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Spectrophotometric estimation

Emmanuel Amabebe, Steven Reynolds, Victoria Stern, Graham Stafford, Martyn Paley, Dilly O C Anumba
Changes in vaginal microbiota that is associated with preterm birth (PTB) leave specific metabolite fingerprints that can be detected in the cervicovaginal fluid (CVF) using metabolomics techniques. In this study, we characterize and validate the CVF metabolite profile of pregnant women presenting with symptoms of threatened preterm labor (PTL) by both (1)H-nuclear magnetic resonance spectroscopy (NMR) and enzyme-based spectrophotometry. We also determine their predictive capacity for PTB, singly, and in combination, with current clinical screening tools - cervicovaginal fetal fibronectin (FFN) and ultrasound cervical length (CL)...
2016: Frontiers in Medicine
Ritu Tiwari, Chaya Manoranjini David, Datturao Ramanand Mahesh, Uma Sambargi, Kademadkal Javaraiah Rashmi, Prabhavati Benakanal
Potentially malignant disorders (PMDs) of oral cavity and oral cancer remain a cause of serious concern despite intensive research and development. Diet and immunity have been identified to play a crucial role as modifying factors in these diseases. Our study intended to explore this relationship by estimating and comparing the serum levels of copper, iron and circulating immune complexes (CICs) in patients diagnosed with PMDs and oral cancer and normal healthy individuals. In this study, 40 histopathologically diagnosed cases of PMDs and oral cancer were included along with 30 healthy controls and 5 ml of venous blood was drawn using venipuncture...
October 10, 2016: Brazilian Oral Research
Firoj A Tamboli, Harinath N More
BACKGROUND: Peptic ulcer is a digestive disorder most commonly found in clinical practice. Given the many side effects of modern medicine, the initial acquisition of fewer side effects, and medication of indigenous drugs, it should be considered as a better alternative for the treatment of peptic ulcer. OBJECTIVE: To assess antiulcer and antioxidant activity of ethanol extract of Barleria gibsoni (EBG) Dalz. leaves in ulcer-induced rats and in vitro antioxidants method, respectively...
October 2016: Pharmacognosy Research
Arun Sankaralingam, Annette Thomas, David James, Anthony S Wierzbicki
BACKGROUND: Ethylene glycol (EG) poisoning remains a rare but important presentation to acute toxicology units. Guidelines recommended that Ethylene Glycol should be available as an 'urgent' test within 4 hours but these are difficult to deliver in practice. This study assessed a semi-quantitative enzymatic spectrophotometric assay for EG compatible with automated platforms. METHODS: The EG method was assessed in 21 samples from patients with an increased anion gap and metabolic acidosis not due to EG ingestion, and 7 samples known to contain EG...
September 28, 2016: Annals of Clinical Biochemistry
Adriana Passarella Gerola, Flavia Amanda Pedroso de Morais, Paulo Fernando A Costa, Elza Kimura, Wilker Caetano, Noboru Hioka
The spectrophotometric properties of chlorophylls' derivatives (Chls) formulated in the Pluronics® F-127 and P-123 were evaluated and the results have shown that the Chls were efficiently solubilized in these drug delivery systems as monomers. The relative location of the Chls in the Pluronics® was estimated from the Stokes shift and micropolarity of the micellar environment. Chls with phytyl chain were located in the micellar core, where the micropolarity is similar to ethanol, while phorbides' derivatives (without phytyl chain) were located in the outer shell of the micelle, i...
September 19, 2016: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
P Sahare, M Ayala, R Vazquez-Duhalt, U Pal, A Loni, L T Canham, I Osorio, V Agarwal
The study of the stability enhancement of a peroxidase immobilized onto mesoporous silicon/silica microparticles is presented. Peroxidases tend to get inactivated in the presence of hydrogen peroxide, their essential co-substrate, following an auto-inactivation mechanism. In order to minimize this inactivation, a second protein was co-immobilized to act as an electron acceptor and thus increase the stability against self-oxidation of peroxidase. Two heme proteins were immobilized into the microparticles: a fungal commercial peroxidase and cytochrome c from equine heart...
December 2016: Nanoscale Research Letters
T M Ripp, T Yu Rebrova, V F Mordovin, S A Afanas'ev, S E Pekarsky, G V Semke, A Yu Fal'kovskaya, V A Lichikaki, E V Tsymbalyuk
AIM: to define and develop a procedure that can select patients with resistant hypertension (RH) for a renal sympathetic denervation (RSD) procedure, by being orienting to the specific initial values of β-adrenoreactivity (β-AR) and systolic blood pressure (SBP). SUBJECTS AND METHODS: The analysis included 23 RH patients receiving the maximally tolerable doses of 4 antihypertensive drugs. The investigations involving BP control and a Russian spectrophotometric procedure for determining β-AR in terms of the change in the osmoresistance of erythrocyte membranes (EM) were performed at baseline and 4 and 24 weeks...
