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Hydrolysed collagen

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https://www.readbyqxmd.com/read/27932548/total-control-of-chromium-in-tanneries-thermal-decomposition-of-filtration-cake-from-enzymatic-hydrolysis-of-chrome-shavings
#1
P Kocurek, K Kolomazník, M Bařinová, J Hendrych
This paper deals with the problem of chromium recovery from chrome-tanned waste and thus with reducing the environmental impact of the leather industry. Chrome-tanned waste was transformed by alkaline enzymatic hydrolysis promoted by magnesium oxide into practically chromium-free, commercially applicable collagen hydrolysate and filtration cake containing a high portion of chromium. The crude and magnesium-deprived chromium cakes were subjected to a process of thermal decomposition at 650°C under oxygen-free conditions to reduce the amount of this waste and to study the effect of magnesium removal on the resulting products...
December 8, 2016: Waste Management & Research
https://www.readbyqxmd.com/read/27877077/amelioration-of-estrogen-deficiency-induced-obesity-by-collagen-hydrolysate
#2
Tsay-I Chiang, I-Chang Chang, Hsueh-Hui Lee, Kuang Hui Hsieh, Yung-Wei Chiu, Te-Jen Lai, Jer-Yuh Liu, Li-Sung Hsu, Shao-Hsuan Kao
Objectives: Menopausal transition with declining estrogen levels significantly affects the physiological properties of women and consequently contributes to a series of medical conditions, including obesity. Obesity is a crucial risk factor associated with cardiovascular diseases, diabetes mellitus, and breast cancer. Increasing dietary protein content improves satiety and energy expenditure. Thus, we hypothesize that supplementing with collagen, a common dietary protein, may alleviate menopause-induced obesity...
2016: International Journal of Medical Sciences
https://www.readbyqxmd.com/read/27832513/collagen-hydrolysates-of-skin-shavings-prepared-by-enzymatic-hydrolysis-as-a-natural-flocculant-and-their-flocculating-property
#3
Ruijie Fu, Kai Yao, Qisheng Zhang, Dongying Jia, Jiayuan Zhao, Yuanlong Chi
A series of collagen hydrolysates (CHs) were prepared from pigskin shavings by using pepsin (PCH), trypsin (TCH), Alcalase (ACH), HCl (HCH), and NaOH (NCH). Their physicochemical properties, including degree of collagen hydrolysis, molecular weight distribution, electric charge, and microstructure, were investigated, and their flocculation performance was evaluated in a kaolin suspension, at varied pHs and concentrations. PCH exhibited high flocculation capability under acidic and neutral conditions, and its efficiency for removing suspended particles was approximately 80% at a concentration of 0...
November 10, 2016: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/27768951/protective-effect-of-gelatin-peptides-from-pacific-cod-skin-against-photoaging-by-inhibiting-the-expression-of-mmps-via-mapk-signaling-pathway
#4
Tiejun Chen, Hu Hou, Yan Fan, Shikai Wang, Qianru Chen, Leilei Si, Bafang Li
Chronic exposure to ultraviolet (UV) irradiation causes skin photoaging. This study was undertaken to identify the anti-photoaging mechanisms of gelatin hydrolysate (CH) derived from pacific cod skin. Quantitative real-time reverse transcription-polymerase chain reaction (qRT-PCR) and ELISA assays were used to investigate the effects of CH on matrix metalloproteinases (MMPs) and the signaling pathways after UV irradiation by using a mice skin photoaging model. The average molecular weight of CH was 1200Da, and 273/1000 residues were hydrophobic, Gly-Pro and Gly-Leu sequences and Arg at C-terminus appeared frequently in CH...
October 14, 2016: Journal of Photochemistry and Photobiology. B, Biology
https://www.readbyqxmd.com/read/27763502/purification-of-antioxidant-peptides-by-high-resolution-mass-spectrometry-from-simulated-gastrointestinal-digestion-hydrolysates-of-alaska-pollock-theragra-chalcogramma-skin-collagen
#5
Liping Sun, Weidan Chang, Qingyu Ma, Yongliang Zhuang
In this study, the stable collagen hydrolysate was prepared by alcalase hydrolysis and twice simulated gastrointestinal digestion from Alaska pollock skin. The characteristics of hydrolysates and antioxidant activities in vitro, including 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) radical (ABTS(•+)) scavenging activity, ferric-reducing antioxidant power (FRAP) and hydroxyl radical (OH·) scavenging activity, were determined. After twice simulated gastrointestinal digestion of skin collagen (SGI-2), the degree of hydrolysis (DH) reached 26...
