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Hepatic ischemia reperfusion

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https://www.readbyqxmd.com/read/29661451/ischemia-with-preconditioning-in-wistar-rats-maintains-mitochondrial-respiration-even-with-mild-hepatocellular-disturbance
#1
R R Tártaro, G L Jorge, A H Dianin, C A F Escanhoela, I F S F Boin
INTRODUCTION: In hepatectomy or liver transplantation, preconditioning is a procedure indicated to protect the organ from ischemia-reperfusion injury (I-R). OBJECTIVE: Evaluate the effect of preconditioning after hepatic I-R in Wistar rats, through mitochondrial respiration, liver histology, and profile. METHOD: Twenty male Wistar rats, weighing on average 307.1 g, were anesthetized with sodium thiopental (25 mg/kg) intravenously and xylazine hydrochloride (30 mg/kg) intramuscularly...
April 2018: Transplantation Proceedings
https://www.readbyqxmd.com/read/29661450/effect-of-hepatic-preconditioning-with-the-use-of-methylene-blue-on-the-liver-of-wistar-rats-submitted-to-ischemia-and-reperfusion
#2
T N de Almeida, J P Victorino, J Bistafa Liu, D Tófoli Queiroz Campos, C Graf, M C Jordani, L A Carneiro d'Albuquerque, K D S Mendes, O Castro-E-Silva
BACKGROUND: The liver may be injured in situations where it is submitted to ischemia, such as partial hepatectomy and liver transplantation. In all cases, ischemia is followed by reperfusion and, although it is essential for the reestablishment of tissue function, reperfusion may cause greater damage than ischemia, an injury characterized as ischemia-reperfusion (I/R) damage. The aim of this work was to analyze the effect of ischemic preconditioning with the use of methylene blue (MB; 15 mg/kg) 5 or 15 minutes before I/R (IRMB5' and IRMB15', respectively) on the hepatic injury occurring after I/R...
April 2018: Transplantation Proceedings
https://www.readbyqxmd.com/read/29621784/exosomes-derived-from-dendritic-cells-attenuate-liver-injury-by-modulating-the-balance-of-treg-and-th17-cells-after-ischemia-reperfusion
#3
Lei Zheng, Zhi Li, Wei Ling, Deming Zhu, Zhiwen Feng, Lianbao Kong
BACKGROUND/AIMS: The present study aimed to evaluate the effects as well as the underlying mechanisms of bone marrow-derived dendritic cells (BMDCs) and exosomes produced by BMDCs (DEXs) on hepatic ischemia-reperfusion (I/R) injury (IRI). METHODS: Primary hepatocytes were isolated and used to mimic the liver IR microenvironment. BMDCs were induced and characterized both biochemically with a flow cytometer (FCM) and biophysically with a microscope. Then, we exposed BMDCs to the supernatants from primary hepatocytes and evaluated the maturation of BMDCs by FCM...
March 29, 2018: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/29621129/plants-and-surgery-the-protective-effects-of-thymoquinone-on-hepatic-injury-a-systematic-review-of-in-vivo-studies
#4
REVIEW
Aysun Tekbas, Jutta Huebner, Utz Settmacher, Uta Dahmen
Multimodal treatment concepts including liver transplantation for hepatocellular carcinoma (HCC), extended resection methods and neoadjuvant chemotherapy for colorectal liver metastasis significantly improve patients’ outcome. However, surgery-induced hepatic ischemia-reperfusion injury (IRI) and chemotherapy-associated hepatotoxicity result in hepatocellular damage and compromised liver function. Activation of common key pathways in ischemic liver and hepatotoxic injury results in oxidative stress, inflammatory responses and apoptosis causing organ damage...
April 5, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29618155/von-willebrand-factor-aggravates-hepatic-ischemia-reperfusion-injury-by-promoting-neutrophil-recruitment-in-mice
#5
Yasuyuki Urisono, Asuka Sakata, Hideto Matsui, Shogo Kasuda, Shiro Ono, Kiyomi Yoshimoto, Kenji Nishio, Masayuki Sho, Masashi Akiyama, Toshiyuki Miyata, Kazuo Okuchi, Satoshi Nishimura, Mitsuhiko Sugimoto
Hepatic ischaemia-reperfusion (I/R) injury is a serious liver damage that critically influences the clinical outcome of liver surgery or transplantation. Since recent studies indicated the critical involvement of von Willebrand factor (VWF) in reperfusion injuries of brain and myocardium, we hypothesized that VWF-dependent thrombotic or inflammatory responses also play a role in hepatic I/R injury. Using a mouse model of hepatic I/R injury, we explored the functional relevance of the VWF-ADAMTS13 (a disintegrin and metalloproteinase with a thrombospondin type 1 motif, member 13) axis in this pathologic condition...
