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https://www.readbyqxmd.com/read/28231170/fermentation-assisted-extraction-of-isothiocyanates-from-brassica-vegetable-using-box-behnken-experimental-design
#1
Amit K Jaiswal, Nissreen Abu-Ghannam
Recent studies showed that Brassica vegetables are rich in numerous health-promoting compounds such as carotenoids, polyphenols, flavonoids, and glucosinolates (GLS), as well as isothiocyanates (ITCs) and are involved in health promotion upon consumption. ITCs are breakdown products of GLS, and typically used in the food industry as a food preservative and colouring agent. They are also used in the pharmaceutical industry due to their several pharmacological properties such as antibacterial, antifungal, antiprotozoal, anti-inflammatory, and chemoprotective effects, etc...
November 4, 2016: Foods (Basel, Switzerland)
https://www.readbyqxmd.com/read/28230606/secretions-of-bifidobacterium-infantis-and-lactobacillus-acidophilus-protect-intestinal-epithelial-barrier-function
#2
Shuangshuang Guo, Tyler Gillingham, Yuming Guo, Di Meng, Weishu Zhu, W Allen Walker, Kriston Ganguli
OBJECTIVES: The secreted metabolites of probiotics are cytoprotective to intestinal epithelium and have been shown to attenuate inflammation and reduce gut permeability. The present study was designed to determine the protective effects of probiotic conditioned media (PCM) from Bifidobacterium infantis (BCM) and Lactobacillus acidophilus (LCM) on interleukin (IL)-1β-induced intestinal barrier compromise. METHODS: The epithelial barrier was determined by measuring the transepithelial electrical resistance (TER) across a Caco-2 cell monolayer using a Transwell model...
March 2017: Journal of Pediatric Gastroenterology and Nutrition
https://www.readbyqxmd.com/read/28224893/influence-of-molecular-weight-and-structure-on-diffusion-rates-and-mechanisms-of-dextran-polyelectrolyte-complexes
#3
Yasunori Miyazaki, Midori Mieno, Tomonobu Uchino, Yoshiyuki Kagawa
The release profiles of fluorescein isothiocyanate-dextran (FD) at various molecular weights (20-500kDa) and the swelling behaviors of polyelectrolyte complex-forming tablets were investigated. Four types of tablets were prepared using different combinations of the following chemicals: carboxymethyl dextran (CMD), dextran sulfate (DS), 2-diethylaminoethyl dextran (EA), and 2-hydroxypropyltrimethylammonium dextran (CDC). Release tests were conducted via the rotating basket method at pH 6.8. Swelling behaviors were analyzed via the front movement method...
April 15, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/28220706/human-cannabinoid-receptor-2-ligand-interaction-motif-transmembrane-helix-2-cysteine-c2-59-89-as-determinant-of-classical-cannabinoid-agonist-activity-and-binding-pose
#4
Han Zhou, Yan Peng, Aneetha Halikhedkar, Pusheng Fan, David R Janero, Ganesh A Thakur, Richard Warren Mercier, Xin Sun, Xiaoyu Ma, Alexandros Makriyannis
Cannabinoid receptor 2 (CB2R)-dependent signaling is implicated in neuronal physiology and immune surveillance by brain microglia. Selective CB2R agonists hold therapeutic promise for inflammatory and other neurological disorders. Information on human CB2R (hCB2R) ligand-binding and functional domains is needed to inform the rational design and optimization of candidate drug-like hCB2R agonists. Prior demonstration that hCB2R transmembrane helix 2 (TMH2) cysteine C2.59(89) reacts with small-molecule methanethiosulfonates showed that this cysteine residue is accessible to sulfhydryl derivatization reagents...
February 21, 2017: ACS Chemical Neuroscience
https://www.readbyqxmd.com/read/28208275/comprehensive-three-dimensional-gas-chromatography-with-time-of-flight-mass-spectrometry
#5
Nathanial E Watson, H Daniel Bahaghighat, Ke Cui, Robert E Synovec
Development of comprehensive, three-dimensional (3D) gas chromatography with time-of-flight mass spectrometric detection (GC(3)/TOFMS) is described. This instrument provides four dimensions (4D) of chemical selectivity and includes significant improvements to total selectivity (mass spectrometric and chromatographic), peak identification, and operational temperature range relative to previous models of the GC(3) reported. The new instrumental design and data output are evaluated and illustrated via two samples, a 115-component test mixture and a diesel fuel spiked with several compounds, for the purpose of illustrating the chemical selectivity benefits of this instrumental platform...
