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Hydrolysed collagen

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https://www.readbyqxmd.com/read/29329864/antioxidant-and-anti-freezing-peptides-from-salmon-collagen-hydrolysate-prepared-by-bacterial-extracellular-protease
#1
RiBang Wu, CuiLing Wu, Dan Liu, XingHao Yang, JiaFeng Huang, Jiang Zhang, Binqiang Liao, HaiLun He
Extracted salmon skin collagen was hydrolysed with the free or immobilized extracellular protease of Vibrio sp. SQS2-3. The hydrolysate exhibited anti-freezing activity (>3 kDa) and antioxidant activity (<3000 Da) after ultrafiltration. The antioxidant peptide was further purified by size-exclusion chromatography and found to scavenge DPPH (73.29 ± 1.03%), OH (72.73 ± 3.34%,), and intracellular ROS in HUVECs; protect DNA against oxidation-induced damage; and have an ORAC of 2.78 ± 0...
May 15, 2018: Food Chemistry
https://www.readbyqxmd.com/read/29310774/measurement-of-elastin-collagen-and-total-protein-levels-in-tissues
#2
Ivan Stoilov, Barry C Starcher, Robert P Mecham, Thomas J Broekelmann
Elastin and collagen levels in tissues are frequently difficult to measure because of each protein's limited solubility. This chapter provides detailed methodology for the determination of elastin, collagen, and total protein levels in a single tissue sample. All three assays start with an acid hydrolysate of the tissue, which breaks the tissue-associated proteins down to their component amino acids. Marker amino acids unique to each protein (desmosine for elastin and hydroxyproline for collagen) are then quantified...
2018: Methods in Cell Biology
https://www.readbyqxmd.com/read/29287404/freeze-dried-phosphatidylcholine-liposomes-encapsulating-various-antioxidant-extracts-from-natural-waste-as-functional-ingredients-in-surimi-gels
#3
D Marín, A Alemán, A Sánchez-Faure, P Montero, M C Gómez-Guillén
Three antioxidant extracts (collagen hydrolysate, pomegranate peel extract, shrimp lipid extract) were encapsulated in soy phosphatidylcholine liposomes with the addition of glycerol. The particle size of the fresh liposomes ranged from 75.7 to 81.0 nm and zeta potential from -64.6 to -88.2 mV. Freeze-drying increased particle size (199-283 nm), and slightly decreased zeta potential. The lyophilized liposomes were incorporated in squid surimi gels at 10.5% concentration. An alternative functional formulation was also prepared by adding 2% of non-encapsulated bioactive extract...
April 15, 2018: Food Chemistry
https://www.readbyqxmd.com/read/29274418/extraction-purification-and-characterization-of-low-molecular-weight-proline-iminopeptidase-from-probiotic-l-plantarum-for-meat-tenderization
#4
Preeti Chanalia, Dimpi Gandhi, Pooja Attri, Suman Dhanda
Membrane bound proline iminopeptidase (PIP) from lactic acid bacteria (LAB) L. plantarum was extracted and purified using CM-sephadex, Sephadex G-100 and Q-sepharose column chromatography. PIP was purified with purification fold 7.13 and 33.5% yield. SDS-PAGE and MALDI-TOF revealed it as homodimer with molecular weight of 37.9 kDa and subunit of mass 18.9 kDa. Purified enzyme exhibited maximum activity at 45 °C and pH 7.0. Km and Vmax of purified PIP were 65 μM and 25.9 nm/min/ml respectively. Inhibition by PMSF confirmed it a serine protease...
December 20, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/29272917/purification-and-characterization-of-peptides-inhibiting-mmp-1-activity-with-c-terminate-of-gly-leu-from-simulated-gastrointestinal-digestion-hydrolysates-of-tilapia-oreochromis-niloticus-skin-gelatin
#5
Sun Liping, Liu Qiuming, Fan Jian, Li Xiao, Zhuang Yongliang
Tilapia skin gelatin hydrolysates (TSGH) were prepared by simulated gastrointestinal digestion and separated by gel filtration and semipreparative reverse phase-high performance liquid chromatography. The antiphotoaging effects were evaluated using an ultraviolet radiation B (UVB)-induced mouse embryonic fibroblasts (MEFs) photoaging model in vitro. Three fractions from TSGH with high inhibitory intercellular matrix metalloproteinases-1 (MMP-1) activities and reactive oxygen species (ROS) production were obtained...
