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https://www.readbyqxmd.com/read/28527427/photothermal-therapy-using-folate-conjugated-gold-nanoparticles-enhances-the-effects-of-6mv-x-ray-on-mouth-epidermal-carcinoma-cells
#1
Ali Neshastehriz, Mousa Tabei, Shayan Maleki, Samira Eynali, Ali Shakeri-Zadeh
The aim of this study was to develop an optimized method for preparation of folate conjugated gold nanoparticles (F-AuNPs) and to investigate its cytotoxic effects and cell apoptosis in combination with photothermal therapy (PTT) and radiotherapy (RT) for the treatment of mouth epidermal carcinoma cells KB. For this purpose, cells were treated with synthesized F-AuNPs at different concentrations for 6h and then irradiated them with laser beam (532nm, 0.5W/cm(2), 15min). After photothermal therapy, the cells were exposed to 6MV X-ray with a single dose of 2Gy...
May 12, 2017: Journal of Photochemistry and Photobiology. B, Biology
https://www.readbyqxmd.com/read/28527426/malvidin-and-cyanidin-derivatives-from-a%C3%A3-ai-fruit-euterpe-oleracea-mart-counteract-uv-a-induced-oxidative-stress-in-immortalized-fibroblasts
#2
Ganna Petruk, Anna Illiano, Rita Del Giudice, Assunta Raiola, Angela Amoresano, Maria Manuela Rigano, Renata Piccoli, Daria Maria Monti
UV-A radiations are known to induce cellular oxidative stress, leading to premature skin aging. Consumption of açai fruit (Euterpe oleracea Martius) is known to have many health benefits due to its high level of antioxidants. Herein, we analyzed the ability of phenolic compounds extracted from this fruit to attenuate UV-A-induced oxidative stress in immortalized fibroblast. A methanol/water açai extract was fractionated by HPLC and each fraction tested for anti-oxidant stress activity. Immortalized fibroblasts were pre-incubated with açai fractions and then exposed to UV-A radiations...
May 12, 2017: Journal of Photochemistry and Photobiology. B, Biology
https://www.readbyqxmd.com/read/28527371/lutein-exerts-anti-inflammatory-effects-in-patients-with-coronary-artery-disease
#3
Rosanna W S Chung, Per Leanderson, Anna K Lundberg, Lena Jonasson
BACKGROUND AND AIMS: Many coronary artery disease (CAD) patients exhibit chronic low-grade inflammation. Carotenoids are anti-oxidants with potential anti-inflammatory properties. Here, we first assessed relationships between interleukin (IL)-6 and individual carotenoids in plasma from CAD patients. Based on the results, we proceeded to assess anti-inflammatory effects of one carotenoid, lutein, in peripheral blood mononuclear cells (PBMCs) from CAD patients. METHODS: Lutein + zeaxanthin (isomers with lutein being dominant), β-cryptoxanthin, lycopene, α- and β-carotene and IL-6 were measured in plasma from 134 patients with stable angina (SA) and 59 patients with acute coronary syndrome...
May 6, 2017: Atherosclerosis
https://www.readbyqxmd.com/read/28527322/arsenic-biotransformation-by-a-cyanobacterium-nostoc-sp-pcc-7120
#4
Xi-Mei Xue, Yu Yan, Chan Xiong, Georg Raber, Kevin Francesconi, Ting Pan, Jun Ye, Yong-Guan Zhu
Nostoc sp. PCC 7120 (Nostoc), a typical filamentous cyanobacterium ubiquitous in aquatic system, is recognized as a model organism to study prokaryotic cell differentiation and nitrogen fixation. In this study, Nostoc cells incubated with arsenite (As(III)) for two weeks were extracted with dichloromethane/methanol (DCM/MeOH) and the extract was partitioned between water and DCM. Arsenic species in aqueous and DCM layers were determined using high performance liquid chromatography - inductively coupled plasma mass spectrometer/electrospray tandem mass spectrometry (HPLC-ICPMS/ESIMSMS)...