February 7, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28203057/kelussia-odoratissima-mozaff-activates-intrinsic-pathway-of-apoptosis-in-breast-cancer-cells-associated-with-s-phase-cell-cycle-arrest-via-involvement-of-p21-p27-in-vitro-and-in-vivo
#6
Hamed Karimian, Aditya Arya, Mehran Fadaeinasab, Mahboubeh Razavi, Maryam Hajrezaei, Ataul Karim Khan, Hapipah Mohd Ali, Mahmood Ameen Abdulla, Mohamad Ibrahim Noordin
BACKGROUND: The aim of this study was to evaluate the anticancer potential of Kelussia odoratissima. Several in vitro and in vivo biological assays were applied to explore the direct effect of an extract and bioactive compound of this plant against breast cancer cells and its possible mechanism of action. MATERIALS AND METHODS: K. odoratissima methanol extract (KME) was prepared, and MTT assay was used to evaluate the cytotoxicity. To identify the cytotoxic compound, a bioassay-guided investigation was performed on methanol extract...
2017: Drug Design, Development and Therapy
https://www.readbyqxmd.com/read/28192141/glossoscolex-paulistus-hemoglobin-with-fluorescein-isothiocyanate-steady-state-and-time-resolved-fluorescence
#7
Ana E B Barros, Marina B Barioni, Francisco A O Carvalho, Amando Siuiti Ito, Marcel Tabak
Glossoscolex paulistus extracellular hemoglobin (HbGp) stability has been followed, in the presence of urea, using fluorescein isothiocyanate (FITC). Binding of FITC to HbGp results in a significant quenching of probe fluorescence. Tryptophan emission decays present four characteristic lifetimes: two in the sub-nanosecond/picosecond, and two in the nanosecond time ranges. Tryptophan decays for pure HbGp and HbGp-FITC systems are similar. In the absence of denaturant, and up to 2.5mol/L of urea, the shorter lifetimes predominate...
February 10, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28191982/adsorption-of-amino-acids-and-glutamic-acid-based-surfactants-on-imogolite-clays
#8
Massimo Bonini, Alessio Gabbani, Stefano Del Buffa, Francesca Ridi, Piero Baglioni, Romain Bordes, Krister Holmberg
Aluminum oxide surfaces are of utmost interest in different biotech applications, in particular for their use as adjuvants (i.e., booster of the immune response against infectious agents in vaccines production). In this framework, imogolite clays combine the chemical flexibility of an exposed alumina surface with 1D nanostructure. This work reports on the interaction between amino acids and imogolite, using turbidimetry, ζ-potential measurements, and Fourier transform infrared spectroscopy as main characterization tools...
February 22, 2017: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/28186438/a-semiautomated-microfluidic-platform-for-real-time-investigation-of-nanoparticles-cellular-uptake-and-cancer-cells-tracking
#9
Mariana R Carvalho, F Raquel Maia, Joana Silva-Correia, Bruno M Costa, Rui L Reis, Joaquim M Oliveira
AIM: Develop a platform composed of labeled dendrimer nanoparticles (NPs) and a microfluidic device for real-time monitoring of cancer cells fate. MATERIALS & METHODS: Carboxymethylchitosan/poly(amidoamine) dendrimer NPs were labeled with fluorescein-5(6)-isothiocyanate and characterized using different physicochemical techniques. After, HeLa, HCT-116 and U87MG were cultured in the presence of NPs, and cell viability and internalization efficiency in static (standard culture) and dynamic (microfluidic culture) conditions were investigated...
February 10, 2017: Nanomedicine
https://www.readbyqxmd.com/read/28182427/artifact-free-and-detection-profile-independent-higher-order-fluorescence-correlation-spectroscopy-for-microsecond-resolved-kinetics-2-mixtures-and-reactions
#10
Farshad Abdollah-Nia, Martin P Gelfand, Alan K Van Orden
Fluorescence correlation spectroscopy (FCS) is a primary tool in the time-resolved analysis of non-reacting or reacting molecules in solution, based on fluorescence intensity fluctuations. However, conventional FCS alone is insufficient for complete determination of reaction or mixture parameters. In an accompanying article, a technique for computation of artifact-free higher-order correlations with microsecond time resolution was described. Here, we demonstrate applications of the technique to analyze systems of fast and slow reactions...
