keyword
https://read.qxmd.com/read/38510689/high-intensity-exercise-impairs-intestinal-barrier-function-by-generating-oxidative-stress
#21
JOURNAL ARTICLE
Maki Takami, Wataru Aoi, Karin Matsumoto, Yoji Kato, Yukiko Kobayashi, Masashi Kuwahata
The intestine functions as a barrier preventing the entry of extrinsic factors into the body. This barrier function is disrupted by oxidative damage along with an impaired mucosal layer. Excessive exercise can generate oxidative stress in the intestinal tissue; however, the effect of exercise-induced oxidative stress on intestinal permeability is unclear. In this study, we examined the involvement of oxidative stress in barrier function of the ileum of mice following high-intensity exercise. Male ICR mice (12-week-old) were divided into sedentary and exercise groups...
March 2024: Journal of Clinical Biochemistry and Nutrition
https://read.qxmd.com/read/38504643/effects-of-dietary-protein-level-on-intestinal-function-and-inflammation-in-nursery-pigs
#22
JOURNAL ARTICLE
Sarah C Pearce, Mitchell J Nisley, Brian J Kerr, Chris Sparks, Nicholas K Gabler
High crude protein (CP; 21-26%) diets fed during the first 21 to 28 d post-weaning are viewed negatively because of a perceived increase in the incidence rates of diarrhea due to increased intestinal protein fermentation and/or augmented enteric pathogen burden. This is thought to antagonize nursery pig health and growth performance. Therefore, our objective was to evaluate the impact of low vs. high dietary CP on 21-day post-weaned pig intestinal function. Analyzed parameters included ex vivo intestinal barrier integrity (ileum and colon), ileal nutrient transport, tissue inflammation, and fecal DM...
March 20, 2024: Journal of Animal Science
https://read.qxmd.com/read/38499842/gnt-v-mediated-aberrant-n-glycosylation-of-timp-1-promotes-diabetic-retinopathy-progression
#23
JOURNAL ARTICLE
Xiaoting Xi, Yanni Yang, Qianbo Chen, Jia Ma, Xuewei Wang, Yachun Deng, Xi Wang, Yan Li
BACKGROUND: Vascular endothelial growth factor (VEGF) signaling pathway plays an important role in the progression of diabetic retinopathy (DR). The glycosylation modification process of many key functional proteins in DR patients is abnormal. However, the potential involvement of abnormal N-glycoproteins in DR progression remains unclear. METHODS: Glycoproteomic profiling of the vitreous humor was performed. The level of protein and N-glycoprotein was confirmed by Western blot and Lectin blot, respectively...
March 18, 2024: Molecular Biology Reports
https://read.qxmd.com/read/38494905/quantification-of-rifampicin-loaded-into-inhaled-polymeric-nanoparticles-by-reversed-phase-high-performance-liquid-chromatography-in-pulmonary-nonphagocytic-cellular-uptake
#24
JOURNAL ARTICLE
Scolari Ivana Romina, De La Cruz-Thea Benjamín, Musri Melina Mara, Granero Gladys Ester
Rifampicin is an antibiotic effective against both Gram-negative and Gram-positive bacteria and is commonly used as a first-line treatment for tuberculosis caused by Mycobacterium tuberculosis . In this study, a reversed-phase high-performance liquid chromatography method was developed and validated to assess rifampicin, either free or combined with ascorbic acid, loaded into chitosan/Tween 80-coated alginate nanoparticles. The method utilized a reversed-phase C18 Restek column with specific chromatographic conditions: a mobile phase of 60 : 40 ratios of methanol/buffer phosphate (pH 7...