December 22, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/29207560/optimization-of-collagenase-production-by-pseudoalteromonas-sp-sjn2-and-application-of-collagenases-in-the-preparation-of-antioxidative-hydrolysates
#6
Xinghao Yang, Xiao Xiao, Dan Liu, Ribang Wu, Cuiling Wu, Jiang Zhang, Jiafeng Huang, Binqiang Liao, Hailun He
Collagenases are the most important group of commercially-produced enzymes. However, even though biological resources are abundant in the sea, very few of these commercially popular enzymes are from marine sources, especially from marine bacteria. We optimized the production of marine collagenases by Pseudoalteromonas sp. SJN2 and investigated the antioxidant activities of the hydrolysates. Media components and culture conditions associated with marine collagenase production by Pseudoalteromonas sp. SJN2 were optimized by statistical methods, namely Plackett-Burman design and response surface methodology (RSM)...
December 4, 2017: Marine Drugs
https://www.readbyqxmd.com/read/29197053/film-forming-ability-of-collagen-hydrolysate-extracted-from-leather-solid-wastes-with-chitosan
#7
Buğra Ocak
Discharges of huge quantities of leather solid wastes by leather industries and the increased use of synthetic packaging films have raised serious concerns on account of their environmental impacts. The paper focuses on the development and characterization of potential environmentally friendly composite films using collagen hydrolysate (CH) extracted from leather solid wastes and chitosan (C) to assess the feasibility of producing polymeric materials suitable for applications in packaging and wrapping purposes...
December 1, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/29146927/preparation-and-functional-evaluation-of-collagen-oligopeptide-rich-hydrolysate-from-fish-skin-with-the-serine-collagenolytic-protease-from-pseudoalteromonas-sp-sm9913
#8
Xiu-Lan Chen, Ming Peng, Jing Li, Bai-Lu Tang, Xuan Shao, Fang Zhao, Chang Liu, Xi-Ying Zhang, Ping-Yi Li, Mei Shi, Yu-Zhong Zhang, Xiao-Yan Song
Although several serine collagenolytic proteases from bacteria were reported, none has been used to prepare bioactive collagen peptides. MCP-01 is the most abundant extracellular protease of deep-sea Pseudoalteromonas sp. SM9913 and is a serine collagenolytic protease with high efficiency on fish collagen hydrolysis. Here, we set up a pilot scale process to ferment SM9913 for extracellular protease production. With SM9913 extracellular protease as a tool, a process to prepare collagen oligopeptide-rich hydrolysate from codfish skin was set up, which was further scaled up to pilot (100 L) and plant (2000 L) levels with yields >66%...
November 16, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29114654/the-presence-of-food-derived-collagen-peptides-in-human-body-structure-and-biological-activity
#9
REVIEW
Kenji Sato
It has been demonstrated that the ingestion of some protein hydrolysates exerts health-promoting effects. For understanding the underlying mechanisms responsible for these effects, the identification of bioactive peptides in the target organ is crucial. For this purpose, in vitro activity-guided fractionation for peptides in the protein hydrolysate has been performed. However, the peptides in the hydrolysate may be further degraded during digestion. The concentration of the active peptides, which were identified by in vitro activity-guided fractionation, in human blood is frequently very low (nanomolar levels)...
November 8, 2017: Food & Function
https://www.readbyqxmd.com/read/29090707/isolation-and-identification-of-calcium-chelating-peptides-from-pacific-cod-skin-gelatin-and-their-binding-properties-with-calcium
#10
Wenfei Wu, Bafang Li, Hu Hou, Hongwei Zhang, Xue Zhao
A calcium-chelating peptide is considered to have the ability to improve calcium absorption. In this study, Pacific cod skin gelatin hydrolysates treated with trypsin for 120 min exhibited higher calcium-chelating activity. Sequential chromatography, involving hydroxyapatite affinity chromatography and reversed phase high performance liquid chromatography, was used for the purification of calcium-chelating peptides. Two novel peptides with the typical characteristics of collagen were sequenced as GDKGESGEAGER and GEKGEGGHR based on LC-HRMS/MS, which showed a high affinity to calcium...
