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https://www.readbyqxmd.com/read/28432984/cigarette-smoke-extract-counteracts-atheroprotective-effects-of-high-laminar-flow-on-endothelial-function
#1
Sindy Giebe, Natalia Cockcroft, Katherine Hewitt, Melanie Brux, Anja Hofmann, Henning Morawietz, Coy Brunssen
Tobacco smoking and hemodynamic forces are key stimuli in the development of endothelial dysfunction and atherosclerosis. High laminar flow has an atheroprotective effect on the endothelium and leads to a reduced response of endothelial cells to cardiovascular risk factors compared to regions with disturbed or low laminar flow. We hypothesize that the atheroprotective effect of high laminar flow could delay the development of endothelial dysfunction caused by cigarette smoking. Primary human endothelial cells were stimulated with increasing dosages of aqueous cigarette smoke extract (CSEaq)...
April 7, 2017: Redox Biology
https://www.readbyqxmd.com/read/28431930/quantitative-proteomics-reveals-key-proteins-regulated-by-eicosapentaenoic-acid-in-endothelial-activation
#2
Lu Zhang, Kunhong Xiao, Xuyang Zhao, Xiaoli Sun, Jianshu Zhang, Xian Wang, Yi Zhu, Xu Zhang
Eicosapentaenoic acid (EPA), an n-3 polyunsaturated fatty acid (PUFA), has been shown to decrease the risk of atherosclerosis by attenuating endothelial activation. In this study, we used mass spectrometry-based label-free quantitative proteomics to study the protective mechanisms of EPA and to identify key proteins that regulated by EPA in endothelial activation. Arachidonic acid (AA) was used as a control. HUVECs were pretreated with each of the two PUFAs, and then stimulated with TNFα as a model of endothelial activation...
April 18, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28422321/aberrantly-expressed-long-non-coding-rnas-in-cd8-t-cells-response-to-active-tuberculosis
#3
Yurong Fu, Kunshan Gao, Enxue Tao, Ruifang Li, Zhengjun Yi
Dysregulated expression of long noncoding RNAs (lncRNAs) has been demonstrated as being implicated in a variety of human diseases. In the study we aimed to determine lncRNA profile in CD8(+) T cells response to active tuberculosis (TB). We examined the lncRNA expression by microarray in circulating CD8(+) T cells isolated from patients with active TB and healthy controls. Change predictions to analysis was used to address functional roles of the deregulated mRNAs. Real-time quantitative PCR (RT-qPCR) was used to validate the microarray result...
April 19, 2017: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/28370325/mutagenicity-and-oxidative-damage-induced-by-an-organic-extract-of-the-particulate-emissions-from-a-simulation-of-the-deepwater-horizon-surface-oil-burns
#4
David M DeMarini, Sarah H Warren, Katelyn Lavrich, Alexis Flen, Johanna Aurell, William Mitchell, Dale Greenwell, William Preston, Judith E Schmid, William P Linak, Michael D Hays, James M Samet, Brian K Gullett
Emissions from oil fires associated with the "Deepwater Horizon" explosion and oil discharge that began on April 20, 2010 in the Gulf of Mexico were analyzed chemically to only a limited extent at the time but were shown to induce oxidative damage in vitro and in mice. To extend this work, we burned oil floating on sea water and performed extensive chemical analyses of the emissions (Gullett et al., Marine Pollut Bull, in press, ). Here, we examine the ability of a dichloromethane extract of the particulate material with an aerodynamic size ≤ 2...