March 18, 2024: Analytical Methods: Advancing Methods and Applications
https://read.qxmd.com/read/38491103/role-of-gut-derived-bacterial-lipopolysaccharide-and-peripheral-tlr4-in-immobilization-stress-induced-itch-aggravation-in-a-mouse-model-of-atopic-dermatitis
#25
JOURNAL ARTICLE
Da-Eun Cho, Joon-Pyo Hong, Yoongeun Kim, Ju Yeon Sim, Heenam Stanley Kim, Song-Rae Kim, Bombi Lee, Hyo-Sung Cho, Ik-Hyun Cho, Sooan Shin, Mijung Yeom, Soon-Kyeong Kwon, In-Seon Lee, Hijoon Park, Kyuseok Kim, Dae-Hyun Hahm
Psychological stress and intestinal leakage are key factors in atopic dermatitis (AD) recurrence and exacerbation. Here, we demonstrate the mechanism underlying bacterial translocation across intestinal epithelial barrier damaged due to stress and further aggravation of trimellitic anhydride (TMA)-induced itch, which remain unclear, in AD mice. Immobilization (IMO) stress exacerbated scratching bouts and colon histological damage, and increased serum corticosterone and lipopolysaccharide (LPS). Orally administered fluorescein isothiocyanate (FITC)-dextran and surgically injected (into the colon) Cy5...
March 15, 2024: Scientific Reports
https://read.qxmd.com/read/38490394/pharmacokinetics-tissue-distribution-and-subacute-toxicity-of-oral-carrageenan-in-mice
#26
JOURNAL ARTICLE
Jiahui Wang, Kehan Zhu, Miaomiao Zhang, Qian Zhou, Wen Ji, Zhen Yao, Duxin Li
Carrageenan (CGN) is a typical sulfated polysaccharide widely applied in the food and pharmaceutical industries. Its in vivo behavior plays vital roles in understanding structural and biological functional relationships. The lack of UV chromophores in highly sulfated polysaccharides presents a challenge for their in vivo behavior studies. Therefore, this study aimed to develop a fast and effective quantitative fluorescence method for investigating the pharmacokinetics and tissue distribution of CGN. Fluorescence isothiocyanate labeling of CGN (FCGN) and microplate reader-based measurements were developed and validated to study its pharmacokinetics...
March 13, 2024: International Journal of Biological Macromolecules
https://read.qxmd.com/read/38488442/erucin-a-natural-isothiocyanate-exerts-pro-angiogenic-properties-in-cultured-endothelial-cells-and-reverts-angiogenic-impairment-induced-by-high-glucose
#27
JOURNAL ARTICLE
Shirley Genah, Valerio Ciccone, Arianna Filippelli, Vittoria Simonis, Alma Martelli, Eugenia Piragine, Eleonora Pagnotta, Nicola Pecchioni, Vincenzo Calderone, Lucia Morbidelli
Insufficient vessel maintenance adversely impacts patients in terms of tissue reperfusion following stroke or myocardial infarction, as well as during wound healing. Angiogenesis impairment is a feature typical of metabolic disorders acting at the cardiovascular level, such as diabetes. Therapeutic angiogenesis regulation offers promising clinical implications, and natural compounds as pro-angiogenic nutraceuticals hold valuable applications in regenerative medicine. By using cultured endothelial cells from human umbilical veins (HUVEC) we studied functional and molecular responses following exposure to erucin, a natural isothiocyanate derived from Brassicaceae plants and extracted from the seeds of rocket...
March 15, 2024: Phytotherapy Research: PTR
https://read.qxmd.com/read/38483252/killing-two-birds-with-one-stone-shape-memory-cryogels-for-hemostasis-and-wound-repair
#28
JOURNAL ARTICLE
Mengna Dong, Lin Luo, Kunyi He, Jinbo Cao, Zhenyu Li, Xiaogang Hu, Li Wang
The development of shape-memory hemostatic agents is crucial for the treatment of deep incompressible bleeding tissue. However, there are few reports on biomaterials that can monitor bacterial infection at the wound site in real time following hemostasis and effectively promote repair. In this study, we propose a multifunctional QCSG/FLZ cryogel composed of glycidyl methacrylate-functionalized quaternary chitosan (QCSG), fluorescein isothiocyanate (FITC), and a lysozyme (LYZ)-modified zeolitic imidazolate framework (ZIF-8) for incompressible bleeding tissue hemostasis and wound repair...