November 1, 2017: Food & Function
https://www.readbyqxmd.com/read/29037731/sequential-extraction-of-gel-forming-proteins-collagen-and-collagen-hydrolysate-from-gutted-silver-carp-hypophthalmichthys-molitrix-a-biorefinery-approach
#11
Mehdi Abdollahi, Masoud Rezaei, Ali Jafarpour, Ingrid Undeland
Collagen and collagen hydrolysate (CH) was recovered from the bone and skin containing sediment residue emerging during pH-shift-based protein isolation from silver carp. Hydrolysis resulted in higher yield (15.1-15.4%) compared to collagen isolation by acid or pepsin (3.1-5.9%) (p<0.05). Isolated collagens were characterized as type I and maintained their triple-helical structure, confirmed by SDS-PAGE and FTIR. Pepsin-hydrolysis and sequential hydrolysis by pepsin and trypsin hydrolyzed all heavy molecular weight chains of collagen but sequential hydrolysis yielded higher degree of hydrolysis...
March 1, 2018: Food Chemistry
https://www.readbyqxmd.com/read/29037671/blue-mussel-mytilus-edulis-protein-hydrolysate-promotes-mouse-mesenchymal-stem-cell-differentiation-into-osteoblasts-through-up-regulation-of-bone-morphogenetic-protein
#12
Jun-Ho Hyung, Chang-Bum Ahn, Jae-Young Je
Seafood provides a range of health benefits due to its high-protein level. In this study, the osteogenic effect of blue mussel (Mytilus edulis) protein hydrolysates (BMPH) on osteoblast differentiation were examined using mouse mesenchymal stem cells (MSCs). A preparation we called BMPH<1kDa which showed the highest osteogenic effect in MSCs, was prepared by peptic hydrolysis. BMPH<1kDa treatment stimulated osteoblast differentiation with alkaline phosphatase (ALP) induction, osteocalcin and type I collagen activity as well as calcium deposition...
March 1, 2018: Food Chemistry
https://www.readbyqxmd.com/read/29034640/-preparation-and-antioxidant-activity-detection-of-collagen-peptide-from-cirrhinus-molitorella-skin
#13
Cuiling Wu, Ribang Wu, Dan Liu, Xinghao Yang, Jiang Zhang, Jiafeng Huang, Hailun He
In order to prepare antioxidant peptide through hydrolyzing low-value protein resources with bacterial extracellular proteases and to discover novel proteases, crude extracellular protease from Pseudoalteromonas sp. SHK1-2 was obtained through fermentation which was used to hydrolyze collagen extracted from Cirrhinus molitorella skin. Small peptide fraction was isolated from hydrolysate by ultrafiltration and Sephadex LH-20 size exclusion chromatography and showed 1, 1-diphenyl-2-picrylhydrazyl radical scavenging activity (35...
December 25, 2016: Sheng Wu Gong Cheng Xue Bao, Chinese Journal of Biotechnology
https://www.readbyqxmd.com/read/29018060/dietary-supplements-for-treating-osteoarthritis-a-systematic-review-and-meta-analysis
#14
REVIEW
Xiaoqian Liu, Gustavo C Machado, Jillian P Eyles, Varshini Ravi, David J Hunter
OBJECTIVE: To investigate the efficacy and safety of dietary supplements for patients with osteoarthritis. DESIGN: An intervention systematic review with random effects meta-analysis and meta-regression. DATA SOURCES: MEDLINE, EMBASE, Cochrane Register of Controlled Trials, Allied and Complementary Medicine and Cumulative Index to Nursing and Allied Health Literature were searched from inception to April 2017. STUDY ELIGIBILITY CRITERIA: Randomised controlled trials comparing oral supplements with placebo for hand, hip or knee osteoarthritis...
October 10, 2017: British Journal of Sports Medicine
https://www.readbyqxmd.com/read/28990520/collagenolytic-enzymes-and-their-applications-in-biomedicine
#15
Anatoly Borisovich Shekhter, Anastasia V Balakireva, Natalia V Kuznetsova, Marina N Vukolova, Petr F Litvitsky, Andrey A Zamyatnin
Nowadays, enzymatic therapy is a very promising line of treatment for many different diseases. There is a group of disorders and conditions, caused by fibrotic and scar processes and associated with the excessive accumulation of collagen that needs to be catabolized to normalize the connective tissue content. The human body normally synthesizes special extracellular enzymes, matrix metalloproteases (MMPs) by itself. These enzymes can cleave components of extracellular matrix (ECM) and different types of collagen and thus maintain the balance of the connective tissue components...
