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https://www.readbyqxmd.com/read/28110507/computer-assisted-quantification-of-cd3-t-cells-in-follicular-lymphoma
#1
Fazly S Abas, Arwa Shana'ah, Beth Christian, Robert Hasserjian, Abner Louissaint, Michael Pennell, Berkman Sahiner, Weijie Chen, Muhammad Khalid Khan Niazi, Gerard Lozanski, Metin Gurcan
The advance of high resolution digital scans of pathology slides allowed development of computer based image analysis algorithms that may help pathologists in IHC stains quantification. While very promising, these methods require further refinement before they are implemented in routine clinical setting. Particularly critical is to evaluate algorithm performance in a setting similar to current clinical practice. In this article, we present a pilot study that evaluates the use of a computerized cell quantification method in the clinical estimation of CD3 positive (CD3+) T cells in follicular lymphoma (FL)...
January 22, 2017: Cytometry. Part A: the Journal of the International Society for Analytical Cytology
https://www.readbyqxmd.com/read/28109881/production-of-aba-responses-requires-both-the-nuclear-and-cytoplasmic-functional-involvement-of-pyr1
#2
EunJoo Park, Tae-Houn Kim
Abscisic acid (ABA) enhances stress tolerant responses in plants against unfavorable environmental conditions. In Arabidopsis, ABA promotes interactions between PYR/PYL/RCARs and PP2C, thereby allowing SnRK2s to phosphorylate downstream components required for the regulation of gene expression or for gating ion channels. Because PYR1 is known to localize to nucleus and cytoplasm it is a question whether nuclear or cytoplasmic PYR1 confer different functions to the ABA signaling pathway, as has been previously shown for regulatory proteins...
January 18, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28109618/effects-of-dmards-on-citrullinated-peptide-autoantibody-levels-in-ra-patients-a-longitudinal-analysis
#3
Carolin Wunderlich, Isabelle Oliviera, Camille P Figueiredo, Juergen Rech, Georg Schett
OBJECTIVE: To study whether stable treatment with DMARDs affects anti-CCP2 antibody levels in patients with rheumatoid arthritis. METHODS: In this longitudinal observational study 100 RA patients were followed for anti-CCP2 IgG antibody (U/L) and total IgG level (mg/dL) every 6 months for a total period of 2.5 years. All patients received stable DMARD treatment during this period. Five groups comprising each 20 patients were analyzed as follows: (1) methotrexate (MTX) alone, (2) tumor necrosis factor inhibitors (TNFi), (3) tocilizumab (TCZ), (4) rituximab (RTX), and (5) abatacept (ABA)...
October 3, 2016: Seminars in Arthritis and Rheumatism
https://www.readbyqxmd.com/read/28106309/transposable-elements-tes-contribute-to-stress-related-long-intergenic-noncoding-rnas-in-plants
#4
Dong Wang, Zhipeng Qu, Lan Yang, Qingzhu Zhang, Zhi-Hong Liu, Trung Do, David L Adelson, Zhen-Yu Wang, Iain Searle, Jian-Kang Zhu
Noncoding RNAs have been extensively described in plant and animal transcriptomes by using high-throughput sequencing technology. Of these non-coding RNAs, a growing number of long intergenic noncoding RNAs (lincRNAs) have been described in multicellular organisms, however the origins and functions of many lincRNAs remain to be explored. In many eukaryotic genomes, transposable elements (TEs) are widely distributed and often account for large fractions of plant and animal genomes yet the contribution of TEs to lincRNAs is largely unknown...
January 20, 2017: Plant Journal: for Cell and Molecular Biology
https://www.readbyqxmd.com/read/28106252/investigation-into-the-role-of-endogenous-abscisic-acid-during-ripening-of-imported-avocado-cv-hass
#5
Marjolaine D Meyer, Gemma A Chope, Leon A Terry
BACKGROUND: The importance of ethylene in avocado ripening has been extensively studied. In contrast, little is known about the possible role of abscisic acid (ABA). The present work studied the effect of 1-MCP (0.3 μL L(-1) ), e + (®) Ethylene Remover and the combination thereof on the quality of imported avocado cv. Hass fruit stored for 7 days at 12 °C. Ethylene production, respiration, firmness, colour, heptose (C7) sugars and ABA concentrations in mesocarp tissue were measured throughout storage...