March 14, 2024: ACS Applied Materials & Interfaces
https://read.qxmd.com/read/38481530/cyclodextrin-derivatives-decrease-transient-receptor-potential-vanilloid-1-and-ankyrin-1-ion-channel-activation-via-altering-the-surrounding-membrane-microenvironment-by-cholesterol-depletion
#29
JOURNAL ARTICLE
Andrea Kinga Nehr-Majoros, János Erostyák, Éva Fenyvesi, Edina Szabó-Meleg, Levente Szőcs, György Sétáló, Zsuzsanna Helyes, Éva Szőke
Transient Receptor Potential Vanilloid 1 (TRPV1) and Ankyrin 1 (TRPA1) are nonselective cation channels expressed in primary sensory neurons and several other non-neuronal structures such as immune cells, keratinocytes, and vascular smooth muscle cells. They play important roles in nociception, pain processing and their chanellopathies are associated with the development of several pathological conditions. They are located in cholesterol- and sphingolipid-rich membrane lipid raft regions serving as platforms to modulate their activations...
2024: Frontiers in Cell and Developmental Biology
https://read.qxmd.com/read/38479670/study-of-chondrogenesis-of-umbilical-cord-mesenchymal-stem-cells-in-curdlan-poly-vinyl-alcohol-composite-hydrogels-and-its-mechanical-properties-of-freezing-thawing-treatments
#30
JOURNAL ARTICLE
Yu-Chieh Su, Yu Chang, Wei-Chang Lee, Jhih-Han Wang, Takumi Narita, Hiroyuki Takeno, Jie-Yu Syu, I-Ming Jou, Wen-Chuan Hsieh
The curdlan gel is a natural material produced by bacteria. It utilizes chemical cross-linking reactions to form a 3D porous composite hydrogel, increasing its porosity and water content, and improving its mechanical properties. It can be used in tissue repair and regenerative medicine. Curdlan-Poly(vinyl alcohol) (PVA) composite hydrogel can rapidly swell within 1 min due to its porous structure. Compression tests confirmed that it still maintains its original mechanical strength, even after five repeated freeze-thaw (FT) processes, making it suitable for long-term cryopreservation...
March 11, 2024: International Journal of Biological Macromolecules
https://read.qxmd.com/read/38479137/the-potential-for-reducing-aflatoxin-b1-contamination-of-stored-peanuts-by-soil-disinfection
#31
JOURNAL ARTICLE
Anmin Guo, Yi Zhang, Yutong Ji, Xinhua Chen, Wei Zhang, Xuemei Liu, Dongdong Yan, Wensheng Fang, Yuan Li, Aocheng Cao, Qiuxia Wang
Aflatoxins from the fungus Aspergillus flavus (A. flavus) that contaminate stored peanuts is a major hazard to human health worldwide. Reducing A. flavus in soil can decrease the risk of aflatoxins in stored peanuts. In this experiment, we determined whether peanuts grown on soil fumigated with dazomet (DZ), metham sodium (MS), allyl isothiocyanate (AITC), chloropicrin (PIC) or dimethyl disulfide (DMDS) would reduce of the quantity of A. flavus and its toxin's presence. The results of bioassays and field tests showed that PIC was the most effective fumigant for preventing and controlling A...
February 28, 2024: Journal of Hazardous Materials
https://read.qxmd.com/read/38473991/isothiocyanates-potentiate-tazemetostat-induced-apoptosis-by-modulating-the-expression-of-apoptotic-genes-members-of-polycomb-repressive-complex-2-and-levels-of-tri-methylating-lysine-27-at-histone-3-in-human-malignant-melanoma-cells
#32
JOURNAL ARTICLE
Ioannis Anestopoulos, Ioannis Paraskevaidis, Sotiris Kyriakou, Lambrini E Giova, Dimitrios T Trafalis, Sotiris Botaitis, Rodrigo Franco, Aglaia Pappa, Mihalis I Panayiotidis
In this study, we utilized an in vitro model consisting of human malignant melanoma as well as non-tumorigenic immortalized keratinocyte cells with the aim of characterizing the therapeutic effectiveness of the clinical epigenetic drug Tazemetostat alone or in combination with various isothiocyanates. In doing so, we assessed markers of cell viability, apoptotic induction, and expression levels of key proteins capable of mediating the therapeutic response. Our data indicated, for the first time, that Tazemetostat caused a significant decrease in viability levels of malignant melanoma cells in a dose- and time-dependent manner via the induction of apoptosis, while non-malignant keratinocytes were more resistant...
