keyword
MENU ▼
Read by QxMD icon Read
search

Hmox1

keyword
https://www.readbyqxmd.com/read/29328656/design-and-synthesis-of-novel-reactive-oxygen-species-inducers-for-the-treatment-of-pancreatic-ductal-adenocarcinoma
#1
Yuting Kuang, Mario Sechi, Salvatore Nurra, Mats Ljungman, Nouri Neamati
Altering redox homeostasis provides distinctive therapeutic opportunities for the treatment of pancreatic cancer. Quinazolinediones (QDs) are novel redox modulators that we previously showed to induce potent growth inhibition in pancreatic ductal adenocarcinoma (PDAC) cell lines. Our lead optimization campaign yielded QD325 as the most potent redox modulator candidate inducing substantial reactive oxygen species (ROS) in PDAC cells. Nascent RNA sequencing following treatments with the QD compounds revealed induction of stress responses in nucleus, endoplasmic reticulum, and mitochondria of pancreatic cancer cells...
January 12, 2018: Journal of Medicinal Chemistry
https://www.readbyqxmd.com/read/29316264/heme-oxygenase-1-affects-generation-and-spontaneous-cardiac-differentiation-of-induced-pluripotent-stem-cells
#2
Jacek Stepniewski, Tomasz Pacholczak, Aniela Skrzypczyk, Maciej Ciesla, Agata Szade, Krzysztof Szade, Romain Bidanel, Agnieszka Langrzyk, Radoslaw Grochowski, Felix Vandermeeren, Neli Kachamakova-Trojanowska, Mateusz Jez, Grazyna Drabik, Mahito Nakanishi, Alicja Jozkowicz, Jozef Dulak
Cellular stress can influence efficiency of iPSCs generation and their differentiation. However, the role of intracellular cytoprotective factors in these processes is still not well known. Therefore, we investigated the effect of HO-1 (Hmox1) or Nrf2 (Nfe2l2), two major cytoprotective genes. Hmox1-/- fibroblasts demonstrated decreased reprogramming efficiency in comparison to Hmox1+/+ cells. Reversely, pharmacological enhancement of HO-1 resulted in higher number of iPSCs colonies. Importantly, elevated level of both p53 and p53-regulated miR-34a and 14-3-3σ was observed in HO-1-deficient fibroblasts whereas downregulation of p53 in these cells markedly increased their reprogramming efficiency...
January 9, 2018: IUBMB Life
https://www.readbyqxmd.com/read/29291526/assessment-of-lung-cell-toxicity-of-various-gasoline-engine-exhausts-using-a-versatile-in%C3%A2-vitro-exposure-system
#3
Christoph Bisig, Pierre Comte, Martin Güdel, Jan Czerwinski, Andreas Mayer, Loretta Müller, Alke Petri-Fink, Barbara Rothen-Rutishauser
Adverse effect studies of gasoline exhaust are scarce, even though gasoline direct injection (GDI) vehicles can emit a high number of particles. The aim of this study was to conduct an in vitro hazard assessment of different GDI exhausts using two different cell culture models mimicking the human airway. In addition to gasoline particle filters (GPF), the effects of two lubrication oils with low and high ash content were assessed, since it is known that oils are important contributors to exhaust emissions...
December 29, 2017: Environmental Pollution
https://www.readbyqxmd.com/read/29259394/characterizing-the-effect-of-supplements-on-the-phenotype-of-cultured-macrophages-from-patients-with-age-related-macular-degeneration
#4
Batya Rinsky, Shira Hagbi-Levi, Sarah Elbaz-Hayoun, Michelle Grunin, Itay Chowers
Purpose: Oral vitamin and mineral supplements reduce the risk of visual loss in age-related macular degeneration (AMD). However, the pathways that mediate this beneficial effect are poorly understood. Macrophages may exert oxidative, inflammatory, and angiogenic effects in the context of AMD. We aim to assess if oral supplements can modulate the macrophage phenotype in this disease. Methods: Monocytes were isolated from patients with neovascular AMD (nvAMD), cultured, matured to macrophages, and polarized to classical [M1 (stimulated by IFNγ and lipopolysaccharide (LPS))] and alternative [M2 (stimulated with IL-4 and IL-13)] phenotypes...