October 17, 2016: Marine Drugs
https://www.readbyqxmd.com/read/27747904/improvement-of-skin-condition-by-oral-administration-of-collagen-hydrolysates-in-chronologically-aged-mice
#6
Zhenbin Wang, Qing Wang, Lin Wang, Weidong Xu, Yuanqing He, Yunliang Li, Song He, Haile Ma
BACKGROUND: Collagen hydrolysates (CHs) has been demonstrated to have positive effect on skin photo-aging by topical application or oral ingestion. However, there's little report about its influence on skin chronological aging so far. RESULTS: Nine-month-old female ICR mice were given normal AIN-93M diets containing the CHs (2.5%, 5% and 10%, respectively) from Nile tilapia scale. After 6 months, the collagen content and antioxidant enzymes (superoxide dismutase and glutathione peroxidase) activities significantly increased (p < 0...
October 17, 2016: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/27640091/dipeptidyl-peptidase-ii-from-probiotic-pediococcus-acidilactici-purification-and-functional-characterization
#7
Dimpi Gandhi, Preeti Chanalia, Pooja Attri, Suman Dhanda
Dipeptidylpeptidase-II (DPP-II, E.C. 3.4.14.2), an exopeptidase was purified 15.4 fold with specific activity and yield of 15.4U/mg/mL and 14.68% respectively by a simple two step procedure from a probiotic Pediococcus acidilactici. DPP-II is 38.7KDa homodimeric serine peptidase with involvement of His and subunit mass of 18.9KDa. The enzyme exhibited optimal activity at pH 7.0 and 37°C with activation energy of 24.97kJ/mol. The enzyme retained more than 90% activity upto 50°C thus adding industrial importance...
September 14, 2016: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/27554764/the-preventive-effects-of-two-nutraceuticals-on-experimentally-induced-acute-synovitis
#8
E van de Water, M Oosterlinck, M Dumoulin, N M Korthagen, P R van Weeren, J van den Broek, H Everts, F Pille, D A van Doorn
BACKGROUND: Nutraceuticals are often used in the management of equine osteoarthritis, but scientific evidence of their efficacy is lacking. OBJECTIVES: To study the preventive effects of two new nutraceuticals after the experimental induction of synovitis in comparison with positive and negative control treatments. STUDY DESIGN: Blinded, controlled, randomised experiment. METHODS: Twenty-four healthy Standardbred horses were randomly allocated to supplement AT (multi-ingredient, 28 days), supplement HP (collagen hydrolysate, 60 days), meloxicam (4 days) or placebo (60 days)...
August 24, 2016: Equine Veterinary Journal
https://www.readbyqxmd.com/read/27553280/the-collagen-derived-dipeptide-hydroxyprolyl-glycine-promotes-c2c12-myoblast-differentiation-and-myotube-hypertrophy
#9
Tomoya Kitakaze, Tomotaka Sakamoto, Takehiro Kitano, Naoki Inoue, Fumihito Sugihara, Naoki Harada, Ryoichi Yamaji
The majority of studies on possible roles for collagen hydrolysates in human health have focused on their effects on bone and skin. Hydroxyprolyl-glycine (Hyp-Gly) was recently identified as a novel collagen hydrolysate-derived dipeptide in human blood. However, any possible health benefits of Hyp-Gly remain unclear. Here, we report the effects of Hyp-Gly on differentiation and hypertrophy of murine skeletal muscle C2C12 cells. Hyp-Gly increased the fusion index, the myotube size, and the expression of the myotube-specific myosin heavy chain (MyHC) and tropomyosin structural proteins...
September 23, 2016: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/27540823/oral-administration-of-collagen-hydrolysates-improves-glucose-tolerance-in-normal-mice-through-glp-1-dependent-and-glp-1-independent-mechanisms
#10
Yoshinori Iba, Koji Yokoi, Itsuka Eitoku, Masaki Goto, Seiko Koizumi, Fumihito Sugihara, Hiroshi Oyama, Tadashi Yoshimoto
The aim of this study was to evaluate the antidiabetic properties of collagen hydrolysates (CHs). CHs exhibited dipeptidyl peptidase-IV inhibitory activity and stimulated glucagon-like-peptide-1 (GLP-1) secretion in vitro. We also determined whether CHs improve glucose tolerance in normal mice. Oral administration of CHs suppressed the glycemic response during the oral and intraperitoneal glucose tolerance tests (OGTT and IPGTT), but the effects were weaker in IPGTT than in OGTT. CHs had no effect on the gastric emptying rate...
