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Ryuichi Ohgaki, Yuji Teramura, Daichi Hayashi, Lili Quan, Suguru Okuda, Shushi Nagamori, Madoka Takai, Yoshikatsu Kanai
Various physiological and pathological processes are accompanied with the alteration of pH at extracellular juxtamembrane region. Accordingly, the methods to analyze the cell surface pH have been demanded in biological and medical sciences. In this study, we have established a novel methodology for cell surface pH imaging using poly(ethylene glycol)-phospholipid (PEG-lipid) as a core structure of ratiometric fluorescent probes. PEG-lipid is a synthetic amphiphilic polymer originally developed for the cell surface modification in transplantation therapy...
December 13, 2017: Scientific Reports
Azim Ziyaei Halimehjani, Martin Dračínský, Petr Beier
A one-pot three-component route for the synthesis of S-trifluoromethyl dithiocarbamates by the reaction of secondary amines, carbon disulfide and Togni's reagent is described. The reactions proceed in moderate to good yields. A similar reaction using a primary aliphatic amine afforded the corresponding isothiocyanate in high yield. A variable temperature NMR study revealed a rotational barrier of 14.6, 18.8, and 15.9 kcal/mol for the C-N bond in the dithiocarbamate moiety of piperidine, pyrrolidine, and diethylamine adducts, respectively...
2017: Beilstein Journal of Organic Chemistry
Su-Xia Ma, Zhi-Feng Bai, Wei Wang, Hui-Ying Wu
BACKGROUND: This study aims to investigate the regulative role of microRNA-93 (miR-93) in mouse cardiac microvascular endothelial cells (CMECs) injury and inflammatory response by negatively targeting SPP1 gene via the NF-κB signaling pathway. METHODS: Healthy Balb/c mice were recruited to establish mouse model with myocarditis using CVB3 virus. Mice were grouped into the normal, blank, negative control (NC), miR-93 inhibitor, miR-93 mimic, SPP1 shRNA and miR-93 mimic + SPP1 shRNA groups...
December 12, 2017: Journal of Cellular Biochemistry
M Soledade C Pedras, Q Huy To
The syntheses of perdeuterated phytoalexins nasturlexins A and C, and putative biosynthetic precursors, including phenylethyl isothiocyanates and phenylethyl dithiocarbamates, using commercially available [2,3,4,5,6-D5 ]phenylalanine, [2,3,4,5,6-D5 ]nitrobenzene and [2,3,4,5,6-D5 ]benzaldehyde are described. In addition, application of an efficient deuterium-hydrogen exchange transformation to non-labeled starting materials allowed access to new deuterated compounds, including 3-hydroxyphenylethyl glucosinolate...
December 12, 2017: Journal of Labelled Compounds & Radiopharmaceuticals
Yi-Fei Li, Jia-Sheng Wu, Yuan-Yuan Li, Yan Dai, Min Zheng, Jia-Kai Zeng, Guo-Feng Wang, Tian-Ming Wang, Wen-Kai Li, Xue-Yan Zhang, Ming Gu, Cheng Huang, Li Yang, Zheng-Tao Wang, Yue-Ming Ma
This study explored the effects of chicken bile powder (CBP), a 2000-year-old Chinese medicine, on α-naphthyl isothiocyanate (ANIT)-induced intrahepatic cholestasis in mice. CBP treatment for 14 days significantly ameliorated ANIT-induced changes in serum alanine aminotransferase, aspartate aminotransferase, bile acids, bilirubin, γ-glutamyl transpeptidase, alkaline phosphatase, and liver tissue morphology. Serum metabolomics showed changes in 24 metabolites in ANIT-exposed mice; 16 of these metabolites were reversed by CBP treatment via two main pathways (bile acid biosynthesis and arachidonic acid metabolism)...
November 14, 2017: Oncotarget
Pramod Upadhyaya, Adam T Zarth, Naomi Fujioka, Vincent A Fritz, Stephen S Hecht
Glucobrassicin, a quantitatively significant constituent of Brassica vegetables, gives rise to indole-3-carbinol (I3C) and its dimer di-indolylmethane (DIM) when the vegetables are chewed. I3C and DIM have been extensively studied with respect to their anti-carcinogenic properties. However, the presumed intermediate isothiocyanate in their formation, indole-3-methyl isothiocyanate (IMITC), has to our knowledge never been observed, despite the fact that isothiocyanates derived from cruciferous vegetables are known to have anti-carcinogenic properties...
December 2, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
Manli Feng, Pengfei Yang, Gaosheng Yang, Wenlong Chen, Zhuo Chai
The [3+2] annulations of γ-butyrolactone fused donor-acceptor (D-A) cyclopropanes with aromatic isothiocyanates and dialkyl carbodiimides promoted by FeCl3 is reported. A series of bicyclic/polycyclic γ-butyrolactone fused thioimidates and γ-butyrolactone fused amidines containing four contiguous stereogenic centers were obtained in excellent yields as single stereoisomers.