April 2018: Thrombosis and Haemostasis
https://www.readbyqxmd.com/read/29603199/indirubin-and-nad-prevent-mitochondrial-ischemia-reperfusion-damage-in-fatty-livers
#6
João Soeiro Teodoro, Ana Teresa Varela, Filipe Valente Duarte, Ana Patrícia Gomes, Carlos Marques Palmeira, Anabela Pinto Rolo
BACKGROUND: Fatty livers are considerably more susceptible to acute stressors, such as ischemia/reperfusion (I/R). Since the incidence of I/R is high due to surgical events and some pathologies, there is an urgent need to find strategies against I/R injury (I/RI) in fatty livers. We postulate that an acute pre-treatment with Indirubin-3'-oxime (Ind) or NAD+ prevents mitochondrial dysfunction associated with warm I/RI in fatty livers. MATERIALS AND METHODS: Zucker fatty rats were subjected to warm ischemia and 12 hrs of reperfusion...
March 30, 2018: European Journal of Clinical Investigation
https://www.readbyqxmd.com/read/29600900/bilirubin-acts-as-a-multi-potent-guardian-of-cardiovascular-integrity-more-than-just-a-radical-idea
#7
Andrew Cameron Bulmer, Bhavisha A Bakrania, Eugene Francois Du Toit, Ai-Ching Boon, Paul J Clark, Lawrie W Powell, Karl-Heinz Wagner, John P Headrick
Bilirubin, a potentially toxic catabolite of heme and indicator of hepato-biliary insufficiency, exhibits potent cardiac and vascular protective properties. Individuals with Gilbert's syndrome (GS) may experience hyperbilirubinemia in response to stressors including reduced hepatic bilirubin excretion/increased red blood cell breakdown, with individuals usually informed by their clinician that their condition is of little consequence. However, GS appears to protect from all-cause mortality, with progressively elevated total bilirubin associated with protection from ischemic heart and chronic obstructive pulmonary diseases...
March 30, 2018: American Journal of Physiology. Heart and Circulatory Physiology
https://www.readbyqxmd.com/read/29593814/age-related-differences-in-function-and-structure-of-rat-livers-subjected-to-ischemia-reperfusion
#8
Małgorzata Trocha, Anna Merwid-Ląd, Małgorzata Pieśniewska, Joanna Kwiatkowska, Lidia Fereniec-Gołębiewska, Przemysław Kowalski, Adam Szeląg, Tomasz Sozański
Introduction: Liver function is affected during ischemia/reperfusion (IR). The current state of knowledge about liver aging processes during IR is incomplete. We evaluated the effects of aging on liver structure and function under IR conditions. Material and methods: Animals were divided into control (C-2) and ischemia/reperfusion (IR-2) groups of young rats (2-4 months old) and C-12 and IR-12 groups of old rats (12-14 months old). The livers from IR-2 and IR-12 groups were subjected to partial ischemia (60 min), followed by global reperfusion (4 h)...
March 2018: Archives of Medical Science: AMS
https://www.readbyqxmd.com/read/29589772/alda-1-an-aldh2-activator-protects-against-hepatic-ischemia-reperfusion-injury-in-rats-via-inhibition-of-oxidative-stress
#9
Tao Zhang, Qiang Zhao, Fang Ye, Chan-Yan Huang, Wan-Mei Chen, Wen-Qi Huang
AIM: Previous studies have proved that activation of aldehyde dehydrogenase two (ALDH2) can attenuate oxidative stress through clearance of cytotoxic aldehydes, and can protect against cardiac, cerebral and lung ischemia/reperfusion (I/R) injuries. In this study, we investigated the effects of the ALDH2 activator Alda-1 on hepatic I/R injury. METHODS: Partial warm ischemia was performed in the left and middle hepatic lobes of Sprague-Dawley rats for 1 h, followed by 6 h of reperfusion...
March 28, 2018: Free Radical Research
https://www.readbyqxmd.com/read/29579874/cold-ischemia-time-as-a-factor-in-post-transplantation-complications-for-orthotopic-hepatic-transplantation
#10
Z Ruiz de Azúa-López, J R Naranjo-Izurieta, J Lameirao, L Martín-Villén, M Porras-López, N Palomo-Lopez, A Vilchez-Arenas, J J Egea-Guerrero
OBJECTIVE: This study aims to compare a shorter cold ischemia time with the present one in relation to the complications developed in liver transplantations. DESIGN: This is a retrospective, observational study of orthotopic liver transplantations performed with grafts from brain-dead donors during 12 months at a University Hospital (Seville). We compare incidence rates of complications (reperfusion syndrome, arterial and biliary complications, and prostaglandin requirements) between two groups according to cold ischemia times (group A < 6 hours; group B > 6 hours)...
