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https://www.readbyqxmd.com/read/29228301/targeted-inhibition-of-janus-kinases-abates-interfon-gamma-induced-invasive-behaviour-of-fibroblast-like-synoviocytes
#1
Thomas Karonitsch, Denise Beckmann, Karolina Dalwigk, Birgit Niederreiter, Paul Studenic, Ruth A Byrne, Johannes Holinka, Florian Sevelda, Adelheid Korb-Pap, Günter Steiner, Josef S Smolen, Thomas Pap, Hans P Kiener
Objectives: The aim was to explore the function of the T-cell cytokine IFNγ for mesenchymal tissue remodelling in RA and to determine whether IFNγ signalling controls the invasive potential of fibroblast-like synoviocytes (FLS). Methods: To assess architectural responses, FLS were cultured in three-dimensional micromasses. FLS motility was analysed in migration and invasion assays. Signalling events relevant to cellular motility were defined by western blots. Baricitinib and small interfering RNA pools were used to suppress Janus kinase (JAK) functions...
December 7, 2017: Rheumatology
https://www.readbyqxmd.com/read/29228113/fibroblast-growth-factor-2-and-transforming-growth-factor-beta1-oppositely-regulate-mir-221-that-targets-thrombospondin-1-in-bovine-luteal-endothelial-cells
#2
Svetlana Farberov, Rina Meidan
Thrombospondin-1 (THBS1) is an important mediator of corpus luteum (CL) regression. Highly induced during luteolysis, it acts as a natural anti-angiogenic, proapoptotic compound. THBS1 expression is regulated in bovine luteal endothelial cells (LECs) by fibroblast growth factor-2 (FGF2) and transforming growth factor-beta1 (TGFB1) acting in an opposite manner. In this study we sought to identify specific microRNAs (miRNAs) targeting THBS1 and investigate their possible involvement in FGF2 and TGFB1-mediated THBS1 expression...
December 6, 2017: Biology of Reproduction
https://www.readbyqxmd.com/read/29228018/genome-wide-association-study-identifies-a-locus-associated-with-rotator-cuff-injury
#3
Thomas R Roos, Andrew K Roos, Andrew L Avins, Marwa A Ahmed, John P Kleimeyer, Michael Fredericson, John P A Ioannidis, Jason L Dragoo, Stuart K Kim
Rotator cuff tears are common, especially in the fifth and sixth decades of life, but can also occur in the competitive athlete. Genetic differences may contribute to overall injury risk. Identifying genetic loci associated with rotator cuff injury could shed light on the etiology of this injury. We performed a genome-wide association screen using publically available data from the Research Program in Genes, Environment and Health including 8,357 cases of rotator cuff injury and 94,622 controls. We found rs71404070 to show a genome-wide significant association with rotator cuff injury with p = 2...
2017: PloS One
https://www.readbyqxmd.com/read/29227635/small-bioactive-peptides-for-biomaterials-design-and-therapeutics
#4
I W Hamley
This review is aimed to provide a concise yet extensive survey of key short bioactive peptide sequences for a range of applications ranging from biomaterials development to peptides with therapeutic uses. The following are considered: cell adhesion motifs, structural peptides, cell-penetrating and tumor-homing peptides, antimicrobial peptides, peptide hormones, growth factors and matrix metalloprotease substrates, neuropeptides, amyloid peptides, antioxidant peptides, peptide affinity tags, anticancer peptides, and others...
December 11, 2017: Chemical Reviews
https://www.readbyqxmd.com/read/29227586/identification-of-potential-crucial-genes-associated-with-carcinogenesis-of-clear-cell-renal-cell-carcinoma
#5
Erlin Song, Wenting Song, Minghua Ren, Li Xing, Wenjun Ni, Yongxiang Li, Mancheng Gong, Mingbo Zhao, Xin Ma, Xu Zhang, Ruihua An
Clear cell renal cell carcinoma (ccRCC) is a common genitourinary malignancy with high mortality. However, the molecular pathogenesis of ccRCC remains unclear and effective biomarkers for daily practice are still limited. Thus, we aimed to identify the potential crucial genes and pathways associated with carcinogenesis of ccRCC and further analyze the molecular mechanisms implicated in tumorigenesis. In the present study, expression profiles GSE 66270, GSE 53757, GSE 36895 and GSE 76351 were downloaded from GEO database, including 244 matched primary and adjacent normal tissues, furthermore, the level 3 RNAseq dataset (RNAseqV2 RSEM) of KIRC was also downloaded from The Cancer Genome Atlas (TCGA), which consist of 529 ccRCC tumors and 72 normal tissues...