October 6, 2017: Current Medicinal Chemistry
https://www.readbyqxmd.com/read/28988478/identification-of-collagen-derived-hydroxyproline-hyp-containing-cyclic-dipeptides-with-high-oral-bioavailability-efficient-formation-of-cyclo-x-hyp-from-x-hyp-gly-type-tripeptides-by-heating
#16
Yuki Taga, Masashi Kusubata, Kiyoko Ogawa-Goto, Shunji Hattori
Cyclic dipeptides (2,5-diketopiperazines) are present in a variety of foods and are reported to demonstrate antioxidant, antidepressant, and other beneficial effects. We recently developed a novel collagen hydrolysate characterized by a high content of X-hydroxyproline (Hyp)-Gly-type tripeptides using ginger protease. In the present study, we found that, through heating, X-Hyp-Gly can be easily converted into Hyp-containing cyclic dipeptides. After heating for 3 h at 85 °C and pH 4.8, Ala-Hyp-Gly was almost completely cyclized to cyclo(Ala-Hyp), in contrast to a slight cyclization of Ala-Hyp...
October 19, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28914450/changes-in-composition-and-content-of-food-derived-peptide-in-human-blood-after-daily-ingestion-of-collagen-hydrolysate-for-4-weeks
#17
Yasutaka Shigemura, Asahi Suzuki, Mihoko Kurokawa, Yosio Sato, Kenji Sato
BACKGROUND: daily ingestion of collagen hydrolysate improves skin and joint conditions. It has been speculated that the beneficial effects are exerted by food-derived hydroxyproline (Hyp) peptides, which are detected in human blood after single ingestions. In the present study, to investigate the detailed relationship of the beneficial effects of Hyp-peptides, we examined the concentrations of Hyp-peptides in human blood before and after daily ingestion. RESULTS: Hyp-peptides increased to a maximum level at 1 h after ingestion and reverted to their initial levels within 24 h during daily ingestion...
September 15, 2017: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/28906033/collagen-peptides-modulates-the-metabolism-of-extracellular-matrix-by-human-dermal-fibroblasts-derived-from-sun-protected-and-sun-exposed-body-sites
#18
Vivian Zague, Jonatas Bussador do Amaral, Paula Rezende Teixeira, Evandro Luis de Oliveira Niero, Camila Lauand, Glaucia Maria Machado-Santelli
Clinical data published in recent years has demonstrated positive effects of collagen hydrolysate (CH) on skin aging clinical signs. CH use as food supplement has a long history; however, few studies have addressed the underlying purpose of CH on the cellular and molecular biology of skin cells that could elucidate clinical improvement findings. Wide diversity of characteristics has been reported for dermal fibroblasts derived from different body sites and it is unknown whether collagen peptides could modulate differently cells from chronological aged and photoaged skin areas...
September 14, 2017: Cell Biology International
https://www.readbyqxmd.com/read/28831668/fish-collagen-hydrolysates-modulate-cartilage-metabolism
#19
Kanchanit Boonmaleerat, Orawan Wanachewin, Thanyaluck Phitak, Peraphan Pothacharoen, Prachya Kongtawelert
Osteoarthritis is a degenerative joint disease in which interleukin-1β plays a major role in the inflammatory process. Administration of collagen hydrolysate was an optional treatment of osteoarthritis. Fish has become an interesting source of collagen hydrolysate because of religious reason and there is no risk from mad cow disease. However, the effects of different sizes of fish collagen hydrolysate on cartilage and chondrocyte metabolism have not been well studied yet. This study examined the effect of different sizes of fish collagen hydrolysate on cartilage metabolism...
August 22, 2017: Cell Biochemistry and Biophysics
https://www.readbyqxmd.com/read/28808182/determination-of-serum-biomarkers-in-osteoarthritis-patients-a-previous-interventional-imaging-study-revisited
#20
Timothy McAlindon, Eckart Bartnik, Janina S Ried, Lenore Teichert, Matthias Herrmann, Klaus Flechsenhar
To evaluate in an interventional trial on knee osteoarthritis (OA) the level and change of two serum biomarkers and their correlation with imaging parameters. The previously reported interventional OA study (ClinicalTrials.gov: NCT00536302) identified a positive effect of collagen hydrolysate (CH) on cartilage morphology in patients with knee OA using delayed gadolinium enhanced magnetic resonance imaging (dGEMRIC). It was the objective in this research project to evaluate in an interventional clinical trial on knee OA the level and change of two serum biomarkers and their correlation with imaging parameters...
October 17, 2016: Journal of Biomedical Research
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