April 2017: Environmental and Molecular Mutagenesis
https://www.readbyqxmd.com/read/28366867/cadmium-and-%C3%AE-lipoic-acid-activate-similar-de-novo-synthesis-and-recycling-pathways-for-glutathione-balance
#5
Jose Macias-Barragan, Selene G Huerta-Olvera, Ivan Hernandez-Cañaveral, Ana Laura Pereira-Suarez, Margarita Montoya-Buelna
Glutathione (GSH) protects cells against oxidative stress. Redox modifiers induce GSH biosynthesis and recycling to maintain reduced environment inside cells. Cadmium (Cd(2+)) is a heavy metal that activates redox-sensitive transcriptional factors. The antioxidant α-lipoic acid (ALA) has shown to modulate GSH pathways. This study aimed to investigate de novo synthesis and recycling pathways for GSH balance by different Cd(2+) concentrations and ALA in HepG2 cells. ALA activates Nrf2 pathway leading to GSH increment...
March 9, 2017: Environmental Toxicology and Pharmacology
https://www.readbyqxmd.com/read/28365246/the-association-between-oxidative-stress-induced-galectins-and-differentiation-of-human-promyelocytic-hl-60-cells
#6
James R Vinnai, Robert C Cumming, Graham J Thompson, Alexander V Timoshenko
Galectins are multifunctional β-galactoside-binding proteins that are involved in the regulation of cellular stress responses and differentiation. The relationship between these processes is unclear and we report here that galectins display oxidative-stress specific expression patterns in neutrophil-like differentiated HL-60 cells. Three galectins (-1, -3, and -10) are upregulated in response to either menadione or DMSO exposure whereas galectins -9 and -12 exhibited a stimulus-dependent downregulation. Changes in galectin expression are oxidant dependent based on the observations that 1) oxidative stress biomarkers HMOX1 (heme oxygenase-1) and NCF1 (neutrophil cytosolic factor 1, which is also a biomarker of neutrophil differentiation) are elevated in both cases, and 2) the antioxidant N-acetyl-L-cysteine restores basal expression of galectin-3 following oxidant exposure...
March 30, 2017: Experimental Cell Research
https://www.readbyqxmd.com/read/28365151/a-cell-surface-membrane-protein-signature-for-glioblastoma
#7
Dhimankrishna Ghosh, Cory C Funk, Juan Caballero, Nameeta Shah, Katherine Rouleau, John C Earls, Liliana Soroceanu, Greg Foltz, Charles S Cobbs, Nathan D Price, Leroy Hood
We present a systems strategy that facilitated the development of a molecular signature for glioblastoma (GBM), composed of 33 cell-surface transmembrane proteins. This molecular signature, GBMSig, was developed through the integration of cell-surface proteomics and transcriptomics from patient tumors in the REMBRANDT (n = 228) and TCGA datasets (n = 547) and can separate GBM patients from control individuals with a Matthew's correlation coefficient value of 0.87 in a lock-down test. Functionally, 17/33 GBMSig proteins are associated with transforming growth factor β signaling pathways, including CD47, SLC16A1, HMOX1, and MRC2...
March 28, 2017: Cell Systems
https://www.readbyqxmd.com/read/28363268/microrna-183-5p-increases-with-age-in-bone-derived-extracellular-vesicles-suppresses-bone-marrow-stromal-stem-cell-proliferation-and-induces-stem-cell-senescence
#8
Colleen Davis, Amy Dukes, Michelle Drewry, Inas Helwa, Maribeth Johnson, Carlos M Isales, William D Hill, Yutao Liu, Xingming Shi, Sadanand Fulzele, Mark W Hamrick
Microvesicle- and exosome-mediated transport of microRNAs (miRNAs) represents a novel cellular and molecular pathway for cell-cell communication. Here we tested the hypothesis that these extracellular vesicles (EVs) and their miRNAs might change with age, contributing to age-related stem cell dysfunction. EVs were isolated from the bone marrow interstitial fluid (supernatant) of young (3-4 mo) and aged (24-28 mo) mice to determine whether the size, concentration, and miRNA profile of EVs was altered with age in vivo...