December 11, 2017: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/29227193/ddx5-p68-associated-lncrna-loc284454-is-differentially-expressed-in-human-cancers-and-modulates-gene-expression
#6
Monalisa Das, Arun Renganathan, Shrinivas Nivrutti Dighe, Utsa Bhaduri, Abhijith Shettar, Geetashree Mukherjee, Paturu Kondaiah, Manchanahalli R Satyanarayana Rao
Long non-coding RNAs (lncRNAs) are emerging as important players in regulation of gene expression in higher eukaryotes. DDX5/p68 RNA helicase protein which is involved in splicing of precursor mRNAs also interacts with lncRNAs like, SRA and mrhl, to modulate gene expression. We performed RIP-seq analysis in HEK293T cells to identify the complete repertoire of DDX5/p68 interacting transcripts including 73 single exonic (SE) lncRNAs. The LOC284454 lncRNA is the second top hit of the list of SE lncRNAs which we have characterized in detail for its molecular features and cellular functions...
December 11, 2017: RNA Biology
https://www.readbyqxmd.com/read/29227167/diagnosis-of-platelet-function-disorders-a-standardized-rational-and-modular-flow-cytometric-approach
#7
Oliver Andres, Katja Henning, Gabriele Strauß, Annerose Pflug, Georgi Manukjan, Harald Schulze
A high proportion of patients with mucocutaneous bleeding diathesis and suspected inherited or acquired platelet disorder remain without diagnosis even after comprehensive laboratory testing. Since flow cytometry allows investigation of resting and activated platelets on the single cell level by requiring only minimal amounts of blood, this method has become an important assay within the diagnostic algorithm, especially in pediatrics. We therefore developed a standardized and modular flow cytometric approach that contributes to clarify impaired platelet function in a rational step-by-step manner...
December 11, 2017: Platelets
https://www.readbyqxmd.com/read/29226950/syndecans-in-chronic-inflammatory-and-autoimmune-diseases-pathological-insights-and-therapeutic-opportunities
#8
REVIEW
Solomon A Agere, Eugene Y Kim, Nahid Akhtar, Salahuddin Ahmed
Syndecans (SDCs) are a family of heparan sulfate proteoglycans (HSPGs) glycoproteins ubiquitously expressed on the cell surfaces and extracellular matrix of all mammalian tissues. There are four mammalian syndecans, SDC-1 thorough 4, which play a critical role in cell adhesion, migration, proliferation, differentiation, and angiogenesis through independent and growth factor mediated signaling. An altered expression of SDCs is often observed in autoimmune disorders, cancer, HIV infection, and many other pathological conditions...
December 11, 2017: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/29226557/lysophosphatidic-acid-accelerates-development-of-porcine-embryos-by-activating-formation-of-the-blastocoel
#9
Min-Young Shin, Seung-Eun Lee, Yeo-Jin Son, Yun-Gwi Park, Sang-Gi Jeong, Eun-Young Kim, Se-Pill Park
Culture media modifications, including the addition of various factors, are important for the in vitro production of oocytes and embryos. In this study, we investigated the effects of lysophosphatidic acid (LPA) on porcine embryo development. Porcine parthenogenetic embryos were cultured with 0, 0.1, 1, and 10 μM LPA for 7 days, or cultured in basic medium until Day 4 and then treated with LPA from Days 4 to 7. No difference in the in vitro development of embryos cultured with LPA for 7 days was observed. Conversely, rates of blastocyst and over-expanded blastocyst formation were higher in the 0...
December 11, 2017: Molecular Reproduction and Development
https://www.readbyqxmd.com/read/29226333/p53-and-mir-210-regulated-neurod2-a-neuronal-basic-helix-loop-helix-transcription-factor-is-downregulated-in-glioblastoma-patients-and-functions-as-a-tumor-suppressor-under-hypoxic-microenvironment
#10
Rahul Agrawal, Ankita Garg, Prit Benny Malgulwar, Vikas Sharma, Chitra Sarkar, Ritu Kulshreshtha
The factors involved in cell differentiation have recently garnered interest for their role in inhibition of pathogenesis in various tumors. However, their role in glioblastoma (GBM) remains poorly understood. We analyzed the TCGA-GBM data and found significant downregulation of neurogenic differentiation factor NeuroD2 in GBM patients. Low levels of NeuroD2 were found to be correlated with poor overall survival of GBM patients in TCGA dataset as well as in our cohort. Interestingly, NeuroD2 was shown to be transcriptionally induced by p53 and post-transcriptionally targeted by hypoxia inducible miRNA, miR-210...