January 20, 2017: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/28105733/vitamin-d-supplementation-for-sickle-cell-disease
#6
REVIEW
Htoo Htoo Kyaw Soe, Adinegara Bl Abas, Nan Nitra Than, Han Ni, Jaspal Singh, Abdul Razzak Bin Mohd Said, Ifeyinwa Osunkwo
BACKGROUND: Sickle cell disease is a genetic chronic haemolytic and pro-inflammatory disorder. The clinical manifestations of sickle cell disease result from the presence of mutations on the beta globin genes that generate an abnormal haemoglobin product (called haemoglobin S) within the red blood cell. Sickle cell disease can lead to many complications such as acute chest syndrome, stroke, acute and chronic bone complications (including painful vaso-occlusive crisis, osteomyelitis, osteonecrosis and osteoporosis)...
January 20, 2017: Cochrane Database of Systematic Reviews
https://www.readbyqxmd.com/read/28103799/comparative-rna-seq-based-transcriptomic-analysis-of-bud-dormancy-in-grape
#7
Muhammad Khalil-Ur-Rehman, Long Sun, Chun-Xia Li, Muhammad Faheem, Wu Wang, Jian-Min Tao
BACKGROUND: Bud dormancy is an important biological phenomenon of perennial plants that enables them to survive under harsh environmental circumstances. Grape (Vitis vinifera) is one of the most grown fruit crop worldwide; however, underlying mechanisms involved in grape bud dormancy are not yet clear. This work was aimed to explore the underlying molecular mechanism regulating bud dormancy in grape. RESULTS: We have performed transcriptome and differential transcript expression analyses of "Shine Muscat" grape buds using the Illumina RNA-seq system...
January 19, 2017: BMC Plant Biology
https://www.readbyqxmd.com/read/28101788/embling-production-in-althaea-officinalis-l-through-somatic-embryogenesis-and-their-appraisal-via-histological-and-scanning-electron-microscopical-studies
#8
Ruphi Naz, Mohammad Anis, Abdulrahman A Alatar
In vitro propagation of a medicinally important plant, Althaea officinalis, has been achieved through somatic embryogenesis. Somatic embryos (globular to torpedo-shaped embryos) were induced on Murashige and Skoog's (MS) medium augmented with various concentrations of 2,4-dichlorophenoxyacetic acid (2,4-D, 5.0, 10.0, 15.0, 20.0, and 25.0) alone or combined with N6-benzylaminopurine (BA, 0.1, 0.5, 1.0, 1.5, and 2.0 μM). These were directly formed from the cut ends and subsequently spread on the whole surface of internodal explants...
January 18, 2017: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/28101117/maternal-mortality-ratio-and-causes-of-death-in-iri-between-2009-and-2012
#9
Marzieh Vahiddastjerdy, Nasrin Changizi, Abas Habibollahi, Leila Janani, Zahra Farahani, Farah Babaee
Objective: The Maternal Mortality Ratio is an important health indicator. We presented the distribution and causes of maternal mortality in Islamic Republic of Iran. Materials and methods: After provision of an electronic Registry system for date entry, a descriptive-retrospective data collection had been performed for all maternal Deaths in March 2009- March 2012. All maternal deaths and their demographic characteristic were identified by using medical registries, death certificates, and relevant codes according to International Classification of Diseases (ICD-9) during pregnancy, labor, and 42 days after parturition...
September 2016: Journal of Family & Reproductive Health
https://www.readbyqxmd.com/read/28097691/oilseed-rape-nac56-transcription-factor-modulates-reactive-oxygen-species-accumulation-and-hypersensitive-response-like-cell-death
#10
Qinqin Chen, Fangfang Niu, Jingli Yan, Bisi Chen, Feifei Wu, Xiaohua Guo, Bo Yang, Yuan-Qing Jiang
The NAC (NAM, ATAF1/2, CUC2) transcription factor gene family is plant-specific and plays diverse roles in development and responses to abiotic stresses and pathogen challenge. Oilseed rape (Brassica napus) or canola is an important oil crop worldwide, however, the function of NAC genes in it remains largely elusive. Here, we identified and characterized the NAC56 gene isolated from oilseed rape. Expression of BnaNAC56 was induced by abscisic acid (ABA), jasmonic acid (JA), methyl viologen (MV) and a necrotrophic fungal pathogen Sclerotinia sclerotiorum, but repressed by cold...
