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Spectrophotometric estimation

Hongfu Yang, Ke Chao, Ming Liang, Rongqing Sun
OBJECTIVE: To investigate the effects of pyrrolidine dithiocarbamate (PDTC) on oxidative stress and mitochondrial function of lung tissue in mice with acute lung injury (ALI) induced by lipopolysaccharide (LPS). METHODS: Forty female Balb/c mice were randomly divided into normal saline (NS) control group, LPS model group, PDTC group, and PDTC+LPS group, with 10 mice in each group. The model of mice with ALI was reproduced by intraperitoneal injection of 15 mg/kg LPS...
May 2018: Zhonghua Wei Zhong Bing Ji Jiu Yi Xue
Germana Bancone, Gornpan Gornsawun, Cindy S Chu, Pen Porn, Sampa Pal, Pooja Bansil, Gonzalo J Domingo, Francois Nosten
INTRODUCTION: Glucose-6-phosphate dehydrogenase (G6PD) deficiency is the most common enzymopathy in the human population affecting an estimated 8% of the world population, especially those living in areas of past and present malaria endemicity. Decreased G6PD enzymatic activity is associated with drug-induced hemolysis and increased risk of severe neonatal hyperbilirubinemia leading to brain damage. The G6PD gene is on the X chromosome therefore mutations cause enzymatic deficiency in hemizygote males and homozygote females while the majority of heterozygous females have an intermediate activity (between 30-80% of normal) with a large distribution into the range of deficiency and normality...
2018: PloS One
Masumeh Zamanlu, Morteza Eskandani, Reza Mohammadian, Nazila Entekhabi, Mohammad Rafi, Mehdi Farhoudi
Introduction: Measurement of thrombolytic activity is crucial for research and development of novel thrombolytics. It is a key factor in the assessment of the effectiveness of conventionally used thrombolytic therapies in the clinic. Previous methods used for the assessment of thrombolytic activity are often associated with some drawbacks such as being costly, time-consuming, complex with low accuracy. Here, we introduce a simple, economic, relatively accurate and fast method of spectrophotometric analysis of thrombolytic activity (SATA) assay, standardized by tissue plasminogen activator (tPA), which can quantitatively measure in vitro thrombolytic activity...
2018: BioImpacts: BI
Mohamed A Korany, Hoda Mahgoub, Rim S Haggag, Marwa A A Ragab, Osama A Elmallah
A green, simple and cost effective chemometric UV-Vis spectrophotometric method has been developed and validated for correcting interferences that arise during conducting biowaiver studies. Chemometric manipulation has been done for enhancing the results of direct absorbance, resulting from very low concentrations (high incidence of background noise interference) of earlier points in the dissolution timing in case of dissolution profile using first and second derivative (D1 & D2) methods and their corresponding Fourier function convoluted methods (D1/FF& D2/FF)...
March 27, 2018: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
S R Prabhukarthikeyan, U Keerthana, T Raguchander
Rhizome rot of turmeric caused by Pythium aphanidermatum is a major threat to turmeric-cultivating areas of India. This study intends to evaluate the performance of fluorescent pseudomonads against Rhizome rot disease and understand the resistance mechanism in Turmeric plants. Fluorescent pseudomonads were screened against Pythium aphanidermatum using dual culture. Selected strains were evaluated for the performance of growth promoting attributes and the presence of antibiotic genes through PCR analysis. Strain FP7 recorded the maximum percent inhibition of P...
May 2018: Microbiological Research
Sudhindra Baliga, Minal Chaudhary, Sham Bhat, Pooja Bhansali, Akshat Agrawal, Satwik Gundawar
Background: Sickle cell anemia (SCA) is an inherited disorder of hemoglobin synthesis characterized by deformed erythrocytes. Hemoglobin S present in sickle-shaped erythrocytes exhibits an enhanced rate of auto-oxidation compared with normal hemoglobin A. It produces more of reactive oxygen species (ROS) which promotes oxidatively stressed environment. ROS degrade the membranes of sickle cell erythrocytes composed of polyunsaturated lipids and form malondialdehyde (MDA) as a by-product...
January 2018: Journal of the Indian Society of Pedodontics and Preventive Dentistry
Ewa Grela, Joanna Kozłowska, Agnieszka Grabowiecka
The MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) tetrazolium assay is a popular tool in estimating the metabolic activity of living cells. The test is based on enzymatic reduction of the lightly colored tetrazolium salt to its formazan of intense purple-blue color, which can be quantified spectrophotometrically. Under properly optimized conditions the obtained absorbance value is directly proportional to the number of living cells. Originally, the MTT assay was devised for use in eukaryotic cells lines and later applied for bacteria and fungi...
