keyword
https://read.qxmd.com/read/37762701/transcriptome-based-wgcna-analysis-reveals-the-mechanism-of-drought-resistance-differences-in-sweetpotato-ipomoea-batatas-l-lam
#21
JOURNAL ARTICLE
Jikai Zong, Peitao Chen, Qingqing Luo, Jilong Gao, Ruihua Qin, Chunli Wu, Qina Lv, Tengfei Zhao, Yufan Fu
Sweetpotato ( Ipomoea batatas (L.) Lam.) is a globally significant storage root crop, but it is highly susceptible to yield reduction under severe drought conditions. Therefore, understanding the mechanism of sweetpotato resistance to drought stress is helpful for the creation of outstanding germplasm and the selection of varieties with strong drought resistance. In this study, we conducted a comprehensive analysis of the phenotypic and physiological traits of 17 sweetpotato breeding lines and 10 varieties under drought stress through a 48 h treatment in a Hoagland culture medium containing 20% PEG6000...
September 21, 2023: International Journal of Molecular Sciences
https://read.qxmd.com/read/37745063/heterogeneity-characterization-of-hepatocellular-carcinoma-based-on-the-sensitivity-to-5-fluorouracil-and-development-of-a-prognostic-regression-model
#22
JOURNAL ARTICLE
Xinyu Gu, Shuang Li, Xiao Ma, Di Huang, Penghui Li
Background: 5-Fluorouracil (5-FU) is a widely used chemotherapeutic drug in clinical cancer treatment, including hepatocellular carcinoma (HCC). A correct understanding of the mechanisms leading to a low or lack of sensitivity of HCC to 5-FU-based treatment is a key element in the current personalized medical treatment. Methods: Weighted gene co-expression network analysis (WGCNA) was used to analyze the expression profiles of the cancer cell line from GDSC2 to identify 5-FU-related modules and hub genes. According to hub genes, HCC was classified and the machine learning model was developed by ConsensusClusterPlus and five different machine learning algorithms...
2023: Frontiers in Pharmacology
https://read.qxmd.com/read/37732164/ultrasound-assessment-of-gastric-content-in-patients-undergoing-laparoscopic-cholecystectomy-after-preoperative-oral-carbohydrates-a-prospective-randomized-controlled-double-blind-study
#23
JOURNAL ARTICLE
Yali Ge, Dejuan Shen, Yinyin Ding, Keting Wu, Yang Zhang
BACKGROUND: To evaluate the gastric volume and nature after drinking preoperative oral carbohydrates in patients undergoing laparoscopic cholecystectomy via ultrasonography. METHODS: One hundred patients who had been scheduled for elective laparoscopic cholecystectomy were enrolled and randomized into the traditional fasting group (Control group, n  = 50) and the carbohydrate group (CHO group, n  = 50). Patients in the Control group fasted solids and drink from midnight, the day before surgery...
2023: Frontiers in Surgery
https://read.qxmd.com/read/37587696/gene-duplications-facilitate-c4-cam-compatibility-in-common-purslane
#24
JOURNAL ARTICLE
Xiaoliang Wang, Xuxu Ma, Ge Yan, Lei Hua, Han Liu, Wei Huang, Zhikai Liang, Qing Chao, Julian M Hibberd, Yuannian Jiao, Mei Zhang
Common purslane (Portulaca oleracea) integrates both C4 and crassulacean acid metabolism (CAM) photosynthesis pathways, and is a promising model plant to explore C4-CAM plasticity. Here, we report a high-quality chromosome-level genome of NAD-ME subtype common purslane that provides evidence for two rounds of whole-genome duplication (WGD) with an ancient WGD (P-β) in the common ancestor to Portulacaceae and Cactaceae around 66.30 million years ago (Mya) and another (Po-α) specific to common purslane lineage around 7...
