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https://www.readbyqxmd.com/read/29913236/loss-of-linc01060-induces-pancreatic-cancer-progression-through-vinculin-mediated-focal-adhesion-turnover
#1
Xiuhui Shi, Xingjun Guo, Xu Li, Min Wang, Renyi Qin
There is currently limited knowledge regarding the involvement of long non-coding RNAs (lncRNAs) in cancer development. We aimed to identify lncRNAs with important roles in pancreatic cancer progression. We screened for lncRNAs that were differentially expressed in pancreatic cancer tissues. Among 349 differentially expressed lncRNAs, Linc01060 showed the lowest expression in pancreatic cancer tissues compared with normal pancreatic tissues. Lower Linc01060 expression in pancreatic cancer tissues was significantly associated with a poor prognosis...
June 15, 2018: Cancer Letters
https://www.readbyqxmd.com/read/29913212/pharmacological-inhibition-of-malt1-protease-activity-suppresses-endothelial-activation-via-enhancing-mcpip1-expression
#2
Yong Li, Shengping Huang, Xuan Huang, Xiuzhen Li, Adrian Falcon, Adele Soutar, Frederic Bornancin, Zhisheng Jiang, Hong-Bo Xin, Mingui Fu
Mucosa associated lymphoid tissue lymphoma translocation protein 1 (MALT1) is not only an intracellular signaling scaffold protein but also a paracaspase that plays a key role in the signal transduction and cellular activation of lymphocytes and macrophages. However, its role in endothelial cells remains unknown. Here we report that pharmacological inhibition of MALT1 protease activity strongly suppresses endothelial activation via enhancing MCPIP1 expression. Treatment with MALT1 protease inhibitors selectively inhibited TNFα-induced VCAM-1 expression in HUVECs and LPS-induced VCAM-1 expression in mice...
June 15, 2018: Cellular Signalling
https://www.readbyqxmd.com/read/29913095/inhibition-of-lps-induced-inflammatory-responses-by-satureja-hortensis-extracts-in-j774-1-macrophages
#3
Zahra Farzaneh, Kurosh Kalantar, Aida Iraji, Zahra Amirghofran
Macrophages are among the main cells involved in generation of inflammation. To investigate the anti-inflammatory effect of Satureja hortensis (summer savory), lipopolysaccharide (LPS)-activated J774.1 macrophages were treated with various extracts, and the expression and release of various inflammatory molecules by macrophages were examined. We showed that dichloromethane and hexane extracts reduced nitric oxide (NO) production more efficiently than other extracts. Both extracts decreased gene expression of inducible NO synthase (iNOS) (<0...
June 18, 2018: Journal of Immunoassay & Immunochemistry
https://www.readbyqxmd.com/read/29912577/glucocorticoid-exposure-causes-disrupted-glucoregulation-cardiac-inflammation-and-elevated-dipeptidyl-peptidase-4-activity-independent-of-glycogen-synthase-kinase-3-in-female-rats
#4
Olufunto O Badmus, Lawrence A Olatunji
OBJECTIVE: We tested the hypothesis that glucocorticoid (GC) exposure in female rats would lead to glucose dysregulation and elevated cardiac inflammatory biomarkers, which are dipeptidyl peptidase-4 (DPP-4)- and glycogen synthase kinase-3 (GSK-3)-dependent. METHODS: Female Wistar rats received vehicle (control; n = 6) or GC (dexamethasone; n = 6; 0.2 mg/kg; p.o.) for six days. Insulin resistance was determined by HOMA-IR. DPP-4 activity was determined by fluorescence method, whereas vascular cell adhesion molecule-1 (VCAM-1), uric acid, malondialdehyde (MDA), lactate dehydrogenase (LDH) and nitric oxide (NO) from plasma and cardiac homogenate were estimated as cardiac pro-inflammatory biomarkers...
June 18, 2018: Archives of Physiology and Biochemistry
https://www.readbyqxmd.com/read/29911975/alpha-protocadherins-and-pyk2-kinase-regulate-cortical-neuron-migration-and-cytoskeletal-dynamics-via-rac1-gtpase-and-wave-complex-in-mice
#5
Li Fan, Yichao Lu, Xiulian Shen, Hong Shao, Lun Suo, Qiang Wu
Diverse clustered protocadherins are thought to function in neurite morphogenesis and neuronal connectivity in the brain. Here we report that the protocadherin alpha ( Pcdha ) gene cluster regulates neuronal migration during cortical development and cytoskeletal dynamics in primary cortical culture through the WAVE (Wiskott-Aldrich syndrome family verprolin homologous protein, also known as WASP or Wasf) complex. In addition, overexpression of proline-rich tyrosine kinase 2 (Pyk2, also known as Ptk2b, Cakb, Raftk, Fak2, and Cadtk), a non-receptor cell-adhesion kinase and scaffold protein downstream of Pcdha, impairs cortical neuron migration via inactivation of the small GTPase Rac1...