December 11, 2017: International Journal of Cancer. Journal International du Cancer
https://www.readbyqxmd.com/read/29226271/clusters-of-circulating-tumor-cells-a-biophysical-and-technological-perspective
#11
Sam H Au, Jon Edd, Daniel A Haber, Shyamala Maheswaran, Shannon L Stott, Mehmet Toner
The vast majority of cancer associated deaths result from metastasis, yet the behaviors of its most potent cellular driver, circulating tumor cell clusters, are only beginning to be revealed. This review highlights recent advances to our understanding of tumor cell clusters with emphasis on enabling technologies. The importance of intercellular adhesions among cells in clusters have begun to be unraveled with the aid of promising microfluidic strategies for isolating clusters from patient blood. Due to their metastatic potency, the utility of circulating tumor cell clusters for cancer diagnosis, drug screening, precision oncology and as targets of antimetastatic therapeutics are being explored...
September 2017: Current Opinion in Biomedical Engineering
https://www.readbyqxmd.com/read/29226261/coordination-of-early-cellular-reactions-during-activation-of-bone-resorption-in-the-rat-mandible-periosteum-an-immunohistochemical-study
#12
Bassam Hassan, Isabelle Fouilloux, Brigitte Baroukh, Annie Llorens, Martin Biosse Duplan, Marjolaine Gosset, Marc Cherruau, Jean-Louis Saffar
The activation step of bone remodeling remains poorly characterized. Activation comprises determination of the site to be remodeled, osteoclast precursor recruitment, their migration to the site of remodeling, and differentiation. These actions involve different compartments and cell types. The aim of this study was to investigate events and cell types involved during activation. We used a bone remodeling model in rats where extractions of the upper jaw molars initiate remodeling of the antagonist lower jaw (mandible) cortex along the periosteum...
October 2017: Heliyon
https://www.readbyqxmd.com/read/29226137/identification-of-key-pathways-and-genes-in-advanced-coronary-atherosclerosis-using-bioinformatics-analysis
#13
Xiaowen Tan, Xiting Zhang, Lanlan Pan, Xiaoxuan Tian, Pengzhi Dong
Background: Coronary artery atherosclerosis is a chronic inflammatory disease. This study aimed to identify the key changes of gene expression between early and advanced carotid atherosclerotic plaque in human. Methods: Gene expression dataset GSE28829 was downloaded from Gene Expression Omnibus (GEO), including 16 advanced and 13 early stage atherosclerotic plaque samples from human carotid. Differentially expressed genes (DEGs) were analyzed. Results: 42,450 genes were obtained from the dataset...
2017: BioMed Research International
https://www.readbyqxmd.com/read/29225960/utility-of-amorphous-calcium-phosphate-based-scaffolds-in-dental-biomedical-applications
#14
Diane R Bienek, Drago Skrtic
Calcium phosphate (CaP) materials are important inorganic constituents in biological hard tissue. CaPs, including amorphous calcium phosphate (ACP) have been widely applied in dental and biomedical applications, such as tissue engineering. Scaffold constructs are commonly used as templates to create a biomimetic environment. This review considers ACP scaffold fabrication techniques, including tissue-engineered constructs with intrinsic incorporation of ACP as well as scaffolds formed via precipitation of mineralized solutions on a substrate...
2017: Biointerface Research in Applied Chemistry
https://www.readbyqxmd.com/read/29225769/fabrication-of-low-cost-micropatterned-polydimethyl-siloxane-scaffolds-to-organise-cells-in-a-variety-of-two-dimensioanl-biomimetic-arrangements-for-lab-on-chip-culture-platforms
#15
Lidia Escutia-Guadarrama, Genaro Vázquez-Victorio, David Martínez-Pastor, Brenda Nieto-Rivera, Marcela Sosa-Garrocho, Marina Macías-Silva, Mathieu Hautefeuille
We present the rapid-prototyping of type I collagen micropatterns on poly-dimethylsiloxane substrates for the biomimetic confinement of cells using the combination of a surface oxidation treatment and 3-aminopropyl triethoxysilane silanisation followed by glutaraldehyde crosslinking. The aim of surface treatment is to stabilise microcontact printing transfer of this natural extracellular matrix protein that usually wears out easily from poly-dimethylsiloxane, which is not suitable for biomimetic cell culture platforms and lab-on-chip applications...
