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https://www.readbyqxmd.com/read/29668032/presence-of-meniscus-tear-alters-gene-expression-profile-of-anterior-cruciate-ligament-tears
#1
Robert H Brophy, Marcus A Rothermich, Eric D Tycksen, Lei Cai, Muhammad Farooq Rai
Anterior cruciate ligament (ACL) tears occur in isolation or in tandem with other intra-articular injuries such as meniscus tears. The impact of injury pattern on the molecular biology of the injured ACL is unknown. Here, we tested the hypothesis that the biological response of the ACL to injury varies based on the presence or absence of concomitant meniscus tear. We performed RNA-seq on 28 ACL tears remnants (12 isolated, 16 combined). In total, 16,654 transcripts were differentially-expressed between isolated and combined injury groups at false-discovery-rate of 0...
April 18, 2018: Journal of Orthopaedic Research: Official Publication of the Orthopaedic Research Society
https://www.readbyqxmd.com/read/29667532/human-osteoblast-derived-extracellular-matrix-with-high-homology-to-bone-proteome-is-osteopromotive
#2
Marta Baroncelli, Bram C J van der Eerden, Siddharth Chatterji, Enrique Rull Trinidad, Yik Y Kan, Marijke Koedam, Ingmar A J van Hengel, Rodrigo D A M Alves, Lidy E Fratila-Apachitei, Jeroen A A Demmers, Jeroen van de Peppel, Johannes Ptm van Leeuwen
Efficient osteogenic differentiation of mesenchymal stem cells (MSCs) is crucial to accelerate bone formation. In this context, the use of extracellular matrix (ECM) as natural 3D-framework mimicking in vivo tissue architecture is of interest. The aim of this study was to generate a devitalized human osteogenic MSC-derived ECM and to investigate its impact on MSC osteogenic differentiation to improve MSC properties in bone regeneration. The devitalized ECM significantly enhanced MSC adhesion and proliferation...
April 18, 2018: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/29667344/concise-review-aggressive-colorectal-cancer-role-of-epithelial-cell-adhesion-molecule-in-cancer-stem-cells-and-epithelial-to-mesenchymal-transition
#3
REVIEW
Maximilian Boesch, Gilbert Spizzo, Andreas Seeber
Colorectal cancer (CRC) is one of the most common malignancies worldwide. In spite of various attempts to ameliorate outcome by escalating treatment, significant improvement is lacking particularly in the adjuvant setting. It has been proposed that cancer stem cells (CSCs) and the epithelial-to-mesenchymal transition (EMT) are at least partially responsible for therapy resistance in CRC. The epithelial cell adhesion molecule (EpCAM) was one of the first CSC antigens to be described. Furthermore, an EpCAM-specific antibody (edrecolomab) has the merit of having launched the era of monoclonal antibody treatment in oncology in the 1990s...
April 17, 2018: Stem Cells Translational Medicine
https://www.readbyqxmd.com/read/29667205/application-of-heparin-collagen-redv-selective-active-interface-on-eptfe-films-to-enhance-endothelialization-and-anticoagulation
#4
Yaping Shan, Bing Jia, Ming Ye, Hua Shen, Weicheng Chen, Huifeng Zhang
Expanded polytetrafluoroethylene (ePTFE) prosthetic valves have been widely used in clinical applications in Asian countries. However, these valves still have limits with regard to thrombosis, neointimal hyperplasia, restenosis, and valvar vegetation. The achievement of in situ endothelialization on implant materials is a promising way to overcome those limits. Here, heparin/collagen multilayers were fabricated on ePTFE films via a layer-by-layer (LBL) self-assembly technique, and then, the endothelial cell (EC) adhesive peptide sequence Arg-Glu-Asp-Val (REDV) was immobilized on the multilayers...
April 18, 2018: Artificial Organs
https://www.readbyqxmd.com/read/29667009/encapsulating-viability-of-multi-strain-lactobacilli-as-potential-probiotic-in-pigs
#5
Sudthidol Piyadeatsoontorn, Rutjawate Taharnklaew, Tewa Upathanpreecha, Pairat Sornplang
Important aspects of the selection of probiotics to be used for mixing in animal feed include host species specificity and probiotic cell survival during production and storage of their products. The research was to screen and investigate some probiotic properties of lactic acid bacteria (LAB) isolated from pig fecal samples. One hundred and thirty-eight representative LAB isolates, which were isolated from 51 pig fecal samples, were tested for acid and bile tolerance, antimicrobial susceptibility, antibacterial activity, potential adhesion to the cell surface, and survival rates when stored in varied microencapsulation forms: freeze-dried, spray-dried, and micro-beads...