May 17, 2017: Environmental Pollution
https://www.readbyqxmd.com/read/28527286/-possible-oral-transmission-of-chagas-disease-among-hydrocarbons-sector-workers-in-casanare-colombia-2014
#5
Liliana Patricia Zuleta-Dueñas, Ángela Johana López-Quiroga, Fernando Torres-Torres, Oneida Castañeda-Porras
INTRODUCTION: Trypanosoma cruzi, the etiological agent for Chagas disease, can be transmitted by oral intake of contaminated food or drinks. During epidemiological week 14 of 2014, two cases of acute Chagas disease were notified among hydrocarbons sector workers in Paz de Ariporo, Casanare. OBJECTIVE: To characterize the affected population, to establish control and prevention measures and to confirm the outbreak. MATERIALS AND METHODS: We conducted an outbreak investigation that included the following components: a) Search for symptomatic people compatible with Chagas disease according to the case definition for their referral to medical services; b) entomological survey (192/197 houses); c) sanitary inspection and microbiological analysis of food samples; and d) study of reservoirs...
June 1, 2017: Biomédica: Revista del Instituto Nacional de Salud
https://www.readbyqxmd.com/read/28527143/characterization-of-arsenic-availability-in-dry-and-flooded-soils-using-sequential-extraction-and-diffusive-gradients-in-thin-films-dgt-techniques
#6
Liping Zhang, Qin Sun, Shiming Ding, Xiang Cheng, Qin Liu, Chaosheng Zhang
Much attention has been paid to the availability of arsenic (As) in soils, while few studies were carried out on the comparison between the dry and flooded soils using different methods. In this study, chemical sequential extraction and diffusive gradients in thin films (DGT) techniques were employed to study the availability of As in soils amended with a range of exogenous As followed by one-year incubation under dry and flooded conditions, respectively. The results showed that the proportions of available solid As pools, including non-specifically adsorbed As (F1) and specifically adsorbed As (F2), had consistent increases with the increase of As amendment...
May 19, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28527140/effect-of-tannery-effluent-on-oxidative-status-of-brain-structures-and-liver-of-rodents
#7
Felipe Dos Santos Moysés, Karine Bertoldi, Viviane Rostirola Elsner, Laura Reck Cechinel, Carla Basso, Simone Stülp, Marco Antônio Siqueira Rodrigues, Ionara Rodrigues Siqueira
Oxidative stress has been considered as a central mechanism of toxicity induced by xenobiotics. Previously, it was demonstrated that mice exposed to tannery effluent showed an anxiety-like behavior, without any comparable behavioral effects in rats. The aim of the present study was to investigate the impact of tannery wastewater on oxidative status in in vitro and in vivo assays with two mammal species, mice and rats. Specifically, homogenates of two brain areas and the liver were incubated with tannery wastewater; reactive species and lipid peroxidation levels and antioxidant enzyme activities were detected...
May 19, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28526951/pyrocincholic-acid-3%C3%AE-o-%C3%AE-d-quinovopyranosyl-28-o-%C3%AE-d-glucopyranoside-suppresses-adipogenesis-and-regulates-lipid-metabolism-in-3t3-l1-adipocytes
#8
Li Peng, Yanting Lu, Yuhui Xu, Jing Hu, Fang Wang, Yumei Zhang, Wenyong Xiong
Obesity is crucially involved in many metabolic diseases, such as type 2 diabetes, cardiovascular disease and cancer. Regulating the number or size of adipocytes has been suggested to be a potential treatment for obesity. In this study, we investigated the effect of pyrocincholic acid 3β-O-β-D-quinovopyranosyl-28-O-β-D-glucopyranoside (PAQG), a 27-nor-oleanolic acid saponin extracted from Metadina trichotoma, on adipogenesis and lipid metabolism in 3T3-L1 adipocytes. The 3T3-L1 pre-adipocytes were incubated with vehicle or PAQG for 6 days in differentiation process...