2017: Molecular Vision
https://www.readbyqxmd.com/read/29247444/effect-of-arsenic-exposure-on-nrf2-keap1-pathway-and-epigenetic-modification
#5
Beata Janasik, Edyta Reszka, Magdalena Stanislawska, Ewa Jablonska, Renata Kuras, Edyta Wieczorek, Beata Malachowska, Wojciech Fendler, Wojciech Wasowicz
Arsenic (As) is a known toxic element and carcinogen. Transcription factor nuclear factor-erythroid 2-related factor 2 (NRF2) controls cellular adaptation to oxidants and electrophiles by inducing antioxidant genes in response to redox stress. To explore associations between As level and NRF2-regulated cytoprotective genes expression, an observational study was conducted in a population of 61 occupationally exposed men with median (Me) age 50 years (interquartile range (IQR) 42-54) and in a control group of 52 men aged 40 (IQR 31-51...
December 15, 2017: Biological Trace Element Research
https://www.readbyqxmd.com/read/29203625/fetal-microsatellite-in-the-heme-oxygenase-1-promoter-is-associated-with-severe-and-early-onset-preeclampsia
#6
Tea Kaartokallio, Siddheshwar Utge, Miira M Klemetti, Jussi Paananen, Kari Pulkki, Jarkko Romppanen, Ilkka Tikkanen, Seppo Heinonen, Eero Kajantie, Juha Kere, Katja Kivinen, Anneli Pouta, Päivi Lakkisto, Hannele Laivuori
Preeclampsia is a vascular pregnancy disorder that often involves impaired placental development. HO-1 (heme oxygenase 1, encoded by HMOX1) is a stress response enzyme crucial for endothelial and placental function. Long version of the guanine-thymine (GTn) microsatellite in the HMOX1 promoter decreases HO-1 expression, and the long maternal repeat is associated with late-onset preeclampsia. Our aim was to study whether the length of fetal repeat is associated with mother's preeclampsia, whether the length of fetal and maternal repeats affect HO-1 levels in placenta and maternal serum, and whether HO-1 levels are altered in preeclampsia...
December 4, 2017: Hypertension
https://www.readbyqxmd.com/read/29194609/using-the-connectivity-map-to-discover-compounds-influencing-human-osteoblast-differentiation
#7
A M Brum, J van de Peppel, L Nguyen, A Aliev, M Schreuders-Koedam, T V Gajadien, C S van der Leije, A van Kerkwijk, M Eijken, J P T M van Leeuwen, B C J van der Eerden
Osteoporosis is a common skeletal disorder characterized by low bone mass leading to increased bone fragility and fracture susceptibility. Identification of factors influencing osteoblast differentiation and bone formation is very important. Previously, we identified parbendazole to be a novel compound that stimulates osteogenic differentiation of human mesenchymal stromal cells (hMSCs), using gene expression profiling and bioinformatic analyses, including the Connectivity Map (CMap), as an in-silico approach...
December 1, 2017: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/29169990/lack-of-heme-oxygenase-1-induces-inflammatory-reaction-and-proliferation-of-muscle-satellite-cells-after-cardiotoxin-induced-skeletal-muscle-injury
#8
Magdalena Kozakowska, Katarzyna Pietraszek-Gremplewicz, Maciej Ciesla, Marta Seczynska, Iwona Bronisz-Budzynska, Paulina Podkalicka, Karolina Bukowska-Strakova, Agnieszka Loboda, Alicja Jozkowicz, Jozef Dulak
Heme oxygenase-1 (HO-1, Hmox1) regulates viability, proliferation, and differentiation of many cell types, hence it may affect regeneration of injured skeletal muscle. Here we injected cardiotoxin into gastrocnemius muscle of Hmox1+/+ and Hmox1-/- animals and analyzed cellular response after muscle injury, focusing on muscle satellite cells (SC), inflammatory reaction, fibrosis, and formation of new blood vessels. HO-1 is strongly induced after muscle injury, being expressed mostly in the infiltrating leukocytes (CD45+ cells), including macrophages (F4/80+ cells)...