2016: Terapevticheskiĭ Arkhiv
Mohammad Normohammadi, Hossein Kakooei, Leila Omidi, Saeed Yari, Rasul Alimi
BACKGROUND: Building demolition can lead to emission of dust into the environment. Exposure to silica dust may be considered as an important hazard in these sites. The objectives of this research were to determine the amount of workers' exposure to crystalline silica dust and assess the relative risk of silicosis and the excess lifetime risk of mortality from lung cancer in demolition workers. METHODS: Four sites in the Tehran megacity region were selected. Silica dust was collected using the National Institute for Occupational Safety and Health method 7601 and determined spectrophotometrically...
September 2016: Safety and Health At Work
Lohith Karnam, Sunil Adurty, Siva Kumar Belliraj, Sundaresan Neelakantan Chittor
A one-pot, cost-effective system for sensing fluoride ions using quinizarin as a novel chromophore is reported. Quinizarin complexes with Al(III) to form a self-organized turn-on fluorescence system in methanol. In the presence of fluoride ions, this system undergoes a subsequent ligand swap reaction with the fluoride ion and consequently the fluorescence turns off, delivering a sensitive route for the estimation of fluoride. This domino effect presents a sensitive and selective reaction system for the detection of fluoride ions in the concentration range 0...
September 14, 2016: Journal of AOAC International
Małgorzata M Godala, Izabela Materek-Kuśmierkiewicz, Dariusz Moczulski, Maciej Rutkowski, Franciszek Szatko, Ewelina Gaszyńska, Sławomir Tokarski, Jan Kowalski
BACKGROUND: Metabolic syndrome (MS) is a coexistence of metabolic risk factors affecting the development of cardiovascular diseases. Reactive oxygen species, which are excessively produced in MS, participate in its pathogenesis. Vitamins A, C and E are an important part of the non-enzymatic antioxidative barrier in humans. OBJECTIVES: The aim of the study was to estimate plasma vitamin A, C and E levels and the intake of these vitamins from the diet in patients with MS...
July 2016: Advances in Clinical and Experimental Medicine: Official Organ Wroclaw Medical University
Hari Shanker Tiwari, Usha Kant Misra, Jayantee Kalita, Akanksha Mishra, Shubha Shukla
Cerebral venous sinus thrombosis (CVST) is an important cause of stroke in young especially in the developing countries. There is paucity of studies on the mechanism of cell damage in CVST. Aim of this study is to explore the role of glutamate excitotoxicity, oxidative stress; and apoptosis in an experimental model of CVST. Cerebral venous sinus thrombosis was induced by putting a strip of filter paper soaked in 40% ferric chloride on superior sagittal sinus. Brain was removed on day 1, 2 and 7 and oxidative stress markers (Mn-SOD, Catalase, GPx, LPO and GSH) were estimated spectrophotometrically...
September 19, 2016: Neurochemistry International
C T Sulaiman, Indira Balachandran
Characterisation of antioxidant fraction of the fruit of Garcinia gummi-gutta was done using liquid chromatography mass spectroscopic (LC/MS) analyses. Total poly phenolics and radical scavenging activity of various extracts such as acetone, methanol and hydroalcohol were estimated spectrophotometrically. The active extract was analysed by LC/MS in order to identify the molecular mass and tentative structures of major compounds. Phenolic compounds such as luteolin 7-O-glucuronide, kaempferol 3-O-(6-O-acetyl) glycoside, dicaffeoylquinic acid, apigenein-6-C-pentosyl-8-C-hexoside and p-coumarylquinic acid were identified from hydro alcoholic extract of G...
September 1, 2016: Natural Product Research
Sujuan Guo, Kevin J Pridham, Zhi Sheng
Autophagy is a catabolic process whereby cellular components are degraded to fuel cells for longer survival during stress. Hence, autophagy plays a vital role in determining cell fate and is central for homeostasis and pathogenesis of many human diseases including chronic myeloid leukemia (CML). It has been well established that autophagy is important for the leukemogenesis as well as drug resistance in CML. Thus, autophagy is an intriguing therapeutic target. However, current approaches that detect autophagy lack reliability and often fail to provide quantitative measurements...