August 17, 2023: Plant Physiology
https://read.qxmd.com/read/37553699/randomized-controlled-trial-of-liberal-vs-standard-fasting-instructions-in-percutaneous-cardiac-procedures
#25
JOURNAL ARTICLE
Derek J Atkinson, Jamie L Romeiser, Ibrahim O Almasry, Henry J Tannous, Puja B Parikh, Elliott Bennett-Guerrero
BACKGROUND: Pre-procedural fasting to reduce aspiration risk is usual care prior to surgery requiring anesthesia. Prolonged fasting, however, can result in dehydration and may adversely affect patient experience and outcomes. Previous studies suggest that providing a supplemental beverage to patients undergoing cardiac and a variety of other surgical procedures improves patients' subjective assessment of thirst and hunger and potentially decreases the need for inotrope and vasopressor therapy...
August 8, 2023: Perioperative Medicine
https://read.qxmd.com/read/37536681/accelerometry-measured-movement-behaviors-in-middle-aged-and-older-adults-cross-sectional-analysis-of-the-elsa-brasil-study
#26
JOURNAL ARTICLE
Danilo de Paula, Inácio Crochemore-Silva, Rosane Harter Griep, Bruce Bartholow Duncan, Maria Inês Schmidt
BACKGROUND: Little investigation of accelerometry assessed movement behaviors and physical inactivity was carried out in middle-aged and older adults in low-middle-income countries. OBJECTIVE: Describe accelerometry-measured movement behaviors and prevalence of physical inactivity in middle-aged and older adults. METHODS: We collected raw accelerometry data during the third visit (2017-2019) of ELSA-Brasil, a large-scale multicenter Brazilian cohort...
August 3, 2023: Journal of Physical Activity & Health
https://read.qxmd.com/read/37533556/seaward-migration-and-larval-release-coincide-with-lunar-and-light-dark-cycles-in-supratidal-land-crabs-cardisoma-carnifex-and-epigrapsus-notatus
#27
JOURNAL ARTICLE
Chung-Chieh Chang, Kent A Hatch, Chia-Hsuan Hsu, Wenbe Hwang, Hung-Chang Liu, Yuan-Mou Chang
Herein we investigated the synchronous breeding migration and larval release of ovigerous females in two dominant supratidal land crabs Cardisoma carnifex and Epigrapsus notatus in the mixed semidiurnal tidal regime in Taijiang National Park (Tainan, Taiwan). We mainly focused on the monthly and daily rhythms during the breeding season of migration and larval release for these two crabs. We also sought to understand what the main environmental cues were for these monthly and diel rhythms. Both lunar and tidal amplitude cycles are potential proximate causes for the monthly lunar/semilunar reproductive rhythm in crabs...
2023: Zoological Studies (Taipei, Taiwan)
https://read.qxmd.com/read/37474098/does-exposure-to-moonlight-affect-day-night-changes-in-melatonin-and-metabolic-parameters-in-amazonian-fish
#28
JOURNAL ARTICLE
Ana Caroliny Cerdeira Lopes, Bruno Olivetti de Mattos, Jaydione Luiz Marcon, Luisa María Vera, José Fernando López-Olmeda, Francisco Javier Sánchez-Vázquez, Thaís Billalba Carvalho
Lunar cycle modulates the rhythmic activity patterns of many animals, including fish. The effect of the moonlight cycle on daily melatonin and metabolic parameters was evaluated in matrinxã (Brycon amazonicus) subjected to external natural lighting. Eighty juvenile were distributed in 4 tanks of 1m3 (20 fish/tank) and divided into two groups. One group was exposed to the full moon and the other group to the new moon for 30 days, which corresponds to the duration of the lunar period. At the end of the lunar phase, 6 fish from each group were anesthetized to collect blood, tissue and eye samples at midday and midnight...