September 2016: Journal of Medicinal Food
https://www.readbyqxmd.com/read/27491029/protective-effect-of-gelatin-polypeptides-from-pacific-cod-gadus-macrocephalus-against-uv-irradiation-induced-damages-by-inhibiting-inflammation-and-improving-transforming-growth-factor-%C3%AE-smad-signaling-pathway
#11
Tiejun Chen, Hu Hou
Exposure to ultraviolet (UV) radiation results in skin damage, collagen reduction in the dermis, and consequently, premature skin aging (photoaging). The goal of this study was to examine the effect of gelatin hydrolysate (CH) from pacific cod (Gadus macrocephalus) skin on UV irradiation-induced inflammation and collagen reduction of photoaging mouse skin. The effect of CH on the activities of endogenous antioxidant enzymes was investigated. The expressions of nuclear factor-κB (NF-κB), proinflammatory cytokines, type I and type III procollagen, transforming growth factor-β1 (TGF-β1), type II receptor of TGF-β1 (TGF-βRII), and Smad7 were determined using quantitative real-time reverse transcription-polymerase chain reaction (qRT-PCR) and ELISA...
September 2016: Journal of Photochemistry and Photobiology. B, Biology
https://www.readbyqxmd.com/read/27384561/determination-of-free-form-and-peptide-bound-pyrraline-in-the-commercial-drinks-enriched-with-different-protein-hydrolysates
#12
Zhili Liang, Lin Li, Haiping Qi, Xia Zhang, Zhenbo Xu, Bing Li
Pyrraline, a causative factor for the recent epidemics of diabetes and cardiovascular disease, is also employed as an indicator to evaluate heat damage and formation of advanced glycation end-products (AGEs) in foods. Peptide-enriched drinks (PEDs) are broadly consumed worldwide due to rapid rate of absorption and perceived health effects. It can be hypothesized that PED is an important source of pyrraline, especially peptide bound pyrraline (Pep-Pyr). In this study we determined free-form pyrraline (Free-Pyr) and Pep-Pyr in drinks enriched with whey protein hydrolysate (WPH), soy protein hydrolysate (SPH) and collagen protein hydrolysate (CPH)...
2016: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/27283698/chicken-collagen-hydrolysate-cryoprotection-of-natural-actomyosin-mechanism-studies-during-freeze-thaw-cycles-and-simulated-digestion
#13
Lihui Du, Mirko Betti
The cryoprotective effect of chicken collagen hydrolysate (CCH) obtained from chicken skin was investigated at 0%, 4%, 8% and 12% (w/w) on natural actomyosin (NAM) model system to elucidate the possible mechanism. Ice dimensions in the NAM dispersions were measured after 7 thermal cycles (stabilized at -20°C and cycled between -16°C to -12°C) using a polarized microscope, demonstrating a significant reduction of ice crystal size induced by CCH. To determine the ice-controlling effect of CCH on protein freeze-denaturation, NAM samples were subjected to 7 freeze-thaw cycles between -20°C and 4°C...
November 15, 2016: Food Chemistry
https://www.readbyqxmd.com/read/27282460/effects-of-collagen-hydrolysate-on-the-tibialis-anterior-muscle-and-femur-in-senescence-accelerated-mouse-prone-6
#14
T Okiura, Y Oishi, A Takemura, A Ishihara
OBJECTIVES: Effects of collagen hydrolysate (CHD) on the oxidative capacity of the tibialis anterior muscle and the cortical and trabecular density of the femur were investigated in senescence-accelerated mouse prone 6 (SAMP6). METHODS: Sixteen-week-old male SAMP6 mice were divided into control (CON) and CHD groups. The CON group was given normal water, while the CHD group was given water containing CHD. Fibre cross-sectional areas (CSAs), fibre succinate dehydrogenase (SDH) staining intensity, and SDH activity of the tibialis anterior muscle were determined at 42 and 60 weeks of age...
June 1, 2016: Journal of Musculoskeletal & Neuronal Interactions
https://www.readbyqxmd.com/read/27185300/type-i-collagen-and-its-daughter-peptides-for-targeting-mucosal-healing-in-ulcerative-colitis-a-new-treatment-strategy
#15
Satiesh Kumar Ramadass, Sugin Lal Jabaris, Ramesh Kannan Perumal, Villianur Ibrahim HairulIslam, Arun Gopinath, Balaraman Madhan
Ulcerative colitis, particularly the chronic persistent form is characterized by the presence of active inflammation and extensive areas of ulceration in the colonic mucosa. The existing treatment protocol aims at only reducing intestinal inflammation, rather than targeting mucosal ulceration. In this study, type I collagen and its daughter peptides called collagen hydrolysate, highly popular reconstructive materials for tissue engineering applications, are hypothesized as healing matrices to target the recuperation of internal mucosal ulceration...