February 9, 2017: Journal of Physical Chemistry. B
https://www.readbyqxmd.com/read/28182406/spiro-cyclopropanes-from-intramolecular-cyclopropanation-of-pyranopyrazoles-and-pyranopyrimidine-diones-and-lewis-acid-mediated-3-2-cycloadditions-of-spirocyclopropylpyrazolones
#11
Prasun Mukherjee, Asish R Das
A robust intramolecular cyclopropanation reaction was first performed on pyrano-pyrazole and pyrano-pyrimidine dione derivatives to obtain spiro-cyclopropyl pyrazolones and barbiturates, using iodosylbenzene (PhIO) or the combination of iodobenzene diacetate (PIDA)/ molecular iodine (I2), under mild reaction conditions. Syntheses of functionally and stereochemically diversified, novel spiropyrazolone fused 2-iminothiophene and spiropyrazolone fused pyrroline scaffolds were also demonstrated via Lewis acid catalyzed highly diastereoselective (3+2) cycloaddition reactions of the synthesized spiro-cyclopropyl pyrazolones with phenyl isothiocyanate and benzonitrile respectively...
February 9, 2017: Journal of Organic Chemistry
https://www.readbyqxmd.com/read/28182113/evaluation-of-an-aqueous-extract-from-horseradish-root-armoracia-rusticana-radix-against-lipopolysaccharide-induced-cellular-inflammation-reaction
#12
Corinna Herz, Hoai Thi Thu Tran, Melinda-Rita Márton, Ronald Maul, Susanne Baldermann, Monika Schreiner, Evelyn Lamy
Horseradish (Armoracia rusticana) is a perennial crop and its root is used in condiments. Traditionally, horseradish root is used to treat bacterial infections of the respiratory tract and urinary bladder. The antiphlogistic activity, determined in activated primary human peripheral blood mononuclear cells (PBMC), was evaluated for an aqueous extract and its subfractions, separated by HPLC. Compound analysis was done by UHPLC-QToF/MS and GC-MS. The aqueous extract concentration-dependently inhibited the anti-inflammatory response to lipopolysaccharide (LPS) in terms of TNF-α release at ≥37 μg/mL...
2017: Evidence-based Complementary and Alternative Medicine: ECAM
https://www.readbyqxmd.com/read/28176286/aptamer-combined-with-fluorescent-silica-nanoparticles-for-detection-of-hepatoma-cells
#13
Zixi Hu, Juntao Tan, Zongqiang Lai, Rong Zheng, Jianhong Zhong, Yiwei Wang, Xiaoxue Li, Nuo Yang, Jieping Li, Wei Yang, Yong Huang, Yongxiang Zhao, Xiaoling Lu
PURPOSE: The purpose of this study is to develop a simple, effective method to label hepatoma cells with aptamers and then detect them using fluorescent silica nanoparticles (FSNPs). METHOD: Streptavidin was conjugated to carboxyl-modified fluorescein isothiocyanate (FITC)-doped silica nanoparticles which were prepared by the reverse microemulsion method. The resulting streptavidin-conjugated fluorescent silica nanoparticles (SA-FSNPs) were mixed with hepatoma cells that had been labeled with biotin-conjugated aptamer TLS11a (Bio-TLS11a)...
December 2017: Nanoscale Research Letters
https://www.readbyqxmd.com/read/28169240/synergistic-induction-of-apoptosis-in-b-cell-chronic-lymphocytic-leukemia-cells-after-treatment-with-all-trans-retinoic-acid-in-combination-with-interleukin-21-and-rituximab
#14
Kazem Abbaszadeh-Goudarzi, Fazel Shokri, Mostafa Hosseini, Farhad Jadidi-Niaragh, Ghasem Ghalamfarsa, Ali Akbar Saboor-Yaraghi
AIM: B-cell chronic lymphocytic leukemia (B-CLL) is the most common adult leukemia in the Western world and characterized by the progressive expansion of malignant B lymphocytes in peripheral blood. In spite of advances in sciences to recognize the number of effective agents for the treatment of chronic lymphocytic leukemia (CLL), this leukemia is thought as incurable one. Introducing a new therapy that has a direct effect on B-CLL lymphocytes and no cytotoxic effects on the other cells is a great wish...
October 2016: Journal of Cancer Research and Therapeutics
https://www.readbyqxmd.com/read/28165733/-2%C3%AF-2%C3%AF-cycloaddition-of-isocyanates-to-uranium-iv-imido-complexes-for-the-synthesis-of-u-iv-%C3%AE%C2%BA-2-ureato-compounds
#15
Caleb J Tatebe, Matthias Zeller, Suzanne C Bart
A new family of uranium(IV) imido complexes of the form Tp*2U(NR) (Tp* = hydrotris(3,5-dimethylpyrazolyl)borate; R = benzyl (Bn), para-tolyl (p-Tol), 2,6-diethylphenyl (detp), and 2,6-diisopropylphenyl (dipp)) have been generated by bibenzyl extrusion from Tp*2UBn. Tp*2U(NBn), Tp*2U(Np-Tol), and Tp*2U(Ndetp), along with previously reported Tp*2U(NPh) and Tp*2U(NAd) (Ad = 1-adamantyl), readily undergo [2π+2π] cycloaddition with isocyanates and isothiocyanates to generate κ(2)-ureato and κ(2)-thioureato derivatives, respectively...