January 2017: Journal of Tissue Engineering
https://www.readbyqxmd.com/read/29225416/getting-peek-to-stick-to-bone-the-development-of-porous-peek-for-interbody-fusion-devices
#16
F Brennan Torstrick, David L Safranski, J Kenneth Burkus, James L Chappuis, Christopher S D Lee, Robert E Guldberg, Ken Gall, Kathryn E Smith
Interbody fusion cages are routinely implanted during spinal fusion procedures to facilitate arthrodesis of a degenerated or unstable vertebral segment. Current cages are most commonly made from polyether-ether-ketone (PEEK) due to its favorable mechanical properties and imaging characteristics. However, the smooth surface of current PEEK cages may limit implant osseointegration and may inhibit successful fusion. We present the development and clinical application of the first commercially available porous PEEK fusion cage (COHERE®, Vertera, Inc...
September 2017: Techniques in Orthopaedics: TIO
https://www.readbyqxmd.com/read/29225277/evaluation-of-residual-monomer-release-and-toxicity-of-self-adhesive-resin-cements
#17
Aysegul Kurt, Subutay Han Altintas, Mustafa Volkan Kiziltas, Serife Evrim Tekkeli, Eray Metin Guler, Abdurrahim Kocyigit, Aslihan Usumez
The aim of this study was to evaluate the amount of leached residual monomers from self-adhesive resin cements and evaluate their toxicity in-vitro. A total of 60 disk-shaped specimens (5 mm in diameter and 0.5 mm in thickness) were prepared from each cement (RelyX U200, SpeedCEM, G-Cem) (n=20). Specimens were immersed in artificial saliva and the amount of released monomers [urethane dimethacrylate (UDMA) and triethyleneglycol dimethacrylate (TEGDMA)] was identified. Then, the cytotoxicity and genotoxicity effect on cells were evaluated using the defined amounts of released monomers from cements...
December 8, 2017: Dental Materials Journal
https://www.readbyqxmd.com/read/29225169/inhibition-of-orai1-mediated-ca2-entry-limits-endothelial-cell-inflammation-by-suppressing-calcineurin-nfatc4-signaling-pathway
#18
Bei-Xin Yu, Jia-Ni Yuan, Fei-Ran Zhang, Ying-Ying Liu, Ting-Ting Zhang, Kai Li, Xiao-Fei Lv, Jia-Guo Zhou, Lin-Yan Huang, Jin-Yan Shang, Si-Jia Liang
Orai1-dependent Ca2+ entry plays an essential role in inflammatory response through regulating T cell and macrophage activation and neutrophil infiltration. However, whether Orai1 Ca2+ entry contributes to endothelial activation, one of the early steps of vascular inflammation, remains elusive. In the present study, we observed that knockdown of Orai1 reduced, whereas overexpression of Orai1 potentiated, TNFα-induced expression of adhesion molecules such as ICAM-1 and VCAM-1 in HUVECs, and subsequently blocked adhesion of monocyte to HUVECs...
December 7, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29224849/aptamers-as-potential-therapeutic-agents-for-ovarian-cancer
#19
REVIEW
Justin Liam Henri, Joanna Macdonald, Mia Strom, Wei Duan, Sarah Shigdar
Current therapy for ovarian cancer typically involves indiscriminate chemotherapies that can have severe off target effects on healthy tissue and are still plagued by aggressive recurrence. Recent shifts towards targeted therapies offer the possibility of circumventing the obstacles experienced by these traditional treatments. While antibodies are the pioneering agents in targeted therapies, clinical experience has demonstrated that their antitumor efficacy is limited due to their high immunogenicity, large molecular size, and costly and laborious production...
December 7, 2017: Biochimie
https://www.readbyqxmd.com/read/29224421/hydrolysis-setting-properties-and-in%C3%A2-vitro-characterization-of-wollastonite-newberyite-bone-cement-mixtures
#20
Tibor Sopcak, Lubomir Medvecky, Maria Giretova, Radoslava Stulajterova, Juraj Durisin
Bone cements based on magnesium phosphates such as newberyite (N; MgHPO4.3H2O) have been shown as potential bone substitutes due to their biocompatibility, biodegradability and ability to support osteoblast differentiation and proliferation. Newberyite can hydrolyze to hydrated magnesium phosphate compounds (e.g. bobierite (Mg3(PO4)2.8H2O)) at alkaline conditions. In this study, 25 and 50 wt% of crystalline β -wollastonite (woll; CaSiO3) was admixed to newberyite powder in order to both enhance the acid-base hydrolysis of newberyite and to produce a functional bone cement...
January 1, 2017: Journal of Biomaterials Applications
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