March 2018: Transplantation Proceedings
https://www.readbyqxmd.com/read/29578092/quercetin-dose-affects-the-fate-of-hepatic-ischemia-and-reperfusion-injury-in-rats-an-experimental-research
#11
Mustafa Ufuk Uylaş, Adnan Şahin, Varol Şahintürk, İbrahim Özkan Alataş
BACKGROUND: Quercetin found in fruits and vegetables has an antioxidative effect. We aimed to investigate the protective effects of quercetin according to different doses on hepatic and ischemia-reperfusion (I/R) injury. METHODS: Fifty mature male Sprague-Dawley rats were randomly divided into five groups (n = 10 for each). All the animal groups underwent laparotomy. Group 1 rats served as a sham-operated group. Groups 2-5 underwent 1 h hepatic ischemia and were followed by 2 h reperfusion...
March 22, 2018: International Journal of Surgery
https://www.readbyqxmd.com/read/29569343/relaxing-liver-ischemia-reperfusion-injury-down-1-notch
#12
EDITORIAL
James F Markmann
Hepatic tissue damage due to ischemia-reperfusion (IR) is an important factor in liver transplant outcome and effective therapeutic interventions that mitigate IR injury have the potential to both improve early liver allograft function and to render more organs suitable for transplantation. Although a variety of approaches have shown promise in experimental transplant models, there have not been any that have successfully transitioned to widespread clinical application. In this context, the work of Kageyama et al (1), showing impressive attenuation of liver IR-damage after activation of Notch1 signaling pathway following treatment with an investigational drug serelaxin (SER; RLX030), is of major interest and potential importance from a clinical development perspective...
March 22, 2018: American Journal of Transplantation
https://www.readbyqxmd.com/read/29558716/protective-effects-of-phycocyanin-on-ischemia-reperfusion-liver-injuries
#13
Neyla Ben Gdara, Amel Belgacem, Ikram Khemiri, Safa Mannai, Lotfi Bitri
In this study, phycocyanin (Pc) extracted from Spirulina platensis was used to evaluate its antioxidants effects after ischemia/reperfusion injury (IRI) using the ex-vivo model of isolated perfused rat liver. The rats were divided into eight groups : Control group, where livers were directly perfused after their removal; Cold Ischemia group (CI), livers were treated in the same way as the control group, except that after their collection, they were stored for 12 h and 24 h in the Krebs Henseleit (KH) preservation solution at 4 °C and Treated group (PHY), livers were preserved in the same way as the preceding group except that the KH solution was enriched with phycocyanin at two different concentrations...
March 17, 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/29553556/purification-of-hepatocytes-and-sinusoidal-endothelial-cells-from-mouse-liver-perfusion
#14
Fatima Cabral, Colton M Miller, Katrina M Kudrna, Blake E Hass, Jocelyn G Daubendiek, Brianna M Kellar, Edward N Harris
This protocol demonstrates a method for obtaining high yield and viability for mouse hepatocytes and sinusoidal endothelial cells (SECs) suitable for culturing or for obtaining cell lysates. In this protocol, the portal vein is used as the site for catheterization, rather than the vena cava, as this limits contamination of other possible cell types in the final liver preparation. No special instrumentation is required throughout the procedure. A water bath is used as a source of heat to maintain the temperature of all the buffers and solutions...
February 12, 2018: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/29550823/rictor-deficiency-aggravates-hepatic-ischemia-reperfusion-injury-in-mice-by-suppressing-autophagy-and-regulating-mapk-signaling
#15
Dongwei Xu, Jianjun Zhu, Seogsong Jeong, Dawei Li, Xiangwei Hua, Lifeng Huang, Jianjun Zhang, Yi Luo, Qiang Xia
BACKGROUND/AIMS: The role of Rictor in hepatic ischemia/reperfusion (I/R) injury remains unknown. Here, we comprehensively examined the role of Rictor in hepatic I/R injury. METHODS: We studied the expression of Rictor during hepatic I/R injury. The regulatory effects of Rictor on inflammatory responses, cytokine and chemokine release, apoptotic and anti-apoptotic responses, and autophagy induction during hepatic I/R injury were identified via the shRNA-mediated knockdown of Rictor...