August 25, 2016: European Journal of Pharmaceutical Sciences
https://www.readbyqxmd.com/read/27177842/a-two-step-method-using-air-plasma-and-carbodiimide-crosslinking-to-enhance-the-biocompatibility-of-polycaprolactone
#16
Paramaporn Sahapaibounkit, Isarawut Prasertsung, Rattachat Mongkolnavin, Chiow San Wong, Siriporn Damrongsakkul
In this study, polycaprolactone (PCL) film, a high potential material used in biomedical applications, was treated by air plasma prior to a conjugation by carbodiimide cross-linking with various types of proteins, including type A gelatin, type B gelatin, and collagen hydrolysate. The properties of modified PCL films were characterized by X-ray photoelectron spectroscopy (XPS), contact angle measurement, and atomic force microscopy. The XPS results showed that oxygen and nitrogen atoms were successfully introduced on the air plasma-treated PCL surface...
May 14, 2016: Journal of Biomedical Materials Research. Part B, Applied Biomaterials
https://www.readbyqxmd.com/read/27162402/identification-of-bioactive-peptide-from-oreochromis-niloticus-skin-gelatin
#17
Sadabpong Choonpicharn, Suriya Tateing, Sanchai Jaturasitha, Nuansri Rakariyatham, Nuttee Suree, Hataichanoke Niamsup
Fish skin, one type of wastes generated from Nile tilapia processing, is still a good source of collagen and gelatin. Bioactive peptides can be obtained from Nile tilapia skin gelatin by trypsin digestion. Trypsin hydrolysate was subsequently purified by gel filtration chromatography. Trypsin A fraction showed the greatest reducing power (5.138 ± 1.060 μM trolox/mg peptide) among all hydrolysate fractions, while trypsin B fraction from gel filtration column was found to exhibit the best radical scavenging and angiotensin-I-converting enzyme (ACE) inhibitory activities 8...
February 2016: Journal of Food Science and Technology
https://www.readbyqxmd.com/read/27110500/study-of-hmg-coa-reductase-inhibition-activity-of-the-hydrolyzed-product-of-snakehead-fish-channa-striata-skin-collagen-with-50-kda-collagenase-from-bacillus-licheniformis-f11-4
#18
Agnes Virginia, Heni Rachmawati, Catur Riani, Debbie S Retnoningrum
Bioactive peptides produced from enzymatic hydrolysis fibrous protein have been proven to have several biological activities. Previous study showed that the hydrolysis product of snakehead fish skin collagen with 26 kDa collagenase from Bacillus licheniformis F11.4 showed HMG-CoA (HMGR) inhibition activity. The aim of this research was to determine the ability of the hydrolysis product produced from snakehead fish skin collagen hydrolysed by 50 kDa collagenase from B. licheniformis F11.4 in inhibiting HMGR activity...
January 2016: Scientia Pharmaceutica
https://www.readbyqxmd.com/read/27038545/collagencin-an-antibacterial-peptide-from-fish-collagen-activity-structure-and-interaction-dynamics-with-membrane
#19
Nadia Ennaas, Riadh Hammami, Ahmed Gomaa, François Bédard, Éric Biron, Muriel Subirade, Lucie Beaulieu, Ismail Fliss
In this study, we first report characterization of collagencin, an antimicrobial peptide identified from fish collagen hydrolysate. The peptide completely inhibited the growth of Staphylococcus aureus at 1.88 mM. Although non-toxic up to 470 μM, collagencin was hemolytic at higher concentrations. The secondary structure of collagencin was mainly composed by β-sheet and β-turn as determined by CD measurements and molecular dynamics. The peptide is likely to form β-sheet structure under hydrophobic environments and interacts with both anionic (phosphatidylglycerol) and zwitterionic (phosphoethanolamine and phosphatidylcholine) lipids as shown with CD spectroscopy and molecular dynamics...
April 29, 2016: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/26978646/efficient-absorption-of-x-hydroxyproline-hyp-gly-after-oral-administration-of-a-novel-gelatin-hydrolysate-prepared-using-ginger-protease
#20
Yuki Taga, Masashi Kusubata, Kiyoko Ogawa-Goto, Shunji Hattori
Recent studies have reported that oral intake of gelatin hydrolysate has various beneficial effects, such as reduction of joint pain and lowering of blood sugar levels. In this study, we produced a novel gelatin hydrolysate using a cysteine-type ginger protease having unique substrate specificity with preferential peptide cleavage with Pro at the P2 position. Substantial amounts of X-hydroxyproline (Hyp)-Gly-type tripeptides were generated up to 2.5% (w/w) concomitantly with Gly-Pro-Y-type tripeptides (5%; w/w) using ginger powder...
April 13, 2016: Journal of Agricultural and Food Chemistry
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