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Hydrolysed collagen

Samuel G Impey, Kelly M Hammond, Robert Naughton, Carl Langan-Evans, Sam O Shepherd, Adam P Sharples, Jessica Cegielski, Kenneth Smith, Stewart Jeromson, D Lee Hamilton, Graeme L Close, James P Morton
We examined the effects of whey versus collagen protein on skeletal muscle cell signalling responses associated with mitochondrial biogenesis and protein synthesis in recovery from an acute training session completed with low carbohydrate (CHO) availability. In a repeated measures design (after adhering to a 36-h exercise-dietary intervention to standardise pre-exercise muscle glycogen), eight males completed a 75-min non-exhaustive cycling protocol and consumed 22 g of a hydrolysed collagen blend (COLLAGEN) or whey (WHEY) protein 45 min prior to exercise, 22 g during exercise and 22 g immediately post-exercise...
May 14, 2018: International Journal of Sport Nutrition and Exercise Metabolism
Ana Sanchez, Maria Blanco, Begoña Correa, Ricardo I Perez-Martin, Carmen G Sotelo
Fish discards and subproducts may represent an important source of raw material, not only for the food industry, but for other different kind of industries, such as the nutraceutical and cosmetic industries. Collagen, which is mainly obtained from animal skins, is an important structural protein in the animal kingdom having many different applications. It is well known that fish skins constitute a significant subproduct in the fishery industry, especially in the case of some species, where fish skins may represent up to 20% of the total body weight of fish...
April 26, 2018: Marine Drugs
Agata Markiewicz, Malwina Zasada, Anna Erkiert-Polguj, Marzena Wieckowska-Szakiel, Elzbieta Budzisz
BACKGROUND: Mature skin is characterized by a loss of elasticity, hyperpigmentation, and dehydration. L-ascorbic acid stimulates the synthesis of collagen type I, inhibits melanogenesis, and helps to maintain correct skin hydration. Combining microneedle mesotherapy with the application of preparations rich in vitamin C results in better therapeutic effects due to the improved absorption of active substances. The study evaluates the effectiveness of the application of strawberry hydrolysate enriched with L-ascorbic acid using microneedle mesotherapy...
April 16, 2018: Journal of Cosmetic Dermatology
Zi-Qiang Liu, Feng-Yan Tuo, Liang Song, Yu-Xin Liu, Xiu-Ping Dong, Dong-Mei Li, Da-Yong Zhou, Fereidoon Shahidi
Trypsin, a representative serine proteinase, was used to hydrolyse the collagen fibres from sea cucumber (Stichopus japonicus) to highlight the role of serine proteinase in the autolysis of sea cucumber. Partial disaggregation of collagen fibres into collagen fibrils upon trypsin treatment occurred. The trypsin treatment also caused a time-dependent release of water-soluble glycosaminoglycans and proteins. Therefore, the degradation of the proteoglycan bridges between collagen fibrils might account for the disaggregation of collagen fibrils...
August 1, 2018: Food Chemistry
Qingyu Ma, Qiuming Liu, Ling Yuan, Yongliang Zhuang
A previous study has shown that tilapia fish skin gelatin hydrolysates inhibited photoaging in vivo, and that, Leu-Ser-Gly-Tyr-Gly-Pro (LSGYGP) identified in the hydrolysate had a high hydroxyl radical scavenging activity. In this study, activities of LSGYGP were further evaluated using ultraviolet B (UVB)-induced mouse embryonic fibroblasts (MEFs). UVB irradiation significantly increased the intercellular reactive oxygen species (ROS) production and matrix metalloproteinases (MMPs) activities and decreased the content of collagen in MEFs...
March 28, 2018: Nutrients
Yuanyuan Zhao, Zhanyong Wang, Jing Zhang, Tingting Su
The production of Hasma generates plentiful non-edible by-products in China and Central Asia. As one of main by-products, the skin of Rana chensinensis is discarded as waste without utilization. In this work, R. chensinensis skin collagen (RCSC) hydrolysates were extracted using pepsin under acidic conditions. The yield of RCSC is 15.1% (w/w). Amino acid analysis revealed that RCSC contained glycine (204.5/1000 residues) and imino acids (182/1000 residues). RCSC exhibited high solubility in acidic pH (1-4) and low NaCl concentrations (< 2%, w/v)...
March 2018: 3 Biotech
Lucie Jedličková, Hana Dvořáková, Jan Dvořák, Martin Kašný, Lenka Ulrychová, Jiří Vorel, Vojtěch Žárský, Libor Mikeš
BACKGROUND: Cysteine peptidases of clan CA, family C1 account for a major part of proteolytic activity in the haematophagous monogenean Eudiplozoon nipponicum. The full spectrum of cysteine cathepsins is, however, unknown and their particular biochemical properties, tissue localisation, and involvement in parasite-host relationships are yet to be explored. METHODS: Sequences of cathepsins L and B (EnCL and EnCB) were mined from E. nipponicum transcriptome and analysed bioinformatically...