November 20, 2017: American Journal of Pathology
https://www.readbyqxmd.com/read/29167567/real-time-in-vivo-imaging-reveals-localised-nrf2-stress-responses-associated-with-direct-and-metabolism-dependent-drug-toxicity
#9
Shiva S Forootan, Fiona E Mutter, Anja Kipar, Takao Iwawaki, Ben Francis, Christopher E Goldring, B Kevin Park, Ian M Copple
The transcription factor Nrf2 coordinates an adaptive response to chemical and oxidative stress characterised by the upregulated expression of cytoprotective target genes. In order to understand the mechanistic relevance of Nrf2 as a marker of drug-induced stress it is important to know if this adaptive response is truly localised in the context of organ-specific drug toxicity. Here, we address this knowledge gap through real-time bioluminescence imaging of transgenic Nrf2-luciferase (Nrf2-luc) reporter mice following administration of the metabolism-dependent hepatotoxin acetaminophen (APAP) or the direct nephrotoxin cisplatin...
November 22, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29161307/repeat-polymorphisms-in-the-homo-sapiens-heme-oxygenase-1-gene-in-diabetic-and-idiopathic-gastroparesis
#10
Simon J Gibbons, Madhusudan Grover, Kyoung Moo Choi, Akhilesh Wadhwa, Adeel Zubair, Laura A Wilson, Yanhong Wu, Thomas L Abell, William L Hasler, Kenneth L Koch, Richard W McCallum, Linda A B Nguyen, Henry P Parkman, Irene Sarosiek, William J Snape, James Tonascia, Frank A Hamilton, Pankaj J Pasricha, Gianrico Farrugia
BACKGROUND: Idiopathic and diabetic gastroparesis in Homo sapiens cause significant morbidity. Etiology or risk factors have not been clearly identified. Failure to sustain elevated heme oxygenase-1 (HO1) expression is associated with delayed gastric emptying in diabetic mice and polymorphisms in the HO1 gene (HMOX1, NCBI Gene ID:3162) are associated with worse outcomes in other diseases. AIM: Our hypothesis was that longer polyGT alleles are more common in the HMOX1 genes of individuals with gastroparesis than in controls without upper gastrointestinal motility disorders...
2017: PloS One
https://www.readbyqxmd.com/read/29155359/4-methylthio-3-butenyl-isothiocyanate-mediates-nuclear-factor-erythroid-derived-2-like-2-activation-by-regulating-reactive-oxygen-species-production-in-human-esophageal-epithelial-cells
#11
Tadashi Hirata, Young-Man Cho, Isamu Suzuki, Takeshi Toyoda, Jun-Ichi Akagi, Yasushi Nakamura, Satoshi Numazawa, Kumiko Ogawa
4-Methylthio-3-butenyl isothiocyanate (MTBITC) extracted from daikon (Raphanus sativus), which shows antimutagenicity, may have applications as an effective chemopreventive agent in several cancers; however, few reports have described the associated mechanisms. We investigated whether MTBITC induced cytoprotective genes, including phase II enzymes, in Het-1A human esophageal epithelial cells. HMOX1, NQO1, and GCLC mRNA levels and nuclear factor (erythroid-derived 2)-like 2 (Nrf2) protein levels were increased in Het-1A cells exposed to 10 μM MTBITC...
November 16, 2017: Food and Chemical Toxicology
https://www.readbyqxmd.com/read/29142166/fish-oil-feeding-reverses-hepatomegaly-and-disrupted-hepatic-function-due-to-the-lack-of-fxr-signaling
#12
Masaaki Miyata, Kouhei Shinno, Tomoki Kinoshita, Yuichi Kinoshita, Yoshimasa Sugiura
Mice lacking farnesoid X receptor (FXR) are used as a model for nonalcoholic fatty liver disease because their livers exhibit hepatomegaly, hepatic steatosis, and hepatic inflammation. The influence of fish oil feeding on hepatomegaly and disrupted hepatic function was investigated using female Fxr-null mice and wild-type mice fed a fish oil diet (2% fish oil and 2% corn oil) or a control diet (4% corn oil) for 4 weeks. Hepatic n-3 polyunsaturated fatty acid (PUFA) levels, including 22:6 n-3 docosahexaenoic acid (DHA) and 20:5 n-3 eicosapentaenoic acid (EPA) were significantly higher in the fish oil group than those in the control group of Fxr-null mice and wild-type mice...