March 31, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/28334711/quantitative-proteomics-analysis-of-ischemia-reperfusion-injury-modulated-proteins-in-cardiac-microvascular-endothelial-cells-and-the-protective-role-of-tongxinluo
#9
Qing Li, He-He Cui, Yue-Jin Yang, Xiang-Dong Li, Gui-Hao Chen, Xia-Qiu Tian, Chen Jin, Qiu-Ting Dong, Pei-Sen Huang, Jun Xu
BACKGROUND: The protection of endothelial cells (ECs) against reperfusion injury has received little attention. In this study, we used Tandem Mass Tag (TMT) labeling proteomics to investigate the modulated proteins in an in vitro model of cardiac microvascular endothelial cells (CMECs) subjected to ischemia/reperfusion (I/R) injury and their alteration by traditional Chinese medicine Tongxinluo (TXL). METHODS: Human CMECs were subjected to 2 h of hypoxia followed by 2 h of reoxygenation with different concentrations of TXL Protein expression profiles of CMECs were determined using tandem mass spectrometry...
March 24, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/28323022/astrocyte-heme-oxygenase-1-reduces-mortality-and-improves-outcome-after-collagenase-induced-intracerebral-hemorrhage
#10
Jing Chen-Roetling, Pramod Kamalapathy, Yang Cao, Wei Song, Hyman M Schipper, Raymond F Regan
Pharmacotherapies that increase CNS expression of heme oxygenase-1 (HO-1) and other antioxidant proteins have improved outcome in experimental models of spontaneous intracerebral hemorrhage (ICH). In order to more specifically investigate the relationship between HO-1 and ICH outcome, mice expressing human HO-1 driven by the glial fibrillary acidic protein (GFAP) promoter (GFAP·HMOX1 mice) were tested in a model of in situ parenchymal hemorrhage. Injection of collagenase into the striata of wild-type (WT) mice resulted in a 26...
June 2017: Neurobiology of Disease
https://www.readbyqxmd.com/read/28300285/hx-1171-a-novel-nrf2-activator-induces-nqo1-and-hmox1-expression
#11
Jimin Kim, Su-Hyun Shin, Young-Eun Ko, Tokutaro Miki, Heung-Mo Bae, Jong-Koo Kang, Jae Wha Kim
HX-1171 (1-O-hexyl-2,3,5-trimethylhydroquinone) is a novel synthesized vitamin E derivative, which reportedly has positive effects on various diseases and conditions, such as liver fibrosis, hepatic cirrhosis, and cancer. In this study, we analyzed the transcriptional activity induced by HX-1171. Results from reverse transcription polymerase chain reaction and promoter assays reveal that HX-1171 increased the expression of NQO1 and HMOX1, encoding antioxidant-related enzymes, in A549 human lung epithelial cells...
March 16, 2017: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/28289476/dna-methylation-at-modifier-genes-of-lung-disease-severity-is-altered-in-cystic-fibrosis
#12
Milena Magalhães, Isabelle Rivals, Mireille Claustres, Jessica Varilh, Mélodie Thomasset, Anne Bergougnoux, Laurent Mely, Sylvie Leroy, Harriet Corvol, Loïc Guillot, Marlène Murris, Emmanuelle Beyne, Davide Caimmi, Isabelle Vachier, Raphaël Chiron, Albertina De Sario
BACKGROUND: Lung disease progression is variable among cystic fibrosis (CF) patients and depends on DNA mutations in the CFTR gene, polymorphic variations in disease modifier genes, and environmental exposure. The contribution of genetic factors has been extensively investigated, whereas the mechanism whereby environmental factors modulate the lung disease is unknown. In this project, we hypothesized that (i) reiterative stress alters the epigenome in CF-affected tissues and (ii) DNA methylation variations at disease modifier genes modulate the lung function in CF patients...