March 6, 2018: Parasites & Vectors
Yoshihiro Osawa, Takafumi Mizushige, Sayuri Jinno, Fumihito Sugihara, Naoki Inoue, Hideyuki Tanaka, Yukihito Kabuyama
A number of studies have shown that oral administration of collagen hydrolysate (CH) results in the absorption of di- and tri-peptides. In order to understand the dynamics of CH absorption and metabolism, molecular profiles of hydroxyproline (Hyp) and Hyp-containing peptides (HCPs) were analyzed by in situ perfusion of rat intestine and liver. The total amount of absorbed HCPs during 1 h of perfusion was 16.6 μmol, which was significantly higher than that of free Hyp (6.6 μmol). In addition, HCPs were also reliably detected in hepatic perfusate at the level higher than free Hyp...
2018: Biomedical Research
Seungkuk Ahn, Christophe O Chantre, Alanna R Gannon, Johan U Lind, Patrick H Campbell, Thomas Grevesse, Blakely B O'Connor, Kevin Kit Parker
Historically, soy protein and extracts have been used extensively in foods due to their high protein and mineral content. More recently, soy protein has received attention for a variety of its potential health benefits, including enhanced skin regeneration. It has been reported that soy protein possesses bioactive molecules similar to extracellular matrix (ECM) proteins and estrogen. In wound healing, oral and topical soy has been heralded as a safe and cost-effective alternative to animal protein and endogenous estrogen...
January 23, 2018: Advanced Healthcare Materials
RiBang Wu, CuiLing Wu, Dan Liu, XingHao Yang, JiaFeng Huang, Jiang Zhang, Binqiang Liao, HaiLun He
Extracted salmon skin collagen was hydrolysed with the free or immobilized extracellular protease of Vibrio sp. SQS2-3. The hydrolysate exhibited anti-freezing activity (>3 kDa) and antioxidant activity (<3000 Da) after ultrafiltration. The antioxidant peptide was further purified by size-exclusion chromatography and found to scavenge DPPH (73.29 ± 1.03%), OH (72.73 ± 3.34%,), and intracellular ROS in HUVECs; protect DNA against oxidation-induced damage; and have an ORAC of 2.78 ± 0...
May 15, 2018: Food Chemistry
Ivan Stoilov, Barry C Starcher, Robert P Mecham, Thomas J Broekelmann
Elastin and collagen levels in tissues are frequently difficult to measure because of each protein's limited solubility. This chapter provides detailed methodology for the determination of elastin, collagen, and total protein levels in a single tissue sample. All three assays start with an acid hydrolysate of the tissue, which breaks the tissue-associated proteins down to their component amino acids. Marker amino acids unique to each protein (desmosine for elastin and hydroxyproline for collagen) are then quantified...
2018: Methods in Cell Biology
D Marín, A Alemán, A Sánchez-Faure, P Montero, M C Gómez-Guillén
Three antioxidant extracts (collagen hydrolysate, pomegranate peel extract, shrimp lipid extract) were encapsulated in soy phosphatidylcholine liposomes with the addition of glycerol. The particle size of the fresh liposomes ranged from 75.7 to 81.0 nm and zeta potential from -64.6 to -88.2 mV. Freeze-drying increased particle size (199-283 nm), and slightly decreased zeta potential. The lyophilized liposomes were incorporated in squid surimi gels at 10.5% concentration. An alternative functional formulation was also prepared by adding 2% of non-encapsulated bioactive extract...
April 15, 2018: Food Chemistry
Preeti Chanalia, Dimpi Gandhi, Pooja Attri, Suman Dhanda
Membrane bound proline iminopeptidase (PIP) from lactic acid bacteria (LAB) L. plantarum was extracted and purified using CM-sephadex, Sephadex G-100 and Q-sepharose column chromatography. PIP was purified with purification fold 7.13 and 33.5% yield. SDS-PAGE and MALDI-TOF revealed it as homodimer with molecular weight of 37.9 kDa and subunit of mass 18.9 kDa. Purified enzyme exhibited maximum activity at 45 °C and pH 7.0. Km and Vmax of purified PIP were 65 μM and 25.9 nm/min/ml respectively. Inhibition by PMSF confirmed it a serine protease...