January 18, 2017: Physiologia Plantarum
https://www.readbyqxmd.com/read/28095733/decoding-crucial-lncrnas-implicated-in-neurogenesis-and-neurological-disorders
#11
R Ayana, Shailja Singh, Soumya Pati
Unraveling transcriptional heterogeneity and the labyrinthine nature of neurodevelopment can probe insights into neuropsychiatric disorders. It is noteworthy that adult neurogenesis is restricted to the Sub Ventricular and Sub Granular zones (SVZ/SGZ) of the brain. Recent studies suggest long non-coding RNAs (lncRNAs) as an avant-garde class of regulators implicated in neurodevelopment. But, paucity exists in the knowledge regarding lncRNAs in neurogenesis and their associations with neurodevelopmental defects...
January 17, 2017: Stem Cells and Development
https://www.readbyqxmd.com/read/28095469/characterization-of-dof-transcription-factors-and-their-responses-to-osmotic-stress-in-poplar-populus-trichocarpa
#12
Han Wang, Shicheng Zhao, Yuchi Gao, Jingli Yang
The DNA-binding One Zinc Finger (Dof) genes are ubiquitous in many plant species and are especial transcription regulators that participate in plant growth, development and various procedures, including biotic and abiotic stress reactions. In this study, we identified 41 PtrDof members from Populus trichocarpa genomes and classified them into four groups. The conserved motifs and gene structures of some PtrDof genes belonging to the same subgroup were almost the same. The 41 PtrDof genes were dispersed on 18 of the 19 Populus chromosomes...
2017: PloS One
https://www.readbyqxmd.com/read/28093875/what-are-the-evolutionary-origins-of-stomatal-responses-to-abscisic-acid-aba-in-land-plants
#13
Frances C Sussmilch, Timothy J Brodribb, Scott A M McAdam
The evolution of active stomatal closure in response to leaf water deficit, mediated by the hormone abscisic acid (ABA), has been the subject of recent debate. Two different models for the timing of the evolution of this response recur in the literature. A single-step model for stomatal control suggests that stomata evolved active, ABA-mediated control of stomatal aperture, when these structures first appeared, prior to the divergence of bryophyte and vascular plant lineages. In contrast, a gradualistic model for stomatal control proposes that the most basal vascular plant stomata responded passively to changes in leaf water status...
January 17, 2017: Journal of Integrative Plant Biology
https://www.readbyqxmd.com/read/28089983/detailed-analysis-of-the-articular-domain-in-patients-with-primary-sj%C3%A3-gren-syndrome
#14
Rada V Moerman, Suzanne Arends, Petra M Meiners, Arjan Vissink, Frederik K L Spijkervet, Frans G M Kroese, Elisabeth Brouwer, Hendrika Bootsma
OBJECTIVE: We used the 28-joint Disease Activity Score (DAS28) and the European League Against Rheumatism Sjögren's Syndrome Disease Activity Index (ESSDAI) articular domain to assess the effect of rituximab (RTX) and abatacept (ABA) on articular involvement in primary Sjögren syndrome (pSS). METHODS: Patients with pSS treated with RTX (n = 18) or ABA (n = 13) and having a DAS28 erythrocyte sedimentation rate (ESR)/C-reactive protein (CRP) level ≥ 3.2 at baseline were selected...
January 15, 2017: Journal of Rheumatology
https://www.readbyqxmd.com/read/28074873/the-nac-type-transcription-factor-osnac2-regulates-aba-dependent-genes-and-abiotic-stress-tolerance-in-rice
#15
Jiabin Shen, Bo Lv, Liqiong Luo, Jianmei He, Chanjuan Mao, Dandan Xi, Feng Ming
Plants can perceive environmental changes and respond to external stressors. Here, we show that OsNAC2, a member of the NAC transcription factor family, was strongly induced by ABA and osmotic stressors such as drought and high salt. With reduced yields under drought conditions at the flowering stage, OsNAC2 overexpression lines had lower resistance to high salt and drought conditions. RNAi plants showed enhanced tolerance to high salinity and drought stress at both the vegetative and flowering stages. Furthermore, RNAi plants had improved yields after drought stress...