2016: Methods in Molecular Biology
Veena D Singh, Sanjay J Daharwal
Three multivariate calibration spectrophotometric methods were developed for simultaneous estimation of Paracetamol (PARA), Enalapril maleate (ENM) and Hydrochlorothiazide (HCTZ) in tablet dosage form; namely multi-linear regression calibration (MLRC), trilinear regression calibration method (TLRC) and classical least square (CLS) method. The selectivity of the proposed methods were studied by analyzing the laboratory prepared ternary mixture and successfully applied in their combined dosage form. The proposed methods were validated as per ICH guidelines and good accuracy; precision and specificity were confirmed within the concentration range of 5-35μgmL(-1), 5-40μgmL(-1) and 5-40μgmL(-1)of PARA, HCTZ and ENM, respectively...
January 15, 2017: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
M M Elimam, S W Shantier, E A Gadkariem, M A Mohamed, Z Osman
OBJECTIVES: This study aims to investigate the stability of florfenicol using previously developed derivative spectrophotometric methods (D(1) and D(2)). METHODS: The studied stability-indicating pararmeters included alkali (NaOH, 1M), acid (HCl, 1M), pH changes (buffer pH 2.2-11), temperature (80°C and 100°C at pH 10) and light. RESULT: A constructed pH profile for the drug degradation rate revealed a significant effect of pH on the drug stability between pH ranges 8 and 11...
August 24, 2016: Annales Pharmaceutiques Françaises
Mainak Chakraborty, Indrajit Karmakar, Sagnik Haldar, Avratanu Das, Asis Bala, Pallab Kanti Haldar
INTRODUCTION: The present study evaluates the antioxidant effect of methanol extract of Hippophae salicifolia (MEHS) bark with special emphasis on its role on oxidative DNA damage in mouse peritoneal macrophages. MATERIAL AND METHODS: In vitro antioxidant activity was estimated by standard antioxidant assays whereas the antioxidant activity concluded the H(+) donating capacity. Mouse erythrocytes' hemolysis and peritoneal macrophages' DNA damage were determined spectrophotometrically...
July 2016: Journal of Pharmacy & Bioallied Sciences
Shankara H Nanjegowda, Manasa G Papanna, Raghu Ram Achar, Kanchugarakoppal S Rangappa, Puttaswamappa Mallu, Shivananju Nanjunda Swamy
A new simple, rapid and precise RP-HPLC method was developed for the extraction and quantitative estimation of caffeine (C), (-)-epigallocatechin gallate (EGCG), (+)-catechin(Ct), (-)-epicatechin(EC), and (-)-epicatechin gallate (ECG) (collectively named as Tea Powder Bioactives TPBAs) extracted from tea powder using different ratios of ethanol: water. The simultaneous determination of TPBAs was performed using the UV spectrophotometric method which employs the absorbance at 205 nm (λmax of caffeine and polyphenols)...
May 2016: Journal of Food Science and Technology
Fawzi A El-Yazbi, Omayma A Amin, Eman I El-Kimary, Essam F Khamis, Sameh E Younis
An accurate, precise, rapid, specific and economic high-performance thin-layer chromatographic (HPTLC) method has been developed for the simultaneous quantitative determination of febuxostat (FEB) and diclofenac potassium (DIC). The chromatographic separation was performed on precoated silica gel 60 GF254 plates with chloroform-methanol 7:3 (v/v) as the mobile phase. The developed plates were scanned and quantified at 289 nm. Experimental conditions including band size, mobile phase composition and chamber-saturation time were critically studied, and the optimum conditions were selected...
August 2016: Journal of Chromatographic Science
M Abdulla Al-Mamun, Zerin Akter, Md Josim Uddin, K M K B Ferdaus, K M F Hoque, Z Ferdousi, M Abu Reza
BACKGROUND: Ricinus communis (Euphorbiaceae) has previously been reported to possess analgesic, antihistamine, antioxidant and anti-inflammatory activities. This study was designed for isolation, characterization and evaluation of antibacterial and anti-proliferative activities of R. communis seed protein. METHODS: The concentration and molecular weight of R. communis seed protein were estimated by SDS-PAGE and spectrophotometric analysis, respectively. Lectin activity was evaluated by hemagglutination assay on mice blood...
2016: BMC Complementary and Alternative Medicine
Maurício Liberal de Almeida, Cassiano Junior Saatkamp, Adriana Barrinha Fernandes, Antonio Luiz Barbosa Pinheiro, Landulfo Silveira
Urea and creatinine are commonly used as biomarkers of renal function. Abnormal concentrations of these biomarkers are indicative of pathological processes such as renal failure. This study aimed to develop a model based on Raman spectroscopy to estimate the concentration values of urea and creatinine in human serum. Blood sera from 55 clinically normal subjects and 47 patients with chronic kidney disease undergoing dialysis were collected, and concentrations of urea and creatinine were determined by spectrophotometric methods...
September 2016: Lasers in Medical Science
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