July 18, 2023: Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology
https://read.qxmd.com/read/37448777/energetic-electron-precipitation-driven-by-electromagnetic-ion-cyclotron-waves-from-elfin-s-low-altitude-perspective
#29
REVIEW
V Angelopoulos, X-J Zhang, A V Artemyev, D Mourenas, E Tsai, C Wilkins, A Runov, J Liu, D L Turner, W Li, K Khurana, R E Wirz, V A Sergeev, X Meng, J Wu, M D Hartinger, T Raita, Y Shen, X An, X Shi, M F Bashir, X Shen, L Gan, M Qin, L Capannolo, Q Ma, C L Russell, E V Masongsong, R Caron, I He, L Iglesias, S Jha, J King, S Kumar, K Le, J Mao, A McDermott, K Nguyen, A Norris, A Palla, A Roosnovo, J Tam, E Xie, R C Yap, S Ye, C Young, L A Adair, C Shaffer, M Chung, P Cruce, M Lawson, D Leneman, M Allen, M Anderson, M Arreola-Zamora, J Artinger, J Asher, D Branchevsky, M Cliffe, K Colton, C Costello, D Depe, B W Domae, S Eldin, L Fitzgibbon, A Flemming, D M Frederick, A Gilbert, B Hesford, R Krieger, K Lian, E McKinney, J P Miller, C Pedersen, Z Qu, R Rozario, M Rubly, R Seaton, A Subramanian, S R Sundin, A Tan, D Thomlinson, W Turner, G Wing, C Wong, A Zarifian
We review comprehensive observations of electromagnetic ion cyclotron (EMIC) wave-driven energetic electron precipitation using data collected by the energetic electron detector on the Electron Losses and Fields InvestigatioN (ELFIN) mission, two polar-orbiting low-altitude spinning CubeSats, measuring 50-5000 keV electrons with good pitch-angle and energy resolution. EMIC wave-driven precipitation exhibits a distinct signature in energy-spectrograms of the precipitating-to-trapped flux ratio: peaks at >0...
2023: Space Science Reviews
https://read.qxmd.com/read/37425324/influencing-factors-associated-with-high-myopia-in-chinese-college-students
#30
JOURNAL ARTICLE
Weiran Zhang, Xiaoyun Hou, Chang Li, Sennan Wang, Nianen Liu, Yan Zhang, Zhiqing Li
BACKGROUND: High myopia (HM) may elicit irreversible pathological changes in the fundus and severely impair visual quality, thereby becoming a major public health issue in China. However, the influencing factors associated with HM remain unknown in Chinese college students, whose visual quality is crucial to country development. METHODS: This is a cross-sectional observational study. Two thousand three hundred and fifteen undergraduate and graduate students were initially recruited from various majors in 3 universities in Tianjin, China...
2023: Frontiers in Medicine
https://read.qxmd.com/read/37346206/effect-of-patient-age-on-timing-of-inpatient-esophagogastroduodenoscopy-and-outcomes-for-non-variceal-upper-gi-bleeds
#31
JOURNAL ARTICLE
Anmol Mittal, Faiz Afridi, Ayham Khrais, Sushil Ahlawat
Background Esophagogastroduodenoscopy (EGD) is typically performed within 24 hours of presentation for patients admitted to a hospital for patients presenting with a non-variceal upper gastrointestinal bleed (UGIB). To date, no studies have been performed to identify the impact of patient age on the timing of inpatient EGD and patient outcomes in non-variceal UGIB. Our aim was to assess the differences in the timing of EGD, blood transfusion requirements, development of hemorrhagic shock, development of acute renal failure, mortality, length of stay, and total hospital charges for patients aged 18-59 and those aged 60 and older...
May 2023: Curēus
https://read.qxmd.com/read/37330419/insulin-therapy-and-blood-glucose-management-in-critically-ill-patients-a-1-day-cross-sectional-observational-study-in-69-french-intensive-care-units
#32
JOURNAL ARTICLE
Maxime Desgrouas, Julien Demiselle, Laure Stiel, Vincent Brunot, Rémy Marnai, Sacha Sarfati, Maud Fiancette, Fabien Lambiotte, Arnaud W Thille, Maxime Leloup, Sébastien Clerc, Pascal Beuret, Anne-Astrid Bourion, Johan Daum, Rémi Malhomme, Ramin Ravan, Bertrand Sauneuf, Jean-Philippe Rigaud, Pierre-François Dequin, Thierry Boulain
BACKGROUND: Hyperglycaemia is common in critically ill patients, but blood glucose and insulin management may differ widely among intensive care units (ICUs). We aimed to describe insulin use practices and the resulting glycaemic control in French ICUs. We conducted a multicentre 1-day observational study on November 23, 2021, in 69 French ICUs. Adult patients hospitalized for an acute organ failure, severe infection or post-operative care were included. Data were recorded from midnight to 11:59 p...