June 18, 2018: ELife
https://www.readbyqxmd.com/read/29911385/programmable-multivalent-dna-origami-tension-probes-for-reporting-cellular-traction-forces
#6
Palash K Dutta, Yun Zhang, Aaron Blanchard, Chenghao Ge, Muaz Rushdi, Kristin Weiss, Cheng Zhu, Yonggang Ke, Khalid Salaita
Mechanical forces are central to most, if not all, biological processes including cell development, immune recognition, and metastasis. Because the cellular machinery mediating mechano-sensing and force generation is dependent on the nanoscale organization and geometry of protein assemblies, a current need in the field is the development of force-sensing probes that can be customized at the nanometer lengthscale. In this work, we describe a DNA origami tension sensor that maps the piconewton (pN) forces generated by living cells...
June 18, 2018: Nano Letters
https://www.readbyqxmd.com/read/29911340/self-assembling-nanoclay-diffusion-gels-for-bioactive-osteogenic-microenvironments
#7
Pujiang Shi, Yang-Hee Kim, Mohamed Mousa, Roxanna Ramnarine Sanchez, Richard O C Oreffo, Jonathan I Dawson
Laponite nanoparticles have attracted attention in the tissue engineering field for their protein interactions, gel-forming properties, and, more recently, osteogenic bioactivity. Despite growing interest in the osteogenic properties of Laponite, the application of Laponite colloidal gels to host the osteogenic differentiation of responsive stem cell populations remains unexplored. Here, the potential to harness the gel-forming properties of Laponite to generate injectable bioactive microenvironments for osteogenesis is demonstrated...
June 17, 2018: Advanced Healthcare Materials
https://www.readbyqxmd.com/read/29911238/adhesion-of-emulsified-oil-droplets-to-hydrophilic-and-hydrophobic-surfaces-effect-of-surfactant-charge-surfactant-concentration-and-ionic-strength
#8
Janneke M Dickhout, J Mieke Kleijn, Rob G H Lammertink, Wiebe M de Vos
Adhesion of emulsified oil droplets to a surface plays an important role in processes such as crossflow membrane filtration, where the oil causes fouling. We present a novel technique, in which we study oil droplets on a model surface in a flow cell under shear force to determine the adhesive force between droplets and surface. We prepared an emulsion of hexadecane and used hydrophilic and hydrophobic glass slides as model surfaces. Different surfactants were used as emulsifiers: negatively charged sodium dodecyl sulphate (SDS), positively charged hexadecyltrimethylammonium bromide (CTAB) and nonionic Triton X-100...
June 18, 2018: Soft Matter
https://www.readbyqxmd.com/read/29910738/immunomodulatory-effects-of-dietary-seaweeds-in-lps-challenged-atlantic-salmon-salmo-salar-as-determined-by-deep-rna-sequencing-of-the-head-kidney-transcriptome
#9
Arjan P Palstra, Jeroen Kals, Ainhoa Blanco Garcia, Ron P Dirks, Marnix Poelman
Seaweeds may represent immuno-stimulants that could be used as health-promoting fish feed components. This study was performed to gain insights into the immunomodulatory effects of dietary seaweeds in Atlantic salmon. Specifically tested were 10% inclusion levels of Laminaria digitata (SW1) and a commercial blend of seaweeds (Oceanfeed® ) (SW2) against a fishmeal based control diet (FMC). Differences between groups were assessed in growth, feed conversion ratio and blood parameters hematocrit and hemoglobin...
2018: Frontiers in Physiology
https://www.readbyqxmd.com/read/29910635/impact-of-semaphorin-expression-on-prognostic-characteristics-in-breast-cancer
#10
REVIEW
Ramesh Butti, Totakura Vs Kumar, Ramakrishna Nimma, Gopal C Kundu
Breast cancer is one of the major causes of cancer-related deaths among women worldwide. Aberrant regulation of various growth factors, cytokines, and other proteins and their receptors in cancer cells drives the activation of various oncogenic signaling pathways that lead to cancer progression. Semaphorins are a class of proteins which are differentially expressed in various types of cancer including breast cancer. Earlier, these proteins were known to have a major function in the nerve cell adhesion, migration, and development of the central nervous system...