2017: Clinical Epigenetics
https://www.readbyqxmd.com/read/28276576/delta-amino-levulinic-acid-dehydratase-gene-and-essential-tremor
#13
José A G Agúndez, Elena García-Martín, Hortensia Alonso-Navarro, Pedro Ayuso, Gara Esguevillas, Julián Benito-León, Sara Ortega-Cubero, Pau Pastor, Tomás López-Alburquerque, Félix Javier Jiménez-Jiménez
BACKGROUND/OBJECTIVE: Several reports found a relationship between increased serum lead levels and the risk for essential tremor (ET), especially in carriers of the minor allele of the single nucleotide polymorphism (SNP) rs1800435 in the aminolevulinate dehydratase (ALAD) gene, which is involved in the synthesis of heme groups. Our group reported decreased risk for ET in carriers of the minor alleles of the rs2071746 and rs1051308 SNPs in the heme-oxygenases 1 and 2 (HMOX1 and HMOX2), respectively, involved in heme metabolism...
March 9, 2017: European Journal of Clinical Investigation
https://www.readbyqxmd.com/read/28270832/actin-family-proteins-in-the-human-ino80-chromatin-remodeling-complex-exhibit-functional-roles-in-the-induction-of-heme-oxygenase-1-with-hemin
#14
Yuichiro Takahashi, Hirokazu Murakami, Yusuke Akiyama, Yasutake Katoh, Yukako Oma, Hitoshi Nishijima, Kei-Ichi Shibahara, Kazuhiko Igarashi, Masahiko Harata
Nuclear actin family proteins, comprising of actin and actin-related proteins (Arps), are essential functional components of the multiple chromatin remodeling complexes. The INO80 chromatin remodeling complex, which is evolutionarily conserved and has roles in transcription, DNA replication and repair, consists of actin and actin-related proteins Arp4, Arp5, and Arp8. We generated Arp5 knockout (KO) and Arp8 KO cells from the human Nalm-6 pre-B cell line and used these KO cells to examine the roles of Arp5 and Arp8 in the transcriptional regulation mediated by the INO80 complex...
2017: Frontiers in Genetics
https://www.readbyqxmd.com/read/28264691/endothelial-responses-of-the-alveolar-barrier-in-vitro-in-a-dose-controlled-exposure-to-diesel-exhaust-particulate-matter
#15
Sebastian G Klein, Sébastien Cambier, Jennifer Hennen, Sylvain Legay, Tommaso Serchi, Inge Nelissen, Aline Chary, Elisa Moschini, Andreas Krein, Brunhilde Blömeke, Arno C Gutleb
BACKGROUND: During the last 250 years, the level of exposure to combustion-derived particles raised dramatically in western countries, leading to increased particle loads in the ambient air. Among the environmental particles, diesel exhaust particulate matter (DEPM) plays a special role because of its omnipresence and reported effects on human health. During recent years, a possible link between air pollution and the progression of atherosclerosis is recognized. A central effect of DEPM is their impact on the endothelium, especially of the alveolar barrier...
March 6, 2017: Particle and Fibre Toxicology
https://www.readbyqxmd.com/read/28257713/hmox1-promoter-microsatellite-polymorphism-is-not-associated-with-high-altitude-pulmonary-edema-in-han-chinese
#16
Xue-Feng Cao, Lan Ma, Shang Ma, Jin Xu, Ri-Li Ge
OBJECTIVE: To investigate the relationship between microsatellite polymorphism in the Heme oxygenase-1 (HMOX1) gene promoter and high-altitude pulmonary edema (HAPE) in Han Chinese. METHODS: Eighty-three construction workers who developed HAPE 2 to 7 days after arrival at Yushu (3800 m) in Qinghai, China, and 145 matched healthy subjects were included in this study. The amplification and labeling of the polymerase chain reaction products for capillary electrophoresis were performed to identify HMOX1 genotype frequency...