April 17, 2018: Probiotics and Antimicrobial Proteins
https://www.readbyqxmd.com/read/29666866/dependence-of-cell-adhesion-on-extracellular-matrix-materials-formed-on-pore-bridge-boundaries-by-nanopore-opening-and-closing-geometry
#6
Sueon Kim, Dong Yeol Han, Zhenzhong Chen, Won Gu Lee
In this study, we report experimental results for characterization of the growth and formation of pore bridge materials that modified the adhesion structures of cells cultured on nanomembranes with opening and closing geometry. To perform the proof-of-concept experiments, we fabricated two types of anodized alumina oxide substrates with single-sided opening (i.e., one side open, but closed at the other side) and double-sided opening (i.e., both sides open). In our experiment, we compared the densities of pores formed and of bridge materials which differently act as connective proteins depending on the size of pores...
April 18, 2018: Analyst
https://www.readbyqxmd.com/read/29666857/chitosan-catechol-a-writable-bioink-under-serum-culture-media
#7
Daiheon Lee, Joseph P Park, Mi-Young Koh, Pureum Kim, Junhee Lee, Mikyung Shin, Haeshin Lee
Mussel-inspired adhesive coatings on biomedical devices have attracted significant interest due to their unique properties such as substrate independency and high efficiency. The key molecules for mussel-inspired adhesive coatings are catechol and amine groups. Along with the understanding of catechol chemistry, chitosan-catechol has also been developed as a representative mussel-inpired adhesive polymer that contains catechol and amine groups for adhesiveness. Herein, we demonstrated the direct writability of chitosan-catechol as a bioink for 3D printing, one of the additive techniques...
April 18, 2018: Biomaterials Science
https://www.readbyqxmd.com/read/29666253/biomaterial-surface-energy-driven-ligand-assembly-strongly-regulates-stem-cell-mechanosensitivity-and-fate-on-very-soft-substrates
#8
Tojo Razafiarison, Claude N Holenstein, Tino Stauber, Milan Jovic, Edward Vertudes, Marko Loparic, Maciej Kawecki, Laetitia Bernard, Unai Silvan, Jess G Snedeker
Although mechanisms of cell-material interaction and cellular mechanotransduction are increasingly understood, the mechanical insensitivity of mesenchymal cells to certain soft amorphous biomaterial substrates has remained largely unexplained. We reveal that surface energy-driven supramolecular ligand assembly can regulate mesenchymal stem cell (MSC) sensing of substrate mechanical compliance and subsequent cell fate. Human MSCs were cultured on collagen-coated hydrophobic polydimethylsiloxane (PDMS) and hydrophilic polyethylene-oxide-PDMS (PEO-PDMS) of a range of stiffnesses...
April 16, 2018: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/29666211/drivers-of-persistent-infection-pathogen-induced-extracellular-vesicles
#9
REVIEW
Michael J Cipriano, Stephen L Hajduk
Extracellular vesicles (EVs) are produced by invading pathogens and also by host cells in response to infection. The origin, composition, and function of EVs made during infection are diverse and provide effective vehicles for localized and broad dissimilation of effector molecules in the infected host. Extracellular pathogens use EVs to communicate with each other by sensing the host environment contributing to social motility, tissue tropism, and persistence of infection. Pathogen-derived EVs can also interact with host cells to influence the adhesive properties of host membranes and to alter immune recognition and response...
April 17, 2018: Essays in Biochemistry
https://www.readbyqxmd.com/read/29665935/-prokaryotic-expression-and-purification-of-the-notch-ligand-and-its-effect-on-hematopoiesis-after-carbon-tetrachloride-damage
#10
Juan-Juan Chen, Si-Yong Huang, Peng-Fei Ma, Bi-Jia Wu, Si-Lang Zhou, Yong-Xing Zhao, Jun-Mei Gong, Ying-Min Liang
OBJECTIVE: To express and purify the mouse endothelial cell-targeted recombinant Notch ligand protein mD1R, and to investigate its effect on hematopoiesis after carbon tetrachloride damage. METHODS: PCR was performed to clone and construct the expression vector pET22b(+)-mD1R. The mD1R successfully transformed into E. coli was induced by IPTG, and purified with Ni2+ -beads affinity chromatography. The target protein was detected by SDS-PAGE. The fluorescence-activated cell sorting analysis (FACS), cell adhesion test, immunofluorescence staining and quantitative real-time PCR were employed to detect the endothelial cell-targeted and Notch signaling-activated biological characteristics of mD1R...