February 20, 2017: Inorganic Chemistry
https://www.readbyqxmd.com/read/28165151/horseradish-extract-promotes-urinary-bladder-carcinogenesis-when-administered-to-f344-rats-in-drinking-water
#16
Young-Man Cho, Mai Hasumura, Toshio Imai, Shigeaki Takami, Akiyoshi Nishikawa, Kumiko Ogawa
Horseradish extract (HRE), consisting mainly of a mixture of allyl isothiocyanate and other isothiocyanates, has been used as a food additive. To evaluate the potential hazards of HRE, a 104-week chronic study, a 2-week analysis of cell proliferation in the urinary bladder and a medium-term promotion bioassay of HRE were conducted with administration at concentrations of up to 0.04% HRE in the drinking water to male F344 rats. In the 104-week chronic study with 32 male rats per group, no treatment-related increases in the incidences of neoplastic lesions in any organ, including urinary bladder, were observed, except for simple hyperplasia in the urinary bladder in rats treated with HRE at concentrations of more than 0...
February 6, 2017: Journal of Applied Toxicology: JAT
https://www.readbyqxmd.com/read/28162552/-314-meningeal-application-of-low-ph-il-6-and-allyl-isothiocyanate-produces-migraine-related-behavior-in-mice
#17
C Burgos-Vega, L Quigley, G Trevisan, T Price, G Dussor
No abstract text is available yet for this article.
April 2016: Journal of Pain: Official Journal of the American Pain Society
https://www.readbyqxmd.com/read/28160435/fast-analysis-of-domoic-acid-using-microchip-electrophoresis-with-laser-induced-fluorescence-detection
#18
Yongqiang Cheng, Cuilian Guo, Bin Zhao, Li Yang
A fast and effective method was developed to detect domoic acid based upon microchip electrophoresis combined with laser induced fluorescence detection. Through study of the gated injection process on the cross channel of microchip, low-voltage mode with relatively longer sample loading time was adopted to reduce the sample discrimination and improve the signal sensitivity. Fluorescein isothiocyanate was used as derivatizing reagent for domoic acid. Under the optimized conditions, domoic acid was completely separated in 60 s with separation efficiency of 1...
February 4, 2017: Journal of Separation Science
https://www.readbyqxmd.com/read/28157839/establishment-of-a-method-for-evaluating-endothelial-cell-injury-by-tnf-%C3%AE-in-vitro-for-clarifying-the-pathophysiology-of-virus-associated-acute-encephalopathy
#19
Kyohei Miyazaki, Koichi Hashimoto, Masatoki Sato, Masahiro Watanabe, Naoki Tomikawa, Shuto Kanno, Yukihiko Kawasaki, Nobuo Momoi, Mitsuaki Hosoya
BACKGROUND: Virus-associated acute encephalopathy (VAE) is a severe central nervous system complication caused by common viral infections in children. The pathophysiology of VAE is thought to be endothelial injury. This study was designed to establish an in vitro VAE model for evaluating endothelial injury caused by the proinflammatory cytokine TNF-α. METHODS: Transwell-grown human umbilical vein endothelial cells (HUVECs) monolayers were incubated with serially diluted TNF-α...
February 3, 2017: Pediatric Research
https://www.readbyqxmd.com/read/28154260/identification-of-adenovirus-derived-cell-penetrating-peptide
#20
Takamasa Hirai, Yoshiaki Yamagishi, Naoya Koizumi, Miwa Nonaka, Rina Mochida, Kenta Shida, Tetsuya Nomura, Makiko Fujii, Fuminori Sakurai, Hiroyuki Mizuguchi, Yoshiteru Watanabe, Naoki Utoguchi
Cell-penetrating peptides (CPPs) have been highly anticipated as an efficient delivery system due to their ability to cross biological membranes and transport various cargoes into cells. In the present study, we have identified adenovirus-derived CPPs using various capsid-mutant adenovirus (Ad) vectors. First, we examined the endocytosis-inducing ability of these vectors. A fiber-shaft substituted Ad vector, Ad type 5 vector with the fiber shaft domain replaced by that derived from Ad type 35, induced the highest fluorescein isothiocyanate (FITC)-dextran uptake into a human liver cell line, HepG2 cells...
2017: Biological & Pharmaceutical Bulletin
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