2018: Breast Cancer: Targets and Therapy
https://www.readbyqxmd.com/read/29910565/proteomic-analysis-of-avian-influenza-a-h7n9-patients-within-a-family-cluster
#11
Yang Zheng, Xiaomin Lou, Peng Yang, Weixian Shi, Yanda Chu, Meishuang Yan, Cuicui Jiang, Di Wu, Yang Pan, Jiachen Zhao, Yang Li, Yusheng Dong, Lijuan Chen, Siqi Liu, Quanyi Wang
Background: To date, there is limited information on the progression of human infections of avian influenza virus A (H7N9). This study investigated differential blood protein profiling of a H7N9-infected family cluster to find a slice of crucial proteins concerning disease attack and virus clearance. Materials and Methods: Plasma samples from one family cluster (including one index case and one asymptomatic case) were collected at four time points. The protein profiles were identified by isobaric tagging for relative and absolute quantification-based quantitative differential LC/MS/MS, and their functional annotations were analyzed by PANTHER and STRING tools...
April 2018: Journal of Global Infectious Diseases
https://www.readbyqxmd.com/read/29910080/synthesis-and-biological-evaluation-of-pyridine-linked-indanone-derivatives-potential-agents-for-inflammatory-bowel-disease
#12
Tara Man Kadayat, Suhrid Banskota, Ganesh Bist, Pallavi Gurung, Til Bahadur Thapa Magar, Aarajana Shrestha, Jung-Ae Kim, Eung-Seok Lee
A series of pyridine-linked indanone derivatives were designed and synthesized to discover new small molecules for the treatment of inflammatory bowel disease (IBD). Compounds 5b and 5d exhibited strongest inhibitory activity against TNF-α-induced monocyte adhesion to colon epithelial cells (an in vitro model of colitis). In TNBS (2,4,6-trinitrobenzenesulfonic acid)-induced rat colitis model, oral administration of the compounds 5b and 5d ameliorated colitis with significant recovery in altered expressions of E-cadherin, TNF-α and IL-1β expressions, indicating 5b and 5d as potential agents for therapeutics development against IBD...
June 8, 2018: Bioorganic & Medicinal Chemistry Letters
https://www.readbyqxmd.com/read/29910076/focal-adhesions-undergo-longitudinal-splitting-into-fixed-width-units
#13
Lorna E Young, Henry N Higgs
Focal adhesions (FAs) and stress fibers (SFs) act in concert during cell motility and in response to the extracellular environment. Although the structures of mature FAs and SFs are well studied, less is known about how they assemble and mature de novo during initial cell spreading. In this study using live-cell Airyscan microscopy, we find that FAs undergo "splitting" during their assembly, in which the FA divides along its longitudinal axis. Before splitting, FAs initially appear as assemblies of multiple linear units (FAUs) of 0...
May 25, 2018: Current Biology: CB
https://www.readbyqxmd.com/read/29909601/assessment-of-the-dual-role-of-clumping-factor-a-in-s-aureus-adhesion-to-endothelium-in-absence-and-presence-of-plasma
#14
Jorien Claes, Bartosz Ditkowski, Laurens Liesenborghs, Tiago Rafael Veloso, Jose M Entenza, Philippe Moreillon, Thomas Vanassche, Peter Verhamme, Marc F Hoylaerts, Ruth Heying
Adhesion of Staphylococcus aureus to endothelial cells (ECs) is paramount in infective endocarditis. Bacterial proteins such as clumping factor A (ClfA) and fibronectin binding protein A (FnbpA) mediate adhesion to EC surface molecules and (sub)endothelial matrix proteins including fibrinogen (Fg), fibrin, fibronectin (Fn) and von Willebrand factor (vWF). We studied the influence of shear flow and plasma on the binding of ClfA and FnbpA (including its sub-domains A, A16+ , ABC, CD) to coverslip-coated vWF, Fg/fibrin, Fn or confluent ECs, making use of Lactococcus lactis , expressing these adhesins heterologously...
June 17, 2018: Thrombosis and Haemostasis
https://www.readbyqxmd.com/read/29909439/a-patterned-polystyrene-based-microelectrode-array-for-in-vitro-neuronal-recordings
#15
Audrey Hammack, Rashed T Rihani, Bryan J Black, Joseph J Pancrazio, Bruce E Gnade
Substrate-integrated microelectrode arrays (MEAs) are non-invasive platforms for recording supra-threshold signals, i.e. action potentials or spikes, from a variety of cultured electrically active cells, and are useful for pharmacological and toxicological studies. However, the MEA substrate, which is often fabricated using semiconductor processing technology, presents some challenges to the user. Specifically, the electrode encapsulation, which may consist of a variety of inorganic and organic materials, requires a specific substrate preparation protocol to optimize cell adhesion to the surface...