2017: Journal of Toxicological Sciences
https://www.readbyqxmd.com/read/29096690/tlr2-stimulation-impairs-anti-inflammatory-activity-of-m2-like-macrophages-generating-a-chimeric-m1-m2-phenotype
#13
Lilian Quero, Edveena Hanser, Tobias Manigold, André N Tiaden, Diego Kyburz
BACKGROUND: Toll-like receptors (TLRs) and macrophages play an important role in rheumatoid arthritis (RA). Currently, it is not clear whether inflammatory M1 or anti-inflammatory M2 predominate among the resident macrophages in the synovium. In the present study, we set out to investigate the impact of TLR stimulation on monocyte-derived M1 and M2 macrophage function and phenotype by mimicking the exposure to abundant TLR agonists as occurs in the context of RA. The response of macrophage subsets to TLR2 and TLR4 activation was evaluated on cluster of differentiation (CD) marker profile; cytokine secretion; gene expression; and NF-κB, interferon regulatory factors 3 and 7 (IRF3/7), and mitogen-activated protein kinase (MAPK) activation...
November 2, 2017: Arthritis Research & Therapy
https://www.readbyqxmd.com/read/29082606/increased-small-intestine-expression-of-non-heme-iron-transporters-in-morbidly-obese-patients-with-newly-diagnosed-type-2-diabetes
#14
José María Moreno-Navarrete, Amaia Rodríguez, Sara Becerril, Víctor Valentí, Javier Salvador, Gema Frühbeck, José Manuel Fernández-Real
SCOPE: To investigate intestinal markers of iron absorption in morbidly obese subjects according to glucose tolerance. METHODS AND RESULTS: Gene expression of both non-heme [SLC40A1 (ferroportin), SLC11A2] and heme iron [SLC46A1 (HCP1), HMOX1] transporters were analysed in 38 small intestine tissue samples [11 with normal glucose tolerance, 14 with glucose intolerance (GI) and 13 with newly diagnosed type 2 diabetes (T2D)]. SLC40A1 (r = 0.43, p = 0.008) and SLC11A2 (r = 0...
October 29, 2017: Molecular Nutrition & Food Research
https://www.readbyqxmd.com/read/29074538/tgf-%C3%AE-promotes-genomic-instability-after-loss-of-runx3
#15
Vaidehi Krishnan, Yu Lin Chong, Tuan Zea Tan, Madhura D Kulkarni, Muhammad Bakhait Bin Rahmat, Lavina Sierra Tay, Doorgesh S Jokhun, Amudha Ganesan, Linda Shyue Huey Chuang, Dominic Chih-Cheng Voon, Shivashankar Gv, Jean Paul Thiery, Yoshiaki Ito
Studies of genomic instability have historically focused on intrinsic mechanisms rather than extrinsic mechanisms based on the tumor microenvironment (TME). TGF-β is the most abundantly secreted cytokine in the TME where it imparts various aggressive characteristics including invasive migration, drug resistance and epithelial-to-mesenchymal transition (EMT). Here we show that TGF-β also promotes genomic instability in the form of DNA double strand breaks (DSB) in cancer cells which lack the tumor suppressor gene RUNX3...
October 26, 2017: Cancer Research
https://www.readbyqxmd.com/read/29065700/murine-bone-marrow-mesenchymal-stromal-cells-respond-efficiently-to-oxidative-stress-despite-the-low-level-of-heme-oxygenases-1-and-2
#16
Witold Norbert Nowak, Hevidar Taha, Neli Kachamakova-Trojanowska, Jacek Stepniewski, Joanna Agata Markiewicz, Anna Kusienicka, Krzysztof Szade, Agata Szade, Karolina Bukowska-Strakova, Karolina Hajduk, Damian Klóska, Aleksandra Kopacz, Anna Grochot-Przeczek, Kathrin Barthenheier, Camille Cauvin, Jozef Dulak, Alicja Jozkowicz
AIMS: Mesenchymal stromal cells (MSCs) are heterogeneous cells from adult tissues, able to differentiate in vitro into adipocytes, osteoblasts or chondrocytes. Such cells are widely studied in regenerative medicine. However, the success of cellular therapy depends on the cell survival. Heme oxygenase-1 (HO-1, encoded by the Hmox1 gene), an enzyme converting heme to biliverdin, carbon monoxide, and Fe2+, is cytoprotective and can affect stem cell performance. Therefore, our study aimed to assess if Hmox1 is critical for survival and functions of murine bone marrow MSCs...