April 1, 2018: International Journal of Biological Macromolecules
Sun Liping, Liu Qiuming, Fan Jian, Li Xiao, Zhuang Yongliang
Tilapia skin gelatin hydrolysates (TSGHs) were prepared by simulated gastrointestinal digestion and separated by gel filtration and semi-preparative reversed-phase high-performance liquid chromatography. The anti-photoaging effects were evaluated using an ultraviolet radiation B (UVB)-induced mouse embryonic fibroblast (MEF) photoaging model in vitro. Three fractions from TSGHs with high inhibitory intercellular matrix metalloproteinase-1 (MMP-1) activities and reactive oxygen species (ROS) production were obtained...
January 24, 2018: Journal of Agricultural and Food Chemistry
Xinghao Yang, Xiao Xiao, Dan Liu, Ribang Wu, Cuiling Wu, Jiang Zhang, Jiafeng Huang, Binqiang Liao, Hailun He
Collagenases are the most important group of commercially-produced enzymes. However, even though biological resources are abundant in the sea, very few of these commercially popular enzymes are from marine sources, especially from marine bacteria. We optimized the production of marine collagenases by Pseudoalteromonas sp. SJN2 and investigated the antioxidant activities of the hydrolysates. Media components and culture conditions associated with marine collagenase production by Pseudoalteromonas sp. SJN2 were optimized by statistical methods, namely Plackett-Burman design and response surface methodology (RSM)...
December 4, 2017: Marine Drugs
Buğra Ocak
Discharges of huge quantities of leather solid wastes by leather industries and the increased use of synthetic packaging films have raised serious concerns on account of their environmental impacts. The paper focuses on the development and characterization of potential environmentally friendly composite films using collagen hydrolysate (CH) extracted from leather solid wastes and chitosan (C) to assess the feasibility of producing polymeric materials suitable for applications in packaging and wrapping purposes...
February 2018: Environmental Science and Pollution Research International
Xiu-Lan Chen, Ming Peng, Jing Li, Bai-Lu Tang, Xuan Shao, Fang Zhao, Chang Liu, Xi-Ying Zhang, Ping-Yi Li, Mei Shi, Yu-Zhong Zhang, Xiao-Yan Song
Although several serine collagenolytic proteases from bacteria were reported, none has been used to prepare bioactive collagen peptides. MCP-01 is the most abundant extracellular protease of deep-sea Pseudoalteromonas sp. SM9913 and is a serine collagenolytic protease with high efficiency on fish collagen hydrolysis. Here, we set up a pilot scale process to ferment SM9913 for extracellular protease production. With SM9913 extracellular protease as a tool, a process to prepare collagen oligopeptide-rich hydrolysate from codfish skin was set up, which was further scaled up to pilot (100 L) and plant (2000 L) levels with yields >66%...
November 16, 2017: Scientific Reports
Kenji Sato
It has been demonstrated that the ingestion of some protein hydrolysates exerts health-promoting effects. For understanding the underlying mechanisms responsible for these effects, the identification of bioactive peptides in the target organ is crucial. For this purpose, in vitro activity-guided fractionation for peptides in the protein hydrolysate has been performed. However, the peptides in the hydrolysate may be further degraded during digestion. The concentration of the active peptides, which were identified by in vitro activity-guided fractionation, in human blood is frequently very low (nanomolar levels)...
December 13, 2017: Food & Function
Wenfei Wu, Bafang Li, Hu Hou, Hongwei Zhang, Xue Zhao
A calcium-chelating peptide is considered to have the ability to improve calcium absorption. In this study, Pacific cod skin gelatin hydrolysates treated with trypsin for 120 min exhibited higher calcium-chelating activity. Sequential chromatography, involving hydroxyapatite affinity chromatography and reversed phase high performance liquid chromatography, was used for the purification of calcium-chelating peptides. Two novel peptides with the typical characteristics of collagen were sequenced as GDKGESGEAGER and GEKGEGGHR based on LC-HRMS/MS, which showed a high affinity to calcium...
December 13, 2017: Food & Function
Mehdi Abdollahi, Masoud Rezaei, Ali Jafarpour, Ingrid Undeland
Collagen and collagen hydrolysate (CH) was recovered from the bone and skin containing sediment residue emerging during pH-shift-based protein isolation from silver carp. Hydrolysis resulted in higher yield (15.1-15.4%) compared to collagen isolation by acid or pepsin (3.1-5.9%) (p<0.05). Isolated collagens were characterized as type I and maintained their triple-helical structure, confirmed by SDS-PAGE and FTIR. Pepsin-hydrolysis and sequential hydrolysis by pepsin and trypsin hydrolyzed all heavy molecular weight chains of collagen but sequential hydrolysis yielded higher degree of hydrolysis...
March 1, 2018: Food Chemistry
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