May 19, 2017: Natural Products and Bioprospecting
https://www.readbyqxmd.com/read/28526934/exogenous-proline-stimulates-type-i-collagen-and-hif-1%C3%AE-expression-and-the-process-is-attenuated-by-glutamine-in-human-skin-fibroblasts
#9
Lukasz Szoka, Ewa Karna, Kornelia Hlebowicz-Sarat, Jacek Karaszewski, Jerzy A Palka
Abundance of proline (Pro) in collagen molecule led us to investigate whether Pro supply affects collagen biosynthesis in human skin fibroblasts. Treatment of the cells with milimolar concentrations (5 and 10 mM) of Pro for 24 and 48 h contributed to increase in α1 subunit of collagen type I (COL1A1) expression in both cells and culture medium. However, the effect was more pronounced in glutamine-free medium. In such condition, Pro induced collagen expression by about twofold in the cells, while in the medium only by about 30% during 24 h incubation, compared to control...
May 19, 2017: Molecular and Cellular Biochemistry
https://www.readbyqxmd.com/read/28526925/polyphenolic-extract-attenuates-fatty-acid-induced-steatosis-and-oxidative-stress-in-hepatic-and-endothelial-cells
#10
Laura Vergani, Giulia Vecchione, Francesca Baldini, Elena Grasselli, Adriana Voci, Piero Portincasa, Pier Francesco Ferrari, Bahar Aliakbarian, Alessandro A Casazza, Patrizia Perego
PURPOSE: Phenolic compounds (PC) of virgin olive oil exert several biochemical and pharmacological beneficial effects. Some dietary PC seem to prevent/improve obesity and metabolic-related disorders such as non-alcoholic fatty liver disease (NAFLD). We investigated the possible effects of PC extracted from olive pomace (PEOP) and of the main single molecules present in the extract (tyrosol, apigenin, oleuropein, p-coumaric and caffeic acid) in protecting hepatocytes and endothelial cells against triglyceride accumulation and oxidative stress...
May 19, 2017: European Journal of Nutrition
https://www.readbyqxmd.com/read/28526628/heparin-based-hydrogels-induce-human-renal-tubulogenesis-in-vitro
#11
Heather M Weber, Mikhail V Tsurkan, Valentina Magno, Uwe Freudenberg, Carsten Werner
Dialysis or kidney transplantation is the only therapeutic option for end stage renal disease. Accordingly, there is a large unmet clinical need for new causative therapeutic treatments. Obtaining robust models that mimic the complex nature of the human kidney is a critical step in the development of new therapeutic strategies. Here we establish a synthetic in vitro human renal tubulogenesis model based on a tunable glycosaminoglycan-hydrogel platform. In this system, renal tubulogenesis can be modulated by the adjustment of hydrogel mechanics and degradability, growth factor signaling, and the presence of insoluble adhesion cues, potentially providing new insights for regenerative therapy...
May 16, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/28526609/effects-of-l-dopa-benserazide-co-treatment-on-colonic-excitatory-cholinergic-motility-and-enteric-inflammation-following-dopaminergic-nigrostriatal-neurodegeneration
#12
C Pellegrini, L Antonioli, R Colucci, E Tirotta, D Gentile, C Ippolito, C Segnani, G Levandis, S Cerri, R F Blandini, E Barocelli, V Ballabeni, N Bernardini, C Blandizzi, M Fornai
INTRODUCTION: The mainstay therapy for Parkinson's disease (PD) relies on L-3,4- dihydroxyphenylalanine (L-DOPA) plus a DOPA-decarboxylase inhibitor. However, their effects on colonic dysmotility and inflammation observed in PD are undetermined. This study examined the effects of L-DOPA plus benserazide (BE) on colonic motility and inflammation in rats with central nigrostriatal dopaminergic denervation. METHODS: Neurodegeneration was induced by 6-hydroxydopamine (6-OHDA) injection into the median forebrain bundle (MFB)...