October 25, 2017: Antioxidants & Redox Signaling
https://www.readbyqxmd.com/read/29056901/interleukin-10-attenuates-hypochlorous-acid-mediated-cytotoxicity-to-hei-oc1-cochlear-cells
#17
Martin Mwangi, Sung-Hee Kil, David Phak, Hun Yi Park, David J Lim, Raekil Park, Sung K Moon
Inflammatory reaction plays a crucial role in the pathophysiology of acquired hearing loss such as ototoxicity and labyrinthitis. In our earlier work, we showed the pivotal role of otic fibrocytes in cochlear inflammation and the critical involvement of proinflammatory cytokines in cisplatin ototoxicity. We also demonstrated that otic fibrocytes inhibit monocyte chemoattractant protein 1 (CCL2) upregulation in response to interleukin-10 (IL-10) via heme oxygenase 1 (HMOX1) signaling, resulting in suppression of cochlear inflammation...
2017: Frontiers in Cellular Neuroscience
https://www.readbyqxmd.com/read/29050310/microrna-27b-inhibition-promotes-nrf2-are-pathway-activation-and-alleviates-intracerebral-hemorrhage-induced-brain-injury
#18
Wenzhe Xu, Feng Li, Zhiguo Liu, Zhenkuan Xu, Bin Sun, Jingwei Cao, Yuguang Liu
Oxidative stress and neuroinflammation are the key factors leading to secondary brain injury after intracerebral hemorrhage (ICH). We investigated the effects of miR-27b, an oxidative stress-responsive microRNA, on ICH-induced brain injury in rats. The ICH model was induced by intracerebral injection of collagenase. Following ICH, miR-27b expression in the striatum was reduced, whereas expression of Nrf2 mRNA and protein was increased. In PC12 cells, overexpression of miR-27b reduced expression of Nrf2, Hmox1, Sod1 and Nqo1, while miR-27b inhibition had the opposite effects...
September 19, 2017: Oncotarget
https://www.readbyqxmd.com/read/29048948/the-spectrum-of-sickle-hemoglobin-related-nephropathy-from-sickle-cell-disease-to-sickle-trait
#19
Rakhi P Naik, Vimal K Derebail
Renal dysfunction is among the most common complication of sickle cell disease (SCD), from hyposthenuria in children to progression to overt chronic kidney disease (CKD) in young adults. Emerging evidence now suggests that sickle hemoglobin-related nephropathy extends to individuals with sickle cell trait (SCT). Areas covered: This review will highlight the pathophysiology, epidemiology, and management recommendations for sickle hemoglobin-related nephropathy in both SCD and SCT. In addition, it will focus on the major demographic and genetic modifiers of renal disease in sickling hemoglobinopathies...
December 2017: Expert Review of Hematology
https://www.readbyqxmd.com/read/29033950/glutathione-fine-tunes-the-innate-immune-response-toward-antiviral-pathways-in-a-macrophage-cell-line-independently-of-its-antioxidant-properties
#20
Marina Diotallevi, Paola Checconi, Anna Teresa Palamara, Ignacio Celestino, Lucia Coppo, Arne Holmgren, Kahina Abbas, Fabienne Peyrot, Manuela Mengozzi, Pietro Ghezzi
Glutathione (GSH), a major cellular antioxidant, is considered an inhibitor of the inflammatory response involving reactive oxygen species (ROS). However, evidence is largely based on experiments with exogenously added antioxidants/reducing agents or pro-oxidants. We show that depleting macrophages of 99% of GSH does not exacerbate the inflammatory gene expression profile in the RAW264 macrophage cell line or increase expression of inflammatory cytokines in response to the toll-like receptor 4 (TLR4) agonist lipopolysaccharide (LPS); only two small patterns of LPS-induced genes were sensitive to GSH depletion...
2017: Frontiers in Immunology
keyword
keyword
73593
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"