March 29, 2018: Acta Histochemica
Michael L Sullivan, Nicholas D Bonawitz
Hydroxycinnamoyl-Coenzyme A (CoA) hydroxycinnamoyl transferases are BAHD family acyltransferases that transfer hydroxycinnamoyl moieties from a CoA-thioester to an acceptor amine or alcohol to form an N-hydroxycinnamoyl amide or O-hydroxycinnamoyl ester, respectively, with the concomitant release of free CoA. One approach to measure reaction rates for these enzymes is to quantify the hydroxycinnamoyl amide or ester reaction product following chromatographic separation of reaction components. This approach can be labor-intensive and time-consuming...
April 2018: Plant Science: An International Journal of Experimental Plant Biology
F Belal, F Ibrahim, Z A Sheribah, H Alaa
In this paper, novel univariate and multivariate regression model updating methods were developed and validated for the simultaneous determination of ternary mixture of bromazepam, diazepam and clonazepam. The univariate methods used are the mean centering of ratio spectra spectrophotometric method and iso-absorptive point coupled with ratio-subtraction and ratio difference spectrophotometric method. Linearity, ranges, precision and accuracy of these methods were determined. The multivariate methods adopted are the principal component regression and partial least-squares...
March 27, 2018: Clinica Chimica Acta; International Journal of Clinical Chemistry
Govind Singh, Shrimanjunath Sankanagoudar, Premnath Dogra, Nimai Chand Chandra
Background & objectives: Earlier reports have shown hypocholesterolaemia in cancer patients and high number of lipid rafts in cancer cells. The primary objective of this study was to compare the intracellular cholesterol turnover in non-cancerous (benign) prostatic hyperplasia (BPH) and carcinoma prostate (CAP) with normal prostate cells obtained from patients undergoing radical cystectomy for carcinoma bladder (sham control). Methods: ELISA-based estimation of prostate-specific antigen (PSA), evaluation of expression of low-density lipoprotein receptor (LDLR), peripheral-type benzodiazepine receptor (PBR) and cyclin E, immunohistochemistry and confocal microscopy, measurement of integrated optical density of the diaminobenzidine (DAB)-stained immunohistograms, isolation of nucleus and cell cytoplasm from prostate tissue by ultracentrifugation followed by estimation of cholesterol spectrophotometrically in isolated nuclear and cytoplasmic fractions were performed...
November 2017: Indian Journal of Medical Research
Refilwe Given Kudumela, Peter Masoko
There is very little scientific information on indigenous plants used for medicinal purposes. Therefore, the aim of the study was to evaluate the antibacterial and antioxidant activities of the leaves of Commelina africana, Elephantorrhiza elephantina, Dombeya rotundifolia, and the whole plant excluding the roots of Schkuhria pinnata indigenous medicinal plants from the Limpopo Province, which may be used for the treatment in humans infected with bacterial pathogens. The screening of different phytoconstituents using standard methods revealed the presence of terpenoids, flavonoids, tannins, and saponins...
January 2018: Journal of evidence-based integrative medicine
Hamid Reza Rajabi, Fatemeh Karimi, Hossein Kazemdehdashti, Leila Kavoshi
In this research, a sono-assisted chemical precipitation method was developed for a quick and efficient water-based synthesis of zinc sulfide (ZnS) quantum dots (QDs), in room temperature. ZnS QDs as pure and doped with some transition metal ions (such as Cu2+ , Mn2+ , Ag+ ) were prepared in the presence of l-cysteine, as capping agent. In comparison with the previous conventional methods, applying the ultrasonic irradiation provides a significant decrease in the reaction time. In comparison with previous reported conventional chemical QD synthesis methods, the required time for a complete synthesis in the presence of ultrasonic irradiations was significantly reduced to a period as short as 15 min, in a temperature of 40 °C...
February 22, 2018: Journal of Photochemistry and Photobiology. B, Biology
Łukasz Murlikiewicz, Krzysztof Grzegorczyk, Małgorzata Lewicka, Andrzej Buczyński, Maciej Rutkowski
BACKGROUND: Thus far, the pathogenesis of these intestinal tumors has not been fully explained. However, the analysis of risk factors and research regarding their formation that have continued for 3 decades have allowed us to demonstrate a significant role of oxidative stress in the processes leading to the development of cancer in the large intestine as well as in some other organs. OBJECTIVES: The aim of the study was to examine the level of anti-oxidative status and the degree of oxidative protein damage in patients with varying severity of colonic adenocarcinoma (CAC) in relation to healthy individuals...
January 2018: Advances in Clinical and Experimental Medicine: Official Organ Wroclaw Medical University
Vineet K Singh, Sirisha Sirobhushanam, Robert P Ring, Saumya Singh, Craig Gatto, Brian J Wilkinson
PURPOSE: Membrane fluidity to a large extent is governed by the presence of branched-chain fatty acids (BCFAs). Branched-chain α-keto acid dehydrogenase (BKD) is the key enzyme in BCFA synthesis. A Staphylococcus aureus BKD-deficient strain still produced substantial levels of BCFAs. Pyruvate dehydrogenase (PDH) with structural similarity to BKD has been speculated to contribute to BCFAs in S. aureus. METHODOLOGY: This study was carried out using BKD-, PDH- and BKD : PDH-deficient derivatives of methicillin-resistant S...