June 17, 2023: Annals of Intensive Care
https://read.qxmd.com/read/37252134/evaluating-longitudinal-relationships-between-parental-monitoring-and-substance-use-in-a-multi-year-intensive-longitudinal-study-of-670-adolescent-twins
#33
JOURNAL ARTICLE
Jordan D Alexander, Samantha M Freis, Stephanie M Zellers, Robin Corley, Amy Ledbetter, Rachel K Schneider, Chanda Phelan, Hariharan Subramonyam, Maia Frieser, Gianna Rea-Sandin, Michelle E Stocker, Helen Vernier, Ming Jiang, Yan Luo, Qi Zhao, Sally Ann Rhea, John Hewitt, Monica Luciana, Matt McGue, Sylia Wilson, Paul Resnick, Naomi P Friedman, Scott I Vrieze
INTRODUCTION: Parental monitoring is a key intervention target for adolescent substance use, however this practice is largely supported by causally uninformative cross-sectional or sparse-longitudinal observational research designs. METHODS: We therefore evaluated relationships between adolescent substance use (assessed weekly) and parental monitoring (assessed every two months) in 670 adolescent twins for two years. This allowed us to assess how individual-level parental monitoring and substance use trajectories were related and, via the twin design, to quantify genetic and environmental contributions to these relationships...
2023: Frontiers in Psychiatry
https://read.qxmd.com/read/37239900/submergence-stress-alters-the-expression-of-clock-genes-and-configures-new-zeniths-and-expression-of-outputs-in-brachypodium-distachyon
#34
JOURNAL ARTICLE
Lucisabel Medina-Chávez, Christian Camacho, Jorge Arturo Martínez-Rodríguez, Blanca Estela Barrera-Figueroa, Dawn H Nagel, Piyada Juntawong, Julián Mario Peña-Castro
Plant networks of oscillating genes coordinate internal processes with external cues, contributing to increased fitness. We hypothesized that the response to submergence stress may dynamically change during different times of the day. In this work, we determined the transcriptome (RNA sequencing) of the model monocotyledonous plant, Brachypodium distachyon , during a day of submergence stress, low light, and normal growth. Two ecotypes of differential tolerance, Bd21 (sensitive) and Bd21-3 (tolerant), were included...
May 10, 2023: International Journal of Molecular Sciences
https://read.qxmd.com/read/37142417/the-relationship-between-%C3%AE-amylase-and-the-degradation-of-starch-temporarily-stored-in-rice-leaf-blades
#35
JOURNAL ARTICLE
Yu Sugimura, Hiroshi Fukayama, Hiroyasu Michiyama, Tatsuya Hirano
Starch is stored temporarily in the leaves during the day but degraded during the night. In this study, we investigated the relationship between diurnal changes in starch content in rice leaf blades and the mRNA levels of β-amylase genes. In addition to the known plastid-type β-amylases OsBAM2 and OsBAM3, OsBAM4 and OsBAM5 were also identified as plastid targeted proteins. In the leaf blades, starch contents, which reached its maximum at the end of day, showed two periods of marked decrease: from 18:00 to 21:00 and from 24:00 to 6:00...
May 4, 2023: Bioscience, Biotechnology, and Biochemistry
https://read.qxmd.com/read/37128537/a-survey-of-traumatic-brain-injuries-from-road-traffic-collisions-in-a-lower-middle-income-country
#36
JOURNAL ARTICLE
Muhammad Tariq Barki, Faiqa Filza, Almas F Khattak, Osama Bin Khalid, Mustafa Qazi, Humaira Gilani, Shahid Ayub, Muhammad Farooq
The burden of traumatic brain injury (TBI) from road traffic collisions (RTCs) is great in low-and middle-income countries (LMICs) due to shortfalls in preventative measures, and the lack of relevant, accurate data collection. To address this gap, we sought to study the epidemiology of TBI from RTCs in two LMIC neurosurgical centres in order to identify factors amenable to preventative strategies. A prospective survey of all adult and paediatric cases of TBI from RTCs admitted to Northwest General Hospital (NWGH) and Hayatabad Medical Complex (HMC) over a four-week period was carried out...