May 16, 2017: Neuropharmacology
https://www.readbyqxmd.com/read/28526426/the-effects-of-troglitazone-on-ampk-in-hepg2-cells
#13
Katherine M Allen, Kimberly A Coughlan, Fabliha N Mahmood, Rudy J Valentine, Neil B Ruderman, Asish K Saha
AMP-activated protein kinase (AMPK) is an enzyme crucial in cellular metabolism found to be inhibited in many metabolic diseases including type 2 diabetes. Thiazolidinediones (TZDs) are a class of anti-diabetic drug known to activate AMPK through increased phosphorylation at Thr(172), however there has been no research to date on whether they have any effect on inhibition of AMPK's lesser known site of inhibition, Ser(485/491). HepG2 cells were treated with troglitazone and phosphorylation of AMPK was found to increase at both Thr(172) and Ser(485) in a dose- and time-dependent manner...
May 16, 2017: Archives of Biochemistry and Biophysics
https://www.readbyqxmd.com/read/28526369/quorum-sensing-modulators-exhibit-cytotoxicity-in-hodgkin-s-lymphoma-cells-and-interfere-with-nf-%C3%AE%C2%BAb-signaling
#14
Natarajan Nandakumar, Rambabu Dandela, Jacob Gopas, Michael M Meijler
In recent years it has become evident that bacteria can modulate signaling pathways in host cells through the secretion of small signaling molecules. We have evaluated the cytotoxic effects and NF-κB inhibitory activities of a panel of quorum sensing molecules and their reactive analogs on Hodgkin's lymphoma cells (L428). We found that several molecules inhibited NF-κB signaling in a dose dependent manner. Three inhibitors (ITC-12, ITC-Cl and Br-Furanone) showed 50% NF-κB inhibition at concentrations less than 10µM (4...
May 6, 2017: Bioorganic & Medicinal Chemistry Letters
https://www.readbyqxmd.com/read/28526203/quantitative-evaluation-of-inhibitory-effect-of-various-substances-on-anaerobic-ammonia-oxidation-anammox
#15
Tomotaka Nakamura, Yuhki Harigaya, Yuya Kimura, Megumi Kuroiwa, Yuhri Kurata, Kazuichi Isaka, Yuichi Suwa
The inhibitory effect of 20 substances of various chemical species on the anaerobic ammonia oxidation (anammox) activity of an enrichment culture, predominated by Candidatus Brocadia, was determined systematically by using a (15)N tracer technique. The initial anammox rate was determined during first 25 min with a small-scale anaerobic batch incubation supplemented with possible inhibitors. Although Cu(2+) and Mn(2+) did not inhibit anammox, the remaining 18 substances [Ni(2+), Zn(2+), Co(2+), [Formula: see text] , Fe(2+), 4 amines, ethylenediaminetetraacetic acid (EDTA), ethylenediamine-N,N'-bis (2-hydroxyphenylacetic acid) (EDDHA), citric acid, nitrilotriacetic acid (NTA), N,N-dimethylacetamide (DMA), 1,4-dioxane, dimethyl sulfoxide (DMSO), N,N-dimethylformamide (DMF) and tetrahydrofuran (THF)] were inhibitory...
May 16, 2017: Journal of Bioscience and Bioengineering
https://www.readbyqxmd.com/read/28525956/aggregation-induced-emission-fluorogens-light-cells-via-microtubules-accessing-the-mechanisms-of-intracellular-trafficking-of-ionic-substances
#16
Shixin Zhou, Yijun Xia, Yinan Liu, Qihua He, Bo Song
Understanding the enrichment and intracellular trafficking of substances is centrally important to the biological systems. Here, employing an amphiphilic molecule (denoted by TPE-11) bearing tetraphenylethene moiety, known for aggregation induced emission property, we demonstrated its localization shifting in Hela cells after prolonged incubation. Through a set of delicately designed experiments, we found that one type of cytoskeleton, i.e. microtubule, is responsible for the intracellular transportation regardless of the sources of fluorogens, via endocytosis pathways or not...