February 27, 2024: International Journal of Molecular Sciences
https://read.qxmd.com/read/38472870/broccoli-amaranth-and-red-beet-microgreen-juices-the-influence-of-cold-pressing-on-the-phytochemical-composition-and-the-antioxidant-and-sensory-properties
#33
JOURNAL ARTICLE
Spasoje D Belošević, Danijel D Milinčić, Uroš M Gašić, Aleksandar Ž Kostić, Ana S Salević-Jelić, Jovana M Marković, Verica B Đorđević, Steva M Lević, Mirjana B Pešić, Viktor A Nedović
The aim of this study was to analyze in detail the phytochemical composition of amaranth (AMJ), red beet (RBJ), and broccoli (BCJ) microgreens and cold-pressed juices and to evaluate the antioxidant and sensory properties of the juices. The results showed the presence of various phenolic compounds in all samples, namely betalains in amaranth and red beet microgreens, while glucosinolates were only detected in broccoli microgreens. Phenolic acids and derivatives dominated in amaranth and broccoli microgreens, while apigenin C -glycosides were most abundant in red beet microgreens...
February 29, 2024: Foods (Basel, Switzerland)
https://read.qxmd.com/read/38469233/a-facs-based-novel-isolation-technique-identifies-heterogeneous-ctcs-in-oral-squamous-cell-carcinoma
#34
JOURNAL ARTICLE
Anshika Chauhan, Arnab Pal, Meenakshi Sachdeva, Geeta S Boora, Monil Parsana, Jaimanti Bakshi, Roshan Kumar Verma, Radhika Srinivasan, Debajyoti Chatterjee, Arindam Maitra, Sushmita Ghoshal
PURPOSE: Isolating circulating tumour cells (CTCs) from the blood is challenging due to their low abundance and heterogeneity. Limitations of conventional CTC detection methods highlight the need for improved strategies to detect and isolate CTCs. Currently, the Food and Drug Administration (FDA)-approved CellSearch™ and other RUO techniques are not available in India. Therefore, we wanted to develop a flexible CTC detection/isolation technique that addresses the limitation(s) of currently available techniques and is suitable for various downstream applications...
2024: Frontiers in Oncology
https://read.qxmd.com/read/38467571/synthesis-of-some-novel-thiophene-analogues-as-potential-anticancer-agents
#35
JOURNAL ARTICLE
Altaf S Almatari, Ali Saeed, Ghada E Abdel-Ghani, Mahmood M S Abdullah, Hamad A Al-Lohedan, Ehab Abdel-Latif, Amr El-Demerdash
The aim of this study involves the synthesis novel thiophene analogues that can be used as anticancer medications through a strategic multicomponent reaction connecting ethyl 4-chloroacetoacetate (1), phenyl isothiocyanate, and a series of active methylene reagents, including ethyl acetoacetate (2), malononitrile, ethyl cyanoacetate, cyanoacetamide 6a-c, N-phenyl cyanoacetamide derivatives 13a-c, and acetoacetanilide derivatives 18. This reaction was facilitated by dry dimethylformamide with a catalytic quantity of K2CO3...
March 11, 2024: Chemistry & Biodiversity
https://read.qxmd.com/read/38465235/establishment-and-application-of-a-rapid-molecular-diagnostic-platform-for-the-isothermal-visual-amplification-of-group-b-streptococcus-based-on-recombinase-polymerase
#36
JOURNAL ARTICLE
Meilin Liu, Huan Wang, Chu Chu, Fanli Min, Lizhou Sun, Teng Zhang, Qian Meng
With growing concerns about Group B streptococcal (GBS) infections and their adverse effects on perinatal pregnancies, including infection, premature delivery, neonatal septicemia, and meningitis, it is urgent to promote GBS screening at all pregnancy stages. The purpose of this study is to establish a device-independent, fast, sensitive, and visual GBS detection method. Taking advantage of the characteristics of the recombinase polymerase isothermal amplification (RPA), the activity of the nfo nuclease cleavage base analog (tetrahydrofuran, THF) site, and the advantages of visual reading of the lateral flow chromatography strip (LFS), a GBS detection method was developed...