December 8, 2017: Journal of Organic Chemistry
Hui Chen, Chunxiao Wang, Moci Qi, Lihua Ge, Zhenchuan Tian, Jinhua Li, Min Zhang, Min Wang, Linfang Huang, Xiaofei Tang
Objective: To explore whether Rhaponticum uniflorum (R. uniflorum) had anti-tumor effects in oral cancer and investigate the molecular mechanisms involved in these anti-tumor effects. Methods: Chemical compositions of R. uniflorum ethyl acetate (RUEA) extracts were detected by ultra-performance liquid chromatography-Q/time-of-flight mass spectrometry (UPLC-Q/TOF-MS), followed by pharmacology-based network prediction analysis. The effects of RUEA extracts on proliferation, apoptosis, migration, and invasion ability of human oral squamous cell carcinoma (OSCC) cell line SCC15 were evaluated by CCK8 assay, Annexin V- fluorescein isothiocyanate/propidium iodide staining, wound healing assay, and Matrigel invasion assay, respectively...
2017: Frontiers in Pharmacology
Xuan-Yan Shi, Wei Ding, Tie-Qiu Li, Yi-Xiong Zhang, Shan-Chao Zhao
BACKGROUND Histone deacetylase (HDAC) inhibitors are emerging as a new class of anti-cancer drugs that promote cancer cell apoptosis, and include suberoylanilide hydroxamic acid (SAHA). The aim of this study was to investigate the mechanism of SAHA-induced apoptosis in human prostate cancer cell lines, DU145 and PC-3. MATERIAL AND METHODS Cell lines, DU145 and PC-3, were studied before and after treatment with SAHA. The effects of SAHA treatment on cell proliferation were studied using the MTT cell proliferation assay...
December 6, 2017: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
Verena Jeschke, Emily E Kearney, Katharina Schramm, Grit Kunert, Anton Shekhov, Jonathan Gershenzon, Daniel G Vassão
Multiple lepidopteran larvae feed successfully on plants containing glucosinolates despite the diverse array of toxic and deterrent breakdown products, such as isothiocyanates (ITCs), formed upon plant damage. While much is known about how specialist lepidopterans metabolize and tolerate glucosinolates, there is little information about the metabolic fate of these plant defense compounds in specialized herbivores. Employing 13C- and 14C-labeled 4-methylsulfinylbutyl glucosinolate (glucoraphanin), we identified and quantified the major detoxification products of glucosinolates and ITCs in selected specialized and generalist larvae...
2017: Frontiers in Plant Science
Noriyuki Mori, Manami Kurata, Hanae Yamazaki, Shigenobu Matsumura, Takashi Hashimoto, Kazuki Kanazawa, Tomonori Nadamoto, Kazuo Inoue, Tohru Fushiki
The transient receptor potential (TRP) V1 is a cation channel belonging to the TRP channel family and it has been reported to be involved in energy metabolism, especially glucose metabolism. While, we have previously shown that intragastric administration of allyl isothiocyanate (AITC) enhanced glucose metabolism via TRPV1, the underlying mechanism has not been elucidated. In this study, we examined the relationship between insulin secretion and the increase in carbohydrate oxidation due to AITC. Intragastric administration of AITC elevated blood insulin levels in mice and AITC directly enhanced insulin secretion from isolated islets...
December 6, 2017: Bioscience, Biotechnology, and Biochemistry
Zhiquan Li, Hui Dong, Mo Li, Yaoping Wu, Yanwu Liu, Yinan Zhao, Xiaochao Chen, Minliang Ma
Honokiol is the main active constituent of Magnolia officinalis. With effective and long‑term pharmacological functions of being antibacterial, anti‑oxidative, anti‑inflammatory, antitumor, anti‑spasmic, anti‑anxiety and anti‑viral, Honokiol is clinically used in the treatment of acute enteritis and chronic gastritis. The aim of the present study was to observe the possible anti‑effects of honokiol on autophagy and apoptosis of osteosarcoma, and to investigate the role of the PI3K/Akt/mTOR signaling pathway in its anticancer effects...
November 21, 2017: Molecular Medicine Reports
Dan Huang, Li Yin, Xinxin Liu, Bo Lv, Zulong Xie, Xuedong Wang, Bo Yu, Yao Zhang
Administration of bone marrow‑derived mesenchymal stem cells (MSCs) has emerged as a potential therapeutic approach for the treatment of myocardial infarction (MI). However, the increase in reactive oxygen species (ROS) in ischemic cardiac tissue compromises the survival of transplanted MSCs, thus resulting in limited therapeutic efficiency. Therefore, strategies that attenuate oxidative stress‑induced damage and enhance MSC viability are required. Geraniin has been reported to possess potent antioxidative activity and exert protective effects on numerous cell types under certain conditions...