June 16, 2018: Biomedical Microdevices
https://www.readbyqxmd.com/read/29909342/fak-and-bmp-9-synergistically-trigger-osteogenic-differentiation-and-bone-formation-of-adipose-derived-stem-cells-through-enhancing-wnt-%C3%AE-catenin-signaling
#16
Cheng Yuan, Xiaoli Gou, Jiang Deng, Zhijun Dong, Peng Ye, Zhenming Hu
BACKGROUNDS: Adipose derived stem cells (ADSCs) could undergo osteogenesis via focal adhesion kinase (FAK) and bone morphogenetic protein (BMP) 9 signals, both of which could affect Wnt-β-catenin signal, a signal pathway closely related to ADSCs osteogenesis. It's still enigma whether FAK and BMP-9 contribute to osteogenesis. Here, we examined the effect of FAK on BMP9-inducedosteogenic differentiation, unveiled the possible molecular mechanism underling this process. METHODS: In the present study, ADSCs were isolated and purified, and cells of passage 3 underwent virus mediated transfection to prepare ADSCs with stable FAK shRNA expression...
June 14, 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/29909279/the-kank-family-proteins-in-adhesion-dynamics
#17
REVIEW
Nan-Peng Chen, Zhiqi Sun, Reinhard Fässler
Integrin-mediated cell adhesion plays key roles for cell movement during development and tissue homeostasis. The dynamic life cycle of various integrin adhesions structures is required for the cell movements and regulated by the coordinated actions of both actomyosin and the microtubule (MT) cytoskeleton. The evolutionarily conserved Kank family proteins have emerged as regulators of adhesion dynamics by coordinating integrin-mediated force transmission with the recruitment of microtubules to integrins. These novel functions may play important roles in vivo and in human diseases...
June 14, 2018: Current Opinion in Cell Biology
https://www.readbyqxmd.com/read/29909274/molecular-and-functional-analyses-of-novel-anti-lipopolysaccharide-factors-in-giant-river-prawn-macrobrachium-rosenbergii-de-man-and-their-expression-responses-under-pathogen-and-temperature-exposure
#18
Prapansak Srisapoome, Nawanith Klongklaew, Nontawith Areechon, Ratree Wongpanya
Anti-lipopolysaccharide factor (ALF) is an immune-related protein that is crucially involved in immune defense mechanisms against invading pathogens in crustaceans. In the current study, three different ALFs of giant river prawn (Mr-ALF3, Mr-ALF8 and Mr-ALF9) were discovered. Based on sequence analysis, Mr-ALF3 and Mr-ALF9 were identified as new members of ALFs in crustaceans (groups F and G, respectively). Structurally, each newly identified Mr-ALF contained three α-helices packed against a four-stranded β-sheet bearing the LPS-binding motif, which usually binds to the cell wall components of bacteria...
June 14, 2018: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/29909209/circular-rna-in-invasive-and-recurrent-clinical-nonfunctioning-pituitary-adenomas-expression-profiles-and-bioinformatic-analysis
#19
Jianpeng Wang, Dong Wang, Dehong Wan, Qingxia Ma, Qian Liu, Jiye Li, Zhaojian Li, Yang Gao, Guohui Jiang, Leina Ma, Jia Liu, Chuzhong Li
BACKGROUND: The invasion and recurrence of clinical nonfunctioning pituitary adenomas (NFA) often lead to surgical treatment failure. Circular RNAs (circRNAs) are a novel class of RNAs whose 3' and 5' ends are joined together and have been shown to play important roles in cancer development. Up to now, the roles of circRNAs remain unclear in invasive and recurrent NFA. METHODS: We detected and summarized the circRNA expression pattern in 75 NFA tissues from 10 non-invasive cases and 65 invasive cases and 9 pairs NFA tumor tissues from 9 recurrent cases by circRNA microarrays...
June 14, 2018: World Neurosurgery
https://www.readbyqxmd.com/read/29909133/tuberculin-skin-test-reaction-is-related-to-memory-but-not-naive-cd4-t-cell-responses-to-mycobacterial-stimuli-in-bcg-vaccinated-young-adults
#20
Magdalena Kowalewicz-Kulbat, Piotr Szpakowski, Camille Locht, Franck Biet, Paulina Kaplonek, Krzysztof T Krawczyk, Joël Pestel, Wieslawa Rudnicka
Bacillus Calmette-Guérin (BCG) is the only vaccine available against tuberculosis and the tuberculin skin test (TST) is the most widely used method to detect BCG take. However, subjects may remain TST-negative, even after several BCG administrations. To investigate some of the potential reasons underlying this inability of developing tuberculin sensitivity in response to BCG we compared the effect of different mycobacterial stimuli in the groups differently responding to tuberculin. TST was performed on 71 healthy adults aged 25-30 years, who had received BCG in their childhood, and considered TST-positive at ≥10 mm...
June 13, 2018: Vaccine
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