-virus-infection
#14
Iñigo Zabalgogeazcoa, Amador Alvarez, Noemi Herrero, Beatriz R Vazquez-de-Aldana
Fumonisins were first discovered in Fusarium verticillioides, a fungus associated to disease and asymptomatic infections in maize. Afterwards, other fungal taxa have been found to produce fumonisins. The entomopathogenic ascomycete Tolypocladium cylindrosporum has been isolated from soil and also as an endophyte from leaves of grasses. The objectives of this work were to determine the in vitro production of fumonisin B (FB) mycotoxins and the immunosuppressive compound cyclosporine A (CyA) in several strains of T...
November 16, 2017: Mycotoxin Research
https://www.readbyqxmd.com/read/29110758/apol1-renal-risk-variants-fertile-soil-for-hiv-associated-nephropathy
#15
REVIEW
Jeffrey B Kopp, Jurgen Heymann, Cheryl A Winkler
Apolipoprotein L1 (APOL1) genetic variants are potent risk factors for glomerular disease, but one or more additional factors are required for expression of glomerular disease. Uncontrolled or poorly controlled human immunodeficiency virus (HIV) infection is the most potent susceptibility factor for APOL1 nephropathy that has been identified to date. APOL1 variants are associated with HIV-associated nephropathy (HIVAN), a podocyte disease, but not with HIV-immune complex disease, primarily a disease of the mesangium...
November 2017: Seminars in Nephrology
https://www.readbyqxmd.com/read/29105037/an-update-of-the-worldwide-integrated-assessment-wia-on-systemic-insecticides-part-1-new-molecules-metabolism-fate-and-transport
#16
Chiara Giorio, Anton Safer, Francisco Sánchez-Bayo, Andrea Tapparo, Andrea Lentola, Vincenzo Girolami, Maarten Bijleveld van Lexmond, Jean-Marc Bonmatin
With the exponential number of published data on neonicotinoids and fipronil during the last decade, an updated review of literature has been conducted in three parts. The present part focuses on gaps of knowledge that have been addressed after publication of the Worldwide Integrated Assessment (WIA) on systemic insecticides in 2015. More specifically, new data on the mode of action and metabolism of neonicotinoids and fipronil, and their toxicity to invertebrates and vertebrates, were obtained. We included the newly detected synergistic effects and/or interactions of these systemic insecticides with other insecticides, fungicides, herbicides, adjuvants, honeybee viruses, and parasites of honeybees...
November 5, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/29067022/soil-viral-communities-vary-temporally-and-along-a-land-use-transect-as-revealed-by-virus-like-particle-counting-and-a-modified-community-fingerprinting-approach-frapd
#17
Anja Narr, Ali Nawaz, Lukas Y Wick, Hauke Harms, Antonis Chatzinotas
Environmental surveys on soil viruses are still rare and mostly anecdotal, i. e., they mostly report on viruses at one location or for only a few sampling dates. Detailed time-series analysis with multiple samples can reveal the spatio-temporal dynamics of viral communities and provide important input as to how viruses interact with their potential hosts and the environment. Such surveys, however, require fast, easy-to-apply and reliable methods. In the present study we surveyed monthly across 13 months the abundance of virus-like particles (VLP) and the structure of the viral communities in soils along a land use transect (i...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29034174/rare-case-of-rhizobium-radiobacter-bioprosthetic-mitral-valve-endocarditis
#18
Ryan Halas, Chris Jacob, Karun Badwal, Rafi Mir
Rhizobium radiobacter is an aerobic, gram negative, rod-shaped, bacterium typically found in the soil. Commonly a plant pathogen, it is also a rare human pathogen causing serious disease. Risk factors for infection include neutropenia, leukopenia, catheters, hospitalization, and low CD4+ lymphocyte count, especially in patients with malignancy or human immunodeficiency virus. There is currently limited literature to establish a definitive guideline for antimicrobial therapy and obtaining susceptibilities from a specialized laboratory is appropriate...
2017: IDCases
https://www.readbyqxmd.com/read/29029976/extraction-and-rt-qpcr-detection-of-enteroviruses-from-solid-environmental-matrixes-method-decision-tree-for-different-sample-types-and-viral-concentrations
#19
Julia Martín-Díaz, Francisco Lucena
Quantitative RT-PCR methods (RT-qPCR) are becoming increasingly desirable for the detection of enteric viruses in solid environmental matrixes such as sediments, soils and sewage sludge. However, effective methodologies that allow the extraction of high quality RNA ready for molecular quantification continue to be evaluated. In the present study, four different methods for enterovirus extraction from solid environmental matrixes were compared in terms of viral recovery and inhibitor removal. Three indirect methods based on glycine elution and concentration by ultracentrifugation were tested...
January 2018: Journal of Virological Methods
https://www.readbyqxmd.com/read/29027659/a-novel-cotton-wrky-gene-ghwrky6-like-improves-salt-tolerance-by-activating-the-aba-signalling-pathway-and-scavenging-of-reactive-oxygen-species
#20
Abid Ullah, Heng Sun, Hakim, Xiyan Yang, Xianlong Zhang
WRKY transcription factors are transcriptional regulators of signalling pathways involved in biotic and abiotic stress responses. In this study, we report that ectopic expression of the GhWRKY6-like gene significantly improved salt tolerance in Arabidopsis while silencing the GhWRKY6-like increase the sensitivity to abiotic stresses in cotton. GhWRKY6-like was localized to the nucleus. Expression of GhWRKY6-like was remarkably induced by salt, ethylene glycol (PEG) and abscisic acid (ABA) treatments. For further characterization, the GhWRKY6-like gene was cloned and transformed into Arabidopsis...
October 13, 2017: Physiologia Plantarum
keyword
keyword
73707
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"