November 21, 2017: International Journal of Molecular Medicine
M P Chrysouli, C N Banti, N Kourkoumelis, N Panayiotou, G S Markopoulos, A J Tasiopoulos, S K Hadjikakou
The antiproliferative activity of the gold complex [Au(tpp)Cl] (1) (tpp=triphenyphosphine) against human breast adenocarcinoma cells (MCF-7) and normal human fetal lung fibroblast cells (MRC-5) was investigated. The compound exhibits stronger activity against MCF-7 cells than cisplatin. The apoptotic pathway, especially though the mitochondrion damage was concluded by cell cycle arrest, flow cytometry using Annexin V-Fluorescein IsoThioCyanate (FITC) and Propidium Iodide (PI) as indicators, assays and permeabilization of the mitochondrial membrane tests...
November 8, 2017: Journal of Inorganic Biochemistry
Ho Yen Fong, Sri Nurestri Abd Malek, Hui Shin Yee, Saiful Anuar Karsani
Background: Cervical cancer has become one of the most common cancers in women and currently available treatment options for cervical cancer are very limited. Naturally occurring chalcones and its derivatives have been studied extensively as a potential anticancer agent in different types of cancer and helichrysetin is naturally occurring chalcone that possess potent antiproliferative activity toward human cancer cells. Materials and Methods: Inhibitory activity of helichrysetin was evaluated at different concentrations...
October 2017: Pharmacognosy Magazine
Kota Sekiguchi, Erina Ogawa, Kohta Kurohane, Hideyuki Konishi, Narumi Mochizuki, Kei Manabe, Yasuyuki Imai
Little attention has been paid to chemicals that can enhance hypersensitivity caused by other chemicals. We have demonstrated that phthalate esters with short chain alcohols enhance fluorescein isothiocyanate (FITC)-induced contact hypersensitivity (CHS) in a mouse model. Furthermore, phthalate esters with such an enhancing effect were found to activate transient receptor potential ankyrin 1 (TRPA1) cation channels, which are expressed on a part of sensory neurons, using a TRPA1-expressing cell line. In this study, we examined these activities of esters comprising glycerol and a short chain fatty acid, i...
November 30, 2017: Toxicology Letters
Nadiya T Darwish, Shamala D Sekaran, Yatimah Alias, Sook Mei Khor
The sharp increase in incidence of dengue infection has necessitated the development of methods for the rapid diagnosis of this deadly disease. Here we report the design and development of a reliable, sensitive, and specific optical immunosensor for the detection of the dengue nonstructural protein 1 (NS1) biomarker in clinical samples obtained during early stages of infection. The present optical NS1 immunosensor comprises a biosensing surface consisting of specific monoclonal NS1 antibody for immunofluorescence-based NS1 antigen determination using fluorescein isothiocyanate (FITC) conjugated to IgG antibody...
November 30, 2017: Journal of Pharmaceutical and Biomedical Analysis
Yuan Li, Jianan Ren, Xiuwen Wu, Jieshou Li
Some patients with intra-abdominal infection (IAI) may develop intra-abdominal hypertension (IAH) during treatment. This study investigated the impact of IAI combined with IAH on the intestinal mucosal barrier in a rabbit model. Forty-eight New Zealand white rabbits were randomly divided into four groups: (1) IAI & IAH; (2) IAI alone; (3) IAH alone; and (4) Control group. IAI model: cecal ligation and puncture for 48 hours; IAH model: raised intra-abdominal pressure of 20 mmHg for 4 hours. Pathological changes of intestinal mucosa were confirmed by light and scanning electron microscopy...
December 1, 2017: Bioscience Reports
Peter J Bruhn, Vahagn C Nikolian, Ihab Halaweish, Zhigang Chang, Martin Sillesen, Baoling Liu, Yongqing Li, Hasan B Alam
BACKGROUND: Microvascular hyperpermeability resulting from endothelial barrier dysfunction (EBD) is associated with worse clinical outcomes in trauma-induced hemorrhagic shock. We have previously shown that treatment with Tubastatin A (TubA), a histone deacetylase 6 inhibitor, improves outcomes in animal models of shock. In this study, we investigate whether TubA treatment may prevent trauma-related EBD. METHODS: Wistar-Kyoto rats subjected to 40% hemorrhage were treated with TubA or vehicle control...
November 21, 2017: Journal of Trauma and Acute Care Surgery
Labrini V Athanasiou, Maria C Kantere, Constantinos S Kyriakis, Dimitra Pardali, Katerina Adamama Moraitou, Zoe S Polizopoulou
Canine distemper is a common and potentially lethal multisystemic disease caused by the Canine distemper virus (CDV). We evaluated the diagnostic performance of direct immunofluorescent assay (FA) and cytology to detect CDV antigen in conjunctival cells compared with an established polymerase chain reaction (PCR) detection assay used as a gold standard for CDV diagnosis. Samples were collected from 57 young dogs presenting with central nervous system signs compatible with distemper disease. Exfoliative epithelial cells were collected from the right and left conjunctiva of each animal using nylon-bristled cytobrushes for cytology and cotton swabs for FA and PCR...
November 29, 2017: Viral Immunology
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