January 11, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28074634/arf2-coordinates-with-plethoras-and-pins-to-orchestrate-aba-mediated-root-meristem-activity-in-arabidopsis%C3%A2
#16
Sujittra Promchuea, Yujuan Zhu, Zhizhong Chen, Jing Zhang, Zhizhong Gong
Multiple hormones, including abscisic acid (ABA) and auxin, regulate cell division and differentiation of Arabidopsis root meristems. AUXIN RESPONSE FACTOR 2 (ARF2) functions as a negative regulator of ABA responses, as seed germination and primary root growth of arf2 mutants are hypersensitive to ABA. In this study, we found that ABA treatment reduced the expression levels of the PIN-FORMEDs (PIN) auxin efflux carriers, PIN1, PIN3, PIN4, and PIN7, to a greater extent in the root meristems of arf2-101 mutant than in the wild type...
January 2017: Journal of Integrative Plant Biology
https://www.readbyqxmd.com/read/28073772/a-water-availability-gradient-reveals-the-deficit-level-required-to-affect-traits-in-potted-juvenile-eucalyptus-globulus
#17
Adam B McKiernan, Brad M Potts, Mark J Hovenden, Timothy J Brodribb, Noel W Davies, Thomas Rodemann, Scott A M McAdam, Julianne M O'Reilly-Wapstra
BACKGROUND AND AIMS: Drought leading to soil water deficit can have severe impacts on plants. Water deficit may lead to plant water stress and affect growth and chemical traits. Plant secondary metabolite (PSM) responses to water deficit vary between compounds and studies, with inconsistent reports of changes to PSM concentrations even within a single species. This disparity may result from experimental water deficit variation among studies, and so multiple water deficit treatments are used to fully assess PSM responses in a single species...
January 10, 2017: Annals of Botany
https://www.readbyqxmd.com/read/28070585/effect-of-proline-on-biochemical-and-molecular-mechanisms-in-lettuce-lactuca-sativa-l-exposed-to-uv-b-radiation
#18
Ozkan Aksakal, Dilruba Tabay, Aslıhan Esringu, Feyza Icoglu Aksakal, Nevzat Esim
The purpose of the present study was to evaluate the role of proline (Pro) in relieving UV-B radiation-induced oxidative stress in lettuce. Lettuce seedlings were exposed to 3.3 W m(-2) UV-B radiation for 12 h after pre-treatment sprayed with 20 mM Pro. The data for malondialdehyde (MDA), hydrogen peroxide (H2O2), endogenous Pro level, the activities of antioxidant enzymes [superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX), and peroxidase (POD)], total phenolic concentration, antioxidant capacity, expression of phenylalanine ammonia lyase (PAL), γ-tocopherol methyltransferase (γ-TMT) and proline dehydrogenase (ProDH) genes, phytohormone levels such as abscisic acid (ABA), gibberellic acid (GA), indole acetic acid (IAA) and salicylic acid (SA), soluble sugars and organic acids were recorded...
January 10, 2017: Photochemical & Photobiological Sciences
https://www.readbyqxmd.com/read/28068641/oil-palm-drought-inducible-dreb1-induced-expression-of-dre-crt-and-non-dre-crt-containing-genes-in-lowland-transgenic-tomato-under-cold-and-peg-treatments
#19
Azzreena Mohamad Azzeme, Siti Nor Akmar Abdullah, Maheran Abd Aziz, Puteri Edaroyati Megat Wahab
Dehydration-responsive element binding (DREB) transcription factor plays an important role in controlling the expression of abiotic stress responsive genes. An intronless oil palm EgDREB1 was isolated and confirmed to be a nuclear localized protein. Electrophoretic mobility shift and yeast one-hybrid assays validated its ability to interact with DRE/CRT motif. Its close evolutionary relation to the dicot NtDREB2 suggests a universal regulatory role. In order to determine its involvement in abiotic stress response, functional characterization was performed in oil palm seedlings subjected to different levels of drought severity and in EgDREB1 transgenic tomato seedlings treated by abiotic stresses...
December 27, 2016: Plant Physiology and Biochemistry: PPB
https://www.readbyqxmd.com/read/28068551/investigating-task-inhibition-in-children-versus-adults-a-diffusion-model-analysis
#20
Stefanie Schuch, Kerstin Konrad
One can take n-2 task repetition costs as a measure of inhibition on the level of task sets. When switching back to a Task A after only one intermediate trial (ABA task sequence), Task A is thought to still be inhibited, leading to performance costs relative to task sequences where switching back to Task A is preceded by at least two intermediary trials (CBA). The current study investigated differences in inhibitory ability between children and adults by comparing n-2 task repetition costs in children (9-11years of age, N=32) and young adults (21-30years of age, N=32)...
January 6, 2017: Journal of Experimental Child Psychology
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