March 2017: Wilderness & Environmental Medicine
https://www.readbyqxmd.com/read/28250190/activation-of-nrf2-antioxidant-signaling-by-1-25-dihydroxycholecalciferol-prevents-leptin-induced-oxidative-stress-and-inflammation-in-human-endothelial-cells
#17
Thaisa M Teixeira, Danielly C da Costa, Angela C Resende, Christophe O Soulage, Flavia F Bezerra, Julio B Daleprane
Background: Obesity is associated with hyperleptinemia and endothelial dysfunction. Hyperleptinemia has been reported to induce both oxidative stress and inflammation by increasing reactive oxygen species production.Objective: The objective of this study was to determine the effects of 1,25-dihydroxycholecalciferol [1,25(OH)2D3] against leptin-induced oxidative stress and inflammation in human endothelial cells.Methods: Small interfering RNA (siRNA) were used to knock down the expression of vitamin D receptor (VDR) in human umbilical vein endothelial cells (HUVECs)...
March 1, 2017: Journal of Nutrition
https://www.readbyqxmd.com/read/28243792/hmox1-as-a-marker-of-iron-excess-induced-adipose-tissue-dysfunction-affecting-glucose-uptake-and-respiratory-capacity-in-human-adipocytes
#18
José María Moreno-Navarrete, Francisco Ortega, Amaia Rodríguez, Jèssica Latorre, Sara Becerril, Mònica Sabater-Masdeu, Wifredo Ricart, Gema Frühbeck, José Manuel Fernández-Real
AIMS/HYPOTHESIS: Iron excess in adipose tissue is known to promote adipose tissue dysfunction. Here, we aimed to investigate the possible role of haem oxygenase 1 (HMOX1) in iron excess-induced adipose tissue dysfunction. METHODS: Cross-sectionally, HMOX1 gene expression in subcutaneous and visceral adipose tissue was analysed in two independent cohorts (n = 234 and 40) in relation to obesity. We also evaluated the impact of weight loss (n = 21), weight gain (in rats, n = 20) on HMOX1 mRNA; HMOX1 mRNA levels during human adipocyte differentiation; the effects of inflammation and iron on adipocyte HMOX1; and the effects of HMOX1-induced activity on adipocyte mitochondrial respiratory function, glucose uptake and adipogenesis...
February 27, 2017: Diabetologia
https://www.readbyqxmd.com/read/28238834/identification-of-potential-target-genes-of-ror-alpha-in-thp1-and-huvec-cell-lines
#19
Cagri Gulec, Neslihan Coban, Bilge Ozsait-Selcuk, Sema Sirma-Ekmekci, Ozlem Yildirim, Nihan Erginel-Unaltuna
ROR-alpha is a nuclear receptor, activity of which can be modulated by natural or synthetic ligands. Due to its possible involvement in, and potential therapeutic target for atherosclerosis, we aimed to identify ROR-alpha target genes in monocytic and endothelial cell lines. We performed chromatin immunoprecipitation (ChIP) followed by tiling array (ChIP-on-chip) for ROR-alpha in monocytic cell line THP1 and endothelial cell line HUVEC. Following bioinformatic analysis of the array data, we tested four candidate genes in terms of dependence of their expression level on ligand-mediated ROR-alpha activity, and two of them in terms of promoter occupancy by ROR-alpha...
February 24, 2017: Experimental Cell Research
https://www.readbyqxmd.com/read/28229933/nuclear-lamins-and-progerin-are-dispensable-for-antioxidant-nrf2-response-to-arsenic-and-cadmium
#20
Kazunori Hashimoto, Rima Majumdar, Yoshiaki Tsuji
Lamins are important constituents of the nuclear inner membrane and provide a platform for transcription factors and chromatin. Progerin, a C-terminal truncated lamin A mutant, causes premature aging termed Hutchinson-Gilford Progeria Syndrome (HGPS). Oxidative stress appears to be involved in the pathogenesis of HGPS, although the mechanistic role of progerin remains elusive. Here we examined whether nuclear lamins are important for a cellular antioxidant mechanism, and whether progerin compromises it. We investigated the activation of nuclear factor-E2-related factor 2 (Nrf2) which regulates various antioxidant genes including heme oxygenase-1 (HMOX1), following exposure to sodium arsenite or cadmium chloride in lamin knockdown human cell lines and primary HGPS human fibroblasts...
May 2017: Cellular Signalling
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