April 2018: Zhongguo Shi Yan Xue Ye Xue za Zhi
https://www.readbyqxmd.com/read/29665275/effect-of-edta-and-hyaluronic-acid-on-the-viability-and-cytokine-expression-of-periodontal-ligament-fibroblasts
#11
Monique Costa Moreira França, Tânia Mara Da Silva, Gleyce Oliveira Silva, Márcia Carneiro Valera, Carlos Henrique Ribeiro Camargo
BACKGROUND/AIMS: Dental root cell proliferation following tooth avulsion has not been well researched. Understanding the effects of dry time and dentin treatment influences on cell proliferation is essential to provide evidence-based guidelines for tooth replantation. The study evaluated the viability of periodontal ligament fibroblasts (PLF) in contact with roots, submitted to surface treatments with EDTA and hyaluronic acid (HA) at different times, including to quantify inflammatory cytokines IL-6, IL-8, IL-1β and TNF-α expressed by PLF...
April 17, 2018: Dental Traumatology: Official Publication of International Association for Dental Traumatology
https://www.readbyqxmd.com/read/29665169/fes-2-nanoparticles-decorated-graphene-as-microbial-fuel-cell-anode-achieving-high-power-density
#12
Ruiwen Wang, Mei Yan, Huidong Li, Lu Zhang, Benqi Peng, Jinzhi Sun, Da Liu, Shaoqin Liu
Microbial fuel cells (MFCs) have received great attention worldwide due to their potential in recovering electrical energy from waste and inexhaustible biomass. Unfortunately, the difficulty of achieving the high power, especially in real samples, remains a bottleneck for their practical applications. Herein, FeS2 nanoparticles decorated graphene is fabricated via a simple hydrothermal reaction. The FeS2 nanoparticles decorated graphene anode not only benefits bacterial adhesion and enrichment of electrochemically active Geobacter species on the electrode surface but also promotes efficient extracellular electron transfer, thus giving rise to a fast start-up time of 2 d, an unprecedented power density of 3220 mW m-2 and a remarkable current density of 3...
April 17, 2018: Advanced Materials
https://www.readbyqxmd.com/read/29664599/bioinspired-materials-for-controlling-ice-nucleation-growth-and-recrystallization
#13
Zhiyuan He, Kai Liu, Jianjun Wang
Ice formation, mainly consisting of ice nucleation, ice growth, and ice recrystallization, is ubiquitous and crucial in wide-ranging fields from cryobiology to atmospheric physics. Despite active research for more than a century, the mechanism of ice formation is still far from satisfactory. Meanwhile, nature has unique ways of controlling ice formation and can provide resourceful avenues to unravel the mechanism of ice formation. For instance, antifreeze proteins (AFPs) protect living organisms from freezing damage via controlling ice formation, for example, tuning ice nucleation, shaping ice crystals, and inhibiting ice growth and recrystallization...
April 17, 2018: Accounts of Chemical Research
https://www.readbyqxmd.com/read/29664201/rare-blood-donor-needed
#14
Richard R Gammon, Harold Alvarez, Nancy Benitez
BACKGROUND: Here, we describe a 14-year-old male with leukocyte adhesion deficiency type 2 who was transferred to a university hospital with anemia (hemoglobin 6 g/dL) and multiple singular abscesses refractory to antimicrobials. CASE REPORT: As leukocyte adhesion deficiency type 2 is associated with Bombay phenotype, the patient's red blood cells (RBCs) were tested with commercial anti-H lectin Ulex europaeus. An allogeneic adsorption with phenotype-matched cells was performed...
April 17, 2018: Transfusion
https://www.readbyqxmd.com/read/29663725/quartz-crystal-microbalance-as-cell-based-biosensor-to-detect-and-study-cytoskeletal-alterations-and-dynamics
#15
Monica Bianco, Daniele Vergara, Stefania De Domenico, Michele Maffia, Antonio Gaballo, Valentina Arima
Several techniques can be used to monitor cell dynamism after a perturbation. Among these, Quartz Crystal Microbalance with Dissipation Monitoring (QCM-D) offers the great advantage to study the mechanical properties of cells in real-time and with a great sensitivity. Here, we used QCM-D to investigate the effects of two cytoskeleton-targeting agents, cytochalasin D (CytoD) and Y27632, on human MCF-7 cells. Cell adhesion on the sensor surface, crucial for in-flow experiments, was obtained by covalent adsorption of a fibronectin (FN) film, an extracellular matrix (ECM) protein...