April 2018: Journal of Medical Microbiology
Kazuhide Miyamoto, Ayumi Yamashita, Kazuki Saito
Cross-brace structural motifs are required as a scaffold to design artificial RING fingers (ARFs) that function as ubiquitin ligase (E3) in ubiquitination and have specific ubiquitin-conjugating enzyme (E2)-binding capabilities. The Simple Modular Architecture Research Tool database predicted the amino acid sequence 131-190 (KIAA1045ZF) of the human KIAA1045 protein as an unidentified structural region. Herein, the stoichiometry of zinc ions estimated spectrophotometrically by the metallochromic indicator revealed that the KIAA1045ZF motif binds to two zinc atoms...
May 2018: Protein Science: a Publication of the Protein Society
Maria Ashfaq, Ali Akber Sial, Rabia Bushra, Atta-Ur Rehman, Mirza Tasawur Baig, Ambreen Huma, Maryam Ahmed
Spectrophotometric technique is considered to be the simplest and operator friendly among other available analytical methods for pharmaceutical analysis. The objective of the study was to develop a precise, accurate and rapid UV-spectrophotometric method for the estimation of chlorpheniramine maleate (CPM) in pure and solid pharmaceutical formulation. Drug absorption was measured in various solvent systems including 0.1N HCl (pH 1.2), acetate buffer (pH 4.5), phosphate buffer (pH 6.8) and distil water (pH 7...
January 2018: Pakistan Journal of Pharmaceutical Sciences
Anita S Iyer, Andrew S Azman, Malika Bouhenia, Lul O Deng, Cole P Anderson, Michael Graves, Pavol Kováč, Peng Xu, Edward T Ryan, Jason B Harris, David A Sack, Francisco J Luquero, Daniel T Leung
BACKGROUND: Vibrio cholerae causes over 2 million cases of cholera and 90,000 deaths each year. Serosurveillance can be a useful tool for estimating the intensity of cholera transmission and prioritizing populations for cholera control interventions. Current methods involving venous blood draws and downstream specimen storage and transport methods pose logistical challenges in most settings where cholera strikes. To overcome these challenges, we developed methods for determining cholera-specific immune responses from dried blood spots (DBS)...
January 2018: PLoS Neglected Tropical Diseases
Alma Betsaida Benitez-Trinidad, Irma Martha Medina-Díaz, Yael Yvette Bernal-Hernández, Briscia Socorro Barrón-Vivanco, Cyndia Azucena González-Arias, José Francisco Herrera-Moreno, Isabel Alvarado-Cruz, Betzabet Quintanilla-Vega, Aurora Elizabeth Rojas-García
Recently a relationship has been reported between pesticide exposure and changes in global DNA methylation patterns. Urban sprayers are a particularly vulnerable population because of the high risk of pesticide exposure that their work implies. Therefore, the aim of this study was to estimate the changes in the Long Interspersed Nucleotide Element (LINE-1) in urban sprayers and its relationship with pesticide exposure. The study population consisted of 190 individuals stratified into three study groups: no occupational pesticide exposure; moderate exposure, and high exposure...
March 2018: Food and Chemical Toxicology
Eliezer Oliveira, Camelia Henriquez, Lidiane C Nunes, Elias A G Zagatto
Multivariate calibration involving partial least squares was exploited in the flow-based spectrophotometric determination of molybdenum in river waters relying on the Mo(VI)-catalyzed iodide oxidation by H2 O2 under acidic conditions. Two sample aliquots were simultaneously inserted into the carrier stream, and differential pumping was accountable for in-line addition of sulfuric acid to one of them. Pronounced gradients (acidity and reagent concentrations) were established along the complex sample zone formed, and the absorbance-time function was characterized by local maximum and minimum values...
March 1, 2018: Talanta
Karel Van Keer, Jan Van Keer, João Barbosa Breda, Vahid Nassiri, Cathy De Deyne, Cornelia Genbrugge, Luís Abegão Pinto, Ingeborg Stalmans, Evelien Vandewalle
BACKGROUND: To investigate the correlation between cerebral (SO2-transcranial), retinal arterial (SaO2-retinal) and venous (SvO2-retinal) oxygen saturation as measured by near-infrared spectroscopy (NIRS) and retinal oximetry respectively. METHODS: Paired retinal and cerebral oxygen saturation measurements were performed in healthy volunteers. Arterial and venous retinal oxygen saturation and diameter were measured using a non-invasive spectrophotometric retinal oximeter...
2018: PloS One
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