May 19, 2017: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/28525898/ginsenoside-rg3-inhibits-the-senescence-of-prostate-stromal-cells-through-down-regulation-of-interleukin-8-expression
#17
Yanfei Peng, Ran Zhang, Lingfei Kong, Yongmei Shen, Da Xu, Fang Zheng, Jianwei Liu, Qian Wu, Bona Jia, Ju Zhang
Senescent stromal cells support the development of prostate cancer and are considered potential therapeutic targets. This research evaluated the regulatory effects of ginsenoside Rg3 on the senescence of prostatic stromal cells pre-incubated in medium supplemented with 0.5% fetal bovine serum. The results revealed that ginsenoside Rg3 decreased the number of stromal cells positively stained with a senescent cell marker (senescence-associated β-galactosidase). Ginsenoside Rg3 also increased the viability of stromal cells and promoted cell cycle transition from G0/G1 to S phase, as well as inhibited the carcinoma-associated fibroblast-like phenotype in prostate stromal cells, through the up-regulation of smooth muscle cell markers SM22 and smooth muscle myosin heavy chain...
May 4, 2017: Oncotarget
https://www.readbyqxmd.com/read/28525869/hepatic-microsomal-metabolism-of-bde-47-and-bde-99-by-lesser-snow-geese-and-japanese-quail
#18
Lisa K Krieger, András Szeitz, Stelvio M Bandiera
In the present study, we investigated the oxidative biotransformation of 2,2',4,4'-tetrabromodiphenyl ether (BDE-47) and 2,2',4,4',5-pentabromodiphenyl ether (BDE-99) by liver microsomes from wild lesser snow geese (Chen caerulescens caerulescens) and domesticated Japanese quail (Coturnix japonica). Formation of hydroxy-metabolites was analyzed using an ultra-high performance liquid chromatography-tandem mass spectrometry-based method. Incubation of BDE-47 with avian liver microsomes produced sixteen hydroxy-metabolites, eight of which were identified using authentic standards...
May 10, 2017: Chemosphere
https://www.readbyqxmd.com/read/28525726/characterization-of-an-ionic-liquid-tolerant-%C3%AE-xylosidase-from-a-marine-derived-fungal-endophyte
#19
Anindita Sengupta, Angela Zabala, Si Yu Tan, Arthur Broadstock, T S Suryanarayanan, Venkat Gopalan
Ionic liquids (ILs) are used in lignocellulosic biomass (LCB) pretreatment due to their ability to disrupt the extensive hydrogen-bonding network in cellulose and hemicellulose, and thereby decrease LCB recalcitrance to subsequent enzymatic degradation. However, this approach necessitates development of a cellulases and hemicellulases that can tolerate ~20% (w/v) IL, an amount that either co-precipitates with the sugar polymers after the initial pretreatment or is typically used in single-pot biomass deconstructions...
May 19, 2017: Biochemistry and Cell Biology, Biochimie et Biologie Cellulaire
https://www.readbyqxmd.com/read/28525327/in-vitro-fertilizing-potential-of-urethral-and-epididymal-spermatozoa-collected-from-domestic-cats-felis-catus
#20
S Prochowska, W Niżański
The aim of this study was to provide a comparative analysis of in vitro fertilizing potential of frozen-thawed urethral and epididymal feline spermatozoa. Both types of semen were collected from 7 cats and cryopreserved in liquid nitrogen. To perform in vitro fertilization, both urethral and epididymal samples from the same individual were thawed and spermatozoa were co-incubated with in vitro matured cat oocytes. Obtained embryos were cultured in vitro for 7 days in a commercial medium. Cleavage rate, morula rate and blastocyst rate were calculated...
March 28, 2017: Polish Journal of Veterinary Sciences
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