April 16, 2018: Biotechnology Journal
https://www.readbyqxmd.com/read/29663651/engineered-natural-and-synthetic-polymer-surfaces-induce-nuclear-deformation-in-osteosarcoma-cells
#16
Ezgi Antmen, Menekse Ermis, Utkan Demirci, Vasif Hasirci
Cell-substrate interactions involve constant probing of microenvironment by cells. One of the responses of cells to environmental cues is to change the conformation of their cytoplasm and nucleus. We hypothesized that surface chemistry and topography could be engineered to make these differences significant enough. When designing the substrates that would accentuate these differences, we prepared surfaces carrying cell adhesive biological cues arranged in specific patterns. Collagen type I and poly(lactic acid-co-glycolic acid) (PLGA) were used to represent substrates with biological cues and those without, and these materials were decorated with four square prism micropillars with different dimensions...
April 16, 2018: Journal of Biomedical Materials Research. Part B, Applied Biomaterials
https://www.readbyqxmd.com/read/29662977/handheld-skin-printer-in-situ-formation-of-planar-biomaterials-and-tissues
#17
Navid Hakimi, Richard Cheng, Lian Leng, Mohammad Sotoudehfar, Phoenix Qing Ba, Nazihah Bakhtyar, Saeid Amini-Nik, Marc G Jeschke, Axel Günther
We present a handheld skin printer that enables the in situ formation of biomaterial and skin tissue sheets of different homogeneous and architected compositions. When manually positioned above a target surface, the compact instrument (weight <0.8 kg) conformally deposits a biomaterial or tissue sheet from a microfluidic cartridge. Consistent sheet formation is achieved by coordinating the flow rates at which bioink and cross-linker solution are delivered, with the speed at which a pair of rollers actively translate the cartridge along the surface...
April 17, 2018: Lab on a Chip
https://www.readbyqxmd.com/read/29662904/identification-of-differentially-expressed-genes-in-porcine-ovaries-at-proestrus-and-estrus-stages-using-rna-seq-technique
#18
Songbai Yang, Xiaolong Zhou, Yue Pei, Han Wang, Ke He, Ayong Zhao
Estrus is an important factor for the fecundity of sows, and it is involved in ovulation and hormone secretion in ovaries. To better understand the molecular mechanisms of porcine estrus, the expression patterns of ovarian mRNA at proestrus and estrus stages were analyzed using RNA sequencing technology. A total of 2,167 differentially expressed genes (DEGs) were identified ( P ≤ 0.05, |log2   Ratio| ≥ 1), of which 784 were upregulated and 1,383 were downregulated in the estrus compared with the proestrus group...
2018: BioMed Research International
https://www.readbyqxmd.com/read/29662864/expression-of-adhesion-molecules-on-cd34-cells-from-steady-state-bone-marrow-before-and-after-mobilization-and-their-association-with-the-yield-of-cd34-cells
#19
Karin Zattar Cecyn, Eliza Y S Kimura, Dulce Marta S M Lima, Miyoko Yamamoto, José Orlando Bordin, José Salvador R de Oliveira
Background: Cell adhesion molecules (CAMs) expressed on hematopoietic progenitor cells (HPCs), endothelial cells, and stromal cells play a pivotal role in the mobilization of CD34+ cells. Herein, we conducted a non-randomized peripheral blood stem cell (PBSC) mobilization study aimed to compare the potential differences in the expressions of several CAMs and chemokines on CD34+ cells obtained from bone marrow aspirate before and after HPC mobilization from patients with hematologic malignancies and healthy donors...
March 2018: Blood Research
https://www.readbyqxmd.com/read/29662790/update-on-biomaterials-for-prevention-of-epidural-adhesion-after-lumbar-laminectomy
#20
REVIEW
Huailan Wang, Wenjia Sun, Dongliang Fu, Yueliang Shen, Ying-Ying Chen, Lin-Lin Wang
Lumbar laminectomy often results in failed back surgery syndrome. Most scholars support the three-dimensional theory of adhesion: Fibrosis surrounding the epidural tissues is based on the injured sacrospinalis behind, fibrous rings and posterior longitudinal ligaments. Approaches including using the minimally invasive technique, drugs, biomaterial and nonbiomaterial barriers to prevent the postoperative epidural adhesion were intensively investigated. Nevertheless, the results are far from satisfactory. Our review is based on various implant biomaterials that are used in clinical applications or are under study...
April 2018: Journal of Orthopaedic Translation
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