March 10, 2018: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/29545887/role-of-mitofusin-2-in-the-protective-effect-of-breviscapine-against-hepatic-ischemia-reperfusion-injury-in-rats
#16
Zhen Bao, Weijun Chen, Fan Pan, Bo Peng, Jin Gong
The purpose of the present study was to investigate the effect of breviscapine injection on hepatic ischemia/reperfusion (I/R) injury in rats. To explore the relevance and discuss the underlying mechanism of mitofusin 2 (Mfn2) in hepatic I/R injury, 40 Sprague-Dawley male rats were randomly and equally divided into five groups (n=8 per group) as follows: Sham, I/R + normal saline 1 (NS1), I/R + breviscapine 1 (Bre1), I/R + NS2 and I/R + Bre2 groups. Groups 1 and 2 represented ischemia for 20 and 60 min, respectively...
April 2018: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/29534658/gene-expression-profile-of-toll-like-receptor-adaptor-interferon-regulatory-factor-cytokine-axis-during-liver-regeneration-after-partial-ischemia-reperfusion-injury
#17
Meysam Nasiri, Mohammad-Hossein Karimi, Negar Azarpira, Iraj Saadat
OBJECTIVES: Toll-like receptors and downstream signal transduction pathways play pivotal roles in induction of inflammation, which is crucial for liver injury and regeneration. MATERIALS AND METHODS: Using a mouse model of partial hepatic ischemia-reperfusion injury followed by a 28-day time course for liver repair and regeneration, we assessed gene expression levels for Toll-like receptors, myeloid differentiation primary response 88, TIR-domain-containing adapter-inducing interferon-β, nuclear factor κB, interferon regulatory factors, tumor necrosis factor-α, and interleukins 1β and 6 at days 1, 4, 7, 14, and 28 after reperfusion in liver and blood cells by quantitative polymerase chain reaction...
March 9, 2018: Experimental and Clinical Transplantation
https://www.readbyqxmd.com/read/29524517/heme-oxygenase-1-protects-liver-against-ischemia-reperfusion-injury-via-phosphoglycerate-mutase-family-member-5-mediated-mitochondrial-quality-control
#18
Jeong-Min Hong, Sun-Mee Lee
AIMS: Heme oxygenase-1 (HO-1), an endogenous cytoprotective enzyme, is reported that can be localized in mitochondria under stress, contributing to preserve mitochondrial function. Mitochondrial quality control (QC) is essential to cellular health and recovery linked with redox homeostasis. Recent studies reported that phosphoglycerate mutase family member (PGAM) 5, a mitochondria-resident phosphatase, plays critical role in mitochondrial homeostasis. Therefore, we aim to investigate cytoprotective mechanisms of HO-1 in I/R-induced hepatic injury focusing on mitochondrial QC associated with PGAM5 signaling...
March 7, 2018: Life Sciences
https://www.readbyqxmd.com/read/29511679/prostaglandin-e1-preconditioning-attenuates-liver-ischemia-reperfusion-injury-in-a-rat-model-of-extrahepatic-cholestasis
#19
Feng Xu, Xiaolin Liu, Chao Wang, Chaoliu Dai
The aim of this study is to explore the hepatoprotective effect of intraportal prostaglandin E1 (PGE1) on liver ischemia reperfusion (IR) injury using an extrahepatic cholestatic model, observing oxidative stress markers, proinflammatory factors, apoptotic marker proteins, and an adhesion molecule. The extrahepatic cholestatic model was induced by common bile duct ligation. After seven days, rats were subjected to ischemia by Pringle maneuver for 15 min, followed by 1, 6, or 24 h of reperfusion. Prostaglandin E1 (PGE group) or normal saline (NS group) was continuously infused from 15 min before liver ischemia to 1 h after reperfusion...
2018: BioMed Research International
https://www.readbyqxmd.com/read/29509572/porcine-isolated-liver-perfusion-for-the-study-of-ischemia-reperfusion-injury-a-systematic-review
#20
Francesca Maione, Nicholas Gilbo, Silvia Lazzaro, Peter Friend, Giovanni Camussi, Renato Romagnoli, Jacques Pirenne, Ina Jochmans, Diethard Monbaliu
BACKGROUND: Understanding ischemia reperfusion injury (IRI) is essential to further improve outcomes after liver transplantation (LT). Porcine isolated liver perfusion (ILP) is increasingly used to reproduce LT-associated IRI in a strictly controlled environment. However, whether ILP is a reliable substitute of LT was never validated. METHODS: We systematically reviewed the current experimental set-ups for ILP and parameters of interest reflecting IRI. RESULTS: ILP was never compared to transplantation in animals...
March 5, 2018: Transplantation
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