2024: Frontiers in Cellular and Infection Microbiology
https://read.qxmd.com/read/38450092/mass-trapping-of-the-diamondback-moth-plutella-xylostella-l-by-a-combination-of-its-sex-pheromone-and-allyl-isothiocyanate-in-cabbage-fields-in-southern-vietnam
#37
JOURNAL ARTICLE
Dinh Thi Chi, Ho Le Thi, Le Van Vang, Tran Thanh Thy, Masanobu Yamamoto, Tetsu Ando
A lure composed of ( Z )-11-hexadecenal, ( Z )-11-hexadecenyl acetate, and ( Z )-11-hexadecen-1-ol at a ratio of 5 : 5 : 1 at a dose of 0.01 mg was optimal for the attraction of the Vietnamese strain of the diamondback moth (DBM). The combination of the sex pheromone with a plant volatile, allyl isothiocyanate, significantly increased the attraction of the pheromone trap. Females were also attracted, but they were only about 2% of all moths captured. In plots with 120-130 traps per ha, mass trapping with the combined lures reduced the DBM larval densities in cabbage fields as effectively as the spraying of insecticides 6 to 8 times...
February 20, 2024: Journal of Pesticide Science
https://read.qxmd.com/read/38447617/artemisia-capillaris-thunb-polysaccharide-alleviates-cholestatic-liver-injury-through-gut-microbiota-modulation-and-nrf2-signaling-pathway-activation-in-mice
#38
JOURNAL ARTICLE
Jingyi Cai, Zhenyun Zhu, Yuanyuan Li, Qi Li, Tian Tian, Qian Meng, Tianming Wang, Yueming Ma, Jiasheng Wu
ETHNOPHARMACOLOGICAL RELEVANCE: According to traditional Chinese medicine (TCM) theory, cholestasis belongs to category of jaundice. Artemisia capillaris Thunb. has been widely used for the treatment of jaundice in TCM. The polysaccharides are the one of main active components of the herb, but its effects on cholestasis remain unclear. AIM OF THE STUDY: To investigate the protective effect and mechanism of Artemisia capillaris Thunb. polysaccharide (APS) on cholestasis and liver injury...
March 4, 2024: Journal of Ethnopharmacology
https://read.qxmd.com/read/38447056/a-comparative-analysis-of-git-barrier-function-and-immune-markers-in-gilt-vs-sow-progeny-at-birth-and-weaning
#39
JOURNAL ARTICLE
Udani A Wijesiriwardana, John R Pluske, Jessica R Craig, John B Furness, Mitchell Ringuet, Linda J Fothergill, Frank R Dunshea, Jeremy J Cottrell
Progeny born to primiparous sows (gilt progeny; GP) have lower birth, weaning and slaughter weights than sow progeny (SP). GP also have reduced gastrointestinal tract (GIT) development, as evidenced by lower organ weights. Therefore, the aim of this experiment was to quantify changes in GIT barrier function that occur birth and weaning, representing two major challenges to the young piglet. The effects of parity (GP vs SP) in GIT barrier integrity function was quantified at four timepoints: birth (~0 h), 24 hours after birth (24 h), 1-day pre-weaning (PrW) and 1-day post-weaning (PoW) in commercially reared piglets...
March 6, 2024: Journal of Animal Science
https://read.qxmd.com/read/38443552/improvement-in-the-quality-and-productivity-of-codonopsis-pilosula-seedlings-by-dazomet-soil-fumigation
#40
JOURNAL ARTICLE
Hongyan Wang, Yuan Chen, Fengxia Guo, Pengbin Dong, Wei Liang, Jiali Cheng
Dazomet is a dry powder formulation that releases toxic gas containing methyl isothiocyanate, which controls soil-borne pests and weeds, improving crop yields when applied to moist soils. To explore the efficacy of dazomet fumigation in the cultivation of the perennial herb Codonopsis pilosula, four typical cultivars (G1, G2, W1 and TCK) in Gansu Province were selected for seedling cultivation after soil fumigation (F) by dazomet, and non-fumigated soil was used as a control (CK). The experiments took 2 years to complete...
March 5, 2024: Scientific Reports
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