March 2023: Curēus
https://read.qxmd.com/read/37117393/minimal-requirements-for-iso15189-validation-and-accreditation-of-three-next-generation-sequencing-procedures-for-sars-cov-2-surveillance-in-clinical-setting
#37
JOURNAL ARTICLE
Céline Maschietto, Gaëtan Otto, Pauline Rouzé, Nicolas Debortoli, Benoît Bihin, Lesly Nyinkeu, Olivier Denis, Te-Din Huang, François Mullier, Pierre Bogaerts, Jonathan Degosserie
Rapid and recurrent breakthroughs of new SARS-CoV-2 strains (variants) have prompted public health authorities worldwide to set up surveillance networks to monitor the circulation of variants of concern. The use of next-generation sequencing technologies has raised the need for quality control assessment as required in clinical laboratories. The present study is the first to propose a validation guide for SARS-CoV-2 typing using three different NGS methods fulfilling ISO15189 standards. These include the assessment of the risk, specificity, accuracy, reproducibility, and repeatability of the methods...
April 28, 2023: Scientific Reports
https://read.qxmd.com/read/37081087/comparison-of-sars-cov-2-whole-genome-sequencing-using-tiled-amplicon-enrichment-and-bait-hybridization
#38
JOURNAL ARTICLE
Anita Koskela von Sydow, Carl Mårten Lindqvist, Naveed Asghar, Magnus Johansson, Martin Sundqvist, Paula Mölling, Bianca Stenmark
The severe acute respiratory syndrome coronavirus 2 (SARS‑CoV‑2) pandemic has led to extensive virological monitoring by whole genome sequencing (WGS). Investigating the advantages and limitations of different protocols is key when conducting population-level WGS. SARS-CoV-2 positive samples with Ct values of 14-30 were run using three different protocols: the Twist Bioscience SARS‑CoV‑2 protocol with bait hybridization enrichment sequenced with Illumina, and two tiled amplicon enrichment protocols, ARTIC V3 and Midnight, sequenced with Illumina and Oxford Nanopore Technologies, respectively...
April 20, 2023: Scientific Reports
https://read.qxmd.com/read/37064843/what-would-be-the-difference-between-operative-treatment-of-patients-with-tibia-fractures-out-of-working-hours-intramedullary-nailing-for-tibial-shaft-fractures
#39
JOURNAL ARTICLE
Mehmet Selcuk Saygili, Ali Cagri Tekin, Tugrul Ergun, Baris Ozkul, Deniz Akbulut, Mustafa Alper Incesoy, Bilal Demir
OBJECTIVES: Performing orthopedic surgery in and out of working hours may affect the success of the surgery. Timing of surgery in tibial shaft fractures is controversial. In this study, the effect of the timing of surgery on the success and complications of intramedullary nailing of tibial fractures is examined. METHODS: Archieves of patients with tibial shaft fractures treated with reamed intramedullary nails between 2010 and 2016 were retrospectively analyzed...
2023: Şişli Etfal Hastanesi tıp bülteni
https://read.qxmd.com/read/37025759/the-impacts-of-air-quality-and-secondary-organic-aerosols-formation-on-traffic-accidents-in-heavy-fog-haze-weather
#40
JOURNAL ARTICLE
Chen-Wei Liang, Chia-Chun Chang, Jeng-Jong Liang
This study estimated changes in the levels of three components of regional haze, namely fine particulate matter (PM2.5 ), relative humidity (RH), and secondary organic aerosols (SOAs), at the time of two severe traffic accidents on a coastal expressway and a freeway in the Jianan Plain in southwestern Taiwan to understand the impact of weather and air quality factors on the low visibility. Monitoring data and surveillance images from four nearby air quality monitoring stations were collected to determine the precise causes of the poor visibility-related accidents...
April 2023: Heliyon
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